New application of Wuzi Yanzong pill
Through research, Wuzi Yanzong Wan does not need to add taste, which can effectively reduce blood sugar, improve diabetic neuropathy, and prevent Alzheimer's disease. It solves the problem of adding taste in the existing technology to achieve the treatment effect and expand its application scope.
Patent Information
- Application Number
- CN202310920998.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-07-25
- Publication Date
- 2025-01-28
AI Technical Summary
In the prior art, Wuzi Yanzong Pills need to be added to the odor when treating diabetes and Alzheimer's disease, and cannot effectively reduce blood sugar or improve diabetic neuropathy. Moreover, their anti-dementia and neuroprotective effects are not possessed by Wuzi Yanzong Pills themselves.
Through research, Wuzi Yanzong Wan does not need to add taste, which can effectively reduce blood sugar, improve diabetic neuropathy, and have the effect of preventing and controlling Alzheimer's disease.
Wuzi Yanzong Wan can reduce blood sugar, improve diabetic neuropathy without taste, and prevent Alzheimer's disease, and expand its application scope.
Smart Images
Figure CN119367440A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of new uses of traditional Chinese medicines, and in particular to a new use of Wuzi Yanzong Pills in the preparation of drugs for lowering blood sugar, improving diabetic neuropathy or preventing and treating Alzheimer's disease. Background Art
[0002] Wuzi Yanzong Pills are composed of wolfberry, dodder seed, raspberry, schisandra chinensis, and plantain seed. It is a classic prescription in traditional Chinese medicine for tonifying the kidney, consolidating sperm and assisting kidney yang. It has the effects of tonifying the kidney and strengthening yang, improving sperm quality, preventing neural tube defects, protecting the normal development of the embryo, improving endometrial receptivity and promoting immunity. It is widely used in the treatment of reproductive system diseases.
[0003] Wu Zi Yan Zong Pills were originally designed to nourish the kidney and replenish essence. Under the guidance of TCM's view on syndrome differentiation and treatment and the idea of "different diseases treated with the same method", all symptoms of kidney deficiency and essence deficiency can be treated with Wu Zi Yan Zong Pills. However, usually it is necessary to add flavors to the Wu Zi Yan Zong Pills for treatment.
[0004] For example, diabetes belongs to the category of "polyuria" in traditional Chinese medicine, and its pathogenesis is closely related to the kidney. Zhang Zhongjing proposed that "polyuria" should be treated from the perspective of the kidney. The "Golden Chamber" says: "Men with polyuria will urinate more frequently. Drink one dou of wine and urinate one dou of wine. Shenqi Pills are the main treatment." However, the prior art discloses the treatment of diabetes with Wuzi Yanzong Pills. That is, the existing literature records that it is necessary to add herbs such as Strychnos nux vomica, Angelica sinensis, and Pheretima lumbricoides to Wuzi Yanzong Pills, and then combine them with acupoint injection for combined treatment to effectively achieve the purpose of lowering blood sugar. Based on the teachings of the prior art, technicians in this field cannot reasonably expect that only Wuzi Yanzong Pills can have a clear therapeutic effect on diabetes, let alone that only Wuzi Yanzong Pills can effectively improve diabetic neuropathy.
[0005] Similarly, whether a person's memory is normal or not is closely related to whether the kidney's essence is full or not; "Yi Fang Ji Jie·Nourishment Agent" says, "If the kidney essence is insufficient, the will and energy will decline, and it cannot reach the heart, so it is confused and forgetful." This shows that cognitive impairment is related to kidney deficiency and empty marrow. Sufficient kidney essence is the prerequisite for nourishing the brain marrow to maintain normal brain thinking and memory. However, the only medicine disclosed in the prior art that can play an anti-dementia and neuroprotective role is Jiawei Wuzi Yanzong Pills, which requires the addition of herbs such as Epimedium to the Wuzi Yanzong Pills to achieve the corresponding effects. Therefore, the above-mentioned anti-dementia and neuroprotective effects are not the uses of Wuzi Yanzong Pills themselves. Summary of the invention
[0006] Therefore, the present invention provides a new use of Wuzi Yanzong Pills, that is, a new use of Wuzi Yanzong Pills for achieving the effects of lowering blood sugar, improving diabetic neuropathy or preventing and treating Alzheimer's disease.
[0007] A use of Wuzi Yanzong Pills in preparing a drug for lowering blood sugar.
[0008] The blood sugar lowering drug is a drug that lowers blood sugar by lowering glucose levels.
[0009] A use of Wuzi Yanzong Pills in preparing a drug for improving diabetic neuropathy.
[0010] The drug for improving diabetic neuropathy is a drug that improves diabetic neuropathy by enhancing the fluorescence intensity of nerves and / or reducing the number of neutrophils around nerves.
[0011] A use of Wuzi Yanzong Pills in preparing a drug for preventing and treating Alzheimer's disease.
[0012] The drug for preventing and treating Alzheimer's disease is a drug that prevents and treats Alzheimer's disease by increasing reaction speed and movement distance, improving reaction ability and restoring motor dysfunction or inhibiting acetylcholinesterase activity.
[0013] The technical solution of the present invention has the following advantages:
[0014] 1. In the experimental research on Wuzi Yanzong Pills, the present invention found that there is no need to add flavor to Wuzi Yanzong Pills. Only Wuzi Yanzong Pills can also have the effect of lowering blood sugar and improving diabetic neuropathy, which overcomes the technical prejudice of those skilled in the art that flavoring is needed to achieve the corresponding effect. At the same time, there is a close connection between diabetes and Alzheimer's disease. The present invention also verifies that Wuzi Yanzong Pills have the effect of preventing and treating Alzheimer's disease, can improve plaque reaction ability and restore motor dysfunction, and inhibit related enzyme activities. Therefore, the present invention provides a new use of Wuzi Yanzong Pills, which includes the use of lowering blood sugar, improving diabetic neuropathy or preventing and treating Alzheimer's disease, etc., which greatly expands the scope of application of Wuzi Yanzong Pills.
[0015] 2. Wuzi Yanzong Pills are originally designed to nourish the kidney and replenish essence. Based on the idea of "different diseases are treated with the same method" in traditional Chinese medicine, the present invention provides a new use of Wuzi Yanzong Pills. The new application of Wuzi Yanzong Pills is explored using the model animal zebrafish, which can effectively evaluate the efficacy, safety and other indicators of the drug. Experiments have shown that Wuzi Yanzong Pills have the effects of lowering blood sugar, protecting peripheral nerves, relieving peripheral nerve inflammation, improving reaction ability, restoring motor dysfunction, and inhibiting acetylcholinesterase activity, proving its application in drugs for lowering blood sugar and improving diabetic neuropathy, and in drugs for preventing and treating Alzheimer's disease. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0017] Figure 1 is a microscopic photograph of the fluorescence intensity of zebrafish nerves in the transgenic motor neuron green fluorescent NBT strain of Example 1 of the present invention;
[0018] Figure 2 This is a statistical analysis result diagram of the fluorescence intensity of zebrafish nerves in the transgenic motor neuron green fluorescence NBT strain of Example 1 of the present invention;
[0019] Figure 3 is a microscopic photograph of zebrafish perineuronal neutrophils in the hybrid strain of Example 1 of the present invention;
[0020] Figure 4 is a diagram showing the statistical analysis results of zebrafish perineuronal neutrophils in the hybrid strain of Example 1 of the present invention;
[0021] Figure 5 is a statistical analysis result diagram of the movement speed of the wild-type AB strain zebrafish of Example 2 of the present invention;
[0022] Figure 6 is a statistical analysis result diagram of the movement distance of the wild-type AB strain zebrafish of Example 2 of the present invention;
[0023] Figure 7 This is a typical diagram of the movement trajectory of the wild-type AB strain zebrafish in Example 2 of the present invention;
[0024] Figure 8 This is a statistical analysis result diagram of the fluorescence value of acetylcholinesterase (AchE) of wild-type AB strain zebrafish in Example 2 of the present invention. DETAILED DESCRIPTION
[0025] Example 1
[0026] A use of Wuzi Yanzong Pills in the preparation of drugs for lowering blood sugar and drugs for improving diabetic neuropathy. The effects thereof are verified as follows:
[0027] 1.1 Model preparation, grouping and drug administration
[0028] The zebrafish hyperglycemia model was established by administering 1.5 mg / mL of water-soluble egg yolk powder solution during the day and 30 mg / mL of water-soluble glucose solution at night. The zebrafish hyperglycemia model was randomly divided into a model control group, a positive control group, a low-dose Wuzi Yanzong Pill group, a medium-dose Wuzi Yanzong Pill group, and a high-dose Wuzi Yanzong Pill group. The group that was not administered with water-soluble egg yolk powder solution and water-soluble glucose solution was used as a normal control group; 30 fish in each group. Among them, the model group was not administered with any drug, the positive control group was administered with a specific concentration and type of positive drug in water, the dosage of the low-dose Wuzi Yanzong Pill group in water was 500 μg / mL, the dosage of the medium-dose Wuzi Yanzong Pill group was 1000 μg / mL, and the dosage of the high-dose Wuzi Yanzong Pill group was 2000 μg / mL. The positive drug for the evaluation of blood sugar level was pioglitazone hydrochloride (20.0 μg / mL), and the positive drug for the evaluation of peripheral nerve protection efficacy and peripheral nerve inflammation reduction efficacy was sitagliptin phosphate (350 μg / mL).
[0029] 1.2 Effects on Glucose Levels
[0030] Wild-type AB strain zebrafish 5 days after fertilization (5dpf) were randomly selected, and the model was prepared and grouped in the manner described in 1.1. Specifically, after the zebrafish hyperglycemia model was established, it was divided into a normal control group, a model control group, a positive control group of pioglitazone hydrochloride, a low-dose group of Wuzi Yanzong Pills, a medium-dose group of Wuzi Yanzong Pills, and a high-dose group of Wuzi Yanzong Pills. After grouping, at 28°C, the fluid was changed and administered every morning and evening according to the dosage in 1.1. After 48 hours of administration, the zebrafish was washed 3 times with water, and the overall glucose level of the zebrafish was detected with a blood glucose meter.
[0031] 1.3 Evaluation of peripheral nerve protection efficacy
[0032] 5dpf transgenic motor neuron green fluorescent NBT strain zebrafish were randomly selected, and the model was prepared and grouped in the manner described in 1.1. Specifically, after the zebrafish hyperglycemia model was established, it was divided into a normal control group, a model control group, a positive control group of sitagliptin phosphate, a low-dose group of Wuzi Yanzong Pills, a medium-dose group of Wuzi Yanzong Pills, and a high-dose group of Wuzi Yanzong Pills. After grouping, at 28°C, the fluid was changed and administered every morning and evening according to the dosage in 1.1. After 4 days of administration, 10 zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. The statistical analysis results of the zebrafish nerve fluorescence intensity were used to evaluate the peripheral nerve protective efficacy of Wuzi Yanzong Pills.
[0033] 1.4 Evaluation of efficacy in relieving peripheral neuritis
[0034] Randomly select 5dpf transgenic neutrophil fluorescence zebrafish strains and peripheral motor nerve green fluorescence zebrafish hybrid strain zebrafish, and prepare and group the model in the manner described in 1.1. Specifically, after the zebrafish hyperglycemia model was established, it was divided into a normal control group, a model control group, a positive control group of sitagliptin phosphate, a low-dose group of Wuzi Yanzong Pills, a medium-dose group of Wuzi Yanzong Pills, and a high-dose group of Wuzi Yanzong Pills. After grouping, at 28°C, the fluid was changed and administered every morning and evening according to the dosage in 1.1. After 4 days of administration, 10 zebrafish were randomly selected from each group and photographed under a fluorescence microscope. The statistical analysis results of the number of neutrophils around the zebrafish nerves were used to evaluate the efficacy of Wuzi Yanzong Pills in reducing peripheral nerve inflammation.
[0035] 1.5 Experimental Results
[0036] The results of the effects on glucose levels are shown in Table 1, and the results of the evaluation of peripheral nerve protective efficacy are shown in Table 1. Figure 1 and Figure 2 The results of the evaluation of the efficacy of peripheral neuritis reduction are shown in Figure 3 and Figure 4 shown.
[0037] Table 1 Effect of Wuzi Yanzong Pills on glucose levels
[0038]
[0039] Compared with the model control group, **p<0.01, ***p<0.001.
[0040] As shown in Table 1 above, after zebrafish were treated with egg yolk powder and glucose, the glucose level increased significantly, indicating that the model was successfully established. Both the positive control group and the Wuzi Yanzong Pill group could reduce the glucose level of zebrafish.
[0041] Diabetic neuropathy is the most common chronic complication of diabetes, and patients with a course of disease of more than 10 years are prone to obvious clinical manifestations of neuropathy. Figure 1 and Figure 2 As shown, 10 zebrafish were randomly selected and photographed under a fluorescence microscope, and the fluorescence intensity of zebrafish nerves and the number of neutrophils around the nerves were counted. In the transgenic motor neuron green fluorescence NBT strain, the nerve fluorescence intensity of the model group was reduced, and the nerve fluorescence intensity of the Wuzi Yanzong Pill group was enhanced compared with the model group, indicating that Wuzi Yanzong Pill has the effect of protecting peripheral nerves.
[0042] like Figure 3 and Figure 4As shown, in 10 randomly selected transgenic neutrophil fluorescent zebrafish strains and peripheral motor nerve green fluorescent zebrafish hybrid strains, the number of neutrophils around the nerves in the model group was enhanced, while the number of neutrophils around the nerves in the Wuzi Yanzong Pills group was lower than that in the model group, indicating that Wuzi Yanzong Pills has the effect of resolving peripheral nerve inflammation.
[0043] Example 2
[0044] A use of Wuzi Yanzong Pills in the preparation of a drug for preventing and treating Alzheimer's disease. The effects thereof are verified as follows:
[0045] 2.1 Model preparation, grouping and drug administration
[0046] The zebrafish Alzheimer's disease model was established by administering water-soluble aluminum chloride hexahydrate solution. The zebrafish were randomly divided into a normal control group, a model control group, a positive group, and a Wuzi Yanzong pill group, with 30 fish in each group. The drug-administered groups were exposed to the corresponding test drugs in water-soluble form; specifically, the normal control group and the model control group were not given drugs, the test drug administered to the positive group was donepezil hydrochloride (3.33 μg / mL), and the test drug administered to the Wuzi Yanzong pill group was Wuzi Yanzong pill, which were 500 μg / mL low-dose group, 1000 μg / mL medium-dose group, and 2000 μg / mL high-dose group.
[0047] 2.2 Evaluation of recovery of reaction ability and motor dysfunction
[0048] 4dpf wild-type AB strain zebrafish were randomly selected, and the model was prepared and grouped in the manner described in 2.1. Specifically, after the zebrafish Alzheimer's disease model was established, it was divided into a normal control group, a model control group, a positive control group of donepezil hydrochloride, a low-dose group of Wuzi Yanzong Pills, a medium-dose group of Wuzi Yanzong Pills, and a high-dose group of Wuzi Yanzong Pills. After grouping, the drug was administered once at 28°C according to the dosage in 2.1. 24 hours after administration, 10 zebrafish were randomly selected from each experimental group. The statistical analysis results of the average movement speed per minute of zebrafish under light and dark stimulation were used to evaluate the reaction ability recovery effect of Wuzi Yanzong Pills, and the statistical analysis results of the movement distance of zebrafish were used to evaluate the motor dysfunction recovery effect of Wuzi Yanzong Pills.
[0049] 2.3 Evaluation of acetylcholinesterase inhibition
[0050] 4dpf wild-type AB strain zebrafish were randomly selected, and the model was prepared and grouped in the manner described in 2.1. Specifically, after the zebrafish Alzheimer's disease model was established, it was divided into a normal control group, a model control group, a positive control group of donepezil hydrochloride, a low-dose group of Wuzi Yanzong Pills, a medium-dose group of Wuzi Yanzong Pills, and a high-dose group of Wuzi Yanzong Pills. After grouping, the drug was administered once at 28°C according to the dosage in 2.1. After 48 hours of administration, the acetylcholinesterase assay kit was used to collect data using a multifunctional microplate reader, and the statistical analysis results of the acetylcholinesterase fluorescence value in zebrafish were used to evaluate the inhibitory effect of the sample on acetylcholinesterase.
[0051] 2.4 Experimental Results
[0052] The results of the evaluation of the recovery of reaction ability and motor dysfunction are as follows Figure 5-Figure 7 The results of the evaluation of acetylcholinesterase inhibition are shown in Figure 8 shown.
[0053] like Figure 5-7 As shown, after zebrafish were treated with aluminum chloride hexahydrate, the average movement speed per minute and the movement distance of zebrafish under light and dark stimulation decreased. Compared with the model group, the positive control group and Wuzi Yanzong Pills group could increase the reaction speed and movement distance of zebrafish, indicating that Wuzi Yanzong Pills can improve reaction ability and restore motor dysfunction.
[0054] like Figure 8 As shown in the figure, the fluorescence values of acetylcholinesterase in zebrafish under different treatment groups were analyzed. There was no significant difference between the low and medium concentrations of Wuzi Yanzong Pills and the model group, while the high concentration group could significantly reduce the level of acetylcholinesterase, indicating that Wuzi Yanzong Pills may achieve the effect of preventing and treating Alzheimer's disease by inhibiting the activity of acetylcholinesterase.
[0055] In summary, Wuzi Yanzong Pills have a good effect in lowering blood sugar and improving diabetic neuropathy, and can prevent and treat Alzheimer's disease.
[0056] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.
Claims
1. Use of Wuzi Yanzong Pills in preparing a drug for lowering blood sugar.
2. The use according to claim 1, wherein the blood sugar lowering drug is a drug that lowers blood sugar by lowering glucose levels.
3. Use of Wuzi Yanzong Pills in the preparation of a drug for improving diabetic neuropathy.
4. The use according to claim 3, wherein the drug for improving diabetic neuropathy is a drug that improves diabetic neuropathy by enhancing the intensity of nerve fluorescence and / or reducing the number of neutrophils around nerves.
5. Use of Wuzi Yanzong Pills in preparing drugs for preventing and treating Alzheimer's disease.
6. The use according to claim 5, wherein the drug for preventing and treating Alzheimer's disease is a drug for preventing and treating Alzheimer's disease by increasing reaction speed and movement distance, improving reaction ability and restoring motor dysfunction or inhibiting acetylcholinesterase activity.