A microbial fluorescence detection system and detection method

Abstract

The present invention provides a microorganism fluorescence detection system and detection method, which relates to the field of microorganism gene detection technology. The detection system includes a body, a detection hole, a drive plate, a mounting plate, a holding bottle, a catheter and an automatic dripping mechanism. The detection hole is arranged on one side of the body, the drive plate is installed inside the detection hole, the mounting plate is installed on the body, and a plurality of holding bottles are installed on one side of the mounting plate, and one end of the catheter is connected to the holding bottle. The present invention can automatically control the liquid transfer gun to drip the liquid to be detected into multiple detection cavities of the detection dish. The automatic dripping process makes the experimental process more coherent and systematic, reduces the operation steps and time waste, improves the detection efficiency, reduces the error caused by human operation, and improves the reliability of the experimental results; and during the dripping process, the conveyor belt can seal and shield the detection cavity, thereby avoiding the detection liquid from sputtering during the dripping process, thereby facilitating the influence of sample loss and avoiding experimental environmental pollution.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial gene detection, and particularly relates to a microbial fluorescence detection system and a detection method. Background Art

[0002] Food safety is regarded as a global public health issue, and foodborne pathogenic bacteria are an important aspect of food safety. Vegetables and fruits are nutritious foods. Due to the high water content of vegetables, they are extremely susceptible to the infection of pathogenic microorganisms during processing and transportation, which can cause pathogenic diseases and endanger human health. Escherichia coli, Salmonella, Listeria monocytogenes, Staphylococcus aureus, four kinds of foodborne pathogenic bacteria, as well as norovirus and rotavirus are common pathogenic microorganisms carried by vegetables.

[0003] Vegetables are essential foods in daily life. The contamination of pathogenic microorganisms on vegetables is closely related to the health of consumers. Therefore, the detection of pathogenic microorganisms in vegetables, especially raw vegetables, is crucial for ensuring the health of the people.

[0004] Fluorescence imaging is an indispensable tool in biological research, especially in cell research. By staining cells or organelles with various fluorophores and magnifying and imaging them under a microscope, a large number of color-coded processes can be quantitatively characterized, and the research on cell gene expression can be achieved based on chromosome dynamics. Fluorophores have greatly improved cell research by introducing high quantum yield fluorescent dyes and multiplex staining methods into this field. This enables researchers to simultaneously study several organelles and their interactions in the same FOV (field of view) with high contrast. A microbial fluorescence detection system is a technology for detecting and quantifying microorganisms, mainly used for monitoring and detecting microbial contamination in foods such as bacteria and fungi.

[0005] The invention with the publication number of CN110823854B discloses a fluorescence spectrum detection system for microorganisms. The key points of its technical solution are: including a laser light source emitting laser onto a microbial sample to be measured carried on a light-transmitting platform, exciting a fluorescent dye to emit fluorescence; the light-transmitting platform is arranged on the focal plane of a microscopic lens group; the microscopic lens group magnifies the fluorescent light transmitted by the light-transmitting platform to form parallel light; a filter element filters the parallel light; a first condenser lens group condenses the parallel light to form a fluorescence image, and the fluorescence image is divided into several image units by an integral field unit, and the several image units are dispersed by a spectrometer; a detector detects the obtained spectral distribution information. This application does not need to scan the fluorescent light at each position within the field of view of the microscopic lens group one by one, realizes the synchronous dispersion of the fluorescent light within the field of view of the microscopic lens group, improves the observation efficiency of the fluorescence detection of microbial samples, is conducive to capturing the instantaneous changes of cells or organelles in microorganisms, and improves the accuracy of the research on microorganisms.

[0006] Regarding the relevant content above, the following technical defects are found:

[0007] When the microbial fluorescence detection system in the prior art is in use, the operator needs to first manually use a pipette to drop the liquid to be detected into multiple detection cavities on a detection dish, then place the detection dish containing the liquid to be detected into the instrument, and finally start the instrument to perform the microbial fluorescence detection operation. However, the manual dropping method will cause the following problems:

[0008] 1. The operator needs to manually control the pipette to drop the liquid to be detected into the detection dish. Manually controlling the pipette to drop the liquid to be detected consumes the operator's time and energy, increases the complexity of the operation, and will affect the efficiency and accuracy of the detection;

[0009] 2. During the process of dropping the detection liquid into the detection dish, the detection liquid is prone to splashing, which will not only cause sample loss and affect the reliability of the experimental results, but also easily lead to environmental pollution in the laboratory, increasing the workload of cleaning and maintenance. Summary of the Invention

[0010] The purpose of the present invention is to solve the shortcomings existing in the prior art, and a microbial fluorescence detection system is proposed.

[0011] To achieve the above object, the present invention adopts the following technical solution: A microbial fluorescence detection system includes a body, a detection hole, a driving plate, a mounting plate, a storage bottle, a conduit, and an automatic liquid dropping mechanism. The detection hole is provided on one side of the body. The driving plate is installed inside the detection hole. The mounting plate is installed on the body. A plurality of storage bottles are installed on one side of the mounting plate. One end of the conduit is communicated with the storage bottle. The automatic liquid dropping mechanism is located on one side of the body. The automatic liquid dropping mechanism includes a support plate, a detection dish, and an automatic pipette. A plurality of detection cavities are formed on the upper surface of the detection dish. The detection cavities are evenly distributed on the upper surface of the detection dish. The lower surface of the detection dish and the upper surface of the driving plate are in the same plane. One side of the support plate is fixedly connected to the body. A servo motor is fixedly connected to one side of the support plate. A sliding hole is formed on the upper surface of the support plate. A slider is slidably connected to the inner wall of the sliding hole. A lead screw is threadedly penetrated through the side surface of the slider. One end of the output shaft of the servo motor is fixedly connected to one end of the lead screw. A connecting plate is fixedly connected to the upper surface of the slider. Fixed seats are fixedly connected to both sides of the connecting plate. A transmission wheel is rotatably connected to the inner wall of the fixed seat. A motor seat is fixedly connected to one side of one of the fixed seats. A driving motor is fixedly connected to the inner wall of the motor seat. One end of the output shaft of the driving motor is fixedly connected to one end of one of the transmission wheels. A conveyor belt is in driving connection with the arc surfaces of the two transmission wheels. The upper surface of the detection dish is in contact with the conveyor belt. Circular holes are formed on the surface of the conveyor belt. A cylinder is fixedly connected to the inner wall of the circular hole. The automatic pipette is located inside the cylinder.

[0012] Preferably, a positioning plate is fixedly connected to the outer wall of the cylinder. A positioning rod is slidably penetrated through the side surface of the positioning plate. Fixing plates are fixedly connected to both ends of the positioning rod. The two fixing plates are respectively fixedly connected to the mutually remote sides of the two fixed seats.

[0013] Preferably, a plurality of rectangular blocks are fixedly connected to the arc surface of the automatic pipette. A plurality of rectangular holes are formed at the upper end of the cylinder. The rectangular blocks are in contact with the inner walls of the rectangular holes.

[0014] Preferably, a guiding block is fixedly connected to the side of the detection dish away from the driving plate. The guiding block is provided in a wedge shape.

[0015] Preferably, an automatic conveying mechanism is provided on the side of the machine body away from the support plate. The automatic conveying mechanism includes a welding plate and a bearing plate. One side of the welding plate is fixedly connected to the machine body. A driving roller and a driven roller are rotatably connected to the side of the welding plate away from the machine body. A limiting plate is fixedly connected to the side of the welding plate away from the machine body. A motor is fixedly connected to one side of the limiting plate. The output end of the motor is fixedly connected to the driving roller. A belt is in driving connection with the arc surfaces of the driving roller and the driven roller. A threaded rod is rotatably inserted through the side surface of the welding plate. One end of the threaded rod is fixedly connected to the driven roller. An ear plate is threadedly connected to the arc surface of the threaded rod. A conveying frame is fixedly connected to the upper surface of the ear plate. The cross-section of the conveying frame is "C" shaped. One side of the bearing plate is in contact with the driving plate. The upper surface of the bearing plate and the upper surface of the driving plate are in the same plane. An auxiliary plate is fixedly connected to the lower surface of the bearing plate. One side of the auxiliary plate is fixedly connected to the side of the support plate close to the driving plate.

[0016] Preferably, a guide rod is slidably inserted through one side of the conveying frame. One end of the short arm of the guide rod is fixedly connected to the welding plate.

[0017] Preferably, a first limiting hole is formed in the upper surface of the bearing plate, and a second limiting hole is formed in the upper surface of the driving plate. The first limiting hole and the second limiting hole are communicated with each other. A limiting block is slidably connected to the inner wall of the first limiting hole. The limiting block is fixedly connected to the lower surface of the test dish.

[0018] Preferably, a sealing mechanism is provided on the side of the machine body close to the detection hole. The sealing mechanism includes a rotating seat. One side of the rotating seat is fixedly connected to the machine body. A rotating shaft is rotatably inserted through the inner wall of the rotating seat. A connecting plate is fixedly connected to the arc surface of the rotating shaft. A sealing plate is fixedly connected to one side of the connecting plate. An auxiliary hole is formed in the side surface of the sealing plate. An arc-shaped hole is formed in one side of the rotating seat. The center of the arc-shaped hole is coaxial with the axis of rotation of the rotating shaft. A fixing rod is slidably connected to the inner wall of the arc-shaped hole. One end of the fixing rod is fixedly connected to one side of the connecting plate. An adjusting ring is threadedly connected to the arc surface of the fixing rod.

[0019] Preferably, a rubber pad is fixedly connected to the side of the adjusting ring close to the rotating seat. The diameter of the rubber pad is larger than the diameter of the adjusting ring.

[0020] Preferably, a coil spring is sleeved on the arc surface of the rotating shaft. The two ends of the coil spring are respectively fixedly connected to the connecting plate and the rotating seat.

[0021] The present invention also discloses a microbial fluorescence detection method for continuing detection using the above microbial fluorescence detection system, including the steps:

[0022] 1), Use the automatic pipette to transfer 50 µL of the sample to be detected into each detection cavity, and then place the detection dish into the microbial fluorescence detector;

[0023] 2), Select the detection conditions and obtain the detection results in real time. The time for obtaining the detection results does not exceed 27 minutes.

[0024] Compared with the prior art, the advantages and positive effects of the present invention are as follows.

[0025] 1. In the present invention, two effects are achieved by setting the automatic pipetting mechanism: First, it can automatically control the pipette to drop the liquid to be detected into multiple detection cavities of the detection dish. The automatic dropping process makes the experimental process more coherent and systematic, reduces the operation steps and time waste, improves the detection efficiency, and reduces the error caused by human operation, thus enhancing the reliability of the experimental results; Second, during the dropping process, the conveyor belt can seal and block the detection cavity, thereby avoiding the splashing of the detection liquid during the dropping process, thus facilitating the influence of sample loss and avoiding environmental pollution of the experiment.

[0026] 2. In the present invention, by setting the automatic conveying mechanism, the detection dish can be automatically conveyed from the driving plate to the automatic dropping mechanism, and after the dropping operation is completed, the detection dish can be automatically conveyed from the automatic dropping mechanism to the driving plate, further improving the automation degree of the microbial fluorescence detection system and further improving the detection efficiency.

[0027] 3. In the present invention, by setting the sealing mechanism, after the driving plate drives the detection dish to move into the body, the operator can conveniently adjust the sealing plate to block the detection hole, thereby sealing the detection hole, and further improving the practicality of the microbial fluorescence detection system.

[0028] 4. The detection results are completed in less than half an hour, shortening the product release time by two to five days. Therefore, it can reduce the storage space and cost, with faster inventory turnover, greater flexibility and responsiveness, and meet customer needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic three-dimensional structure diagram of a microbial fluorescence detection system proposed by the present invention;

[0030] Figure 2 It is a schematic structure diagram of the automatic dropping mechanism of a microbial fluorescence detection system proposed by the present invention;

[0031] Figure 3 It is a partial schematic diagram of the automatic dropping mechanism of a microbial fluorescence detection system proposed by the present invention;

[0032] Figure 4The present invention proposes a partial structural disassembly schematic diagram of an automatic dripping mechanism of a microbial fluorescence detection system;

[0033] Figure 5 The present invention provides a structural schematic diagram of a detection dish of a microbial fluorescence detection system;

[0034] Figure 6 The present invention provides a schematic diagram of the structure of an automatic pipette gun for a microbial fluorescence detection system;

[0035] Figure 7 The present invention proposes a structural schematic diagram of an automatic conveying mechanism of a microbial fluorescence detection system;

[0036] Figure 8 A partial structural schematic diagram of an automatic conveying mechanism of a microbial fluorescence detection system proposed by the present invention;

[0037] Figure 9 The structural schematic diagram of a carrier plate of a microbial fluorescence detection system proposed by the present invention;

[0038] Figure 10 The present invention provides a structural schematic diagram of a sealing mechanism of a microbial fluorescence detection system;

[0039] Figure 11 This is a partial structural diagram of a sealing mechanism of a microbial fluorescence detection system proposed by the present invention.

[0040] Legend: 1. Machine body; 2. Detection hole; 3. Automatic dripping mechanism; 301. Support plate; 302. Servo motor; 303. Slide hole; 304. Screw rod; 305. Sliding block; 306. Connecting plate; 307. Fixed seat; 308. Transmission wheel; 309. Motor seat; 310. Driving motor; 311. Conveyor belt; 312. Round hole; 313. Cylinder; 314. Automatic pipette gun; 315. Positioning plate; 316. Positioning rod; 317. Fixed plate; 318. Rectangular hole; 319. Rectangular block; 320. Detection dish; 321. Detection cavity; 322. Guide block; 4. Automatic conveying mechanism; 401. Welding plate; 402, limit plate; 403, motor; 404, active reel; 405, driven reel; 406, belt; 407, threaded rod; 408, ear plate; 409, conveyor frame; 410, guide rod; 411, bearing plate; 412, auxiliary plate; 413, first limit hole; 414, second limit hole; 415, limit block; 5, sealing mechanism; 501, rotating seat; 502, rotating shaft; 503, connecting plate; 504, sealing plate; 505, auxiliary hole; 506, coil spring; 507, arc hole; 508, fixing rod; 509, adjusting ring; 510, rubber pad; 6, driving plate; 7, mounting plate; 8, bottle holding; 9, catheter. Detailed implementation mode

[0041] In order to more clearly understand the above-mentioned objects, features and advantages of the present invention, the present invention will be further described below in conjunction with the drawings and embodiments. It should be noted that, without conflict, the embodiments of the present application and the features in the embodiments can be combined with each other.

[0042] In the following description, many specific details are set forth in order to fully understand the present invention. However, the present invention can also be implemented in other ways different from those described herein. Therefore, the present invention is not limited by the specific embodiments disclosed in the following specification.

[0043] Embodiment 1, as Figure 1 shown, the present invention provides a microbial fluorescence detection system, including a body 1, a detection hole 2, a driving plate 6, a mounting plate 7, a storage bottle 8, a conduit 9 and an automatic liquid dropping mechanism 3. A driving device for automatically moving the driving plate 6 in and out of the detection hole 2 is installed inside the body 1. The storage bottle 8 is used to store the fluorescent dye. The conduit 9 is used to transport the fluorescent dye from the storage bottle 8 to the test dish 320, and the transportation process is controlled by a delivery pump inside the body 1. The detection hole 2 is provided on one side of the body 1, the driving plate 6 is installed inside the detection hole 2, the mounting plate 7 is installed on the body 1, and a plurality of storage bottles 8 are installed on one side of the mounting plate 7. One end of the conduit 9 is communicated with the storage bottle 8.

[0044] Among them, the automatic liquid dropping mechanism 3 is located on one side of the body 1, an automatic conveying mechanism 4 is provided on the side of the body 1 away from the support plate 301, and a sealing mechanism 5 is provided on the side of the body 1 close to the detection hole 2.

[0045] A central control system is also installed inside the body 1 to control the automatic liquid dropping mechanism 3 and the automatic conveying mechanism 4. The process and principle of the central control system receiving and sending signals are similar to those of common existing control systems, and are not the key points to be protected by the present invention, so no detailed description will be given.

[0046] Next, the specific settings and functions of its automatic liquid dropping mechanism 3, automatic conveying mechanism 4 and sealing mechanism 5 will be specifically described.

[0047] As Figures 2 to 6 shown, the automatic liquid dropping mechanism 3 includes a support plate 301, a test dish 320 and an automatic pipette 314. A plurality of detection cavities 321 for storing the liquid to be detected are formed on the upper surface of the test dish 320, and the detection cavities 321 are evenly distributed on the upper surface of the test dish 320. The lower surface of the test dish 320 is in the same plane as the upper surface of the driving plate 6, which can prevent the test dish 320 from getting stuck during transportation. One side of the support plate 301 is fixedly connected to the body 1, and the support plate 301 plays a supporting role.

[0048] On one side of the support plate 301, a servo motor 302 is fixedly connected. A sliding hole 303 is formed in the upper surface of the support plate 301. A slider 305 is slidably connected to the inner wall of the sliding hole 303. A lead screw 304 is threadedly passed through the side surface of the slider 305. The output end of the servo motor 302 is fixedly connected to one end of the lead screw 304. The upper surface of the slider 305 is fixedly connected to a connecting plate 306. Starting the servo motor 302 drives the lead screw 304 to rotate. The lead screw 304 drives the slider 305, causing the slider 305 to move along the inner wall of the sliding hole 303. The slider 305 drives the connecting plate 306, and the connecting plate 306 drives the automatic pipette 314 above it, enabling the automatic pipette 314 to move horizontally automatically.

[0049] On both sides of the connecting plate 306, fixed seats 307 are fixedly connected. A driving wheel 308 is rotatably connected to the inner wall of the fixed seat 307. On one side of one of the fixed seats 307, a motor seat 309 is fixedly connected. A driving motor 310 is fixedly connected to the inner wall of the motor seat 309. The output end of the driving motor 310 is fixedly connected to one end of one of the driving wheels 308. The arc surfaces of the two driving wheels 308 are drivingly connected by a conveyor belt 311. The upper surface of the test dish 320 is in contact with the conveyor belt 311, which can prevent liquid from splashing during the liquid dripping process. Circular holes 312 are formed in the surface of the conveyor belt 311. A cylinder 313 is fixedly connected to the inner wall of the circular hole 312. The automatic pipette 314 is located inside the cylinder 313. Starting the driving motor 310 drives the driving wheel 308 to rotate. The driving wheel 308 drives the conveyor belt 311 to operate. The conveyor belt 311 drives the cylinder 313, and the cylinder 313 drives the automatic pipette 314 to move, enabling the automatic pipette 314 to move longitudinally automatically.

[0050] In summary, the longitudinal movement and the horizontal movement of the automatic pipette 314 cooperate with each other, enabling the automatic pipette 314 to automatically adjust the addition of the liquid to be tested into each test cavity 321 on the test dish 320.

[0051] A positioning plate 315 is fixedly connected to the outer wall of the cylinder 313. A positioning rod 316 is slidably passed through the side surface of the positioning plate 315. Fixing plates 317 are fixedly connected to both ends of the positioning rod 316. The two fixing plates 317 are respectively fixedly connected to the sides of the two fixed seats 307 away from each other. When the conveyor belt 311 drives the cylinder 313 and the automatic pipette 314 to move, the positioning plate 315 fixed on the cylinder 313 will move along the arc surface of the positioning rod 316, thereby improving the stability of the process of the cylinder 313 driving the automatic pipette 314 to move, enhancing the stability of the liquid dripping process, and preventing the center of gravity of the automatic pipette 314 and the cylinder 313 from being too high and causing shaking.

[0052] The arc surface of the automatic pipette 314 is fixedly connected with a plurality of rectangular blocks 319. A plurality of rectangular holes 318 are opened at the upper end of the cylinder 313, and the rectangular blocks 319 are attached to the inner walls of the rectangular holes 318. Before the liquid dripping operation, the rectangular blocks 319 fixed on the automatic pipette 314 can be placed into the rectangular holes 318, so that the automatic pipette 314 can be quickly supported and positioned.

[0053] A guide block 322 is fixedly connected to the side of the test dish 320 away from the drive plate 6. The guide block 322 is arranged in a wedge shape. By arranging the guide block 322 in a wedge shape on one side of the test dish 320, the process of moving the test dish 320 into the conveyor belt 311 can be made smoother, and the conveyor belt can be prevented from jamming the test dish 320 and making it immovable.

[0054] The whole automatic liquid dripping mechanism 3 achieves the following effects. By setting up the automatic liquid transfer mechanism, two effects are achieved: First, it can automatically control the pipette to drip the liquid to be tested into multiple test cavities 321 of the test dish 320. The automatic dripping process makes the experimental process more coherent and systematic, reduces the operation steps and time waste, improves the detection efficiency, and reduces the errors caused by manual operation, thus enhancing the reliability of the experimental results. Second, during the liquid dripping process, the conveyor belt 311 can hermetically shield the test cavities 321, thereby preventing the test liquid from splashing during the dripping process, avoiding the influence of sample loss, and preventing environmental pollution of the experiment.

[0055] As Figures 7 to 9 shown, the automatic conveying mechanism 4 includes a welding plate 401 and a bearing plate 411. One side of the welding plate 401 is fixedly connected to the machine body 1. A driving roller 404 and a driven roller 405 are rotatably connected to the side of the welding plate 401 away from the machine body 1. A limiting plate 402 is fixedly connected to the side of the welding plate 401 away from the machine body 1. A motor 403 is fixedly connected to one side of the limiting plate 402. The output end of the motor 403 is fixedly connected to the driving roller 404. A belt 406 is in driving connection with the arc surfaces of the driving roller 404 and the driven roller 405. A threaded rod 407 is rotatably inserted through the side surface of the welding plate 401. One end of the threaded rod 407 is fixedly connected to the driven roller 405. An ear plate 408 is threadedly connected to the arc surface of the threaded rod 407. A conveying frame 409 is fixedly connected to the upper surface of the ear plate 408. The cross section of the conveying frame 409 is in the shape of "C". Starting the motor 403 drives the driving roller 404 to rotate. The driving roller 404 drives the driven roller 405 to rotate through the belt 406. The driven roller 405 drives the threaded rod 407 to rotate. The threaded rod 407 drives the conveying frame 409 to move, so as to automatically push the test dish 320 by using the conveying frame 409.

[0056] One side of the bearing plate 411 is in contact with the driving plate 6. The upper surface of the bearing plate 411 and the upper surface of the driving plate 6 are in the same plane. A supporting plate 412 is fixedly connected to the lower surface of the bearing plate 411. One side of the supporting plate 412 is fixedly connected to one side of the supporting plate 301 close to the driving plate 6. During the pushing process of the test dish 320, the bearing plate 411 can always support the test dish 320.

[0057] In summary, when it is necessary to automatically push the test dish 320 from the driving plate 6 to the position to be dripped, first start the motor 403 to drive the active roller 404 to rotate. The active roller 404 drives the driven roller 405 to rotate by means of the belt 406. The driven roller 405 drives the threaded rod 407 to rotate. The threaded rod 407 drives the conveying frame 409 to move. The conveying frame 409 will push the test dish 320 on the driving plate 6, so that the test dish 320 moves along the surfaces of the driving plate 6 and the bearing plate 411 in sequence to the inside of the conveyor belt 311. Then the dripping operation can be carried out. When the dripping operation is completed, start the motor 403 to drive the active roller 404 to rotate in the reverse direction. The active roller 404 drives the driven roller 405 to rotate by means of the belt 406. The driven roller 405 drives the threaded rod 407 to rotate. The threaded rod 407 drives the conveying frame 409 to move. The conveying frame 409 will push the test dish 320 on the bearing plate 411, so that the test dish 320 moves from the surface of the bearing plate 411 to the driving plate 6.

[0058] A guide rod 410 is slidably inserted through one side of the conveying frame 409. One end of the short arm of the guide rod 410 is fixedly connected to the welding plate 401. When the threaded rod 407 drives the conveying frame 409 to move, the guide rod 410 can guide and limit the conveying frame 409, so as to avoid the deviation of the conveying frame 409 during the moving process.

[0059] A first limiting hole 413 is formed in the upper surface of the bearing plate 411. A second limiting hole 414 is formed in the upper surface of the driving plate 6. The first limiting hole 413 is communicated with the second limiting hole 414. A limiting block 415 is slidably connected to the inner wall of the first limiting hole 413. The limiting block 415 is fixedly connected to the lower surface of the test dish 320. When the conveying frame 409 pushes the test dish 320 to move along the upper surface of the bearing plate 411 or the driving plate 6, the limiting block 415 fixed on the test dish 320 will move along the inner walls of the first limiting hole 413 and the second limiting hole 414, so as to avoid the deviation of the test dish 320 during the moving process.

[0060] The effect achieved by the entire automatic conveying mechanism 4 is that by setting up the automatic conveying mechanism 4, the test dish 320 can be automatically conveyed from the driving plate 6 to the automatic liquid dropping mechanism 3, and after the liquid dropping operation is completed, the test dish 320 can be automatically conveyed from the automatic liquid dropping mechanism 3 to the driving plate 6, further improving the automation degree of the microbial fluorescence detection system and further improving the detection efficiency.

[0061] As Figure 10 and Figure 11 shown, the sealing mechanism 5 includes a rotating seat 501. One side of the rotating seat 501 is fixedly connected to the machine body 1. A rotating shaft 502 is rotatably penetrated through the inner wall of the rotating seat 501. A connecting plate 503 is fixedly connected to the arc surface of the rotating shaft 502. A sealing plate 504 is fixedly connected to one side of the connecting plate 503. An auxiliary hole 505 is formed on the side surface of the sealing plate 504. An arc-shaped hole 507 is formed on one side of the rotating seat 501. The center of the arc-shaped hole 507 is coaxial with the axis of rotation of the rotating shaft 502. A fixing rod 508 is slidably connected to the inner wall of the arc-shaped hole 507. One end of the fixing rod 508 is fixedly connected to one side of the connecting plate 503. An adjusting ring 509 is threadedly connected to the arc surface of the fixing rod 508. When the sealing plate 504 is needed to block the detection hole 2 during the detection process, first pull the auxiliary hole 505 to drive the sealing plate 504. At this time, the sealing plate 504 will drive the connecting plate 503, so that the connecting plate 503 drives the rotating shaft 502 to rotate along the inner wall of the rotating seat 501. At the same time, the fixing rod 508 on the connecting plate 503 will slide along the inner wall of the arc-shaped hole 507. After the sealing plate 504 blocks the detection hole 2, then rotate the adjusting ring 509 to abut against the rotating seat 501, and the sealing operation of the detection hole 2 can be completed.

[0062] A rubber pad 510 is fixedly connected to the side of the adjusting ring 509 close to the rotating seat 501. The diameter of the rubber pad 510 is larger than the diameter of the adjusting ring 509. The rubber pad 510 can increase the friction force on the side of the adjusting ring 509 close to the rotating seat 501, achieving the effect that the adjusting ring 509 can abut against the rotating seat 501 stably with the help of the rubber pad 510.

[0063] A coil spring 506 is sleeved on the arc surface of the rotating shaft 502. The two ends of the coil spring 506 are respectively fixedly connected to the connecting plate 503 and the rotating seat 501. After the rotating adjusting ring 509 is separated from the rotating seat 501, the coil spring 506 can automatically drive the sealing plate 504 to rotate away from the detection hole 2, achieving the effect of being able to quickly open the sealing plate 504.

[0064] The effect achieved by the entire sealing mechanism 5 is that by setting up the sealing mechanism 5, after the driving plate 6 drives the test dish 320 to move into the machine body 1, the operator can conveniently adjust the sealing plate 504 to block the detection hole 2, so that the detection hole 2 can be sealed, thereby further improving the practicability of the microbial fluorescence detection system.

[0065] The overall working principle is that when the detection system is needed to detect or quantify microorganisms, the following steps need to be carried out:

[0066] S1. First, drive the plate 6 to move from the inside of the detection hole 2. When the second limit hole 414 on the drive plate 6 is aligned with the first limit hole 413 on the carrier plate 411, stop the movement of the drive plate 6. Then start the motor 403 to drive the active roller 404 to rotate. The active roller 404 drives the driven roller 405 to rotate through the belt 406. The driven roller 405 drives the threaded rod 407 to rotate. The threaded rod 407 drives the conveying frame 409 to move. The conveying frame 409 will push the test dish 320 on the drive plate 6, so that the test dish 320 moves along the surfaces of the drive plate 6 and the carrier plate 411 in sequence to the inside of the conveyor belt 311, so that the test dish 320 can be automatically pushed from the drive plate 6 to the liquid dropping position.

[0067] S2. When the test dish 320 is automatically pushed from the drive plate 6 to the liquid dropping position, start the servo motor 302 to drive the lead screw 304 to rotate. The lead screw 304 drives the slider 305, so that the slider 305 moves along the inner wall of the sliding hole 303. The slider 305 drives the connecting plate 306, and the connecting plate 306 drives the automatic pipette 314 above it, so that the automatic pipette 314 can move horizontally automatically. Start the drive motor 310 to drive the transmission wheel 308 to rotate. The transmission wheel 308 drives the conveyor belt 311 to operate. The conveyor belt 311 drives the cylinder 313, and the cylinder 313 drives the automatic pipette 314 to move, so that the automatic pipette 314 can move vertically automatically. The vertical movement and the horizontal movement of the automatic pipette 314 cooperate with each other, and 50 μl of the liquid to be detected can be automatically dropped into each detection cavity 321 on the test dish 320.

[0068] S3. After the liquid dropping operation is completed, start the motor 403 to drive the active roller 404 to rotate in the reverse direction. The active roller 404 drives the driven roller 405 to rotate through the belt 406. The driven roller 405 drives the threaded rod 407 to rotate. The threaded rod 407 drives the conveying frame 409 to move. The conveying frame 409 will push the test dish 320 on the carrier plate 411, so that the test dish 320 moves from the surface of the carrier plate 411 to the drive plate 6.

[0069] S4. When the test dish 320 moves from the surface of the carrier plate 411 to the drive plate 6, then control the drive plate 6 to drive the test dish 320 into the body 1. Start the transfer pump inside the body 1 to introduce the fluorescent dye into the test, so that the detection operation can be carried out, and the detection can be completed within 27 minutes.

[0070] The above are only the preferred embodiments of the present invention, and are not intended to limit the present invention in other forms. Any person skilled in the art may use the technical content disclosed above to make changes or modifications into equivalent embodiments with equivalent changes and apply them to other fields. However, as long as they do not depart from the technical solution content of the present invention, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention still fall within the protection scope of the technical solution of the present invention.

Claims

1. A microbial fluorescence detection system, comprising a body (1), a detection hole (2), a drive plate (6), a mounting plate (7), a containing bottle (8), a conduit (9) and an automatic dripping mechanism (3), characterized in that: The detection hole (2) is arranged on one side of the machine body (1); the drive plate (6) is installed inside the detection hole (2); the mounting plate (7) is installed on the machine body (1); a plurality of containing bottles (8) are installed on one side of the mounting plate (7); one end of the guide tube (9) is connected to the containing bottles (8); the automatic dripping mechanism (3) is located on one side of the machine body (1); the automatic dripping mechanism (3) comprises a support plate (301), a detection dish (320) and an automatic liquid transfer gun (314); the upper surface of the detection dish (320) A plurality of detection cavities (321) are provided on the surface of the detection dish (320), the detection cavities (321) are evenly distributed on the upper surface of the detection dish (320), the lower surface of the detection dish (320) and the upper surface of the driving plate (6) are located in the same plane, one side of the support plate (301) is fixedly connected to the body (1), one side of the support plate (301) is fixedly connected to a servo motor (302), the upper surface of the support plate (301) is provided with a sliding hole (303), the inner wall of the sliding hole (303) is slidably connected to a slider (305), and the A screw rod (304) is threadedly penetrated on the side surface of the slider (305); the output end of the servo motor (302) is fixedly connected to one end of the screw rod (304); a connecting plate (306) is fixedly connected to the upper surface of the slider (305); both sides of the connecting plate (306) are fixedly connected to fixing seats (307); the inner wall of the fixing seat (307) is rotatably connected to a transmission wheel (308); one side of the fixing seat (307) is fixedly connected to a motor seat (309); the inner wall of the motor seat (309) is fixedly connected to the inner wall of the fixing seat (307); A driving motor (310) is fixedly connected, the output end of the driving motor (310) is fixedly connected to one end of one of the transmission wheels (308), the arc surfaces of the two transmission wheels (308) are transmission-connected to a conveyor belt (311), the upper surface of the detection dish (320) is in contact with the conveyor belt (311), a circular hole (312) is provided on the surface of the conveyor belt (311), a cylinder (313) is fixedly connected to the inner wall of the circular hole (312), and the automatic liquid transfer gun (314) is located inside the cylinder (313).

2. A microbial fluorescence detection system according to claim 1, characterized in that: A positioning plate (315) is fixedly connected to the outer wall of the cylinder (313), a positioning rod (316) is slidably penetrated through the side surface of the positioning plate (315), both ends of the positioning rod (316) are fixedly connected to fixing plates (317), and the two fixing plates (317) are respectively fixedly connected to the sides of the two fixing seats (307) that are away from each other.

3. A microbial fluorescence detection system according to claim 1, characterized in that: A plurality of rectangular blocks (319) are fixedly connected to the arc surface of the automatic liquid transfer gun (314); a plurality of rectangular holes (318) are opened at the upper end of the cylinder (313); and the rectangular blocks (319) fit in contact with the inner walls of the rectangular holes (318).

4. A microbial fluorescence detection system according to claim 1, characterized in that: A guide block (322) is fixedly connected to a side of the detection dish (320) away from the drive plate (6), and the guide block (322) is configured as a wedge-shaped structure.

5. A microbial fluorescence detection system according to claim 1, characterized in that: An automatic conveying mechanism (4) is provided on a side of the machine body (1) away from the support plate (301), the automatic conveying mechanism (4) comprising a welding plate (401) and a bearing plate (411), one side of the welding plate (401) being fixedly connected to the machine body (1), the side of the welding plate (401) away from the machine body (1) being rotatably connected to a driving reel (404) and a driven reel (405), the side of the welding plate (401) away from the machine body (1) being fixedly connected to a limiting plate (402), one side of the limiting plate (402) being fixedly connected to a motor (403), the output end of the motor (403) being fixedly connected to the driving reel (404), the arc surface of the driving reel (404) and the driven reel (405) being transmission-connected by a belt ( 406), a threaded rod (407) is rotatably penetrated on the side of the welding plate (401), one end of the threaded rod (407) is fixedly connected to the driven reel (405), the arc surface of the threaded rod (407) is threadedly connected to an ear plate (408), the upper surface of the ear plate (408) is fixedly connected to a conveying frame (409), the cross-section of the conveying frame (409) is "C" shaped, one side of the bearing plate (411) is in contact with the driving plate (6), the upper surface of the bearing plate (411) and the upper surface of the driving plate (6) are located in the same plane, the lower surface of the bearing plate (411) is fixedly connected to an auxiliary plate (412), and one side of the auxiliary plate (412) is fixedly connected to a side of the support plate (301) close to the driving plate (6).

6. A microbial fluorescence detection system according to claim 5, characterized in that: A guide rod (410) is slidably provided on one side of the conveying frame (409), and one end of the short arm end of the guide rod (410) is fixedly connected to the welding plate (401).

7. A microbial fluorescence detection system according to claim 5, characterized in that: A first limiting hole (413) is formed on the upper surface of the bearing plate (411), a second limiting hole (414) is formed on the upper surface of the driving plate (6), the first limiting hole (413) is connected to the second limiting hole (414), an inner wall of the first limiting hole (413) is slidably connected to a limiting block (415), and the limiting block (415) is fixedly connected to the lower surface of the detection dish (320).

8. A microbial fluorescence detection system according to claim 1, characterized in that: A sealing mechanism (5) is provided on one side of the machine body (1) close to the detection hole (2), the sealing mechanism (5) comprising a rotating seat (501), one side of the rotating seat (501) being fixedly connected to the machine body (1), a rotating shaft (502) being rotatably penetrated through the inner wall of the rotating seat (501), a connecting plate (503) being fixedly connected to the circular arc surface of the rotating shaft (502), a sealing plate (504) being fixedly connected to one side of the connecting plate (503), and the sealing plate (504) An auxiliary hole (505) is provided on the side of the rotating seat (504), an arc hole (507) is provided on one side of the rotating seat (501), the center of the arc hole (507) is coaxial with the axis of rotation of the rotating shaft (502), a fixing rod (508) is slidably connected to the inner wall of the arc hole (507), one end of the fixing rod (508) is fixedly connected to one side of the connecting plate (503), and an adjusting ring (509) is threadedly connected to the arc surface of the fixing rod (508), A rubber pad (510) is fixedly connected to one side of the adjusting ring (509) close to the rotating seat (501); the diameter of the rubber pad (510) is greater than the diameter of the adjusting ring (509).

9. A microbial fluorescence detection system according to claim 8, characterized in that: The arc surface of the rotating shaft (502) is sleeved with a coil spring (506), and the two ends of the coil spring (506) are respectively fixedly connected to the connecting plate (503) and the rotating seat (501).

10. A microbial fluorescence detection method, according to the microbial fluorescence detection system according to any one of claims 1 to 8, characterized in that: Includes steps: 1) Use the automatic pipette to transfer 50 μL of the pre-test sample into each test cavity, and then place the test dish into the microbial fluorescence test; 2) Select the test conditions and obtain the test results in real time. The test results can be obtained within 27 minutes.

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