Fluorescence emission intensity acquisition method based on enhanced fluorescence data set, electronic equipment and medium

Abstract

The invention discloses a fluorescence emission intensity acquisition method based on an enhanced fluorescence data set, electronic equipment and a medium. The method comprises the following steps: carrying out fluorescence detection on fluorescent molecules; under the illumination of exciting light with fixed intensity, recording a fluorescence image of fluorescent molecules through a fluorescence camera, recording illumination light power through a power meter, obtaining reference fluorescence emission power, and recording illumination light wavelength and fluorescence emission wavelength through a spectrograph; recording the position of the fluorescent molecule, the concentration of the fluorescent molecule, the position of the fluorescent camera, the position of the power meter and the position of the spectrograph so as to obtain a fluorescent data set serving as a fluorescent reference; extending the depth of the fluorescent molecules, the scattering coefficient, the absorption coefficient and / or the wavelength of the illumination light to obtain illumination light change and fluorescence change so as to enhance a fluorescence data set; and multiplying the reference fluorescence emission intensity with the illumination light change and the fluorescence change to obtain the final fluorescence emission intensity.

Description

Technical Field

[0001] The present invention relates to the technical field of biological fluorescence imaging, and in particular to a method, electronic equipment and medium for acquiring fluorescence emission intensity based on enhanced fluorescence data set. Background Art

[0002] Medical optics plays an important role in clinical diagnosis and treatment. Optical imaging instruments and equipment represented by endoscopes can effectively identify tissue lesions, improve the accuracy of diagnosis, and reduce the risk of treatment. However, optical imaging equipment is a precision instrument with high system complexity. In the long-term use process, if there is a lack of appropriate verification and calibration methods, the performance of the equipment will not be effectively guaranteed; endoscopes with unqualified quality not only cannot promote clinical diagnosis and treatment, but also have the risk of causing medical hazards such as misjudgment. Therefore, medical optical imaging equipment requires reliable standards for effectiveness verification. Conventional standards such as resolution test plates lack fluorescence and are not suitable for performance verification of fluorescent imaging equipment. Physical phantoms with molecular luminescence and digital phantoms with light field-controlled luminescence provide good methods and means for performance verification of fluorescent imaging equipment. Digital phantoms can simulate the spectrum and light intensity of the target well, and become reliable standards for equipment verification. Since the data source of digital phantoms is optical imaging, when dealing with a variety of physical scenes, the sampling rate of optical imaging is limited, it is difficult to achieve extensive imaging, and the workload required is huge. Summary of the invention

[0003] The purpose of the present invention is to address the deficiencies of the prior art and provide a method, electronic device and medium for acquiring fluorescence emission intensity based on an enhanced fluorescence data set.

[0004] In a first aspect, an embodiment of the present invention provides a method for acquiring fluorescence emission intensity based on an enhanced fluorescence data set, the method comprising:

[0005] Fluorescence detection of fluorescent molecules; under the illumination of fixed intensity excitation light, the fluorescence image of the fluorescent molecules is recorded by a fluorescence camera, the illumination light power and the fluorescence emission power are recorded by a power meter, and the illumination light wavelength and the fluorescence emission wavelength are recorded by a spectrometer, thereby obtaining a fluorescence data set as a fluorescence benchmark;

[0006] Extending the depth of fluorescent molecules, scattering coefficients and / or absorption coefficients, illumination changes and fluorescence changes to enhance fluorescence data sets;

[0007] The final fluorescence emission intensity is obtained by multiplying the baseline fluorescence emission intensity with the illumination light power change and the fluorescence power change.

[0008] Furthermore, it also includes:

[0009] Record the position of fluorescent molecules, the concentration of fluorescent molecules, the position of the fluorescence camera, the position of the power meter, and the position of the spectrometer.

[0010] Furthermore, the process of obtaining the reference fluorescence emission power includes:

[0011] A simulated fluorescent light source is used, the light intensity of the simulated fluorescent light source is adjusted, and then a fluorescent camera and a power meter are used to detect and collect multiple sets of sample points of fluorescent emission power and fluorescent grayscale value, so as to obtain a mapping relationship between fluorescent emission power and fluorescent grayscale value;

[0012] The grayscale value of the fluorescence image is read, and the reference fluorescence emission power is obtained according to the mapping relationship between the fluorescence emission power and the fluorescence grayscale value.

[0013] Furthermore, the method further comprises: obtaining a reference fluorescence emission intensity, comprising:

[0014] A filter that can eliminate excitation light is placed in front of the power meter, so that the detection result of the power meter is not affected by the excitation light;

[0015] The fluorescence emission power is collected by a power meter, and the reference fluorescence emission intensity is calculated based on the fluorescence spectrum integral relationship.

[0016] Further, the depth of the fluorescent molecule, the scattering coefficient and / or the absorption coefficient are expanded to obtain the illumination light change and the fluorescence change including:

[0017] Set the fluorescence molecule depth z and scattering coefficient μ s and / or absorption coefficient μ a range, and the illumination light wavelength λ i , calculate the change of the illumination light after propagating through the depth, recorded as the illumination light power change δI;

[0018] Set the fluorescence molecule depth z and scattering coefficient μ s and / or absorption coefficient μ a The range of fluorescence emission wavelength λ f , calculate the change of fluorescence after propagation through this depth, recorded as the fluorescence power change δF;

[0019] Further, the depth range is extended to δz, the depth sampling rate is set to σz, a series of depth values ​​in the range of 0 to δz and with a resolution of σz are obtained, and the illumination light power change δI and the fluorescence power change δF corresponding to each depth value are calculated;

[0020] Expand the scattering range to δμ s , set the scattering sampling rate to σμ s , we get 0~δμ s interval and resolution is σμ sA series of scattering values ​​are obtained, and the illumination light power change δI and fluorescence power change δF corresponding to each scattering value are calculated;

[0021] or,

[0022] Extending the absorption range to δμ a , set the absorption sampling rate to σμ a , we get 0~δμ a interval and resolution is σμ a A series of absorption values ​​are obtained, and the illumination light power change δI and fluorescence power change δF corresponding to each absorption value are calculated.

[0023] Furthermore, the expression for the final fluorescence emission intensity obtained by multiplying the reference fluorescence emission intensity by the illumination light power change and the fluorescence power change is as follows:

[0024] P2=P1×δI×δF

[0025] Where P2 represents the final fluorescence emission intensity, P1 represents the reference fluorescence emission intensity, δI represents the illumination light power change, and δF represents the fluorescence power change.

[0026] In a second aspect, an embodiment of the present invention provides an electronic device, comprising a memory and a processor, wherein the memory is coupled to the processor; wherein the memory is used to store program data, and the processor is used to execute the program data to implement the above-mentioned method for acquiring fluorescence emission intensity based on an enhanced fluorescence data set.

[0027] In a third aspect, an embodiment of the present invention provides a computer-readable storage medium having a computer program stored thereon, which, when executed by a processor, implements the above-mentioned method for acquiring fluorescence emission intensity based on an enhanced fluorescence data set.

[0028] In a fourth aspect, an embodiment of the present invention provides a computer program product, including a computer program / instruction, which, when executed by a processor, implements the above-mentioned method for acquiring fluorescence emission intensity based on an enhanced fluorescence data set.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The present invention provides a method for acquiring fluorescence emission intensity based on an enhanced fluorescence data set. The present invention combines experimental acquisition and simulation calculation, not only providing accurate measurement of baseline fluorescence emission intensity, but also obtaining illumination light changes and fluorescence changes by expanding the depth of fluorescent molecules, scattering coefficients and / or absorption coefficients, so as to enhance the fluorescence data set and expand the parameter range of the fluorescence data set. The present invention can act on the fluorescence data set according to the change in fluorescence emission intensity and calculate the final fluorescence emission intensity. The present invention not only takes into account the luminescence characteristics of the fluorescent molecules themselves, but also takes into account the influence of biological tissues on the fluorescence signal, thereby realizing a comprehensive simulation of the fluorescence imaging process. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative labor.

[0032] Figure 1 is a flow chart of a method for acquiring fluorescence emission intensity based on an enhanced fluorescence data set provided by an embodiment of the present invention;

[0033] Figure 2 is a schematic diagram of obtaining a fluorescence data set provided by an embodiment of the present invention;

[0034] Figure 3 is a schematic diagram of calculating the final fluorescence emission intensity provided by an embodiment of the present invention;

[0035] Figure 4 It is a schematic diagram of an electronic device provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0036] Exemplary embodiments will be described in detail herein, examples of which are shown in the accompanying drawings. When the following description refers to the drawings, the same numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present invention. Instead, they are merely examples of devices and methods consistent with some aspects of the present invention as detailed in the appended claims.

[0037] The terms used in the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The singular forms "a", "the" and "the" used in the present invention and the appended claims are also intended to include plural forms unless the context clearly indicates other meanings. It should also be understood that the term "and / or" used herein refers to and includes any or all possible combinations of one or more associated listed items.

[0038] It should be understood that although the terms first, second, third, etc. may be used in the present invention to describe various information, these information should not be limited to these terms. These terms are only used to distinguish the same type of information from each other. For example, without departing from the scope of the present invention, the first information may also be referred to as the second information, and similarly, the second information may also be referred to as the first information. Depending on the context, the word "if" as used herein may be interpreted as "at the time of" or "when" or "in response to determining".

[0039] The present invention is described in detail below in conjunction with the accompanying drawings. In the absence of conflict, the features of the following embodiments and implementations can be combined with each other.

[0040] like Figure 1 As shown, an embodiment of the present invention provides a method for obtaining fluorescence emission intensity based on an enhanced fluorescence data set, the method comprising the following steps:

[0041] Step S1, performing fluorescence detection on the fluorescent molecules; including: placing the fluorescent molecule sample at a suitable position, recording the position X of the sample sample ; Place the fluorescence camera in a position where it can be imaged and record the position X of the fluorescence camera camera ; Turn on the excitation light, and record the fluorescence image of the fluorescent molecules through the fluorescence camera under the illumination of the excitation light of fixed intensity; Place the power meter and spectrometer at a position where the light signal can be detected, and also record the position of the power meter X power and the position X of the spectrometer spectra ; Record the illumination light power through the power meter and obtain the fluorescence emission power, record the illumination light wavelength and the fluorescence emission wavelength through the spectrometer, and record the fluorescent molecule position, fluorescent molecule concentration, fluorescence camera position, power meter position, and spectrometer position to obtain the fluorescence data set.

[0042] It should be noted that if Figure 2 As shown, the stored fluorescence data set contains multiple dimensions, and the records of multiple dimensions ensure the traceability of the fluorescence data set.

[0043] Furthermore, the process of obtaining the reference fluorescence emission power includes:

[0044] A simulated fluorescent light source is used, the light intensity of the simulated fluorescent light source is adjusted, and then a fluorescent camera and a power meter are used to detect and collect multiple sets of sample points of fluorescent emission power and fluorescent grayscale value, so as to obtain a mapping relationship between fluorescent emission power and fluorescent grayscale value;

[0045] The grayscale value of the fluorescence image is read, and the reference fluorescence emission power is obtained according to the mapping relationship between the fluorescence emission power and the fluorescence grayscale value.

[0046] Furthermore, the method further comprises: obtaining a reference fluorescence emission intensity, comprising:

[0047] A filter that can eliminate excitation light is placed in front of the power meter, so that the detection result of the power meter is not affected by the excitation light;

[0048] The fluorescence emission power is collected by a power meter, and the reference fluorescence emission intensity is calculated based on the fluorescence spectrum integral relationship.

[0049] Step S2, expanding the depth of fluorescent molecules, scattering coefficients and / or absorption coefficients, obtaining illumination light changes and fluorescence changes, so as to enhance the fluorescence data set.

[0050] Specifically, the step S2 includes:

[0051] Set the fluorescence molecule depth z and scattering coefficient μ s and / or absorption coefficient μ a range, and the illumination light wavelength λ i , calculate the change of the illumination light after propagating through the depth, recorded as the illumination light power change δI;

[0052] Set the fluorescence molecule depth z and scattering coefficient μ s and / or absorption coefficient μ a The range of fluorescence emission wavelength λ f , calculate the change of fluorescence after propagation through this depth, recorded as the fluorescence power change δF;

[0053] Furthermore, the expansion process may include:

[0054] The depth range is expanded to δz, the depth sampling rate is set to σz, a series of depth values ​​in the range of 0 to δz with a resolution of σz are obtained, and the illumination light power change δI and the fluorescence power change δF corresponding to each depth value are calculated;

[0055] Expand the scattering range to δμ s , set the scattering sampling rate to σμ s , we get 0~δμ s interval and resolution is σμ s A series of scattering values ​​are obtained, and the illumination light power change δI and fluorescence power change δF corresponding to each scattering value are calculated;

[0056] or,

[0057] Extending the absorption range to δμ a , set the absorption sampling rate to σμ a , we get 0~δμ a interval and resolution is σμ aA series of absorption values ​​are obtained, and the illumination light power change δI and fluorescence power change δF corresponding to each absorption value are calculated.

[0058] It should be noted that the present invention uses simulation to simulate the effect of parameter changes on fluorescence emission intensity. These parameters include but are not limited to the depth of biological tissue, scattering coefficient, absorption coefficient, etc., which are key factors affecting the propagation and detection of fluorescence signals. By calculating the changes in fluorescence emission intensity caused by these parameter changes, the present invention can expand the parameter range of the data set, thereby constructing a more comprehensive and diverse fluorescence phantom data set.

[0059] Step S3, multiplying the reference fluorescence emission intensity by the illumination light power change and the fluorescence power change to obtain a final fluorescence emission intensity.

[0060] like Figure 3 As shown, the expression is as follows:

[0061] P2=P1×δI×δF

[0062] Where P2 represents the final fluorescence emission intensity, P1 represents the reference fluorescence emission intensity, δI represents the illumination light power change, and δF represents the fluorescence power change.

[0063] It should be noted that the above calculation process can act on the fluorescence data set according to the change of fluorescence emission intensity to calculate the final fluorescence emission intensity. This step not only takes into account the luminescence characteristics of the fluorescent molecules themselves, but also takes into account the influence of biological tissues on the fluorescence signal, thereby achieving a comprehensive simulation of the fluorescence imaging process.

[0064] Example 1

[0065] Build a fluorescence collection system, which includes a precisely positioned fluorescence camera, a spectrometer, an optical power meter, and a sample holder; the fluorescence camera is used to record the two-dimensional spatial distribution of excitation light and fluorescence and the traceability reference, the spectrometer is used to record the excitation light wavelength and the fluorescence wavelength, and the optical power meter is used to record the excitation light power and the fluorescence power; the excitation light wavelength is selected to be 785nm;

[0066] Place the non-fluorescent sample on the sample stage, turn on the excitation light, use a spectrometer to detect the wavelength of the excitation light, and obtain a narrow band wavelength of 785nm; use a fluorescence camera to record the two-dimensional spatial distribution of the excitation light, and use a power meter to record the power of the excitation light at a position 2mm above the sample plane; set the light propagation depth to 2mm, and set the scattering coefficient of this layer to 10mm -1 , the absorption coefficient is 10cm -1, set the beam wavelength to 785nm, and use the two-dimensional spatial distribution of the excitation light recorded by the camera as the initial state of the simulation. After simulation, the power change amplitude of the excitation light after passing through a depth of 2mm is obtained.

[0067] Place the ICG fluorescent sample on the sample stage, turn on the excitation light, use a spectrometer to detect the fluorescence emission wavelength of the fluorescent sample, set 50nm equal intervals within the measured fluorescence bandwidth of 750-950nm, that is, 750nm, 800nm, 850nm, 900nm, 950nm, including 5 sample points, set the weight of each sample point according to the proportion of the ICG spectrum curve, set the light propagation depth to 2mm, and set the scattering coefficient of this layer to 10mm -1 , the absorption coefficient is 10cm -1 ; Use a camera to record the two-dimensional spatial distribution of fluorescence, and use a power meter to measure the power of fluorescence. As the initial state of the simulation, the fluorescence propagation of 5 sample points is simulated, and the fluorescence simulation results are superimposed according to the spectral weight to obtain the power change amplitude of the fluorescence after passing through a depth of 2mm.

[0068] The process of measuring fluorescence power using a power meter includes: placing the power meter at a distance of 100mm from the fluorescent sample, adding an 850nm narrowband filter in front of the power meter sensor, and reading the power meter value; in addition, using a spectrometer to measure and record the wavelength of the illumination light and the fluorescence emission wavelength.

[0069] According to the power variation amplitude of the excitation light and the power variation amplitude of the fluorescence, the power of the excitation light after passing through a depth of 2 mm is calculated, and then the power of the fluorescence after passing through a depth of 2 mm is calculated.

[0070] The set depth range was extended to 10 mm, the sampling step was set to 0.1 mm, and 100 depth sample points were obtained. Through fluorescence simulation, the fluorescence distribution of each depth layer was obtained, forming the depth-related data set of the final fluorescence emission power.

[0071] Expand the range of the scatter coefficient setting to 50mm -1 , the sampling step size is set to 1 / mm, and 50 scattering coefficient sample points are obtained. Through fluorescence simulation, the fluorescence distribution of each scattering coefficient is obtained, forming a scattering coefficient related data set of the final fluorescence emission power.

[0072] Expand the absorption coefficient range to 50cm -1 , the sampling step size is set to 1 / cm, and 50 absorption coefficient sample points are obtained. Through fluorescence simulation, the fluorescence distribution of each absorption coefficient is obtained, forming the absorption coefficient related data set of the final fluorescence emission power.

[0073] The final fluorescence emission power is affected by many factors, including depth, scattering, and absorption, as well as concentration, excitation intensity, and other factors. In addition, the fluorescence emission power under the combined influence of multiple factors is also different. By adjusting multiple parameters and undergoing computer simulation, a more comprehensive fluorescence emission data set can be obtained.

[0074] Example 2

[0075] The number of photons is associated with the initial fluorescence emission power of the simulation. First, the power per unit area of ​​the excitation light is detected and converted into the number of photons per unit area per unit time according to the wavelength of 785nm; the number of excitation photons is multiplied by the excitation efficiency to obtain the number of fluorescence emission photons per unit area per unit time; the number of fluorescence emission photons can be used as the initial state of the photon propagation calculation simulation to calculate the expanded fluorescence data set. The number of fluorescence emission photons is weighted according to the spectral curve and converted into fluorescence emission power, and finally detected using a power meter to achieve the association between fluorescence emission power and the number of fluorescence photons.

[0076] Specifically, the power meter was placed 2 mm above the sample plane, and the power reading at this time was recorded as 15 nW. The wavelength of the excitation light recorded by the spectrometer was 785 nm. The number of photons can be calculated from E = nhν to be 5.92 × 10 9 ; Assuming the fluorescence quantum yield of the fluorescent molecule is 0.01, the number of fluorescence emission photons is 5.92×10 7 , thus obtaining the initial states of excitation light and fluorescence, which can be used to simulate the extended fluorescence data set.

[0077] The set depth range was extended to 10 mm, the sampling step was set to 0.1 mm, and 100 depth sample points were obtained. Through fluorescence simulation, the fluorescence distribution of each depth layer was obtained, forming the depth-related data set of the final fluorescence emission power.

[0078] Expand the range of the scatter coefficient setting to 50mm -1 , the sampling step size is set to 1 / mm, and 50 scattering coefficient sample points are obtained. Through fluorescence simulation, the fluorescence distribution of each scattering coefficient is obtained, forming a scattering coefficient related data set of the final fluorescence emission power.

[0079] Expand the absorption coefficient range to 50cm -1 , the sampling step size is set to 1 / cm, and 50 absorption coefficient sample points are obtained. Through fluorescence simulation, the fluorescence distribution of each absorption coefficient is obtained, forming the absorption coefficient related data set of the final fluorescence emission power.

[0080] The final fluorescence emission power is affected by many factors, including depth, scattering, and absorption, as well as concentration, excitation intensity, and other factors. In addition, the fluorescence emission power under the combined influence of multiple factors is also different. By adjusting multiple parameters and undergoing computer simulation, a more comprehensive fluorescence emission data set can be obtained.

[0081] In summary, the present invention provides a method for acquiring fluorescence emission intensity based on an enhanced fluorescence data set. The present invention combines experimental acquisition and simulation calculation, not only providing accurate measurement of the baseline fluorescence emission intensity, but also obtaining illumination light changes and fluorescence changes by expanding the depth of fluorescent molecules, scattering coefficients and / or absorption coefficients, so as to enhance the fluorescence data set and expand the parameter range of the fluorescence data set. The present invention can act on the fluorescence data set according to the changes in fluorescence emission intensity and calculate the final fluorescence emission intensity. The present invention not only takes into account the luminescence characteristics of the fluorescent molecules themselves, but also takes into account the influence of biological tissues on the fluorescence signal, thereby realizing a comprehensive simulation of the fluorescence imaging process.

[0082] Accordingly, the present application also provides an electronic device, comprising: one or more processors; a memory for storing one or more programs; when the one or more programs are executed by the one or more processors, the one or more processors implement the above-mentioned method for acquiring fluorescence emission intensity based on enhanced fluorescence data set. Figure 4 As shown, it is a hardware structure diagram of any device with data processing capability in which the fluorescence emission intensity acquisition method based on enhanced fluorescence data set provided by an embodiment of the present invention is located, except Figure 4 In addition to the processor, memory and network interface shown, any device with data processing capability in which the apparatus in the embodiment is located may also include other hardware, generally based on the actual functions of the device with data processing capability, which will not be described in detail.

[0083] Accordingly, the present application also provides a computer-readable storage medium having computer instructions stored thereon, which, when executed by a processor, implement the above-mentioned method for acquiring fluorescence emission intensity based on enhanced fluorescence data sets. The computer-readable storage medium may be an internal storage unit of any device with data processing capabilities described in any of the foregoing embodiments, such as a hard disk or a memory. The computer-readable storage medium may also be an external storage device, such as a plug-in hard disk, a smart memory card (Smart Media Card, SMC), an SD card, a flash card (Flash Card), etc. equipped on the device. Furthermore, the computer-readable storage medium may also include both an internal storage unit of any device with data processing capabilities and an external storage device. The computer-readable storage medium is used to store the computer program and other programs and data required by any device with data processing capabilities, and may also be used to temporarily store data that has been output or is to be output.

[0084] Those skilled in the art will readily appreciate other embodiments of the present application after considering the description and practicing the contents disclosed herein. The present application is intended to cover any variations, uses or adaptations of the present application, which follow the general principles of the present application and include common knowledge or customary technical means in the art that are not disclosed in the present application. The description and examples are intended to be exemplary only.

[0085] It should be understood that the present application is not limited to the exact construction that has been described above and shown in the drawings, and that various modifications and changes may be made without departing from the scope thereof.

Claims

1. A method for acquiring fluorescence emission intensity based on enhanced fluorescence data set, characterized in that: The method comprises: Fluorescence detection of fluorescent molecules; under the illumination of fixed intensity excitation light, the fluorescence image of the fluorescent molecules is recorded by a fluorescence camera, the illumination light power is recorded by a power meter, the reference fluorescence emission power is obtained, and the illumination light wavelength and the fluorescence emission wavelength are recorded by a spectrometer, thereby obtaining a fluorescence data set as a fluorescence reference; Extending the depth of fluorescent molecules, scattering coefficients and / or absorption coefficients, illumination changes and fluorescence changes to enhance fluorescence data sets; The final fluorescence emission intensity is obtained by multiplying the baseline fluorescence emission intensity with the illumination light power change and the fluorescence power change.

2. The method for acquiring fluorescence emission intensity based on enhanced fluorescence data set according to claim 1, characterized in that: Also includes: Record the position of fluorescent molecules, the concentration of fluorescent molecules, the position of the fluorescence camera, the position of the power meter, and the position of the spectrometer.

3. The method for acquiring fluorescence emission intensity based on enhanced fluorescence data set according to claim 1, characterized in that: The process of obtaining the baseline fluorescence emission power includes: A simulated fluorescent light source is used, the light intensity of the simulated fluorescent light source is adjusted, and then a fluorescent camera and a power meter are used to detect and collect multiple sets of sample points of fluorescent emission power and fluorescent grayscale value, so as to obtain a mapping relationship between fluorescent emission power and fluorescent grayscale value; The grayscale value of the fluorescence image is read, and the reference fluorescence emission power is obtained according to the mapping relationship between the fluorescence emission power and the fluorescence grayscale value.

4. The method for acquiring fluorescence emission intensity based on enhanced fluorescence data set according to claim 1, characterized in that: Also includes: Obtain baseline fluorescence emission intensity, including: A filter that can eliminate excitation light is placed in front of the power meter, so that the detection result of the power meter is not affected by the excitation light; The fluorescence emission power is collected by a power meter, and the reference fluorescence emission intensity is calculated based on the fluorescence spectrum integral relationship.

5. The method for acquiring fluorescence emission intensity based on enhanced fluorescence data set according to claim 1, characterized in that: Expanding the depth of fluorescent molecules, scattering coefficients and / or absorption coefficients, obtaining illumination light changes and fluorescence changes including: Set the fluorescence molecule depth z and scattering coefficient μ s and / or absorption coefficient μ a range, and the illumination light wavelength λ i , calculate the change of the illumination light after propagating through the depth, recorded as the illumination light power change δI; Set the fluorescence molecule depth z and scattering coefficient μ s and / or absorption coefficient μ a The range of fluorescence emission wavelength λ f , calculate the change of fluorescence after propagation through this depth, and record it as the fluorescence power change δF.

6. The method for acquiring fluorescence emission intensity based on enhanced fluorescence data set according to claim 1, characterized in that: The depth range is expanded to δz, the depth sampling rate is set to σz, a series of depth values ​​in the range of 0 to δz with a resolution of σz are obtained, and the illumination light power change δI and the fluorescence power change δF corresponding to each depth value are calculated; Expand the scattering range to δμ s , set the scattering sampling rate to σμ s , we get 0~δμ s interval and resolution is σμ s A series of scattering values ​​are obtained, and the illumination light power change δI and fluorescence power change δF corresponding to each scattering value are calculated; or, Extending the absorption range to δμ a , set the absorption sampling rate to σμ a , we get 0~δμ a interval and resolution is σμ a A series of absorption values ​​are obtained, and the illumination light power change δI and fluorescence power change δF corresponding to each absorption value are calculated.

7. The method for acquiring fluorescence emission intensity based on enhanced fluorescence data set according to claim 1, characterized in that: The expression for the final fluorescence emission intensity obtained by multiplying the reference fluorescence emission intensity by the illumination light power change and the fluorescence power change is as follows: P2=P1×δI×δF Where P2 represents the final fluorescence emission intensity, P1 represents the reference fluorescence emission intensity, δI represents the illumination light power change, and δF represents the fluorescence power change.

8. An electronic device, comprising a memory and a processor, characterized in that: The memory is coupled to the processor; wherein the memory is used to store program data, and the processor is used to execute the program data to implement the method for acquiring fluorescence emission intensity based on enhanced fluorescence data set as described in any one of claims 1 to 7.

9. A computer-readable storage medium having a computer program stored thereon, characterized in that: When the program is executed by a processor, the method for acquiring fluorescence emission intensity based on an enhanced fluorescence data set as described in any one of claims 1 to 7 is implemented.

10. A computer program product comprising a computer program / instructions, characterized in that When the computer program / instruction is executed by a processor, the method for acquiring fluorescence emission intensity based on an enhanced fluorescence data set described in any one of claims 1 to 7 is implemented.

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