A high-efficiency domestic sewage treatment agent and preparation method thereof

Abstract

The invention discloses a highly efficient domestic sewage treatment agent and a preparation method thereof, and belongs to the technical field of sewage treatment. The sewage treatment agent is made of 60-80 parts of a composite material, 20-40 parts of modified montmorillonite, and 30-50 parts of a composite microbial bacterial solution; the composite microbial bacterial solution is made of Bacillus coagulans, Bacillus cladodes, and Trichoderma viride; the composite material is an iron-based biochar composite material; the modified montmorillonite is a humic acid modified montmorillonite. The water treatment agent uses a combination of physical adsorption, chemical degradation, and biological treatment to deeply purify domestic sewage, which can effectively improve the removal rates of COD, BOD, and nitrogen and phosphorus, purify water quality, and improve water transparency, and has good market application prospects.

Description

Technical Field

[0001] The invention belongs to the technical field of sewage treatment, and specifically relates to a high-efficiency domestic sewage treatment agent and a preparation method thereof. Background Art

[0002] Domestic sewage refers to wastewater generated by people in their daily lives. It mainly comes from residential areas, public places, government agencies, schools, hospitals, shops, factory living rooms and other places, including water discharged from toilets, bathrooms, kitchens, laundry rooms, etc. These sewage contain organic substances such as protein, animal fat, carbohydrates, urea, ammonia nitrogen, as well as impurities such as soap, detergents, pathogenic microorganisms, and parasite eggs. If domestic sewage is not properly treated, it will have a multi-faceted harmful impact on residents' lives. For example, domestic sewage contains a large number of pathogenic microorganisms, such as bacteria, viruses, and parasite eggs; these pathogens can enter the human body through various channels and survive in the body, causing various diseases, such as intestinal infectious diseases, skin diseases, etc., which seriously endanger human health. At the same time, domestic sewage contains a large amount of plant nutrients such as nitrogen and phosphorus. Excessive amounts of these substances will lead to eutrophication of water bodies. Eutrophication will cause algae to multiply, consume oxygen in the water, further deteriorate the water quality, and in severe cases, lead to the collapse of aquatic ecosystems. If domestic sewage is discharged directly without treatment or is not treated thoroughly, it will seep into the groundwater system and pollute the groundwater. Groundwater is an important source of drinking water for people. Once polluted, it will seriously threaten the drinking water safety of residents.

[0003] In order to reduce the harmful effects of domestic sewage on residents' lives, a series of measures need to be taken to treat sewage and manage its discharge. At present, the main methods for sewage treatment are chemical and biological methods. The chemical method is to achieve the effect of water purification by adding drugs to the water body, but due to the complex types of pollutants in sewage, some existing water treatment agents are difficult to synergistically treat multiple pollutants such as ammonia nitrogen, COD and heavy metal ions, and the effect of sewage purification is poor; for the biological method, a certain amount of microbial agents are added to the water body multiple times to achieve the purification effect, but this kind of microorganism directly added to the water body exists in a free form, resulting in its serious loss and inability to act in the water body for a long time, thereby limiting the purification effect on sewage. Therefore, how to prepare a sewage treatment agent with higher activity and stability to improve the efficiency and purification effect of domestic sewage treatment has become a technical problem that needs to be solved urgently. Summary of the invention

[0004] The purpose of the present invention is to provide an efficient domestic sewage treatment agent, which uses a combination of physical adsorption-chemical degradation-biological treatment to deeply purify domestic sewage, can effectively improve the COD, BOD and nitrogen and phosphorus removal rates, purify water quality, and improve water transparency.

[0005] In order to achieve the above technical objectives, the technical solution adopted by the present invention is:

[0006] A highly efficient domestic sewage treatment agent is made of the following raw materials in parts by weight: 60-80 parts of composite material, 20-40 parts of modified montmorillonite, and 30-50 parts of composite microbial liquid; the composite microbial liquid is made of Bacillus coagulans ( Bacillus )、Bacillus ( Virgibacillus )、Trichoderma viride( Trichoderma ); the Bacillus coagulans strain is numbered CCTCC CB 20082253, the preservation date is 2005-09-27, and it is purchased from China Center for Type Culture Collection; the Bacillus cladodes strain is numbered CCTCC AA 207023, the preservation date is 2007-05-23, and it is purchased from China Center for Type Culture Collection; the viride strain is numbered CCTCC CF 2008706, the preservation date is 2005-10-24, and it is purchased from China Center for Type Culture Collection; the composite material is an iron-based-biochar composite material; and the modified montmorillonite is humic acid modified montmorillonite.

[0007] The Bacillus coagulans, Bacillus cladocerus and Trichoderma viride used in the present invention can be purchased without any worries through the open catalog of the preservation center, without the need for repeated preservation.

[0008] Preferably, the composite material is prepared by the following method:

[0009] (1) Add 0.5 mol / L FeCl 3 Solution and 0.2 mol / L ZnCl 2 The solutions are mixed in equal proportions to obtain a mixed solution A;

[0010] (2) Disperse 2 g of modified biochar in 100 ml of deionized water and ultrasonically disperse for 30-40 min to obtain suspension B;

[0011] (3) Heat a 3 mol / L NaOH solution to 75-85°C, then slowly add the suspension B therein, add the mixed solution A therein after the addition is complete, and continue to react with magnetic stirring for 3-4 hours. After the reaction is completed, wash the solid product to neutrality by solid-liquid separation, and vacuum dry to obtain the composite material.

[0012] Preferably, the modified biochar is prepared by the following method: straw biochar and nano cuprous oxide are mixed in a mass ratio of 100:2-3, dispersed in deionized water, ultrasonically dispersed for 10 minutes, and then an appropriate amount of NaOH solution is added. The mixture is magnetically stirred at a speed of 150 r / min at room temperature for 10-12 hours, allowed to stand for 48 hours, filtered, and the solid is washed with deionized water until neutral. After drying, it is transferred to a muffle furnace and calcined at high temperature for 2 hours to obtain the modified biochar.

[0013] Preferably, in step (3), the volume ratio of the NaOH solution, the mixed solution A and the suspension B is 3:1:1.

[0014] Preferably, the high-temperature calcination is carried out by heating the temperature to 500°C at a rate of 5°C / min and then calcining for 2h; the concentration of the NaOH solution is 0.1mmol / L, and the volume ratio of the NaOH solution to deionized water is 1:4; the mass of the deionized water is 3 times the mass of the straw biochar.

[0015] Preferably, the modified montmorillonite is prepared by the following method:

[0016] a) adding 10 g of montmorillonite powder to 200 ml of an ethanol-water mixture, adding 0.5 g of 3-aminopropyltriethoxysilane thereto, heating and reflux at 80° C. for 10-12 h, then performing solid-liquid separation to obtain a solid product, washing the solid with an ethanol-water mixture for 3-5 times, and vacuum drying to obtain silane-modified montmorillonite; the volume ratio of ethanol to deionized water in the ethanol-water mixture is 4:1;

[0017] b) Mixing silane-modified montmorillonite and humic acid in a mass ratio of 2:1, adding the mixture to 3 times the amount of deionized water, and ultrasonically dispersing the mixture for 10-15 minutes to obtain a suspension. Adjusting the pH of the suspension to 8.5, and reacting the mixture at 40-45° C. and 200 r / min for 24 hours with magnetic stirring. After the reaction, freeze-drying the mixture to obtain the modified montmorillonite.

[0018] Preferably, the composite microbial bacterial liquid is prepared by the following method: Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600 =2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain three fermentation broths, and then mix them in a volume ratio of 1:1:1 to obtain a composite microbial culture liquid.

[0019] Preferably, the fermentation liquid medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, MgSO 4 0.05g, KH 2 PO 4 0.02g, distilled water 1000ml.

[0020] Preferably, the LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, the pH is adjusted to 7.0; and steam sterilized at 121°C for 20 min.

[0021] The present invention also provides a method for preparing the above-mentioned high-efficiency domestic sewage treating agent, which comprises the following steps:

[0022] S1: preparing composite microbial culture liquid;

[0023] S2: Preparation of modified montmorillonite;

[0024] S3: The modified montmorillonite and the composite microbial liquid are mixed in proportion, stirred and mixed evenly, cultured at 30° C. for 2-3 days, and the mixture is freeze-dried in vacuum to obtain a mixed powder;

[0025] S4: Evenly mix the mixed powder with the prepared composite material to obtain a high-efficiency domestic sewage treatment agent.

[0026] The specific usage and dosage of the high-efficiency domestic sewage treatment agent of the present invention are: add the sewage treatment agent to the sewage to be treated, the dosage of the sewage treatment agent per cubic meter to be treated is 20-50g, stir the sewage treatment agent evenly after adding, irradiate with a 300W xenon lamp at room temperature for 2 hours, and then separate the solid and liquid.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] (1) The composite material of the present invention is a composite of magnetic nanoparticles and modified biochar. The magnetic nanoparticles have excellent catalytic activity, and the modified biochar is modified by doping with nano-cuprous oxide. The biochar itself has a porous structure and a large specific surface area, and can adsorb a variety of pollutants in sewage, including heavy metal ions, organic pollutants, etc., and the adsorption performance of the biochar doped with nano-cuprous oxide is further improved. At the same time, it can cooperate with the magnetic nanoparticles to improve the catalytic efficiency of the composite material. The present invention combines the catalytic effect of magnetic nanoparticles with the adsorption effect of modified biochar. The two promote each other, and can achieve rapid degradation and efficient removal of organic pollutants in sewage, thereby improving the efficiency of sewage treatment.

[0029] (2) The present invention screens three strains to prepare a composite microbial liquid, among which Bacillus coagulans can produce antibacterial substances such as antibacterial coagulants and lactic acid, which help to inhibit the growth of pathogens in sewage; Bacillus cladodes can promote the transformation and removal of nutrients such as nitrogen and phosphorus in sewage, which helps to improve water quality; and Trichoderma viride can produce a variety of enzyme substances, such as cellulase and ligninase, which can decompose the difficult-to-degrade organic matter in sewage. The three strains can cooperate with each other in sewage to decompose organic matter, transform nutrients and inhibit the growth of harmful microorganisms. This synergistic effect makes the microbial liquid have stronger adaptability and stability when treating sewage.

[0030] (3) The modified montmorillonite with humic acid is used in combination with the composite microbial solution. The modified montmorillonite can adsorb harmful substances in sewage, reduce the burden on the microbial solution, and enable it to decompose organic matter more efficiently. The montmorillonite modified with humic acid can not only enhance the adsorption capacity of montmorillonite for harmful substances such as heavy metal ions and organic pollutants in sewage, but also provide nutrients and growth environment for microorganisms, promote the reproduction and metabolic activities of microorganisms, and improve the treatment effect of microorganisms on sewage. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a scanning electron microscope image of the appearance of the composite material prepared in the embodiment of the present invention, wherein a is the straw biochar raw material, b is the modified biochar, and c is the composite material;

[0032] Figure 2 This is a comparison diagram of sewage before and after being treated with the high-efficiency domestic sewage treatment agent of the present invention. DETAILED DESCRIPTION

[0033] The technical scheme of the present invention is further described below in conjunction with specific embodiments, but is not limited thereto. The Bacillus coagulans strain used in this embodiment is numbered CCTCC CB 20082253, deposited on 2005-09-27, and purchased from China Center for Type Culture Collection; the Bacillus cladodes strain is numbered CCTCC AA 207023, deposited on 2007-05-23, and purchased from China Center for Type Culture Collection; the Trichoderma viride strain is numbered CCTCC CF 2008706, deposited on 2005-10-24, and purchased from China Center for Type Culture Collection.

[0034] Example 1

[0035] A high-efficiency domestic sewage treatment agent is made of the following raw materials in parts by weight: 60 kg of a composite material, 20 kg of modified montmorillonite, and 30 kg of a composite microbial liquid; the composite microbial liquid is made of Bacillus coagulans, Bacillus subtilis, and Trichoderma viride; the composite material is an iron-based-biochar composite material; and the modified montmorillonite is humic acid-modified montmorillonite.

[0036] The composite material is prepared by the following method:

[0037] (1) Add 0.5 mol / L FeCl 3 Solution and 0.2 mol / L ZnCl 2 The solutions are mixed in equal proportions to obtain a mixed solution A;

[0038] (2) Disperse 2 g of modified biochar in 100 ml of deionized water and ultrasonically disperse for 30-40 min to obtain suspension B;

[0039] (3) Heat 150 ml of 3 mol / L NaOH solution to 75-85°C, then slowly add 50 ml of suspension B, add 50 ml of mixed solution A after the addition is complete, and continue to react with magnetic stirring for 3-4 hours. After the reaction is completed, wash the solid product to neutrality by solid-liquid separation, and vacuum dry to obtain the composite material.

[0040] The modified biochar is prepared by the following method: 100g of straw biochar is mixed with 2-3g of nano cuprous oxide, dispersed in 300ml of deionized water, ultrasonically dispersed for 10min, and then 75ml of 0.1mmol / L NaOH solution is added, magnetically stirred at a speed of 150r / min at room temperature for 10-12h, allowed to stand for 48h, filtered, and the solid is washed with deionized water to neutrality, and after drying, transferred to a muffle furnace and heated to 500°C at a rate of 5°C / min, and then calcined for 2h to obtain the modified biochar.

[0041] The modified montmorillonite is prepared by the following method:

[0042] a) adding 10 g of montmorillonite powder to 200 ml of an ethanol-water mixture, adding 0.5 g of 3-aminopropyltriethoxysilane thereto, heating and reflux at 80° C. for 10-12 h, then performing solid-liquid separation to obtain a solid product, washing the solid with an ethanol-water mixture for 3-5 times, and vacuum drying to obtain silane-modified montmorillonite; the volume ratio of ethanol to deionized water in the ethanol-water mixture is 4:1;

[0043] b) Mixing silane-modified montmorillonite and humic acid in a mass ratio of 2:1, adding the mixture to 3 times the amount of deionized water, and ultrasonically dispersing the mixture for 10-15 minutes to obtain a suspension. Adjusting the pH of the suspension to 8.5, and reacting the mixture at 40-45°C and 200 r / min for 24 hours with magnetic stirring. After the reaction, freeze-drying the mixture to obtain the modified montmorillonite.

[0044] The composite microbial bacterial liquid is prepared by the following method: Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600 =2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain three fermentation broths, and then mix them in a volume ratio of 1:1:1 to obtain a composite microbial culture liquid.

[0045] The fermentation liquid medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, MgSO 4 0.05g, KH 2 PO 4 0.02g, distilled water 1000ml.

[0046] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0047] The preparation method of the above-mentioned high-efficiency domestic sewage treatment agent comprises the following steps:

[0048] S1: preparing composite microbial culture liquid;

[0049] S2: Preparation of modified montmorillonite;

[0050] S3: The modified montmorillonite and the composite microbial liquid are mixed in proportion, stirred and mixed evenly, cultured at 30° C. for 2-3 days, and the mixture is freeze-dried in vacuum to obtain a mixed powder;

[0051] S4: Evenly mix the mixed powder with the prepared composite material to obtain a high-efficiency domestic sewage treatment agent.

[0052] Example 2

[0053] A high-efficiency domestic sewage treatment agent is made of the following raw materials in parts by weight: 80 kg of a composite material, 40 kg of modified montmorillonite, and 50 kg of a composite microbial liquid; the composite microbial liquid is made of Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride; the composite material is an iron-based-biochar composite material; and the modified montmorillonite is humic acid-modified montmorillonite.

[0054] The composite material is prepared by the following method:

[0055] (1) Add 0.5 mol / L FeCl 3 Solution and 0.2 mol / L ZnCl 2 The solutions are mixed in equal proportions to obtain a mixed solution A;

[0056] (2) Disperse 2 g of modified biochar in 100 ml of deionized water and ultrasonically disperse for 30-40 min to obtain suspension B;

[0057] (3) Heat 150 ml of 3 mol / L NaOH solution to 75-85°C, then slowly add 50 ml of suspension B, add 50 ml of mixed solution A after the addition is complete, and continue to react with magnetic stirring for 3-4 hours. After the reaction is completed, wash the solid product to neutrality by solid-liquid separation, and vacuum dry to obtain the composite material.

[0058] The modified biochar is prepared by the following method: 100g of straw biochar is mixed with 2-3g of nano cuprous oxide, dispersed in 300ml of deionized water, ultrasonically dispersed for 10min, and then 75ml of 0.1mmol / L NaOH solution is added, magnetically stirred at a speed of 150r / min at room temperature for 10-12h, allowed to stand for 48h, filtered, and the solid is washed with deionized water to neutrality, and after drying, transferred to a muffle furnace and heated to 500°C at a rate of 5°C / min, and then calcined for 2h to obtain the modified biochar.

[0059] The modified montmorillonite is prepared by the following method:

[0060] a) adding 10 g of montmorillonite powder to 200 ml of an ethanol-water mixture, adding 0.5 g of 3-aminopropyltriethoxysilane thereto, heating and reflux at 80° C. for 10-12 h, then performing solid-liquid separation to obtain a solid product, washing the solid with an ethanol-water mixture for 3-5 times, and vacuum drying to obtain silane-modified montmorillonite; the volume ratio of ethanol to deionized water in the ethanol-water mixture is 4:1;

[0061] b) Mixing silane-modified montmorillonite and humic acid in a mass ratio of 2:1, adding the mixture to 3 times the amount of deionized water, and ultrasonically dispersing the mixture for 10-15 minutes to obtain a suspension. Adjusting the pH of the suspension to 8.5, and reacting the mixture at 40-45°C and 200 r / min for 24 hours with magnetic stirring. After the reaction, freeze-drying the mixture to obtain the modified montmorillonite.

[0062] The composite microbial bacterial liquid is prepared by the following method: Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600 =2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain three fermentation broths, and then mix them in a volume ratio of 1:1:1 to obtain a composite microbial culture liquid.

[0063] The fermentation liquid medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, MgSO 4 0.05g, KH 2 PO 4 0.02g, distilled water 1000ml.

[0064] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0065] The preparation method of the above-mentioned high-efficiency domestic sewage treatment agent comprises the following steps:

[0066] S1: preparing composite microbial culture liquid;

[0067] S2: Preparation of modified montmorillonite;

[0068] S3: The modified montmorillonite and the composite microbial liquid are mixed in proportion, stirred and mixed evenly, cultured at 30° C. for 2-3 days, and the mixture is freeze-dried in vacuum to obtain a mixed powder;

[0069] S4: Evenly mix the mixed powder with the prepared composite material to obtain a high-efficiency domestic sewage treatment agent.

[0070] Example 3

[0071] A high-efficiency domestic sewage treatment agent is made of the following raw materials in parts by weight: 70 kg of a composite material, 30 kg of modified montmorillonite, and 40 kg of a composite microbial liquid; the composite microbial liquid is made of Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride; the composite material is an iron-based-biochar composite material; and the modified montmorillonite is humic acid-modified montmorillonite.

[0072] The composite material is prepared by the following method:

[0073] (1) Add 0.5 mol / L FeCl 3 Solution and 0.2 mol / L ZnCl 2 The solutions are mixed in equal proportions to obtain a mixed solution A;

[0074] (2) Disperse 2 g of modified biochar in 100 ml of deionized water and ultrasonically disperse for 30-40 min to obtain suspension B;

[0075] (3) Heat 150 ml of 3 mol / L NaOH solution to 75-85°C, then slowly add 50 ml of suspension B, add 50 ml of mixed solution A after the addition is complete, and continue to react with magnetic stirring for 3-4 hours. After the reaction is completed, wash the solid product to neutrality by solid-liquid separation, and vacuum dry to obtain the composite material.

[0076] The modified biochar is prepared by the following method: 100g of straw biochar is mixed with 2-3g of nano cuprous oxide, dispersed in 300ml of deionized water, ultrasonically dispersed for 10min, and then 75ml of 0.1mmol / L NaOH solution is added, magnetically stirred at a speed of 150r / min at room temperature for 10-12h, allowed to stand for 48h, filtered, and the solid is washed with deionized water to neutrality, and after drying, transferred to a muffle furnace and heated to 500°C at a rate of 5°C / min, and then calcined for 2h to obtain the modified biochar.

[0077] The modified montmorillonite is prepared by the following method:

[0078] a) adding 10 g of montmorillonite powder to 200 ml of an ethanol-water mixture, adding 0.5 g of 3-aminopropyltriethoxysilane thereto, heating and reflux at 80° C. for 10-12 h, then performing solid-liquid separation to obtain a solid product, washing the solid with an ethanol-water mixture for 3-5 times, and vacuum drying to obtain silane-modified montmorillonite; the volume ratio of ethanol to deionized water in the ethanol-water mixture is 4:1;

[0079] b) Mixing silane-modified montmorillonite and humic acid in a mass ratio of 2:1, adding the mixture to 3 times the amount of deionized water, and ultrasonically dispersing the mixture for 10-15 minutes to obtain a suspension. Adjusting the pH of the suspension to 8.5, and reacting the mixture at 40-45°C and 200 r / min for 24 hours with magnetic stirring. After the reaction, freeze-drying the mixture to obtain the modified montmorillonite.

[0080] The composite microbial bacterial liquid is prepared by the following method: Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600 =2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain three fermentation broths, and then mix them in a volume ratio of 1:1:1 to obtain a composite microbial culture liquid.

[0081] The fermentation liquid medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, MgSO 4 0.05g, KH 2 PO 4 0.02g, distilled water 1000ml.

[0082] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0083] The preparation method of the above-mentioned high-efficiency domestic sewage treatment agent comprises the following steps:

[0084] S1: preparing composite microbial culture liquid;

[0085] S2: Preparation of modified montmorillonite;

[0086] S3: The modified montmorillonite and the composite microbial liquid are mixed in proportion, stirred and mixed evenly, cultured at 30° C. for 2-3 days, and the mixture is freeze-dried in vacuum to obtain a mixed powder;

[0087] S4: Evenly mix the mixed powder with the prepared composite material to obtain a high-efficiency domestic sewage treatment agent.

[0088] Comparative Example 1

[0089] A high-efficiency domestic sewage treatment agent is made of the following raw materials in parts by weight: 70 kg of a composite material, 30 kg of modified montmorillonite, and 40 kg of a composite microbial liquid; the composite microbial liquid is made of Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride; the composite material is an iron-based-biochar composite material; and the modified montmorillonite is humic acid-modified montmorillonite.

[0090] The composite material is prepared by the following method:

[0091] (1) Add 0.5 mol / L FeCl 3 Solution and 0.2 mol / L ZnCl 2 The solutions are mixed in equal proportions to obtain a mixed solution A;

[0092] (2) Disperse 2 g of straw biochar in 100 ml of deionized water and perform ultrasonic dispersion for 30-40 min to obtain suspension B;

[0093] (3) Heat 150 ml of 3 mol / L NaOH solution to 75-85°C, then slowly add 50 ml of suspension B, add 50 ml of mixed solution A after the addition is complete, and continue to react with magnetic stirring for 3-4 hours. After the reaction is completed, wash the solid product to neutrality by solid-liquid separation, and vacuum dry to obtain the composite material.

[0094] The modified montmorillonite is prepared by the following method:

[0095] a) adding 10 g of montmorillonite powder to 200 ml of an ethanol-water mixture, adding 0.5 g of 3-aminopropyltriethoxysilane thereto, heating and reflux at 80° C. for 10-12 h, then performing solid-liquid separation to obtain a solid product, washing the solid with an ethanol-water mixture for 3-5 times, and vacuum drying to obtain silane-modified montmorillonite; the volume ratio of ethanol to deionized water in the ethanol-water mixture is 4:1;

[0096] b) Mixing silane-modified montmorillonite and humic acid in a mass ratio of 2:1, adding the mixture to 3 times the amount of deionized water, and ultrasonically dispersing the mixture for 10-15 minutes to obtain a suspension. Adjusting the pH of the suspension to 8.5, and reacting the mixture at 40-45°C and 200 r / min for 24 hours with magnetic stirring. After the reaction, freeze-drying the mixture to obtain the modified montmorillonite.

[0097] The composite microbial bacterial liquid is prepared by the following method: Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600=2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain three fermentation broths, and then mix them in a volume ratio of 1:1:1 to obtain a composite microbial culture liquid.

[0098] The fermentation liquid medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, MgSO 4 0.05g, KH 2 PO 4 0.02g, distilled water 1000ml.

[0099] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0100] The preparation method of the above-mentioned high-efficiency domestic sewage treatment agent comprises the following steps:

[0101] S1: preparing composite microbial culture liquid;

[0102] S2: Preparation of modified montmorillonite;

[0103] S3: The modified montmorillonite and the composite microbial liquid are mixed in proportion, stirred and mixed evenly, cultured at 30° C. for 2-3 days, and the mixture is freeze-dried in vacuum to obtain a mixed powder;

[0104] S4: Evenly mix the mixed powder with the prepared composite material to obtain a high-efficiency domestic sewage treatment agent.

[0105] This comparative example is basically the same as Example 3, except that the composite material is prepared using unmodified straw biochar.

[0106] Comparative Example 2

[0107] A high-efficiency domestic sewage treatment agent is made of the following raw materials in parts by weight: 70 kg of modified biochar, 30 kg of modified montmorillonite, and 40 kg of composite microbial liquid; the composite microbial liquid is made of Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride; the composite material is an iron-based-biochar composite material; and the modified montmorillonite is humic acid modified montmorillonite.

[0108] The modified biochar is prepared by the following method: 100g of straw biochar is mixed with 2-3g of nano cuprous oxide, dispersed in 300ml of deionized water, ultrasonically dispersed for 10min, and then 75ml of 0.1mmol / L NaOH solution is added, magnetically stirred at a speed of 150r / min at room temperature for 10-12h, allowed to stand for 48h, filtered, and the solid is washed with deionized water to neutrality, and after drying, transferred to a muffle furnace and heated to 500°C at a rate of 5°C / min, and then calcined for 2h to obtain the modified biochar.

[0109] The modified montmorillonite is prepared by the following method:

[0110] a) adding 10 g of montmorillonite powder to 200 ml of an ethanol-water mixture, adding 0.5 g of 3-aminopropyltriethoxysilane thereto, heating and reflux at 80° C. for 10-12 h, then performing solid-liquid separation to obtain a solid product, washing the solid with an ethanol-water mixture for 3-5 times, and vacuum drying to obtain silane-modified montmorillonite; the volume ratio of ethanol to deionized water in the ethanol-water mixture is 4:1;

[0111] b) Mixing silane-modified montmorillonite and humic acid in a mass ratio of 2:1, adding the mixture to 3 times the amount of deionized water, and ultrasonically dispersing the mixture for 10-15 minutes to obtain a suspension. Adjusting the pH of the suspension to 8.5, and reacting the mixture at 40-45°C and 200 r / min for 24 hours with magnetic stirring. After the reaction, freeze-drying the mixture to obtain the modified montmorillonite.

[0112] The composite microbial bacterial liquid is prepared by the following method: Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600 =2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain three fermentation broths, and then mix them in a volume ratio of 1:1:1 to obtain a composite microbial culture liquid.

[0113] The fermentation liquid medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, MgSO 4 0.05g, KH 2 PO 4 0.02g, distilled water 1000ml.

[0114] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0115] The preparation method of the above-mentioned high-efficiency domestic sewage treatment agent comprises the following steps:

[0116] S1: preparing composite microbial culture liquid;

[0117] S2: Preparation of modified montmorillonite;

[0118] S3: The modified montmorillonite and the composite microbial liquid are mixed in proportion, stirred and mixed evenly, cultured at 30° C. for 2-3 days, and the mixture is freeze-dried in vacuum to obtain a mixed powder;

[0119] S4: Evenly mix the mixed powder with the prepared modified biochar to obtain a high-efficiency domestic sewage treatment agent.

[0120] Comparative Example 3

[0121] A high-efficiency domestic sewage treatment agent is made of the following raw materials in parts by weight: 70 kg of a composite material, 30 kg of montmorillonite, and 40 kg of a composite microbial liquid; the composite microbial liquid is made of Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride; and the composite material is an iron-based-biochar composite material.

[0122] The composite material is prepared by the following method:

[0123] (1) Add 0.5 mol / L FeCl 3 Solution and 0.2 mol / L ZnCl 2 The solutions are mixed in equal proportions to obtain a mixed solution A;

[0124] (2) Disperse 2 g of modified biochar in 100 ml of deionized water and ultrasonically disperse for 30-40 min to obtain suspension B;

[0125] (3) Heat 150 ml of 3 mol / L NaOH solution to 75-85°C, then slowly add 50 ml of suspension B, add 50 ml of mixed solution A after the addition is complete, and continue to react with magnetic stirring for 3-4 hours. After the reaction is completed, wash the solid product to neutrality by solid-liquid separation, and vacuum dry to obtain the composite material.

[0126] The modified biochar is prepared by the following method: 100g of straw biochar is mixed with 2-3g of nano cuprous oxide, dispersed in 300ml of deionized water, ultrasonically dispersed for 10min, and then 75ml of 0.1mmol / L NaOH solution is added, magnetically stirred at a speed of 150r / min at room temperature for 10-12h, allowed to stand for 48h, filtered, and the solid is washed with deionized water to neutrality, and after drying, transferred to a muffle furnace and heated to 500°C at a rate of 5°C / min, and then calcined for 2h to obtain the modified biochar.

[0127] The composite microbial bacterial liquid is prepared by the following method: Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600 =2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain three fermentation broths, and then mix them in a volume ratio of 1:1:1 to obtain a composite microbial culture liquid.

[0128] The fermentation liquid medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, MgSO 4 0.05g, KH 2 PO 4 0.02g, distilled water 1000ml.

[0129] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0130] The preparation method of the above-mentioned high-efficiency domestic sewage treatment agent comprises the following steps:

[0131] S1: preparing composite microbial culture liquid;

[0132] S2: Mixing montmorillonite and composite microbial liquid in proportion, stirring and mixing evenly, culturing at 30° C. for 2-3 days, and vacuum freeze-drying the mixture to obtain a mixed powder;

[0133] S3: Evenly mix the mixed powder with the prepared composite material to obtain a high-efficiency domestic sewage treatment agent.

[0134] This comparative example is basically the same as Example 3, except that unmodified montmorillonite is directly used without the corresponding preparation method steps.

[0135] Comparative Example 4

[0136] A high-efficiency domestic sewage treatment agent is made of the following raw materials in parts by weight: 70 kg of a composite material, 30 kg of modified montmorillonite, and 40 kg of a composite microbial liquid; the composite microbial liquid is made of Bacillus coagulans and Bacillus cladocerus; the composite material is an iron-based-biochar composite material; and the modified montmorillonite is humic acid-modified montmorillonite.

[0137] The composite material is prepared by the following method:

[0138] (1) Add 0.5 mol / L FeCl 3 Solution and 0.2 mol / L ZnCl 2 The solutions are mixed in equal proportions to obtain a mixed solution A;

[0139] (2) Disperse 2 g of modified biochar in 100 ml of deionized water and ultrasonically disperse for 30-40 min to obtain suspension B;

[0140] (3) Heat 150 ml of 3 mol / L NaOH solution to 75-85°C, then slowly add 50 ml of suspension B, add 50 ml of mixed solution A after the addition is complete, and continue to react with magnetic stirring for 3-4 hours. After the reaction is completed, wash the solid product to neutrality by solid-liquid separation, and vacuum dry to obtain the composite material.

[0141] The modified biochar is prepared by the following method: 100g of straw biochar is mixed with 2-3g of nano cuprous oxide, dispersed in 300ml of deionized water, ultrasonically dispersed for 10min, and then 75ml of 0.1mmol / L NaOH solution is added, magnetically stirred at a speed of 150r / min at room temperature for 10-12h, allowed to stand for 48h, filtered, and the solid is washed with deionized water to neutrality, and after drying, transferred to a muffle furnace and heated to 500°C at a rate of 5°C / min, and then calcined for 2h to obtain the modified biochar.

[0142] The modified montmorillonite is prepared by the following method:

[0143] a) adding 10 g of montmorillonite powder to 200 ml of an ethanol-water mixture, adding 0.5 g of 3-aminopropyltriethoxysilane thereto, heating and reflux at 80° C. for 10-12 h, then performing solid-liquid separation to obtain a solid product, washing the solid with an ethanol-water mixture for 3-5 times, and vacuum drying to obtain silane-modified montmorillonite; the volume ratio of ethanol to deionized water in the ethanol-water mixture is 4:1;

[0144] b) Mixing silane-modified montmorillonite and humic acid in a mass ratio of 2:1, adding the mixture to 3 times the amount of deionized water, and ultrasonically dispersing the mixture for 10-15 minutes to obtain a suspension. Adjusting the pH of the suspension to 8.5, and reacting the mixture at 40-45°C and 200 r / min for 24 hours with magnetic stirring. After the reaction, freeze-drying the mixture to obtain the modified montmorillonite.

[0145] The composite microbial bacterial liquid is prepared by the following method: Bacillus coagulans and Bacillus cladocerus are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600=2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain two fermentation broths, and then mix them in a 1:1 volume ratio to obtain a composite microbial culture liquid.

[0146] The fermentation liquid medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, MgSO 4 0.05g, KH 2 PO 4 0.02g, distilled water 1000ml.

[0147] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0148] The preparation method of the above-mentioned high-efficiency domestic sewage treatment agent comprises the following steps:

[0149] S1: preparing composite microbial culture liquid;

[0150] S2: Preparation of modified montmorillonite;

[0151] S3: The modified montmorillonite and the composite microbial liquid are mixed in proportion, stirred and mixed evenly, cultured at 30° C. for 2-3 days, and the mixture is freeze-dried in vacuum to obtain a mixed powder;

[0152] S4: Evenly mix the mixed powder with the prepared composite material to obtain a high-efficiency domestic sewage treatment agent.

[0153] This comparative example is basically the same as Example 3, except that the composite microbial bacterial liquid does not contain Trichoderma viride.

[0154] Comparative Example 5

[0155] A high-efficiency domestic sewage treatment agent is made of the following raw materials in parts by weight: 70 kg of a composite material, 30 kg of modified montmorillonite, and 40 kg of a composite microbial liquid; the composite microbial liquid is made of Bacillus coagulans and Trichoderma viride; the composite material is an iron-based-biochar composite material; and the modified montmorillonite is humic acid-modified montmorillonite.

[0156] The composite material is prepared by the following method:

[0157] (1) Add 0.5 mol / L FeCl 3 Solution and 0.2 mol / L ZnCl 2 The solutions are mixed in equal proportions to obtain a mixed solution A;

[0158] (2) Disperse 2 g of modified biochar in 100 ml of deionized water and ultrasonically disperse for 30-40 min to obtain suspension B;

[0159] (3) Heat 150 ml of 3 mol / L NaOH solution to 75-85°C, then slowly add 50 ml of suspension B, add 50 ml of mixed solution A after the addition is complete, and continue to react with magnetic stirring for 3-4 hours. After the reaction is completed, wash the solid product to neutrality by solid-liquid separation, and vacuum dry to obtain the composite material.

[0160] The modified biochar is prepared by the following method: 100g of straw biochar is mixed with 2-3g of nano cuprous oxide, dispersed in 300ml of deionized water, ultrasonically dispersed for 10min, and then 75ml of 0.1mmol / L NaOH solution is added, magnetically stirred at a speed of 150r / min at room temperature for 10-12h, allowed to stand for 48h, filtered, and the solid is washed with deionized water to neutrality, and after drying, transferred to a muffle furnace and heated to 500°C at a rate of 5°C / min, and then calcined for 2h to obtain the modified biochar.

[0161] The modified montmorillonite is prepared by the following method:

[0162] a) adding 10 g of montmorillonite powder to 200 ml of an ethanol-water mixture, adding 0.5 g of 3-aminopropyltriethoxysilane thereto, heating and reflux at 80° C. for 10-12 h, then performing solid-liquid separation to obtain a solid product, washing the solid with an ethanol-water mixture for 3-5 times, and vacuum drying to obtain silane-modified montmorillonite; the volume ratio of ethanol to deionized water in the ethanol-water mixture is 4:1;

[0163] b) Mixing silane-modified montmorillonite and humic acid in a mass ratio of 2:1, adding the mixture to 3 times the amount of deionized water, and ultrasonically dispersing the mixture for 10-15 minutes to obtain a suspension. Adjusting the pH of the suspension to 8.5, and reacting the mixture at 40-45°C and 200 r / min for 24 hours with magnetic stirring. After the reaction, freeze-drying the mixture to obtain the modified montmorillonite.

[0164] The composite microbial bacterial liquid is prepared by the following method: Bacillus coagulans and Trichoderma viride are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600 =2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain two fermentation broths, and then mix them in a 1:1 volume ratio to obtain a composite microbial culture liquid.

[0165] The fermentation liquid medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, MgSO 4 0.05g, KH 2 PO 4 0.02g, distilled water 1000ml.

[0166] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0167] The preparation method of the above-mentioned high-efficiency domestic sewage treatment agent comprises the following steps:

[0168] S1: preparing composite microbial culture liquid;

[0169] S2: Preparation of modified montmorillonite;

[0170] S3: The modified montmorillonite and the composite microbial liquid are mixed in proportion, stirred and mixed evenly, cultured at 30° C. for 2-3 days, and the mixture is freeze-dried in vacuum to obtain a mixed powder;

[0171] S4: Evenly mix the mixed powder with the prepared composite material to obtain a high-efficiency domestic sewage treatment agent.

[0172] This comparative example is basically the same as Example 3, except that the composite microbial liquid does not contain Bacillus subtilis.

[0173] Comparative Example 6

[0174] A high-efficiency domestic sewage treatment agent is made of the following raw materials in parts by weight: 70 kg of a composite material, 30 kg of modified montmorillonite, and 40 kg of a composite microbial liquid; the composite microbial liquid is made of viride and Bacillus; the composite material is an iron-based-biochar composite material; and the modified montmorillonite is humic acid-modified montmorillonite.

[0175] The composite material is prepared by the following method:

[0176] (1) Add 0.5 mol / L FeCl 3 Solution and 0.2 mol / L ZnCl 2 The solutions are mixed in equal proportions to obtain a mixed solution A;

[0177] (2) Disperse 2 g of modified biochar in 100 ml of deionized water and ultrasonically disperse for 30-40 min to obtain suspension B;

[0178] (3) Heat 150 ml of 3 mol / L NaOH solution to 75-85°C, then slowly add 50 ml of suspension B, add 50 ml of mixed solution A after the addition is complete, and continue to react with magnetic stirring for 3-4 hours. After the reaction is completed, wash the solid product to neutrality by solid-liquid separation, and vacuum dry to obtain the composite material.

[0179] The modified biochar is prepared by the following method: 100g of straw biochar is mixed with 2-3g of nano cuprous oxide, dispersed in 300ml of deionized water, ultrasonically dispersed for 10min, and then 75ml of 0.1mmol / L NaOH solution is added, magnetically stirred at a speed of 150r / min at room temperature for 10-12h, allowed to stand for 48h, filtered, and the solid is washed with deionized water to neutrality, and after drying, transferred to a muffle furnace and heated to 500°C at a rate of 5°C / min, and then calcined for 2h to obtain the modified biochar.

[0180] The modified montmorillonite is prepared by the following method:

[0181] a) adding 10 g of montmorillonite powder to 200 ml of an ethanol-water mixture, adding 0.5 g of 3-aminopropyltriethoxysilane thereto, heating and reflux at 80° C. for 10-12 h, then performing solid-liquid separation to obtain a solid product, washing the solid with an ethanol-water mixture for 3-5 times, and vacuum drying to obtain silane-modified montmorillonite; the volume ratio of ethanol to deionized water in the ethanol-water mixture is 4:1;

[0182] b) Mixing silane-modified montmorillonite and humic acid in a mass ratio of 2:1, adding the mixture to 3 times the amount of deionized water, and ultrasonically dispersing the mixture for 10-15 minutes to obtain a suspension. Adjusting the pH of the suspension to 8.5, and reacting the mixture at 40-45°C and 200 r / min for 24 hours with magnetic stirring. After the reaction, freeze-drying the mixture to obtain the modified montmorillonite.

[0183] The composite microbial bacterial liquid is prepared by the following method: Trichoderma viride and Bacillus cladocerus are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600 =2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain two fermentation broths, and then mix them in a 1:1 volume ratio to obtain a composite microbial culture liquid.

[0184] The fermentation liquid medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, MgSO 4 0.05g, KH 2 PO4 0.02g, distilled water 1000ml.

[0185] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0186] The preparation method of the above-mentioned high-efficiency domestic sewage treatment agent comprises the following steps:

[0187] S1: preparing composite microbial culture liquid;

[0188] S2: Preparation of modified montmorillonite;

[0189] S3: The modified montmorillonite and the composite microbial liquid are mixed in proportion, stirred and mixed evenly, cultured at 30° C. for 2-3 days, and the mixture is freeze-dried in vacuum to obtain a mixed powder;

[0190] S4: Evenly mix the mixed powder with the prepared composite material to obtain a high-efficiency domestic sewage treatment agent.

[0191] This comparative example is basically the same as Example 3, the only difference being that the composite microbial bacterial liquid does not contain Bacillus coagulans.

[0192] Application Example 1

[0193] The same domestic sewage source was selected as the test water source, and 10 groups of domestic sewage of equal amount were taken therefrom and numbered 1-10 respectively, wherein 1-3 used the high-efficiency domestic sewage treatment agent of Examples 1-3 of the present invention, 4-9 used the domestic sewage treatment agent prepared in Comparative Examples 1-6, and Group 10 was a blank control group without using any sewage treatment agent. The water quality after treatment was measured, and the specific results are shown in Table 1.

[0194] Table 1 Water quality after treatment

[0195]

[0196] It can be seen from the contents of Table 1 that the contents of COD, BOD, ammonia nitrogen, total phosphorus and suspended solids in the sewage after using the treatment agents of Examples 1-3 of the present invention are greatly reduced, the COD removal rate is more than 98.4%, the BOD removal rate is more than 65.4%, and the ammonia nitrogen, total phosphorus and suspended solids indicators are also significantly lower than the discharge standards. At the same time, the treated sewage also has good chromaticity (such as Figure 1). It can be seen from the data of comparative examples 1-6 that the sewage treatment effects corresponding to the changes in the composite material composition, montmorillonite properties and microbial composition are all reduced. This is because the sewage treatment agent prepared by the present invention is a combination of physical adsorption-chemical degradation-biological treatment to deeply purify domestic sewage, and each raw material promotes each other and synergizes to enhance the efficiency, and none of them can be missing.

[0197] Application Example 2

[0198] In order to further illustrate the treatment effect of the sewage treatment agent of the present invention on sewage, the present invention also studies the treatment effect of different heavy metal ions in simulated sewage. 2+ 100mg / L, Zn 2+ 100mg / L, Pb 2+ 50mg / L, Ni 2+ 50 mg / L, and then treated with the high-efficiency domestic sewage treatment agents prepared in Examples 1-3 of the present invention and Comparative Examples 1-6, and the effects of different treatment groups on Cu at different concentrations were determined. 2+ 、Zn 2+ , Pb 2+ 、Ni 2+ The removal rate is shown in Table 2. Removal rate = (concentration before adding treatment agent - concentration after adding treatment agent) / concentration before adding treatment agent * 100%.

[0199] Table 2 Heavy metal ion content in treated water

[0200]

[0201] It can be seen from the contents of Table 2 above that the sewage treatment agent prepared by the present invention also has a good removal effect on heavy metal ions in water, and the corresponding effect of changing the composition of the sewage treatment agent raw materials tends to weaken.

[0202] It should be noted that the above embodiments are only partial embodiments of the preferred methods of implementing the present invention, rather than all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

Claims

1. A highly efficient domestic sewage treatment agent, characterized in that: The invention is made of the following raw materials in parts by weight: 60-80 parts of composite material, 20-40 parts of modified montmorillonite, and 30-50 parts of composite microbial liquid; the composite microbial liquid is made of Bacillus coagulans ( Bacillus )、Bacillus ( Virgibacillus )、Trichoderma viride( Trichoderma ); the Bacillus coagulans strain is numbered CCTCC CB 20082253, the preservation date is 2005-09-27, purchased from China Center for Type Culture Collection; the Bacillus cladodes strain is numbered CCTCC AA 207023, the preservation date is 2007-05-23, purchased from China Center for Type Culture Collection; the Trichoderma viride strain is numbered CCTCC CF 2008706, the preservation date is 2005-10-24, purchased from China Center for Type Culture Collection; the composite material is an iron-based-biochar composite material; the modified montmorillonite is humic acid modified montmorillonite; The composite material is prepared by the following method: (1) Mix 0.5 mol / L FeCl3 solution and 0.2 mol / L ZnCl2 solution in equal proportions to obtain a mixed solution A; (2) Disperse 2 g of modified biochar in 100 ml of deionized water and ultrasonically disperse for 30-40 min to obtain suspension B; (3) Heat a 3 mol / L NaOH solution to 75-85°C, then slowly add the suspension B thereto, add the mixed solution A thereto after the addition is complete, and continue to react with magnetic stirring for 3-4 hours. After the reaction is completed, wash the solid product to neutrality by solid-liquid separation, and vacuum dry to obtain a composite material; The modified montmorillonite is prepared by the following method: a) adding 10 g of montmorillonite powder to 200 ml of an ethanol-water mixture, adding 0.5 g of 3-aminopropyltriethoxysilane thereto, heating and reflux at 80° C. for 10-12 h, then performing solid-liquid separation to obtain a solid product, washing the solid with an ethanol-water mixture for 3-5 times, and vacuum drying to obtain silane-modified montmorillonite; the volume ratio of ethanol to deionized water in the ethanol-water mixture is 4:1; b) mixing silane-modified montmorillonite and humic acid in a mass ratio of 2:1, adding the mixture to 3 times the amount of deionized water, and ultrasonically dispersing the mixture for 10-15 minutes to obtain a suspension, adjusting the pH of the suspension to 8.5, and reacting the mixture at 40-45° C. and 200 r / min for 24 hours with magnetic stirring. After the reaction, freeze-drying the mixture to obtain modified montmorillonite; The modified biochar is prepared by the following method: straw biochar and nano cuprous oxide are mixed in a mass ratio of 100:2-3, dispersed in deionized water, ultrasonically dispersed for 10 minutes, and then an appropriate amount of NaOH solution is added. The mixture is magnetically stirred at a speed of 150 r / min at room temperature for 10-12 hours, allowed to stand for 48 hours, filtered, and the solid is washed with deionized water until it is neutral. After drying, the solid is transferred into a muffle furnace and calcined at high temperature for 2 hours to obtain the modified biochar.

2. The high-efficiency domestic sewage treating agent according to claim 1, characterized in that: The composite microbial bacterial liquid is prepared by the following method: Bacillus coagulans, Bacillus cladocerus, and Trichoderma viride are activated in PDA solid culture medium respectively, and then a single colony is picked and inoculated into a test tube of LB liquid culture medium, and cultured at 28°C and 150 r / min until OD 600 =2.0, inoculate 10% of the seed liquid into 200 mL of fermentation liquid medium, ferment and culture at 28°C and 180 r / min for 36 h, stop the fermentation to obtain three fermentation broths, and then mix them in a volume ratio of 1:1:1 to obtain a composite microbial culture liquid.

3. The high-efficiency domestic sewage treatment agent according to claim 1, characterized in that: In the step (3), the volume ratio of the NaOH solution, the mixed solution A and the suspension B is 3:1:

1.

4. The high-efficiency domestic sewage treating agent according to claim 1, characterized in that: The high temperature calcination is carried out by heating the temperature to 500°C at a rate of 5°C / min and then calcining for 2 hours; the straw biochar and nano cuprous oxide are mixed in a mass ratio of 100:2-3, dispersed in deionized water, ultrasonically dispersed for 10 minutes, and then an appropriate amount of NaOH solution is added, wherein the concentration of the NaOH solution is 0.1mmol / L, the volume ratio of the NaOH solution to the deionized water is 1:4, and the mass of the deionized water is 3 times the mass of the straw biochar.

5. The high-efficiency domestic sewage treating agent according to claim 2, characterized in that: The fermentation liquid culture medium is composed of: 3 g yeast extract, 5 g glucose, 15 g molasses, 3 g peptone, 0.05 g MgSO4, 0.02 g KH2PO4, and 1000 ml distilled water.

6. A method for preparing a high-efficiency domestic sewage treatment agent according to any one of claims 1 to 5, characterized in that: It includes the following steps: S1: preparing composite microbial culture liquid; S2: Preparation of modified montmorillonite; S3: The modified montmorillonite and the composite microbial liquid are mixed in proportion, stirred and mixed evenly, cultured at 30° C. for 2-3 days, and the mixture is freeze-dried in vacuum to obtain a mixed powder; S4: Evenly mix the mixed powder with the prepared composite material to obtain a high-efficiency domestic sewage treatment agent.

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