Method for simultaneously determining contents of various components in Mongolian medicine swelling-eliminating nine-ingredient powder

Abstract

The invention discloses a method for simultaneously determining the contents of various components in Mongolian medicine swelling-eliminating nine-ingredient powder. The method comprises the following steps: preparing a reference solution from five chemical components, namely bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol; preparing a test solution from Mongolian medicine detumescence nine-ingredient powder; respectively taking the reference substance solution and the test substance solution for high performance liquid chromatography detection, and respectively calculating the contents of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol in the Mongolian medicine swelling-diminishing nine-ingredient powder by utilizing an external standard method to obtain the contents of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol in the Mongolian medicine swelling-diminishing nine-ingredient powder. The method disclosed by the invention has the beneficial effects that the method is simple and easy to operate, the contents of five index components, namely bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol, in the Mongolian medicine swelling-eliminating nine-ingredient powder can be simultaneously measured, and reference is provided for development and quality standard formulation of the traditional Mongolian medicine preparation swelling-eliminating nine-ingredient powder.

Description

Technical field:

[0001] The patent of this invention belongs to the field of quality control methods for Mongolian medicine preparations, and specifically relates to a method for simultaneously determining the content of multiple ingredients in the Mongolian medicine Xiaozhong Jiuwei Powder. Background technology:

[0002] The Mongolian medicine Xiaozhong Jiuwei Powder has the functions of reducing swelling, relieving pain, removing toxins and dispersing blood stasis. It is mainly used to treat mumps, mastitis, furuncle, cellulitis, lymphadenitis, subcutaneous and deep abscesses, erysipelas, nameless swelling and pain, traumatic injuries, rheumatic pain, etc. Its composition and preparation method are as described in the "Mongolian Medicine Chapter" of the 2014 edition of "National Medicine Formula Preparations". The specific formula is 100mg of Beijing Euphorbia, 200mg of Rhubarb, 100mg of Discoloration Herba Polygonati Odorati, 100mg of Curcuma Longa, 100mg of Acorus Calamus, 100mg of Raw Aconitum, 100mg of Asparagus Codonopsis. mg, rhubarb 100mg (drug content per gram); the ingredients of Mongolian medicine Xiaozhong Jiuwei Powder are complex, among which curcumin, demethoxycurcumin and bisdemethoxycurcumin are the effective indicator components of turmeric, and emodin and chrysophanol are the effective indicator components of rhubarb and rhubarb. Therefore, determining the content of each indicator component of curcumin, demethoxycurcumin, bisdemethoxycurcumin, emodin and chrysophanol in Mongolian medicine Xiaozhong Jiuwei Powder can better control the quality of Mongolian medicine Xiaozhong Jiuwei Powder.

[0003] At present, there is no literature report on a comprehensive analysis of the chemical components of the Mongolian medicine Xiaozhong Jiuwei Powder. In the existing technology, only one or two of the above five components in the Mongolian medicine are analyzed for quality. There is no comprehensive and systematic quality control method to reflect the quality status of the above five components in the Mongolian medicine Xiaozhong Jiuwei Powder, and it is impossible to effectively control its production process and product quality, and it is impossible to better guarantee its clinical efficacy. Summary of the invention:

[0004] In view of this, the object of the present invention is to provide a method for simultaneously determining the contents of multiple components in the Mongolian medicine Xiaozhong Jiuwei Powder. The method for simultaneously determining the contents of multiple components in the Mongolian medicine Xiaozhong Jiuwei Powder provided by the present invention is simple and easy to operate. The contents of various index components such as bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol in the Mongolian medicine Xiaozhong Jiuwei Powder can be simultaneously measured, which has positive significance for the formulation and development of quality standards for the traditional Mongolian medicine Xiaozhong Jiuwei Powder.

[0005] The technical solution of the present invention discloses a method for simultaneously determining the contents of multiple components in a Mongolian medicine detumescence nine-flavor powder, which comprises the following steps:

[0006] A reference solution was prepared by taking five chemical components including bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol;

[0007] Take Mongolian medicine Xiaozhong Jiuwei Powder to prepare the test solution;

[0008] The reference solution and the test solution were respectively subjected to HPLC detection, and then the contents of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol in the Mongolian medicine Xiaozhong Jiuwei Powder were calculated using the external standard method.

[0009] The chromatographic conditions for the high performance liquid chromatography detection are as follows: mobile phase A is acetonitrile, mobile phase B is 2% glacial acetic acid solution; isocratic elution: the ratio between mobile phase A and mobile phase B is 48:52; the flow rate is 1 mL / min, the column temperature is 35°C, the detection wavelength in the early stage 0 to 10 minutes is 420 to 424 nm, the detection wavelength in the later stage 10 to 35 minutes is 252 to 256 nm, and the injection volume is 10 μL.

[0010] Furthermore, the preparation method of the test solution includes the following steps: weighing 0.9 to 1.1 parts by weight of the Mongolian medicine detumescent nine-flavor powder and adding it to anhydrous methanol, ultrasonic extraction, diluting the volume to 25 parts by volume, filtering, and preparing the test solution; wherein the ratio between parts by weight and parts by volume is g:mL.

[0011] Furthermore, the power of ultrasonic extraction is 200-400W, the frequency is 40-60kHz, the extraction time is 30-60min, and the filtration adopts a 0.22μm or 0.45μm filter membrane.

[0012] Furthermore, the chromatographic column used was filled with octadecylsilane bonded silica gel, and the chromatographic column parameters were 250 mm×4.6 mm, 5 μm.

[0013] The technical solution of the present invention further discloses a method for simultaneously determining the contents of multiple components in the Mongolian medicine Xiaozhong Jiuwei Powder, which comprises the following steps:

[0014] A reference solution was prepared by taking five chemical components including bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol;

[0015] The reference solution with different injection mass was respectively taken for high performance liquid chromatography detection, and the standard curve corresponding to the different chemical components was drawn according to the injection mass of the different chemical components and the peak area measured corresponding to the injection mass of the corresponding chemical components;

[0016] A test solution was prepared from Mongolian medicine Xiaozhong Jiuwei Powder; the test solution was subjected to high performance liquid chromatography, and the peak areas of the obtained corresponding chemical components were substituted into the corresponding standard curve to calculate the injection mass of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin, and chrysophanol in Mongolian medicine Xiaozhong Jiuwei Powder;

[0017] The chromatographic conditions for the high performance liquid chromatography detection are as follows: mobile phase A is acetonitrile, mobile phase B is 2% glacial acetic acid solution; isocratic elution: the ratio between mobile phase A and mobile phase B is 48:52; the flow rate is 1 mL / min, the column temperature is 35°C, the detection wavelength in the early stage 0 to 10 minutes is 420 to 424 nm, the detection wavelength in the later stage 10 to 35 minutes is 252 to 256 nm, and the injection volume is 10 μL.

[0018] Furthermore, the reference solution with different injection masses is achieved by taking the reference solution of the same concentration and different injection volumes, wherein the injection mass of bisdemethoxycurcumin is between 0.0116 and 0.87 μg, the injection mass of demethoxycurcumin is between 0.01224 and 0.918 μg, the injection mass of curcumin is between 0.02008 and 1.506 μg, the injection mass of emodin is between 0.01244 and 0.933 μg, and the injection mass of chrysophanol is between 0.01968 and 1.476 μg.

[0019] Furthermore, the standard curves corresponding to the different chemical components are as follows:

[0020] Bisdemethoxycurcumin Y=99.095X+0.2475,R 2 =0.9996;

[0021] Demethoxycurcumin Y=98.281X+0.0989,R 2 =0.9998;

[0022] Curcumin Y=91.497X-0.2128, R 2 =0.9999;

[0023] Rhein Y=40.426X-0.0176,R 2 =0.9999;

[0024] Chrysophanol Y = 51.734X - 0.0611, R 2 =0.9999.

[0025] Furthermore, the preparation method of the test solution includes the following steps: weighing 0.9 to 1.1 parts by weight of the Mongolian medicine detumescent nine-flavor powder and adding it to anhydrous methanol, ultrasonic extraction, diluting the volume to 25 parts by volume, filtering, and preparing the test solution; wherein the ratio between parts by weight and parts by volume is g:mL.

[0026] Furthermore, the power of ultrasonic extraction is 200-400W, the frequency is 40-60kHz, the extraction time is 30-60min, and the filtration adopts a 0.22μm or 0.45μm filter membrane.

[0027] Furthermore, the chromatographic column used was filled with octadecylsilane bonded silica gel, and the chromatographic column parameters were 250 mm×4.6 mm, 5 μm.

[0028] Advantages of the present invention:

[0029] 1. The present invention discloses a method for simultaneously determining the contents of multiple components in a Mongolian medicine detumescence nine-flavor powder. The method is simple and easy to operate. The contents of five index components, including bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin, and chrysophanol, in the Mongolian medicine detumescence nine-flavor powder can be simultaneously measured, thereby better controlling the quality of the Mongolian medicine detumescence nine-flavor powder.

[0030] 2. The present invention discloses a method for simultaneously determining the contents of multiple components in the Mongolian medicine Xiaozhong Jiuwei Powder. By adjusting the chromatographic conditions, the test solution is sampled once and the wavelength is switched. The detection wavelength for the first 0 to 10 minutes is 422 nm, which is used to determine curcumin, demethoxycurcumin and bisdemethoxycurcumin. The detection wavelength for the last 10 to 35 minutes is 254 nm, which is used to determine emodin and chrysophanol. The baseline is stable.

[0031] 3. The determination method of the present invention is simple and rapid to operate, has strong specificity, high precision, high accuracy, good repeatability, stability and linearity, and meets the requirements of relevant methodologies. Description of the drawings:

[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0033] Figure 1 The figure is a standard curve diagram of bisdemethoxycurcumin in Example 1 of the present invention.

[0034] Figure 2 It is the demethoxycurcumin standard curve diagram in Example 1 of the present invention.

[0035] Figure 3 This is a standard curve diagram of curcumin in Example 1 of the present invention.

[0036] Figure 4 This is the standard curve of emodin in Example 1 of the present invention.

[0037] Figure 5 This is a standard curve diagram of chrysophanol in Example 1 of the present invention.

[0038] Figure 6The chromatogram of the reference substance solution in Example 1 of the present invention is shown in FIG.

[0039] Figure 7 1 is a chromatogram of the test solution in Example 1 of the present invention.

[0040] Figure 8 This is the chromatogram of the negative control solution in the methodological validation of Example 1 of the present invention. Specific implementation method:

[0041] The present invention is further described in detail below by way of examples.

[0042] Example 1: A method for simultaneously determining the contents of multiple components in Mongolian medicine Xiaozhong Jiuwei Powder, comprising the following steps:

[0043] a1) Preparation of test solution and reference solution

[0044] Xiaozhong Jiuwei Powder (batch number: 20190543) was provided by the Inner Mongolia International Mongolian Medicine Hospital. Bisdemethoxycurcumin (batch number: DST191009-021), demethoxycurcumin (batch number: DST190910-030), curcumin (batch number: DST1910411-014), emodin (batch number: DST190610-030), and chrysophanol (batch number: DST191010-032) were purchased from Lemeitian Pharmaceuticals and Desit Biotechnology. Methanol was analytical grade and purchased from Tianjin Zhiyuan Chemical Reagent Co., Ltd.; acetonitrile was chromatographic grade and purchased from Thermo Fisher Scientific Inc.; and water was ultrapure.

[0045] a11) Preparation of reference solution

[0046] Accurately weigh 2.9 mg of bisdemethoxycurcumin, 3.06 mg of demethoxycurcumin, 5.02 mg of curcumin, 3.11 mg of emodin, and 4.92 mg of chrysophanol, place in a 50 mL volumetric flask, add methanol to dissolve, and dilute to the scale to obtain a mixed reference solution, wherein the concentration of bisdemethoxycurcumin is 0.058 mg / mL, the concentration of demethoxycurcumin is 0.0612 mg / mL, the concentration of curcumin is 0.1004 mg / mL, the concentration of emodin is 0.0622 mg / mL, and the concentration of chrysophanol is 0.0984 mg / mL.

[0047] a12) Preparation of test solution

[0048] Accurately weigh 1.0 g of Mongolian medicine Xiaozhong Jiuwei Powder, place it in a stoppered conical flask, accurately add 25 mL of anhydrous methanol, weigh the mass, extract it by ultrasound (power 200 W, frequency 40 kHz) for 30 min, cool it, weigh it again, add anhydrous methanol to make up for the lost mass, shake it well, filter it through a 0.45 μm filter membrane, and take the filtrate as the test solution.

[0049] a2) High performance liquid chromatography detection

[0050] a21) Chromatographic conditions

[0051] Chromatographic conditions: Thermo scientific C 18 Chromatographic column, 250 mm × 4.6 mm, 5 μm; mobile phase A is acetonitrile, mobile phase B is 2% glacial acetic acid solution; isocratic elution: the ratio between mobile phase A and mobile phase B is 48:52; the flow rate is 1 mL / min, the column temperature is 35°C, the detection wavelength from 0 to 10 min is 422 nm (for detecting bisdemethoxycurcumin, demethoxycurcumin, and curcumin), the detection wavelength from 10 to 35 min is 254 nm (for detecting emodin and chrysophanol), and the injection volume is 10 μL.

[0052] a22) Take the test solution and perform high performance liquid chromatography under the above chromatographic conditions to obtain the peak area A of the corresponding chemical components contained in the test solution. 供试品-相应化学成分 ; That is, the peak areas corresponding to bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol in the test solution are obtained respectively, that is, A 供试品-双去甲氧基姜黄素 、A 供试品-去甲氧基姜黄素 、A 供试品-姜黄素 、A 供试品-大黄素 、A 供试品-大黄酚 .

[0053] a3) Linear relationship investigation

[0054] Accurately pipette 0.2, 0.5, 1.0, 2.5, 5.0, 7.5, 10, and 15 μL of the reference solution prepared in step a11) and inject them into the chromatographic conditions of step a21). Perform a linear regression using the injected mass of the reference solution as the abscissa (X) and the peak area as the ordinate (Y).

[0055] The standard curves drawn using reference solutions of different injection masses are as follows:

[0056] Bisdemethoxycurcumin Y=99.095X+0.2475,R 2 =0.9996, standard curve see Figure 1 ;

[0057] Demethoxycurcumin Y=98.281X+0.0989,R 2 =0.9998, standard curve see Figure 2 ;

[0058] Curcumin Y=91.497X-0.2128, R 2 =0.9999, standard curve see Figure 3 ;

[0059] Rhein Y=40.426X-0.0176,R 2 =0.9999, standard curve see Figure 4 ;

[0060] Chrysophanol Y = 51.734X - 0.0611, R 2 =0.9999, standard curve see Figure 5 .

[0061] a4) taking the test solution and performing high performance liquid chromatography under the chromatographic conditions of step a21), respectively obtaining the peak areas corresponding to bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol contained in the test solution, substituting the peak areas of the corresponding chemical components into the corresponding standard curves, and obtaining the injection mass of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol in the test solution. The injection mass of each component is divided by the injection volume of the test solution to obtain the concentration of the corresponding chemical component in the test solution (C 供试品-相应化学成分 ).

[0062] Then the concentration of the corresponding chemical components in the test solution (C 供试品-相应化学成分 ) multiplied by the total volume of the prepared test solution (V 供试品 ) divided by the amount of Mongolian medicine Xiaozhong Jiuwei Powder used to prepare the test solution (m 蒙药消肿九味散 ), that is, the content of the corresponding chemical components in the Mongolian medicine Xiaozhong Jiuwei Powder, that is, the content of the corresponding chemical components in the Mongolian medicine Xiaozhong Jiuwei Powder = (C 供试品-相应化学成分 ×V 供试品 ) / m 蒙药消肿九味散 .

[0063] Taking bisdemethoxycurcumin as an example, the peak area (A) of bisdemethoxycurcumin measured by the test solution under the chromatographic conditions in Example 1 is 供试品-双去甲氧基姜黄素 ) was substituted into the standard curve corresponding to bisdemethoxycurcumin to obtain the injection mass of bisdemethoxycurcumin in the test solution. The injection mass of bisdemethoxycurcumin was divided by the injection volume of the test solution to obtain the concentration of bisdemethoxycurcumin in the test solution (C 供试品-双去甲氧基姜黄素 ).

[0064] The content of bisdemethoxycurcumin in Mongolian medicine Xiaozhong Jiuwei Powder = (C 供试品-双去甲氧基姜黄素 ×V 供试品 ) / m 蒙药消肿九味散 , and so on for the calculation methods of other chemical components.

[0065] Specifically, the test results of three equal samples in this example are shown in Table 1.

[0066] Table 1 Determination results of the content of each component in the sample (mg / g)

[0067]

[0068] Experimental Example 1 Methodological Investigation

[0069] This experimental example investigates the multi-component content determination method of the Mongolian medicine Xiaozhong Jiuwei Powder, mainly examining the specificity, precision, repeatability, stability and accuracy of the content determination method.

[0070] 1. Specificity

[0071] Preparation of negative control solution: Prepare a sample lacking turmeric, rhubarb and rhubarb according to the formula of Mongolian medicine Xiaozhong Jiuwei Powder, then prepare a negative control solution using the sample lacking turmeric, rhubarb and rhubarb according to the preparation method of the test solution in step a12) of Example 1.

[0072] According to the HPLC detection conditions in Example 1, 10 μL of the reference solution, the test solution and the negative control solution prepared in Example 1 were accurately aspirated and injected into the liquid chromatograph. The results were shown in Table 1. Figures 6-8 As shown, Figure 6 is the chromatogram of the reference solution. Figure 7 is the chromatogram of the test solution, Figure 8 is the chromatogram of the negative control solution.

[0073] from Figures 6-8 As can be seen from the results, the separation between the chromatographic peaks in the test sample and their adjacent peaks is greater than 1.5. The chromatographic peaks of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin, and chrysophanol are baseline-separated and have moderate retention times. The retention times and peak shapes of the chromatographic peaks of the reference substance for these five components are consistent with those of the test sample, with symmetrical peak shapes and no tailing. Furthermore, the chromatographic peaks of these five components do not appear in the chromatogram of the negative control solution under these chromatographic conditions. This indicates that there is no interference with the determination of other medicinal flavor pairs and that the specificity is good.

[0074] 2. Precision

[0075] Take the test solution (Inner Mongolia International Mongolian Medicine Hospital, batch number 20190543, sampling size 1.0017 g) in step a12) in Example 1, press the "a21" chromatographic condition in Example 1, continuously inject 6 times, the sample volume is 10 μL, and the RSDs of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol content are measured to be 0.53%, 0.47%, 0.58%, 1.11% and 0.80%, respectively, indicating that the instrument precision is good. The results are shown in Table 2.

[0076] Table 2 Precision test results (n=6)

[0077]

[0078] 3. Repeatability

[0079] Take the same batch of Xiaozhong Jiuwei Powder (Inner Mongolia International Mongolian Medicine Hospital, batch number 20190543), take 6 parts of the test solution in step a12) in Example 1, and measure the sample according to the chromatographic conditions of "a21" in Example 1. The injection volume is 10 μL, and the RSDs of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol are 2.17%, 1.33%, 0.55%, 2.91% and 1.02%, respectively, indicating that the method has good repeatability. The results are shown in Table 3.

[0080] Table 3 Repeatability test results of determination of content of index components of Xiaozhong Jiuwei Powder

[0081]

[0082] 4. Stability

[0083] The same batch of test solution (Inner Mongolia International Mongolian Medicine Hospital, batch number 20190543, sampling size is 1.0017 g) was taken and placed at room temperature for 0, 2, 4, 6, 8, 10, 12, and 24 h. The sample was injected according to the chromatographic condition of "a21" in Example 1, and the injection volume was 10 μL. The RSDs of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin, and chrysophanol were 2.91%, 1.37%, 0.23%, 2.29%, and 0.29%, respectively, indicating that the test solution had good stability within 24 h at room temperature. The results are shown in Table 4.

[0084] Table 4 Stability test results (mg / g)

[0085]

[0086] 5. Sample recovery test

[0087] Take about 0.5g of the known content of Xiaozhong Jiuweisan sample (Inner Mongolia International Mongolian Medicine Hospital, batch number 20190543), accurately weigh 6 parts, place in a stoppered conical flask, accurately add the mass concentration of 0.098mg / mL bisdemethoxycurcumin reference solution and the mass concentration of 0.088mg / mL demethoxycurcumin reference solution mixed solution 3mL, the mass concentration of 0.106mg / mL curcumin reference solution 11mL, the mass concentration of 0.068mg / mL emodin reference solution 3mL, 0.1208mg / mL chrysophanol 8mL prepared by the method under "a21" in Example 1, and measure the sample under the chromatographic conditions of "a21" in Example 1, with an injection volume of 10 μL, and the peak area is measured according to the law and the recovery value is calculated. The recovery range is 99.18~101.8%, which meets the relevant regulations. The results are shown in Table 5.

[0088] Table 5 Recovery results of 5 components in the sample (n=6)

[0089]

[0090]

[0091] The systematic methodological investigation showed that the specificity was strong and the linear relationship of the five selected compounds was good. 2 All were greater than 0.999; the RSD value of precision was 0.47~1.11%, the RSD value of repeatability was 0.55~2.91%; the average recovery rate was 99.18~101.8%, and the RSD value of stability was 0.23~2.91%. The results showed that this method has good precision, repeatability and stability, meets the requirements of relevant methodology, and can be used for sample content determination.

[0092] Example 2: The overall method is the same as that of Example 1, except that Xiaozhong Jiuwei Powder (batch number: 20180126) is provided by Inner Mongolia Kulun Mongolian Medicine Co., Ltd.

[0093] Example 3: The overall method is the same as that of Example 1, except that Xiaozhong Jiuwei Powder (batch number: 20180525) is provided by Inner Mongolia Kulun Mongolian Medicine Co., Ltd.

[0094] The results of the determination of the content of the Xiaozhong Jiuwei Powder of Example 2 and Example 3 are shown in Table 6.

[0095] Table 6 Desquamation Nine Flavors Powder Sample Content Determination Results

[0096]

[0097] The above is a preferred embodiment of the present invention. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be considered as the scope of protection of the present invention.

Claims

1. A method for simultaneously determining the contents of multiple components in Mongolian medicine Xiaozhong Jiuwei Powder, characterized in that: It includes the following steps: A total of five chemical components, including bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin, and chrysophanol, were used to prepare a reference solution; Take Mongolian medicine Xiaozhong Jiuwei Powder to prepare the test solution; The reference solution and the test solution were respectively taken for high performance liquid chromatography detection, and then the contents of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol in the Mongolian medicine Xiaozhong Jiuwei Powder were calculated by the external standard method. The chromatographic conditions of the high performance liquid chromatography detection are as follows: mobile phase A is acetonitrile, mobile phase B is 2% glacial acetic acid solution; isocratic elution: the ratio between mobile phase A and mobile phase B is 48:52; the flow rate is 1 mL / min, the column temperature is 35°C, the detection wavelength in the early stage 0 to 10 min is 420 to 424 nm, the detection wavelength in the later stage 10 to 35 min is 252 to 256 nm, and the injection volume is 10 μL.

2. The method for simultaneously determining the contents of multiple components in the Mongolian medicine detumescence Jiuwei powder according to claim 1, characterized in that: The preparation method of the test solution comprises the following steps: weighing 0.9 to 1.1 parts by weight of the Mongolian medicine detumescence nine-flavor powder and adding it to anhydrous methanol, ultrasonic extraction, fixing the volume to 25 parts by volume, filtering, and obtaining the test solution; wherein the ratio between parts by weight and parts by volume is g:mL.

3. A method for simultaneously determining the contents of multiple components in Mongolian medicine Xiaozhong Jiuwei Powder according to claim 2, characterized in that: The power of ultrasonic extraction is 200-400W, the frequency is 40-60kHz, the extraction time is 30-60min, and the filtration adopts 0.22μm or 0.45μm filter membrane.

4. The method for simultaneously determining the contents of multiple components in the Mongolian medicine detumescence Jiuwei powder according to claim 1, characterized in that: The chromatographic column used was filled with octadecylsilane bonded silica gel, and the column parameters were 250 mm × 4.6 mm, 5 μm.

5. A method for simultaneously determining the contents of multiple components in Mongolian medicine Xiaozhong Jiuwei Powder, characterized in that: It includes the following steps: A total of five chemical components, including bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin, and chrysophanol, were used to prepare a reference solution; The reference substance solutions of different injection masses are respectively taken for high performance liquid chromatography detection, and the standard curves corresponding to the different chemical components are drawn according to the injection masses of the different chemical components and the peak areas measured corresponding to the injection masses of the corresponding chemical components; A test solution was prepared by taking Mongolian medicine Xiaozhong Jiuwei Powder; the test solution was tested by high performance liquid chromatography, and the peak areas of the obtained corresponding chemical components were substituted into the corresponding standard curve to calculate the injection masses of bisdemethoxycurcumin, demethoxycurcumin, curcumin, emodin and chrysophanol in Mongolian medicine Xiaozhong Jiuwei Powder respectively; The chromatographic conditions of the high performance liquid chromatography detection are as follows: mobile phase A is acetonitrile, mobile phase B is 2% glacial acetic acid solution; isocratic elution: the ratio between mobile phase A and mobile phase B is 48:52; the flow rate is 1 mL / min, the column temperature is 35°C, the detection wavelength in the early stage 0 to 10 min is 420 to 424 nm, the detection wavelength in the later stage 10 to 35 min is 252 to 256 nm, and the injection volume is 10 μL.

6. A method for simultaneously determining the contents of multiple components in Mongolian medicine Xiaozhong Jiuwei Powder according to claim 5, characterized in that: The reference solution with different injection masses is realized by taking the reference solution with the same concentration and different injection volumes, wherein the injection mass of bisdemethoxycurcumin is between 0.0116 and 0.87 μg, the injection mass of demethoxycurcumin is between 0.01224 and 0.918 μg, the injection mass of curcumin is between 0.02008 and 1.506 μg, the injection mass of emodin is between 0.01244 and 0.933 μg, and the injection mass of chrysophanol is between 0.01968 and 1.476 μg.

7. The method for simultaneously determining the contents of multiple components in Mongolian medicine Xiaozhong Jiuwei Powder according to claim 5, characterized in that: The standard curves corresponding to the different chemical components are as follows: Bisdemethoxycurcumin Y = 99.095X + 0.2475, R 2 =0.9996; Demethoxycurcumin Y = 98.281X + 0.0989, R 2 =0.9998; Curcumin Y = 91.497X - 0.2128, R 2 =0.9999; Rheum officinale Y=40.426X-0.0176,R 2 =0.9999; Chrysophanol Y = 51.734X-0.0611, R 2 =0.9999.

8. The method for simultaneously determining the contents of multiple components in Mongolian medicine Xiaozhong Jiuwei Powder according to claim 5, characterized in that: The preparation method of the test solution comprises the following steps: weighing 0.9 to 1.1 parts by weight of the Mongolian medicine detumescence nine-flavor powder and adding it to anhydrous methanol, ultrasonic extraction, fixing the volume to 25 parts by volume, filtering, and obtaining the test solution; wherein the ratio between parts by weight and parts by volume is g:mL.

9. A method for simultaneously determining the contents of multiple components in Mongolian medicine Xiaozhong Jiuwei Powder according to claim 8, characterized in that: The power of ultrasonic extraction is 200-400W, the frequency is 40-60kHz, the extraction time is 30-60min, and the filtration adopts 0.22μm or 0.45μm filter membrane.

10. The method for simultaneously determining the contents of multiple components in Mongolian medicine Xiaozhong Jiuwei Powder according to claim 5, characterized in that: The chromatographic column used was filled with octadecylsilane bonded silica gel, and the column parameters were 250 mm × 4.6 mm, 5 μm.

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