Device and method for collecting DNA (deoxyribonucleic acid) in sandy water environment

Abstract

The invention relates to the technical field of water body information collection, in particular to a sandy water body environment DNA collection device and method, and the device comprises a collection mechanism which comprises collection boxes arranged in a vertical direction in an array manner, a suspension plate arranged above the collection boxes, and a traction rope used for connecting the collection boxes or connecting the collection boxes and the suspension plate, the collection box comprises an outer protection box and a box cover fixedly connected to the front end of the outer protection box through bolts. The sand cleaning mechanism comprises a filter plate and a sand cleaning assembly, the filter plate is provided with a plurality of V-shaped plates which are connected in parallel in the vertical direction, a plurality of filter holes are formed in the V-shaped plates in an array mode, a first outer protective frame which extends backwards and has an L-shaped vertical section is fixedly arranged on the outer side of the filter plate, and a filter window is formed in the box cover; according to the collection device, DNA collection in a sandy water body can be achieved, the situation that the collection efficiency is reduced due to sand and stone blockage is effectively avoided, and the overall practicability is good.

Description

Technical Field

[0001] The present invention relates to the technical field of water body information collection, and in particular to a DNA collection device and a collection method for a sandy water environment. Background Art

[0002] Species distribution and species richness are the main issues in the study of species diversity in aquatic ecosystems. These play a vital role in the implementation of biodiversity protection measures. Obtaining DNA from the species' living environment to reflect species distribution, abundance and other information is a non-invasive and rapid means of species detection. In the process of collecting DNA information in the water environment, the staff will place the collection device in the target water area. Through the circulation of water and the setting of the collection bag, the DNA information in the water body can be collected. The collected DNA information will be collected by the staff and then studied and analyzed, so as to understand and grasp the biodiversity of the target water area. For example, the existing public document CN109406215B-A water environment DNA intelligent collection device and collection method and the existing public document CN219470046U-A water environment DNA sample collection device both disclose a water environment DNA sample collection device. Although the above collection device can realize the effective collection of DNA information in the water body, the existing collection device still has the following deficiencies in actual use: 1. The existing water environment DNA collection device is not suitable for environmental DNA collection in sandy environments. Because of the existence of sandy environments, after a period of use, a certain amount of sand and stones will accumulate at the collection port of the existing water environment DNA collection device. The long-term sand and stone piles are likely to block the collection port, resulting in the existing environmental DNA collection device being unable to be used normally; 2. The existing water environmental DNA collection device is in a fixed area of ​​the water body when in use. However, in waters with large depths, the environmental DNA information of waters with different depths is different. If the existing collection device is used for environmental DNA collection, the collected DNA information will be insufficiently rich, and the information obtained cannot fully reflect the distribution and abundance of species; Therefore, it is necessary to improve the prior art to solve the above-mentioned technical problems. Summary of the invention

[0003] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0004] In view of the problem that the above-mentioned existing water environment DNA collection device is not suitable for the collection of DNA information in sandy water environment, a sandy water environment DNA collection device is proposed.

[0005] To solve the above technical problems, the present invention provides the following technical solutions: a device for collecting DNA in a sandy water environment, comprising: a collecting mechanism, including collecting boxes arranged in an array along a vertical direction, a suspension board arranged above the collecting boxes, and a traction rope used to connect the collecting boxes or between the collecting boxes and the suspension board, the collecting box including an outer protective box and a box cover fixedly connected to the front end of the outer protective box by bolts; and a sand cleaning mechanism, including a filter plate and a sand cleaning assembly, the filter plate has a plurality of V-shaped plates arranged in parallel along a vertical direction, and a plurality of filter holes are arranged in an array on the V-shaped plate, a first outer protective frame extending backward and having an L-shaped vertical cross-section is fixedly provided on the outer side of the filter plate, a filter window is provided on the box cover, and the first outer protective frame is fixedly connected to the filter window by bolts, and the sand cleaning assembly includes a screw rod, a first conical gear Wheels and triangular blocks, each V-plate is fitted with a triangular block on its inner concave surface, a connecting head is fixedly provided in the middle of one side of the triangular block, a plurality of connecting heads are fixedly connected by a linkage rod, the upper end of the linkage rod is fixedly connected to one end of a driving plate by a connecting rod, the driving plate is spirally fitted on a screw rod, the screw rod is arranged above the outer protective box, and both ends of the screw rod are rotatably sleeved on a support plate through rolling bearings, the support plate is fixedly connected to the top surface of the outer protective box by bolts, and third bevel gears are symmetrically fixedly provided at both ends of the screw rod, one of the third bevel gears is meshed with the first bevel gear below, a second rotating column is fixedly provided on the bottom surface of the first bevel gear, and at the same time, the other end of the second rotating column slides through the top plate of the outer protective box and is embedded in the output end of the motor, and the motor is fixedly provided on the inner top surface of the outer protective box.

[0006] The beneficial effects of the present invention are as follows: when the environmental DNA collection device is in use, after being used for a period of time, the rotation of the first bevel gear is achieved by controlling the operation of the motor. Because the first bevel gear is meshed with the third bevel gear, the rotation of the screw rod is achieved. Because of the spiral matching between the screw rod and the drive plate, the drive plate can be moved along the axis of the screw rod under the rotation of the screw rod, and finally the linkage rod drives the synchronous movement of multiple triangular blocks. The triangular blocks are fitted on the concave surface of the V-plate, and the triangular blocks can push the excess sand and gravel accumulated on the filter plate from one side of the filter plate, thereby realizing the cleaning of the sand and gravel on the surface of the filter plate, and ensuring the efficient collection of water environmental DNA.

[0007] As a preferred solution of the DNA collection device for a sandy water environment of the present invention, V-shaped scrapers are symmetrically fixed on both side surfaces of the triangular block, and the scrapers are fitted with the inner concave surface of the V-plate, and the cross-section of the scrapers is triangular.

[0008] As a preferred solution of the DNA collection device for a sandy water environment of the present invention, a sealing ring is fixedly sleeved on the side wall of the second rotating column, and an oil seal groove is circumferentially provided on the outer wall of the sealing ring, and a second sealing groove for gap fitting of the sealing ring is provided on the top plate of the outer protective box; a guide block is fixedly provided on the other end of the driving plate to which the linkage rod is fixed, and a guide groove for gap fitting of the guide block is provided on the top surface of the outer protective box along the axial direction of the screw rod.

[0009] As a preferred solution of the DNA collection device for a sandy water environment of the present invention, a U-shaped frame with an upper end opening is fixedly provided on one side of the box cover close to the outer protective box, a slot for sliding and inserting a matching frame is opened on the inner side wall of the U-shaped frame, and a collection bag is fixedly provided on the inner side wall of the matching frame; a water outlet pipe is fixedly provided on the rear side of the outer protective box, and a water pump is fixedly installed on the water outlet pipe.

[0010] As a preferred solution of the DNA collection device for a sandy water environment of the present invention, a first sealing groove is provided on the front side of the outer protective box, and a sealing frame for gap fitting in the first sealing groove is fixedly provided on one side surface of the box cover; a waterproof cover is fixedly connected to the inner top surface of the outer protective box by bolts.

[0011] As a preferred solution of the DNA collection device for a sandy water environment of the present invention, the sand cleaning component also includes a water fan blade and an installation box, a first rotating column is fixedly arranged on the bottom surface of the water fan blade, the lower end of the first rotating column slides through the top plate of the outer protective box and is fixedly connected to the I-shaped disk, and a second bevel gear that meshes with the third bevel gear is symmetrically fixed on the first rotating column above and below the third bevel gear; a linkage plate is slidably sleeved at the groove position of the I-shaped disk, and T-shaped columns are symmetrically sleeved at both ends of the linkage plate, and the T-shaped A spring is slidably sleeved on the column, a magnetic plate is fixedly arranged on the bottom surface of the linkage plate between the I-shaped disk and the T-shaped column, the mounting box cover is closed on the outside of the linkage plate, and the mounting box is fixedly connected to the inner top surface of the outer protective box by bolts, an electromagnetic plate is fixedly arranged on the bottom surface of the mounting box, and the side surface close to the electromagnetic plate and the magnetic plate are arranged in the same sex; a detection groove is opened on the side wall of the first rotating column on the inner side of the mounting box along the circumferential direction, and the diameter of the detection groove is gradually increased or decreased from one end to the other end, and a distance measuring sensor is fixedly sleeved on the side wall of the mounting box on one side of the detection groove.

[0012] In view of the problem that the existing collection device collects information in fixed waters and the richness of the collected data is low, the present invention makes further optimization and improvement to a DNA collection device for a sandy water environment, wherein: it also includes an adjustment mechanism, the adjustment mechanism includes an adjustment airbag and a first conveying pipe, a U-shaped groove is provided at the lower end of the outer protective box on the rear side of the sand cleaning mechanism, the adjustment airbag is U-shaped and is arranged in the U-shaped groove, second outer protective frames are symmetrically fixed on both sides of the adjustment airbag, and the two upper ends of the two second outer protective frames are respectively fixedly connected by extension plates, a connecting strip is fixedly provided on the outer side wall of the outer protective box at the upper end of the U-shaped groove, and the connection between the connecting strip and the extension plate The two adjusting air bags are fixedly connected by bolts; three-way pipes are symmetrically fixed on the upper ends of both sides of the adjusting air bag, and a second solenoid valve is fixedly installed on the tube body connected to the adjusting air bag in the three-way pipe, and the three-way pipes on the two adjacent adjusting air bags that are aligned up and down are fixedly connected by a first delivery pipe; a straight pipe is symmetrically fixedly connected to the suspension board, and the straight pipe and the three-way pipe are arranged in a one-to-one correspondence, the three-way pipe on the topmost adjusting air bag is connected to the corresponding straight pipe through the first delivery pipe, a first solenoid valve is fixedly installed on the straight pipe, the upper end of one of the straight pipes is connected to the output end of the air pump through the second delivery pipe, and the air pump is fixedly installed on the top surface of the suspension board.

[0013] Another beneficial effect of the present invention is that when the environmental DNA collection device is in use, air can be blown out from the output end through the operation of the air pump. Through the transportation of the first air pipe and the design of the second solenoid valve on the three-way pipe, the amount of gas in at least one regulating air bag can be adjusted in a targeted manner. According to the principle of buoyancy, when the amount of gas in the regulating air bag changes, the buoyancy of the collection box in the water will change. In this way, the height of the collection box in the water body can be adjusted, so that environmental DNA at different depths of the water body can be collected, thereby improving the richness of the collected information and having good applicability.

[0014] As a preferred solution of the DNA collection device for a sandy water environment of the present invention, a plug-in column is fixedly provided at the corner position of the second outer protective frame, and a socket for the sliding insertion of the plug-in column is opened on the outer protective box.

[0015] As a preferred solution of the DNA collection device for a sandy water environment of the present invention, wherein: internally threaded through sleeves are symmetrically fixed on the two symmetrical side walls of the outer protective box, an internally threaded blind sleeve is fixed on the bottom surface of the suspension plate, and traction ropes are arranged between the two upper and lower adjacent internally threaded through sleeves and between the internally threaded through sleeve and the internally threaded blind sleeve, and threaded columns are symmetrically fixed on both ends of the traction rope, one of the threaded columns is spirally fitted on the internally threaded through sleeve, and the other threaded column is spirally sleeved on the internally threaded through sleeve or the internally threaded blind sleeve; a suspension air bag is fixed on the bottom surface of the suspension plate, and mounting holes are opened at the four corners of the suspension plate.

[0016] In addition, the present invention also provides the following technical solutions: a method for collecting DNA from a sandy water environment, wherein the above-mentioned sandy water environment DNA collection device performs a collection operation according to the following steps; S1: Select a corresponding number of collection boxes according to the water depth and the richness of the collected samples, and fix the multiple collection boxes and the collection boxes and the suspension board with traction ropes; S2: Lower the collection mechanism to the designated area manually or with a lifting device; S3: The collection mechanism collects the water environment DNA. When the collection reaches the preset time, the sand removal component is controlled to work to remove the sand from the outside of the filter plate, and then the sand removal is repeated at a preset time period; S4: During the collection process, the air pump, the first solenoid valve and the second solenoid valve are controlled to work together to inflate and deflate the regulating airbag, thereby adjusting the position of the collection box in the vertical direction; S5: After the collection is completed, the collection mechanism is salvaged by manual or lifting equipment, and the staff can collect and store the samples in the collection bag. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them: Figure 1 The figure is a schematic diagram of the overall structure of a DNA collection device for a sandy water environment in the present invention.

[0018] Figure 2 For the present invention Figure 1 Left rear view of the structure.

[0019] Figure 3 It is a schematic diagram of the coordination of the collection box, the sand cleaning mechanism and the regulating airbag in the present invention.

[0020] Figure 4 For the present invention Figure 3 Left rear view of the structure.

[0021] Figure 5 For the present invention Figure 3 Vertical cross-section of the structure in the front-to-back direction.

[0022] Figure 6 For the present invention Figure 3 Vertical cross-sectional view of the structure along the axis of the screw rod.

[0023] Figure 7 For the present invention Figure 3 Exploded view of the structure.

[0024] Figure 8 For the present invention Figure 7 Left rear view of the structure.

[0025] Fig. 9 It is an exploded view of the sand cleaning component in the present invention.

[0026] Fig.10 It is a schematic diagram of the bottom structure of the outer protective box in the present invention.

[0027] Fig.11 It is a schematic diagram of the overall structure of the regulating airbag in the present invention.

[0028] Fig.12 It is a schematic diagram of the bottom structure of the suspension board in the present invention. DETAILED DESCRIPTION

[0029] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.

[0030] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0031] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0032] Secondly, the present invention is described in detail with reference to the schematic diagram. When describing the embodiments of the present invention in detail, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included. Example 1

[0033] Reference Figure 1 , Figure 2 , Figure 3 and Figure 4, which is the first embodiment of the present invention, provides a DNA collection device for a sandy water environment. When the environmental DNA collection device is in use, multiple collection mechanisms 100 are suitable for collecting DNA information at different depths of the water body, and the sand clearing mechanism 200 can regularly clean the sand and stones during collection.

[0034] Specifically, it includes a collection mechanism 100, including collection boxes 101 arranged in an array along the vertical direction, a suspension board 102 arranged above the collection boxes 101 and a traction rope 103 used to connect the collection boxes 101 or between the collection boxes 101 and the suspension board 102, the collection box 101 includes an outer protective box 101a and a box cover 101b fixedly connected to the front end of the outer protective box 101a by bolts; and a sand cleaning mechanism 200, including a filter plate 201 and a sand cleaning assembly 202.

[0035] See Figure 3 , Figure 4 , Figure 5 , Figure 7 and Figure 8 As shown, the filter plate 201 has a plurality of V-shaped plates connected in parallel along the vertical direction, which can realize the accumulation of sand and stones at the corners of the V-shaped plates, and a plurality of filter holes 201b are arranged in an array on the V-shaped plates, and the filter holes 201b are used to filter impurities in the water. A first outer protective frame 201a extending backward and having an L-shaped vertical cross section is fixed on the outer side of the filter plate 201, and a filter window 101b-2 is opened on the box cover 101b, and the first outer protective frame 201a is fixedly connected to the filter window 101b-2 by bolts, and this arrangement can realize the removal and replacement of the filter plate 201; A U-shaped frame 101b-3 with an upper opening is fixedly arranged on one side of the box cover 101b close to the outer protective box 101a, and a slot 101b-4 for slidingly inserting with the matching frame 101e-1 is opened on the inner side wall of the U-shaped frame 101b-3, and a collection bag 101e is fixedly arranged on the inner side wall of the matching frame 101e-1, which is convenient for disassembly and replacement of the collection bag 101e; a water outlet pipe 101c is fixedly arranged on the rear side of the outer protective box 101a, and a water pump 101c-1 is fixedly installed on the water outlet pipe 101c, and the arrangement of the water pump 101c-1 can realize the discharge of water inside the outer protective box 101a; When the above arrangement is in use, the water outlet pipe 101c can extract the water inside the outer protective box 101a through the operation of the water pump 101c-1. Under this action, water and DNA information enter from the filter hole 201b on the filter plate 201, and after being collected by the collection bag 101e, the water is finally discharged from the water outlet pipe 101c, and the DNA sample is collected in the collection bag 101e.

[0036] Furthermore, a first sealing groove 101a-3 is opened on the front side surface of the outer protective box 101a, and a sealing frame 101b-1 for gap fitting with the first sealing groove 101a-3 is fixedly provided on one side surface of the box cover 101b to achieve the sealing between the box cover 101b and the outer protective box 101a. In actual use, a rubber ring can be arranged between the sealing frame 101b-1 and the first sealing groove 101a-3 to improve the sealing.

[0037] See Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 and Fig. 9 As shown, the sand cleaning component 202 includes a screw rod 202b, a first bevel gear 202c and a triangular block 202e. The inner concave surface of each V-plate is fitted with a triangular block 202e. V-shaped scrapers 202e-1 are symmetrically fixed on both side surfaces of the triangular block 202e, and the scrapers 202e-1 are fitted with the inner concave surface of the V-plate. The cross-section of the scraper 202e-1 is triangular. The arrangement of the two scrapers 202e-1 can always clean the sand and stones on the filter plate 201 during the reciprocating movement of the triangular block 202e. A connector 202e-2 is fixed in the middle of one side of the triangular block 202e. Multiple connectors 202e-2 are fixedly connected by a linkage rod 202e-3. The upper end of the linkage rod 202e-3 is fixedly connected to one end of the driving plate 202e-5 by a connecting rod 202e-4. Then, the driving plate 202e-5 is spirally fitted on the screw rod 202b, the screw rod 202b is arranged above the outer protective box 101a, and both ends of the screw rod 202b are rotatably sleeved on the support plate 202b-2 through rolling bearings, and the support plate 202b-2 is fixedly connected to the top surface of the outer protective box 101a by bolts, and the two ends of the screw rod 202b are symmetrically fixed with third bevel gears 202b-1, one of the third bevel gears 202b-1 is meshed with the first bevel gear 202c below, and the bottom surface of the first bevel gear 202c is fixed with a second rotating column 202c-1, and the other end of the second rotating column 202c-1 slides through the top plate of the outer protective box 101a and is embedded in the output end of the motor 202c-4, and the motor 202c-4 is fixed on the inner top surface of the outer protective box 101a; A sealing ring 202c-2 is fixedly sleeved on the side wall of the second rotating column 202c-1, and an oil seal groove 202c-3 is opened on the outer wall of the sealing ring 202c-2 in the circumferential direction. The oil seal groove 202c-3 is filled with sealing oil to improve the sealing performance between the sealing ring 202c-2 and the outer protective box 101a. A second sealing groove 101a-4 for clearance fit of the sealing ring 202c-2 is opened on the top plate of the outer protective box 101a; a guide block 202e-6 is fixedly provided at the other end of the driving plate 202e-5 to which the linkage rod 202e-3 is fixed, and a guide groove 101a-2 for clearance fit of the guide block 202e-6 is opened on the top surface of the outer protective box 101a along the axial direction of the screw rod 202b. This arrangement can play a role of limiting and guiding the movement of the driving plate 202e-5; When the above-mentioned arrangement is in use, the operation of the motor 202c-4 can realize that the second rotating column 202c-1 drives the first bevel gear 202c to rotate. Because the first bevel gear 202c and the third bevel gear 202b-1 are meshed, under the rotation of the first bevel gear 202c, the third bevel gear 202b-1 can drive the screw rod 202b to rotate. Because of the spiral matching arrangement between the screw rod 202b and the driving plate 202e-5, the linkage rod 202e-3 can finally drive multiple triangular blocks 202e to reciprocate in the horizontal direction. The reciprocating movement of the triangular blocks 202e on the outside of the filter plate 201 can realize the cleaning of sand and gravel on the surface of the filter plate 201.

[0038] Furthermore, a waterproof cover 101f is fixedly connected to the inner top surface of the outer protective box 101a by bolts. The setting of the waterproof cover 101f can form an independent waterproof space above the inner part of the outer protective box 101a for waterproofing various electrical components. In actual use, the waterproof cover 101f is used for the installation and placement of batteries, controllers, etc. The batteries can supply power to various electrical components, while the controller can control various electrical components. The controller can also be wirelessly connected to the control terminal, thereby facilitating remote centralized control by staff. Example 2

[0039] Reference Figure 6 , Fig. 9 and Fig.10 , which is the second embodiment of the present invention, is based on the previous embodiment, except that, in order to reduce the energy consumption of the motor 202c-4 when in use, water flow is used to assist in driving the screw rod 202b to rotate, thereby achieving energy saving.

[0040] Specifically, the sand cleaning component 202 also includes a water fan blade 202a and a mounting box 202d. A first rotating column 202a-1 is fixedly arranged on the bottom surface of the water fan blade 202a. The lower end of the first rotating column 202a-1 slides through the top plate of the outer protective box 101a and is fixedly connected to the I-shaped disk 203a-3. A second bevel gear 202a-2 that meshes with the third bevel gear 202b-1 is symmetrically fixedly arranged on the first rotating column 202a-1 above and below the third bevel gear 202b-1. The groove position of the I-shaped disk 203a-3 is slidably sleeved with a linkage plate 202d-1. On the one hand, this setting can realize that the linkage plate 202d-1 drives the I-shaped disk 203a-3 to move up and down, and on the other hand, the I-shaped disk 203a-3 can rotate freely relative to the linkage plate 202d-1. The two ends of the linkage plate 202d-1 are symmetrically slidably sleeved with T-shaped columns 202d-4. This setting can play a role in limiting and guiding the movement of the linkage plate 202d-1, and the T-shaped column 202d-4 above the linkage plate 202d-1 is slidably sleeved with a spring 20 2d-5, spring 202d-5 can reset the position of linkage plate 202d-1, a magnetic plate 202d-2 is fixedly arranged on the bottom surface of linkage plate 202d-1 between I-shaped disk 203a-3 and T-shaped column 202d-4, the installation box 202d is covered on the outside of linkage plate 202d-1, and the installation box 202d is fixedly connected to the inner top surface of outer protective box 101a by bolts, an electromagnetic plate 202d-3 is fixedly arranged on the bottom surface of installation box 202d, and the side surface close to electromagnetic plate 202d-3 and magnetic plate 202d-2 is arranged in the same sex; When the above arrangement is in use, the electromagnetic plate 202d-3 can generate magnetic force by energizing the electromagnetic plate 202d-3. Because the electromagnetic plate 202d-3 and the side surface close to the magnet plate 202d-2 are arranged in the same manner, combined with the reset of the spring 202d-5, the linkage plate 202d-1 can drive the first rotating column 202a-1 to adjust its position in the vertical direction through the I-shaped disk 203a-3, and the two second bevel gears 202a-2 are arranged symmetrically, so that the two second bevel gears 202a-2 can be adjusted. When the wheel 202a-2 is engaged with the third bevel gear 202b-1, the rotation direction of the screw rod 202b is not the same. In this way, in actual use, when the water flow drives the water fan blade 202a to rotate, the second bevel gear 202a-2 drives the third bevel gear 202b-1 to rotate, and the rotation direction of the screw rod 202b driven by the motor 202c-4 is always consistent with the rotation direction of the screw rod 202b driven by the above-mentioned second bevel gear 202a-2, thereby reducing the energy consumption of the motor 202c-4.

[0041] Furthermore, a detection groove 202a-4 is provided on the side wall of the first rotating column 202a-1 inside the installation box 202d along the circumferential direction, and the diameter of the detection groove 202a-4 is gradually increased or decreased from one end to the other end, and a distance sensor 202d-6 is fixedly sleeved on the side wall of the installation box 202d on one side of the detection groove 202a-4, so that the law of the depth change of the detection groove 202a-4 can be detected by the distance sensor 202d-6 to determine how the water flow drives the water fan blade 202a to rotate; In addition, it should be noted that when the water flow is small, the water fan blades 202a may not be driven by the water flow to rotate by themselves. Even in this case, when the motor 202c-4 drives the screw rod 202b to rotate, the water fan blades 202a can still rotate along the direction of the water flow to reduce energy consumption. The actual flow direction of the water flow can also be determined by detecting the time when the depth of the detection slot 202a-4 changes. Example 3

[0042] Reference Figure 1 , Figure 3 , Figure 4 , Figure 7 , Fig.10 , Fig.11 and Fig.12 , which is the third embodiment of the present invention, is based on any of the above embodiments, except that, in order to improve the richness of water sample information collection, the height area of ​​the collection box 101 is adjusted through the adjustment mechanism 300.

[0043] Specifically, it also includes an adjustment mechanism 300, which includes an adjustment airbag 301 and a first conveying pipe 302. A U-shaped groove 101g is provided at the lower end of the outer protective box 101a at the rear side of the sand cleaning mechanism 200. The adjustment airbag 301 is U-shaped and is arranged in the U-shaped groove 101g. Second outer protective frames 301b are symmetrically fixed on both sides of the adjustment airbag 301, and the two upper ends of the two second outer protective frames 301b are respectively fixedly connected by extension plates 301b-1. A connecting strip 101a-1 is fixedly provided on the outer side wall of the outer protective box 101a at the upper end of the U-shaped groove 101g, and the connecting strip 101a-1 and the extension plate 301b-1 are fixedly connected by bolts to realize the detachable connection of the adjustment airbag 301. The upper ends of both sides of the regulating airbag 301 are symmetrically fixed with three-way pipes 301a, and the tube body connected to the regulating airbag 301 in the three-way pipe 301a is fixedly installed with a second solenoid valve 301a-1 to realize the on and off of the three-way pipe 301a, and the three-way pipes 301a on two adjacent regulating airbags 301 that are aligned vertically are fixedly connected through a first delivery pipe 302, and the first delivery pipe 302 realizes the delivery of gas; A straight pipe 102c is symmetrically fixedly connected to the suspension board 102, and the straight pipe 102c and the three-way pipe 301a are arranged in a one-to-one correspondence. The three-way pipe 301a on the uppermost regulating airbag 301 is connected to the corresponding straight pipe 102c through the first delivery pipe 302. A first solenoid valve 102c-1 is fixedly installed on the straight pipe 102c to realize the on-off of the straight pipe 102c. The upper end of one straight pipe 102c is connected to the output end of the air pump 102e through the second delivery pipe 102e-1, and the air pump 102e is fixedly installed on the top surface of the suspension board 102. In actual use, the straight pipe 102c that is not connected to the air pump 102e can be auxiliaryly connected to an exhaust pipe to be buoyant to prevent water from entering from the straight pipe 102c; When the above-mentioned arrangement is in use, the first solenoid valve 102c-1 on a straight tube 102c that is not connected to the air pump 102e is opened, and the second solenoid valve 301a-1 on a three-way tube 301a on the regulating airbag 301 that needs to be exhausted is opened, so that the gas in the regulating airbag 301 on the specific collection box 101 can be discharged. When the first solenoid valve 102c-1 on a straight tube 102c connected to the air pump 102e is opened, and the second solenoid valve 301a-1 on a three-way tube 301a on the regulating airbag 301 that needs to be inflated is opened, so that the gas in the regulating airbag 301 on the specific collection box 101 can be injected. In this way, the buoyancy of the collection box 101 in the water can be adjusted by adjusting the amount of gas inside the regulating airbag 301, thereby adjusting the height of the collection box 101 in the water.

[0044] Furthermore, a plug post 301b-2 is fixedly provided at a corner of the second outer protective frame 301b, and a plug hole 101g-1 for slidingly inserting the plug post 301b-2 is provided on the outer protective box 101a, so as to improve the stability of the cooperation between the second outer protective frame 301b and the outer protective box 101a.

[0045] Furthermore, internally threaded sleeves 101d are symmetrically fixed on the two symmetrical side walls of the outer protective box 101a, an internally threaded blind sleeve 102b is fixed on the bottom surface of the suspension board 102, and a traction rope 103 is provided between the two adjacent internally threaded sleeves 101d and between the internally threaded sleeve 101d and the internally threaded blind sleeve 102b. Threaded columns 103a are symmetrically fixed on both ends of the traction rope 103, one of the threaded columns 103a is spirally fitted on the internally threaded sleeve 101d, and the other threaded column 103a is spirally sleeved on the internally threaded sleeve 101d or the internally threaded blind sleeve 102b. The traction rope 103 can realize a detachable fitting connection between the collection boxes 101 and between the collection box 101 and the suspension board 102.

[0046] Furthermore, a suspension air bag 102a is fixedly provided on the bottom surface of the suspension board 102 to make the suspension board 102 float on the water surface. The four corners of the suspension board 102 are provided with mounting holes 102d, which are convenient for connection and fixation with the lifting device. Example 4

[0047] This embodiment is the fourth embodiment of the present invention, which provides a method for collecting DNA from a sandy water environment. Specifically, the collection operation is performed according to the following steps: S1: Select a corresponding number of collection boxes 101 according to the water depth and the richness of the collected samples, and fix the multiple collection boxes 101 and the collection boxes 101 and the suspension board 102 together through traction ropes 103; S2: Lower the collection mechanism 100 to a designated area manually or by a lifting device; S3: The collection mechanism 100 collects the water environment DNA. When the collection reaches the predetermined time, the sand cleaning component 202 is controlled to work to clean the sand outside the filter plate 201, and then the sand cleaning is repeated at a predetermined time period; S4: During the collection process, the air pump 102e, the first solenoid valve 102c-1 and the second solenoid valve 301a-1 are controlled to work in coordination to inflate and deflate the regulating airbag 301, thereby adjusting the position of the collection box 101 in the vertical direction; S5: After the collection is completed, the collection mechanism 100 is salvaged manually or by a lifting device, and the staff can collect and store the samples in the collection bag 101e.

[0048] In addition, it should be noted that the components not described in detail in this article are prior art.

[0049] Importantly, it should be noted that the construction and arrangement of the present application shown in a plurality of different exemplary embodiments are only exemplary. Although only a few embodiments are described in detail in this disclosure, it should be readily understood by those who refer to this disclosure that many modifications are possible (e.g., the size, scale, structure, shape and ratio of various elements, and parameter values ​​(e.g., temperature, pressure, etc.), installation arrangements, use of materials, color, directional changes, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in the application. For example, the element shown as integrally formed can be composed of a plurality of parts or elements, the position of the element can be inverted or otherwise changed, and the nature or number or position of the discrete element can be changed or changed. Without departing from the scope of the present invention, other replacements, modifications, changes and omissions can be made in the design, operating conditions and arrangement of the exemplary embodiments. Therefore, the present invention is not limited to a specific embodiment, but extends to a variety of modifications that still fall within the scope of the appended claims.

[0050] Additionally, in order to provide a concise description of exemplary embodiments, all features of an actual embodiment may not be described (ie, those features that are not relevant to the best mode presently contemplated for carrying out the invention or those features that are not relevant to implementing the invention).

[0051] It will be appreciated that in the development of any actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will be a routine task of design, fabrication, and production for those of ordinary skill having the benefit of this disclosure without undue experimentation.

[0052] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A DNA collection device for sandy water environment, characterized by: include, The collection mechanism (100) comprises collection boxes (101) arranged in an array along a vertical direction, a suspension board (102) arranged above the collection boxes (101), and a traction rope (103) used to connect the collection boxes (101) or between the collection boxes (101) and the suspension board (102), wherein the collection box (101) comprises an outer protective box (101a) and a box cover (101b) fixedly connected to the front end of the outer protective box (101a) by bolts; and, The sand cleaning mechanism (200) comprises a filter plate (201) and a sand cleaning assembly (202), wherein the filter plate (201) comprises a plurality of V-shaped plates arranged in parallel in a vertical direction, and the V-shaped plates are provided with a plurality of filter holes (201b) in an array, a first outer protective frame (201a) extending backward and having an L-shaped vertical cross section is fixedly provided on the outer side of the filter plate (201), a filter window (101b-2) is provided on the box cover (101b), and the first outer protective frame (201a) is fixedly connected to the filter plate by bolts. In the window (101b-2), the sand cleaning component (202) comprises a screw rod (202b), a first bevel gear (202c) and a triangular block (202e), the inner concave surface of each V-shaped plate is fitted with a triangular block (202e), a connecting head (202e-2) is fixedly provided in the middle of one side surface of the triangular block (202e), a plurality of connecting heads (202e-2) are fixedly connected via a linkage rod (202e-3), and the upper end of the linkage rod (202e-3) is connected via a connecting rod (20 The drive plate (202e-5) is screw-fitted on the screw rod (202b), the screw rod (202b) is arranged above the outer protective box (101a), and both ends of the screw rod (202b) are rotatably sleeved on the support plate (202b-2) via rolling bearings, the support plate (202b-2) is fixedly connected to the top surface of the outer protective box (101a) via bolts, and the two ends of the screw rod (202b) are symmetrically fixed. A third bevel gear (202b-1) is provided, wherein one of the third bevel gears (202b-1) meshes with the first bevel gear (202c) below, and a second rotating column (202c-1) is fixedly provided on the bottom surface of the first bevel gear (202c), while the other end of the second rotating column (202c-1) slides through the top plate of the outer protective box (101a) and is embedded in the output end of the motor (202c-4), and the motor (202c-4) is fixedly provided on the inner top surface of the outer protective box (101a).

2. The DNA collection device for sandy water environment as claimed in claim 1, characterized in that: V-shaped scrapers (202e-1) are symmetrically fixed on both side surfaces of the triangular block (202e), and the scrapers (202e-1) fit the inner concave surface of the V-shaped plate. The cross section of the scrapers (202e-1) is triangular.

3. A DNA collection device for sandy water environment as claimed in claim 2, characterized in that: A sealing ring (202c-2) is fixedly sleeved on the side wall of the second rotating column (202c-1), an oil seal groove (202c-3) is circumferentially provided on the outer side wall of the sealing ring (202c-2), and a second sealing groove (101a-4) for clearance fit of the sealing ring (202c-2) is provided on the top plate of the outer protective box (101a); A guide block (202e-6) is fixedly provided at the other end of the driving plate (202e-5) on which the linkage rod (202e-3) is fixedly provided, and a guide groove (101a-2) for clearance fit of the guide block (202e-6) is provided on the top surface of the outer protective box (101a) along the axial direction of the screw rod (202b).

4. The DNA collection device for sandy water environment as claimed in claim 2, characterized in that: A U-shaped frame (101b-3) with an upper end opening is fixedly provided on a side surface of the box cover (101b) close to the outer protective box (101a); a slot (101b-4) for slidingly inserting the matching frame (101e-1) is provided on the inner side wall of the U-shaped frame (101b-3); and a collection bag (101e) is fixedly provided on the inner side wall of the matching frame (101e-1); A water outlet pipe (101c) is fixedly provided on the rear side surface of the outer protective box (101a), and a water pump (101c-1) is fixedly installed on the water outlet pipe (101c).

5. The DNA collection device for sandy water environment as claimed in claim 4, characterized in that: A first sealing groove (101a-3) is provided on the front side of the outer protective box (101a), and a sealing frame (101b-1) for gap fitting of the first sealing groove (101a-3) is fixedly provided on one side surface of the box cover (101b); A water-blocking cover (101f) is fixedly connected to the inner top surface of the outer protective box (101a) by means of bolts.

6. A DNA collection device for sandy water environment as claimed in claim 1, 3 or 5, characterized in that: The sand clearing component (202) further comprises a water fan blade (202a) and a mounting box (202d); a first rotating column (202a-1) is fixedly provided on the bottom surface of the water fan blade (202a); the lower end of the first rotating column (202a-1) slides through the top plate of the outer protective box (101a) and is fixedly connected to the I-shaped disk (203a-3); and a second bevel gear (202a-2) meshing with the third bevel gear (202b-1) is symmetrically fixedly provided on the first rotating column (202a-1) above and below the third bevel gear (202b-1); A linkage plate (202d-1) is slidably sleeved at the groove position of the I-shaped plate (203a-3), T-shaped columns (202d-4) are symmetrically slidably sleeved at both ends of the linkage plate (202d-1), and a spring (202d-5) is slidably sleeved on the T-shaped column (202d-4) above the linkage plate (202d-1), and the linkage plate (202d-5) between the I-shaped plate (203a-3) and the T-shaped column (202d-4) is slidably sleeved. d-1) a magnetic plate (202d-2) is fixedly provided on the bottom surface, the installation box (202d) is covered on the outside of the linkage plate (202d-1), and the installation box (202d) is fixedly connected to the inner top surface of the outer protective box (101a) by bolts, an electromagnetic plate (202d-3) is fixedly provided on the bottom surface of the installation box (202d), and the side surfaces of the electromagnetic plate (202d-3) and the magnetic plate (202d-2) that are close to each other are arranged in the same manner; A detection groove (202a-4) is provided on the side wall of the first rotating column (202a-1) inside the installation box (202d) along the circumferential direction, and the diameter of the detection groove (202a-4) is gradually increased or decreased from one end to the other end, and a distance measuring sensor (202d-6) is fixedly sleeved on the side wall of the installation box (202d) on one side of the detection groove (202a-4).

7. The DNA collection device for sandy water environment as claimed in claim 6, characterized in that: It also comprises an adjustment mechanism (300), the adjustment mechanism (300) comprising an adjustment airbag (301) and a first conveying pipe (302); a U-shaped groove (101g) is provided at the lower end of the outer protective box (101a) at the rear side of the sand cleaning mechanism (200); the adjustment airbag (301) is U-shaped and is arranged in the U-shaped groove (101g); second outer protective frames (301b) are symmetrically fixedly provided on both sides of the adjustment airbag (301); the two upper ends of the two second outer protective frames (301b) are respectively fixedly connected by an extension plate (301b-1); a connecting strip (101a-1) is fixedly provided on the outer side wall of the outer protective box (101a) at the upper end of the U-shaped groove (101g); and the connecting strip (101a-1) and the extension plate (301b-1) are fixedly connected by bolts; The upper ends of both sides of the regulating airbag (301) are symmetrically fixed with three-way pipes (301a), and a second solenoid valve (301a-1) is fixedly installed on a tube body in the three-way pipe (301a) connected to the regulating airbag (301), and the three-way pipes (301a) on two adjacent regulating airbags (301) that are aligned vertically are fixedly connected via a first delivery pipe (302); The suspension plate (102) is symmetrically and fixedly connected with a straight tube (102c), and the straight tube (102c) and the three-way tube (301a) are arranged in a one-to-one correspondence, the three-way tube (301a) on the uppermost regulating airbag (301) is connected to the corresponding straight tube (102c) via a first delivery tube (302), a first solenoid valve (102c-1) is fixedly mounted on the straight tube (102c), the upper end of one of the straight tubes (102c) is connected to the output end of the air pump (102e) via a second delivery tube (102e-1), and the air pump (102e) is fixedly mounted on the top surface of the suspension plate (102).

8. The DNA collection device for sandy water environment as claimed in claim 7, characterized in that: The second outer protective frame (301b) is fixedly provided with an insertion column (301b-2) at a corner position, and the outer protective box (101a) is provided with an insertion hole (101g-1) for sliding insertion of the insertion column (301b-2).

9. The DNA collection device for sandy water environment as claimed in claim 8, characterized in that: Internally threaded sleeves (101d) are symmetrically fixed on two symmetrical side walls of the outer protective box (101a); an internally threaded blind sleeve (102b) is fixed on the bottom surface of the suspension plate (102); a traction rope (103) is provided between two upper and lower adjacent internally threaded sleeves (101d) and between the internally threaded sleeve (101d) and the internally threaded blind sleeve (102b); threaded posts (103a) are symmetrically fixed at both ends of the traction rope (103); one of the threaded posts (103a) is spirally fitted on the internally threaded sleeve (101d), and the other threaded post (103a) is spirally sleeved on the internally threaded sleeve (101d) or the internally threaded blind sleeve (102b); A suspension air bag (102a) is fixedly arranged on the bottom surface of the suspension board (102), and mounting holes (102d) are provided at the four corners of the suspension board (102).

10. A method for collecting DNA from a sandy water environment, characterized in that: The DNA collection device for a sandy water environment according to any one of claims 7 to 9 performs a collection operation according to the following steps; S1: selecting a corresponding number of collection boxes (101) according to the water depth and the richness of the collected samples, and fixing the multiple collection boxes (101) and the collection boxes (101) and the suspension board (102) together through traction ropes (103); S2: lowering the collection mechanism (100) to a designated area manually or using a lifting device; S3: the collection mechanism (100) collects the water environment DNA, and when the collection reaches a predetermined time, the sand removal component (202) is controlled to work to remove sand from the outside of the filter plate (201), and the sand removal is repeated at a predetermined time period; S4: During the collection process, the air pump (102e), the first solenoid valve (102c-1) and the second solenoid valve (301a-1) are controlled to work in coordination so as to inflate and deflate the regulating airbag (301), thereby adjusting the position of the collection box (101) in the vertical direction; S5: After the collection is completed, the collection mechanism (100) is salvaged manually or by a lifting device, and the staff can collect and store the samples in the collection bag (101e).

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