Method for reducing 3-chloropropanol ester and glycidyl ester in grease

Abstract

The invention discloses a method for reducing 3-chloropropanol ester and glycidyl ester in grease, and relates to the technical field of grease. The invention relates to a method for reducing 3-chloropropanol ester and glycidyl ester in grease, which comprises the following steps: centrifugal separation: taking a grease material, adding lipase and purified water while stirring in a nitrogen environment, carrying out a stirring reaction, then carrying out centrifugal layering, and taking an upper-layer oil material; activated carbon treatment: adding activated carbon into the obtained oil material, stirring and reacting in a nitrogen environment, and then filtering to obtain a product. According to the method, 3-chloropropanol ester and glycidyl ester in grease can be effectively reduced, meanwhile, the process reaction condition is mild, the process operation is simple and convenient, and the production process cost can be effectively controlled.

Description

Technical Field

[0001] The present application relates to the technical field of oils and fats, and in particular to a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats. Background Art

[0002] Chloropropanol esters are the esterification products of chloropropanol compounds and fatty acids. According to the different types of chloropropanol, they are divided into 3-chloropropanol esters (3-MCPD esters), 2-chloro-1,3-propanediol esters (2-MCPD esters), 1,3-dichloro-2-propanol esters (1,3-DCP esters) and 2,3-dichloro-2-propanol esters (2,3-DCP esters). 3-Chloropropanol esters are detected in higher amounts in food oils and fats.

[0003] Glycidyl ester is the esterification product of fatty acid and glycidol. It is a twin brother of 3-chloropropanol ester and has a similar formation mechanism. In the oil refining process, glycidyl ester is usually formed together with 3-chloropropanol ester. The higher the content of 3-chloropropanol ester, the higher the content of glycidyl ester.

[0004] During the high-temperature (≥200℃) refining process of edible oils and fats, 3-chloropropanol esters and glycidyl esters are formed. Toxicological studies have shown that 3-chloropropanol esters have kidney, reproductive, and non-genotoxic effects, while glycidyl esters are carcinogens with genetic toxicity. Therefore, it is necessary to reduce the content of 3-chloropropanol esters and glycidyl esters in edible oil and fat products. Summary of the invention

[0005] The purpose of the present application is to provide a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which can effectively reduce 3-chloropropanol esters and glycidyl esters in oils and fats, and at the same time, the process reaction conditions are mild, the process operation is simple and convenient, and the production process cost can be effectively controlled.

[0006] The technical solution of this application is as follows:

[0007] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0008] Centrifugal separation: Take the oil material, add lipase and purified water under nitrogen environment while stirring, stir and react, then centrifuge to separate the layers and take the upper layer of oil;

[0009] Activated carbon treatment: Add activated carbon to the obtained oil, stir and react under a nitrogen environment, and then filter to obtain the product.

[0010] Furthermore, in some embodiments of the present application, in the above-mentioned centrifugal separation step, the above-mentioned oil material is ethyl ester type fish oil or triglyceride type fish oil, which contains 3-chloropropanol ester and glycidyl ester.

[0011] Furthermore, in some embodiments of the present application, in the centrifugation step, the lipase is any one of Candida antarctica lipase B, Pseudomonas lipase, and Candida columnaris lipase.

[0012] Furthermore, in some embodiments of the present application, in the above-mentioned centrifugal separation step, lipase and purified water are added to the oil material while stirring at a temperature of 35-50°C in a nitrogen environment, and the stirring reaction is continued for 2 hours, and then centrifuged to separate the layers and take the upper layer of oil; wherein the stirring rate is 100-300r / min.

[0013] Furthermore, in some embodiments of the present application, in the above-mentioned centrifugal separation step, after centrifugal separation and taking the upper layer of oil, purified water is added to the oil for washing, and the washing is repeated twice, and then the lower layer of water is removed by separation.

[0014] Furthermore, in some embodiments of the present application, when purified water is added to the oil for washing, the mass ratio of the added purified water to the oil is 1:1; and each washing time is 10 minutes.

[0015] Furthermore, in some embodiments of the present application, in the above-mentioned centrifugal separation step, when lipase and purified water are added for stirring reaction, the amount of lipase added is 0.05-0.2% of the mass of the oil material, and the mass ratio of the added purified water to the oil material is (0.5-2):1.

[0016] Furthermore, in some embodiments of the present application, in the above-mentioned activated carbon treatment step, the mass of activated carbon added is 1% of the mass of the oil; after adding the activated carbon, the reaction is stirred for 4 hours in a nitrogen environment.

[0017] Further, in some embodiments of the present application, in the centrifugation step, the lipase is any one of a lipase derived from Trichoderma reesei, a lipase derived from porcine pancreas (PPL), Candida plicata lipase, Aspergillus oryzae lipase, Aspergillus niger lipase, lipase RM IM, or lipase TL IM.

[0018] Compared with the prior art, the embodiments of the present application have at least the following advantages or beneficial effects:

[0019] In view of the above aspects, the embodiments of the present application provide a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which utilizes lipase and activated carbon to react with oil materials, thereby effectively reducing 3-chloropropanol esters and glycidyl esters contained in the oil materials; compared with other traditional chemical methods, the reaction conditions are milder and the process temperature is lower. At the same time, the material input amount in the process method is small, which can effectively reduce the energy consumption cost of the production process and the raw material cost, thereby improving the production economic benefits and being more conducive to actual production applications. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to the conventional conditions or the conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0021] It should be noted that the term "includes" or any other variation thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "includes..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0022] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application may be combined with each other.

[0023] The features and performance of the present application are further described in detail below in conjunction with the embodiments.

[0024] Example 1

[0025] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0026] Centrifugal separation: Take 1000g of ethyl ester fish oil (or triglyceride fish oil), add Antarctic Candida lipase B and 1000g of purified water under nitrogen environment at a stirring rate of 300r / min and 40°C while stirring, continue stirring and reacting for 2h, then centrifuge and separate the layers, take the upper layer of oil, add purified water and stir and wash for 10min, repeat washing twice, and then separate the layers to remove the lower layer of water;

[0027] The amount of Antarctic Candida lipase B added is 0.05% of the mass of ethyl ester fish oil (or triglyceride fish oil), and the mass ratio of purified water to ethyl ester fish oil (or triglyceride fish oil) added during washing is 1:1;

[0028] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0029] The mass of the activated carbon added is 1% of the mass of the ethyl ester fish oil (or triglyceride fish oil).

[0030] In this example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0031] Example 2

[0032] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0033] Centrifugal separation: Take 1000g of ethyl ester fish oil (or triglyceride fish oil), add Pseudomonas lipase and 1000g of purified water under nitrogen environment at a stirring rate of 300r / min and 40°C while stirring, continue stirring and react for 2h, then centrifuge and separate the layers, take the upper layer of oil, add purified water and stir and wash for 10min, repeat washing twice, then separate the layers and remove the lower layer of water;

[0034] The amount of Pseudomonas lipase added is 0.05% of the mass of ethyl ester fish oil (or triglyceride fish oil), and the mass ratio of purified water to ethyl ester fish oil (or triglyceride fish oil) added during washing is 1:1;

[0035] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0036] The mass of the activated carbon added is 1% of the mass of the ethyl ester fish oil (or triglyceride fish oil).

[0037] In this example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0038] Example 3

[0039] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0040] Centrifugal separation: Take 1000g of ethyl ester fish oil (or triglyceride fish oil), add columnar Candida lipase and 1000g of purified water under nitrogen environment at a stirring rate of 300r / min and 40°C while stirring, continue stirring and react for 2h, then centrifuge and separate the layers, take the upper layer of oil, add purified water and stir and wash for 10min, repeat washing twice, then separate the layers and remove the lower layer of water;

[0041] The amount of the added Candida columnaris lipase is 0.05% of the mass of the ethyl ester fish oil (or triglyceride fish oil), and the mass ratio of the added purified water to the ethyl ester fish oil (or triglyceride fish oil) during washing is 1:1;

[0042] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0043] The mass of the activated carbon added is 1% of the mass of the ethyl ester fish oil (or triglyceride fish oil).

[0044] In this example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0045] Example 4

[0046] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0047] Centrifugal separation: Take 1000g of ethyl ester fish oil (or triglyceride fish oil), add columnar Candida lipase and 1000g of purified water under nitrogen environment at a stirring rate of 300r / min and 35°C while stirring, continue stirring and react for 2h, then centrifuge and separate the layers, take the upper layer of oil, add purified water and stir and wash for 10min, repeat washing twice, then separate the layers and remove the lower layer of water;

[0048] The amount of the added Candida columnaris lipase is 0.05% of the mass of the ethyl ester fish oil (or triglyceride fish oil), and the mass ratio of the added purified water to the ethyl ester fish oil (or triglyceride fish oil) during washing is 1:1;

[0049] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0050] The mass of the activated carbon added is 1% of the mass of the ethyl ester fish oil (or triglyceride fish oil).

[0051] In this example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0052] Example 5

[0053] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0054] Centrifugal separation: Take 1000g of ethyl ester fish oil (or triglyceride fish oil), add columnar Candida lipase and 1000g of purified water under nitrogen environment at a stirring rate of 300r / min and 50°C while stirring, continue stirring and react for 2h, then centrifuge and separate the layers, take the upper layer of oil, add purified water and stir and wash for 10min, repeat washing twice, then separate the layers and remove the lower layer of water;

[0055] The amount of the added Candida columnaris lipase is 0.05% of the mass of the ethyl ester fish oil (or triglyceride fish oil), and the mass ratio of the added purified water to the ethyl ester fish oil (or triglyceride fish oil) during washing is 1:1;

[0056] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0057] The mass of the activated carbon added is 1% of the mass of the ethyl ester fish oil (or triglyceride fish oil).

[0058] In this example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0059] Example 6

[0060] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0061] Centrifugal separation: Take 1200 kg of ethyl ester fish oil (or triglyceride fish oil), add columnar Candida lipase and 1200 kg of purified water under nitrogen environment at a stirring rate of 300 r / min and 40°C while stirring, continue stirring and reacting for 2 hours, then centrifuge and separate the layers, take the upper layer of oil, add purified water and stir and wash for 10 minutes, repeat washing twice, and then separate the layers to remove the lower layer of water;

[0062] The amount of the added Candida columnaris lipase is 0.05% of the mass of the ethyl ester fish oil (or triglyceride fish oil), and the mass ratio of the added purified water to the ethyl ester fish oil (or triglyceride fish oil) during washing is 1:1;

[0063] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0064] The mass of the activated carbon added is 1% of the mass of the ethyl ester fish oil (or triglyceride fish oil).

[0065] In this example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0066] Example 7

[0067] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0068] Centrifugal separation: Take 1000g of ethyl ester fish oil (or triglyceride fish oil), add columnar Candida lipase and 500g of purified water under nitrogen environment at a stirring rate of 300r / min and 50°C while stirring, continue stirring and react for 2h, then centrifuge and separate the layers, take the upper layer of oil, add purified water and stir and wash for 10min, repeat washing twice, then separate the layers and remove the lower layer of water;

[0069] The amount of the added Candida columnaris lipase is 0.05% of the mass of the ethyl ester fish oil (or triglyceride fish oil), and the mass ratio of the added purified water to the ethyl ester fish oil (or triglyceride fish oil) during washing is 1:1;

[0070] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0071] The mass of the activated carbon added is 1% of the mass of the ethyl ester fish oil (or triglyceride fish oil).

[0072] In this example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0073] Example 8

[0074] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0075] Centrifugal separation: Take 1000g of ethyl ester fish oil (or triglyceride fish oil), add columnar Candida lipase and 1500g of purified water under nitrogen environment at a stirring rate of 300r / min and 50°C while stirring, continue stirring and react for 2h, then centrifuge and separate the layers, take the upper layer of oil, add purified water and stir and wash for 10min, repeat washing twice, then separate the layers and remove the lower layer of water;

[0076] The amount of the added Candida columnaris lipase is 0.05% of the mass of the ethyl ester fish oil (or triglyceride fish oil), and the mass ratio of the added purified water to the ethyl ester fish oil (or triglyceride fish oil) during washing is 1:1;

[0077] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0078] The mass of the activated carbon added is 1% of the mass of the ethyl ester fish oil (or triglyceride fish oil).

[0079] In this example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0080] Example 9

[0081] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0082] Centrifugal separation: Take 1000g of ethyl ester fish oil (or triglyceride fish oil), add columnar Candida lipase and 1000g of purified water under nitrogen environment at a stirring rate of 300r / min and 50°C while stirring, continue stirring and react for 2h, then centrifuge and separate the layers, take the upper layer of oil, add purified water and stir and wash for 10min, repeat washing twice, then separate the layers and remove the lower layer of water;

[0083] The amount of the added Candida columnaris lipase is 0.2% of the mass of the ethyl ester fish oil (or triglyceride fish oil), and the mass ratio of the added purified water to the ethyl ester fish oil (or triglyceride fish oil) during washing is 1:1;

[0084] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0085] The mass of the activated carbon added is 1% of the mass of the ethyl ester fish oil (or triglyceride fish oil).

[0086] In this example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0087] Example 10

[0088] The present invention provides a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which comprises the following steps:

[0089] Centrifugal separation: Take 1000g of ethyl ester fish oil (or triglyceride fish oil), add columnar Candida lipase and 1000g of purified water under nitrogen environment at a stirring rate of 150r / min and 50°C while stirring, continue stirring and react for 2h, then centrifuge and separate the layers, take the upper layer of oil, add purified water and stir and wash for 10min, repeat washing twice, then separate the layers and remove the lower layer of water;

[0090] The amount of the added Candida columnaris lipase is 0.05% of the mass of the ethyl ester fish oil (or triglyceride fish oil), and the mass ratio of the added purified water to the ethyl ester fish oil (or triglyceride fish oil) during washing is 1:1;

[0091] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0092] The mass of the activated carbon added is 1% of the mass of the ethyl ester fish oil (or triglyceride fish oil).

[0093] In this example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0094] Comparative Example 1

[0095] This comparative example uses a chemical method to treat ethyl ester fish oil, and the specific steps are:

[0096] Take 1000g of ethyl ester fish oil, add 1g of 40% sodium hydroxide solution and 20% anhydrous ethanol by weight of ethyl ester fish oil under nitrogen environment at a stirring rate of 300r / min and 80°C while stirring, continue stirring and reacting for 1h, then centrifuge and separate, take the upper layer of oil, add purified water and stir and wash for 10min, repeat washing twice, and then separate and remove the lower layer of water;

[0097] The mass ratio of purified water to ethyl ester fish oil added during washing is 1:1;

[0098] Activated carbon treatment: Add activated carbon to the washed oil, stir and react for 4 hours under nitrogen environment, then filter to obtain the product;

[0099] The mass of activated carbon added is 1% of the mass of ethyl ester fish oil.

[0100] In this comparative example, the ethyl ester fish oil (or triglyceride fish oil) used contains 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0101] Comparative Example 2

[0102] In this comparative example, the oil material is directly treated with activated carbon, and the specific steps are as follows:

[0103] Take 1000g of ethyl ester fish oil, add activated carbon with a mass percentage of 1% of the ethyl ester fish oil under nitrogen environment, stir and react at 80°C for 4h, and then filter to obtain the product;

[0104] In this comparative example, the ethyl ester fish oil used contained 5.95 mg / kg of 3-chloropropanol ester and 1.34 mg / kg of glycidyl ester.

[0105] Test example

[0106] The products obtained in Examples 1 to 6 and Comparative Examples 1 to 2 were tested, and the testing method was as follows:

[0107] Take 50g / bottle of the product, take two bottles in total, and test the content of 3-chloropropanol ester and glycidyl ester in the product according to ISO 18363-4:2021 method. The results are shown in Table 1:

[0108] Table 1

[0109]

[0110] It can be seen from the results that in Examples 1 to 3, the use of Antarctic Candida lipase B, Pseudomonas lipase and Candida columnaris lipase, respectively, can effectively reduce the content of 3-chloropropanol ester and glycidyl ester in ethyl ester fish oil (or triglyceride fish oil), and the effect is obvious;

[0111] In Examples 4 to 5, based on Example 3, the reaction temperature after adding lipase was changed (35° C., 50° C.), which can also effectively reduce the content of 3-chloropropanol ester and glycidyl ester in ethyl ester fish oil (or triglyceride fish oil);

[0112] Example 6 is scaled up on the basis of Example 3, and the raw material input level (1200 kg) is increased. The solution can still effectively reduce the content of 3-chloropropanol ester and glycidyl ester in ethyl ester fish oil (or triglyceride fish oil), indicating that it can be effectively applied to industrial-scale production;

[0113] In Examples 7 to 8, based on Example 5, the amount of purified water added was changed to 500 g (the mass ratio to the oil material was 0.5:1) and 1500 g (the mass ratio to the oil material was 1.5:1), respectively. The schemes can also effectively reduce the content of 3-chloropropanol ester and glycidyl ester in ethyl ester fish oil (or triglyceride fish oil);

[0114] Example 9 is based on Example 5, in which the amount of lipase added during the stirring reaction is changed to 0.2% of the oil material. This solution can also effectively reduce the content of 3-chloropropanol ester and glycidyl ester in ethyl ester fish oil (or triglyceride fish oil); relatively speaking, the amount of lipase used in Example 9 is high, the cost is higher, but the effect is better; the amount of lipase used in Example 5 is less, and the cost is lower.

[0115] Example 10 changes the stirring rate during the stirring reaction to 150 r / min based on Example 5. This solution can also effectively reduce the content of 3-chloropropanol ester and glycidyl ester in ethyl ester fish oil (or triglyceride fish oil).

[0116] Comparative Example 1 uses sodium hydroxide and anhydrous ethanol for chemical treatment. Although it can also reduce the content of 3-chloropropanol ester and glycidyl ester, its process temperature condition requirement is relatively high (80° C.) and the energy consumption is large;

[0117] Comparative Example 2 was not treated with lipase but directly treated with activated carbon. The results showed that the effect of this scheme was extremely poor and it was difficult to reduce the content of 3-chloropropanol ester and glycidyl ester in ethyl ester fish oil (or triglyceride fish oil).

[0118] In summary, the embodiments of the present application provide a method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, which utilizes lipase and activated carbon to react with oil materials, and can effectively reduce 3-chloropropanol esters and glycidyl esters contained in oil materials; compared with other traditional chemical methods, the reaction conditions are milder and the process temperature is lower. At the same time, the material input amount in the process method is small, which can effectively reduce the energy consumption cost of the production process and the raw material cost, thereby improving the production economic benefits, and is more conducive to actual production applications.

[0119] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

Claims

1. A method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats, characterized in that: It includes the following steps: Centrifugal separation: Take the oil material, add lipase and purified water under nitrogen environment while stirring, stir and react, then centrifuge to separate the layers and take the upper layer of oil; Activated carbon treatment: Add activated carbon to the obtained oil, stir and react under a nitrogen environment, and then filter to obtain the product.

2. The method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats according to claim 1, characterized in that: In the centrifugal separation step, the oil material is ethyl ester type fish oil or triglyceride type fish oil, which contains 3-chloropropanol ester and glycidyl ester.

3. The method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats according to claim 1, characterized in that: In the centrifugal separation step, the lipase is any one of Candida antarctica lipase B, Pseudomonas lipase, and Candida columnaris lipase.

4. The method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats according to claim 1, characterized in that: In the centrifugal separation step, lipase and purified water are added to the oil material under nitrogen environment at a temperature of 35-50°C while stirring, and the stirring reaction is continued for 2 hours, and then centrifuged to separate the layers and take the upper layer of oil; wherein the stirring rate is 100-300r / min.

5. The method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats according to claim 1, characterized in that: In the centrifugal separation step, after centrifugation and separation and taking the upper layer of oil, purified water is added to the oil for washing, and the washing is repeated twice, and then the lower layer of water is removed by separation.

6. The method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats according to claim 5, characterized in that: When purified water is added to the oil for washing, the mass ratio of the added purified water to the oil is 1:1; each washing time is 10 minutes.

7. The method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats according to claim 1, characterized in that: In the centrifugal separation step, when lipase and purified water are added for stirring reaction, the amount of lipase added is 0.05-0.2% of the mass of the oil material, and the mass ratio of purified water added to the oil material is (0.5-2):

1.

8. The method for reducing 3-chloropropanol esters and glycidyl esters in oils and fats according to claim 1, characterized in that: In the activated carbon treatment step, the mass of activated carbon added is 1% of the mass of the oil; after adding the activated carbon, the reaction is stirred for 4 hours under a nitrogen environment.

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