Cosmetic containing collagen and application of reverse micelle carrier in preparation of cosmetic for improving collagen intradermal retention volume

Abstract

The invention relates to the field of cosmetics, and relates to a collagen-containing cosmetic, in particular to application of a reverse micelle carrier in preparation of a cosmetic for improving collagen intradermal retention volume. The cosmetic comprises a collagen composite raw material, the collagen composite raw material comprises the following components in percentage by mass: 53.55-80% of grease; 15 to 60 percent of phospholipid; 0.5 to 1.5 percent of an antioxidant; 0.1 to 2 percent of water; and 0.01% to 0.5% of collagen; the grease is prepared from any one of isopropyl myristate, isopropyl laurate or isopropyl acetate; the phospholipid comprises lecithin, and the purity of the lecithin is 90% or above. The retention amount of the collagen in the cosmetic containing the collagen in the skin can be increased, so that the collagen permeates into the skin epidermis or dermis and accumulates in the skin layer; the real collagen supplementing effect of cosmetics is greatly improved, and the skin young state is facilitated.

Description

Technical Field

[0001] The present application relates to the field of cosmetics, and in particular, to a cosmetic containing collagen, and an application of a reverse micelle carrier in the preparation of a cosmetic for increasing the intradermal retention of collagen. Background Art

[0002] Studies have shown that collagen synthesis slows down significantly at the age of 25, and the collagen content reaches a peak at the age of 30 and then declines. At the same time, modern unhealthy lifestyles and environmental damage further cause the loss of collagen to be pushed forward to between the ages of 22 and 25. Therefore, supplementing collagen is the key to youthful skin.

[0003] However, due to its own structure, collagen is highly water-soluble and poorly oil-soluble, making it difficult to penetrate the natural lipid barrier formed by the stratum corneum. Collagen can only truly exert its efficacy when it penetrates into the epidermis or dermis and accumulates in this skin layer.

[0004] Known prior art, such as Chinese patent CN117417435A, currently generally adopts a method of modifying collagen with a hydrophobic modifier to enhance its oil solubility, but the introduced organic reagent is easy to remain and cause skin problems. Summary of the invention

[0005] The purpose of the embodiments of the present application is to provide a cosmetic containing collagen and the use of a reverse micelle carrier in the preparation of a cosmetic for increasing the intradermal retention of collagen.

[0006] In a first aspect, the present application provides a cosmetic containing collagen, wherein the cosmetic containing collagen includes a collagen composite raw material; the collagen composite raw material forms a reverse micelle carrier structure;

[0007] According to the percentage by mass, the collagen composite raw material includes: 53.55% to 80% oil; 15% to 60% phospholipids; 0.5% to 1.5% antioxidant; 0.1% to 2% water; and 0.01% to 0.5% collagen;

[0008] The oil includes any one of isopropyl myristate, isopropyl laurate or isopropyl acetate;

[0009] Antioxidants include: butylated hydroxyanisole;

[0010] The phospholipids include lecithin, and the purity of lecithin is more than 90%.

[0011] In the above technical scheme, by adopting: 53.55% to 80% oil; 15% to 60% phospholipids; 0.5% to 1.5% antioxidants; 0.1% to 2% water; and 0.01% to 0.5% collagen; a reverse micelle carrier structure containing collagen can be formed; this structure can simultaneously realize the transport of collagen to penetrate the natural lipid barrier formed by the stratum corneum, and can increase the retention of collagen in the skin. Applying this collagen raw material to cosmetics containing collagen can increase the retention of collagen in the skin, so that the collagen can penetrate into the epidermis or dermis of the skin and accumulate in the skin layer to better exert its efficacy; thereby, the real effect of cosmetics in supplementing collagen can be greatly improved, which is beneficial to the youthful state of the skin.

[0012] In other embodiments of the present application, the viscosity of the collagen composite material is 30,000 Pa·s to 60,000 Pa·s.

[0013] In other embodiments of the present application, the pore size of the collagen composite material is about 300 nm to 500 nm.

[0014] In a second aspect, the present application provides a reverse micelle carrier for use in preparing cosmetics for increasing the intradermal retention of collagen.

[0015] The reverse micelle carrier comprises, by weight percentage, 53.55% to 80% oil; 15% to 60% phospholipid; 0.5% to 1.5% antioxidant; 0.1% to 2% water; and 0.01% to 0.5% collagen;

[0016] The oil includes any one of isopropyl palmitate, isopropyl myristate, isopropyl laurate or isopropyl acetate;

[0017] The antioxidant includes any one of tert-butylhydroquinone, butylated hydroxyanisole, tocopheryl acetate, propyl gallate or butylated hydroxytoluene;

[0018] The phospholipids include lecithin, and the purity of lecithin is more than 90%.

[0019] In other embodiments of the present application, the viscosity of the reverse micelle carrier is 30,000 Pa·s to 60,000 Pa·s.

[0020] In other embodiments of the present application, the pore size of the reverse micelle carrier is about 300 nm to 500 nm.

[0021] In other embodiments of the present application, the preparation of the reverse micelle carrier includes:

[0022] The phospholipids, oils and antioxidants are mixed to prepare an oil phase mixture;

[0023] Mixing collagen with water to prepare an aqueous phase mixture;

[0024] The aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure.

[0025] In the above technical solution, the aqueous phase mixture obtained by mixing collagen with water is added dropwise to the oil phase mixture obtained by mixing phospholipids with oils to form a viscous reverse micelle structure. This viscous reverse micelle structure can be used as a carrier of collagen, and can carry collagen below the natural lipid barrier formed by the stratum corneum; at the same time, this viscous reverse micelle structure can increase the retention of collagen in the skin; compounding this collagen composite raw material into cosmetics can effectively improve the effect of cosmetics in supplementing collagen.

[0026] In other embodiments of the present application, the aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure, comprising:

[0027] The aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure, and the viscosity is controlled to be 30000 Pa·s to 60000 Pa·s.

[0028] In other embodiments of the present application, the collagen is mixed with water to prepare an aqueous phase mixture, comprising:

[0029] In terms of mass percentage, collagen and water are mixed in the ratio of (0.1% to 25%): (75% to 99.9%);

[0030] Optionally, the phospholipids, oils and antioxidants are mixed to prepare an oil phase mixture, comprising:

[0031] Calculated by mass percentage, the phospholipids, oils and antioxidants are mixed in the ratio of (15% to 60%): (53.55% to 80%): (0.5% to 1.5%).

[0032] In other embodiments of the present application, the aqueous phase mixture is added dropwise to the oil phase mixture, comprising:

[0033] In terms of mass percentage, 0.1% to 2% of the water phase mixture is added dropwise to 98% to 99.9% of the oil phase mixture and stirred. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0035] Figure 1 This is a transmission electron micrograph of the collagen composite material prepared in Example 1 of the present application;

[0036] Figure 2 This is a transmission electron microscopy image of the collagen composite material prepared in Example 2 of the present application;

[0037] Figure 3 This is a transmission electron micrograph of the collagen composite material prepared in Example 3 of the present application. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments.

[0039] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

[0040] Through research, it was found that by wrapping collagen with phospholipids, a reverse micelle structure can be formed. The reverse micelle structure can simultaneously carry collagen and increase the retention of collagen in the skin. Applying this collagen raw material to cosmetics containing collagen can increase the retention of collagen in the skin. Collagen can only play a better role if it penetrates into the epidermis or dermis of the skin and accumulates in this skin layer. This greatly enhances the real effect of collagen supplementation and is beneficial to youthful skin.

[0041] Based on this, the embodiment of the present application provides a cosmetic containing collagen, wherein the cosmetic containing collagen includes a collagen composite raw material; the collagen composite raw material forms a reverse micelle carrier structure;

[0042] According to the percentage by mass, the collagen composite raw material includes: 53.55% to 80% oil; 15% to 60% phospholipids; 0.5% to 1.5% antioxidant; 0.1% to 2% water; and 0.01% to 0.5% collagen;

[0043] The oil includes any one of isopropyl myristate, isopropyl laurate or isopropyl acetate;

[0044] Antioxidants include: butylated hydroxyanisole;

[0045] The phospholipids include lecithin, and the purity of lecithin is more than 90%.

[0046] In the above technical scheme, by adopting: 53.55% to 80% oil; 15% to 60% phospholipids; 0.5% to 1.5% antioxidants; 0.1% to 2% water; and 0.01% to 0.5% collagen; a reverse micelle carrier structure containing collagen can be formed; this structure can simultaneously realize the transport of collagen to penetrate the natural lipid barrier formed by the stratum corneum, and can increase the retention of collagen in the skin. Applying this collagen raw material to cosmetics containing collagen can increase the retention of collagen in the skin, so that the collagen can penetrate into the epidermis or dermis of the skin and accumulate in the skin layer to better exert its efficacy; thereby, the real effect of cosmetics in supplementing collagen can be greatly improved, which is beneficial to the youthful state of the skin.

[0047] For example, in some embodiments of the present application, the collagen composite raw material includes, by weight percentage:

[0048] 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 70%, 71%, 72%, 75%, 78%, 80% or a range between any two of the foregoing values;

[0049] 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5% or the range between any two of the foregoing values ​​of antioxidants;

[0050] 16%, 18%, 20%, 22%, 25%, 30%, 35%, 40%, 45%, 50%, 52%, 55%, 58%, 60% or a range between any two of the foregoing phospholipids;

[0051] 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.2%, 1.5%, 1.8%, 2% or a range between any two of the foregoing water; and

[0052] 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5% or a range between any two of the foregoing values ​​of collagen.

[0053] Optionally, in some embodiments of the present application, the above-mentioned oil includes any one of isopropyl palmitate, isopropyl myristate, isopropyl laurate or isopropyl acetate.

[0054] Optionally, in some embodiments of the present application, the above-mentioned antioxidant includes: any one of tert-butylhydroquinone, butylated hydroxyanisole, tocopherol acetate, propyl gallate or butylated hydroxytoluene.

[0055] Illustratively, in some embodiments of the present application, the above-mentioned oil is isopropyl palmitate; or in some embodiments of the present application, the above-mentioned oil is isopropyl myristate; or in some embodiments of the present application, the above-mentioned oil is isopropyl laurate; or in some embodiments of the present application, the above-mentioned oil is isopropyl acetate.

[0056] Illustratively, in some embodiments of the present application, the above-mentioned antioxidant is tert-butylhydroquinone; or in some embodiments of the present application, the above-mentioned antioxidant is butylated hydroxyanisole; or in some embodiments of the present application, the above-mentioned antioxidant is tocopherol acetate; or in some embodiments of the present application, the above-mentioned antioxidant is propyl gallate; or in some embodiments of the present application, the above-mentioned antioxidant is butylated hydroxytoluene.

[0057] In some embodiments of the present application, the above-mentioned phospholipids include lecithin, and the purity of the lecithin is above 90%.

[0058] Further optionally, illustratively, in some embodiments of the present application, the purity of the lecithin is 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% or a range between any two of the foregoing values.

[0059] Furthermore, in some embodiments of the present application, the viscosity of the collagen composite material is 30,000 Pa·s to 60,000 Pa·s.

[0060] When the viscosity is between 30,000 Pa·s and 60,000 Pa·s, the structure of the reverse micelles is moderately tight. This viscosity indicates that the interaction between the reverse micelles has reached a state of equilibrium, neither being too loose to cause premature release of collagen, nor being too tight to hinder the contact between collagen and the skin; the viscosity helps control the release rate of collagen so that it can penetrate into the skin slowly and continuously. If the viscosity is too low, collagen may be released all at once and cannot penetrate effectively; if the viscosity is too high, the release of collagen will be severely hindered and not conducive to penetration; collagen can better adapt to the intradermal environment, allowing collagen to stay in the skin for a longer time, thereby increasing the intradermal retention.

[0061] Viscosity is one of the important physical properties of fluids. The flow patterns of fluids can be divided into Newtonian fluids, where the shear stress required for flow does not change with the flow velocity, and non-Newtonian fluids, where the shear stress required for flow changes with the flow velocity. Pseudoplastic fluids among non-Newtonian fluids have the characteristic of shear thinning.

[0062] In some embodiments of the present application, assuming that the collagen composite material is a non-Newtonian fluid, the viscosity thereof is tested as follows:

[0063] Before measuring the sample viscosity, it is necessary to carefully select the rotor specifications and models that match the sample characteristics and the estimated viscosity range based on the preliminary evaluation results of the sample viscosity, and then accurately install the selected rotor to the rotating shaft of the viscometer. Place the container containing the sample to be tested in a temperature control device, set and maintain a constant temperature environment, and after the sample temperature reaches a thermal equilibrium with the preset measurement temperature and remains stable for a period of time, use professional pipetting equipment to carefully transfer the sample to the measuring cylinder of the viscometer at a slow, steady and controllable rate, and continue filling until the sample liquid level reaches and submerges the specified scale line position of the rotor. If bubbles are accidentally mixed in, stop the filling operation immediately and place the sample on a stable table. The length of time for standing is reasonably determined based on the number and size of the bubbles, usually within the range of 3-5 minutes, to wait for the bubbles to float up and escape naturally. After completing the sample filling and bubble troubleshooting, turn on the viscometer power supply, and according to the established measurement scheme and sample characteristics, accurately set the measurement speed on the viscometer operation panel, wait for the data presented on the viscometer reading display to stabilize, accurately record the viscosity value shown on the display at this moment, and strictly follow this process specification, and repeat the measurement operation three times. Exemplarily, in some embodiments of the present application, the viscosity of the collagen composite material is 30000Pa·s, 35000Pa·s, 40000Pa·s, 42000Pa·s, 45000Pa·s, 48000Pa·s, 50000Pa·s, 52000Pa·s, 55000Pa·s, 58000Pa·s, 60000Pa·s or the range between any two of the foregoing values.

[0064] In a second aspect, some embodiments of the present application provide a use of a reverse micelle carrier in the preparation of a cosmetic for increasing the intradermal retention of collagen.

[0065] Furthermore, in some embodiments of the present application, the reverse micelle carrier includes, by mass percentage: 53.55% to 80% oil; 15% to 60% phospholipids; 0.5% to 1.5% antioxidant; 0.1% to 2% water; and 0.01% to 0.5% collagen.

[0066] Further, in some embodiments of the present application, the oil includes: any one of isopropyl palmitate, isopropyl myristate, isopropyl laurate or isopropyl acetate;

[0067] Further, in some embodiments of the present application, the antioxidant includes: any one of tert-butylhydroquinone, butylated hydroxyanisole, tocopherol acetate, propyl gallate or butylated hydroxytoluene;

[0068] Furthermore, in some embodiments of the present application, the phospholipid includes lecithin, and the purity of the lecithin is above 90%.

[0069] Furthermore, in some embodiments of the present application, the viscosity of the reverse micelle carrier is 30,000 Pa·s to 60,000 Pa·s.

[0070] Furthermore, in some embodiments of the present application, the pore size of the reverse micelle carrier is about 300 nm to 500 nm.

[0071] Further optionally, in some embodiments of the present application, the above-mentioned collagen can be selected from recombinant collagen; illustratively, it includes any one of type I recombinant collagen, type II recombinant collagen or type III recombinant collagen.

[0072] Further optionally, in some embodiments of the present application, the amino acid sequence of the above-mentioned recombinant collagen includes amino acids 950-1075 of type I collagen, and amino acids 594-729 and 1193-1199 of type III collagen.

[0073] Furthermore, the above-mentioned collagen can be purchased commercially.

[0074] Illustratively, in some embodiments of the present application, the above-mentioned collagen can be selected from HydroCol-y recombinant collagen produced by Best Pharmaceuticals (Guangzhou) Co., Ltd.

[0075] Further, in some embodiments of the present application, the preparation of the reverse micelle carrier includes:

[0076] The phospholipids and the oil antioxidant are mixed to prepare an oil phase mixture;

[0077] Mixing collagen with water to prepare an aqueous phase mixture;

[0078] The aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure.

[0079] In the above technical scheme, by adding the above-mentioned aqueous phase mixture to the oil phase mixture to form a viscous reverse micelle structure, collagen can be transported and the retention amount of collagen in the skin can be increased at the same time. This collagen raw material is applied to cosmetics containing collagen, which can increase the retention amount of collagen in the skin of cosmetics containing collagen. Collagen can better exert its efficacy only when it penetrates into the epidermis or dermis of the skin and accumulates in the skin layer; thereby, the real effect of collagen supplementation is greatly improved, which is beneficial to the youthful state of the skin.

[0080] Further, in some embodiments of the present application, in other embodiments of the present application, the aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure, comprising:

[0081] The aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure, and the viscosity is controlled to

[0082] Further, in some embodiments of the present application, the collagen is mixed with water to prepare an aqueous phase mixture, comprising:

[0083] In terms of mass percentage, collagen and water are mixed in the ratio of (0.1% to 25%): (75% to 99.9%).

[0084] For example, in some embodiments of the present application, collagen is mixed with water to prepare an aqueous phase mixture, comprising:

[0085] In terms of mass percentage, collagen is mixed with water according to (0.1%, 0.2%, 0.3%, 0.5%, 0.6%, 0.7%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 20%, 25% or the range between any two of the foregoing values): (75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.9% or the range between any two of the foregoing values).

[0086] Further, in some embodiments of the present application, phospholipids, oils and antioxidants are mixed to prepare an oil phase mixture, comprising:

[0087] Calculated by mass percentage, the phospholipids, oils and antioxidants are mixed in the ratio of (15% to 60%): (53.55% to 80%): (0.5% to 1.5%).

[0088] For example, in some embodiments of the present application, phospholipids, oils and antioxidants are mixed to prepare an oil phase mixture, including:

[0089] In terms of mass percentage, the phospholipids, oils and antioxidants are mixed according to (15%, 18%, 20%, 30%, 35%, 40%, 45%, 46%, 47%, 48%, 49%, 52%, 55%, 60% or the range between any two of the aforementioned values): (55%, 58%, 60%, 62%, 65%, 70%, 75%, 78%, 80% or the range between any two of the aforementioned values): (0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5% or the range between any two of the aforementioned values).

[0090] Further, in some embodiments of the present application, the aqueous phase mixture is added dropwise to the oil phase mixture, comprising:

[0091] In terms of mass percentage, 0.1%-2.0% of the water phase mixture is added dropwise into 98%-99.9% of the oil phase mixture and stirred.

[0092] For example, in some embodiments of the present application, the aqueous phase mixture is added dropwise to the oil phase mixture, comprising:

[0093] In terms of mass percentage, 0.1%, 0.2%, 0.5%, 1%, 1.1%, 1.2%, 1.3%, 1.5%, 1.6%, 1.7%, 1.8%, and 2.0% of the aqueous phase mixture were added dropwise to the 98% to 99.9% oil phase mixture and stirred.

[0094] In other optional embodiments of the present application, the above-mentioned essential oil cosmetics containing collagen may also include other applicable cosmetic bases commonly used in the art (at least one of essence water, essence lotion, and facial cream), and the above-mentioned collagen composite raw materials are compounded with the cosmetic base to form cosmetics.

[0095] Further optionally, in some embodiments of the present application, in the above-mentioned cosmetics, the addition amount of the aforementioned collagen composite raw material is 0.01% to 30% by mass; illustratively, in some embodiments of the present application, in the above-mentioned cosmetics, the addition amount of the aforementioned collagen composite raw material is 0.01%, 0.02%, 0.05%, 0.08%, 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4%, 5%, 8%, 10%, 12%, 15%, 18%, 20%, 22%, 25%, 28%, 30% or the range between any two of the aforementioned values.

[0096] For example, a formula of an essential oil cosmetic containing the aforementioned collagen composite raw material is shown in Table 1 below:

[0097] Table 1

[0098] Raw material name Mass percentage (%) Lignum Meadowfoam Seed Oil 30 Isononyl Isononanoate 20 Squalane 20 Caprylic / capric triglyceride 10 Jojoba Seed Oil 10 Camellia Seed Oil 7 Ascorbyl Tetraisopalmitate 2 Collagen compound raw material 1

[0099] Further optionally, in some embodiments of the present application, the above-mentioned cosmetic base may include moisturizers, emulsifiers, thickeners, etc. commonly used in the art.

[0100] The features and performance of the present application are further described in detail below in conjunction with the embodiments:

[0101] Examples and Comparative Examples

[0102] A collagen composite raw material is provided, and the proportions and compositions of various embodiments and comparative examples are shown in Table 2;

[0103] Table 2

[0104]

[0105]

[0106] Preparation process:

[0107] Weigh each raw material according to the composition ratio of each embodiment or comparative example in Table 2 above:

[0108] Each example was prepared according to the following preparation method:

[0109] S1, mixing phospholipids, oils and antioxidants, heating and stirring to form a uniform oil phase mixture;

[0110] S2, mixing and stirring the various raw materials of the aqueous phase to form a uniform aqueous phase mixture;

[0111] S3. Add the uniform aqueous phase mixture obtained in step S2 dropwise into the uniform oil phase mixture obtained in step S2, and stir at room temperature until viscous reverse micelles are formed.

[0112] The comparative example was prepared according to the following preparation method:

[0113] S1. Mix and stir the various raw materials of the aqueous phase to form a uniform aqueous phase mixture.

[0114] Performance Testing

[0115] 1. In vitro transdermal test:

[0116] The samples prepared in the above-mentioned embodiments and comparative examples were respectively subjected to in vitro pig skin permeation experiments using a vertical Franz diffusion cell method:

[0117] The suckling pig skin was fixed in the receiving chamber and the supply chamber, and 0.5 g of the collagen composite raw material of each embodiment and the collagen aqueous solution sample of Comparative Example 1 were taken into the supply chamber, and a PBS buffer solution with a pH of 7.4 was used as the receiving solution, and stirred and diffused at 32°C. After 24 hours, the skin was removed, washed and cut into pieces, and an appropriate amount of PBS buffer solution was added. After shaking for half an hour, the supernatant was taken for HPLC detection and analysis, and the intradermal retention per unit area of ​​collagen was calculated. The experimental results are shown in Table 3.

[0118] Table 3

[0119]

[0120] From the test results in Table 3 above, it can be seen that:

[0121] The results of intradermal retention showed:

[0122] Compared with the collagen aqueous solution provided in Comparative Example 1, the collagen composite raw materials provided in each embodiment have a greater intradermal retention of collagen than Comparative Example 1; thus, it is shown that the collagen composite raw materials provided in each embodiment can effectively increase the intradermal retention of collagen. The increase in the intradermal retention of collagen in each embodiment relative to Comparative Example 1 is 2.99 to 4 times.

[0123] Furthermore, by comparing the various embodiments, it can be seen that the collagen composite material provided in Example 1 has the largest intradermal retention of collagen, which can be explained that the collagen composite material provided in Example 1 has the best improvement in the intradermal retention of collagen. It can be explained that when oil, phospholipids, antioxidants, water and collagen are mixed in a mass percentage of 69.3%: 28.5%: 1.5%: 1.35%: 0.15%, the intradermal retention of collagen is the best. Compared with Comparative Example 1, Example 1 can increase the intradermal retention of collagen by more than 4 times.

[0124] The viscosity in Table 3 is measured by the viscosity test method described above. As can be seen from Table 3, the viscosity of the collagen composite material increases with the increase of the phospholipid content, while the viscosity of the collagen composite material is not positively correlated with the intradermal retention amount. The present application scheme selects a relatively moderate viscosity range, which will be more conducive to promoting collagen penetration and increasing its retention amount in the skin.

[0125] Adding the above-mentioned collagen composite raw materials to cosmetics containing collagen can effectively increase the intradermal retention of collagen in the cosmetics, thereby helping to enhance the effect of the cosmetics in supplementing collagen, allowing it to truly exert its effect, thereby maximizing its efficacy and thus improving the efficacy of the cosmetics.

[0126] 2. Microstructure characterization

[0127] The microstructures of the collagen composite materials provided in Example 1, Example 2, and Example 3 were observed using a transmission electron microscope:

[0128] 10 μl of each sample was respectively added to the copper grid for precipitation for 1 min, and the floating liquid was removed by filter paper; 10 μl of uranyl acetate staining solution was added to the copper grid for precipitation for 1 min, and the floating liquid was removed by filter paper; the mixture was dried at room temperature for several minutes; the electron microscope was used for imaging at 80 kV-120 kV, and the images were collected for analysis.

[0129] Transmission electron microscopy results are shown in the instruction manual. Figure 1 -Attached Figure 3 .

[0130] From the instruction manual Figure 1 -Attached Figure 3 From the transmission electron microscopy images we can see that:

[0131] The collagen composite raw material provided in Examples 1-3 presents a reverse micelle structure; the pore size of the reverse micelle structure loaded with collagen is about 300nm to 500nm. This structure with a nanoscale pore size is more conducive to carrying collagen into the skin and increasing the retention of collagen in the skin. This is because the skin has appendage channels such as hair follicles and sweat glands. The diameter of the hair follicles is about 50 to 100 μm, and the diameter of the sweat gland duct is about 10 to 20 μm. The reverse micelle particle size of 300nm to 500nm is relatively small, and can utilize the gaps around these appendage channels or aggregate in the channels; the size of the endocytic vesicles of skin cells is usually around 100 to 300nm. Although the reverse micelle particle size of 300nm to 500nm is slightly larger than the general endocytic vesicle, it is still within the effective range that cells can recognize and ingest. When the reverse micelle particle size is in this range, skin cells can take up the reverse micelles through some special endocytosis methods; once collagen enters the skin, the reverse micelles with a particle size of 300nm to 500nm can, to a certain extent, prevent the collagen from being cleared too quickly by the metabolic process inside the skin.

[0132] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A cosmetic containing collagen, characterized in that: The cosmetics containing collagen include a collagen composite material; the collagen composite material forms a reverse micelle carrier structure; According to the percentage by mass, the collagen composite raw material comprises: 53.55% to 80% oil; 15% to 60% phospholipids; 0.5% to 1.5% antioxidant; 0.1% to 2% water; and 0.01% to 0.5% collagen; The oil includes: any one of isopropyl myristate, isopropyl laurate or isopropyl acetate; The antioxidants include: butylated hydroxyanisole; The phospholipids include lecithin, and the purity of the lecithin is above 90%.

2. The collagen-containing cosmetic according to claim 1, characterized in that: The viscosity of the collagen composite raw material is 30000 Pa·s to 60000 Pa·s.

3. The collagen-containing cosmetic according to claim 1, characterized in that: The pore size of the collagen composite raw material is 300nm-500nm.

4. Use of a reverse micelle carrier in the preparation of cosmetics for increasing the intradermal retention of collagen, characterized in that: The reverse micelle carrier comprises, by weight percentage: 53.55% to 80% oil; 15% to 60% phospholipids; 0.5% to 1.5% antioxidant; 0.1% to 2% water; and 0.01% to 0.5% collagen; The oil includes: any one of isopropyl myristate, isopropyl laurate or isopropyl acetate; The antioxidants include: butylated hydroxyanisole; The phospholipids include lecithin, and the purity of the lecithin is above 90%.

5. Use of the reverse micelle carrier according to claim 4 in the preparation of cosmetics for improving the intradermal retention of collagen, characterized in that: The viscosity of the reverse micelle carrier is 30000 Pa·s to 60000 Pa·s.

6. Use of the reverse micelle carrier according to claim 4 in the preparation of cosmetics for improving the intradermal retention of collagen, characterized in that: The pore size of the reverse micelle carrier is about 300nm to 500nm.

7. Use of the reverse micelle carrier according to claim 4 in the preparation of cosmetics for improving the intradermal retention of collagen, characterized in that: The preparation of the reverse micelle carrier comprises: mixing phospholipids, oils and antioxidants to prepare an oil phase mixture; Mixing collagen with water to prepare an aqueous phase mixture; The aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure.

8. Use of the reverse micelle carrier according to claim 7 in preparing cosmetics for improving the intradermal retention of collagen, characterized in that: The step of adding the aqueous phase mixture dropwise to the oil phase mixture to form a viscous reverse micelle structure comprises: The aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure, and the viscosity is controlled to be 30000 Pa·s to 60000 Pa·s.

9. Use of the reverse micelle carrier according to claim 7 in preparing cosmetics for improving the intradermal retention of collagen, characterized in that: The step of mixing collagen with water to obtain an aqueous phase mixture comprises: In the aqueous phase mixture, the collagen and the water are mixed in the ratio of (0.1% to 25%): (75% to 99.9%) by mass percentage; Optionally, the mixing of phospholipids, oils and antioxidants to obtain an oil phase mixture comprises: In terms of mass percentage, the phospholipid, the oil and the antioxidant are mixed in the ratio of (15% to 60%): (53.55% to 80%): (0.5% to 1.5%).

10. Use of the reverse micelle carrier according to claim 7 in preparing cosmetics for improving the intradermal retention of collagen, characterized in that: The step of adding the aqueous phase mixture dropwise to the oil phase mixture comprises: In terms of mass percentage, 0.1% to 2.0% of the aqueous phase mixture is added dropwise to 98% to 99.9% of the oil phase mixture, and stirred.

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