The invention discloses dog food capable of improving antioxidant ability and intestinal health conditions of dogs and a preparation method thereof. The dog food is manufactured by adding tea polyphenol with additive amount of reaching 0.1-1 part, by weight, into 100 parts, by weight, of dog food, and going through procedure of mixing, stirring, extruding, puffing and the like. The remaining components of the dog food comprise, by weight, 15-25 parts of refined rice, 15-25 parts of bean pulp, 15-35 parts of corn, 5-15 parts of wheatmeal, 10-25 parts of fish meal, 5-10 parts of yeast powder, 1-3 parts of calcium hydrophosphate, 0.1-0.5 part of edible salt, 0.1-0.5 part of vitamin premix, 0.1-0.5 part of mineral premix, and 0.05-0.15 part of potassium sorbate. According to the dog food, tea polyphenol is used as functional feed supplements in the production of commodity dog food. Consequently, the dog food can effectively improve the antioxidant ability and the intestinal health conditions of dogs, and achieves double effects of guaranteeing the quality of commodities and caring health in a functional mode.