Technical method for realizing anti-counterfeiting traceability by adding artificially synthesized DNA (Deoxyribose Nucleic Acid) probe

Abstract

The invention relates to the technical field of biological information, in particular to a technical method for realizing anti-counterfeiting traceability by adding an artificially synthesized DNA (Deoxyribose Nucleic Acid) probe. The method comprises the following steps: S1, synthesizing a DNA probe through a DNA probe design and synthesis technology; s2, randomly selecting a certain number of DNA probes from the DNA probe password combination as DNA probe passwords; s3, adding the DNA probe password randomly selected each time into a product produced on the same day according to a certain proportion; s4, decoding the DNA probe password in the product through DNA probe detection equipment; and S5, uploading DNA probe decoding data obtained by product detection to an anti-counterfeiting traceability system for data comparison. The length, sequence composition and other characteristics of the DNA probe can be accurately controlled through the DNA probe design and synthesis technology, so that specific application requirements are met. The DNA probe is randomly selected as a password, so that the cracking difficulty can be increased, and the safety is improved.

Description

Technical Field

[0001] The present invention relates to the field of bioinformatics, and in particular to a technical method for realizing anti-counterfeiting and tracing by adding artificially synthesized DNA probes. Background Art

[0002] A technical method to achieve anti-counterfeiting traceability by adding synthetic DNA probes is to add synthetic DNA probes. This technology is based on the principle of molecular recognition in biology and uses a unique DNA sequence as an identifier. Specifically, during the production process, the manufacturer will add a unique DNA sequence (the so-called DNA tag) that has been designed and registered to the product. This DNA sequence is highly specific and difficult to copy or forge, so it can be used as proof of product identity.

[0003] In order to ensure that this DNA probe does not affect the quality of food, beverages or alcohol, these DNA tags are usually specially treated to ensure their stability and safety under different environmental conditions. In addition, their addition amount will be controlled at an extremely low level to avoid any potential risks to human health. When it is necessary to verify the authenticity of the product, the DNA tag can be extracted from the sample by professional testing equipment and compared with the registration information in the database. This process usually includes steps such as sample preparation, DNA extraction, PCR amplification, and sequencing analysis. If a matching DNA sequence is found, the source and authenticity of the product can be confirmed, thereby achieving the purpose of anti-counterfeiting and traceability. This method can not only effectively combat counterfeit and shoddy products, but also help consumers understand the production background of the product, enhance market transparency, and promote food safety management.

[0004] In an existing technical method for achieving anti-counterfeiting and traceability by adding artificially synthesized DNA probes, traditional anti-counterfeiting labels, probes or QR codes are easy to be counterfeited, while methods based on physical or chemical characteristics are often easily bypassed or cracked. Therefore, a technical method for achieving anti-counterfeiting and traceability by adding artificially synthesized DNA probes is provided. Summary of the invention

[0005] The purpose of the present invention is to provide a technical method for anti-counterfeiting and traceability by adding artificially synthesized DNA probes, so as to solve the problem raised in the above background technology that in the existing technical methods for anti-counterfeiting and traceability by adding artificially synthesized DNA probes, traditional anti-counterfeiting labels, probes or QR codes are easy to be counterfeited, while methods based on physical or chemical characteristics are often easy to be bypassed or cracked.

[0006] To achieve the above object, the present invention provides a technical method for anti-counterfeiting and tracing by adding artificially synthesized DNA probes, comprising the following steps:

[0007] S1. Synthesize DNA probes through DNA probe design and synthesis technology;

[0008] S2, randomly selecting a certain number of DNA probes from the DNA probe code combination as DNA probe codes;

[0009] S3, adding the randomly selected DNA probe code each time to the products produced on that day according to a certain ratio;

[0010] S4. Decoding the DNA probe code in the product through DNA probe detection equipment;

[0011] S5. Upload the DNA probe decoding data obtained from product testing to the anti-counterfeiting traceability system for data comparison.

[0012] As a further improvement of the technical solution, in S1, the specific steps of synthesizing the DNA probe by DNA probe synthesis technology are:

[0013] S2.1. Design DNA probe sequence;

[0014] S2.2. Use automated synthesis equipment to synthesize the required DNA sequence.

[0015] S2.3, purifying the synthesized DNA probe;

[0016] S2.4. Use gel electrophoresis or high performance liquid chromatography to detect the purity and concentration of the DNA probe.

[0017] As a further improvement of the technical solution, in S2.4, the specific expression for detecting the purity and concentration of the DNA probe by gel electrophoresis or high performance liquid chromatography is:

[0018] Purity calculation:

[0019]

[0020] Where P represents the purity of the DNA probe; A 260 Indicates the absorbance value at 260nm; A 280 Indicates the absorbance value at 280nm;

[0021] Concentration calculation:

[0022]

[0023] Wherein, C represents the concentration of DNA probe; D represents the dilution factor; and L represents the molar extinction coefficient.

[0024] As a further improvement of the technical solution, in S2, the specific steps of randomly selecting a certain number of DNA probes from the DNA probe password combination as DNA probe passwords are:

[0025] S3.1. First, the person responsible for preserving the DNA probes randomly sorts all the DNA probes in the DNA probe combination in a shuffled order, and the corresponding relationship table between the initial DNA probe serial numbers and the random serial numbers is sealed and kept confidential;

[0026] S3.2, and then another technician randomly selects a certain number of DNA probes from the scrambled DNA probes as DNA probe codes, and the sequence number list of the selected DNA probes is sealed and kept confidential;

[0027] S3.3. Finally, two technicians upload the correspondence table of the initial DNA probe serial numbers and random serial numbers and the serial number list of the selected DNA probes to the anti-counterfeiting traceability system respectively.

[0028] As a further improvement of the technical solution, in S3, the specific steps of adding the randomly selected DNA probe code each time to the products produced on the day according to a certain ratio are:

[0029] S4.1. Calculate the amount of DNA probes required and dissolve the DNA probes in a solvent;

[0030] S4.2, adding the DNA probe solution to the product according to a predetermined ratio;

[0031] S4.3. Mix well to ensure that the DNA probe is evenly distributed.

[0032] As a further improvement of the technical solution, in S4.1, the number of required DNA probes is calculated, and the specific expression for dissolving the DNA probes in the solvent is:

[0033]

[0034] Among them, V p represents the total amount of product; Q represents the total amount of DNA probes to be added; q represents the amount of DNA probes to be added per unit volume of product;

[0035] When the total volume of the prepared DNA probe solution is V s , then the concentration of the solution

[0036] As a further improvement of the technical solution, in S4, the specific steps of decoding the DNA probe code in the product by the DNA probe detection device are:

[0037] S5.1. Collect samples from the product and extract DNA;

[0038] S5.2, loading the extracted DNA sample into the detection device;

[0039] S5.3, reading the DNA probe test results on the testing device;

[0040] S5.4. Analyze the test results and decode the DNA probe.

[0041] As a further improvement of the technical solution, in S5.3, the specific expression of DNA probe detection is:

[0042] I = k·C + b;

[0043] Where, I represents the intensity of the detected fluorescence signal; k represents the proportional constant; b represents the background signal intensity;

[0044] A threshold T is set. When I>T, it is considered that there is a DNA probe and sequence information at the position.

[0045] As a further improvement of the technical solution, in S5.3, the specific expression of DNA probe detection is:

[0046] I = k·C + b;

[0047] Where, I represents the intensity of the detected fluorescence signal; k represents the proportional constant; b represents the background signal intensity;

[0048] A threshold T is set. When I>T, it is considered that there is a DNA probe and sequence information at the position.

[0049] As a further improvement of the technical solution, in S5, the specific steps of uploading the DNA probe decoding data obtained from product testing to the anti-counterfeiting traceability system for data comparison are:

[0050] S6.1. Collect and organize test results;

[0051] S6.2. Upload data to the anti-counterfeiting traceability system;

[0052] S6.3, compare with the standard database in the system;

[0053] S6.4. Evaluate the comparison results and confirm the authenticity of the product.

[0054] Compared with the prior art, the present invention has the following beneficial effects:

[0055] 1. In this technical method of realizing anti-counterfeiting traceability by adding artificially synthesized DNA probes, the length, sequence composition and other characteristics of the DNA probes can be precisely controlled through synthesis technology to meet specific application requirements. Randomly selecting DNA probes as passwords can increase the difficulty of cracking and improve security.

[0056] 2. In this technical method of anti-counterfeiting and tracing by adding artificially synthesized DNA probes, by adding a specific proportion of DNA probe codes to the product, the source of the product can be traced. DNA probe detection equipment can provide high-precision test results and reduce the possibility of false positives or false negatives. The authenticity of the product can be confirmed by comparison to prevent counterfeit and shoddy products from entering the market. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] Figure 1 The figure is a flow chart of the overall method of the present invention. DETAILED DESCRIPTION

[0058] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0059] Example:

[0060] See also Figure 1 As shown, this embodiment provides a technical method for achieving anti-counterfeiting and traceability by adding artificially synthesized DNA probes, comprising the following steps:

[0061] S1. synthesizing a DNA probe by a DNA probe synthesis technique;

[0062] In this example, the specific steps of synthesizing the DNA probe by DNA probe synthesis technology are:

[0063] S2.1. Design DNA probe sequence;

[0064] Specifically, DNA probe design, synthesis and reading specifically include:

[0065] DNA probe design:

[0066] (1) Select the reference area:

[0067] Determine the genome type and reference region for probe design, which are usually highly conserved and easily amplified regions of animal and plant genomes.

[0068] (2) Probe design parameters:

[0069] The Tm value of the probe should be between 55-65°C to ensure a suitable annealing temperature;

[0070] The GC content of the probe should be between 40-60% to avoid the formation of secondary structures;

[0071] The probe length is usually between 18 and 27 bases to ensure specificity and amplification efficiency;

[0072] Make sure that the probe does not form an internal palindrome or be self-complementary.

[0073] (3) Use Primer3 online tool to assist in design:

[0074] Visit the Primer3 website;

[0075] Input reference DNA sequence;

[0076] Set probe design parameters (such as Tm value, GC content, probe length, etc.);

[0077] Run the design program to obtain the probe sequence.

[0078] (4) Verify probe specificity:

[0079] Visit the NCBI BLAST website;

[0080] Select a suitable database (such as nt, nr, etc.);

[0081] Input the designed probe sequence;

[0082] Run a BLAST search to check whether the probe cross-reacts with other non-reference sequences;

[0083] Analyze BLAST results to ensure probe specificity.

[0084] DNA probe synthesis:

[0085] (1) Provide probe sequences to synthesis companies:

[0086] Choose a reliable synthesis company (such as Integrated DNA Technologies, Thermo Fisher Scientific, etc.);

[0087] Log on to the company's website or contact customer service;

[0088] Provide the designed probe sequence and other relevant information (such as purity requirements, quantity, etc.).

[0089] (2) Supervise the synthesis process and ensure quality control:

[0090] Ensure that the company confirms the order and provides an estimated delivery time;

[0091] Require the company to provide synthesis reports, including HPLC purification reports, mass spectrometry analysis reports, etc.;

[0092] Follow up the order status regularly to ensure the synthesis process goes smoothly.

[0093] (3) Receiving and purifying the synthesized probe:

[0094] After receiving the synthesized probe, check whether the packaging is intact and verify the probe information;

[0095] Dissolve the probe using sterile water or TE buffer according to the instructions provided by the company;

[0096] Use high performance liquid chromatography (HPLC) to remove impurities and unreacted starting materials;

[0097] The probe was purified using polyacrylamide gel electrophoresis (PAGE);

[0098] Determine the probe concentration using a UV spectrophotometer (e.g., NanoDrop);

[0099] The purified probes were stored in a -20°C freezer to avoid repeated freezing and thawing.

[0100] Reading of DNA probe:

[0101] DNA probes are detected using various gene detection equipment such as gene chip scanners, gene sequencers and fluorescent quantitative PCR.

[0102] Among them, the first generation Sanger sequencing method includes the following steps:

[0103] PCR amplification: PCR amplification of the target DNA region using the designed probe;

[0104] Purify PCR products: Use a purification kit to remove excess probes and dNTPs;

[0105] Sequencing reaction: Mix the purified PCR product with the sequencing probe, add fluorescently labeled ddNTPs and sequencing enzyme, and perform sequencing reaction;

[0106] Electrophoretic separation: The sequencing products are subjected to capillary electrophoresis and the sequence information is read through the fluorescent signal;

[0107] Data analysis: The sequencing software FinchTV was used to analyze the sequencing results, correct the sequences and align them with the reference sequences.

[0108] The next generation high-throughput sequencing method includes the following steps:

[0109] Library preparation: PCR-amplified DNA fragments are connected to adapters to form a sequencing library;

[0110] Quality control: Check library concentration using Qubit or NanoDrop instrument, and check library size distribution by agarose gel electrophoresis or Bioanalyzer;

[0111] Sequencing: Load the library onto the Illumina MiSeq sequencer for massively parallel sequencing;

[0112] Data analysis: The bioinformatics tool Trimmomatic was used for data processing and sequence alignment.

[0113] Next-generation sequencing includes the following steps:

[0114] Library preparation: Similar to next-generation sequencing, but usually does not require PCR amplification and can directly use long-fragment DNA;

[0115] Sequencing: Use single-molecule real-time sequencing technology PacBio Sequel II / IIe for long-read sequencing;

[0116] Data analysis: SMRT Analysis was used for data processing and assembly to generate high-quality long-read sequences.

[0117] The following is an example of 10 DNA probes, each containing 11 deoxyribonucleotides (bases), as shown in the following table:

[0118] Barcode number DNA barcoding 1 ATCGATCGATC 2 TAGCTAGCTAG 3 GCTAGCTAGCT 4 CGATCGATCGA 5 TATCTATCTAT 6 ATGCTATGCTA 7 GATCGATCGAT 8 CTAGCTAGCTA 9 TAGCATGCTAG 10 CGATGCATGCA

[0119] Specifically, uniquely designed DNA probe sequences can ensure that each product has a unique identifier, thereby improving anti-counterfeiting performance.

[0120] S2.2. Use automated synthesis equipment to synthesize the required DNA sequence.

[0121] Specifically, automated synthesis equipment is mainly based on solid phase synthesis, which uses chemical reactions on a solid phase carrier to gradually construct the required DNA.

[0122] The specific steps include:

[0123] Activation: Activating the active groups on the solid support by chemical reagents;

[0124] Coupling: adding the next nucleotide unit to the end of the sequence;

[0125] End-capping: prevent non-specific reactions;

[0126] Cleaning: Removal of excess chemicals and by-products;

[0127] Repeat: Repeat the above steps until the complete sequence is synthesized.

[0128] S2.3, purifying the synthesized DNA probe;

[0129] Specifically, purification refers to a series of steps taken after the synthesis of the DNA probe to remove by-products, unreacted chemical reagents and other impurities to ensure the purity and quality of the DNA probe.

[0130] S2.4. Use gel electrophoresis or high performance liquid chromatography to detect the purity and concentration of the DNA probe.

[0131] In this example, the specific expression for detecting the purity and concentration of the DNA probe by gel electrophoresis or high performance liquid chromatography is:

[0132] Purity calculation:

[0133]

[0134] Where P represents the purity of the DNA probe; A 260 Indicates the absorbance value at 260nm; A 280 Indicates the absorbance value at 280nm;

[0135] Concentration calculation:

[0136]

[0137] Wherein, C represents the concentration of DNA probe; D represents the dilution factor; and L represents the molar extinction coefficient.

[0138] Each specific DNA probe sequence should be unique, and aggregation should be avoided when mixed in liquid. Each DNA probe is stored separately, and all DNA probes synthesized in the same batch together become a DNA probe password combination. The number of DNA probes in a DNA probe password combination can be flexibly determined according to actual conditions, generally should be more than 100, and should be regularly updated as a whole.

[0139] S2, randomly selecting a certain number of DNA probes from the DNA probe code combination as DNA probe codes;

[0140] In this example, the specific steps of randomly selecting a certain number of DNA probes from the DNA probe code combination as DNA probe codes are:

[0141] S3.1. First, the person responsible for preserving the DNA probes randomly sorts all the DNA probes in the DNA probe combination in a shuffled order, and the corresponding relationship table between the initial DNA probe serial numbers and the random serial numbers is sealed and kept confidential;

[0142] S3.2, and then another technician randomly selects a certain number of DNA probes from the scrambled DNA probes as DNA probe codes, and the sequence number list of the selected DNA probes is sealed and kept confidential;

[0143] S3.3. Finally, two technicians upload the correspondence table of the initial DNA probe serial numbers and random serial numbers and the serial number list of the selected DNA probes to the anti-counterfeiting traceability system respectively.

[0144] S3, adding the randomly selected DNA probe code each time to the products produced on that day according to a certain ratio;

[0145] In this example, the specific steps of adding the randomly selected DNA probe code to the products produced on the same day according to a certain ratio are as follows:

[0146] S4.1. Calculate the amount of DNA probes required and dissolve the DNA probes in a solvent;

[0147] In this example, the amount of DNA probe required is calculated and the DNA probe is dissolved in a solvent. The specific expression is:

[0148]

[0149] Among them, V p represents the total amount of product; Q represents the total amount of DNA probes to be added; q represents the amount of DNA probes to be added per unit volume of product;

[0150] When the total volume of the prepared DNA probe solution is V s , then the concentration of the solution

[0151] Specifically, by accurately calculating the number of required DNA probes, it is possible to ensure that the amount of DNA probes added each time is consistent, thereby improving the consistency and reliability of the anti-counterfeiting label.

[0152] S4.2, adding the DNA probe solution to the product according to a predetermined ratio;

[0153] S4.3. Mix well to ensure that the DNA probe is evenly distributed.

[0154] Specifically, by fully mixing, it can be ensured that the DNA probes are evenly distributed throughout the product without local aggregation. The evenly distributed DNA probes enable any part of the sample to represent the anti-counterfeiting information of the entire product during subsequent testing, thereby improving the reliability and accuracy of the test. It prevents the DNA probes from being overly concentrated in certain areas, thereby causing excessive local concentrations and affecting the test results.

[0155] S4. Decoding the DNA probe code in the product through DNA probe detection equipment;

[0156] Among them, the equipment for DNA probe detection includes gene chip scanners, gene sequencers and fluorescent quantitative PCR.

[0157] In this example, the specific steps of decoding the DNA probe code in the product by the DNA probe detection device are:

[0158] S5.1. Collect samples from the product and extract DNA;

[0159] S5.2. Read the signal strength on the detection device and record the data;

[0160] Specifically, the DNA probe detection equipment has high sensitivity and can detect trace amounts of DNA probe signals, ensuring that even low concentrations of DNA probes in samples can be detected. The DNA probe detection equipment can process multiple samples at the same time, improving detection efficiency and is suitable for rapid screening of large quantities of samples. Accurate reading of signal strength helps improve the accuracy of test results and reduce misjudgments.

[0161] S5.3, performing DNA probe detection on a DNA probe detection device;

[0162] In this example, the specific expression of DNA probe detection is:

[0163] I = k·C + b;

[0164] Where, I represents the intensity of the detected fluorescence signal; k represents the proportional constant; b represents the background signal intensity;

[0165] A threshold T is set. When I>T, it is considered that there is a DNA probe and sequence information at the position.

[0166] S5.4. Analyze the test results and decode the DNA probe.

[0167] In this example, the specific expression for analyzing the test results and decoding the DNA probe is:

[0168]

[0169]

[0170] Among them, Score represents the similarity between two sequences; n represents the total number of matched base pairs or amino acid residues; i represents the index variable; s i represents the match and mismatch scores; g represents the gap penalty; Similarity represents the sequence similarity percentage; MatchCount represents the number of matched bases; QueryLength represents the length of the query sequence.

[0171] S5. Upload the DNA probe decoding data obtained from product testing to the anti-counterfeiting traceability system for data comparison.

[0172] In this example, the specific steps for uploading the DNA probe decoding data obtained from product testing to the anti-counterfeiting traceability system for data comparison are as follows:

[0173] S6.1. Collect and organize test results;

[0174] S6.2. Upload data to the anti-counterfeiting traceability system;

[0175] Specifically, the anti-counterfeiting traceability system is a technical solution for verifying the authenticity of products and tracking their entire production and circulation process. It uses a series of technologies and methods to ensure that every link of the product from raw material procurement, production and processing, packaging, warehousing and logistics to sales terminals can be recorded, tracked and verified.

[0176] S6.3, compare with the standard database in the system;

[0177] Specifically, the comparison is performed through a string matching scoring method;

[0178]

[0179] Among them, S(x,y) represents the matching score of strings x and y; m represents the sequence length; s(x j ,y j ) represents the single nucleotide match score at position j.

[0180] Comparison with the standard database can accurately determine the authenticity of the DNA probe in the sample and improve the reliability of the test results. By comparing the DNA probe sequence in the sample, it can reduce the human error that may be caused by manual comparison and improve the accuracy.

[0181] S6.4. Evaluate the comparison results and confirm the authenticity of the product.

[0182] Specifically, by evaluating and comparing the results, a clear conclusion on the authenticity of the product can be drawn, thus improving the anti-counterfeiting effect. The evaluation results provide a scientific basis for decision-making, helping companies or regulatory agencies make correct judgments. The evaluation results can be fed back to the production process to help improve the production process or strengthen quality control.

[0183] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only preferred examples of the present invention, and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and the specific length, sequence, quantity, design method and synthesis method of the DNA probe may be adjusted according to specific circumstances, and the detection equipment and detection method of the DNA probe may be selected and improved as needed, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A technical method for achieving anti-counterfeiting and traceability by adding artificially synthesized DNA probes, characterized in that: The following steps are involved: S1. Synthesize DNA probes through DNA probe design and synthesis technology; S2, randomly selecting a certain number of DNA probes from the DNA probe code combination as DNA probe codes; S3, adding the randomly selected DNA probe code each time to the products produced on that day according to a certain ratio; S4. Decoding the DNA probe code in the product through DNA probe detection equipment; S5. Upload the DNA probe decoding data obtained from product testing to the anti-counterfeiting traceability system for data comparison.

2. The technical method for realizing anti-counterfeiting and tracing by adding artificially synthesized DNA probes according to claim 1, characterized in that: In S1, the specific steps of synthesizing the DNA probe by DNA probe synthesis technology are: S2.

1. Design DNA probe sequence; S2.

2. Use automated synthesis equipment to synthesize the required DNA sequence. S2.3, purifying the synthesized DNA probe; S2.

4. Use gel electrophoresis or high performance liquid chromatography to detect the purity and concentration of the DNA probe.

3. The technical method for realizing anti-counterfeiting and tracing by adding artificially synthesized DNA probes according to claim 2, characterized in that: In S2.4, the specific expression for detecting the purity and concentration of the DNA probe by gel electrophoresis or high performance liquid chromatography is: Purity calculation: Where P represents the purity of the DNA probe; A 260 Indicates the absorbance value at 260nm; A 280 Indicates the absorbance value at 280nm; Concentration calculation: Wherein, C represents the concentration of DNA probe; D represents the dilution factor; and L represents the molar extinction coefficient.

4. The technical method for realizing anti-counterfeiting and tracing by adding artificially synthesized DNA probes according to claim 1, characterized in that: In S2, the specific steps of randomly selecting a certain number of DNA probes from the DNA probe code combination as DNA probe codes are: S3.

1. First, the person responsible for preserving the DNA probes randomly sorts all the DNA probes in the DNA probe combination in a shuffled order, and the corresponding relationship table between the initial DNA probe serial numbers and the random serial numbers is sealed and kept confidential; S3.2, and then another technician randomly selects a certain number of DNA probes from the scrambled DNA probes as DNA probe codes, and the sequence number list of the selected DNA probes is sealed and kept confidential; S3.

3. Finally, two technicians upload the correspondence table of the initial DNA probe serial numbers and random serial numbers and the serial number list of the selected DNA probes to the anti-counterfeiting traceability system respectively.

5. The technical method for realizing anti-counterfeiting and tracing by adding artificially synthesized DNA probes according to claim 1, characterized in that: In S3, the specific steps of adding the randomly selected DNA probe codes to the products produced on the day in a certain proportion are: S4.

1. Calculate the amount of DNA probes required and dissolve the DNA probes in a solvent; S4.2, adding the DNA probe solution to the product according to a predetermined ratio; S4.

3. Mix well to ensure that the DNA probe is evenly distributed.

6. The technical method for realizing anti-counterfeiting and tracing by adding artificially synthesized DNA probes according to claim 5, characterized in that: In S4.1, the number of required DNA probes is calculated, and the DNA probes are dissolved in a solvent. The specific expression is: Among them, V p represents the total amount of product; Q represents the total amount of DNA probes to be added; q represents the amount of DNA probes to be added per unit volume of product; When the total volume of the prepared DNA probe solution is V s , then the concentration of the solution 7. The technical method for realizing anti-counterfeiting and tracing by adding artificially synthesized DNA probes according to claim 1, characterized in that: In S4, the specific steps of decoding the DNA probe code in the product by the DNA probe detection device are: S5.

1. Collect samples from the product and extract DNA; S5.2, read the signal intensity on the microfluidic chip and record the data; S5.3, DNA probe detection on microfluidic chip; S5.

4. Analyze the test results and decode the DNA probe.

8. The technical method for realizing anti-counterfeiting and tracing by adding artificially synthesized DNA probes according to claim 7, characterized in that: In S5.3, the specific expression of DNA probe detection is: I = k·C + b; Where, I represents the intensity of the detected fluorescence signal; k represents the proportional constant; b represents the background signal intensity; A threshold T is set. When I>T, it is considered that there is a DNA probe and sequence information at that position.

9. The technical method for realizing anti-counterfeiting and tracing by adding artificially synthesized DNA probes according to claim 7, characterized in that: In S5.4, the specific expression for analyzing the test results and decoding the DNA probe is: Among them, Score represents the similarity between two sequences; n represents the total number of matched base pairs or amino acid residues; i represents the index variable; s i represents the match and mismatch scores; g represents the gap penalty; Similarity represents the sequence similarity percentage; matchCount represents the number of matched bases; QueryLength represents the length of the query sequence.

10. The technical method for realizing anti-counterfeiting and tracing by adding artificially synthesized DNA probes according to claim 1, characterized in that: In S5, the specific steps of uploading the DNA probe decoding data obtained from product testing to the anti-counterfeiting traceability system for data comparison are: S6.

1. Collect and organize test results; S6.

2. Upload data to the anti-counterfeiting traceability system; S6.3, compare with the standard database in the system; S6.

4. Evaluate the comparison results and confirm the authenticity of the product.

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