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Protein-EGCG composite nanoparticle and antioxidant Pickering high internal phase emulsion

A technology of composite nanoparticle and protein solution, which is applied in the direction of emulsion delivery, anti-inflammatory agent, anti-toxic agent, etc. It can solve the problems of difficult slow-release targeting effect, high oil volume, difficult absorption, etc., and achieve superior antioxidant properties , Nutrient-rich, anti-oxidation effect

Active Publication Date: 2020-07-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high internal phase emulsion has a high oil volume and the oil phase is easy to oxidize and deteriorate, which limits its application in food, and many nutrients are fat-soluble active substances, which are not easily absorbed by the human body or fail prematurely in the human body. Slow release plays a targeted role

Method used

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  • Protein-EGCG composite nanoparticle and antioxidant Pickering high internal phase emulsion
  • Protein-EGCG composite nanoparticle and antioxidant Pickering high internal phase emulsion
  • Protein-EGCG composite nanoparticle and antioxidant Pickering high internal phase emulsion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A kind of nano particle, its preparation method comprises the following steps:

[0044] 1) Disperse 5 g of soybean β-conglycinin powder in 100 g of distilled water, stir at room temperature for 2 h, then add 1 drop of 0.08% sodium azide solution (to prevent the growth of microorganisms) with a concentration of 0.2 mol / L HCl solution and NaOH solution with a concentration of 0.2mol / L to adjust the pH of the solution to 7, stir for 2 hours, place in a refrigerator at 4°C overnight, then take it out from the refrigerator and return to room temperature, and centrifuge (8000rpm, 15min) to remove insoluble impurities to obtain A protein solution with a concentration of 5wt%;

[0045] 2) Disperse 0.4 g of EGCG powder in 20 g of distilled water, stir at room temperature for 2 h, and then place it in a refrigerator at 4° C. overnight to obtain an EGCG solution with a concentration of 2 wt %;

[0046]3) Take out 5 high-foot beakers with a capacity of 50mL and number them No. 1, ...

Embodiment 2

[0057] A kind of Pickering high internal phase emulsion, its preparation method comprises the following steps:

[0058] The protein particle solution of sample No. 1 in Example 1 and the protein-EGCG composite nanoparticle solution of samples No. 2 to No. 5 were mixed with linseed oil at a volume ratio of 1:4 and added to a homogenizer, and homogenized at 5000 rpm for 1 min to obtain Pickering high internal phase emulsion (the Pickering high internal phase emulsion corresponding to sample No. 1 does not have anti-oxidation function, and the Pickering high internal phase emulsion corresponding to samples No. 2 to 5 has anti-oxidation function).

[0059] Performance Testing:

[0060] The appearance picture and optical microscope picture of Pickering high internal phase emulsion Figure 5 shown.

[0061] The change of elastic modulus (G′, solid) and viscous modulus (G″, hollow) of Pickering high internal phase emulsion with frequency is shown in the figure Image 6 shown.

[...

Embodiment 3

[0070] Referring to the method of Example 2, the dispersed phase linseed oil was replaced by n-dodecane dissolved with 0.003 wt% β-carotene, and then the Pickering high internal phase emulsion was prepared.

[0071] Performance Testing:

[0072] The appearance of Pickering high internal phase emulsion after heat treatment (stored in 70°C electric thermostat for 18h) is as follows Figure 10 As shown, the retention rate of β-carotene is shown in Figure 11 Shown (a to f in the figure are marked for significant differences).

[0073] Depend on Figure 10 and Figure 11 It can be seen that after storage at 70°C for 18 hours, the stable high internal phase emulsion of No. 1 (without EGCG) particles, the stable high internal phase emulsion of No. 2, No. 3, No. 4, and No. 5 particles (protein-EGCG composite nanoparticles) , Tween 20 (2.0wt%) stable high internal phase emulsion, the beta-carotene retention rate in equal volume dispersion liquid n-dodecane is respectively 88.8%, 9...

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Abstract

The invention discloses a protein-EGCG composite nanoparticle and an antioxidant Pickering high internal phase emulsion. The preparation method of the protein-EGCG composite nanoparticle comprises thefollowing steps: 1) adding water into soybean beta-conglycinin for dispersion, and performing hydration to obtain a protein solution; 2) adding water into EGCG for dispersion to obtain an EGCG solution; 3) adding the protein solution into a polar organic solvent to denature the protein, and adding the EGCG solution to obtain a mixed solution; and 4) dialyzing the mixed solution to remove the polar organic solvent and free EGCG to obtain the protein-EGCG composite nanoparticle. The stabilizing agent in the antioxidant Pickering high internal phase emulsion disclosed by the invention is the protein-EGCG composite nanoparticle. The protein-EGCG composite nanoparticle disclosed by the invention has good biocompatibility, and a Pickering high internal phase emulsion prepared by using the protein-EGCG composite nanoparticle as a stabilizer has excellent oxidation resistance, thermal stability and storage stability.

Description

technical field [0001] The invention relates to a protein-EGCG composite nano particle and anti-oxidation Pickering high internal phase emulsion. Background technique [0002] Epigallocatechin gallate (EGCG) is a catechin monomer isolated from tea leaves, which has antibacterial, antiviral, antioxidative, antiarteriosclerotic, antithrombotic, antivascular It can prevent thrombosis, cancer, diabetes, cardiovascular disease, neurodegenerative diseases and other diseases. Therefore, it is an inherent demand for the development of the health industry to develop some functional foods using their excellent properties. However, EGCG has poor stability, is easily affected by temperature, pH and oxygen, and its oral bioavailability is very low, which also makes this kind of polyphenol active substances lack significant curative effect in clinical application. [0003] Studies have found that nano-embedding technology (that is, embedding active substances inside nanoparticles or ads...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/22A61K9/16A61K9/107A61K31/015A61K31/353A61P39/06A61P31/04A61P31/12A61P9/10A61P7/02A61P9/00A61P29/00A61P35/00A61P3/10A61P25/28A61K8/64A61K8/49A61K8/02A61K8/06A23L33/105A23L33/185
CPCA23V2002/00A61K8/022A61K8/06A61K8/498A61K8/64A61K9/107A61K9/1617A61K9/1658A61K31/015A61K31/353A61K47/22A61K47/42A61K2800/52A61K2800/522A23L33/105A23L33/185A23P10/20A61P3/10A61P7/02A61P9/00A61P9/10A61P25/28A61P29/00A61P31/04A61P31/12A61P35/00A61P39/06A61K2300/00A23V2200/30A23V2250/214A23V2250/5488
Inventor 唐传核彭丽萍
Owner SOUTH CHINA UNIV OF TECH
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