Multifunctional oral cavity desensitization gel and preparation method thereof

Abstract

The preparation method comprises the following steps: adding an anti-allergy agent into purified water in proportion, stirring and dissolving, adjusting the pH value of the solution by using a citric acid solution, and adding a bioactive mineral substance into the solution to prepare a component I; weighing a plurality of materials and a cross-linking agent, and adding purified water to dissolve the materials and the cross-linking agent to prepare a component II; mixing the component I and the component II, and cross-linking the bioactive mineral substances and the peptides to prepare an active microcarrier; weighing glycerol, sodium carboxymethyl cellulose, xanthan gum and guar gum, uniformly stirring, and slowly adding into the active microcarrier system to prepare a gel system; and adding other components into the gel system prepared in the previous step. Through an active microcarrier preparation process, on one hand, antibacterial and anti-allergy components are mixed into a porous structure of a bioactive mineral substance, so that the purposes of slowly releasing and prolonging the action time are achieved; on the other hand, the bioactive minerals and the oligopeptide are crosslinked, and the oligopeptide induces tooth remineralization, so that the desensitization purpose is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of oral products, and in particular to a multifunctional oral desensitization gel and a preparation method thereof. Background Art

[0002] Dentin sensitivity refers to the short and sharp pain of exposed dentin in response to external stimuli, including temperature stimulation, air blowing stimulation, mechanical stimulation or chemical stimulation. There are many reasons for dentin sensitivity, including tooth erosion, gum recession, caries, enamel damage and dentin exposure caused by other reasons. With the improvement of living standards, changes in dietary structure and the increase of age, the frequency of tooth sensitivity is more prominent. According to statistics, 29.7% of adults in my country have different degrees of dentin sensitivity, coupled with common oral inflammations such as periodontitis and oral ulcers, which seriously affect people's quality of life.

[0003] In addition to medication and surgical treatment, the commonly used method to relieve dentin sensitivity is to use anti-sensitive toothpaste or tooth desensitizer. The desensitization principle is mainly two aspects: one is to block the dentinal tubules and reduce the fluidity of the fluid in the tubules to relieve the sensitivity of the dentin and pulp nerve endings; the other is to calm the pulp nerves and use potassium salts and other substances to reduce the excitability of the pulp nerve endings to reduce pain and play a role in reducing dentin sensitivity. However, there are shortcomings in the products available on the market. The desensitization effect takes a long time to take effect, lasts for a short time, and the effect is short-lived and not significant.

[0004] In addition, the human oral cavity is a relatively complex internal environment, containing harmful bacteria and beneficial bacteria. If it is not properly cared for, it is easy to disrupt the balance of the oral microenvironment. In particular, acid-producing anaerobic bacteria will cause the oral pH to decrease, thereby causing the growth of harmful bacteria. When a large number of harmful bacteria dominate, the periodontal tissue will be severely damaged, thereby aggravating tooth sensitivity, leading to oral ulcers, bleeding gums, gingivitis and many other problems, affecting the patient's eating and daily life.

[0005] In summary, it is particularly important to develop a multifunctional oral desensitization gel with high safety, significant effects, anti-allergic and anti-inflammatory properties, and a short desensitization effect with a long duration and long-lasting effect. Summary of the invention

[0006] The purpose of the present invention is to provide a multifunctional oral desensitization gel and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0007] The above technical objectives of the present invention are achieved through the following technical solutions:

[0008] A method for preparing a multifunctional oral desensitization gel, the preparation method is as follows:

[0009] Step 1: add the anti-allergic agent to 15%-20% purified water in proportion, stir and dissolve, adjust the solution pH to between 7 and 10 with 0.5-1M citric acid solution, add 3%-8% by mass of bioactive minerals to the above solution, treat with a 19-25kHZ ultrasonic disperser for 2-10 minutes, mix evenly, so that the anti-allergic agent can fully enter the porous structure of the bioactive minerals, and prepare component I;

[0010] Step 2: Weigh 0.5-1% collagen peptide, 0.2-1% casein phosphopeptide, 0.1-1% antimicrobial peptide and cross-linking agent by mass, add 30-40% purified water, stir and dissolve, stir and disperse for 5-20 minutes, cross-link, and prepare component II;

[0011] Step 3: Mix component I and component II, stir at 50°C-60°C, 100-200 rpm for 10-30 min. Cross-link the bioactive minerals and peptides to obtain active microcarriers;

[0012] Step 4: weigh 8-20% by mass of glycerol, 0.2-0.8% by mass of sodium carboxymethyl cellulose, 0.1-0.5% by mass of xanthan gum, and 0.1-0.5% by mass of guar gum, stir them evenly, and then slowly add them into the system of step 3, keep stirring at 50°C-60°C until the system is uniform, and the total heating time shall not exceed 2 hours to obtain a gel system;

[0013] Step 5: Add other components to the gel system prepared in step 4: including 2-5% by mass of an abrasive, 0.1%-0.4% of a preservative, 0.01%-0.05% of an antioxidant, 0.2%-1.5% of an anti-caries agent and the remainder being purified water, to finally obtain a multifunctional oral desensitizing gel with a dynamic viscosity greater than 23000 mPa·s and a dental tubule blocking rate greater than 70%.

[0014] Preferably, in step 1, the anti-allergic agent comprises 0.5%-1.5% of L-arginine and 0.1%-0.5% of dipotassium glycyrrhizinate.

[0015] Preferably, in step 1, the bioactive minerals include nano-hydroxyapatite, β-tricalcium phosphate, and nano-silicon dioxide, the mass ratio of the three is 2:1:(0.1-0.5), the particle size of nano-hydroxyapatite is 200-500 nanometers; the particle size of nano-silicon dioxide is 50-100 nanometers, and the hydroxyapatite can be spherical or short rod-shaped.

[0016] Preferably, in step 2, the molecular weight of collagen peptide is 10KDa-50KDa, the molecular weight of casein phosphopeptide is 2KDa-3KDa, and the molecular weight of antimicrobial peptide is 2KDa-7KDa. The cross-linking agent is 3-6% potassium citrate.

[0017] Preferably, in step 5, the abrasive is silicon dioxide with a diameter of 10-20 microns; the preservative is one or more of sodium ethylparaben, sodium butylparaben, and ε-polylysine hydrochloride; the antioxidant is one or more of vitamin E, sodium phytate, and disodium ethylenediaminetetraacetic acid; and the anti-caries agent is one or more of erythritol, xylitol, and sorbitol liquid.

[0018] Preferably, a multifunctional oral desensitizing gel is obtained by the preparation method described above.

[0019] Beneficial effects:

[0020] 1. The present invention prepares a multifunctional oral desensitizing gel that has the functions of preventing bacteria, resisting bacteria, resisting inflammation, desensitizing, and promoting dentin repair;

[0021] 2. Creatively prepare an active microcarrier, mix antibacterial agents and anti-allergic agents into the porous structure of bioactive minerals, and make it by cross-linking with short peptides. On the one hand, it guides the active ingredients directly to the site of action, realizes the sustained release of each ingredient, and reduces cytotoxicity; on the other hand, it simulates the natural mineralization of dentin, provides an environment of calcium and phosphate, induces dentin adsorption through short peptides, promotes the growth of hydroxyapatite crystals, promotes remineralization, blocks the essence of dentine tubules, and provides potassium elements that can calm the pulp nerves, thereby achieving the effect of reducing tooth sensitivity;

[0022] 3. Multiple types of glue are used for compounding to increase the viscosity of the product, making it easier for the gel to cover the oral surface to prevent bacteria from eroding teeth;

[0023] 4. It provides an alkaline oral environment, avoids excessive growth of acidic bacteria, erodes teeth, strengthens gums, improves oral microenvironment, and enhances the comprehensive anti-allergic ability of the oral cavity;

[0024] 5. This product does not contain fluorine and strontium, is safe and suitable for children. DETAILED DESCRIPTION

[0025] The following is only a preferred embodiment of the present invention, and the protection scope is not limited to this embodiment. All technical solutions under the concept of the present invention should belong to the protection scope of the present invention. At the same time, it should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

[0026] Embodiment 1:

[0027] Step 1: Weigh 1.5 g of L-arginine and 0.1 g of dipotassium glycyrrhizinate, add 15 g of purified water and stir to dissolve. Add 0.5 M citric acid solution to adjust the solution pH to 9, weigh 3 g of hydroxyapatite with a particle size of 500 nanometers, 1.5 g of β-tricalcium phosphate, and 0.2 g of silica with a particle size of 50 nanometers, add the above solution, and treat with a 25 kHz ultrasonic disperser for 5 minutes to prepare component I.

[0028] Step 2: Weigh 1 g of collagen peptide, 1 g of casein phosphopeptide, 0.2 g of antimicrobial peptide and 5 g of potassium citrate, add 40 g of purified water and stir to dissolve, stir and disperse at 2800 rpm in a disperser for 10 min to prepare component II.

[0029] Step 3: Mix component I and component II, stir at a low speed of 150 rpm at 50°C for 10 min to prepare active microcarriers.

[0030] Step 4: Weigh 15 g of glycerol, 0.2 g of sodium carboxymethyl cellulose, 0.3 g of xanthan gum, and 0.25 g of guar gum, stir evenly, and then slowly add them to the system in step 2. During the process, keep stirring at 50°C to 60°C until the system is uniform, to prepare a gel system.

[0031] Step 5: Add 4 g of micronized silicon dioxide, 0.05 g of sodium ethylparaben, 0.05 g of sodium butylparaben, 0.002 g of ε-polylysine hydrochloride, 0.02 g of sodium phytate, 0.01 g of vitamin E, 0.2 g of erythritol and 9.02 g of purified water to the gel system prepared in step 4 to finally obtain a multifunctional oral desensitization gel.

[0032] Embodiment 2:

[0033] Step 1: Weigh 1.5 g of L-arginine and 0.2 g of dipotassium glycyrrhizinate, add 20 g of purified water and stir to dissolve. Add 0.6 M citric acid solution to adjust the solution pH to 9, weigh 2 g of hydroxyapatite with a particle size of 200 nanometers, 1 g of β-tricalcium phosphate, and 0.2 g of silicon dioxide with a particle size of 100 nanometers, add the above solution, and treat with a 25 kHz ultrasonic disperser for 5 minutes to prepare component I.

[0034] Step 2: Weigh 1 g of collagen peptide, 0.5 g of casein phosphopeptide, 0.3 g of antimicrobial peptide and 5 g of potassium citrate, add 40 g of purified water and stir to dissolve, stir and disperse at 2800 rpm in a disperser for 10 min to prepare component II.

[0035] Step 3: Mix component I and component II, stir at a low speed of 150 rpm at 50°C for 10 min to prepare an active carrier.

[0036] Step 4: Weigh 15 g of glycerol, 0.5 g of sodium carboxymethyl cellulose, 0.3 g of xanthan gum, and 0.25 g of guar gum, stir evenly, and then slowly add them to the system in step 2. During the process, keep stirring at 50°C to 60°C until the system is uniform, to prepare a gel system.

[0037] Step 5: Add 4 g of micronized silicon dioxide, 0.05 g of sodium ethylparaben, 0.01 g of ε-polylysine hydrochloride, 0.02 g of sodium phytate, 0.01 g of disodium ethylenediaminetetraacetate, 0.2 g of sorbitol and 6.2 g of purified water to the gel system prepared in step 4 to finally obtain a multifunctional oral desensitization gel.

[0038] Embodiment 3:

[0039] Step 1: Weigh 1.5 g of L-arginine and 0.1 g of dipotassium glycyrrhizinate, add 15 g of purified water and stir to dissolve. Add 1 M citric acid solution to adjust the solution pH to 7, weigh 3 g of hydroxyapatite with a particle size of 500 nanometers, 1.5 g of β-tricalcium phosphate, and 0.2 g of silicon dioxide with a particle size of 50 nanometers, add the above solution, and treat with a 25 kHz ultrasonic disperser for 5 minutes to prepare component I.

[0040] Step 2: Weigh 1 g of collagen peptide, 0.5 g of casein phosphopeptide, 0.3 g of antimicrobial peptide and 6 g of potassium citrate, add 40 g of purified water and stir to dissolve, stir and disperse at 2800 rpm in a disperser for 10 min to prepare component II.

[0041] Step 3: Mix component I and component II, stir at a low speed of 150 rpm at 50°C for 10 min to prepare an active carrier.

[0042] Step 4: Weigh 20 g of glycerol, 0.8 g of sodium carboxymethyl cellulose, 0.3 g of xanthan gum, and 0.1 g of guar gum, stir evenly, and then slowly add them to the system in step 2. During the process, keep stirring at 50°C to 60°C until the system is uniform, to prepare a gel system.

[0043] Step 5: Add 5 g of micronized silicon dioxide, 0.1 g of sodium ethylparaben, 0.05 g of sodium butylparaben, 0.02 g of vitamin E, 0.01 g of disodium ethylenediaminetetraacetate, 1 g of erythritol and 2.5 g of purified water to the gel system prepared in step 4 to finally obtain a multifunctional oral desensitization gel.

[0044] Embodiment 4:

[0045] Step 1: Weigh 1.5 g of L-arginine and 0.1 g of dipotassium glycyrrhizinate, add 15 g of purified water and stir to dissolve. Add 0.5 M citric acid solution to adjust the solution pH to 9, weigh 3 g of hydroxyapatite with a particle size of 500 nanometers, 1.5 g of β-tricalcium phosphate, and 0.2 g of silica with a particle size of 50 nanometers, add the above solution, and treat with a 25 kHz ultrasonic disperser for 5 minutes to prepare component I.

[0046] Step 2: Weigh 1 g of collagen peptide, 1 g of casein phosphopeptide, 0.2 g of antimicrobial peptide and 5 g of potassium citrate, add 40 g of purified water and stir to dissolve, stir and disperse at 2800 rpm in a disperser for 10 min to prepare component II.

[0047] Step 3: Mix component I and component II, stir at a low speed of 150 rpm at 50°C for 10 min to prepare an active carrier.

[0048] Step 4: Weigh 15 g of propylene glycol, 0.2 g of sodium carboxymethyl cellulose, 0.1 g of xanthan gum, and 0.1 g of guar gum, stir evenly, and then slowly add them to the system in step 2. During the process, keep stirring at 50°C to 60°C until the system is uniform, to prepare a gel system.

[0049] Step 5: Add 4 g of micronized silicon dioxide, 0.05 g of sodium butylparaben, 0.01 g of ε-polylysine hydrochloride, 0.02 g of sodium phytate, 0.2 g of xylitol and 9.32 g of purified water to the gel system prepared in step 4 to finally obtain a multifunctional oral desensitization gel.

[0050] Comparative Example 1:

[0051] Step 1: Weigh 1.5 g of L-arginine, add 15 g of purified water and stir to dissolve. Add 0.5 M citric acid solution to adjust the solution pH to 9, weigh 3 g of hydroxyapatite with a particle size of 500 nanometers, 1.5 g of β-tricalcium phosphate, and 0.2 g of silica with a particle size of 50 nanometers, add the above solution, and treat with a 25 kHz ultrasonic disperser for 5 minutes to prepare component I.

[0052] Step 2: Weigh 1 g of collagen peptide and 1 g of casein phosphopeptide, add 40 g of purified water and stir to dissolve, stir and disperse at 2800 rpm in a disperser for 10 min to prepare component II.

[0053] Step 3: Mix component I and component II, stir at a low speed of 150 rpm at 50°C for 10 min to prepare an active carrier.

[0054] Step 4: Weigh 15 g of glycerol, 0.2 g of sodium carboxymethyl cellulose, 0.3 g of xanthan gum, and 0.25 g of guar gum, stir evenly, and then slowly add them to the system in step 2. During the process, keep stirring at 50°C to 60°C until the system is uniform, to prepare a gel system.

[0055] Step 5: Add 4 g of micronized silicon dioxide, 0.05 g of sodium ethylparaben, 0.05 g of sodium butylparaben, 0.002 g of ε-polylysine hydrochloride, 0.02 g of sodium phytate, 0.2 g of erythritol and 14.22 g of purified water to the gel system prepared in step 4 to finally obtain a multifunctional oral desensitization gel.

[0056] Comparative Example 2:

[0057] Step 1: Weigh 1.5 g of L-arginine and 0.1 g of dipotassium glycyrrhizinate, add 15 g of purified water and stir to dissolve. Add 1 M citric acid solution to adjust the solution pH to 9, weigh 3 g of hydroxyapatite with a particle size of 500 nanometers, 1.5 g of β-tricalcium phosphate, and 0.2 g of silicon dioxide with a particle size of 50 nanometers, add the above solution, and treat with a 25 kHz ultrasonic disperser for 5 minutes to prepare component I.

[0058] Step 2: Weigh 1 g of collagen peptide, 1 g of casein phosphopeptide, 0.2 g of antimicrobial peptide and 5 g of sodium citrate, add 40 g of purified water and stir to dissolve, stir and disperse at 2800 rpm in a disperser for 10 min to prepare component II.

[0059] Step 3: Mix component I and component II, stir at a low speed of 150 rpm at 50°C for 10 min to prepare an active carrier.

[0060] Step 4: Weigh 15 g of propylene glycol, 0.2 g of sodium carboxymethyl cellulose, 0.1 g of xanthan gum, and 0.1 g of guar gum, stir evenly, and then slowly add them to the system in step 2. During the process, keep stirring at 50°C to 60°C until the system is uniform, to prepare a gel system.

[0061] Step 5: Add 4 g of micronized silicon dioxide, 0.05 g of sodium ethylparaben, 0.05 g of sodium butylparaben, 0.002 g of ε-polylysine hydrochloride, 0.02 g of sodium phytate, 0.2 g of erythritol and 9.02 g of purified water to the gel system prepared in step 4 to finally obtain a multifunctional oral desensitization gel.

[0062] Comparative Example 3:

[0063] Step 1: Weigh 1.5 g of L-arginine and 0.1 g of dipotassium glycyrrhizinate, add 15 g of purified water and stir to dissolve. Add 1 M citric acid solution to adjust the solution pH to 5, weigh 3 g of hydroxyapatite with a particle size of 500 nanometers, 1.5 g of β-tricalcium phosphate, and 0.2 g of silicon dioxide with a particle size of 50 nanometers, add the above solution, and treat with a 25 kHz ultrasonic disperser for 5 minutes to prepare component I.

[0064] Step 2: Weigh 1 g of collagen peptide, 1 g of casein phosphopeptide, 0.2 g of antimicrobial peptide and 5 g of potassium citrate, add 40 g of purified water and stir to dissolve, stir and disperse at 2800 rpm in a disperser for 10 min to prepare component II.

[0065] Step 3: Mix component I and component II, stir at a low speed of 150 rpm at 50°C for 10 min to prepare an active carrier.

[0066] Step 4: Weigh 15 g of glycerol, 0.2 g of sodium carboxymethyl cellulose, 0.3 g of xanthan gum, and 0.25 g of guar gum, stir evenly, and then slowly add them to the system in step 2. During the process, keep stirring at 50°C to 60°C until the system is uniform, to prepare a gel system.

[0067] Step 5: Add 4 g of micronized silicon dioxide, 0.05 g of sodium ethylparaben, 0.05 g of sodium butylparaben, 0.002 g of ε-polylysine hydrochloride, 0.02 g of sodium phytate, 0.2 g of erythritol and 6.62 g of purified water to the gel system prepared in step 4 to finally obtain a multifunctional oral desensitization gel.

[0068] Effect embodiment 1:

[0069] The therapeutic effect of the desensitizing gel prepared by the present invention on patients with dentin hypersensitivity was tested.

[0070] Test method:

[0071] During the test period, no other toothpaste, mouthwash, desensitization drugs were used, antibiotics, immune preparations, etc. were not taken, and no treatments that affect tooth sensitivity, such as cleaning, scaling, and vital pulp filling, were performed. 140 patients with dentin hypersensitivity were randomly divided into 7 groups, 20 people in each group, and cold air was blown vertically for 1 second at a distance of 5mm from the buccal surface of the sensitive teeth of the subjects to check the degree of tooth hypersensitivity. 3g of the desensitizing gel prepared in Examples 1-4 and Comparative Examples 1-3 were respectively applied to the sensitive tooth surface of the subjects. After keeping for 3 minutes, the desensitization effect was immediately checked with a probe, and the sensitivity was recorded. Each group of subjects continued to use the corresponding desensitizing gel, once in the morning and once in the evening, and came for a follow-up visit after a total of 2 weeks of treatment. The desensitization effect was checked again with a probe, and the sensitivity was recorded. After the follow-up visit, the patients were advised to stop using the desensitizing gel, and come for a follow-up visit again after 10 days, and the probability of symptom recurrence in each group was recorded.

[0072] Examination criteria for tooth sensitivity

[0073] Mild: Mild sensitivity or sourness to cold stimulation and probes in the allergic area;

[0074] Moderate: There is pain in response to cold stimulation, and pain when the probe is used in the allergic area, but the patient can tolerate the probe examination.

[0075] Severe: Unable to rinse mouth with hot or cold water, extremely sensitive to sweet and sour foods, severe pain in the allergic area of ​​the probe, unbearable.

[0076] Efficacy evaluation criteria:

[0077] Markedly effective: Patients report that their pain and soreness from cold, hot, sour, and sweet stimulations disappear after taking the medicine, and the symptoms completely or basically disappear when the original allergic area is examined with a probe;

[0078] Effective: Patients report that their symptoms of cold, hot, sour, and sweet stimulation and pain are alleviated after taking the medicine compared with before taking the medicine, and the symptoms of the original allergic area are alleviated or improved when the probe is used to check;

[0079] Ineffective: Symptoms do not improve after medication.

[0080] The calculation method of the therapeutic significant efficacy and effective rate involved in the present invention is as follows:

[0081] Significant efficacy (%) = number of significant effects / total number of treatments × 100%;

[0082] Effective rate (%) = (number of markedly effective cases + number of effective cases) / total number of treatments × 100%.

[0083] Test results:

[0084] Table 1 Clinical trial results of desensitizing gel for the treatment of dentin hypersensitivity

[0085]

[0086]

[0087] Analysis of results: It can be seen from the data in Table 1 that the desensitizing gel used in the above Examples 1-4 is more effective than the desensitizing gel in Comparative Examples 1-3, and the subsequent relapse rate is also lower. Example 1 in the present application is the optimal embodiment of the present application, and its immediate treatment effect and the treatment effect after 2 weeks are the best, and its effect is significantly higher than that of the other desensitizing gels; among all the comparative examples, Comparative Example 3 has the worst immediate and continuous treatment effects due to the acidic pH of the system, and the effective ingredients do not play their due role, and the relapse rate is the highest 10 days after stopping the treatment; Comparative Example 1 does not add cross-linking agent and antimicrobial peptide, so that the active microcarrier-induced tooth remineralization and sustained release effect do not meet the expectations, and relapse occurs quickly after stopping the treatment; Comparative Example 2 reduces the introduction of potassium ions, and the desensitization effect is slightly worse than that of the embodiment, but basically maintains most of the efficacy of the desensitizing gel, and the relapse rate is also the lowest among the 3 comparative desensitization gels.

[0088] Effect Example 2: Detection of Desensitization Gel Bacterial Inhibition Rate

[0089] Purpose of the test:

[0090] Streptococcus mutans is a common dental caries pathogen. This experiment indirectly indicates the preventive and therapeutic effects of the desensitizing gel on dental caries by verifying the inhibitory effect of the desensitizing paste on Streptococcus mutans.

[0091] Test method:

[0092] (1) Subculture the mutans Streptococcus to form a mutans Streptococcus colony and dilute the mutans Streptococcus liquid in the logarithmic phase to 1.0×10 6 CFU / mL, add 0.1mL of bacterial solution to the sterilized 24-well plate, place the 24-well plate in an anaerobic incubator at 37℃ and culture for 24 hours without adding fresh culture medium, and record the number of mutans streptococci at this time;

[0093] (2) Take 0.1 g of the desensitizing gel prepared in Examples 1-4 and Comparative Examples 1-3 of the present application, dilute it 10 times with distilled water, and drop it into the 24-well plates that have completed the culture for antibacterial test. After culturing for 24 hours under the same conditions, record the number of Streptococcus mutans.

[0094] Test results:

[0095] The percentage reduction of Streptococcus mutans bacteria before and after desensitization gel culture is shown in the following table:

[0096] Table 2 The inhibitory effect of desensitization gel on Streptococcus mutans

[0097] Experimental Group Bacterial inhibition percentage Example 1 42％ Example 2 48％ Example 3 46％ Example 4 40％ Comparative Example 1 27％ Comparative Example 2 38％ Comparative Example 3 15％

[0098] Analysis of results: From the data in Table 2, it can be concluded that when tested under the same environment, Examples 1-4 are better at inhibiting bacteria than Comparative Examples 1-3, and the desensitizing gel of Example 2 has the best bacterial inhibition effect; the desensitizing gel of Comparative Example 3 has an acidic pH, so that the anti-allergic and antibacterial components do not play their due role, and the bacterial inhibition effect is the worst; Comparative Example 1 does not add cross-linking agents and antimicrobial peptides, so that the active microcarriers do not fully play their role, and the antibacterial effect is not good; Comparative Example 2 reduces the introduction of potassium ions, and the antibacterial effect is slightly worse than that of the embodiments.

[0099] Effect Example 3 Desensitization Gel Stability Test

[0100] Purpose of the test:

[0101] The storage stability of the desensitizing gel prepared by the present invention was verified.

[0102] Test method:

[0103] The product properties and dynamic viscosity changes of the desensitizing gels prepared in Examples 1-4 of the present invention and Comparative Examples 1-3 were respectively detected after being stored at room temperature for 6 months and in a 42° C. oven for 6 months.

[0104] Test results:

[0105] Table 3 Desensitization gel stability test results

[0106]

[0107] Results analysis: Table 3 shows that after being stored at room temperature and 42°C respectively, Examples 1-4 and Comparative Examples 1-3 have better product properties and dynamic viscosity than the Comparative Examples, indicating that the desensitizing gel of the Examples is more stable than that of the Comparative Examples, and the properties do not change even when stored at 42°C. However, since the Comparative Examples do not fully implement the desensitizing gel preparation method of the present invention, the properties change after being stored at room temperature for 6 months. After being stored at 42°C, the product properties and dynamic viscosity change more significantly, showing greater instability. In the Examples, Example 3 has better dynamic viscosity, making it easier for the gel to cover the oral surface and exert multiple functions.

[0108] Unless otherwise specifically stated, the numerical value set forth in these embodiments does not limit the scope of the present invention. In all examples shown and described here, unless otherwise specified, any specific value should be interpreted as merely exemplary, rather than as restriction, and therefore, other examples of exemplary embodiments may have different values.

Claims

1. A method for preparing a multifunctional oral desensitization gel, the preparation method of which is as follows: Step 1: add the anti-allergic agent to 15%-20% purified water in proportion, stir and dissolve, adjust the solution pH to between 7 and 10 with 0.5-1M citric acid solution, add 3%-8% by mass of bioactive minerals to the above solution, treat with a 19-25kHZ ultrasonic disperser for 2-10 minutes, mix evenly, so that the anti-allergic agent can fully enter the porous structure of the bioactive minerals, and prepare component I; Step 2: Weigh 0.5-1% collagen peptide, 0.2-1% casein phosphopeptide, 0.1-1% antimicrobial peptide and cross-linking agent by mass, add 30-40% purified water, stir and dissolve, stir and disperse for 5-20 minutes, cross-link, and prepare component II; Step 3: Component I and component II are mixed, stirred at a low speed of 100-200 rpm at 50°C-60°C for 10-30 min to cross-link the bioactive minerals and peptides to obtain active microcarriers; Step 4: weigh 8-20% by mass of glycerol, 0.2-0.8% by mass of sodium carboxymethyl cellulose, 0.1-0.5% by mass of xanthan gum, and 0.1-0.5% by mass of guar gum, stir them evenly, and then slowly add them into the system of step 3, keep stirring at 50°C-60°C until the system is uniform, and the total heating time shall not exceed 2 hours to obtain a gel system; Step 5: Add other components to the gel system prepared in step 4: including 2-5% by mass of an abrasive, 0.1%-0.4% of a preservative, 0.01%-0.05% of an antioxidant, 0.2%-1.5% of an anti-caries agent and the remainder being purified water, to finally obtain a multifunctional oral desensitizing gel with a dynamic viscosity greater than 23000 mPa·s and a dental tubule blocking rate greater than 70%.

2. The method for preparing a multifunctional oral desensitizing gel according to claim 1, characterized in that: In step 1, the anti-allergic agent includes 0.5%-1.5% L-arginine and 0.1%-0.5% dipotassium glycyrrhizinate.

3. The method for preparing a multifunctional oral desensitizing gel according to claim 1, characterized in that: In step 1, the bioactive minerals include nano-hydroxyapatite, β-tricalcium phosphate, and nano-silicon dioxide, the mass ratio of which is 2:1:(0.1-0.5), the particle size of nano-hydroxyapatite is 200-500 nanometers; the particle size of nano-silicon dioxide is 50-100 nanometers, and the hydroxyapatite can be spherical or short rod-shaped.

4. The method for preparing a multifunctional oral desensitizing gel according to claim 1, characterized in that: In step 2, the molecular weight of collagen peptide is 10KDa-50KDa, the molecular weight of casein phosphopeptide is 2KDa-3KDa, the molecular weight of antimicrobial peptide is 2KDa-7KDa, and the cross-linking agent is 3-6% potassium citrate.

5. The method for preparing a multifunctional oral desensitizing gel according to claim 1, characterized in that: In step 5, the abrasive is silicon dioxide with a diameter of 10-20 microns; the preservative is one or more of sodium ethylparaben, sodium butylparaben, and ε-polylysine hydrochloride; the antioxidant is one or more of vitamin E, sodium phytate, and disodium ethylenediaminetetraacetic acid; and the anti-caries agent is one or more of erythritol, xylitol, and sorbitol liquid.

6. A multifunctional oral desensitizing gel, obtained by the preparation method according to any one of claims 1 to 5.

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