Handheld clamping strip detection device

Abstract

The invention provides a handheld card strip detection device which can detect two kinds of card strips, namely an immunofluorescence chromatography card strip and a colloidal gold card strip, and a shell of the device integrates three main parts, namely a mobile positioning part, a photoelectric detection part and a circuit part. The technical problems that in a traditional card strip detection method, the size of a device is too large, cost is too high, and a detection result depends on naked eye judgment are solved, a device shell is arranged to be in a shape suitable for being held by hand, the size is small, and a user only needs to manually push a card strip into equipment; detection can be completed by means of cooperative work of the mobile positioning part and the photoelectric detection part, a detection result can be visually displayed on a display screen, an accurate detection result can be obtained in real time, the detection speed and accuracy are improved, the cost is reduced, in addition, a sliding cover arranged on the outer surface of the device shell is matched with a replaceable optical module, and the detection accuracy is improved. The universal detection of the immunofluorescence chromatography card strip marked by different fluorescent substances is realized, and the requirement on the compatibility of the card strip in clinical detection can be met.

Description

Technical Field

[0001] The invention relates to the field of detection instruments, and in particular to a device for performing portable, compatible and light source replaceable detection on two types of card strips, namely, an immunofluorescence chromatography card strip and a colloidal gold card strip. Background Art

[0002] In modern medicine, Point-of-Care Testing (POCT) technology plays an important role in clinical diagnosis because it can quickly provide test results. As common POCT tools, immunofluorescence chromatography cards and colloidal gold cards are widely used to quickly detect specific markers in biological samples such as blood and urine. Both cards are generally equipped with test lines (T lines) and control lines (C lines) to capture and detect the test objects and verify the effectiveness of the test process. Immunofluorescence chromatography cards are specifically a combination of immunochromatography technology and fluorescent labeling technology. They use fluorescent substances as markers and make quantitative or qualitative judgments based on the strength of the fluorescent signal formed by the fluorescent complex fixed by the captured antibody on the test line; colloidal gold cards use nanogold particles as markers and make qualitative judgments by observing the color changes (such as red) on the test line with the naked eye.

[0003] The peak ratio usually refers to the ratio between the signal intensity on the test line (T line) and the signal intensity on the control line (C line). In card strip testing, the detection of the "peak ratio" is a key technical indicator, the purpose of which is to improve the accuracy and reliability of the test results. By calculating this ratio, the signal intensity fluctuations caused by changes in the external environment (such as light intensity, temperature, etc.) and individual differences in operators can be effectively corrected. In addition, the peak ratio can also help quantify the test results, so that even weak signal changes can be accurately captured, which not only improves the sensitivity of the test, but also helps to standardize the test results, thereby reducing errors in subjective judgment and strengthening quality control of the test process. However, when detecting immunofluorescence chromatography card strips, a time judgment method is usually used to judge the position of the card slot entering the instrument, thereby determining the position of the card strip during the detection process. The principle of the time judgment method is based on the fact that the time for each card slot to be pushed and moved is fixed, and the position of the card slot is judged by a pre-set fixed time interval. The specific steps include: presetting the time interval required for each card slot to be pushed, manually or mechanically pushing and moving the card slot, using a timer to record the time for pushing the card slot, and finally judging the position of the card slot by recording the time interval. The time judgment method does not require additional sensors and has low cost. However, since the speed of the card slot advancement may change due to uncertain reasons such as human or mechanical factors, relying solely on time to judge the position will cause the fluorescence curve to shift, thereby affecting the accuracy of the detection result. At the same time, when the advancement speed is unstable, the time judgment method cannot provide accurate position feedback, resulting in a change in the distance between the T line (detection line) and the C line (control line) on the card strip, which in turn leads to incorrect peak search and peak value ratios, further affecting the accuracy and consistency of the detection results. In addition, in order to ensure the accuracy of the detection, the advancement speed needs to be strictly controlled, which will also increase the complexity and difficulty of the operation and thus reduce the reliability of the detection. Traditional colloidal gold card strips use the naked eye to judge the test results, which is easily affected by ambient light and personal subjective factors, resulting in great potential errors or risks of misjudgment.

[0004] There are also some disadvantages in the detection instruments of these two types of card strips. First, the current reading devices for compatibility detection of the two types of card strips are usually large in size and not suitable for mobile medical or home use. Secondly, the reading devices for compatibility detection of the two types of card strips need to meet the integration of the acquisition and processing functions of two different signals, and the layout of the functional modules is relatively complex, and the price is relatively high, which limits their application in a wider range. Finally, the operation of some equipment is relatively complicated, and it requires certain training to use it accurately, which is also a major challenge. These shortcomings limit the popularity of traditional card strip detection technology in practical applications, especially in areas with limited resources. Therefore, it is imperative to develop a more portable, economical, easy-to-operate, efficient and easy-to-use device or apparatus for compatibility detection of the two types of card strips to solve the problems existing in the prior art such as large equipment size, high cost, and complex operation. In addition, common fluorescent markers on immunofluorescence chromatography cards include fluorescein, quantum dots, and upconversion nanoparticles. Among them, fluorescein fluorescent markers require a shorter wavelength of 365nm ultraviolet light source for excitation. The excitation light source range configured in common fluorescence detectors is generally between 300nm and 370nm, which can be used for the detection of corresponding immunofluorescence chromatography cards. However, when the fluorescent markers are quantum dots and upconversion nanoparticles, they cannot be used for detection: quantum dots require a wider wavelength range of excitation light, including the wavelength range of visible light, while upconversion nanoparticles require a long-wavelength excitation light source, such as near-infrared light. Common excitation wavelengths include 808nm, 980nm, etc. If the excitation light required by the immunofluorescence chromatography card is within the visible light range, how to achieve compatibility detection with the colloidal gold card; if the immunofluorescence chromatography card requires a shorter or longer wavelength of excitation light, how to achieve the best detection; therefore, it is also particularly important to develop a more portable, economical and easy-to-operate card detection device to meet the needs of diversity of immunofluorescence chromatography cards. Summary of the invention

[0005] The purpose of the present invention is to overcome the defects of the existing card strip detection technology and provide a handheld card strip detection device which is easy to carry, has a simple structure, a small size, a low cost, a simple operation, a short detection time, is flexible to use, and has a high detection accuracy, and can be used to perform compatibility detection on different types of card strips.

[0006] To achieve the above purpose, the present invention provides the following technical solutions: In the first aspect, the present invention provides a handheld card strip detection device, which can simultaneously detect two types of card strips, namely immunofluorescence chromatography card strips and colloidal gold card strips, for compatibility and portable detection. The shell of the device is set to a shape suitable for handholding, and is composed of an upper shell part and a lower shell part. The functional components arranged on the outer surface and inside of the shell of the device include a mobile positioning part, a photoelectric detection part and a circuit part. A display screen and a switch for realizing human-computer interaction are also arranged on the outer surface of the shell of the device; the mobile positioning part is used to control the card strip to be pushed smoothly into the device for detection; the photoelectric detection part is used to emit light and detect the collected light signal on the card strip; the circuit part is used to provide power supply and data transmission for the device; the mobile positioning part includes a card slot for placing the card strip, which is used to provide a linear guide rail and a fixed sliding track A slider, and an encoder for ensuring stable sliding movement and precise alignment; a photoelectric detection part is used to detect the optical signal on the card strip, including an optical module, an optical module shielding shell and an optical module fixing block, the optical module is installed in the optical module shielding shell, and the optical module shielding shell is fixed on the optical module fixing block; two optical paths are formed in the optical module, namely a transmitting optical path and a receiving optical path, the light source is arranged in the light source fixing groove, the photoelectric sensor is arranged in the photoelectric sensor fixing groove, the transmitting optical path is used to irradiate the light emitted by the light source from the light source fixing groove to the card strip, and the receiving optical path is used to emit the optical signal on the card strip from the light exit hole to the photoelectric sensor fixing groove, and the optical signal is collected by the photoelectric sensor fixed in the photoelectric sensor fixing groove for detection; a circuit part includes a main control board, a battery, a power supply board and a power interface that can realize a dual power supply mode.

[0007] According to a preferred embodiment of the present invention, the mobile positioning part also includes an optical coupler, a slider, a damping rack, a rotating shaft, a code disc, a gear and related fixed structures for providing precise control and smooth movement of the linear guide. The optical coupler is used to ensure the accuracy of signal transmission, the slider is used to ensure the smoothness and stability of the movement of the linear guide, the damping rack increases the damping coefficient of the slider, and ensures the stability of signal acquisition. The coordinated cooperation of the rotating shaft, the code disc and the gear ensures the stable connection and precise alignment of the moving components.

[0008] Preferably, the fixing structure includes an optical coupler fixing block, a right-turn bearing seat, a left-turn bearing seat, a rotating bearing seat fixing block, a damping rack fixing block, and an encoder fixing block, wherein the optical coupler fixing block is used to fix the optical coupler; the code disc and the gear are installed on the rotating shaft, the right-turn bearing seat and the left-turn bearing seat are used to fix the rotating shaft, the optical coupler fixing block and the rotating bearing seat fixing block respectively fix the right-turn bearing seat and the left-turn bearing seat; the damping rack fixing block is used to fix the damping rack; the encoder fixing block is used to fix the encoder.

[0009] According to a preferred embodiment of the present invention, in the photoelectric detection part, the optical module fixing block includes an upper part of the optical module fixing block and a lower part of the optical module fixing block, the upper part of the optical module fixing block is used to fix the optical module shielding shell, the lower part of the optical module fixing block is fixed to the bottom of the lower half of the shell, and the upper part of the optical module fixing block and the lower part of the optical module fixing block are fixedly connected.

[0010] Preferably, the upper portion of the optical module fixing block and the lower portion of the optical module fixing block are fixedly connected to form an inverted "L" shape.

[0011] According to a preferred embodiment of the present invention, among the two optical paths, the transmitting optical path is formed by a light source, a light source fixing groove, a filter, a metal slit, an transmitting lens and a light entrance hole; the receiving optical path is formed by a light exit hole, a receiving lens, a wide-diameter filter, a photoelectric sensor fixing groove and a photoelectric sensor.

[0012] Preferably, the number of metal slits and emitting lenses is set to 1-3.

[0013] Further preferably, the number of the metal slits and the emitting lenses is 2, and the two emitting lenses are arranged between the two metal slits.

[0014] According to a preferred embodiment of the present invention, in the photoelectric detection part, the light source is an LED light source, and the photoelectric sensor is a photodiode.

[0015] Preferably, in the photoelectric detection part, the LED light source is a white LED and / or a green LED.

[0016] According to a preferred embodiment of the present invention, a slidable sliding cover is provided on the outer surface of the upper half of the housing, and the optical module is a replaceable structure.

[0017] According to a preferred embodiment of the present invention, the material of the housing of the device can be selected from resin, plastic, aluminum alloy or carbon fiber.

[0018] Preferably, the color of the outer shell of the device is black, so as to avoid interference of external light sources on the detection results to the greatest extent.

[0019] According to a preferred embodiment of the present invention, in the movable positioning part, the card slot is provided with a universal card strip insertion track on which both immunofluorescence chromatography card strips and colloidal gold card strips can be smoothly placed.

[0020] According to a preferred embodiment of the present invention, the power supply current of the light source is controlled by the circuit part.

[0021] Preferably, when detecting immunofluorescence chromatography card strips, the power supply current is preferably controlled to be 90-110 mA; when detecting colloidal gold card strips, the power supply current is preferably controlled to be 4-6 mA.

[0022] According to a preferred embodiment of the present invention, in the photoelectric detection part, a layer of copper foil is attached to the outer surface of the optical module shielding shell.

[0023] According to a preferred embodiment of the present invention, in the photoelectric detection part, the filter is preferably a square with a side length of 3-7 mm, and can be a green filter or a red filter.

[0024] Preferably, a green filter is preferred when the light source is a green LED.

[0025] According to a preferred embodiment of the present invention, in the photoelectric detection part, the filter is preferably fixed in the optical module in a plug-in manner.

[0026] Preferably, when detecting immunofluorescence chromatography card strips, no filter may be provided; when detecting colloidal gold card strips, the filter is plugged and installed in the optical module.

[0027] According to a preferred embodiment of the present invention, in the photoelectric detection part, the wide-diameter filter is relatively wider than the filter, preferably circular or semicircular with a diameter of 7.5-10.5 mm.

[0028] According to a preferred embodiment of the present invention, in the photoelectric detection part, the transmitting lens and the receiving lens are preferably semicircular with a diameter of 7.5-10.5 mm.

[0029] According to a preferred embodiment of the present invention, in the photoelectric detection part, the transmitting lens and the receiving lens are preferably fixed in the optical module in a plug-in manner.

[0030] According to a preferred embodiment of the present invention, the photoelectric sensor is fixed above the optical module through a photoelectric sensor fixing groove.

[0031] According to a preferred embodiment of the present invention, the optical module is a light-proof structure.

[0032] According to a preferred embodiment of the present invention, the optical module can switch and control the wavelength of light emitted by the light source; in the photoelectric detection part, when detecting immunofluorescence chromatography card strips, a white LED is preferably used as the light source; when detecting colloidal gold card strips, a green LED with a wavelength of 515-525 nanometers is preferably used as the light source.

[0033] According to a preferred embodiment of the present invention, in the circuit part, the battery is a rechargeable battery, and in conjunction with the power interface, a dual power supply mode of battery power supply or direct power supply by a DC power supply can be realized.

[0034] According to a preferred embodiment of the present invention, in the circuit part, the power supply interface can also be used for data transmission.

[0035] In a second aspect, the present invention seeks to protect the application of the above-mentioned handheld card strip detection device in the field of card strip detection.

[0036] Beneficial effects of the present invention: The present invention provides a handheld card strip detection device for compatibility and portable detection of two types of card strips, namely immunofluorescence chromatography card strips and colloidal gold card strips. The handheld card strip detection device provided by the present invention has a compact internal layout and can be handheld and portable. The outer shell of the device is structurally set to a shape suitable for handholding, and is composed of an upper half of the outer shell and a lower half of the outer shell, forming a complete enclosed space to protect the internal components from external interference and provide a good hand-held experience. A display screen and a switch are provided on the outer surface of the outer shell of the device. The switch can be used to control the display screen or switch the type of card strip to be detected through the switch, and control the display of the test results on the display screen to realize human-computer interaction. During the detection, the card strip can be manually placed in the card slot, and the card slot can be slid and pushed into the card slot under the coordination of the mobile positioning part and accurately positioned to the position to be tested inside the device for detection. This introduction mechanism of the card strip to be tested is simple and easy to use, does not require a complex mechanical structure, and can ensure accurate and stable detection positioning. The device is provided with a moving positioning part. In order to ensure that the card strip moves accurately, stably and precisely under the drive of the card slot, a series of related structures are provided, such as: especially in the moving positioning part of the device, an encoder and a code disk are provided, the encoder is not only used for precise positioning, but also can be used to accurately record the position information of the card strip, to ensure the position accuracy of the sample during the detection process, and the code disk is convenient for later data processing; by providing an optical coupler and its fixing structure, the accuracy of the positioning and detection results is ensured; the card strip is placed in the card slot, and the card slot is on the linear guide along the track through the spring buckle female seat at the end of the linear guide and the spring buckle male head on the slider to accurately position the card strip, ensuring the accuracy of the sliding positioning of the card strip, and the cooperation between the linear guide and the slider further ensures the smoothness and stability of the sliding movement; the moving positioning part is also provided with a damping rack, a rotating shaft, a gear and other structures, among which the damping rack increases the damping coefficient of the slider, reduces the vibration amplitude and speed of the slider, improves stability and safety, and ensures the stability of signal acquisition.

[0037] The present invention introduces an encoder in the mobile positioning part to replace the traditional time judgment method, and is equipped with a code disk structure, which can effectively solve the shortcomings of the time judgment method when detecting the immunofluorescence chromatography card strip, and avoid the adverse effects of the change in the advancement speed. Specifically, even if the advancement speed of the card slot changes, the encoder can monitor the change in the advancement position of the card slot in real time and generate a pulse signal, that is, the real-time position of the card slot can still be accurately recorded by the encoder, ensuring the position accuracy of the card strip during the detection process, and then ensuring that the distance between the T line (detection line) and the C line (control line) on the card strip remains consistent, avoiding the fluorescence curve offset caused by the change in advancement speed, and significantly improving the accuracy, stability and reliability of the detection results. In addition, since the encoder can provide high-precision position feedback and is equipped with a code disk, the code disk and the gear are installed on the rotating shaft, the rotation angle and position of the code disk are accurately measured, and the actual position of the card slot is determined by counting the number and direction of the pulse signal, and the moving physical signal is converted into an electrical signal, which is convenient for subsequent specific data processing.

[0038] The present invention provides a linear guide rail in the moving positioning part. At the same time, the slider is installed on the linear guide rail, the slot is fixed on the slider, and two damping racks are provided. One damping rack is fixed on the damping fixed block, and the other damping rack is fixed on the optical module fixed block. The damping fixed block is further fixed on the bottom of the shell. These optimized structural layouts ensure the coordinated operation of various components of the device and improve the overall stability and reliability.

[0039] The photoelectric detection part of the present invention is responsible for capturing the fluorescent signal on the immunofluorescence chromatography card strip or the color change on the colloidal gold card strip, thereby outputting quantitative or qualitative detection results. The photoelectric detection part is provided with an optical module shielding shell, which is used to shield external signal interference and ensure the stability of the photoelectric sensor. The optical module is installed in the optical module shielding shell, and the optical module shielding shell is fixed on the optical module fixing block. The optical module fixing block is composed of the upper part of the optical module fixing block and the lower part of the optical module fixing block. The lower part of the optical module fixing block is fixed to the bottom of the lower half of the shell. The upper part of the optical module fixing block and the lower part of the optical module fixing block are fixedly connected to form an "L" shape rotated 90 degrees clockwise. Such a fixed setting not only ensures that the optical module is located at the upper part of the card strip, but also ensures the effective protection and stable installation of the optical module. The light source fixing groove is used to fix the light source, the light source is used to emit an excitation light signal, and the photoelectric sensor fixing groove is used to fix the photoelectric sensor, and the photoelectric sensor is used to receive the photoelectric collection signal. The optical module of the present invention is installed in the optical module shielding shell, the optical module shielding shell is fixed in the optical module fixing block, and the optical module fixing block is fixed to the bottom of the shell. Such a layer-by-layer fixed and protected structure ensures effective protection and stable installation of the optical module. It is preferred that a layer of copper foil is attached to the outer surface of the optical module shielding shell to double the shielding of the external environment. The power supply current of the light source is controlled by the circuit part in the optical module. The higher the current, the brighter the light source. When the brightness of the light source is too high, the excessive light will be irradiated onto the card strip. If the immunofluorescence chromatography card strip is detected, the fluorescent signal emitted by the fluorescent substance will reach saturation or near saturation, which will reduce the amplitude of the change in the fluorescent signal value. This is equivalent to excessive brightness, which is conducive to reducing the influence of external conditions (such as concentration, temperature, etc.) on the fluorescent signal on the card strip and improving the accuracy of detection. In addition, compared with the method of adjusting the amplification factor of the circuit that relies on complex circuit design and debugging, the method of adjusting the current is simpler, more intuitive and easier to implement.

[0040] The handheld card strip detection device provided by the present invention is provided with a slidable sliding cover on the outer surface of the shell of the device, and a replaceable optical module carrying light sources of different wavelengths is matched, so that the user can easily replace the optical module, which can not only achieve compatibility with the two types of card strips of immunofluorescence chromatography card strips and colloidal gold card strips, but also expand the universal detection of immunofluorescence chromatography card strips marked with different fluorescent substances. The outer surface of the upper part of the shell of the present invention is provided with a slidable sliding cover, and the optical module is replaceable. The sliding cover and the optical module cooperate with each other, and different types of optical modules carrying different light sources are provided to meet the needs of different card strips to adapt to the type of light source and replacement, so that it can not only meet the detection needs of a variety of immunofluorescence chromatography card strips, but also solve the essential difficulties such as complex equipment structure and excessive volume caused by integrating a multi-light source system on a fluorescence detector.

[0041] In the circuit part of the present invention, the main control circuit can preferably use a high-speed single-chip microcomputer for core control processing, and the output data is matched with the software set up based on the filtering algorithm of Fourier transform to optimize the data processing process, speed up the processing speed of the detection data, and improve the accuracy, stability and reliability of the detection results. Among them, the Fourier filtering algorithm is based on the principle of Fourier transform, which can decompose the original signal into a series of frequency components, retain the useful signal by filtering out the noise components, thereby achieving signal purification. Specifically, the Fourier transform converts the signal in the time domain to the frequency domain, allowing the identification and removal of noise frequency components that do not belong to the real signal. By selecting appropriate filtering parameters, noise interference can be effectively reduced, and the purity and detection accuracy of the signal can be improved.

[0042] The present invention provides a handheld card strip detection device, which can simultaneously detect two kinds of card strips, namely, immunofluorescence chromatography card strips and colloidal gold card strips, and includes a compact, hand-held housing, which is small in size and convenient to carry; the housing of the device integrates three main parts, namely, a mobile positioning part, a photoelectric detection part, and a circuit part, thereby overcoming the technical problems of the large size of the device, high cost, and reliance on naked eye judgment in the traditional card strip detection method. The user only needs to manually push the immunofluorescence chromatography card strip or the colloidal gold card strip into the device, and the detection can be completed by the coordinated work of the mobile positioning part and the photoelectric detection part, and the detection speed and accuracy can be improved, and the detection cost can be reduced. In addition, the circuit part provides two power supply modes, and can also intuitively display the detection results on the display screen, so as to obtain accurate card strip detection results in real time, and can meet the needs of compatibility detection of multiple card strips in clinical scenarios. Therefore, the handheld card strip detection device provided by the present invention has the characteristics of small size, low cost, simple operation, short detection time, flexible use, high detection accuracy, etc., and is particularly suitable for promotion and application in POCT fields such as home and on-site. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 It is a three-dimensional diagram of the left front side of the handheld card strip detection device of the present invention; Figure 2 It is a top view of the handheld card strip detection device of the present invention; Figure 3 It is a rear view of the bottom of the handheld card strip detection device of the present invention; Figure 4 It is a three-dimensional diagram of the internal structure of the lower half of the housing of the handheld card strip detection device of the present invention; Figure 5 It is a top view of the internal structure of the lower half of the housing of the handheld card strip detection device of the present invention; Figure 6 It is a three-dimensional diagram of the left front side of the moving positioning part of the handheld card strip detection device of the present invention; Figure 7 It is a right front stereoscopic view of the moving positioning part of the handheld card strip detection device of the present invention; Figure 8 For Figure 7 A right front stereoscopic view of the foundation after removing the rotating bearing seat fixing block; Fig. 9 It is a magnified stereoscopic view of the local structure of the moving positioning part of the handheld card strip detection device of the present invention; Fig.10 It is a three-dimensional view and a top view of the linear guide rail related structures in the moving positioning part of the handheld card strip detection device of the present invention; Fig.11 It is a stereoscopic diagram of the photoelectric detection part of the handheld card strip detection device of the present invention; Fig.12 It is a stereoscopic diagram of an optical module in the photoelectric detection part of the handheld card strip detection device of the present invention; Fig.13 It is a cross-sectional view and a rear view of an optical module in the photoelectric detection part of the handheld card strip detection device of the present invention; Fig.14 This is an operation flow chart of replacing the optical module of the handheld card strip detection device of the present invention; Fig.15 The diagrams are the test results of the weak positive card strip, wherein A is the test result diagram of the handheld card strip detection device of the present invention, and B is the test result diagram of the motor scanning fluorescence instrument.

[0044] In the figure: 1-display screen; 2-upper part of the shell; 3-switch; 4-lower part of the shell; 5-card strip; 6-card slot; 7-slide cover; 8-power switch; 9-dual-color indicator light hole; 10-power interface; 11-optical coupler fixing block fixing hole; 12-optical module fixing block fixing hole; 13-encoder fixing block fixing hole; 14-optical module shielding shell; 15-main control board; 16-optical module; 17-battery; 18-power supply board; 19-optical coupler fixing block; 20-rotating shaft; 21-code disk; 22-encoder; 23-linear guide; 24-spring buckle mother seat; 25-dual-color indicator light; 26-optical coupler; 27-rotating bearing seat fixing block; 28-slider; 2 9-damping rack; 30-left-turn bearing seat; 31-right-turn bearing seat; 32-gear; 33-spring buckle male head; 34-lower part of optical module fixing block; 35-upper part of optical module fixing block; 36-photoelectric sensor fixing slot; 37-light source fixing slot; 38-transmitting lens; 39-optical module fixing hole; 40-receiving lens; 41-light exit hole; 42-light entrance hole; 43-metal slit; 44-filter; 45-wide-diameter filter; 46-left half of optical module; 47-right half of optical module; 48-immunofluorescence chromatography card strip optical module; 49-colloidal gold card strip optical module; 50-damping rack fixing block; 51-encoder fixing block. DETAILED DESCRIPTION

[0045] The embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In order to clearly and completely illustrate the specific exemplary embodiments of the handheld card strip detection device of the present invention, many specific details are described to fully improve and understand the overall inventive concept of the present invention. However, in other cases, one or more well-known embodiments may also be implemented without these specific details.

[0046] The present invention claims protection for a handheld card strip detection device. In the present invention, "detection" specifically refers to the detection of the results of antigen or antibody-specific immune reactions occurring on immunofluorescence chromatography card strips and colloidal gold card strips, and belongs to the field of in vitro diagnostic reagents. The handheld card strip detection device of the present invention is a matching detection instrument or device for two types of card strips, namely, immunofluorescence chromatography card strips and colloidal gold card strips. The fluorometer selected for the detection application comparison is a motor scanning fluorometer, which is a self-developed product of the laboratory of the applicant of the present invention.

[0047] According to the general inventive concept, the present invention provides a handheld card strip detection device, including a compact, hand-held housing, which is small and easy to carry; the housing of the device integrates three main parts, namely, a mobile positioning part, a photoelectric detection part, and a circuit part, which overcomes the technical problems of the large size of the device, high cost, and reliance on naked eye judgment in the traditional card strip detection method. The user only needs to manually push the immunofluorescence chromatography card strip or the colloidal gold card strip into the device, and the detection can be completed with the help of the coordinated work of the mobile positioning part and the photoelectric detection part, and the detection speed and accuracy can be improved, and the detection cost can be reduced. In addition, the circuit part provides two power supply modes, and can also intuitively display the test results on the display screen, so as to obtain accurate card strip test results in real time, which can meet the needs of compatibility detection of multiple card strips in clinical scenarios. In addition, the handheld card strip detection device provided by the present invention realizes the universal detection of immunofluorescence chromatography card strips marked with different fluorescent substances by providing a slidable slide cover on the outer surface of the housing of the device, and cooperating with a replaceable optical module carrying light sources of different wavelengths.

[0048] The following is combined with Figure 1-15 The structure and function of the handheld card strip detection device of the present invention are further described in detail.

[0049] Embodiment 1 Handheld card strip detection device The handheld card strip detection device of the present invention can simultaneously perform compatibility and portable detection on two types of card strips, namely, immunofluorescence chromatography card strips and colloidal gold card strips. The outer shell of the device is set to a shape suitable for handholding, and is composed of an upper shell part 2 and a lower shell part 4. The functional components arranged on the outer surface and inside of the outer shell of the device include a mobile positioning part, a photoelectric detection part and a circuit part. A display screen 1, a switch 3 and a slidable slide cover 7 for realizing human-computer interaction are also arranged on the outer surface of the outer shell of the device; the mobile positioning part is used to control the card strip 5 to be smoothly pushed into the device for detection; the photoelectric detection part is used to emit light and detect the collected light signal on the card strip 5; the circuit part is used to provide power supply and data transmission for the device; the mobile positioning part includes a card slot 6 for placing the card strip 5, a linear guide rail 23 and a slider 28 for providing a fixed sliding track, and an encoder 22 for ensuring stable sliding movement and precise alignment; the photoelectric detection part The detection part is used to detect the optical signal on the card strip 5, including an optical module 16, an optical module shielding shell 14 and an optical module fixing block. The optical module 16 is a replaceable structure. The optical module 16 is installed in the optical module shielding shell 14, and the optical module shielding shell 14 is fixed on the optical module fixing block; two optical paths are formed in the optical module 16, namely a transmitting optical path and a receiving optical path. The light source is arranged in the light source fixing groove 37, and the photoelectric sensor is arranged in the photoelectric sensor fixing groove 36. The transmitting optical path is used to irradiate the light emitted by the light source from the light source fixing groove 37 to the card strip 5, and the receiving optical path is used to emit the optical signal on the card strip 5 from the light exit hole 41 to the photoelectric sensor fixing groove 36, and the optical signal is collected by the photoelectric sensor fixed in the photoelectric sensor fixing groove 36 for detection; the circuit part includes a main control board 15, a battery 17, a power supply board 18 and a power interface 10 that can realize a dual power supply mode, wherein the power interface 10 can also be used for data transmission.

[0050] Figure 1 This is a three-dimensional diagram of the left front side of the handheld card strip detection device of the present invention. It can be seen that the overall shell of the device is composed of the upper shell part 2 and the lower shell part 4. These two parts can be tightly fastened together by various detachable methods such as buckles, stoppers and buckles, screws, elastic buckles or slide rails and slide grooves, which is convenient for later disassembly, replacement or maintenance; the material of the shell can be selected from resin, plastic, aluminum alloy or carbon fiber. Considering the cost, the material is preferably plastic. The color of the device shell is preferably black to minimize the interference of external light sources on the detection results.

[0051] Among them, the upper part of the upper part 2 of the shell is the front of the device, including a display screen 1, a switch 3 and a slidable slide cover 7; the display screen 1 can be an OLED screen, an LCD screen or a touch screen; the switch 3 is preferably a multi-directional switch, which is used to control the rapid switching of the detection of the two card strips, the immunofluorescence chromatography card strip and the colloidal gold card strip, and control the display of the detection results on the display screen 1; the display screen 1 and the switch 3 are both fixed on the front of the shell, facing the operator, which is convenient for rapid operation and viewing the detection results; the slide cover 7 can be slidably removed and can be used to replace the optical module. The lower part 4 of the shell is provided with a push-pull sliding slot 6, and the card strip 5 to be tested can be positioned and placed therein after the card slot 6 slides out. After the card strip 5 is placed, the card slot 6 can be manually pushed to slide into the inside of the device, and the card strip 5 can be tested; the card slot 6 is provided with a universal card strip insertion track that can smoothly place the two card strips, the immunofluorescence chromatography card strip and the colloidal gold card strip, to ensure that different card strips can be smoothly placed and slid by the card slot 6 to the detection position. When starting the test, the specific type or model of the card strip 5 to be tested can be selected through the switch 3, whether it is an immunofluorescence chromatography card strip or a colloidal gold card strip. By selecting the correct type or model of the card strip, the circuit part of the device can automatically adjust the detection parameters to ensure the accuracy and reliability of the test results.

[0052] Figure 2 This is a top view of the handheld card strip detection device of the present invention, that is, an appearance view of the top side of the device. Figure 2 It can be seen that a power switch 8, a two-color indicator light hole 9 and a power interface 10 are provided on the lower half of the housing 4, wherein the power switch 8 is used to control the on / off of the power supply of the entire device. Figure 5 From the perspective of the device, the dual-color indicator light hole 9 cooperates with the dual-color indicator light 25 to indicate the charging and discharging status of the built-in battery module to ensure that the device has power available at any time. The power interface 10 is used to charge the battery module or directly power the device, preferably a USB connector, such as the universal Type-C interface.

[0053] Figure 3 The structure layout of the handheld card strip detection device of the present invention is shown after the rear view of the bottom is rotated 90 degrees clockwise. The bottom of the device corresponds to the outer side of the bottom of the lower half 4 of the shell, and mainly has three fixing hole structures, which correspond to the optical coupling fixing block fixing hole 11, the optical module fixing block fixing hole 12 and the encoder fixing block fixing hole 13. Figure 4 See, the optical coupler fixing block fixing hole 11 is used to fix the optical coupler fixing block 19; Fig.11 The optical module fixing block fixing hole 12 is used to fix the optical module fixing block lower part 34, combined with Figure 8The encoder fixing block fixing hole 13 is used to fix the encoder fixing block 51; these three fixing holes can be preferably fixed continuously by screws. The encoder 22 can be a photoelectric encoder, an incremental encoder, a magnetic encoder, etc., and the present invention preferably uses an incremental encoder.

[0054] Figure 4 This is a three-dimensional diagram of the internal structure of the lower half of the housing of the handheld card strip detection device of the present invention (right front side), which shows the main functional components of the device, including the moving positioning part, the photoelectric detection part, and the circuit part. These three main functional components are fixedly arranged on the lower half 4 of the housing, wherein the moving positioning part drives the card strip 5 to move and position the detection under the photoelectric detection part. Figure 8-Figure 9It can be seen that the movable positioning part includes a rotating shaft 20, a code disk 21 and an encoder 22, etc. The code disk 21 is installed on the rotating shaft 20 to ensure the stable connection and precise alignment of the relevant components; with the help of the coordinated work of the encoder 22 and the linear guide rail 23, the card strip 5 placed on the card slot 6 is manually pushed and slid and positioned to the inside of the device for detection, and the encoder 22 is fixed in the encoder fixing block 51, and the encoder fixing block 51 is further fixed to the bottom of the lower half of the shell 4 through the encoder fixing block fixing hole 13; it is used to ensure the stability of the connection and the precise control of the alignment, thereby ensuring the stable operation and accurate detection of the device. The photoelectric detection part mainly includes an optical module shielding shell 14, an optical module 16, an optical coupler fixing block 19, etc. The optical module 16 is fixed in the optical module shielding shell 14, and the optical module shielding shell 14 is fixed above the upper part 35 of the optical module fixing block, which is used to shield external signal interference and ensure the stability of the photoelectric sensor. The optical coupler fixing block 19 is fixed on the right side of the device and is used to fix three optical couplers 26; the circuit part integrates the functions of the power supply circuit and the main control circuit, provides a stable power supply for the entire system, and is responsible for controlling the operation logic of the entire device, processing the data collected by the photoelectric detection part, and outputting the results, integrating a microcontroller, a power management chip, a memory and other necessary electronic components for data processing and result display. The specific components of the circuit part include a main control board 15, a battery 17, a power supply board 18, etc., which are respectively fixed on the left side of the lower half 4 of the housing, wherein the battery 17 is a rechargeable battery, and the power interface 10 can charge the battery 17; the battery 17 can be a lithium battery, a solar cell or a dry cell, preferably a lithium battery. The battery 17 and the power interface 10 cooperate to realize a dual power supply mode of battery 17 power supply or direct power supply of a DC power supply. The circuit part can also include a dual power supply switching circuit, a power supply circuit, a main control circuit, a photoelectric detection circuit, an amplification circuit and a constant current drive circuit from a functional point of view. Different circuit boards are interconnected through lines, preferably fixedly connected by screws, to ensure the stability and reliability of the entire system. Among them, the main control circuit can intelligently switch the detection mode, and can automatically adjust the detection parameters according to the type of card strip placed. Through the automatic identification and switching control of the main control circuit, it can not only be compatible with different types of card strips, but also ensure the intelligence, automation, accuracy and reliability of the detection results. The photoelectric detection circuit can automatically detect the information such as the stable connection status and position of all components, thereby ensuring the stable operation and accurate detection of the device. The main control circuit can preferably use a high-speed single-chip microcomputer for core control processing, and the output data cooperates with the software set up by the filtering algorithm based on Fourier transform to optimize the data processing process, speed up the processing speed of the detection data, and improve the accuracy, stability and reliability of the detection results. Figure 4 The connections between the related components are preferably fixed by screws. The encoder 22 can be a photoelectric encoder, a magnetic encoder or a mechanical encoder.

[0055] Figure 5 The upper housing part 2 and the optical module 16 of the handheld card strip detection device are first removed, and then the top view of the internal structure of the lower housing part 4 is rotated 90 degrees clockwise. Figure 4 It can be seen that: there are multiple optical couplers 26, preferably 3 optical couplers 26 are set, and the optical coupler 26 is fixedly installed on the optical coupler fixing block 19. On the one hand, the fixed installation position of the optical coupler 26 needs to be accurately positioned to ensure the accuracy of the detection. On the other hand, the optical coupler fixing block 19 has precise dimensions and an optical coupler fixing block fixing hole 11, which is used to fix and support the optical coupler 26. It can not only simplify the installation and improve the installation accuracy, but also further ensure that the optical coupler 26 can be accurately installed and positioned according to the predetermined position and angle, which is conducive to providing a stable and reliable detection signal, and further improves the accuracy of the detection result. The connection between the optical coupler related components (optical coupler 26, optical coupler fixing block 19 and optical coupler fixing block fixing hole 11) is preferably fixed by screws. Fig.10 It is a schematic diagram of the connection relationship of the linear guide rail 23 in the mobile positioning part. Figure 5 and Fig.10 Combined: the spring buckle female seat 24 is fixed at the end of the linear guide rail 23, and the corresponding spring buckle male head 33 is installed at the position of the slider 28 corresponding to the spring buckle female seat 24. When the slider 28 is pushed to the end, the spring buckle female seat 24 is engaged with the spring buckle male head 33 to ensure the accuracy of the sliding position of the slider 28, thereby ensuring that the card strip 5 is carried and slid to the correct position. In addition, the card slot 6 is fixed on the slider 28, and the slider 28 is installed on the linear guide rail 23, and ensures that the slider 28 slides on the linear guide rail 23. Pushing and pulling the card slot 6 can make the slider 28 slide forward or backward on the linear guide rail 23 smoothly. The linear guide rail 23 is further fixed at the bottom of the lower half 4 of the shell. The synergy between these components ensures the smoothness and stability of the sliding. The purpose of setting the relevant structure of the linear guide rail 23 is to provide a fixed sliding track for sliding movement, which can theoretically be replaced by a ball screw structure or a rack and pinion combination structure and other related movement forms.

[0056] Figure 6 This is a three-dimensional diagram of the left front side of the structure related to the moving positioning part of the handheld card strip detection device of the present invention. Figure 7 This is a right front side stereogram of the handheld card strip detection device of the present invention, excluding the structure of the movable positioning part of the optical coupling related components (optical coupling 26 and optical coupling fixing block 19, etc.). Figure 8 For Figure 7 The right front side stereogram of the structure of the movable positioning part after removing the shielding of the rotating bearing seat fixing block 27, Figure 6 to Figure 8 The flexibility and accuracy of the mobile positioning part of the handheld card strip detection device of the present invention are shown in detail in: Fig.10The present invention provides two types of card strips 5, namely, immunofluorescence chromatography card strips and colloidal gold card strips, which can be universally inserted into the track card slot 6. The card slot 6 drives the card strip 5 to slide smoothly on the linear guide rail 23 and reach the designated detection position; Figure 5 As can be seen, the linear guide rail 23 is fixed to the bottom of the lower half 4 of the housing, and a slot 6 is installed on the upper part of the slider 28. The slider 28 slides along the track on the linear guide rail 23 and drives the slot 6 installed thereon to slide along the track. Figure 7-Figure 8 As can be seen, the mobile positioning part is also provided with two damping racks 29, two damping rack fixing blocks 50, two gears 32, two rotating bearing seats (left rotating bearing seat 30, right rotating bearing seat 31) and rotating bearing seat fixing block 27 in a symmetrical layout. The two damping rack fixing blocks 50 are fixed on the side wall of the lower half 4 of the housing, and the two damping racks 29 are respectively fixed on the two damping rack fixing blocks 50. The damping rack 29 increases the damping coefficient of the slider 28 to ensure the signal To ensure the stability of the collection, the damping rack 29 is in contact with the gear 32 teeth, both ends of the rotating shaft 20 pass through and are positioned in the two rotating bearing seats, and at the same time, the two rotating bearing seats (left rotating bearing seat 30 and right rotating bearing seat 31) are connected through the rotating shaft 20, and the rotating bearing seat fixing block 27 is located on the outside of the left rotating bearing seat 30, and the two rotating bearing seats are respectively fixed on the rotating bearing seat fixing block 27 and the optical coupling fixing block 19, and the rotating bearing seat fixing block 27 is further fixed to the bottom of the lower half 4 of the outer shell. Figure 8 It can also be seen that the encoder 22 is fixed on the encoder fixing block 51, and the encoder fixing block 51 is further fixed to the bottom of the lower half of the housing 4; Figure 8 and Fig. 9 In combination with the installation of the code disc 21 and the gear 32 on the rotating shaft 20, the structural layout of the movable positioning part ensures the coordinated operation between the various components and improves the overall stability and reliability.

[0057] Fig. 9 Specifically shows a three-dimensional diagram of the local structure of the mobile positioning part of the handheld card strip detection device of the present invention, combined with Figure 7-8 As described above, it can be further seen that the code disc 21 and the gear 32 are both mounted on the rotating shaft 20, and the rotating shaft 20 is fixed to two rotating bearing seats (right rotating bearing seat 31 and left rotating bearing seat 30); wherein, the right rotating bearing seat 31 is fixed on the optical coupler fixing block 19, the optical coupler 26 is also fixed on the optical coupler fixing block 19, and the left rotating bearing seat 30 is fixed on the rotating bearing seat fixing block 27. Such a structure ensures the stable connection and precise alignment of all components, thereby ensuring the stable operation and accurate detection of the device.

[0058] Fig.11 A stereoscopic diagram of the photoelectric detection part of the handheld card strip detection device of the present invention is shown. Fig.12 It is a stereoscopic diagram of the optical module in the photoelectric detection part. Fig.13Further cross-sectional view and rear view comparison of the optical module in the photoelectric detection part. Figure 11 to Figure 13 Combined with visible: Fig.11 It can be seen that the photoelectric detection part includes an optical module 16, an optical module shielding shell 14 and an optical module fixing block. The optical module 16 can adapt to two different types of card strips, namely immunofluorescence chromatography card strips and colloidal gold card strips, and can not only identify fluorescent signals but also detect color changes caused by colloidal gold particles; the optical module 16 is placed in the optical module shielding shell 14, and then the optical module shielding shell 14 is fixed on the optical module fixing block, wherein the optical module fixing block is composed of an optical module fixing block upper part 35 and an optical module fixing block lower part 34, and the optical module fixing block lower part 34 is fixed to the bottom of the lower half of the shell 4, and the optical module fixing block upper part 35 and the optical module fixing block lower part 34 are fixedly connected to form an "L" shape rotated 90 degrees clockwise, and then the optical module shielding shell 14 is fixed on the upper surface of the optical module fixing block upper part 35. The core purpose of setting the optical module shielding shell 14 and the optical module fixing block is to ensure effective protection and stable installation of the optical module 16. Fig.12 It can be seen that the optical module 16 is mainly composed of the left half 46 and the right half 47 of the optical module, the purpose of which is to facilitate the installation of two lenses (transmitting lens 38, receiving lens 40) and two filters (filter 44, wide-diameter filter 45), and these two parts are fixed through the optical module fixing hole 39. The optical module 16 is also provided with a photoelectric sensor fixing groove 36 and a light source fixing groove 37, wherein the photoelectric sensor fixing groove 36 is used to fix the photoelectric sensor responsible for receiving the photoelectric acquisition signal, such as a photodiode (PD), a photoresistor, a phototransistor, a photocoupler or a photocell, etc., preferably a photodiode with fast response and high sensitivity; the light source fixing groove 37 is used to install a light source, which can be a light source for stimulating the fluorescent substance on the immunofluorescence chromatography card strip to emit light, or a light source for identifying the color change on the colloidal gold card strip, or both light sources. Fig.13It can be seen from the rear view of the optical module 16 that the bottom of the optical module 16 includes a light entrance hole 42 for emitting light from the light source and a light exit hole 41 for receiving light emitted by the card strip 5. The cross-sectional view of the optical module 16 shows two light paths, namely, the light path for emitting light from the light source (referred to as the emitting light path) and the light path for collecting light (referred to as the receiving light path). Specifically, the path of the emitting light path includes the light source being emitted from the light source fixing groove 37, and then being emitted from the light entrance hole 42 after passing through the filter 44, the metal slit 43 and the emitting lens 38 in sequence. The number of the metal slits 43 and the emitting lenses 38 on the emitting light path is preferably set to 2, the purpose is to filter, gather and shape the emitted light, and preferably, the two emitting lenses 38 are set between the two metal slits 43. When the light source in the emitting light path is an LED light source, the LED light source is preferably a white LED and / or a green LED. If the light source is set to be a single white LED, the filter may not be configured. The light sheet 44, the white LED can more widely excite the fluorescent material and collect the emitted fluorescence. If the light source is set as a separate green LED, it can be matched with the filter 44. After the emitted light passes through the filter 44, it is filtered, focused and shaped by two metal slits 43 and the emission lens 38, and then the light spot formed from the light entrance hole 42 is irradiated onto the card strip 5, which is relatively more suitable for detecting colloidal gold card strips; the receiving light path is used to receive the light emitted by the card strip 5. After the light is emitted from the light exit hole 41, it passes through the receiving lens 40 and the wide-diameter filter 45 in sequence and enters the photoelectric sensor fixing groove 36, and is captured by the photoelectric sensor set in the photoelectric sensor fixing groove 36, wherein the filter 44 is preferably a square with a side length of 5 mm, and can be a green filter or a red filter. When the light source is a green LED, a green filter is preferred; the wide-diameter filter 45 is relatively wider than the filter 44, and is preferably a circle or semicircle with a diameter of 8 mm. In addition, Fig.13 The cross-sectional view of FIG. 1 also shows that an optical module fixing hole 39 is provided for fixing the optical module. Fig.12 The left half 46 and the right half 47 of the optical module are shown as being fixedly connected.

[0059] Fig.14The operation flow chart of replacing the optical module 16 of the handheld card strip detection device of the present invention is as described above. The light source of the present invention can be an LED light source, and the light source can also be a tunable laser. The wavelength of the light emitted by the tunable laser is switched by the circuit part to meet the excitation requirements of different fluorescent substances. In addition, according to the main detection requirements and the specific type of fluorescent substance on the card strip 5 to be tested, the light source can also be set to an ultraviolet light source (suitable for fluorescein fluorescent markers) or a laser light source (suitable for up-conversion nanoparticle fluorescent markers) suitable for a specific immunofluorescence chromatography card strip, etc. The flexibility of the handheld card strip detection device of the present invention is also reflected in that the optical module 16 can be configured to meet the detection requirements of the immunofluorescence chromatography card strip and the colloidal gold card strip according to different light source configurations. For example, the immunofluorescence chromatography card strip optical module 48 is an optical module 16 that can be more widely adapted to immunofluorescence chromatography card strips for different fluorescent markers, and is preferably configured as a white LED light source; the colloidal gold card strip optical module 49 is an optical module 16 that can maximize the recognition rate and accuracy of the color change caused by the aggregation state of the colloidal gold particles, and is preferably configured as an optical module 16 containing a green light source. Figure 1 It can be seen that since a slidable slide cover 7 is provided, and the optical module 16 is an installation structure that is easy to replace, such as a snap-on type, a magnetic type, a hook type, a slot type, etc., the slide cover 7 and the replaceable optical module 16 cooperate to provide a variety of different models of optical modules 16 according to specific needs to meet the requirements of different card strip adaptation types and easy replacement. This can not only adapt to the detection needs of various immunofluorescence chromatography card strips, but also solve the essential difficulties such as complex equipment structure and excessive volume caused by integrating a multi-light source system on a fluorescence detector.

[0060] The optical module 16 in the photoelectric detection part of the present invention is replaceable. The specific operation process of replacing the optical module 16 is as follows: generally, the slide cover 7 may be marked with an arrow indicating the direction of sliding to the opening. When replacement is required, the first step is to manually slide the slide cover 7 in the direction indicated by the arrow to expose the optical module 16 set in the optical module shielding shell 14; the second step is to remove the damaged or unsuitable optical module 16 inside according to the detection requirements, and the figure shows that the existing colloidal gold card strip optical module 49 is removed; the third step is to select a new or most suitable optical module 16, position and install it in the optical module shielding shell 14, and then manually slide the slide cover 7 back to the original position and buckle it. For example, the figure shows that the new or most suitable immunofluorescence chromatography card strip optical module 48 is replaced and inserted, and the replacement of the optical module 16 can be completed. The optical module 16 of the present invention is not only protected by the optical module shielding shell 14, but also further sealed and protected by the device housing, which not only ensures the normal operation of the device, but also improves the accuracy and stability of optical detection. The replaceable setting of the optical module 16 meets the diverse needs of users, and the replacement setting makes the operation simple and efficient, and meets the diverse detection tasks of two different card strips, namely, the immunofluorescence chromatography card strip and the colloidal gold card strip.

[0061] The present invention Fig.14 The replaceable operation of the optical module 16 is shown. In order to meet the needs of card strip detection diversity or further reduce costs, the light source in the optical module 16 can also be set to a replaceable structure. For example, different light sources that are compatible with detection or most suitable for immunofluorescence chromatography card strips and colloidal gold card strips are set to different models that are adapted to the size of the light source fixing groove 37. According to the detection requirements or the repair requirements of the damaged light source, only the light source needs to be replaced. In addition, the light source in the present invention is preferably an LED light source because: if the light source is a green LED, it has a specific wavelength range, can be used to excite fluorescent signals, and can provide more uniform lighting and enhance color contrast. It can not only be used for the detection of immunofluorescence chromatography card strips, but also can more accurately identify color changes on colloidal gold card strips. Therefore, it is more suitable for detecting colloidal gold card strips. However, if the excitation wavelength does not match, the green LED light source may not be able to effectively excite the fluorescent signal, and is not suitable for the detection needs of immunofluorescence chromatography card strips; if the light source is a white LED, since the radiation range of the white LED light source includes the wavelength that can excite fluorescent markers and the spectral range is wider, it can be widely used to excite immunofluorescence chromatography card strips whose fluorescent markers are quantum dots, and can also meet the detection needs of colloidal gold card strips with relatively low requirements for light sources.

[0062] Example 2 Detection Application According to the overall concept of the present invention, a handheld card strip detection device is provided, which is a portable device suitable for the field of POCT. On the premise of being able to perform compatibility detection on immunofluorescence chromatography card strips and colloidal gold card strips, the detection sensitivity of different card strips can be further improved. In the process of using the handheld card strip detection device of the present invention for detection, the peak ratio of T peak to C peak, negative and positive judgment and other related data processing, filtering and other related information can be completed and synchronized through the display screen 1 for intuitive feedback and presented to the user.

[0063] Compared with negative and strong positive card strips, weak positive card strips have low signal strength and are difficult to distinguish from background noise. If sensitive detection of weak positive card strips can be achieved, the detection sensitivity of the handheld card strip detection device of the present invention can be better reflected. Therefore, a weak positive immunofluorescence chromatography card strip was selected, and the handheld card strip detection device provided by the present invention and the motor scanning fluorescence instrument were used for detection and comparison. The detection results are as follows: Fig.15 As shown, after the dynamic data of the detection result of the handheld card strip detection device of the present invention is output to the intelligent terminal (such as a computer) through the power interface 10, the dynamic curve obtained by fitting the data is as shown in FIG. Fig.15 As shown in A, the curve has an obvious peak value, and the peak ratio is 0.22, which can be judged as positive. Fig.15 As shown in B, the peak ratio is 0.25) by comparison, it can be seen that the sensitivity, accuracy and reliability of the detection results of the handheld card strip detection device of the present invention are comparable to those of ordinary fluorescence detectors, and it can distinguish negative, weak positive and positive card strips in a portable, intuitive and accurate manner.

[0064] The present invention is a portable handheld device used for compatibility detection of two types of card strips, namely, immunofluorescence chromatography card strips and colloidal gold card strips. With its compact appearance, simplified structure and avoidance of expensive consumables, the cost is reduced, the technical problems of large size and high cost of traditional equipment are solved, and the simplicity, flexibility and convenience of the operation process are ensured. After the card strip is inserted, the automated detection process can be realized, which can not only avoid the risk of misjudgment due to the result interpretation being susceptible to ambient light and personal subjective factors, but also improve the automation and accuracy of the detection, and greatly promote its popularization and application in the POCT field.

[0065] The specific embodiments of the present invention have been described in detail so that those skilled in the art can easily understand them. However, the above specific embodiments are only used to specifically illustrate the technical solutions or preferred solutions of the present invention in conjunction with the drawings, rather than to limit the scope of protection of the present invention. According to all the descriptions disclosed, the details can be appropriately modified or replaced without departing from the essence and scope of the technical solutions of the present invention. The full scope of the present invention is given by the attached claims and any equivalents thereof.

Claims

1. A handheld card strip detection device, characterized in that: Used for compatibility and portable detection of multiple card strips (5), the shell of the device is arranged in a shape suitable for hand-holding, and is composed of an upper shell part (2) and a lower shell part (4), and the functional components arranged on the outer surface and inside of the shell of the device include a mobile positioning part, a photoelectric detection part and a circuit part, and a display screen (1) and a switch (3) for realizing human-computer interaction are also arranged on the outer surface of the shell of the device; the mobile positioning part is used to control the card strip (5) to be smoothly pushed into the inside of the device for detection; the photoelectric detection part is used to emit light and detect the collected light signal on the card strip (5); and the circuit part is used to provide power supply and data transmission for the device; The movable positioning part comprises a card slot (6) for placing a card strip (5), a linear guide rail (23) and a slider (28) for providing a fixed sliding track, and an encoder (22) for ensuring stable sliding movement and accurate alignment; The photoelectric detection part is used to detect the optical signal on the card strip (5), and comprises an optical module (16), an optical module shielding shell (14) and an optical module fixing block, wherein the optical module (16) is installed in the optical module shielding shell (14), and the optical module shielding shell (14) is fixed on the optical module fixing block; two optical paths are formed in the optical module (16), namely an emission optical path and a receiving optical path, the light source is arranged in the light source fixing groove (37), and the photoelectric sensor is arranged in the photoelectric sensor fixing groove (36); the emission optical path is used to irradiate the light emitted by the light source from the light source fixing groove (37) onto the card strip (5), and the receiving optical path is used to emit the optical signal on the card strip (5) from the light exit hole (41) to the photoelectric sensor fixing groove (36), and the optical signal is collected by the photoelectric sensor fixed in the photoelectric sensor fixing groove (36) for detection; The circuit part comprises a main control board (15), a battery (17), a power supply board (18) and a power supply interface (10).

2. The handheld card strip detection device according to claim 1, characterized in that: The mobile positioning part also includes an optical coupler (26), a slider (28), a damping rack (29), a rotating shaft (20), a code disc (21), a gear (32) and related fixed structures for providing precise control and smooth movement for the linear guide rail (23). The optical coupler (26) is used to ensure the accuracy of signal transmission. The slider (28) is used to ensure the smoothness and stability of the movement of the linear guide rail (23). The damping rack (29) increases the damping coefficient of the slider (28) to ensure the stability of signal acquisition. The coordinated cooperation of the rotating shaft (20), the code disc (21) and the gear (32) ensures the stable connection and precise alignment of the moving components.

3. The handheld card strip detection device according to claim 1, characterized in that: In the photoelectric detection part, the optical module fixing block comprises an optical module fixing block upper part (35) and an optical module fixing block lower part (34), the optical module fixing block upper part (35) is used to fix the optical module shielding shell (14), and the optical module fixing block lower part (34) is fixed to the bottom of the lower half of the housing (4), and the optical module fixing block upper part (35) and the optical module fixing block lower part (34) are fixedly connected.

4. The handheld card strip detection device according to claim 1, characterized in that: The emission light path is formed by a light source, a light source fixing groove (37), a filter (44), a metal slit (43), an emission lens (38) and a light entrance hole (42); and the receiving light path is formed by a light exit hole (41), a receiving lens (40), a wide-diameter filter (45), a photoelectric sensor fixing groove (36) and a photoelectric sensor.

5. The handheld card strip detection device according to claim 1, characterized in that: The light source is an LED light source, and the photoelectric sensor is a photodiode.

6. The handheld card strip detection device according to claim 1, characterized in that: The outer surface of the upper half (2) of the housing is provided with a slidable sliding cover (7), and the optical module (16) is a replaceable structure.

7. The handheld card strip detection device according to any one of claims 1 to 6, characterized in that: The power supply current of the light source is controlled by the circuit part.

8. The handheld card strip detection device according to claim 2, characterized in that: The fixing structure comprises an optical coupler fixing block (19), a right-turn bearing seat (31), a left-turn bearing seat (30), a rotating bearing seat fixing block (27), a damping rack fixing block (50), and an encoder fixing block (51), wherein the optical coupler fixing block (19) is used to fix the optical coupler (26); the code disk (21) and the gear (32) are mounted on the rotating shaft (20); the right-turn bearing seat (31) and the left-turn bearing seat (30) are used to fix the rotating shaft (20); the optical coupler fixing block (19) and the rotating bearing seat fixing block (27) respectively fix the right-turn bearing seat (31) and the left-turn bearing seat (30); the damping rack fixing block (50) is used to fix the damping rack (29); and the encoder fixing block (51) is used to fix the encoder (22).

9. The handheld card strip detection device according to claim 4, characterized in that: The number of the metal slits (43) and the number of the emitting lenses (38) are both set to 1-3.

10. Application of the handheld card strip detection device described in any one of claims 1 to 9 in card strip detection.

Images