Enzymatic modification of saponins

Abstract

Methods for enzymatic modification of saponin produced by plant cell culture, products prepared thereby, uses of the products, and other related aspects, are disclosed. The saponin can be obtained from a plant cell culture extract of the species of the Quillaja, such as an extract of the Quillaja saponica Molina. The invention further relates to a method for preparing the saponin from the extract of the Quillaja saponica Molina.

Description

Technical Field

[0001] The application relates generally to saponin, especially for the method for the enzymatic modification of the saponin produced by in vitro plant cell culture, the saponin product prepared thus, the purposes and other related aspects of the saponin.The application further relates to use glucosidase and rhamnosidase, especially the glucosidase and rhamnosidase of sudden change, to increase the cell culture production of interested saponin.Saponin can be obtained from the plant cell that can synthesize saponin, particularly be derived from the plant cell of Quillaja species, comprise and can be obtained from its plant cell culture extract. Background Art

[0002] Saponins are steroid or terpenoid glycosides that have a wide range of uses, from fire extinguisher foams to food additives and immune boosters (Reichert, 2019).

[0003] Saponins have been of interest as immunostimulants for decades (see, for example, Hyslop, 1969). Traditionally, saponins have been purified from plants such as the soap tree (Quillaja saponaria Molina). Quil A is a saponin fraction derived from an aqueous extract of the bark of the soap tree (Dalsgaard, 1974). Quil A itself contains multiple components, of which the four most prominent Quil A fractions, purified by reverse-phase chromatography—QS-7, QS-17, QS-18, and QS-21—all exhibit immunostimulatory activity, despite varying hemolytic activity and toxicity (Kensil, 1991; Kensil, 1995). The major saponin fraction, QS-18, was found to be highly toxic to mice, while the saponin fractions QS-7 and QS-21 were much less toxic. QS-21 is more abundant than QS-7 and is the most extensively studied saponin adjuvant (Ragupathi, 2011).

[0004] Liquid chromatography / mass spectrometry analysis of aqueous / methanolic extracts of Quillaja saponaria bark revealed over 100 saponins, many of which have been structurally assigned (Nyberg, 2000; Nyberg, 2003; Kite, 2004).

[0005] A St.-Hil & Tul Mart. extract has been described, with the identities of the various components confirmed by mass spectrometry. Many of the saponin components in the Quillaja brasiliensis extract correspond to saponins found in Quillaja extract (Wallace, 2017; Wallace, 2019), and the Quillaja brasiliensis extract has also been shown to have immunostimulatory effects (Cibulski, 2018; Yendo, 2017).

[0006] Adjuvant System 01 (AS01) is a liposome-based adjuvant containing two immunostimulants, 3-O-deacylated-4'-monophosphoryl lipid A (3D-MPL) and QS-21 (Garcon, 2011; Didierlaurent, 2017). 3D-MPL is a non-toxic derivative of the lipopolysaccharide of Salmonella minnesota. AS01 is included in malaria vaccines (RTS, S-Mosquirix). TM ) and shingles vaccine (HZ / su-Shingrix TM ) and multiple vaccine candidates. AS01 injection leads to rapid and transient activation of innate immunity in animal models. Neutrophils and monocytes are rapidly recruited to draining lymph nodes (dLN) after immunization. In addition, AS01 induces MHCII 高 Recruitment and activation of dendritic cells (DCs), which are required for T cell activation (Didierlaurent, 2014). Some data are also available on the mechanism of action of AS01 components. 3D-MPL signals through TLR4, stimulating NF-κB transcriptional activity and cytokine production, and directly activates antigen-presenting cells (APCs) in humans and mice (De Becker, 2000; Ismaili, 2002; Martin, 2003; Mata-Haro, 2007). QS-21 promotes high antigen-specific antibody responses and CD8+ T cell responses in mice (Kensil, 1998; Newman, 1992; Soltysik, 1995) and antigen-specific antibody responses in humans (Livingston, 1994). Due to its physical properties, it is thought that QS-21 may act as a danger signal in vivo (Lambrecht, 2009; Li, 2008). Although QS-21 has been shown to activate the ASC-NLRP3 inflammasome and subsequent IL-1β / IL-18 release (Marty-Roix, 2016), the exact molecular pathways involved in the adjuvant effects of saponins remain to be elucidated.

[0007] Extracts of Quillaja saponaria are commercially available, including fractions of varying purity, such as Quil A, Fraction A, Fraction B, Fraction C, QS-7, QS-17, QS-18, and QS-21.

[0008] In the development of quality control methods to provide degraded reference samples, it has been described that Enzymatic hydrolysis of Quil-A by Revelation Aroma (Lecas, 2021).

[0009] The source of saponin mainly depends on natural resources at present, and this natural resource may be limited. As a substitute of Quillaja bark, a cell culture derivative method has been developed, and it is expected that a more sustainable saponin source will become. For example, WO 94 / 10291 discloses the cell of the culture of Quillaja and the method for preparing saponin, to be used as the active substance that can be used as an adjuvant. However, the interested saponin that produces like this may have a lower content. In addition, some saponin may be very troublesome to be separated with other components (especially other saponin components that may have similar structures). Therefore, there is still a need to improve the yield of the interested saponin that produces in the plant cell culture and / or promote the novel method of removing unwanted saponin components.

[0010] Modestobacter marinus glucosidase (Uniparc Ref. UPI000260A2FA, Uniprot Ref. I4EYD5) is a naturally occurring glucosidase.There remains a need for further glucosidases with improved properties.

[0011] Kribbella flavida rhamnosidase (Uniparc Ref. UPI00019BDB13, Uniprot Ref. D2PMT) is a naturally occurring rhamnosidase.There remains a need for further rhamnosidases with improved properties. Summary of the Invention

[0012] The present invention provides a method for preparing a saponin product, the method comprising the following steps:

[0013] (i) providing a plant cell culture extract comprising saponins; and

[0014] (ii) enzymatically converting a starting saponin from said plant cell culture extract into said product saponin.

[0015] A method for preparing a saponin product is also provided, the method comprising the following steps:

[0016] (i) cultivating a plant cell capable of synthesizing saponins under conditions that result in the synthesis of saponins;

[0017] (ii) recovering saponins from plant cell cultures; and

[0018] (iii) enzymatically converting the starting saponin from the recovered saponins into the product saponin.

[0019] A method for preparing a saponin product is also provided, the method comprising the following steps:

[0020] (i) cultivating plant cells capable of synthesizing saponins under conditions that result in the synthesis of saponins;

[0021] (ii) enzymatically converting a starting saponin from a synthesized saponin into said product saponin; and

[0022] (iii) Recovery of saponins from plant cell cultures.

[0023] Also provided is a method for increasing the amount of product saponin obtainable from a plant cell culture, the method comprising the steps of:

[0024] (i) cultivating plant cells capable of synthesizing saponins under conditions that result in the synthesis of saponins;

[0025] (ii) recovering saponins from plant cell cultures; and

[0026] (iii) enzymatically converting the starting saponin from the recovered saponins into the product saponin.

[0027] Also provided is a method for increasing the amount of product saponin obtainable from a plant cell culture, the method comprising the steps of:

[0028] (i) cultivating plant cells capable of synthesizing saponins under conditions that result in the synthesis of saponins;

[0029] (ii) enzymatically converting a starting saponin from a synthesized saponin into said product saponin; and

[0030] (iii) Recovery of saponins from plant cell cultures.

[0031] Also provided is a method for reducing the amount of starting saponins obtainable from a plant cell culture, the method comprising the steps of:

[0032] (i) cultivating plant cells capable of synthesizing saponins under conditions that result in the synthesis of saponins;

[0033] (ii) recovering saponins from plant cell cultures; and

[0034] (iii) enzymatically converting the starting saponin from the recovered saponins to the product saponin.

[0035] Also provided is a method for reducing the amount of starting saponins obtainable from a plant cell culture, the method comprising the steps of:

[0036] (i) cultivating plant cells capable of synthesizing saponins under conditions that result in the synthesis of saponins;

[0037] (ii) enzymatically converting a starting saponin from a synthesized saponin into said product saponin; and

[0038] (iii) Recovery of saponins from plant cell cultures.

[0039] Also provided is a method for producing saponins by plant cell culture, the method comprising the following steps:

[0040] (i) cultivating a plant cell capable of synthesizing saponins under conditions that result in the synthesis of saponins; and

[0041] (ii) recovering saponins from plant cell cultures,

[0042] The yield of the product saponin is increased by enzymatically converting the starting saponin from the recovered saponin to the product saponin.

[0043] The present invention also provides the use of glucosidase and / or rhamnosidase for enzymatic conversion of starting saponins obtained from plant cell culture into product saponins according to the method of the present invention.

[0044] Suitably, the glucosidase is an enzyme comprising, for example consisting of: (i) an enzyme according to SEQ ID or (ii) an amino acid sequence according to SEQ ID NO: 262, 208, 63, 229, 250, 5, 101, 207, 169, 247, 302, 324, 319, 9, 240, 325, 338, 850, 879, 868, 826, 804, 888, 881, 891, 816, 827, 857, 853, 842, 814, 886, 885, 838, 829, 808, 828, 870, 873, 844, 882, 874, 825, 824, 823, 810, 894, 849, 803, 890, 841, 832, 830, 845, 871, 837, 883 or 809, or a functional variant thereof; or 82, 874, 825, 824, 823, 810, 894, 849, 803, 890, 841, 832, 830, 845, 871, 837, 883 or 809, or a functional variant thereof.

[0045] Suitably, the rhamnosidase is an enzyme comprising, e.g. consisting of, an amino acid sequence according to SEQ ID No. 992, 1003, 1052, 1073, 1017, 1055, 1075, 1001, 1007, 1061, 1079, 1027, 1039, 1041, 989, 1053, 1018, 1066, 1082, 1076, 993, 1077, 1046, 1015, 1063, 1054, 1074, 1067 or 1033, or a functional variant thereof.

[0046] The present invention also provides the use of engineered glucosidase and / or engineered rhamnosidase polypeptides for enzymatically converting starting saponins obtained from plant cell culture into product saponins according to the method of the present invention.

[0047] Suitably, the engineered glucosidase polypeptide comprises, for example consists of, an amino acid sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% identity to the amino acid sequence of SEQ ID No. 262, or a functional fragment thereof, wherein the engineered glucosidase polypeptide comprises at least one residue substitution from:

[0048] F44Y;

[0049] V60L;

[0050] G117A;

[0051] F170N;

[0052] V263G or V263L;

[0053] N351H or N351Q;

[0054] A355H, A355I, A355L, A355M, A355R, A355T or A355W;

[0055] A356P;

[0056] R357A, R357C, R357K, R357M or R357Q;

[0057] G362C;

[0058] T365A, T365N, or T365S;

[0059] L367C;

[0060] V394R;

[0061] V395Y;

[0062] Q396E, Q396G, Q396N, Q396P, Q396R, Q396S, or Q396Y;

[0063] F430W;

[0064] R435F;

[0065] V438T;

[0066] V440F;

[0067] F442M or F442Q;

[0068] G444T;

[0069] A473F or A473R;

[0070] L474C, L474I, or L474V;

[0071] I475F;

[0072] L492C, L492G, L492H, L492I, L492N, L492Q, L492V, L492W, or L492Y;

[0073] Q493F or Q493H;

[0074] P494H or P494I;

[0075] S495I, S495K or S495Q;

[0076] G496P or G496W;

[0077] D498A, D498E, D498F, D498I, D498K, D498L, D498N, D498P, D498R, D498S, D498T or D498V;

[0078] A502R;

[0079] M504G or M504R;

[0080] L507A or L507R;

[0081] T508M;

[0082] L529M;

[0083] F535P;

[0084] A536D or A536E;

[0085] A537R;

[0086] F541A, F541I, F541L, F541M, or F541V;

[0087] L542I;

[0088] Q543G or Q543L;

[0089] E547L; and

[0090] Y585W.

[0091] Suitably, the engineered rhamnosidase polypeptide comprises, e.g., consists of, an amino acid sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% identity to the amino acid sequence of SEQ ID No. 1017, or a functional fragment thereof, wherein the engineered rhamnosidase polypeptide comprises at least one residue substitution from:

[0092] (i)A56C

[0093] (ii)A143P

[0094] (iii) Q181H, Q181R or Q181S

[0095] (iv)L214M

[0096] (v)G215S

[0097] (vi)F216M

[0098] (vii) G218D or G218N

[0099] (viii)K219G

[0100] (ix)A238M

[0101] (x)T252Y

[0102] (xi)T311W

[0103] (xii)V326C

[0104] (xiii)G357C

[0105] (xiv) S369C, S369I, S369K or S369M

[0106] (xv)I487M, I487Q, or I487V

[0107] (xvi)K492N

[0108] (xvii)V499T

[0109] (xviii)G508S

[0110] (xix)R543C

[0111] (xx)L557Y

[0112] (xxi)G634A

[0113] (xxii)S635N

[0114] (xxiii)A690C and

[0115] (xxiv)Q921H.

[0116] The present invention also provides saponins prepared by the methods herein, saponin-containing compositions comprising the saponins prepared by the methods herein, adjuvant compositions comprising the saponins or saponin-containing compositions, and adjuvant compositions prepared using the saponins or saponin-containing compositions.

[0117] Also provided is a method for preparing an adjuvant, comprising the following steps: preparing saponin or a composition containing saponin from a plant cell culture extract according to the method of the present invention, and formulating the saponin or the composition containing saponin into an adjuvant.

[0118] Also provided is the use of a saponin obtained from the plant cell culture extract of the present invention or a composition containing saponin for preparing an adjuvant composition.

[0119] Also provided are immunogenic compositions comprising a saponin or a saponin-containing composition according to the invention and an antigen or a polynucleotide encoding the antigen.

[0120] Also provided is a method for preparing an immunogenic composition, comprising the steps of preparing an adjuvant according to the method of the present invention and formulating it into a composition comprising an antigen or a polynucleotide encoding the antigen.

[0121] Also provided are kits comprising (i) a saponin or a composition containing a saponin according to the present invention and (ii) an antigen or a polynucleotide encoding the antigen. BRIEF DESCRIPTION OF THE DRAWINGS

[0122] Figure 1 HPLC chromatogram of crude aqueous extract of Quillaja sapodilla bark

[0123] Figure 2 HPLC-UV chromatogram of crude aqueous extract of Quillaja saponaria bark

[0124] Figure 3 UPLC-UV chromatogram of crude aqueous extract of Quillaja sapodilla bark

[0125] Figure 4UPLC-UV chromatogram of polystyrene-purified saponin extract of Quillaja saponaria QS-21 with low content of 2018 component

[0126] Figure 5 UPLC-UV / MS chromatogram of the purified saponin extract of Quillaja saponaria QS-21 with low content of 2018 component

[0127] Figure 6 Detail of the UPLC-UV / MS chromatogram of the purified saponin extract of Quillaja saponaria QS-21 with low levels of the 2018 component.

[0128] Figure 7 A-7B 1988 ( Figure 7 A) and 2002( Figure 7 B) Mass spectrum of extracted molecular weight ions

[0129] Figure 8 Combined centroid profile of purified saponin extracts from Quillaja saponaria QS-21 with low content of 2018 components

[0130] Figure 9 UPLC-UV chromatogram of the purified saponin extract of Quillaja saponaria QS-21 with low 2018 components

[0131] Figure 10 LCMS / MS chromatograms of the content of QS-18 2150 (panel A) and QS-21 1988 (panel B) components in the QS-21 standard in Example 4-2

[0132] Figure 11 LCMS / MS chromatograms of the component contents of QS-18 2150 (panel A) and QS-21 1988 (panel B) after negative control treatment in Example 4-2

[0133] Figure 12 LCMS / MS chromatograms of the component contents of QS-18 2150 (panel A) and QS-211988 (panel B) after treatment with glucosidase SEQ ID No. 262 in Example 4-2

[0134] Figure 13 UV HPLC chromatograms of crude bark extract (CBE) in Example 4-4 after treatment with glucosidase SEQ ID No. 262 (upper trace) and negative control treatment (lower trace)

[0135] Figure 14 LCMS / MS chromatograms of the component contents of QS-17 2296 (panel A) and QS-18 2150 (panel B) after negative control treatment in Example 6-1

[0136] Figure 15 LCMS / MS chromatograms of the deglucosyl-QS-17 2134 (panel A) and QS-21 1988 (panel B) components after negative control treatment in Example 6-1

[0137] Figure 16 LCMS / MS chromatograms of the component contents of QS-17 2310 (panel A) and QS-18 2164 (panel B) after negative control treatment in Example 6-1

[0138] Figure 17 LCMS / MS chromatograms of the component contents of QS-17 2296 (panel A) and QS-18 2150 (panel B) after treatment with rhamnosidase SEQ ID No. 1017 in Example 6-1

[0139] Figure 18 LCMS / MS chromatograms of the deglucosylation components QS-17 2134 (panel A) and QS-21 1988 (panel B) after treatment with rhamnosidase SEQ ID No. 1017 in Example 6-1

[0140] Figure 19 LCMS / MS chromatograms of the component contents of QS-17 2310 (panel A) and QS-18 2164 (panel B) after treatment with rhamnosidase SEQ ID No. 1017 in Example 6-1

[0141] Figure 20 UV HPLC chromatograms of the treated bark extract (TBE) in Example 6-2 after treatment with rhamnosidase SEQ ID No. 1017 (upper trace) and negative control treatment (lower trace)

[0142] Figure 21 UV HPLC chromatograms of CBE treated with rhamnosidase SEQ ID No. 1017 (upper trace) and negative control (lower trace) in Example 6-3

[0143] Figure 22 LCMS / MS chromatograms of the QS-21 1988 component content at T0 (panel A) and 24 hours (panel B) after dual enzyme treatment of CBE in Example 7

[0144] Figure 23A-B Glucosidase treatment of CBE in Example 8 ( Figure 23a ) and the negative control treatment of CBE ( Figure 23b ) after the exemplary UV HPLC chromatogram

[0145] Figure 24Exemplary UV HPLC chromatograms after rhamnosidase treatment of CBE (upper trace) and negative control treatment of CBE (lower trace) in Example 9

[0146] Figure 25 1 L scale HPLC-UV chromatograms of untreated and enzyme-treated CBE from Example 11

[0147] Figure 26 UPLC-UV chromatograms of untreated and enzyme-treated CBE from Example 11 after 1 L scale purification (full acquisition)

[0148] Figure 27 UPLC-UV chromatograms of untreated and enzyme-treated CBE from Example 11 after 1 L scale purification (zoomed)

[0149] Figure 28 Saponin productivity from the untreated and double enzyme treated plant cell cultures of Example 12. The content of the saponin components shown in the crude cell extract (CCE) obtained from the plant cell cultures was analyzed and measured by LCMS / MS. The corresponding saponin component concentrations (expressed as ug / ml culture) were calculated retrospectively relative to the plant cell cultures. For ease of visualization, the concentration of each analyzed saponin component has been regrouped in a bar in the bar graph. The identity of the analyzed saponin components is represented by the color code provided in the figure.

[0150] Figure 29 Saponin profile and ratios in plant cell culture extracts. The indicated saponin components were analyzed and measured by LCMS / MS at the indicated steps of the method described in Example 13: (i) before enzyme treatment; (ii) after enzyme treatment; (iii) after treatment - UF / DF concentration; and (iv) after treatment - phenyl chromatography. Results are expressed as the percentage of each saponin component compared to the sum of the percentages of all saponin components analyzed (total 100%). The identity of the analyzed saponin components is indicated by the color code provided in the figure.

[0151] A brief description of sequence identity

[0152] Seq ID No. 1: Amino acid sequence of Cyberlindnera fabianii glucosidase, Uniparc reference UPI00049B1A8C, Uniprot reference A0A061B3J2.

[0153] Seq ID No. 2: Amino acid sequence of the following: Flavobacterium gilvum glucosidase, Uniparc reference UPI0004E3EF7B, Uniprot reference A0A085EII0.

[0154] Seq ID No. 3: Amino acid sequence of the following: Algibacter lectus glucosidase, Uniparc reference UPI00050EE490, Uniprot reference A0A090X649.

[0155] Seq ID No. 4: Amino acid sequence of the following: Microbacterium azadirachtae glucosidase, Uniparc reference UPI0005ECB51E, Uniprot reference A0A0F0LB94.

[0156] Seq ID No. 5: Amino acid sequence of the following: Actinobacteria bacterium glucosidase, Uniparc reference UPI0006588DAD, Uniprot reference A0A0JOUT37.

[0157] Seq ID No. 6: Amino acid sequence of the following: Chloroflexi bacterium glucosidase, Uniparc reference UPI0007968552, Uniprot reference A0A136KWB3.

[0158] Seq ID No. 7: Amino acid sequence of the following: Komagataeibacter rhaeticus glucosidase, Uniparc reference UPI0002080410, Uniprot reference A0A181C809.

[0159] Seq ID No. 8: Amino acid sequence of: Bacteroides sp. glucosidase, Uniparc reference UPI0008211BFC, Uniprot reference A0A1C5WEL8.

[0160] Seq ID No. 9: Amino acid sequence of the following: Streptomyces rubrolavendulae glucosidase, Uniparc reference UPI00085A2BD0, Uniprot reference A0A1D8FZW3.

[0161] Seq ID No. 10: Amino acid sequence of the following: Streptomyces rubrolavendulae glucosidase, Uniparc reference UPI00098C60F6, Uniprot reference A0A1S8KYM5.

[0162] Seq ID No. 11: Amino acid sequence of the following: Uncultured bacterial glucosidase, Uniparc reference UPI0009CE0D4C, Uniprot reference A0A1V5M6V6.

[0163] Seq ID No. 12: Amino acid sequence of the following: Firmicutes bacterium glucosidase, Uniparc reference UPI0009D4127D, Uniprot reference A0A1V6AN95.

[0164] Seq ID No. 13: Amino acid sequence of the following: Anthracocystis flocculosa glucosidase, Uniparc reference UPI00045601AB, Uniprot reference A0A061H1Z3.

[0165] Seq ID No. 14: Amino acid sequence of the following: Bifidobacterium boum glucosidase, Uniparc reference UPI0004FF77C7, Uniprot reference A0A086ZKU2.

[0166] Seq ID No. 15: Amino acid sequence of the following: Jejuia pallidilutea glucosidase, Uniparc reference UPI00051EDBDE, Uniprot reference A0A098LTR2.

[0167] Seq ID No. 16: Amino acid sequence of the following: Ceratocystis fimbriata glucosidase, Uniparc reference UPI00062105AB, Uniprot reference A0A0F8B2B0.

[0168] Seq ID No. 17: Amino acid sequence of the following: Actinobacteria bacterium glucosidase, Uniparc reference UPI0006583AB1, Uniprot reference A0A0J0UVW7.

[0169] Seq ID No. 18: Amino acid sequence of: Rhodococcus sp. glucosidase, Uniparc reference UPI0007AABFAD, Uniprot reference A0A143QAX3.

[0170] Seq ID No. 19: Amino acid sequence of the following: Valsa mali glucosidase, Uniparc reference UPI0007F2D02D, Uniprot reference A0A194VF47.

[0171] Seq ID No. 20: Amino acid sequence of: Uncultured Bacteroides sp. glucosidase, Uniparc reference UPI000821004D, Uniprot reference A0A1C5WSI4.

[0172] Seq ID No. 21: Amino acid sequence of the following: Eisenbergiella tayi glucosidase, Uniparc reference UPI00084089B2, Uniprot reference A0A1E3ALT2.

[0173] Seq ID No. 22: Amino acid sequence of Streptomyces sp. glucosidase, Uniparc reference UPI000978E914, Uniprot reference A0A1V2MYI4.

[0174] Seq ID No. 23: Amino acid sequence of the following: Firmicutes bacterium glucosidase, Uniparc reference UPI0009CBF21C, Uniprot reference A0A1V5MH90.

[0175] Seq ID No. 24: Amino acid sequence of the following: Tenericutes bacterium glucosidase, Uniparc reference UPI0009D5B1F0, Uniprot reference A0A1V6BAK3.

[0176] Seq ID No. 25: Amino acid sequence of the following: Gluconobacter oxydans glucosidase, Uniparc reference UPI0004A87350, Uniprot reference A0A067Z479.

[0177] Seq ID No. 26: Amino acid sequence of the following: Bifidobacterium catenulatum glucosidase, Uniparc reference UPI00050787A2, Uniprot reference A0A087B8Q8.

[0178] Seq ID No. 27: Amino acid sequence of: Bionectria ochroleuca glucosidase, Uniparc reference UPI0005965863, Uniprot reference A0A0B7K538.

[0179] Seq ID No. 28: Amino acid sequence of Parcubacteria sp. glucosidase, Uniparc reference UPI0006377CA4, Uniprot reference A0A0G0GD78.

[0180] Seq ID No. 29: Amino acid sequence of the following: Microbacterium ketosireducens glucosidase, Uniparc reference UPI0006228575, Uniprot reference A0A0M2H276.

[0181] Seq ID No. 30: Amino acid sequence of Roseburia faecis glucosidase, Uniparc reference UPI0006C454B4, Uniprot reference A0A173R3W4.

[0182] Seq ID No. 31: Amino acid sequence of the following: Kwoniella dejecticola glucosidase, Uniparc reference UPI0007F1D695, Uniprot reference A0A1A6A050.

[0183] Seq ID No. 32: Amino acid sequence of: Uncultured Clostridium sp. glucosidase, Uniparc reference UPI0008232A70, Uniprot reference A0A1C6C862.

[0184] Seq ID No. 33: Amino acid sequence of the following: Cyberlindnera jadinii glucosidase, Uniparc reference UPI000866CB30, Uniprot reference A0A1E4S2F8.

[0185] Seq ID No. 34: Amino acid sequence of the following: Bacteroidetes bacterium glucosidase, Uniparc reference UPI0009D3483D, Uniprot reference A0A1V5G4W6.

[0186] Seq ID No. 35: Amino acid sequence of the following: Verrucomicrobia bacterium glucosidase, Uniparc reference UPI0009CC9AEA, Uniprot reference A0A1V5Q4R4.

[0187] Seq ID No. 36: Amino acid sequence of the following: Bacteroidetes bacterium glucosidase, Uniparc reference UPI0009C6DE73, Uniprot reference A0A1V6BV25.

[0188] Seq ID No. 37: Amino acid sequence of the following: Lichtheimia ramosa glucosidase, Uniparc reference UPI0004E051A9, Uniprot reference A0A077WUK7.

[0189] Seq ID No. 38: Amino acid sequence of the following: Bifidobacterium mongoliense glucosidase, Uniparc reference UPI0005060F52, Uniprot reference A0A087BWT7.

[0190] Seq ID No. 39: Amino acid sequence of Vibrio ishigakensis glucosidase, Uniparc reference UPI000591CED9, Uniprot reference A0A0B8NZY1.

[0191] Seq ID No. 40: Amino acid sequence of: Phaeomoniella chlamydospora glucosidase, Uniparc reference UPI00063B706F, Uniprot reference A0A0G2HEV5.

[0192] Seq ID No. 41: Amino acid sequence of the following: Ardenticatena maritima glucosidase, Uniparc reference UPI0006C04F59, Uniprot reference A0A0M8K5H7.

[0193] Seq ID No. 42: Amino acid sequence of: Coprococcus commensal glucosidase, Uniparc reference UPI000197E031, Uniprot reference A0A173WPC4.

[0194] Seq ID No. 43: Amino acid sequence of the following: Nocardioides dokdonensis glucosidase, Uniparc reference UPI0007DDBAB3, Uniprot reference A0A1A9GNJ0.

[0195] Seq ID No. 44: Amino acid sequence of uncultured Clostridium sp. glucosidase, Uniparc reference UPI000822F7EB, Uniprot reference A0A1C6EBC1.

[0196] Seq ID No. 45: Amino acid sequence of the following: Acetobacterium wieringae glucosidase, Uniparc reference UPI000878EB40, Uniprot reference A0A1F2PFB4.

[0197] Seq ID No. 46: Amino acid sequence of the following: Tenericutes bacterium glucosidase, Uniparc reference UPI0009D0A2C1, Uniprot reference A0A1V5HNJ3.

[0198] Seq ID No. 47: Amino acid sequence of the following: Tenericutes bacterium glucosidase, Uniparc reference UPI0009CB8A73, Uniprot reference A0A1V5UFI1.

[0199] Seq ID No. 48: Amino acid sequence of the following: Uncultured bacterial glucosidase, Uniparc reference UPI0009CC90AF, Uniprot reference A0A1V6CDT2.

[0200] Seq ID No. 49: Amino acid sequence of Parabacteroides distasonis glucosidase, Uniparc reference UPI0004D8E473, Uniprot reference A0A078SYD0.

[0201] Seq ID No. 50: Amino acid sequence of Bifidobacterium psychraerophilum glucosidase, Uniparc reference UPI0005006C6A, Uniprot reference A0A087CJB0.

[0202] Seq ID No. 51: Amino acid sequence of: Hebeloma cylindrosporum glucosidase, Uniparc reference UPI00059A3BA8, Uniprot reference A0A0C2YJF2.

[0203] Seq ID No. 52: Amino acid sequence of the following: Brenneria goodwinii glucosidase, Uniparc reference UPI0006579FEA, Uniprot reference A0A0G4JRR8.

[0204] Seq ID No. 53: Amino acid sequence of: Aspergillus calidoustus glucosidase, Uniparc reference UPI00073C9E60, Uniprot reference A0A0U5GN20.

[0205] Seq ID No. 54: Amino acid sequence of the following: Bacteroides finegoldii glucosidase, Uniparc reference UPI0006C6E0C3, Uniprot reference A0A174BEZ2.

[0206] Seq ID No. 55: Amino acid sequence of the following: Altererythrobacter dongtanensis glucosidase, Uniparc reference UPI0008153D6D, Uniprot reference A0A1B2A943.

[0207] Seq ID No. 56: Amino acid sequence of the following: Uncultured Anaerotruncus sp. glucosidase, Uniparc reference UPI0008206E7B, Uniprot reference A0A1C6FWD9.

[0208] Seq ID No. 57: Amino acid sequence of the following: Candidatus firestone glucosidase, Uniparc reference UPI0008AC0E17, Uniprot reference A0A1F5UJR6.

[0209] Seq ID No. 58: Amino acid sequence of the following: Tenericutes bacterium glucosidase, Uniparc reference UPI0009C6C8E4, Uniprot reference A0A1V5HS22.

[0210] Seq ID No. 59: Amino acid sequence of the following: Lentisphaeraebacterium glucosidase, Uniparc reference UPI0009CEE1AB, Uniprot reference A0A1V5VBL2.

[0211] Seq ID No. 60: Amino acid sequence of the following: Planctomycetes bacterium glucosidase, Uniparc reference UPI0009CAAB0A, Uniprot reference A0A1V6FZ47.

[0212] Seq ID No. 61: Amino acid sequence of: Pseudallescheria apiosperma glucosidase, Uniparc reference UPI0004DD62AC, Uniprot reference A0A084G332.

[0213] Seq ID No. 62: Amino acid sequence of the following: Nonlabens sediminis glucosidase, Uniparc reference UPI000507F00A, Uniprot reference A0A090Q4N8.

[0214] Seq ID No. 63: Amino acid sequence of the following: Gynuella sunshinyii glucosidase, Uniparc reference UPI0005CC42CA, Uniprot reference A0A0C5VDU3.

[0215] Seq ID No. 64: Amino acid sequence of the following: Verticillium longisporum glucosidase, Uniparc reference UPI00063E4005, Uniprot reference A0A0G4N9Q7.

[0216] Seq ID No. 65: Amino acid sequence of the following: Cellulomonas sp. glucosidase, Uniparc reference UPI00073C6CD3, Uniprot reference A0A0V8TAB5.

[0217] Seq ID No. 66: Amino acid sequence of the following: Hungatella hathewayi glucosidase, Uniparc reference UPI0006C024BB, Uniprot reference A0A174FBX7.

[0218] Seq ID No. 67: Amino acid sequence of: Mesorhizobium sp. glucosidase, Uniparc reference UPI000688E74D, Uniprot reference A0A1C2DG64.

[0219] Seq ID No. 68: Amino acid sequence of Clostridium sp. glucosidase, Uniparc reference UPI000822FAB7, Uniprot reference A0A1C6GRT5.

[0220] Seq ID No. 69: Amino acid sequence of the following: Chlamydiales bacterium glucosidase, Uniparc reference UPI0009284E74, Uniprot reference A0A1M3CSY6.

[0221] Seq ID No. 70: Amino acid sequence of the following: Spirochaetes bacterium glucosidase, Uniparc reference UPI0009CB461D, Uniprot reference A0A1V5HUX3.

[0222] Seq ID No. 71: Amino acid sequence of the following: bacterial glucosidase, Uniparc reference UPI0009C92D6A, Uniprot reference A0A1V5VHB9.

[0223] Seq ID No. 72: Amino acid sequence of the following: Thermotogae bacterium glucosidase, Uniparc reference UPI0009D12B86, Uniprot reference A0A1V6H2W5.

[0224] Seq ID No. 73: Amino acid sequence of: Pseudallescheria apiosperma glucosidase, Uniparc reference UPI0004DCBED3, Uniprot reference A0A084GGE2.

[0225] Seq ID No. 74: Amino acid sequence of the following: Algibacter lectus glucosidase, Uniparc reference UPI00050E0BE3, Uniprot reference A0A090VF17.

[0226] Seq ID No. 75: Amino acid sequence of the following: Paxillus involutus glucosidase, Uniparc reference UPI0005B075C8, Uniprot reference A0A0C9TWP5.

[0227] Seq ID No. 76: Amino acid sequence of the following: Verticillium longisporum glucosidase, Uniparc reference UPI00063DF006, Uniprot reference A0A0G4NA55.

[0228] Seq ID No. 77: Amino acid sequence of the following: Mucilaginibacter gotjawali glucosidase, Uniparc reference UPI00076F8EA4, Uniprot reference A0A110B1H1.

[0229] Seq ID No. 78: Amino acid sequence of the following: Bacteroides uniformis glucosidase, Uniparc reference UPI0006C0FFEF, Uniprot reference A0A174IWW4.

[0230] Seq ID No. 79: Amino acid sequence of Coprococcus sp. glucosidase, Uniparc reference UPI0008222B77, Uniprot reference A0A1C5W0N6.

[0231] Seq ID No. 80: Amino acid sequence of Blautia sp. glucosidase, Uniparc reference UPI0006C36823, Uniprot reference A0A1C6K2X5.

[0232] Seq ID No. 81: Amino acid sequence of Cellulomonas sp. glucosidase, Uniparc reference UPI00092B3CD4, Uniprot reference A0A1M3ELH4.

[0233] Seq ID No. 82: Amino acid sequence of the following: Bacterial glucosidase, Uniparc reference UPI0009C85428, Uniprot reference A0A1V5J984.

[0234] Seq ID No. 83: Amino acid sequence of the following: Spirochaetes bacterium glucosidase, Uniparc reference UPI0009CA1D5C, Uniprot reference A0A1V5WHQ7.

[0235] Seq ID No. 84: Amino acid sequence of the following: Tenericutes bacterium glucosidase, Uniparc reference UPI0009C7041F, Uniprot reference A0A1V6IJ57.

[0236] Seq ID No. 85: Amino acid sequence of the following: Flavobacterium gilvum glucosidase, Uniparc reference UPI0004E2A41F, Uniprot reference A0A085EG29.

[0237] Seq ID No. 86: Amino acid sequence of the following: Algibacter lectus glucosidase, Uniparc reference UPI0005102470, Uniprot reference A0A090WWZ1.

[0238] Seq ID No. 87: Amino acid sequence of the following: Hydnomerulius pinastri glucosidase, Uniparc reference UPI0005B0F0AE, Uniprot reference A0A0C9WDY0.

[0239] Seq ID No. 88: Amino acid sequence of the following: Nocardia farcinica glucosidase, Uniparc reference UPI00065C2666, Uniprot reference A0A0H5NWN2.

[0240] Seq ID No. 89: Amino acid sequence of: Bacteroides cellulosilyticus glucosidase, Uniparc reference UPI000760375B, Uniprot reference A0A125MG18.

[0241] Seq ID No. 90: Amino acid sequence of the following: Fonsecaea erecta glucosidase, Uniparc reference UPI0007DF4250, Uniprot reference A0A178ZBN2.

[0242] Seq ID No. 91: Amino acid sequence of Bacteroides sp. glucosidase, Uniparc reference UPI0008209852, Uniprot reference A0A1C5W9N9.

[0243] Seq ID No. 92: Amino acid sequence of the following: Tannerella forsythia glucosidase, Uniparc reference UPI00086C34C1, Uniprot reference A0A1D3UGH8.

[0244] Seq ID No. 93: Amino acid sequence of the following: Microbacterium esteraromaticum glucosidase, Uniparc reference UPI00097E83BB, Uniprot reference A0A1R4KI92.

[0245] Seq ID No. 94: Amino acid sequence of the following: bacterial glucosidase, Uniparc reference UPI0009D255E5, Uniprot reference A0A1V5LJK9.

[0246] Seq ID No. 95: Amino acid sequence of the following: Candidatus hydrogenedentes glucosidase, Uniparc reference UPI0009C5A3CF, Uniprot reference A0A1V5Z2L2.

[0247] Seq ID No. 96: Amino acid sequence of the following: Bacteroidetes bacterium glucosidase, Uniparc reference UPI0009C55799, Uniprot reference A0A1V6J4J8.

[0248] Seq ID No. 97: Amino acid sequence of the following: Penicillium solitum glucosidase, Uniparc reference UPI0009D4067F, Uniprot reference A0A1V6RQ41.

[0249] Seq ID No. 98: Amino acid sequence of the following: Weissella soli glucosidase, Uniparc reference UPI0008737AA2, Uniprot reference A0A288Q8I2.

[0250] Seq ID No. 99: Amino acid sequence of the following: Acetatifactor muris glucosidase, Uniparc reference UPI000CAC57D4, Uniprot reference A0A2K4ZN91.

[0251] Seq ID No. 100: Amino acid sequence of the following: Corynespora cassiicola glucosidase, Uniparc reference UPI000D237A4A, Uniprot reference A0A2T2NYD4.

[0252] Seq ID No. 101: Amino acid sequence of the following: Meira miltonrushii glucosidase, Uniparc reference UPI000D77C91D, Uniprot reference A0A316V6M3.

[0253] Seq ID No. 102: Amino acid sequence of the following: Bacteroides fragilis glucosidase, Uniparc reference UPI00004E1F76, Uniprot reference A0A380YVC7.

[0254] Seq ID No. 103: Amino acid sequence of: Malassezia restricta glucosidase, Uniparc reference UPI000F0C30E8, Uniprot reference A0A3G2SB79.

[0255] Seq ID No. 104: Amino acid sequence of the following: Fusarium euwallaceae glucosidase, Uniparc reference UPI000FFFEFB2, Uniprot reference A0A430LYA2.

[0256] Seq ID No. 105: Amino acid sequence of Psathyrella aberdarensis glucosidase, Uniparc reference UPI0010251887, Uniprot reference A0A4Q2E070.

[0257] Seq ID No. 106: Amino acid sequence of Aeromonas hydrophila glucosidase, Uniparc reference UPI0000E69509, Uniprot reference A0KLP6.

[0258] Seq ID No. 107: Amino acid sequence of the following: Saccharopolyspora erythraea glucosidase, Uniparc reference UPI00000B86CB, Uniprot reference A4F7P9.

[0259] Seq ID No. 108: Amino acid sequence of Streptomyces sviceus glucosidase, Uniparc reference UPI000180240E, Uniprot reference B51181.

[0260] Seq ID No. 109: Amino acid sequence of the following: Naematelia encephala glucosidase, Uniparc reference UPI000A250F78, Uniprot reference A0A1Y2AWB7.

[0261] Seq ID No. 110: Amino acid sequence of the following: Hartmannibacter diazotrophicus glucosidase, Uniparc reference UPI000C0221F1, Uniprot reference A0A2C9D6I2.

[0262] Seq ID No. 111: Amino acid sequence of the following: Pontimonas salivibrio glucosidase, Uniparc reference UPI000CEB5AB1, Uniprot reference A0A2L2BPE2.

[0263] Seq ID No. 112: Amino acid sequence of the following: Cadophora sp. glucosidase, Uniparc reference UPI000D5B9C38, Uniprot reference A0A2V1CH24.

[0264] Seq ID No. 113: Amino acid sequence of the following: Meira miltonrushii glucosidase, Uniparc reference UPI000D779558, Uniprot reference A0A316V8S0.

[0265] Seq ID No. 114: Amino acid sequence of the following: Monilinia fructigena glucosidase, Uniparc reference UPI000DC42E3D, Uniprot reference A0A395IJW4.

[0266] Seq ID No. 115: Amino acid sequence of the following: Hortaea werneckii glucosidase, Uniparc reference UPI000F3E476C, Uniprot reference A0A3M6XGS0.

[0267] Seq ID No. 116: Amino acid sequence of the following: Streptomyces netropsis glucosidase, Uniparc reference UPI00101460D7, Uniprot reference A0A445N7U0.

[0268] Seq ID No. 117: Amino acid sequence of the following: Aureobasidium pullulans glucosidase, Uniparc reference UPI001139C6A8, Uniprot reference A0A4S9IFI0.

[0269] Seq ID No. 118: Amino acid sequence of the following: Aspergillus clavatus glucosidase, Uniparc reference UPI0000EA5CFF, Uniprot reference A1CTN9.

[0270] Seq ID No. 119: Amino acid sequence of the following: Clavibacter michiganensis glucosidase, Uniparc reference UPI0001523037, Uniprot reference A5CT94.

[0271] Seq ID No. 120: Amino acid sequence of Penicillium rubens glucosidase, Uniparc reference UPI0001831CF5, Uniprot reference B6H7R5.

[0272] Seq ID No. 121: Amino acid sequence of the following: Lachnoclostridium sp. glucosidase, Uniparc reference UPI000B365547, Uniprot reference A0A1Y4NTL9.

[0273] Seq ID No. 122: Amino acid sequence of the following: Rhodobacteraceaebacterium glucosidase, Uniparc reference UPI000C09BF88, Uniprot reference A0A2D5IXB9.

[0274] Seq ID No. 123: Amino acid sequence of the following: Bacteroides fragilis glucosidase, Uniparc reference UPI0004B5EEF2, Uniprot reference A0A2M9UUC4.

[0275] Seq ID No. 124: Amino acid sequence of the following: Aspergillus indologenus glucosidase, Uniparc reference UPI000D7FE1ED, Uniprot reference A0A2V5IY78.

[0276] Seq ID No. 125: Amino acid sequence of Acaromyces ingoldii glucosidase, Uniparc reference UPI000D802B25, Uniprot reference A0A316YR39.

[0277] Seq ID No. 126: Amino acid sequence of the following: Monilinia fructigena glucosidase, Uniparc reference UPI000DC60823, Uniprot reference A0A395J1U5.

[0278] Seq ID No. 127: Amino acid sequence of the following: Paenibacillus xylanexedens glucosidase, Uniparc reference UPI000F52D773, Uniprot reference A0A3N6CA02.

[0279] Seq Id No. 128: Amino acid sequence of the following: Actinomyces howellii glucosidase, Uniparc reference UPI000F6DAAAE, Uniprot reference A0A448HIGO.

[0280] Seq ID No. 129: Amino acid sequence of: Friedmanniomyces endolithicus glucosidase, Uniparc reference UPI00113D19DF, Uniprot reference A0A4V5N9I4.

[0281] Seq ID No. 130: Amino acid sequence of Neosartorya fischeri glucosidase, Uniparc reference UPI0000EA8672, Uniprot reference A1DNS0.

[0282] Seq ID No. 131: Amino acid sequence of Pseudomonas aeruginosa glucosidase, Uniparc reference UPI0000D7314B, Uniprot reference A6V4K6.

[0283] Seq ID No. 132: Amino acid sequence of the following: Talaromyces stipitatus glucosidase, Uniparc reference UPI00018E7266, Uniprot reference B8MF24.

[0284] Seq ID No. 133: Amino acid sequence of the following: Aquimixticola soesokkakensis glucosidase, Uniparc reference UPI000A1A5FD7, Uniprot reference A0A1Y5RVF8.

[0285] Seq ID No. 134: Amino acid sequence of the following: Rhodobacterales bacterium glucosidase, Uniparc reference UPI000C98D37C, Uniprot reference A0A2D9YGV1.

[0286] Seq ID No. 135: Amino acid sequence of the following: Methylorubrum extorquens glucosidase, Uniparc reference UPI0006F9793E, Uniprot reference A0A2N9AS40.

[0287] Seq ID No. 136: Amino acid sequence of: Clostridium perfringens glucosidase, Uniparc reference UPI00000CF7C8, Uniprot reference A0A2X2YBP0.

[0288] Seq ID No. 137: Amino acid sequence of the following: Acholeplasmatales bacterium glucosidase, Uniparc reference UPI0008AEBEA3, Uniprot reference A0A348NID6.

[0289] Seq ID No. 138: Amino acid sequence of the following: Bacteroidetes bacterium glucosidase, Uniparc reference UPI000EC3C979, Uniprot reference A0A3B8VHE8.

[0290] Seq ID No. 139: Amino acid sequence of the following: Clostridium carnis glucosidase, Uniparc reference UPI000F637E38, Uniprot reference A0A3P6K8E8.

[0291] Seq ID No. 140: Amino acid sequence of the following: Mycolicibacterium flavescens glucosidase, Uniparc reference UPI000B93B5C9, Uniprot reference A0A448HNB5.

[0292] Seq ID No. 141: Amino acid sequence of: Streptococcus gallolyticus glucosidase, Uniparc reference UPI000F6EFA71, Uniprot reference A0A4V6LJ94.

[0293] Seq ID No. 142: Amino acid sequence of the following: Yersinia enterocolitica glucosidase, Uniparc reference UPI0000EB54CC, Uniprot reference A1JNB7.

[0294] Seq ID No. 143: Amino acid sequence of Anaeromyxobacter sp. glucosidase, Uniparc reference UPI0000ED8A80, Uniprot reference A7HFG4.

[0295] Seq ID No. 144: Amino acid sequence of the following: Talaromyces stipitatus glucosidase, Uniparc reference UPI00018E7D70, Uniprot reference B8MK55.

[0296] Seq ID No. 145: Amino acid sequence of the following: Hortaea werneckii glucosidase, Uniparc reference UPI000A2E3FAA, Uniprot reference A0A1Z5SL14.

[0297] Seq ID No. 146: Amino acid sequence of the following: Micavibrio sp. glucosidase, Uniparc reference UPI000C529025, Uniprot reference A0A2E2Q8X2.

[0298] Seq ID No. 147: Amino acid sequence of the following: Acidobacteriia bacterial glucosidase, Uniparc reference UPI000CE6B996, Uniprot reference A0A2N9MBS0.

[0299] Seq ID No. 148: Amino acid sequence of the following: Corynebacterium jeikeium glucosidase, Uniparc reference UPI000DA3A972, Uniprot reference A0A2X4T570.

[0300] Seq ID No. 149: Amino acid sequence of the following: Clostridiaceae bacterium glucosidase, Uniparc reference UPI000E8D37A1, Uniprot reference A0A353PZH8.

[0301] Seq ID No. 150: Amino acid sequence of the following: Anaerolineaceaebacterium glucosidase, Uniparc reference UPI000748C096, Uniprot reference A0A3B9PA35.

[0302] Seq ID No. 151: Amino acid sequence of the following: Gymnopilus dilepis glucosidase, Uniparc reference UPI000FF41956, Uniprot reference A0A409WSY0.

[0303] Seq ID No. 152: Amino acid sequence of the following: Kocuria rosea glucosidase, Uniparc reference UPI000F7105D4, Uniprot reference A0A448R8N0.

[0304] Seq ID No. 153: Amino acid sequence of the following: Teredinibacter sp. glucosidase, Uniparc reference UPI0011696FAB, Uniprot reference A0A509DWZ3.

[0305] Seq ID No. 154: Amino acid sequence of the following: Aspergillus niger glucosidase, Uniparc reference UPI0000EFB564, Uniprot reference A2QS42.

[0306] Seq ID No. 155: Amino acid sequence of the following: Laccaria bicolor glucosidase, Uniparc reference UPI000164423D, Uniprot reference B0D734.

[0307] Seq ID No. 156: Amino acid sequence of the following: Pedosphaera parvula glucosidase, Uniparc reference UPI00017357F6, Uniprot reference B9XH33.

[0308] Seq ID No. 157: Amino acid sequence of Megamonas hypermegale glucosidase, Uniparc reference UPI00042469F6, Uniprot reference A0A239TGH2.

[0309] Seq ID No. 158: Amino acid sequence of the following: Armillaria gallica glucosidase, Uniparc reference UPI000BC209C3, Uniprot reference A0A2H3E300.

[0310] Seq ID No. 159: Amino acid sequence of Micromonospora sp. glucosidase, Uniparc reference UPI000D2EAE87, Uniprot reference A0A2P8AV03.

[0311] Seq ID No. 160: Amino acid sequence of the following: Klebsiella oxytoca glucosidase, Uniparc reference UPI000DA286EE, Uniprot reference A0A2X5CJC5.

[0312] Seq ID No. 161: Amino acid sequence of the following: Candidatus ozemobacter glucosidase, Uniparc reference UPI000DFAEF6C, Uniprot reference A0A367ZIH8.

[0313] Seq ID No. 162: Amino acid sequence of the following: Coleophoma crateriformis glucosidase, Uniparc reference UPI000E399EE8, Uniprot reference A0A3D8R2C2.

[0314] Seq ID No. 163: Amino acid sequence of the following: Apiotrichum porosum glucosidase, Uniparc reference UPI000FBC01E3, Uniprot reference A0A427XHS2.

[0315] Seq ID No. 164: Amino acid sequence of: Acholeplasma hippikon glucosidase, Uniparc reference UPI00068E4E50, Uniprot reference A0A449BJ27.

[0316] Seq ID No. 165: Amino acid sequence of: Streptomyces spectabilis glucosidase, Uniparc reference UPI001185F074, Uniprot reference A0A516RGT1.

[0317] Seq ID No. 166: Amino acid sequence of the following: Aspergillus niger glucosidase, Uniparc reference UPI0000EFCED2, Uniprot reference A2R8G2.

[0318] Seq ID No. 167: Amino acid sequence of the following: Xanthomonas campestris glucosidase, Uniparc reference UPI00000D8BFA, Uniprot reference B0RYA0.

[0319] Seq ID No. 168: Amino acid sequence of the following: Lactobacillus paracasei glucosidase, Uniparc reference UPI00019C9CD7, Uniprot reference C2FDL2.

[0320] Seq ID No. 169: Amino acid sequence of the following: Bifiguratus adelaidae glucosidase, Uniparc reference UPI000BC490A3, Uniprot reference A0A261XUH4.

[0321] Seq ID No. 170: Amino acid sequence of the following: bacterial glucosidase, Uniparc reference UPI000CC31AE2, Uniprot reference A0A2H5YYA1.

[0322] Seq ID No. 171: Amino acid sequence of the following: Actinomadura parvosata glucosidase, Uniparc reference UPI000D26C9C6, Uniprot reference A0A2P9IY35.

[0323] Seq ID No. 172: Amino acid sequence of the following: Melissococcus plutonius glucosidase, Uniparc reference UPI00024F22B8, Uniprot reference A0A2Z5Y4P3.

[0324] Seq ID No. 173: Amino acid sequence of Enterococcus durans glucosidase, Uniparc reference UPI000E020871, Uniprot reference A0A377KJS3.

[0325] Seq ID No. 174: Amino acid sequence of the following: Malassezia restricta glucosidase, Uniparc reference UPI000DD17A7D, Uniprot reference A0A3G2S2J6.

[0326] Seq ID No. 175: Amino acid sequence of the following: Apiotrichum porosum glucosidase, Uniparc reference UPI000FA2DE87, Uniprot reference A0A427XZQ0.

[0327] Seq ID No. 176: Amino acid sequence of Streptomonospora sp. glucosidase, Uniparc reference UPI0010355193, Uniprot reference A0A4P6PWP5.

[0328] Seq ID No. 177: Amino acid sequence of the following: Lactobacillus gasseri glucosidase, Uniparc reference UPI00119640A1, Uniprot reference A0A558LH47.

[0329] Seq ID No. 178: Amino acid sequence of the following: Aspergillus niger glucosidase, Uniparc reference UPI0000EFD0AA, Uniprot reference A2RAJ1.

[0330] Seq ID No. 179: Amino acid sequence of the following: Neosartorya fumigata glucosidase, Uniparc reference UPI000170BF91, Uniprot reference B0XXG1.

[0331] Seq ID No. 180: Amino acid sequence of the following: Eubacterium eligens glucosidase, Uniparc reference UPI0001A5B234, Uniprot reference C4Z6T5.

[0332] Seq ID No. 181: Amino acid sequence of the following: Bifiguratus adelaidae glucosidase, Uniparc reference UPI000BC64A75, Uniprot reference A0A261XVM8.

[0333] Seq ID No. 182: Amino acid sequence of the following: bacterial glucosidase, Uniparc reference UPI000CAA854E, Uniprot reference A0A2H6EX57.

[0334] Seq ID No. 183: Amino acid sequence of the following: Corynespora cassiicola glucosidase, Uniparc reference UPI000D24EC2C, Uniprot reference A0A2T2N5H9.

[0335] Seq ID No. 184: Amino acid sequence of the following: Pseudomicrostromaglucosiphilum glucosidase, Uniparc reference UPI000D77A0B7, Uniprot reference A0A316UK83.

[0336] Seq ID No. 185: Amino acid sequence of: Staphylococcus saprophyticus glucosidase, Uniparc reference UPI0002DAAFCC, Uniprot reference A0A380HD31.

[0337] Seq ID No. 186: Amino acid sequence of: Malassezia restricta glucosidase, Uniparc reference UPI000F0C6B8E, Uniprot reference A0A3G2S932.

[0338] Seq ID No. 187: Amino acid sequence of: Saitozyma podzolica glucosidase, Uniparc reference UPI000FBA70E2, Uniprot reference A0A427YCL1.

[0339] Seq ID No. 188: Amino acid sequence of the following: Tremella mesenterica glucosidase, Uniparc reference UPI00102822B4, Uniprot reference A0A4Q1BRC6.

[0340] Seq ID No. 189: Amino acid sequence of: Arthrobacter sp. glucosidase, Uniparc reference UPI0000527506, Uniprot reference A0JZ86.

[0341] Seq ID No. 190: Amino acid sequence of the following: Scheffersomyces stipitis glucosidase, Uniparc reference UPI000157388C, Uniprot reference A3LRB0.

[0342] Seq ID No. 191: Amino acid sequence of Leptothrix cholodnii glucosidase, Uniparc reference UPI0001712E02, Uniprot reference B1XZK8.

[0343] Seq ID No. 192: Amino acid sequence of Thauera sp. glucosidase, Uniparc reference UPI000166883C, Uniprot reference C4ZLL7.

[0344] Seq ID No. 193: Amino acid sequence of Kosmotoga olearia glucosidase, Uniparc reference UPI00018494AB, Uniprot reference C5CDW5.

[0345] Seq ID No. 194: Amino acid sequence of Roseburia intestinalis glucosidase, Uniparc reference UPI0001CD671F, Uniprot reference D4L3Y2.

[0346] Seq ID No. 195: Amino acid sequence of Streptococcus equinus glucosidase, Uniparc reference UPI0001E0DC00, Uniprot reference E0PDF8.

[0347] Seq ID No. 196: Amino acid sequence of Streptococcus cristatus glucosidase, Uniparc reference UPI0001F80C4B, Uniprot reference E8JUK5.

[0348] Seq ID No. 197: Amino acid sequence of the following: Cellulosilyticum lentocellum glucosidase, Uniparc reference UPI0001D2DBBC, Uniprot reference F2JLH3.

[0349] Seq ID No. 198: Amino acid sequence of: Streptococcus gallolyticus glucosidase, Uniparc reference UPI0001C48657, Uniprot reference F5WYI5.

[0350] Seq ID No. 199: Amino acid sequence of the following: Ketogulonicigenium vulgare glucosidase, Uniparc reference UPI00021D443A, Uniprot reference F9Y8Z7.

[0351] Seq ID No. 200: Amino acid sequence of the following: Spathaspora passalidarum glucosidase, Uniparc reference UPI000228255D, Uniprot reference G3AGX1.

[0352] Seq ID No. 201: Amino acid sequence of the following: Niastella koreensis glucosidase, Uniparc reference UPI00023F6F5F, Uniprot reference G8T9J3.

[0353] Seq ID No. 202: Amino acid sequence of Cellvibrio sp. glucosidase, Uniparc reference UPI000260108C, Uniprot reference I3IDC0.

[0354] Seq ID No. 203: Amino acid sequence of the following: Flavobacterium sp. glucosidase, Uniparc reference UPI000272D1E0, Uniprot reference J1ACA0.

[0355] Seq ID No. 204: Amino acid sequence of the following: Macrophomina phaseolina glucosidase, Uniparc reference UPI00028E7FE1, Uniprot reference K2S5D3.

[0356] Seq ID No. 205: Amino acid sequence of the following: Kosmotoga olearia glucosidase, Uniparc reference UPI00018483A2, Uniprot reference C5CHI5.

[0357] Seq ID No. 206: Amino acid sequence of the following: Blautia obeum glucosidase, Uniparc reference UPI0001CD5918, Uniprot reference D4LRF6.

[0358] Seq ID No. 207: Amino acid sequence of Bifidobacterium dentium glucosidase, Uniparc reference UPI0001E18CDA, Uniprot reference E0Q541.

[0359] Seq ID No. 208: Amino acid sequence of the following: Anaerolinea thermophila glucosidase, Uniparc reference UPI0001F55F8F, Uniprot reference E8N5R8.

[0360] Seq ID No. 209: Amino acid sequence of the following: Coriobacterium glomerans glucosidase, Uniparc reference UPI0002050DA2, Uniprot reference F2N7E4.

[0361] Seq ID No. 210: Amino acid sequence of the following: Microlunatus phosphovorus glucosidase, Uniparc reference UPI000210C886, Uniprot reference F5XJQ3.

[0362] Seq ID No. 211: Amino acid sequence of Streptomyces sp. glucosidase, Uniparc reference UPI0001C18877, Uniprot reference G0Q1U8.

[0363] Seq ID No. 212: Amino acid sequence of the following: Spathaspora passalidarum glucosidase, Uniparc reference UPI0002282B61, Uniprot reference G3AIV6.

[0364] Seq ID No. 213: Amino acid sequence of the following: Glarea lozoyensis glucosidase, Uniparc reference UPI0002402F4A, Uniprot reference H0ET34.

[0365] Seq ID No. 214: Amino acid sequence of the following: Glaciozyma antarctica glucosidase, Uniparc reference UPI0002633B36, Uniprot reference I3UJK0.

[0366] Seq ID No. 215: Amino acid sequence of: Arthrobacter sp. glucosidase, Uniparc reference UPI00027DFD35, Uniprot reference J7LN00.

[0367] Seq ID No. 216: Amino acid sequence of the following: Fusariumpseudograminearum glucosidase, Uniparc reference UPI00028D698C, Uniprot reference K3VMA9.

[0368] Seq ID No. 217: Amino acid sequence of the following: Hypocrea rufa glucosidase, Uniparc reference UPI000006AA61, Uniprot reference C6GGC9.

[0369] Seq ID No. 218: Amino acid sequence of Ruminococcus torques glucosidase, Uniparc reference UPI0001CDA964, Uniprot reference D4M6W6.

[0370] Seq ID No. 219: Amino acid sequence of Bifidobacterium dentium glucosidase, Uniparc reference UPI0001E17370, Uniprot reference E0Q9Z7.

[0371] Seq ID No. 220: Amino acid sequence of: Bacteroides salanitronis glucosidase, Uniparc reference UPI0001FC71F6, Uniprot reference F0R2D7.

[0372] Seq ID No. 221: Amino acid sequence of the following: Bacteroides coprosuis glucosidase, Uniparc reference UPI00020E6D19, Uniprot reference F3ZQ40.

[0373] Seq ID No. 222: Amino acid sequence of: Marinomonas posidonica glucosidase, Uniparc reference UPI00020D4CC6, Uniprot reference F6CWF6.

[0374] Seq ID No. 223: Amino acid sequence of the following: Chaetomium thermophilum glucosidase, Uniparc reference UPI000227E8ED, Uniprot reference G0SE64.

[0375] Seq ID No. 224: Amino acid sequence of Tetragenococcus halophilus glucosidase, Uniparc reference UPI00022B9A15, Uniprot reference G4L5K1.

[0376] Seq ID No. 225: Amino acid sequence of Paenibacillus sp. glucosidase, Uniparc reference UPI00024F0867, Uniprot reference H6CCZ8.

[0377] Seq ID No. 226: Amino acid sequence of the following: Turneriella parva glucosidase, Uniparc reference UPI000265AA4B, Uniprot reference I4B8U7.

[0378] Seq ID No. 227: Amino acid sequence of: Arthrobacter sp. glucosidase, Uniparc reference UPI00027DFDB2, Uniprot reference J7LQK9.

[0379] Seq ID No. 228: Amino acid sequence of the following: Agaricus bisporus glucosidase, Uniparc reference UPI00029074B1, Uniprot reference K5W7V1.

[0380] Seq ID No. 229: Amino acid sequence of the following: Nectria haematococca glucosidase, Uniparc reference UPI0001B67634, Uniprot reference C7YIP3.

[0381] Seq ID No. 230: Amino acid sequence of Bacteroides xylanisolvens glucosidase, Uniparc reference UPI0001A25287, Uniprot reference D4VSZ0.

[0382] Seq ID No. 231: Amino acid sequence of: Sediminispirochaeta smaragdinae glucosidase, Uniparc reference UPI0001DD9790, Uniprot reference E1R331.

[0383] Seq ID No. 232: Amino acid sequence of: Deinococcus proteolyticus glucosidase, Uniparc reference UPI0001FC42E9, Uniprot reference F0RPV2.

[0384] Seq ID No. 233: Amino acid sequence of: Sphingobacterium sp. glucosidase, Uniparc reference UPI0002033A0A, Uniprot reference F4C226.

[0385] Seq ID No. 234: Amino acid sequence of the following: Sphingobium chlorophenolicum glucosidase, Uniparc reference UPI0001E54133, Uniprot reference F6ET40.

[0386] Seq ID No. 235: Amino acid sequence of the following: Caloramator australicus glucosidase, Uniparc reference UPI00021CACC4, Uniprot reference G0V3V5.

[0387] Seq ID No. 236: Amino acid sequence of: Commensalibacter intestini glucosidase, Uniparc reference UPI000230E3BF, Uniprot reference G6F370.

[0388] Seq ID No. 237: Amino acid sequence of Paenibacillus sp. glucosidase, Uniparc reference UPI00024F07AC, Uniprot reference H6CIT2.

[0389] Seq ID No. 238: Amino acid sequence of: Nitrolancea hollandica glucosidase, Uniparc reference UPI0002638AF3, Uniprot reference I4EIA9.

[0390] Seq ID No. 239: Amino acid sequence of Cryptococcus neoformans glucosidase, Uniparc reference UPI000392C3ED, Uniprot reference J9VVK7.

[0391] Seq ID No. 240: Amino acid sequence of: Acidipropionibacterium acidipropionici glucosidase, Uniparc reference UPI0002988588, Uniprot reference K7S596.

[0392] Seq ID No. 241: Amino acid sequence of Prevotella sp. glucosidase, Uniparc reference UPI0001B93465, Uniprot reference C9PT75.

[0393] Seq ID No. 242: Amino acid sequence of the following: Rhodobacter capsulatus glucosidase, Uniparc reference UPI0001D08095, Uniprot reference D5ALU0.

[0394] Seq ID No. 243: Amino acid sequence of the following: Stigmatella aurantiaca glucosidase, Uniparc reference UPI0001E74370, Uniprot reference E3FJ05.

[0395] Seq ID No. 244: Amino acid sequence of the following: Sphaerochaeta globosa glucosidase, Uniparc reference UPI0002010060, Uniprot reference F0RVK3.

[0396] Seq ID No. 245: Amino acid sequence of the following: Sphaerochaeta coccoides glucosidase, Uniparc reference UPI000207D78C, Uniprot reference F4GH96.

[0397] Seq ID No. 246: Amino acid sequence of: Novosphingobium sp. glucosidase, Uniparc reference UPI00020EFBCB, Uniprot reference F6ICQ5.

[0398] Seq ID No. 247: Amino acid sequence of: Arthrobotrys oligospora glucosidase, Uniparc reference UPI000225331F, Uniprot reference G1XH86.

[0399] Seq ID No. 248: Amino acid sequence of the following: Lactococcus lactis glucosidase, Uniparc reference UPI00000C6907, Uniprot reference G6FFS4.

[0400] Seq ID No. 249: Amino acid sequence of the following: Phaeospirillum molischianum glucosidase, Uniparc reference UPI000255313A, Uniprot reference H8FXH7.

[0401] Seq ID No. 250: Amino acid sequence of the following: Oligococcus marinus glucosidase, Uniparc reference UPI0002609C68, Uniprot reference I4EW72.

[0402] Seq ID No. 251: Amino acid sequence of: Saccharothrix espanaensis glucosidase, Uniparc reference UPI00028AC337, Uniprot reference K0K125.

[0403] Seq ID No. 252: Amino acid sequence of the following: Cronobacter sakazakii glucosidase, Uniparc reference UPI00029BA293, Uniprot reference K8DAK0.

[0404] Seq ID No. 253: Amino acid sequence of the following: Verticillium alfalfae glucosidase, Uniparc reference UPI0001BBDF1E, Uniprot reference C9SVX1.

[0405] Seq ID No. 254: Amino acid sequence of the following: Bacteroides xylanisolvens glucosidase, Uniparc reference UPI0001CCFD36, Uniprot reference D6CY10.

[0406] Seq ID No. 255: Amino acid sequence of the following: Leadbetterella byssophila glucosidase, Uniparc reference UPI0001EBD98A, Uniprot reference E4RUR0.

[0407] Seq ID No. 256: Amino acid sequence of the following: Sphaerochaeta globosa glucosidase, Uniparc reference UPI00020102A5, Uniprot reference F0RYB6.

[0408] Seq ID No. 257: Amino acid sequence of the following: Sphaerochaeta coccoides glucosidase, Uniparc reference UPI000207D6A0, Uniprot reference F4GLH6.

[0409] Seq ID No. 258: Amino acid sequence of the following: Haloplasma contractile glucosidase, Uniparc reference UPI000212252C, Uniprot reference F7Q0Y2.

[0410] Seq ID No. 259: Amino acid sequence of the following: Nitrospirillum amazonense glucosidase, Uniparc reference UPI0002265447, Uniprot reference G1Y4C7.

[0411] Seq ID No. 260: Amino acid sequence of the following: Azospirillum brasilense glucosidase, Uniparc reference UPI00023420B8, Uniprot reference G8AWD9.

[0412] Seq ID No. 261: Amino acid sequence of the following: Phaeospirillum molischianum glucosidase, Uniparc reference UPI000255314F, Uniprot reference H8FXJ8.

[0413] Seq ID No. 262: Amino acid sequence of the following: Oligococcus marinus glucosidase, Uniparc reference UPI000260A2FA, Uniprot reference I4EYD5.

[0414] Seq ID No. 263: Amino acid sequence of the following: Wickerhamomyces ciferrii (Wickerhamomyces ciferrii) glucosidase, Uniparc reference UPI000283EB8D, Uniprot reference K0KVJ2.

[0415] Seq ID No. 264: Amino acid sequence of Gloeocapsa sp. glucosidase, Uniparc reference UPI0002A5D085, Uniprot reference K9XKL8.

[0416] Seq ID No. 265: Amino acid sequence of the following: Sphaerobacter thermophilus glucosidase, Uniparc reference UPI0001A3BCB6, Uniprot reference D1C7U8.

[0417] Seq ID No. 266: Amino acid sequence of the following: Bacteroides xylanisolvens glucosidase, Uniparc reference UPI0001CCEF1F, Uniprot reference D6D4V2.

[0418] Seq ID No. 267: Amino acid sequence of Prevotella buccae glucosidase, Uniparc reference UPI0001F149E8, Uniprot reference E6K4W5.

[0419] Seq ID No. 268: Amino acid sequence of the following: Grosmannia clavigera glucosidase, Uniparc reference UPI0001FF1101, Uniprot reference F0XBR0.

[0420] Seq ID No. 269: Amino acid sequence of the following: Melampsora larici-populina glucosidase, Uniparc reference UPI00020F9774, Uniprot reference F4R4W2.

[0421] Seq ID No. 270: Amino acid sequence of the following: Prevotella multisaccharivorax glucosidase, Uniparc reference UPI0002138E9C, Uniprot reference F8N7G1.

[0422] Seq ID No. 271: Amino acid sequence of Streptomyces zinciresistens glucosidase, Uniparc reference UPI0002255A63, Uniprot reference G2G8K4.

[0423] Seq ID No. 272: Amino acid sequence of the following: Granulicella mallensis glucosidase, Uniparc reference UPI0001D9FC40, Uniprot reference G8NY42.

[0424] Seq ID No. 273: Amino acid sequence of Gibberella zeae glucosidase, Uniparc reference UPI00021F1FEE, Uniprot reference I1RH94.

[0425] Seq ID No. 274: Amino acid sequence of the following: Oligococcus marinus glucosidase, Uniparc reference UPI00026090A2, Uniprot reference I4EYK6.

[0426] Seq ID No. 275: Amino acid sequence of the following: Lactobacillus equicursoris glucosidase, Uniparc reference UPI0002869F55, Uniprot reference K0NRS8.

[0427] Seq ID No. 276: Amino acid sequence of the following: Colletotrichum fructicola glucosidase, Uniparc reference UPI0002A93280, Uniprot reference L2F9W0.

[0428] Seq ID No. 277: Amino acid sequence of the following: Streptosporangium roseum glucosidase, Uniparc reference UPI0001BF8AF6, Uniprot reference D2B261.

[0429] Seq ID No. 278: Amino acid sequence of Listeria grayi glucosidase, Uniparc reference UPI00019F252A, Uniprot reference D7UX19.

[0430] Seq ID No. 279: Amino acid sequence of Enterococcus italicus glucosidase, Uniparc reference UPI0001F11EFF, Uniprot reference E6LF07.

[0431] Seq ID No. 280: Amino acid sequence of the following: Fluviicola taffensis glucosidase, Uniparc reference UPI000203D9EA, Uniprot reference F2IIT6.

[0432] Seq ID No. 281: Amino acid sequence of Shigella flexneri glucosidase, Uniparc reference UPI00020CA641, Uniprot reference F5N4W9.

[0433] Seq ID No. 282: Amino acid sequence of Actinomyces sp. glucosidase, Uniparc reference UPI0002189080, Uniprot reference F9EFW5.

[0434] Seq ID No. 283: Amino acid sequence of the following: Verticillium dahliae glucosidase, Uniparc reference UPI00022EBE5A, Uniprot reference G2X5V6.

[0435] Seq ID No. 284: Amino acid sequence of Actinoplanes sp. glucosidase, Uniparc reference UPI00023ED5A8, Uniprot reference G8S0M0.

[0436] Seq ID No. 285: Amino acid sequence of Gibberella zeae glucosidase, Uniparc reference UPI000023EA29, Uniprot reference I1S320.

[0437] Seq ID No. 286: Amino acid sequence of the following: Auricularia subglabra glucosidase, Uniparc reference UPI00027CE685, Uniprot reference J0WV06.

[0438] Seq ID No. 287: Amino acid sequence of the following: Nitratireductor indicus glucosidase, Uniparc reference UPI00028E86D6, Uniprot reference K2N268.

[0439] Seq ID No. 288: Amino acid sequence of Thermoclostridium stercorarium glucosidase, Uniparc reference UPI0002AD999E, Uniprot reference L7VH66.

[0440] Seq ID No. 289: Amino acid sequence of Geobacillus sp. glucosidase, Uniparc reference UPI0002AF2DE3, Uniprot reference L8A1S2.

[0441] Seq ID No. 290: Amino acid sequence of the following: Uncultured bacterial glucosidase, Uniparc reference UPI000327C3CC, Uniprot reference M9ZC55.

[0442] Seq ID No. 291: Amino acid sequence of Burkholderia ambifaria glucosidase, Uniparc reference UPI000059ACE1, Uniprot reference Q0BAK2.

[0443] Seq ID No. 292: Amino acid sequence of: Aspergillus oryzae glucosidase, Uniparc reference UPI0000676B8D, Uniprot reference Q2UIR4.

[0444] Seq ID No. 293: Amino acid sequence of: Yersinia pseudotuberculosis glucosidase, Uniparc reference UPI00004269B5, Uniprot reference Q66DJ0.

[0445] Seq ID No. 294: Amino acid sequence of Wallemia ichthyophaga glucosidase, Uniparc reference UPI000331CC98, Uniprot reference R9AF64.

[0446] Seq ID No. 295: Amino acid sequence of the following: Glarea lozoyensis glucosidase, Uniparc reference UPI0003522404, Uniprot reference S3DIM6.

[0447] Seq ID No. 296: Amino acid sequence of the following: Moniliophthora roreri glucosidase, Uniparc reference UPI0003BF753A, Uniprot reference V2YU31.

[0448] Seq ID No. 297: Amino acid sequence of Zhouia amylolytica glucosidase, Uniparc reference UPI0003DBCB4F, Uniprot reference W2ULL3.

[0449] Seq ID No. 298: Amino acid sequence of the following: Fusarium oxysporum glucosidase, Uniparc reference UPI0003F362FF, Uniprot reference W9NQQ3.

[0450] Seq ID No. 299: Amino acid sequence of the following: Flavobacterium johnsoniae glucosidase, Uniparc reference UPI00006EA168, Uniprot reference A5FAA5.

[0451] Seq ID No. 300: Amino acid sequence of: Oryza sativa glucosidase, Uniparc reference UPI00002394F5, Uniprot reference Q75193.

[0452] Seq ID No. 301: Amino acid sequence of: Clostridium saccharoperbutylacetonicum glucosidase, Uniparc reference UPI0002B65681, Uniprot reference M1MCD1.

[0453] Seq ID No. 302: Amino acid sequence of: Rhodococcus sp. glucosidase, Uniparc reference UPI0002D21DB1, Uniprot reference N1MBN6.

[0454] Seq ID No. 303: Amino acid sequence of Burkholderia ambifaria glucosidase, Uniparc reference UPI000059C0CD, Uniprot reference Q0BCV8.

[0455] Seq ID No. 304: Amino acid sequence of the following: Xanthomonas campestris glucosidase, Uniparc reference UPI00005CE9E7, Uniprot reference Q3BVH7.

[0456] Seq ID No. 305: Amino acid sequence of: Caulobacter vibrioides glucosidase, Uniparc reference UPI00000C7604, Uniprot reference Q9A6F8.

[0457] Seq ID No. 306: Amino acid sequence of the following: Arcticibacter valbardensis glucosidase, Uniparc reference UPI000338772A, Uniprot reference R9GRA7.

[0458] Seq ID No. 307: Amino acid sequence of Winogradskyella psychrotolerans glucosidase, Uniparc reference UPI00035A4604, Uniprot reference S7VQ28.

[0459] Seq ID No. 308: Amino acid sequence of the following: Methyloglobulus morosus glucosidase, Uniparc reference UPI0003C4ED6A, Uniprot reference V5DXT8.

[0460] Seq ID No. 309: Amino acid sequence of the following: Pestalotiopsis fici glucosidase, Uniparc reference UPI0003E05C04, Uniprot reference W3WV37.

[0461] Seq ID No. 310: Amino acid sequence of the following: Capronia coronata glucosidase, Uniparc reference UPI000434E698, Uniprot reference W9YNR7.

[0462] Seq ID No. 311: Amino acid sequence of: Aspergillus aculeatus glucosidase, Uniparc reference UPI00001268FD, Uniprot reference P48825.

[0463] Seq ID No. 312: Amino acid sequence of the following: Thermotoga neapolitana glucosidase, Uniparc reference UPI0000DD5996, Uniprot reference Q0GC07.

[0464] Seq ID No. 313: Amino acid sequence of: Clostridium saccharoperbutylacetonicum glucosidase, Uniparc reference UPI0002B666E8, Uniprot reference M1MJF4.

[0465] Seq ID No. 314: Amino acid sequence of: Schizosaccharomyces pombe glucosidase, Uniparc reference UPI000006B001, Uniprot reference O74799.

[0466] Seq ID No. 315: Amino acid sequence of: Phaeosphaeria nodorum glucosidase, Uniparc reference UPI000161BD2B, Uniprot reference Q0TXF6.

[0467] Seq ID No. 316: Amino acid sequence of the following: Xylella fastidiosa Dixon glucosidase, Uniparc reference UPI00003806C8, Uniprot reference Q3RGJ3.

[0468] Seq ID No. 317: Amino acid sequence of: Schizosaccharomyces pombe glucosidase, Uniparc reference UPI000006A330, Uniprot reference Q9P6J6.

[0469] Seq ID No. 318: Amino acid sequence of the following: Arcticibacter valbardensis glucosidase, Uniparc reference UPI000337E887, Uniprot reference R9GWD6.

[0470] Seq ID No. 319: Amino acid sequence of the following: Colletotrichum gloeosporioides glucosidase, Uniparc reference UPI0003885717, Uniprot reference T0KJ17.

[0471] Seq ID No. 320: Amino acid sequence of the following: Uncultured bacterial glucosidase, Uniparc reference UPI0003C9E340, Uniprot reference V5R1E8.

[0472] Seq ID No. 321: Amino acid sequence of the following: Xanthomonas arboricola glucosidase, Uniparc reference UPI0003E06A61, Uniprot reference W4S7I5.

[0473] Seq ID No. 322: Amino acid sequence of the following: Fusarium oxysporum glucosidase, Uniparc reference UPI00021EC697, Uniprot reference X0A8X8.

[0474] Seq ID No. 323: Amino acid sequence of: Paenibacillus polymyxa glucosidase, Uniparc reference UPI00001108D0, Uniprot reference P22073.

[0475] Seq ID No. 324: Amino acid sequence of the following: Kluyveromyces marxianus glucosidase, Uniparc reference UPI0001BE5ADA, Uniprot reference D1GCC6.

[0476] Seq ID No. 325: Amino acid sequence of the following: Ilumatobacter coccineus glucosidase, Uniparc reference UPI0002C04A25, Uniprot reference M5A594.

[0477] Seq ID No. 326: Amino acid sequence of: Agrobacterium sp. glucosidase, Uniparc reference UPI0000126912, Uniprot reference P12614.

[0478] Seq ID No. 327: Amino acid sequence of the following: Cytophaga hutchinsonii glucosidase, Uniparc reference UPI000038ECF7, Uniprot reference Q11P53.

[0479] Seq ID No. 328: Amino acid sequence of Thermobifida fusca glucosidase, Uniparc reference UPI00003C5CA4, Uniprot reference Q47PF5.

[0480] Seq ID No. 329: Amino acid sequence of the following: Botryotinia fuckeliana glucosidase, Uniparc reference UPI0000069E5E, Uniprot reference Q9UVJ6.

[0481] Seq ID No. 330: Amino acid sequence of: Agarivorans albus glucosidase, Uniparc reference UPI00033994D2, Uniprot reference R9PTK2.

[0482] Seq ID No. 331: Amino acid sequence of Enterococcus sp. glucosidase, Uniparc reference UPI00038B7639, Uniprot reference TOUDU2.

[0483] Seq ID No. 332: Amino acid sequence of: Salinispira pacifica glucosidase, Uniparc reference UPI0003D8A7DC, Uniprot reference V5WKT4.

[0484] Seq ID No. 333: Amino acid sequence of Bacteroides xylanisolvens glucosidase, Uniparc reference UPI0003ECF15E, Uniprot reference W6P696.

[0485] Seq ID No. 334: Amino acid sequence of Fusarium oxysporum glucosidase, Uniparc reference UPI000430029A, Uniprot reference X0AB46.

[0486] Seq ID No. 335: Amino acid sequence of Thermotoga maritima glucosidase, Uniparc reference UPI0000126906, Uniprot reference Q08638B.

[0487] Seq ID No. 336: Amino acid sequence of the following: Neotermes koshunensis glucosidase, Uniparc reference number UPI0000083EA8, Uniprot reference number Q8T0W7.

[0488] Seq ID No. 337: Amino acid sequence of the following: Thanatephorus cucumeris glucosidase, Uniparc reference UPI0002BF34B3, Uniprot reference M5CHG9.

[0489] Seq ID No. 338: Amino acid sequence of: Hungateiclostridium thermocellum glucosidase, Uniparc reference UPI000053581D, Uniprot reference P14002.

[0490] Seq ID No. 339: Amino acid sequence of the following: Koribacter versatilis glucosidase, Uniparc reference UPI0000D76A70, Uniprot reference Q1IJ89.

[0491] Seq ID No. 340: Amino acid sequence of the following: Neosartorya fumigata glucosidase, Uniparc reference UPI00005203D8, Uniprot reference Q4WLX5.

[0492] Seq ID No. 341: Amino acid sequence of the following: Amycolatopsis vancoresmycina glucosidase, Uniparc reference UPI00032DB8C1, Uniprot reference R1FKF0.

[0493] Seq ID No. 342: Amino acid sequence of Gibberella fujikuroi glucosidase, Uniparc reference UPI0003519C76, Uniprot reference S0EKU1.

[0494] Seq ID No. 343: Amino acid sequence of the following: Bifidobacterium longum glucosidase, Uniparc reference UPI000390F839, Uniprot reference T2I2H5.

[0495] Seq ID No. 344: Amino acid sequence of: Uncultured bacterial glucosidase, Uniparc reference UPI0003DFF0DA, Uniprot reference W0FLD1.

[0496] Seq ID No. 345: Amino acid sequence of the following: Bacteroides xylanisolvens glucosidase, Uniparc reference UPI0002D3D994, Uniprot reference W6P9J9.

[0497] Seq ID No. 346: Amino acid sequence of: Fusarium oxysporum glucosidase, Uniparc reference UPI00042F8016, Uniprot reference X0HA35.

[0498] Seq ID No. 347: Amino acid sequence of the following: Sorghum bicolor glucosidase, Uniparc reference UPI00000A7F40, Uniprot reference Q41290.

[0499] Seq ID No. 348: Amino acid sequence of Clostridium cellulovorans glucosidase, Uniparc reference UPI000050B701, Uniprot reference Q53EH2.

[0500] Seq ID No. 349: Amino acid sequence of the following: Dacryopinax primogenitus glucosidase, Uniparc reference UPI0002C29EAA, Uniprot reference M5G1U5.

[0501] Seq ID No. 350: Amino acid sequence of the following: Rhizobium radiobacter glucosidase, Uniparc reference UPI0000126913, Uniprot reference P27034.

[0502] Seq ID No. 351: Amino acid sequence of: Phanerochaetechrysosporium glucosidase, Uniparc reference UPI00006E0CC7, Uniprot reference Q25BW5A.

[0503] Seq ID No. 352: Amino acid sequence of Enterobacter agglomerans glucosidase, Uniparc reference UPI0000126905, Uniprot reference Q59437.

[0504] Seq ID No. 353: Amino acid sequence of: Candidatus microthrix glucosidase, Uniparc reference UPI00033056C4, Uniprot reference R4YWU4.

[0505] Seq ID No. 354: Amino acid sequence of Chthonomonas calidirosea glucosidase, Uniparc reference UPI0003427F29, Uniprot reference S0ET37.

[0506] Seq ID No. 355: The following amino acid sequence: Acholeplasma brassicae glucosidase, Uniparc reference UPI0003B04208, Uniprot reference U4KMR7. Seq ID No. 356: The following amino acid sequence: Chania multitudinisentens glucosidase, Uniparc reference UPI0003E13BD3, Uniprot reference W0LJ28.

[0507] Seq ID No. 357: Amino acid sequence of Gibberella moniliformis glucosidase, Uniparc reference UPI0003ECCF80, Uniprot reference W7MW98.

[0508] Seq ID No. 358: Amino acid sequence of the following: Fusarium oxysporum glucosidase, Uniparc reference UPI0004305856, Uniprot reference X0I2U8.

[0509] Seq ID No. 359: Amino acid sequence of: Phanerochaetechrysosporium glucosidase, Uniparc reference UPI00006E0CC7, Uniprot reference Q25BW5B.

[0510] Seq ID No. 360: Amino acid sequence of: Secale cereale glucosidase, Uniparc reference UPI00000A7EFC, Uniprot reference Q9FYS3.

[0511] Seq ID No. 361: Amino acid sequence of the following: Anoxybacillus gonensis glucosidase, Uniparc reference UPI0002C0273E, Uniprot reference M5QUM2.

[0512] Seq ID No. 362: Amino acid sequence of Thermotoga maritima glucosidase, Uniparc reference UPI0000126906, Uniprot reference Q08638A.

[0513] Seq ID No. 363: Amino acid sequence of the following: Rhodospirillum rubrum glucosidase, Uniparc reference UPI00003C2ACC, Uniprot reference Q2RP51.

[0514] Seq ID No. 364: Amino acid sequence of: Thermotoga neapolitana glucosidase, Uniparc reference UPI00000B9013, Uniprot reference Q60038.

[0515] Seq ID No. 365: Amino acid sequence of the following: Candidatus microthrix glucosidase, Uniparc reference UPI00032F466E, Uniprot reference R4Z6M6.

[0516] Seq ID No. 366: Amino acid sequence of: Ruminiclostridium cellobioparum glucosidase, Uniparc reference UPI00032874EC, Uniprot reference SOFPI8.

[0517] Seq ID No. 367: Amino acid sequence of: Acholeplasma brassicae glucosidase, Uniparc reference UPI0003B0490C, Uniprot reference U4KMV4.

[0518] Seq ID No. 368: Amino acid sequence of the following: Klebsiella pneumoniae glucosidase, Uniparc reference UPI0003DB6EFD, Uniprot reference W1BBP5.

[0519] Seq ID No. 369: Amino acid sequence of Enterobacter sp. glucosidase, Uniparc reference UPI0003ED0A8E, Uniprot reference W7P6Y1.

[0520] Seq ID No. 370: Amino acid sequence of the following: Rhizoctonia solani glucosidase, Uniparc reference UPI00045BB507, Uniprot reference X8JI88.

[0521] Seq ID No. 371: Amino acid sequence of Homo sapiens glucosidase, Uniparc reference UPI0000072C73, Uniprot reference Q9H227.

[0522] Seq ID No. 372: Amino acid sequence of: Uncultured bacterial glucosidase, Uniparc reference UPI0000DD5304, Uniprot reference Q0GMU3.

[0523] Seq ID No. 373: Amino acid sequence of Thermoanaerobacter thermohydrosulfuricus glucosidase, Uniparc reference UPI0002CA9E4F, Uniprot reference M8CQD9.

[0524] Seq ID No. 374: Amino acid sequence of the following: Stigmatella aurantiaca glucosidase, Uniparc reference UPI0000E297D8, Uniprot reference Q091M8.

[0525] Seq ID No. 375: Amino acid sequence of the following: Burkholderia thailandensis glucosidase, Uniparc reference UPI00006676B1, Uniprot reference Q2T7C9.

[0526] Seq ID No. 376: Amino acid sequence of: Yersinia pseudotuberculosis glucosidase, Uniparc reference UPI0000426E8F, Uniprot reference Q665S2.

[0527] Seq ID No. 377: Amino acid sequence of the following: Togninia minima glucosidase, Uniparc reference UPI00032BF71F, Uniprot reference R8BQM8.

[0528] Seq ID No. 378: Amino acid sequence of: Ophiostoma piceae glucosidase, Uniparc reference UPI00035213C2, Uniprot reference S3BVJ8.

[0529] Seq ID No. 379: Amino acid sequence of the following: Pyronema omphalodes glucosidase, Uniparc reference UPI0003B117B7, Uniprot reference U4LIL3.

[0530] Seq ID No. 380: Amino acid sequence of the following: Ogataea parapolymorpha glucosidase, Uniparc reference UPI0001F76D1F, Uniprot reference W1QIW0.

[0531] Seq Id No. 381: Amino acid sequence of the following: Hymenobacter swuensis glucosidase, Uniparc reference UPI0003F2059F, Uniprot reference W8F6U0.

[0532] Seq ID No. 382: Amino acid sequence of the following: Flavobacterium johnsoniae glucosidase, Uniparc reference UPI00006E8E1B, Uniprot reference A5FEF5.

[0533] Seq ID No. 383: Amino acid sequence of: Paenibacillus polymyxa glucosidase, Uniparc reference UPI000012690B, Uniprot reference P22505.

[0534] Seq ID No. 384: Amino acid sequence of: Rice (Oryza sativa) glucosidase, Uniparc reference UPI000009D014, Uniprot reference Q8L7J2.

[0535] Seq ID No. 385: Amino acid sequence of: Rice (Oryza sativa) glucosidase, Uniparc reference UPI0000E580F2, Uniprot reference B8AVF0.

[0536] Seq ID No. 386: Amino acid sequence of the following: Nannochloris glucosidase, Uniparc reference UPI000EA7F16F, Uniprot reference A0A452CSM4.

[0537] Seq ID No. 387: Amino acid sequence of Halothermothrix orenii glucosidase, Uniparc reference UPI00006AE508, Uniprot reference B8CYA8.

[0538] Seq ID No. 388: Amino acid sequence of Neurospora crassa glucosidase, Uniparc reference UPI000018B2B4, Uniprot reference Q7RWP2.

[0539] Seq ID No. 389: Amino acid sequence of the following: Micrococcus antarcticus glucosidase, Uniparc reference UPI000192BB5F, Uniprot reference B9V8P5.

[0540] Seq ID No. 390: Amino acid sequence of Exiguobacterium antarcticum glucosidase, Uniparc reference UPI000285E79E, Uniprot reference K0A8J9.

[0541] Seq ID No. 391: Amino acid sequence of Thermus thermophilus glucosidase, Uniparc reference UPI00000BEB61, Uniprot reference Q9RA61.

[0542] Seq ID No. 392: Amino acid sequence of Trichoderma harzianum glucosidase, Uniparc reference UPI00078BF747, Uniprot reference A0A2T4AR08.

[0543] Seq ID No. 393: Amino acid sequence of: Hypocrea jecorina glucosidase, Uniparc reference UPI000006AA61, Uniprot reference Q12715.

[0544] Seq ID No. 394: Amino acid sequence of Streptomyces sp. glucosidase, Uniparc reference UPI00000B411B, Uniprot reference Q59976.

[0545] Seq ID No. 395: Amino acid sequence of: Streptococcus pyogenes glucosidase, Uniparc reference UPI00000C7E56, Uniprot reference Q99YP9.

[0546] Seq ID No. 396: Amino acid sequence of the following: Trifolium repens glucosidase, Uniparc reference UPI000012691B, Uniprot reference P26205.

[0547] Seq ID No. 397: Amino acid sequence of the following: Talaromyces emersonii glucosidase, Uniparc reference UPI000006C8FF, Uniprot reference Q8TGI8.

[0548] Seq ID No. 398: Amino acid sequence of: Hungateiclostridium thermocellum glucosidase, Uniparc reference UPI0000126903, Uniprot reference P26208.

[0549] Seq ID No. 399: Amino acid sequence of the following: Lactobacillus plantarum glucosidase, Uniparc reference UPI000219FE3E, Uniprot reference F9ULH8.

[0550] Seq ID No. 400: Amino acid sequence of: Agrobacterium tumefaciens glucosidase, Uniparc reference UPI0003F2033A, Uniprot reference A0A2I4PGZ0.

[0551] Seq ID No. 401: Codon-optimized DNA encoding 6xHis Cyberlindnera fabianii glucosidase, Uniparc reference UPI00049B1A8C, Uniprot reference A0A061B3J2.

[0552] Seq ID No. 402: Codon-optimized DNA encoding 6xHis Flavobacterium gilvum glucosidase, Uniparc reference UPI0004E3EF7B, Uniprot reference A0A085EII0.

[0553] Seq ID No. 403: Codon-optimized DNA encoding 6xHis Algibacterlectus glucosidase, Uniparc reference UPI00050EE490, Uniprot reference A0A090X649.

[0554] Seq ID No. 404: Codon-optimized DNA encoding 6xHis Microbacterium azadirachtae glucosidase, Uniparc reference UPI0005ECB51E, Uniprot reference A0A0F0LB94.

[0555] Seq ID No. 405: Codon-optimized DNA encoding 6xHis Actinobacteriabacterium glucosidase, Uniparc reference UPI0006588DAD, Uniprot reference A0A0JOUT37.

[0556] Seq Id No. 406: Codon-optimized DNA encoding 6xHis Chloroflexibacterium glucosidase, Uniparc reference UPI0007968552, Uniprot reference A0A136KWB3.

[0557] Seq ID No. 407: Codon-optimized DNA encoding 6xHis Komagataeibacterrhaeticus glucosidase, Uniparc reference UPI0002080410, Uniprot reference A0A181C809.

[0558] Seq ID No. 408: Codon-optimized DNA encoding 6xHis Bacteroides sp. glucosidase, Uniparc reference UPI0008211BFC, Uniprot reference A0A1C5WEL8.

[0559] Seq ID No. 409: Codon-optimized DNA encoding 6xHis Streptomyces rubrolavendulae glucosidase, Uniparc reference UPI00085A2BD0, Uniprot reference A0A1D8FZW3.

[0560] Seq ID No. 410: Codon-optimized DNA encoding 6xHis Streptomyces rubrolavendulae glucosidase, Uniparc reference UPI00098C60F6, Uniprot reference A0A1S8KYM5.

[0561] Seq ID No. 411: Codon-optimized DNA encoding 6xHis uncultured bacterial glucosidase, Uniparc reference UPI0009CE0D4C, Uniprot reference A0A1V5M6V6.

[0562] Seq ID No. 412: Codon-optimized DNA encoding 6xHis Firmicutes bacteria glucosidase, Uniparc reference UPI0009D4127D, Uniprot reference A0A1V6AN95.

[0563] Seq ID No. 413: Codon-optimized DNA encoding 6xHis Anthracocystis flocculosa glucosidase, Uniparc reference UPI00045601AB, Uniprot reference A0A061H1Z3.

[0564] Seq ID No. 414: Codon-optimized DNA encoding 6xHis Bifidobacterium boum glucosidase, Uniparc reference UPI0004FF77C7, Uniprot reference A0A086ZKU2.

[0565] Seq ID No. 415: Codon-optimized DNA encoding 6xHis Jejuia pallidilutea glucosidase, Uniparc reference UPI00051EDBDE, Uniprot reference A0A098LTR2.

[0566] Seq ID No. 416: Codon-optimized DNA encoding 6xHis Ceratocystis fimbriata glucosidase, Uniparc reference UPI00062105AB, Uniprot reference A0A0F8B2B0.

[0567] Seq ID No. 417: Codon-optimized DNA encoding 6xHis Actinobacteriabacterium glucosidase, Uniparc reference UPI0006583AB1, Uniprot reference A0A0J0UVW7.

[0568] Seq ID No. 418: Codon-optimized DNA encoding 6xHis Rhodococcus sp. glucosidase, Uniparc reference UPI0007AABFAD, Uniprot reference A0A143QAX3.

[0569] Seq ID No. 419: Codon-optimized DNA encoding 6xHis Valsa mali glucosidase, Uniparc reference UPI0007F2D02D, Uniprot reference A0A194VF47.

[0570] Seq ID No. 420: Codon-optimized DNA encoding 6xHis uncultured Bacteroides sp. glucosidase, Uniparc reference UPI000821004D, Uniprot reference A0A1C5WSI4.

[0571] Seq ID No. 421: Codon-optimized DNA encoding 6xHis Eisenbergiella tayi glucosidase, Uniparc reference UPI00084089B2, Uniprot reference A0A1E3ALT2.

[0572] SeqID No. 422: Codon-optimized DNA encoding 6xHis Streptomyces sp. glucosidase, Uniparc reference UPI000978E914, Uniprot reference A0A1V2MYI4.

[0573] Seq ID No. 423: Codon-optimized DNA encoding 6xHis Firmicutes bacteria glucosidase, Uniparc reference UPI0009CBF21C, Uniprot reference A0A1V5MH90.

[0574] Seq ID No. 424: Codon-optimized DNA encoding 6xHis Tenericutesbacterium glucosidase, Uniparc reference UPI0009D5B1F0, Uniprot reference A0A1V6BAK3.

[0575] Seq ID No. 425: Codon-optimized DNA encoding 6xHis Gluconobacteroxydans glucosidase, Uniparc reference UPI0004A87350, Uniprot reference A0A067Z479.

[0576] Seq ID No. 426: Codon-optimized DNA encoding 6xHis Bifidobacterium catenulatum glucosidase, Uniparc reference UPI00050787A2, Uniprot reference A0A087B8Q8.

[0577] Seq ID No. 427: Codon-optimized DNA encoding 6xHis Bionectria ochroleuca glucosidase, Uniparc reference UPI0005965863, Uniprot reference A0A0B7K538.

[0578] Seq ID No. 428: Codon-optimized DNA encoding 6xHis Parcubacteria sp. glucosidase, Uniparc reference UPI0006377CA4, Uniprot reference A0A0G0GD78.

[0579] Seq ID No. 429: Codon-optimized DNA encoding 6xHis Microbacterium ketosireducens glucosidase, Uniparc reference UPI0006228575, Uniprot reference A0A0M2H276.

[0580] Seq ID No. 430: Codon-optimized DNA encoding 6xHis Roseburia faecis glucosidase, Uniparc reference UPI0006C454B4, Uniprot reference A0A173R3W4.

[0581] Seq ID No. 431: Codon-optimized DNA encoding 6xHis Kwoniella dejecticola glucosidase, Uniparc reference UPI0007F1D695, Uniprot reference A0A1A6A050.

[0582] Seq ID No. 432: Codon-optimized DNA encoding 6xHis uncultured Clostridium sp. glucosidase, Uniparc reference UPI0008232A70, Uniprot reference A0A1C6C862.

[0583] Seq ID No. 433: Codon-optimized DNA encoding 6xHis Cyberlindnera jadinii glucosidase, Uniparc reference UPI000866CB30, Uniprot reference A0A1E4S2F8.

[0584] Seq ID No. 434: Codon-optimized DNA encoding 6xHis Bacteroidetesbacterium glucosidase, Uniparc reference UPI0009D3483D, Uniprot reference A0A1V5G4W6.

[0585] Seq ID No. 435: Codon-optimized DNA encoding 6xHis Verrucomicrobiabacterium glucosidase, Uniparc reference UPI0009CC9AEA, Uniprot reference A0A1V5Q4R4.

[0586] Seq ID No. 436: Codon-optimized DNA encoding 6xHis Bacteroidetesbacterium glucosidase, Uniparc reference UPI0009C6DE73, Uniprot reference A0A1V6BV25.

[0587] Seq ID No. 437: Codon-optimized DNA encoding 6xHis Lichtheimia ramosa glucosidase, Uniparc reference UPI0004E051A9, Uniprot reference A0A077WUK7.

[0588] Seq ID No. 438: Codon-optimized DNA encoding 6xHis Bifidobacterium mongoliense glucosidase, Uniparc reference UPI0005060F52, Uniprot reference A0A087BWT7.

[0589] Seq ID No. 439: Codon-optimized DNA encoding 6xHis Vibrio ishigakensis glucosidase, Uniparc reference UPI000591CED9, Uniprot reference A0A0B8NZY1.

[0590] Seq ID No. 440: Codon-optimized DNA encoding 6xHis Phaeomoniella chlamydospora glucosidase, Uniparc reference UPI00063B706F, Uniprot reference A0A0G2HEV5.

[0591] Seq ID No. 441: Codon-optimized DNA encoding 6xHis Ardenticatena maritima glucosidase, Uniparc reference UPI0006C04F59, Uniprot reference A0A0M8K5H7.

[0592] Seq ID No. 442: Codon-optimized DNA encoding 6xHis Coprococcus comens glucosidase, Uniparc reference UPI000197E031, Uniprot reference A0A173WPC4.

[0593] Seq ID No. 443: Codon-optimized DNA encoding 6xHis Nocardioides dokdorensis glucosidase, Uniparc reference UPI0007DDBAB3, Uniprot reference A0A1A9GNJ0.

[0594] Seq ID No. 444: Codon-optimized DNA encoding 6xHis uncultured Clostridium sp. glucosidase, Uniparc reference UPI000822F7EB, Uniprot reference A0A1C6EBC1.

[0595] Seq ID No. 445: Codon-optimized DNA encoding 6xHis Acetobacterium wieringae glucosidase, Uniparc reference UPI000878EB40, Uniprot reference A0A1F2PFB4.

[0596] Seq ID No. 446: Codon-optimized DNA encoding 6xHis Tenericutesbacterium glucosidase, Uniparc reference UPI0009D0A2C1, Uniprot reference A0A1V5HNJ3.

[0597] Seq ID No. 447: Codon-optimized DNA encoding 6xHis Tenericutesbacterium glucosidase, Uniparc reference UPI0009CB8A73, Uniprot reference A0A1V5UFI1.

[0598] Seq ID No. 448: Codon-optimized DNA encoding 6xHis uncultured bacterial glucosidase, Uniparc reference UPI0009CC90AF, Uniprot reference A0A1V6CDT2.

[0599] Seq ID No. 449: Codon-optimized DNA encoding 6xHis Parabacteroides distasonis glucosidase, Uniparc reference UPI0004D8E473, Uniprot reference A0A078SYD0.

[0600] Seq ID No. 450: Codon-optimized DNA encoding 6xHis Bifidobacterium psychraerophilum glucosidase, Uniparc reference UPI0005006C6A, Uniprot reference A0A087CJB0.

[0601] Seq ID No. 451: Codon-optimized DNA encoding 6xHis Hebeloma cylindrosporum glucosidase, Uniparc reference UPI00059A3BA8, Uniprot reference A0A0C2YJF2.

[0602] Seq ID No. 452: Codon-optimized DNA encoding 6xHis Brenneria goodwinii glucosidase, Uniparc reference UPI0006579FEA, Uniprot reference A0A0G4JRR8.

[0603] Seq ID No. 453: Codon-optimized DNA encoding 6xHis Aspergillus calidoustus glucosidase, Uniparc reference UPI00073C9E60, Uniprot reference A0A0U5GN20.

[0604] Seq ID No. 454: Codon-optimized DNA encoding 6xHis Bacteroides finegoldii glucosidase, Uniparc reference UPI0006C6E0C3, Uniprot reference A0A174BEZ2.

[0605] Seq ID No. 455: Codon-optimized DNA encoding 6xHis Altererythrobacter dongtanensis glucosidase, Uniparc reference UPI0008153D6D, Uniprot reference A0A1B2A943.

[0606] Seq ID No. 456: Codon-optimized DNA encoding 6xHis uncultured Anaerotruncus sp. glucosidase, Uniparc reference UPI0008206E7B, Uniprot reference A0A1C6FWD9.

[0607] Seq ID No. 457: Codon-optimized DNA encoding 6xHis Candidatus firestone glucosidase, Uniparc reference UPI0008AC0E17, Uniprot reference A0A1F5UJR6.

[0608] Seq ID No. 458: Codon-optimized DNA encoding 6xHis Tenericutes bacterium glucosidase, Uniparc reference UPI0009C6C8E4, Uniprot reference A0A1V5HS22.

[0609] Seq ID No. 459: Codon-optimized DNA encoding 6xHis glucosidase from Lentisphaerae bacterium, Uniparc reference UPI0009CEE1AB, Uniprot reference A0A1V5VBL2.

[0610] Seq ID No. 460: Codon-optimized DNA encoding 6xHis Planctomycetes bacterium glucosidase, Uniparc reference UPI0009CAAB0A, Uniprot reference A0A1V6FZ47.

[0611] Seq ID No. 461: Codon-optimized DNA encoding 6xHis Pseudallscheria apiosperma glucosidase, Uniparc reference UPI0004DD62AC, Uniprot reference A0A084G332.

[0612] Seq ID No. 462: Codon-optimized DNA encoding 6xHis Nonlabens sediminis glucosidase, Uniparc reference UPI000507F00A, Uniprot reference A0A090Q4N8.

[0613] Seq ID No. 463: Codon-optimized DNA encoding 6xHis Gynuella sunshinyii glucosidase, Uniparc reference UPI0005CC42CA, Uniprot reference A0A0C5VDU3.

[0614] Seq ID No. 464: Codon-optimized DNA encoding 6xHis Verticillium longisporum glucosidase, Uniparc reference UPI00063E4005, Uniprot reference A0A0G4N9Q7.

[0615] Seq ID No. 465: Codon-optimized DNA encoding 6xHis Cellulomonas sp. glucosidase, Uniparc reference UPI00073C6CD3, Uniprot reference A0A0V8TAB5.

[0616] Seq ID No. 466: Codon-optimized DNA encoding 6xHis Hungatella hathewayi glucosidase, Uniparc reference UPI0006C024BB, Uniprot reference A0A174FBX7.

[0617] Seq ID No. 467: Codon-optimized DNA encoding 6xHis Mesorhizobium sp. glucosidase, Uniparc reference UPI000688E74D, Uniprot reference A0A1C2DG64.

[0618] Seq ID No. 468: Codon-optimized DNA encoding 6xHis Clostridium sp. glucosidase, Uniparc reference UPI000822FAB7, Uniprot reference A0A1C6GRT5.

[0619] Seq ID No. 469: Codon-optimized DNA encoding 6xHis Chlamydialesbacterium glucosidase, Uniparc reference UPI0009284E74, Uniprot reference A0A1M3CSY6.

[0620] Seq ID No. 470: Codon-optimized DNA encoding 6xHis Spirochaetesbacterium glucosidase, Uniparc reference UPI0009CB461D, Uniprot reference A0A1V5HUX3.

[0621] Seq ID No. 471: Codon-optimized DNA encoding 6xHis bacterial glucosidase, Uniparc reference UPI0009C92D6A, Uniprot reference A0A1V5VHB9.

[0622] Seq ID No. 472: Codon-optimized DNA encoding 6xHis Thermotogaebacterium glucosidase, Uniparc reference UPI0009D12B86, Uniprot reference A0A1V6H2W5.

[0623] Seq ID No. 473: Codon-optimized DNA encoding 6xHis Pseudomonas apiosperma glucosidase, Uniparc reference UPI0004DCBED3, Uniprot reference A0A084GGE2.

[0624] Seq ID No. 474: Codon-optimized DNA encoding 6xHis Algibacterlectus glucosidase, Uniparc reference UPI00050E0BE3, Uniprot reference A0A090VF17.

[0625] Seq ID No. 475: Codon-optimized DNA encoding 6xHis Paxillus involutus glucosidase, Uniparc reference UPI0005B075C8, Uniprot reference A0A0C9TWP5.

[0626] Seq ID No. 476: Codon-optimized DNA encoding 6xHis Verticillium longisporum glucosidase, Uniparc reference UPI00063DF006, Uniprot reference A0A0G4NA55.

[0627] Seq ID No. 477: Codon-optimized DNA encoding 6xHis Mucilaginibacter gotjawali glucosidase, Uniparc reference UPI00076F8EA4, Uniprot reference A0A110B1H1. Seq ID No. 478: Codon-optimized DNA encoding 6xHis Bacteroides uniformis glucosidase, Uniparc reference UPI0006C0FFEF, Uniprot reference A0A174IWW4.

[0628] Seq ID No. 479: Codon-optimized DNA encoding 6xHis Coprococcus sp. glucosidase, Uniparc reference UPI0008222B77, Uniprot reference A0A1C5W0N6.

[0629] Seq ID No. 480: Codon-optimized DNA encoding 6xHis Blautia sp. glucosidase, Uniparc reference UPI0006C36823, Uniprot reference A0A1C6K2X5.

[0630] Seq ID No. 481: Codon-optimized DNA encoding 6xHis Cellulomonas sp. glucosidase, Uniparc reference UPI00092B3CD4, Uniprot reference A0A1M3ELH4.

[0631] Seq ID No. 482: Codon-optimized DNA encoding 6xHis bacterial glucosidase, Uniparc reference UPI0009C85428, Uniprot reference A0A1V5J984.

[0632] Seq ID No. 483: Codon-optimized DNA encoding 6xHis Spirochaetesbacterium glucosidase, Uniparc reference UPI0009CA1D5C, Uniprot reference A0A1V5WHQ7.

[0633] Seq ID No. 484: Codon-optimized DNA encoding 6xHis Tenericutesbacterium glucosidase, Uniparc reference UPI0009C7041F, Uniprot reference A0A1V6IJ57.

[0634] Seq ID No. 485: Codon-optimized DNA encoding 6xHis Flavobacterium gilvum glucosidase, Uniparc reference UPI0004E2A41F, Uniprot reference A0A085EG29.

[0635] Seq ID No. 486: Codon-optimized DNA encoding 6xHis Algibacterlectus glucosidase, Uniparc reference UPI0005102470, Uniprot reference A0A090WWZ1.

[0636] Seq ID No. 487: Codon-optimized DNA encoding 6xHis Hydnomerulius pinastri glucosidase, Uniparc reference UPI0005B0F0AE, Uniprot reference A0A0C9WDY0. Seq ID No. 488: Codon-optimized DNA encoding 6xHis Nocardia farcinica glucosidase, Uniparc reference UPI00065C2666, Uniprot reference A0A0H5NWN2.

[0637] Seq ID No. 489: Codon-optimized DNA encoding 6xHis Bacteroides cellulosilyticus glucosidase, Uniparc reference UPI000760375B, Uniprot reference A0A125MG18.

[0638] Seq ID No. 490: Codon-optimized DNA encoding 6xHis Fonsecaea erecta glucosidase, Uniparc reference UPI0007DF4250, Uniprot reference A0A178ZBN2.

[0639] Seq ID No. 491: Codon-optimized DNA encoding 6xHis Bacteroides sp. glucosidase, Uniparc reference UPI0008209852, Uniprot reference A0A1C5W9N9.

[0640] Seq ID No. 492: Codon-optimized DNA encoding 6xHis Tannerella forsythia glucosidase, Uniparc reference UPI00086C34C1, Uniprot reference A0A1D3UGH8.

[0641] Seq ID No. 493: Codon-optimized DNA encoding 6xHis Microbacterium esteraromaticum glucosidase, Uniparc reference UPI00097E83BB, Uniprot reference A0A1R4KI92.

[0642] Seq ID No. 494: Codon-optimized DNA encoding 6xHis bacterial glucosidase, Uniparc reference UPI0009D255E5, Uniprot reference A0A1V5LJK9.

[0643] Seq ID No. 495: Codon-optimized DNA encoding 6xHis Candidatus hydrogenedentes glucosidase, Uniparc reference UPI0009C5A3CF, Uniprot reference A0A1V522L2.

[0644] Seq ID No. 496: Codon-optimized DNA encoding 6xHis Bacteroidetesbacterium glucosidase, Uniparc reference UPI0009C55799, Uniprot reference A0A1V6J4J8.

[0645] Seq ID No. 497: Codon-optimized DNA encoding 6xHis Penicillium solitum glucosidase, Uniparc reference UPI0009D4067F, Uniprot reference A0A1V6RQ41.

[0646] Seq ID No. 498: Codon-optimized DNA encoding 6xHis Weissella soli glucosidase, Uniparc reference UPI0008737AA2, Uniprot reference A0A288Q8I2.

[0647] Seq ID No. 499: Codon-optimized DNA encoding 6xHis Acetatifactor muris glucosidase, Uniparc reference UPI000CAC57D4, Uniprot reference A0A2K4ZN91.

[0648] Seq ID No. 500: Codon-optimized DNA encoding 6xHis Corynespora cassiicola glucosidase, Uniparc reference UPI000D237A4A, Uniprot reference A0A2T2NYD4.

[0649] Seq ID No. 501: Codon-optimized DNA encoding 6xHis Meira miltonrushii glucosidase, Uniparc reference UPI000D77C91D, Uniprot reference A0A316V6M3.

[0650] Seq ID No. 502: Codon-optimized DNA encoding 6xHis Bacteroides fragilis glucosidase, Uniparc reference UPI00004E1F76, Uniprot reference A0A380YVC7.

[0651] Seq ID No. 503: Codon-optimized DNA encoding 6xHis-restricted Malasseziae stricta glucosidase, Uniparc reference UPI000F0C30E8, Uniprot reference A0A3G2SB79.

[0652] Seq ID No. 504: Codon-optimized DNA encoding 6xHis Fusarium euwallaceae glucosidase, Uniparc reference UPI000FFFEFB2, Uniprot reference A0A430LYA2.

[0653] Seq ID No. 505: Codon-optimized DNA encoding 6xHis Psathyrella aberdarensis glucosidase, Uniparc reference UPI0010251887, Uniprot reference A0A4Q2E070.

[0654] Seq ID No. 506: Codon-optimized DNA encoding 6xHis Aeromonas hydrophila glucosidase, Uniparc reference UPI0000E69509, Uniprot reference A0KLP6.

[0655] Seq ID No. 507: Codon-optimized DNA encoding 6xHis Saccharopolyspora erythraea glucosidase, Uniparc reference UPI00000B86CB, Uniprot reference A4F7P9.

[0656] Seq ID No. 508: Codon-optimized DNA encoding 6xHis Streptomyces cessviceus glucosidase, Uniparc reference UPI000180240E, Uniprot reference B51181.

[0657] Seq ID No. 509: Codon-optimized DNA encoding 6xHis Naematelia encephala glucosidase, Uniparc reference UPI000A250F78, Uniprot reference A0A1Y2AWB7.

[0658] Seq ID No. 510: Codon-optimized DNA encoding 6xHis Hartmannibacter diazotrophicus glucosidase, Uniparc reference UPI000C0221F1, Uniprot reference A0A2C9D6I2.

[0659] Seq ID No. 511: Codon-optimized DNA encoding 6xHis Pontimonas salivibrio glucosidase, Uniparc reference UPI000CEB5AB1, Uniprot reference A0A2L2BPE2.

[0660] Seq ID No. 512: Codon-optimized DNA encoding 6xHis Cadophora sp. glucosidase, Uniparc reference UPI000D5B9C38, Uniprot reference A0A2V1CH24.

[0661] Seq ID No. 513: Codon-optimized DNA encoding 6xHis Meira miltonrushii glucosidase, Uniparc reference UPI000D779558, Uniprot reference A0A316V8S0.

[0662] Seq ID No. 514: Codon-optimized DNA encoding 6xHis Monilinia fructigena glucosidase, Uniparc reference UPI000DC42E3D, Uniprot reference A0A395IJW4.

[0663] Seq ID No. 515: Codon-optimized DNA encoding 6xHis Hortaea werneckii glucosidase, Uniparc reference UPI000F3E476C, Uniprot reference A0A3M6XGS0.

[0664] Seq ID No. 516: Codon-optimized DNA encoding 6xHis Streptomyces netropsis glucosidase, Uniparc reference UPI00101460D7, Uniprot reference A0A445N7U0.

[0665] Seq ID No. 517: Codon-optimized DNA encoding 6xHis Aureobasidium pullulans glucosidase, Uniparc reference UPI001139C6A8, Uniprot reference A0A4S9IFI0.

[0666] Seq ID No. 518: Codon-optimized DNA encoding 6xHis Aspergillus clavatus glucosidase, Uniparc reference UPI0000EA5CFF, Uniprot reference A1CTN9.

[0667] Seq ID No. 519: Codon-optimized DNA encoding 6xHis Clavibacter michiganensis glucosidase, Uniparc reference UPI0001523037, Uniprot reference A5CT94.

[0668] Seq ID No. 520: Codon-optimized DNA encoding 6xHis Penicillium rubens glucosidase, Uniparc reference UPI0001831CF5, Uniprot reference B6H7R5.

[0669] Seq ID No. 521: Codon-optimized DNA encoding 6xHis Lachnoclostridium sp. glucosidase, Uniparc reference UPI000B365547, Uniprot reference A0A1Y4NTL9.

[0670] Seq ID No. 522: Codon-optimized DNA encoding 6xHis Rhodobacteraceae bacterium glucosidase, Uniparc reference UPI000C09BF88, Uniprot reference A0A2D5IXB9.

[0671] Seq ID No. 523: Codon-optimized DNA encoding 6xHis Bacteroides fragilis glucosidase, Uniparc reference UPI0004B5EEF2, Uniprot reference A0A2M9UUC4.

[0672] Seq ID No. 524: Codon-optimized DNA encoding 6xHis Aspergillus indologenus glucosidase, Uniparc reference UPI000D7FE1ED, Uniprot reference A0A2V5IY78.

[0673] Seq ID No. 525: Codon-optimized DNA encoding 6xHis Acaromyces ingoldii glucosidase, Uniparc reference UPI000D802B25, Uniprot reference A0A316YR39.

[0674] Seq ID No. 526: Codon-optimized DNA encoding 6xHis Monilinia fructigena glucosidase, Uniparc reference UPI000DC60823, Uniprot reference A0A395J1U5.

[0675] Seq ID No. 527: Codon-optimized DNA encoding 6xHis xylanase Paenibacillus xylanexedens glucosidase, Uniparc reference UPI000F52D773, Uniprot reference A0A3N6CA02.

[0676] Seq ID No. 528: Codon-optimized DNA encoding 6xHis Actinomyceshowellii glucosidase, Uniparc reference UPI000F6DAAAE, Uniprot reference A0A448HIGO.

[0677] Seq ID No. 529: Codon-optimized DNA encoding 6xHis Friedmanniomyces endolithicus glucosidase, Uniparc reference UPI00113D19DF, Uniprot reference A0A4V5N9I4.

[0678] Seq ID No. 530: Codon-optimized DNA encoding 6xHis Neosartory afischii glucosidase, Uniparc reference UPI0000EA8672, Uniprot reference A1DNS0.

[0679] Seq ID No. 531: Codon-optimized DNA encoding 6xHis Pseudomonas aeruginosa glucosidase, Uniparc reference UPI0000D7314B, Uniprot reference A6V4K6.

[0680] Seq ID No. 532: Codon-optimized DNA encoding 6xHis Talaromycesstipitatus glucosidase, Uniparc reference UPI00018E7266, Uniprot reference B8MF24.

[0681] Seq ID No. 533: Codon-optimized DNA encoding 6xHis Aquimixticolasoesokkakensis glucosidase, Uniparc reference UPI000A1A5FD7, Uniprot reference A0A1Y5RVF8.

[0682] Seq ID No. 534: Codon-optimized DNA encoding 6xHis Rhodobacterales bacterium glucosidase, Uniparc reference UPI000C98D37C, Uniprot reference A0A2D9YGV1.

[0683] Seq ID No. 535: Codon-optimized DNA encoding 6xHis Methylorubrum extorquens glucosidase, Uniparc reference UPI0006F9793E, Uniprot reference A0A2N9AS40.

[0684] Seq ID No. 536: Codon-optimized DNA encoding 6xHis Clostridium perfringens glucosidase, Uniparc reference UPI00000CF7C8, Uniprot reference A0A2X2YBP0.

[0685] Seq ID No. 537: Codon-optimized DNA encoding 6xHis Acholeplasmatales bacterium glucosidase, Uniparc reference UPI0008AEBEA3, Uniprot reference A0A348NID6.

[0686] Seq ID No. 538: Codon-optimized DNA encoding 6xHis Bacteroidetesbacterium glucosidase, Uniparc reference UPI000EC3C979, Uniprot reference A0A3B8VHE8.

[0687] Seq ID No. 539: Codon-optimized DNA encoding 6xHis Clostridium carnis glucosidase, Uniparc reference UPI000F637E38, Uniprot reference A0A3 P6K8E8.

[0688] Seq ID No. 540: Codon-optimized DNA encoding 6xHis Mycolicibacterium flavescens glucosidase, Uniparc reference UPI000B93B5C9, Uniprot reference A0A448HNB5.

[0689] Seq ID No. 541: Codon-optimized DNA encoding 6xHis Streptococcus gallolyticus glucosidase, Uniparc reference UPI000F6EFA71, Uniprot reference A0A4V6LJ94.

[0690] Seq ID No. 542: Codon-optimized DNA encoding 6xHis Yersinia enterocolitica glucosidase, Uniparc reference UPI0000EB54CC, Uniprot reference A1JNB7.

[0691] Seq ID No. 543: Codon-optimized DNA encoding 6xHis Anaeromyxobacter sp. glucosidase, Uniparc reference UPI0000ED8A80, Uniprot reference A7HFG4.

[0692] Seq ID No. 544: Codon-optimized DNA encoding 6xHis Talaromycesstipitatus glucosidase, Uniparc reference UPI00018E7D70, Uniprot reference B8MK55.

[0693] Seq ID No. 545: Codon-optimized DNA encoding 6xHis Hortaea werneckii glucosidase, Uniparc reference UPI000A2E3FAA, Uniprot reference A0A1Z5SL14.

[0694] Seq ID No. 546: Codon-optimized DNA encoding 6xHis Micavibrio sp. glucosidase, Uniparc reference UPI000C529025, Uniprot reference A0A2E2Q8X2.

[0695] Seq ID No. 547: Codon-optimized DNA encoding 6xHis Acidobacteriia bacterial glucosidase, Uniparc reference UPI000CE6B996, Uniprot reference A0A2N9MBS0.

[0696] Seq ID No. 548: Codon-optimized DNA encoding 6xHis Corynebacterium jeikeium glucosidase, Uniparc reference UPI000DA3A972, Uniprot reference A0A2X4T570.

[0697] Seq ID No. 549: Codon-optimized DNA encoding 6xHis Clostridiaceae bacterium glucosidase, Uniparc reference UPI000E8D37A1, Uniprot reference A0A353PZH8.

[0698] Seq ID No. 550: Codon-optimized DNA encoding 6xHis glucosidase from Anaerolineaceae bacterium, Uniparc reference UPI000748C096, Uniprot reference A0A3B9PA35.

[0699] Seq ID No. 551: Codon-optimized DNA encoding 6xHis Gymnopilus dilepis glucosidase, Uniparc reference UPI000FF41956, Uniprot reference A0A409WSY0.

[0700] Seq ID No. 552: Codon-optimized DNA encoding 6xHis Kocuria rosea glucosidase, Uniparc reference UPI000F7105D4, Uniprot reference A0A448R8N0.

[0701] Seq ID No. 553: Codon-optimized DNA encoding 6xHis Teredinibacter sp. glucosidase, Uniparc reference UPI0011696FAB, Uniprot reference A0A509DWZ3.

[0702] Seq ID No. 554: Codon-optimized DNA encoding 6xHis Aspergillus niger glucosidase, Uniparc reference UPI0000EFB564, Uniprot reference A2QS42.

[0703] Seq ID No. 555: Codon-optimized DNA encoding 6xHis Laccaria bicolor glucosidase, Uniparc reference UPI000164423D, Uniprot reference B0D734.

[0704] Seq ID No. 556: Codon-optimized DNA encoding 6xHis Pedosphaera parvula glucosidase, Uniparc reference UPI00017357F6, Uniprot reference B9XH33.

[0705] Seq ID No. 557: Codon-optimized DNA encoding 6xHis Megamonas hypermegale glucosidase, Uniparc reference UPI00042469F6, Uniprot reference A0A239TGH2.

[0706] Seq ID No. 558: Codon-optimized DNA encoding 6xHis Armillaria gallica glucosidase, Uniparc reference UPI000BC209C3, Uniprot reference A0A2H3E300.

[0707] Seq ID No. 559: Codon-optimized DNA encoding 6xHis Micromonospora sp. glucosidase, Uniparc reference UPI000D2EAE87, Uniprot reference A0A2P8AV03.

[0708] Seq ID No. 560: Codon-optimized DNA encoding 6xHis Klebsiella oxytoca glucosidase, Uniparc reference UPI000DA286EE, Uniprot reference A0A2X5CJC5.

[0709] Seq ID No. 561: Codon-optimized DNA encoding 6xHis Candidatus ozemobacter glucosidase, Uniparc reference UPI000DFAEF6C, Uniprot reference A0A367ZIH8.

[0710] Seq ID No. 562: Codon-optimized DNA encoding 6xHis Coleophoma crateriformis glucosidase, Uniparc reference UPI000E399EE8, Uniprot reference A0A3D8R2C2.

[0711] Seq ID No. 563: Codon-optimized DNA encoding 6xHis Apiotrichum porosum glucosidase, Uniparc reference UPI000FBC01E3, Uniprot reference A0A427XHS2.

[0712] Seq ID No. 564: Codon-optimized DNA encoding 6xHis Acholeplasma hippikon glucosidase, Uniparc reference UPI00068E4E50, Uniprot reference A0A449BJ27.

[0713] Seq ID No. 565: Codon-optimized DNA encoding 6xHis Streptomyces spectabilis glucosidase, Uniparc reference UPI001185F074, Uniprot reference A0A516RGT1.

[0714] Seq ID No. 566: Codon-optimized DNA encoding 6xHis Aspergillus niger glucosidase, Uniparc reference UPI0000EFCED2, Uniprot reference A2R8G2.

[0715] Seq ID No. 567: Codon-optimized DNA encoding 6xHis Xanthomonas campestris glucosidase, Uniparc reference UPI00000D8BFA, Uniprot reference B0RYA0.

[0716] Seq ID No. 568: Codon-optimized DNA encoding 6xHis Lactobacillus paracasei glucosidase, Uniparc reference UPI00019C9CD7, Uniprot reference C2FDL2.

[0717] Seq ID No. 569: Codon-optimized DNA encoding 6xHis Bifiguratus adelaidae glucosidase, Uniparc reference UPI000BC490A3, Uniprot reference A0A261XUH4.

[0718] Seq ID No. 570: Codon-optimized DNA encoding 6xHis bacterial glucosidase, Uniparc reference UPI000CC31AE2, Uniprot reference A0A2H5YYA1.

[0719] Seq ID No. 571: Codon-optimized DNA encoding 6xHis Actinomadura parvosata glucosidase, Uniparc reference UPI000D26C9C6, Uniprot reference A0A2P9IY35.

[0720] Seq ID No. 572: Codon-optimized DNA encoding 6xHis Melissococcus plutonius glucosidase, Uniparc reference UPI00024F22B8, Uniprot reference A0A2Z5Y4P3.

[0721] Seq ID No. 573: Codon-optimized DNA encoding 6xHis Enterococcus durans glucosidase, Uniparc reference UPI000E020871, Uniprot reference A0A377KJS3.

[0722] Seq ID No. 574: Codon-optimized DNA encoding 6xHis-restricted Malasseziae stricta glucosidase, Uniparc reference UPI000DD17A7D, Uniprot reference A0A3G2S2J6.

[0723] Seq ID No. 575: Codon-optimized DNA encoding 6xHis Apiotrichum porosum glucosidase, Uniparc reference UPI000FA2DE87, Uniprot reference A0A427XZQ0.

[0724] Seq ID No. 576: Codon-optimized DNA encoding 6xHis Streptomonospora sp. glucosidase, Uniparc reference UPI0010355193, Uniprot reference A0A4P6PWP5.

[0725] Seq ID No. 577: Codon-optimized DNA encoding 6xHis Lactobacillus gasseri glucosidase, Uniparc reference UPI00119640A1, Uniprot reference A0A558LH47.

[0726] Seq ID No. 578: Codon-optimized DNA encoding 6xHis Aspergillus niger glucosidase, Uniparc reference UPI0000EFD0AA, Uniprot reference A2RAJ1.

[0727] Seq ID No. 579: Codon-optimized DNA encoding 6xHis Neosartorya fumigata glucosidase, Uniparc reference UPI000170BF91, Uniprot reference B0XXG1.

[0728] Seq ID No. 580: Codon-optimized DNA encoding 6xHis fastidious Eubacterium eligens glucosidase, Uniparc reference UPI0001A5B234, Uniprot reference C4Z6T5.

[0729] Seq ID No. 581: Codon-optimized DNA encoding 6xHis Bifiguratus adelaidae glucosidase, Uniparc reference UPI000BC64A75, Uniprot reference A0A261XVM8.

[0730] Seq ID No. 582: Codon-optimized DNA encoding 6xHis bacterial glucosidase, Uniparc reference UPI000CAA854E, Uniprot reference A0A2H6EX57.

[0731] Seq ID No. 583: Codon-optimized DNA encoding 6xHis Corynespora cassiicola glucosidase, Uniparc reference UPI000D24EC2C, Uniprot reference A0A2T2N5H9.

[0732] Seq ID No. 584: Codon-optimized DNA encoding 6xHis Pseudomicrostroma glucosiphilum glucosidase, Uniparc reference UPI000D77A0B7, Uniprot reference A0A316UK83.

[0733] Seq ID No. 585: Codon-optimized DNA encoding 6xHis Staphylococcus saprophyticus glucosidase, Uniparc reference UPI0002DAAFCC, Uniprot reference A0A380HD31.

[0734] Seq ID No. 586: Codon-optimized DNA encoding a 6xHis-restricted Malasseziae stricta glucosidase, Uniparc reference UPI000F0C6B8E, Uniprot reference A0A3G2S932.

[0735] Seq ID No. 587: Codon-optimized DNA encoding 6xHis Saitozyma podzolica glucosidase, Uniparc reference UPI000FBA70E2, Uniprot reference A0A427YCL1.

[0736] Seq ID No. 588: Codon-optimized DNA encoding 6xHis Tremella mesenterica glucosidase, Uniparc reference UPI00102822B4, Uniprot reference A0A4Q1BRC6.

[0737] Seq ID No. 589: Codon-optimized DNA encoding 6xHis Arthrobacter sp. glucosidase, Uniparc reference UPI0000527506, Uniprot reference A0JZ86.

[0738] Seq ID No. 590: Codon-optimized DNA encoding 6xHis Scheffersomyces stipitis glucosidase, Uniparc reference UPI000157388C, Uniprot reference A3LRB0.

[0739] Seq ID No. 591: Codon-optimized DNA encoding 6xHis Leptothrix cholodnii glucosidase, Uniparc reference UPI0001712E02, Uniprot reference B1XZK8.

[0740] Seq ID No. 592: Codon-optimized DNA encoding 6xHis Thauera sp. glucosidase, Uniparc reference UPI000166883C, Uniprot reference C4ZLL7. Seq ID No. 593: Codon-optimized DNA encoding 6xHis Kosmotoga olearia glucosidase, Uniparc reference UPI00018494AB, Uniprot reference C5CDW5.

[0741] Seq ID No. 594: Codon-optimized DNA encoding 6xHis Roseburia intestinalis glucosidase, Uniparc reference UPI0001CD671F, Uniprot reference D4L3Y2.

[0742] Seq ID No. 595: Codon-optimized DNA encoding 6xHis Streptococcus equinus glucosidase, Uniparc reference UPI0001E0DC00, Uniprot reference E0PDF8.

[0743] Seq ID No. 596: Codon-optimized DNA encoding 6xHis Streptococcus cristatus glucosidase, Uniparc reference UPI0001F80C4B, Uniprot reference E8JUK5.

[0744] Seq ID No. 597: Codon-optimized DNA encoding 6xHis Cellulosilyticum lentocellum glucosidase, Uniparc reference UPI0001D2DBBC, Uniprot reference F2JLH3.

[0745] Seq ID No. 598: Codon-optimized DNA encoding 6xHis Streptococcus gallolyticus glucosidase, Uniparc reference UPI0001C48657, Uniprot reference F5WYI5.

[0746] Seq ID No. 599: Codon-optimized DNA encoding 6xHis Ketogulonicigenium vulgare glucosidase, Uniparc reference UPI00021D443A, Uniprot reference F9Y8Z7.

[0747] Seq ID No. 600: Codon-optimized DNA encoding 6xHis Spathaspora passalidarum glucosidase, Uniparc reference UPI000228255D, Uniprot reference G3AGX1.

[0748] Seq ID No. 601: Codon-optimized DNA encoding 6xHis Niastella koreensis glucosidase, Uniparc reference UPI00023F6F5F, Uniprot reference G8T9J3.

[0749] Seq ID No. 602: Codon-optimized DNA encoding 6xHis Cellvibrio sp. glucosidase, Uniparc reference UPI000260108C, Uniprot reference 13IDC0.

[0750] Seq ID No. 603: Codon-optimized DNA encoding 6xHis Flavobacterium sp. glucosidase, Uniparc reference UPI000272D1E0, Uniprot reference J1ACA0.

[0751] Seq ID No. 604: Codon-optimized DNA encoding 6xHis Macrophomina phaseolina glucosidase, Uniparc reference UPI00028E7FE1, Uniprot reference K2S5D3.

[0752] Seq ID No. 605: Codon-optimized DNA encoding 6xHis Kosmotoga olearia glucosidase, Uniparc reference UPI00018483A2, Uniprot reference C5CHI5.

[0753] Seq ID No. 606: Codon-optimized DNA encoding 6xHis Blautia obeum glucosidase, Uniparc reference UPI0001CD5918, Uniprot reference D4LRF6.

[0754] Seq ID No. 607: Codon-optimized DNA encoding 6xHis Bifidobacterium dentium glucosidase, Uniparc reference UPI0001E18CDA, Uniprot reference E0Q541.

[0755] Seq ID No. 608: Codon-optimized DNA encoding 6xHis Anaerolineathermophila glucosidase, Uniparc reference UPI0001F55F8F, Uniprot reference E8N5R8.

[0756] Seq ID No. 609: Codon-optimized DNA encoding 6xHis Coriobacterium glomerans glucosidase, Uniparc reference UPI0002050DA2, Uniprot reference F2N7E4.

[0757] Seq ID No. 610: Codon-optimized DNA encoding 6xHis Microlunatus phosphovorus glucosidase, Uniparc reference UPI000210C886, Uniprot reference F5XJQ3.

[0758] Seq ID No. 611: Codon-optimized DNA encoding 6xHis Streptomyces sp. glucosidase, Uniparc reference UPI0001C18877, Uniprot reference G0Q1U8.

[0759] Seq ID No. 612: Codon-optimized DNA encoding 6xHis Spathaspora passalidarum glucosidase, Uniparc reference UPI0002282B61, Uniprot reference G3AIV6.

[0760] Seq ID No. 613: Codon-optimized DNA encoding 6xHis Glarealozoyensis glucosidase, Uniparc reference UPI0002402F4A, Uniprot reference H0ET34.

[0761] Seq ID No. 614: Codon-optimized DNA encoding 6xHis Glaciozyma antarctica glucosidase, Uniparc reference UPI0002633B36, Uniprot reference I3UJK0.

[0762] Seq ID No. 615: Codon-optimized DNA encoding 6xHis Arthrobacter sp. glucosidase, Uniparc reference UPI00027DFD35, Uniprot reference J7LN00.

[0763] Seq ID No. 616: Codon-optimized DNA encoding 6xHis Fusariumpseudograminearum glucosidase, Uniparc reference UPI00028D698C, Uniprot reference K3VMA9.

[0764] Seq ID No. 617: Codon-optimized DNA encoding 6xHis Hypocrea rufa glucosidase, Uniparc reference UPI000006AA61, Uniprot reference C6GGC9.

[0765] Seq ID No. 618: Codon-optimized DNA encoding 6xHis Ruminococcus torques glucosidase, Uniparc reference UPI0001CDA964, Uniprot reference D4M6W6.

[0766] Seq ID No. 619: Codon-optimized DNA encoding 6xHis Bifidobacterium dentium glucosidase, Uniparc reference UPI0001E17370, Uniprot reference E0Q9Z7.

[0767] Seq ID No. 620: Codon-optimized DNA encoding 6xHis Bacteroides salanitronis glucosidase, Uniparc reference UPI0001FC71F6, Uniprot reference F0R2D7.

[0768] Seq ID No. 621: Codon-optimized DNA encoding 6xHis Bacteroides coprosuis glucosidase, Uniparc reference UPI00020E6D19, Uniprot reference F3ZQ40.

[0769] Seq ID No. 622: Codon-optimized DNA encoding 6xHis Marinomonas posidonica glucosidase, Uniparc reference UPI00020D4CC6, Uniprot reference F6CWF6.

[0770] Seq ID No. 623: Codon-optimized DNA encoding 6xHis Chaetomium thermophilum glucosidase, Uniparc reference UPI000227E8ED, Uniprot reference G0SE64.

[0771] Seq ID No. 624: Codon-optimized DNA encoding 6xHis Tetragenococcus halophilus glucosidase, Uniparc reference UPI00022B9A15, Uniprot reference G4L5K1.

[0772] Seq ID No. 625: Codon-optimized DNA encoding 6xHis Paenibacillus sp. glucosidase, Uniparc reference UPI00024F0867, Uniprot reference H6CCZ8.

[0773] Seq ID No. 626: Codon-optimized DNA encoding 6xHis Turneriella parva glucosidase, Uniparc reference UPI000265AA4B, Uniprot reference I4B8U7.

[0774] Seq ID No. 627: Codon-optimized DNA encoding 6xHis Arthrobacter sp. glucosidase, Uniparc reference UPI00027DFDB2, Uniprot reference J7LQK9.

[0775] Seq ID No. 628: Codon-optimized DNA encoding 6xHis Agaricus bisporus glucosidase, Uniparc reference UPI00029074B1, Uniprot reference K5W7V1.

[0776] Seq ID No. 629: Codon-optimized DNA encoding 6xHis glucosidase from the flagellate Nectria haematococca, Uniparc reference UPI0001B67634, Uniprot reference C7YIP3.

[0777] Seq ID No. 630: Codon-optimized DNA encoding 6xHis Bacteroides xylanisolvens glucosidase, Uniparc reference UPI0001A25287, Uniprot reference D4VSZ0.

[0778] Seq ID No. 631: Codon-optimized DNA encoding 6xHis Sediminispirochaetas maragdinae glucosidase, Uniparc reference UPI0001DD9790, Uniprot reference E1R331.

[0779] Seq ID No. 632: Codon-optimized DNA encoding 6xHis Deinococcus proteolyticus glucosidase, Uniparc reference UPI0001FC42E9, Uniprot reference F0RPV2.

[0780] Seq ID No. 633: Codon-optimized DNA encoding 6xHis Sphingobacterium sp. glucosidase, Uniparc reference UPI0002033A0A, Uniprot reference F4C226.

[0781] Seq ID No. 634: Codon-optimized DNA encoding 6xHis Sphingobium chlorophenolicum glucosidase, Uniparc reference UPI0001E54133, Uniprot reference F6ET40.

[0782] Seq ID No. 635: Codon-optimized DNA encoding 6xHis Caloramator australicus glucosidase, Uniparc reference UPI00021CACC4, Uniprot reference G0V3V5.

[0783] Seq ID No. 636: Codon-optimized DNA encoding 6xHis Commensalibacter intestini glucosidase, Uniparc reference UPI000230E3BF, Uniprot reference G6F370.

[0784] Seq ID No. 637: Codon-optimized DNA encoding 6xHis Paenibacillus sp. glucosidase, Uniparc reference UPI00024F07AC, Uniprot reference H6CIT2.

[0785] Seq ID No. 638: Codon-optimized DNA encoding 6xHis Nitrolancea hollandica glucosidase, Uniparc reference UPI0002638AF3, Uniprot reference I4EIA9.

[0786] Seq ID No. 639: Codon-optimized DNA encoding 6xHis Cryptococcus neoformans glucosidase, Uniparc reference UPI000392C3ED, Uniprot reference J9VVK7.

[0787] Seq ID No. 640: Codon-optimized DNA encoding 6xHis Acidipropionibacterium acidipropionici glucosidase, Uniparc reference UPI0002988588, Uniprot reference K7S596.

[0788] Seq ID No. 641: Codon-optimized DNA encoding 6xHis Prevotella sp. glucosidase, Uniparc reference UPI0001B93465, Uniprot reference C9PT75.

[0789] Seq ID No. 642: Codon-optimized DNA encoding 6xHis Rhodobacter capsulatus glucosidase, Uniparc reference UPI0001D08095, Uniprot reference D5ALU0.

[0790] Seq ID No. 643: Codon-optimized DNA encoding 6xHis Stigmatella aurantiaca glucosidase, Uniparc reference UPI0001E74370, Uniprot reference E3FJ05.

[0791] Seq ID No. 644: Codon-optimized DNA encoding 6xHis Sphaerochaeta globosa glucosidase, Uniparc reference UPI0002010060, Uniprot reference F0RVK3.

[0792] Seq ID No. 645: Codon-optimized DNA encoding 6xHis Sphaerochaetacoccoides glucosidase, Uniparc reference UPI000207D78C, Uniprot reference F4GH96.

[0793] Seq ID No. 646: Codon-optimized DNA encoding 6xHis Novosphingobium sp. glucosidase, Uniparc reference UPI00020EFBCB, Uniprot reference F6ICQ5.

[0794] Seq ID No. 647: Codon-optimized DNA encoding 6xHis Arthrobotrysoligospora glucosidase, Uniparc reference UPI000225331F, Uniprot reference G1XH86.

[0795] Seq ID No. 648: Codon-optimized DNA encoding 6xHis Lactococcus lactis glucosidase, Uniparc reference UPI00000C6907, Uniprot reference G6FFS4.

[0796] Seq ID No. 649: Codon-optimized DNA encoding 6xHis Phaeospirillum molischianum glucosidase, Uniparc reference UPI000255313A, Uniprot reference H8FXH7.

[0797] Seq ID No. 650: Codon-optimized DNA encoding 6xHis Marinoptera glucosidase, Uniparc reference UPI0002609C68, Uniprot reference I4EW72.

[0798] Seq ID No. 651: Codon-optimized DNA encoding 6xHis Saccharothrix espanaensis glucosidase, Uniparc reference UPI00028AC337, Uniprot reference K0K125.

[0799] Seq ID No. 652: Codon-optimized DNA encoding 6xHis Cronobacter sakazakii glucosidase, Uniparc reference UPI00029BA293, Uniprot reference K8DAK0.

[0800] Seq ID No. 653: Codon-optimized DNA encoding 6xHis Verticillium alfalfae glucosidase, Uniparc reference UPI0001BBDF1E, Uniprot reference C9SVX1.

[0801] Seq ID No. 654: Codon-optimized DNA encoding 6xHis Bacteroides xylanisolvens glucosidase, Uniparc reference UPI0001CCFD36, Uniprot reference D6CY10.

[0802] Seq ID No. 655: Codon-optimized DNA encoding 6xHis Leadbetterella byssophila glucosidase, Uniparc reference UPI0001EBD98A, Uniprot reference E4RUR0.

[0803] Seq ID No. 656: Codon-optimized DNA encoding 6xHis Sphaerochaeta globosa glucosidase, Uniparc reference UPI00020102A5, Uniprot reference F0RYB6.

[0804] Seq ID No. 657: Codon-optimized DNA encoding 6xHis Sphaerochaetacoccoides glucosidase, Uniparc reference UPI000207D6A0, Uniprot reference F4GLH6.

[0805] Seq ID No. 658: Codon-optimized DNA encoding 6xHis Haloplasma contractile glucosidase, Uniparc reference UPI000212252C, Uniprot reference F7Q0Y2.

[0806] Seq ID No. 659: Codon-optimized DNA encoding 6xHis Nitrospirillum amazonense glucosidase, Uniparc reference UPI0002265447, Uniprot reference G1Y4C7.

[0807] Seq ID No. 660: Codon-optimized DNA encoding 6xHis Azospirillum brasilense glucosidase, Uniparc reference UPI00023420B8, Uniprot reference G8AWD9.

[0808] Seq ID No. 661: Codon-optimized DNA encoding 6xHis Phaeospirillum molischianum glucosidase, Uniparc reference UPI000255314F, Uniprot reference H8FXJ8.

[0809] Seq ID No. 662: Codon-optimized DNA encoding 6xHis Marinoptera glucosidase, Uniparc reference UPI000260A2FA, Uniprot reference I4EYD5.

[0810] Seq ID No. 663: Codon-optimized DNA encoding 6xHis Wickerhamomyces ciferrii (Wickerhamomyces ciferrii) glucosidase, Uniparc reference UPI000283EB8D, Uniprot reference K0KVJ2.

[0811] Seq ID No. 664: Codon-optimized DNA encoding 6xHis Gloeocapsa sp. glucosidase, Uniparc reference UPI0002A5D085, Uniprot reference K9XKL8.

[0812] Seq ID No. 665: Codon-optimized DNA encoding 6xHis Sphaerobacter thermophilus glucosidase, Uniparc reference UPI0001A3BCB6, Uniprot reference D1C7U8.

[0813] Seq ID No. 666: Codon-optimized DNA encoding 6xHis Bacteroides xylanisolvens glucosidase, Uniparc reference UPI0001CCEF1F, Uniprot reference D6D4V2.

[0814] Seq ID No. 667: Codon-optimized DNA encoding 6xHis Prevotella buccae glucosidase, Uniparc reference UPI0001F149E8, Uniprot reference E6K4W5.

[0815] Seq ID No. 668: Codon-optimized DNA encoding 6xHis Grosmannia clavigera glucosidase, Uniparc reference UPI0001FF1101, Uniprot reference FOXBR0.

[0816] Seq ID No. 669: Codon-optimized DNA encoding 6xHis Melampsora larici-populina glucosidase, Uniparc reference UPI00020F9774, Uniprot reference F4R4W2.

[0817] Seq ID No. 670: Codon-optimized DNA encoding 6xHis Prevotella multisaccharivorax glucosidase, Uniparc reference UPI0002138E9C, Uniprot reference F8N7G1.

[0818] Seq ID No. 671: Codon-optimized DNA encoding 6xHis Streptomyces zinciresistens glucosidase, Uniparc reference UPI0002255A63, Uniprot reference G2G8K4.

[0819] Seq ID No. 672: Codon-optimized DNA encoding 6xHis Granulicella mallensis glucosidase, Uniparc reference UPI0001D9FC40, Uniprot reference G8NY42.

[0820] Seq ID No. 673: Codon-optimized DNA encoding 6xHis Gibberella zeae glucosidase, Uniparc reference UPI00021F1FEE, Uniprot reference I1RH94.

[0821] Seq ID No. 674: Codon-optimized DNA encoding 6xHis Marinoptera glucosidase, Uniparc reference UPI00026090A2, Uniprot reference I4EYK6.

[0822] Seq ID No. 675: Codon-optimized DNA encoding 6xHis Lactobacillus equicursoris glucosidase, Uniparc reference UPI0002869F55, Uniprot reference K0NRS8.

[0823] Seq ID No. 676: Codon-optimized DNA encoding 6xHis Colletotrichum fructicola glucosidase, Uniparc reference UPI0002A93280, Uniprot reference L2F9W0.

[0824] Seq ID No. 677: Codon-optimized DNA encoding 6xHis Streptosporangium roseum glucosidase, Uniparc reference UPI0001BF8AF6, Uniprot reference D2B261.

[0825] Seq ID No. 678: Codon-optimized DNA encoding 6xHis Listeria grayi glucosidase, Uniparc reference UPI00019F252A, Uniprot reference D7UX19.

[0826] Seq ID No. 679: Codon-optimized DNA encoding 6xHis Enterococcus italicus glucosidase, Uniparc reference UPI0001F11EFF, Uniprot reference E6LF07.

[0827] Seq ID No. 680: Codon-optimized DNA encoding 6xHis Fluviicola taffensis glucosidase, Uniparc reference UPI000203D9EA, Uniprot reference F2IIT6.

[0828] Seq ID No. 681: Codon-optimized DNA encoding 6xHis Shigella flexneri glucosidase, Uniparc reference UPI00020CA641, Uniprot reference F5N4W9.

[0829] Seq ID No. 682: Codon-optimized DNA encoding 6xHis Actinomyces sp. glucosidase, Uniparc reference UPI0002189080, Uniprot reference F9EFW5.

[0830] Seq ID No. 683: Codon-optimized DNA encoding 6xHis Verticillium dahliae glucosidase, Uniparc reference UPI00022EBE5A, Uniprot reference G2X5V6.

[0831] Seq ID No. 684: Codon-optimized DNA encoding 6xHis Actinoplanes sp. glucosidase, Uniparc reference UPI00023ED5A8, Uniprot reference G8S0M0.

[0832] Seq ID No. 685: Codon-optimized DNA encoding 6xHis Gibberella zeae glucosidase, Uniparc reference UPI000023EA29, Uniprot reference I1S320.

[0833] Seq ID No. 686: Codon-optimized DNA encoding 6xHis Auricularia subglabra glucosidase, Uniparc reference UPI00027CE685, Uniprot reference J0WV06.

[0834] Seq ID No. 687: Codon-optimized DNA encoding 6xHis Nitratireductor indicus glucosidase, Uniparc reference UPI00028E86D6, Uniprot reference K2N268.

[0835] Seq ID No. 688: Codon-optimized DNA encoding 6xHis Thermoclostridium stercorarium glucosidase, Uniparc reference UPI0002AD999E, Uniprot reference L7VH66.

[0836] Seq ID No. 689: Codon-optimized DNA encoding 6xHis Geobacillus sp. glucosidase, Uniparc reference UPI0002AF2DE3, Uniprot reference L8A1S2.

[0837] Seq ID No. 690: Codon-optimized DNA encoding 6xHis uncultured bacterial glucosidase, Uniparc reference UPI000327C3CC, Uniprot reference M9ZC55.

[0838] Seq ID No. 691: Codon-optimized DNA encoding 6xHis Burkholderia ambifaria glucosidase, Uniparc reference UPI000059ACE1, Uniprot reference Q0BAK2.

[0839] Seq ID No. 692: Codon-optimized DNA encoding 6xHis Aspergillus oryzae glucosidase, Uniparc reference UPI0000676B8D, Uniprot reference Q2UIR4.

[0840] Seq ID No. 693: Codon-optimized DNA encoding 6xHis Yersinia pseudotuberculosis glucosidase, Uniparc reference UPI00004269B5, Uniprot reference Q66DJ0.

[0841] Seq ID No. 694: Codon-optimized DNA encoding 6xHis Wallemia ichthyophaga glucosidase, Uniparc reference UPI000331CC98, Uniprot reference R9AF64.

[0842] Seq ID No. 695: Codon-optimized DNA encoding 6xHis Glarealozoyensis glucosidase, Uniparc reference UPI0003522404, Uniprot reference S3DIM6.

[0843] Seq ID No. 696: Codon-optimized DNA encoding 6xHis Moniliophthora aroreri glucosidase, Uniparc reference UPI0003BF753A, Uniprot reference V2YU31.

[0844] Seq ID No. 697: Codon-optimized DNA encoding 6xHis Zhouia amylolytica glucosidase, Uniparc reference UPI0003DBCB4F, Uniprot reference W2ULL3.

[0845] Seq ID No. 698: Codon-optimized DNA encoding 6xHis Fusarium oxysporum glucosidase, Uniparc reference UPI0003F362FF, Uniprot reference W9NQQ3.

[0846] Seq ID No. 699: Codon-optimized DNA encoding 6xHis Flavobacterium johnsoniae glucosidase, Uniparc reference UPI00006EA168, Uniprot reference A5FAA5.

[0847] Seq ID No. 700: Codon-optimized DNA encoding 6xHis rice (Oryza sativa) glucosidase, Uniparc reference UPI00002394F5, Uniprot reference Q75193.

[0848] Seq ID No. 701: Codon-optimized DNA encoding 6xHis Clostridium saccharoperbutylacetonicum glucosidase, Uniparc reference UPI0002B65681, Uniprot reference M1MCD1.

[0849] Seq ID No. 702: Codon-optimized DNA encoding 6xHis Rhodococcus sp. glucosidase, Uniparc reference UPI0002D21DB1, Uniprot reference N1MBN6.

[0850] Seq ID No. 703: Codon-optimized DNA encoding 6xHis Burkholderia ambifaria glucosidase, Uniparc reference UPI000059C0CD, Uniprot reference Q0BCV8.

[0851] Seq ID No. 704: Codon-optimized DNA encoding 6xHis Xanthomonas campestris glucosidase, Uniparc reference UPI00005CE9E7, Uniprot reference Q3BVH7.

[0852] Seq ID No. 705: Codon-optimized DNA encoding 6xHis Caulobacter vibrioides glucosidase, Uniparc reference UPI00000C7604, Uniprot reference Q9A6F8.

[0853] Seq ID No. 706: Codon-optimized DNA encoding 6xHis Arcticibacter svalbardensis glucosidase, Uniparc reference UPI000338772A, Uniprot reference R9GRA7.

[0854] Seq ID No. 707: Codon-optimized DNA encoding 6xHis Winogradskyella psychrotolerans glucosidase, Uniparc reference UPI00035A4604, Uniprot reference S7VQ28.

[0855] Seq ID No. 708: Codon-optimized DNA encoding 6xHis Methyloglobulus morosus glucosidase, Uniparc reference UPI0003C4ED6A, Uniprot reference V5DXT8.

[0856] Seq ID No. 709: Codon-optimized DNA encoding 6xHis Pestalotiopsis fici glucosidase, Uniparc reference UPI0003E05C04, Uniprot reference W3WV37.

[0857] Seq ID No. 710: Codon-optimized DNA encoding 6xHis Capronia coronata glucosidase, Uniparc reference UPI000434E698, Uniprot reference W9YNR7.

[0858] Seq ID No. 711: Codon-optimized DNA encoding 6xHis Aspergillus aculeatus glucosidase, Uniparc reference UPI00001268FD, Uniprot reference P48825.

[0859] Seq ID No. 712: Codon-optimized DNA encoding 6xHis Thermotoganea politana glucosidase, Uniparc reference UPI0000DD5996, Uniprot reference Q0GC07.

[0860] Seq ID No. 713: Codon-optimized DNA encoding 6xHis Clostridium saccharoperbutylacetonicum glucosidase, Uniparc reference UPI0002B666E8, Uniprot reference M1MJF4.

[0861] Seq ID No. 714: Codon-optimized DNA encoding 6xHis Schizosaccharomyces pombe glucosidase, Uniparc reference UPI000006B001, Uniprot reference O74799.

[0862] Seq ID No. 715: Codon-optimized DNA encoding 6xHis Phaeosphaerianodorum glucosidase, Uniparc reference UPI000161BD2B, Uniprot reference Q0TXF6.

[0863] Seq ID No. 716: Codon-optimized DNA encoding 6xHis Xylella fastidiosa Dixon glucosidase, Uniparc reference UPI00003806C8, Uniprot reference Q3RGJ3.

[0864] Seq ID No. 717: Codon-optimized DNA encoding 6xHis Schizosaccharomyces pombe glucosidase, Uniparc reference UPI000006A330, Uniprot reference Q9P6J6.

[0865] Seq ID No. 718: Codon-optimized DNA encoding 6xHis Arcticibacter svalbardensis glucosidase, Uniparc reference UPI000337E887, Uniprot reference R9GWD6.

[0866] Seq ID No. 719: Codon-optimized DNA encoding 6xHis Colletotrichum gloeosporioides glucosidase, Uniparc reference UPI0003885717, Uniprot reference T0KJI7.

[0867] Seq ID No. 720: Codon-optimized DNA encoding 6xHis uncultured bacterial glucosidase, Uniparc reference UPI0003C9E340, Uniprot reference V5R1E8.

[0868] Seq ID No. 721: Codon-optimized DNA encoding 6xHis Xanthomonas arboricola glucosidase, Uniparc reference UPI0003E06A61, Uniprot reference W4S7I5.

[0869] Seq ID No. 722: Codon-optimized DNA encoding 6xHis Fusarium oxysporum glucosidase, Uniparc reference UPI00021EC697, Uniprot reference X0A8X8.

[0870] Seq ID No. 723: Codon-optimized DNA encoding 6xHis Paenibacillus polymyxa glucosidase, Uniparc reference UPI00001108D0, Uniprot reference P22073.

[0871] Seq ID No. 724: Codon-optimized DNA encoding 6xHis Kluyveromyces marxianus glucosidase, Uniparc reference UPI0001BE5ADA, Uniprot reference D1GCC6.

[0872] Seq ID No. 725: Codon-optimized DNA encoding 6xHis Ilumatobacter coccineus glucosidase, Uniparc reference UPI0002C04A25, Uniprot reference M5A594.

[0873] Seq ID No. 726: Codon-optimized DNA encoding 6xHis Agrobacterium sp. glucosidase, Uniparc reference UPI0000126912, Uniprot reference P12614.

[0874] Seq ID No. 727: Codon-optimized DNA encoding 6xHis Cytophaga hutchinsonii glucosidase, Uniparc reference UPI000038ECF7, Uniprot reference Q11P53.

[0875] Seq ID No. 728: Codon-optimized DNA encoding 6xHis Thermobifida fusca glucosidase, Uniparc reference UPI00003C5CA4, Uniprot reference Q47PF5.

[0876] Seq ID No. 729: Codon-optimized DNA encoding 6xHis Botryotinia fuckeliana glucosidase, Uniparc reference UPI0000069E5E, Uniprot reference Q9UVJ6.

[0877] Seq ID No. 730: Codon-optimized DNA encoding 6xHis Agarivorans albus glucosidase, Uniparc reference UPI00033994D2, Uniprot reference R9PTK2.

[0878] Seq ID No. 731: Codon-optimized DNA encoding 6xHis Enterococcus sp. glucosidase, Uniparc reference UPI00038B7639, Uniprot reference TOUDU2.

[0879] Seq ID No. 732: Codon-optimized DNA encoding 6xHis Salinispira pacifica glucosidase, Uniparc reference UPI0003D8A7DC, Uniprot reference V5WKT4.

[0880] Seq ID No. 733: Codon-optimized DNA encoding 6xHis Bacteroides xylanisolvens glucosidase, Uniparc reference UPI0003ECF15E, Uniprot reference W6P696.

[0881] Seq ID No. 734: Codon-optimized DNA encoding 6xHis Fusarium oxysporum glucosidase, Uniparc reference UPI000430029A, Uniprot reference X0AB46.

[0882] Seq ID No. 735: Codon-optimized DNA encoding 6xHis Thermotoga maritima glucosidase, Uniparc reference UPI0000126906, Uniprot reference Q08638B.

[0883] Seq ID No. 736: Codon-optimized DNA encoding 6xHis Neotermes koshunensis glucosidase, Uniparc reference UPI0000083EA8, Uniprot reference Q8T0W7.

[0884] Seq ID No. 737: Codon-optimized DNA encoding 6xHis Thanatephorus cucumeris glucosidase, Uniparc reference UPI0002BF34B3, Uniprot reference M5CHG9.

[0885] Seq ID No. 738: Codon-optimized DNA encoding 6xHis Hungateiclostridium thermocellum glucosidase, Uniparc reference UPI000053581D, Uniprot reference P14002.

[0886] Seq ID No. 739: Codon-optimized DNA encoding 6xHis Koribacter versatilis glucosidase, Uniparc reference UPI0000D76A70, Uniprot reference Q1IJ89.

[0887] Seq ID No. 740: Codon-optimized DNA encoding 6xHis Neosartorya fumigata glucosidase, Uniparc reference UPI00005203D8, Uniprot reference Q4WLX5.

[0888] Seq ID No. 741: Codon-optimized DNA encoding 6xHis vancomycin Amycolatopsis vancoresmycina glucosidase, Uniparc reference UPI00032DB8C1, Uniprot reference R1FKF0.

[0889] Seq ID No. 742: Codon-optimized DNA encoding 6xHis Gibberella fujikuroi glucosidase, Uniparc reference UPI0003519C76, Uniprot reference S0EKU1.

[0890] Seq ID No. 743: Codon-optimized DNA encoding 6xHis Bifidobacterium longum glucosidase, Uniparc reference UPI000390F839, Uniprot reference T2I2H5.

[0891] Seq ID No. 744: Codon-optimized DNA encoding 6xHis uncultured bacterial glucosidase, Uniparc reference UPI0003DFF0DA, Uniprot reference W0FLD1.

[0892] Seq ID No. 745: Codon-optimized DNA encoding 6xHis Bacteroides xylanisolvens glucosidase, Uniparc reference UPI0002D3D994, Uniprot reference W6P9J9.

[0893] Seq ID No. 746: Codon-optimized DNA encoding 6xHis Fusarium oxysporum glucosidase, Uniparc reference UPI00042F8016, Uniprot reference X0HA35.

[0894] Seq ID No. 747: Codon-optimized DNA encoding 6xHis Sorghum bicolor glucosidase, Uniparc reference UPI00000A7F40, Uniprot reference Q41290.

[0895] Seq ID No. 748: Codon-optimized DNA encoding 6xHis Clostridium cellulovorans glucosidase, Uniparc reference UPI000050B701, Uniprot reference Q53EH2.

[0896] Seq ID No. 749: Codon-optimized DNA encoding 6xHis Dacryopinax primogenitus glucosidase, Uniparc reference UPI0002C29EAA, Uniprot reference M5G1U5.

[0897] Seq ID No. 750: Codon-optimized DNA encoding 6xHis Rhizobium radiobacter glucosidase, Uniparc reference UPI0000126913, Uniprot reference P27034.

[0898] Seq ID No. 751: Codon-optimized DNA encoding 6xHis Phanerochaetechrysosporium glucosidase, Uniparc reference UPI00006E0CC7, Uniprot reference Q25BW5A.

[0899] Seq ID No. 752: Codon-optimized DNA encoding 6xHis Enterobacter agglomerans glucosidase, Uniparc reference UPI0000126905, Uniprot reference Q59437.

[0900] Seq ID No. 753: Codon-optimized DNA encoding 6xHis Candidatus microthrix glucosidase, Uniparc reference UPI00033056C4, Uniprot reference R4YWU4.

[0901] Seq ID No. 754: Codon-optimized DNA encoding 6xHis Chthonomonas calidirosea glucosidase, Uniparc reference UPI0003427F29, Uniprot reference S0ET37.

[0902] Seq ID No. 755: Codon-optimized DNA encoding 6xHis Acholeplasma brassicae glucosidase, Uniparc reference UPI0003B04208, Uniprot reference U4KMR7.

[0903] Seq ID No. 756: Codon-optimized DNA encoding 6xHis Chania multitudinisentens glucosidase, Uniparc reference UPI0003E13BD3, Uniprot reference W0LJ28.

[0904] Seq ID No. 757: Codon-optimized DNA encoding 6xHis Gibberella moniliformis glucosidase, Uniparc reference UPI0003ECCF80, Uniprot reference W7MW98.

[0905] Seq ID No. 758: Codon-optimized DNA encoding 6xHis Fusarium oxysporum glucosidase, Uniparc reference UPI0004305856, Uniprot reference X0I2U8.

[0906] Seq ID No. 759: Codon-optimized DNA encoding 6xHis Phanerochaetechrysosporium glucosidase, Uniparc reference UPI00006E0CC7, Uniprot reference Q25BW5B.

[0907] Seq ID No. 760: Codon-optimized DNA encoding 6xHis rye (Secale cereale) glucosidase, Uniparc reference UPI00000A7EFC, Uniprot reference Q9FYS3.

[0908] Seq ID No. 761: Codon-optimized DNA encoding 6xHis Anoxybacillus gonensis glucosidase, Uniparc reference UPI0002C0273E, Uniprot reference M5QUM2.

[0909] Seq ID No. 762: Codon-optimized DNA encoding 6xHis Thermotoga maritima glucosidase, Uniparc reference UPI0000126906, Uniprot reference Q08638A.

[0910] Seq ID No. 763: Codon-optimized DNA encoding 6xHis Rhodospirillum rubrum glucosidase, Uniparc reference UPI00003C2ACC, Uniprot reference Q2RP51.

[0911] Seq ID No. 764: Codon-optimized DNA encoding 6xHis Thermotoganea politana glucosidase, Uniparc reference UPI00000B9013, Uniprot reference Q60038.

[0912] Seq ID No. 765: Codon-optimized DNA encoding 6xHis Candidatus microthrix glucosidase, Uniparc reference UPI00032F466E, Uniprot reference R4Z6M6.

[0913] Seq ID No. 766: Codon-optimized DNA encoding 6xHis Ruminiclostridium cellobioparum glucosidase, Uniparc reference UPI00032874EC, Uniprot reference SOFPI8.

[0914] Seq ID No. 767: Codon-optimized DNA encoding 6xHis Acholeplasma brassicae glucosidase, Uniparc reference UPI0003B0490C, Uniprot reference U4KMV4.

[0915] Seq ID No. 768: Codon-optimized DNA encoding 6xHis Klebsiella pneumoniae glucosidase, Uniparc reference UPI0003DB6EFD, Uniprot reference W1BBP5.

[0916] Seq ID No. 769: Codon-optimized DNA encoding 6xHis Enterobacter sp. glucosidase, Uniparc reference UPI0003ED0A8E, Uniprot reference W7P6Y1.

[0917] Seq ID No. 770: Codon-optimized DNA encoding 6xHis Rhizoctonia solani glucosidase, Uniparc reference UPI00045BB507, Uniprot reference X8JI88.

[0918] Seq ID No. 771: Codon-optimized DNA encoding 6xHis Homo sapiens glucosidase, Uniparc reference UPI0000072C73, Uniprot reference Q9H227.

[0919] Seq ID No. 772: Codon-optimized DNA encoding 6xHis uncultured bacterial glucosidase, Uniparc reference UPI0000DD5304, Uniprot reference Q0GMU3.

[0920] Seq ID No. 773: Codon-optimized DNA encoding 6xHis Thermoanaerobacter thermohydrosulfuricus glucosidase, Uniparc reference UPI0002CA9E4F, Uniprot reference M8CQD9.

[0921] Seq ID No. 774: Codon-optimized DNA encoding 6xHis Stigmatella aurantiaca glucosidase, Uniparc reference UPI0000E297D8, Uniprot reference Q091M8.

[0922] Seq ID No. 775: Codon-optimized DNA encoding 6xHis Burkholderia thailandensis glucosidase, Uniparc reference UPI00006676B1, Uniprot reference Q2T7C9.

[0923] Seq ID No. 776: Codon-optimized DNA encoding 6xHis Yersinia pseudotuberculosis glucosidase, Uniparc reference UPI0000426E8F, Uniprot reference Q665S2.

[0924] Seq ID No. 777: Codon-optimized DNA encoding 6xHis Togninia minima glucosidase, Uniparc reference UPI00032BF71F, Uniprot reference R8BQM8.

[0925] Seq ID No. 778: Codon-optimized DNA encoding 6xHis Ophiostoma piceae glucosidase, Uniparc reference UPI00035213C2, Uniprot reference S3BVJ8.

[0926] Seq ID No. 779: Codon-optimized DNA encoding 6xHis Pyronemaomphalodes glucosidase, Uniparc reference UPI0003B117B7, Uniprot reference U4LIL3.

[0927] Seq ID No. 780: Codon-optimized DNA encoding 6xHis Ogataea parapolymorpha glucosidase, Uniparc reference UPI0001F76D1F, Uniprot reference W1QIW0.

[0928] Seq ID No. 781: Codon-optimized DNA encoding 6xHis Hymenobacter wuensis glucosidase, Uniparc reference UPI0003F2059F, Uniprot reference W8F6U0.

[0929] Seq ID No. 782: Codon-optimized DNA encoding 6xHis Flavobacterium johnsoniae glucosidase, Uniparc reference UPI00006E8E1B, Uniprot reference A5FEF5.

[0930] Seq ID No. 783: Codon-optimized DNA encoding 6xHis Paenibacillus polymyxa glucosidase, Uniparc reference UPI000012690B, Uniprot reference P22505.

[0931] Seq ID No. 784: Codon-optimized DNA encoding 6xHis rice (Oryza sativa) glucosidase, Uniparc reference UPI000009D014, Uniprot reference Q8L7J2.

[0932] Seq ID No. 785: Codon-optimized DNA encoding 6xHis rice (Oryza sativa) glucosidase, Uniparc reference UPI0000E580F2, Uniprot reference B8AVF0.

[0933] Seq ID No. 786: Codon-optimized DNA encoding 6xHis Nannochloris glucosidase, Uniparc reference UPI000EA7F16F, Uniprot reference A0A452CSM4.

[0934] Seq ID No. 787: Codon-optimized DNA encoding 6xHis Halothermothrixorenii glucosidase, Uniparc reference UPI00006AE508, Uniprot reference B8CYA8.

[0935] Seq ID No. 788: Codon-optimized DNA encoding 6xHis Neurospora crassa glucosidase, Uniparc reference UPI000018B2B4, Uniprot reference Q7RWP2.

[0936] Seq ID No. 789: Codon-optimized DNA encoding 6xHis Micrococcus antarcticus glucosidase, Uniparc reference UPI000192BB5F, Uniprot reference B9V8P5.

[0937] Seq ID No. 790: Codon-optimized DNA encoding 6xHis Exiguobacterium antarcticum glucosidase, Uniparc reference UPI000285E79E, Uniprot reference K0A8J9.

[0938] Seq ID No. 791: Codon-optimized DNA encoding 6xHis Thermus thermophilus glucosidase, Uniparc reference UPI00000BEB61, Uniprot reference Q9RA61.

[0939] Seq ID No. 792: Codon-optimized DNA encoding 6xHis Trichoderma harzianum glucosidase, Uniparc reference UPI00078BF747, Uniprot reference A0A2T4AR08.

[0940] Seq ID No. 793: Codon-optimized DNA encoding 6xHis Hypocrea jecorina glucosidase, Uniparc reference UPI000006AA61, Uniprot reference Q12715.

[0941] Seq ID No. 794: Codon-optimized DNA encoding 6xHis Streptomyces sp. glucosidase, Uniparc reference UPI00000B411B, Uniprot reference Q59976.

[0942] Seq ID No. 795: Codon-optimized DNA encoding 6xHis Streptococcus pyogenes glucosidase, Uniparc reference UPI00000C7E56, Uniprot reference Q99YP9.

[0943] Seq ID No. 796: Codon-optimized DNA encoding 6xHis Trifolium repens glucosidase, Uniparc reference UPI000012691B, Uniprot reference P26205.

[0944] Seq ID No. 797: Codon-optimized DNA encoding 6xHis Talaromyces emersonii glucosidase, Uniparc reference UPI000006C8FF, Uniprot reference Q8TGI8.

[0945] Seq ID No. 798: Codon-optimized DNA encoding 6xHis Hungateiclostridium thermocellum glucosidase, Uniparc reference UPI0000126903, Uniprot reference P26208.

[0946] Seq ID No. 799: Codon-optimized DNA encoding 6xHis Lactobacillus plantarum glucosidase, Uniparc reference UPI000219FE3E, Uniprot reference F9ULH8.

[0947] Seq ID No. 800: Codon-optimized DNA encoding 6xHis Agrobacterium tumefaciens glucosidase, Uniparc reference UPI0003F2033A, Uniprot reference A0A2I4PGZ0.

[0948] Seq ID No. 801: Amino acid sequence of Bifidobacterium actinocoloniiforme glucosidase, Uniparc reference UPI000503B26C, Uniprot reference A0A086YYS8.

[0949] Seq ID No. 802: Amino acid sequence of Bifidobacterium psychraerophilum glucosidase, Uniparc reference UPI000502B461, Uniprot reference A0A087CD28.

[0950] Seq ID No. 803: Amino acid sequence of: Penicillium italicum glucosidase, Uniparc reference UPI00052B8681, Uniprot reference A0A0A2K704.

[0951] Seq ID No. 804: Amino acid sequence of the following: Microbacterium trichothecenolyticum glucosidase, Uniparc reference UPI0005ED0AE6, Uniprot reference A0A0M2HDB3.

[0952] Seq ID No. 805: Amino acid sequence of uncultured Clostridium sp. glucosidase, Uniparc reference UPI0008206F38, Uniprot reference A0A1C6I6A3.

[0953] Seq ID No. 806: Amino acid sequence of the following: Bifiguratus adelaidae glucosidase, Uniparc reference UPI000BC57319, Uniprot reference A0A261Y7Q8.

[0954] Seq ID No. 807: Amino acid sequence of the following: Paenibacillus thiaminolyticus glucosidase, Uniparc reference UPI000B3B73CD, Uniprot reference A0A378ZIK3.

[0955] Seq ID No. 808: Amino acid sequence of the following: Microbacterium lemovicicum glucosidase, Uniparc reference UPI000F8FB9BA, Uniprot reference A0A3S9WE68.

[0956] Seq ID No. 809: Amino acid sequence of the following: Rhodococcus erythropolis glucosidase, Uniparc reference UPI00019923DC, Uniprot reference C1A1N4.

[0957] Seq ID No. 810: Amino acid sequence of the following: Cutibacterium avidum glucosidase, Uniparc reference UPI00022C19B5, Uniprot reference G4CZU8.

[0958] Seq ID No. 811: Amino acid sequence of the following: Clavibacter michiganensis glucosidase, Uniparc reference UPI0002C5A938, Uniprot reference M5BD10.

[0959] Seq ID No. 812: Amino acid sequence of Microbacterium sp. glucosidase, Uniparc reference UPI0003DE3509, Uniprot reference W0ZC23.

[0960] Seq ID No. 813: Amino acid sequence of Bifidobacterium actinocoloniiforme glucosidase, Uniparc reference UPI000507F38A, Uniprot reference A0A086YZI4.

[0961] Seq ID No. 814: Amino acid sequence of the following: Bifidobacterium reuteri glucosidase, Uniparc reference UPI000506119A, Uniprot reference A0A087CR26.

[0962] Seq ID No. 815: Amino acid sequence of the following: Propionibacterium freudenreichii glucosidase, Uniparc reference UPI0005A5CAC2, Uniprot reference A0A0A8RX48.

[0963] Seq ID No. 816: Amino acid sequence of the following: Microbacterium hydrocarbonoxydans glucosidase, Uniparc reference UPI0005EC18F9, Uniprot reference A0A0M2HRU0.

[0964] Seq ID No. 817: Amino acid sequence of: Pseudonocardia sp. glucosidase, Uniparc reference UPI00094B6D3B, Uniprot reference A0A1Q8KSV8.

[0965] Seq ID No. 818: Amino acid sequence of the following: Tuber aestivum glucosidase, Uniparc reference UPI000BC13DF0, Uniprot reference A0A292PKV5.

[0966] Seq ID No. 819: Amino acid sequence of the following: Propionibacterium australiense glucosidase, Uniparc reference UPI000E5B4EA5, Uniprot reference A0A383S7A9.

[0967] Seq ID No. 820: Amino acid sequence of the following: Fusarium sp. glucosidase, Uniparc reference UPI001004B2C9, Uniprot reference A0A428T6E2.

[0968] Seq ID No. 821: Amino acid sequence of the following: Nectria haematococca glucosidase, Uniparc reference UPI0001B69B5C, Uniprot reference C7ZBV0.

[0969] Seq ID No. 822: Amino acid sequence of Actinoplanes sp. glucosidase, Uniparc reference UPI00023EBB15, Uniprot reference G8S3E7.

[0970] Seq ID No. 823: Amino acid sequence of Streptomyces fulvissimus glucosidase, Uniparc reference UPI0003289BC6, Uniprot reference NOCMW2.

[0971] Seq ID No. 824: Amino acid sequence of the following: Pestalotiopsis fici glucosidase, Uniparc reference UPI0003E02BF1, Uniprot reference W3WXF1.

[0972] Seq ID No. 825: Amino acid sequence of the following: Bifidobacterium bohemicum glucosidase, Uniparc reference UPI0005025F2E, Uniprot reference A0A086ZGP0.

[0973] Seq ID No. 826: Amino acid sequence of the following: Biffdobacterium saeculare glucosidase, Uniparc reference UPI0005084E52, Uniprot reference A0A087D0Q2.

[0974] Seq ID No. 827: Amino acid sequence of: Bionectria ochroleuca glucosidase, Uniparc reference UPI00059674D6, Uniprot reference A0A0B7JW24.

[0975] Seq ID No. 828: Amino acid sequence of Bifidobacterium pseudocatenulatum glucosidase, Uniparc reference UPI0006C6D6F9, Uniprot reference A0A174AU04.

[0976] Seq ID No. 829: Amino acid sequence of Pseudonocardia sp. glucosidase, Uniparc reference UPI00094ABAB3, Uniprot reference A0A1Q8LPB4.

[0977] Seq ID No. 830: Amino acid sequence of the following: bacterial glucosidase, Uniparc reference UPI000CB49A0B, Uniprot reference A0A2H5Z8Q4.

[0978] Seq ID No. 831: Amino acid sequence of the following: Coleophoma crateriformis glucosidase, Uniparc reference UPI000E38A995, Uniprot reference A0A3D8Q771.

[0979] Seq ID No. 832: Amino acid sequence of the following: Arthrobotrys oligospora glucosidase, Uniparc reference UPI001102DFA3, Uniprot reference A0A4Z0Y5Y8.

[0980] Seq ID No. 833: Amino acid sequence of the following: Pyrenophora teres glucosidase, Uniparc reference UPI0001ECDCBD, Uniprot reference E3RFS2.

[0981] Seq ID No. 834: Amino acid sequence of Gordonia polyisoprenivorans glucosidase, Uniparc reference UPI00024F2A26, Uniprot reference H6MTQ7.

[0982] Seq ID No. 835: Amino acid sequence of the following: Stigmatella aurantiaca glucosidase, Uniparc reference UPI0000E28E5D, Uniprot reference Q08S21.

[0983] Seq ID No. 836: Amino acid sequence of the following: Pestalotiopsis fici glucosidase, Uniparc reference UPI0003E03A52, Uniprot reference W3WZ03.

[0984] Seq ID No. 837: Amino acid sequence of Bifidobacterium magnum glucosidase, Uniparc reference UPI0003B7B6EE, Uniprot reference A0A087BEN9.

[0985] Seq ID No. 838: Amino acid sequence of Bifidobacterium stellenboschense glucosidase, Uniparc reference UPI000503F283, Uniprot reference A0A087DFL8.

[0986] Seq ID No. 839: Amino acid sequence of: Bionectria ochroleuca glucosidase, Uniparc reference UPI00059673EE, Uniprot reference A0A0B7K316.

[0987] Seq ID No. 840: Amino acid sequence of the following: Hungatella hathewayi glucosidase, Uniparc reference UPI0006C069F3, Uniprot reference A0A174LVE3.

[0988] Seq ID No. 841: Amino acid sequence of the following: Mycetocolare bloconi glucosidase, Uniparc reference UPI00097EB800, Uniprot reference A0A1R4J2F9.

[0989] Seq ID No. 842: Amino acid sequence of the following: Nonomuraea sp. glucosidase, Uniparc reference UPI0009ABD7B3, Uniprot reference A0A2P9IX34.

[0990] Seq ID No. 843: Amino acid sequence of the following: Coleophoma crateriformis glucosidase, Uniparc reference UPI000E391DF6, Uniprot reference A0A3D8T9C2.

[0991] Seq ID No. 844: Amino acid sequence of the following: Paenarthrobacter aurescens glucosidase, Uniparc reference UPI0000EC83AE, Uniprot reference A1R2K1.

[0992] Seq ID No. 845: Amino acid sequence of the following: Kitasatospora setae glucosidase, Uniparc reference UPI0001F21F08, Uniprot reference E4N4F6.

[0993] Seq ID No. 846: Amino acid sequence of Nocardiopsis alba glucosidase, Uniparc reference UPI00027E251E, Uniprot reference J7L3Z8.

[0994] Seq ID No. 847: Amino acid sequence of the following: Rhodococcus jostii glucosidase, Uniparc reference UPI0000DBA338, Uniprot reference Q0SCI4.

[0995] Seq ID No. 848: Amino acid sequence of the following: (uncultured microorganism) glucosidase, Uniparc reference UPI0003EC942D, Uniprot reference W5X324.

[0996] Seq ID No. 849: Amino acid sequence of the following: Bifidobacterium merycicum glucosidase, Uniparc reference UPI0005084B63, Uniprot reference A0A087BJ88.

[0997] Seq ID No. 850: Amino acid sequence of the following: Bifidobacterium scardovii glucosidase, Uniparc reference UPI0005018B5B, Uniprot reference A0A087DGT3.

[0998] Seq ID No. 851: Amino acid sequence of the following: Fusarium oxysporum glucosidase, Uniparc reference UPI00021EC81E, Uniprot reference A0A0C4DJL4.

[0999] Seq ID No. 852: Amino acid sequence of the following: Paraphaeosphaeria sporulosa glucosidase, Uniparc reference UPI0007CE199C, Uniprot reference A0A177BXH1.

[1000] Seq ID No. 853: Amino acid sequence of Clostridium oryzae glucosidase, Uniparc reference UPI0009A50F8D, Uniprot reference A0A1V4IY77.

[1001] Seq ID No. 854: Amino acid sequence of the following: Corynespora cassiicola glucosidase, Uniparc reference UPI000D22F5EB, Uniprot reference A0A2T2N4T6.

[1002] Seq ID No. 855: Amino acid sequence of the following: Choiromyces venosus glucosidase, Uniparc reference UPI000F7332F8, Uniprot reference A0A3N4J9R6.

[1003] Seq ID No. 856: Amino acid sequence of the following: Saccharopolyspora erythraea glucosidase, Uniparc reference UPI0000F550EC, Uniprot reference A4F982.

[1004] Seq ID No. 857: Amino acid sequence of the following: Streptomyces venezuelae glucosidase, Uniparc reference UPI000204906E, Uniprot reference F2R0Y2.

[1005] Seq ID No. 858: Amino acid sequence of: Arthrobacter sp. glucosidase, Uniparc reference UPI00027DFDB0, Uniprot reference J7LVB1.

[1006] Seq ID No. 859: Amino acid sequence of the following: Thermobrachium celere glucosidase, Uniparc reference UPI00033420CB, Uniprot reference R7RMQ6.

[1007] Seq ID No. 860: Amino acid sequence of: Drechslerella stenobrocha glucosidase, Uniparc reference UPI0003EA65B9, Uniprot reference W7HT93.

[1008] Seq ID No. 861: Amino acid sequence of the following: Bifidobacterium minimum glucosidase, Uniparc reference UPI0003B38542, Uniprot reference A0A087BMR5.

[1009] Seq ID No. 862: Amino acid sequence of: Bifidobacterium thermocidophilum glucosidase, Uniparc reference UPI000409825E, Uniprot reference A0A087E3K8.

[1010] Seq ID No. 863: Amino acid sequence of the following: Verruconis gallopava glucosidase, Uniparc reference UPI0005C0229F, Uniprot reference A0A0D1X9G0.

[1011] Seq ID No. 864: Amino acid sequence of the following: Stagonospora sp. glucosidase, Uniparc reference UPI0007CECAD5, Uniprot reference A0A178AER7.

[1012] Seq ID No. 865: Amino acid sequence of the following: Firmicutes bacterium glucosidase, Uniparc reference UPI0009D58C49, Uniprot reference A0A1V6ALF9.

[1013] Seq ID No. 866: Amino acid sequence of the following: Tuber borchii glucosidase, Uniparc reference UPI000D50EED1, Uniprot reference A0A2T6ZNU7.

[1014] Seq ID No. 867: Amino acid sequence of the following: Morchella conica glucosidase, Uniparc reference UPI000F728167, Uniprot reference A0A3N4KUR3.

[1015] Seq ID No. 868: Amino acid sequence of the following: Dictyoglomus thermophilum glucosidase, Uniparc reference UPI00018152EB, Uniprot reference B5YC96.

[1016] Seq ID No. 869: Amino acid sequence of the following: Microlunatus phosphovorus glucosidase, Uniparc reference UPI000210C01C, Uniprot reference F5XL24.

[1017] Seq ID No. 870: Amino acid sequence of the following: Bifidobacterium asteroides glucosidase, Uniparc reference UPI00028BB1B6, Uniprot reference K4IPD2.

[1018] Seq ID No. 871: Amino acid sequence of: Dactylellina haptotyla glucosidase, Uniparc reference UPI00035AE576, Uniprot reference S8BQ60.

[1019] Seq ID No. 872: Amino acid sequence of the following: Fusarium oxysporum glucosidase, Uniparc reference UPI0003F2D7DD, Uniprot reference W9JF54.

[1020] Seq ID No. 873: Amino acid sequence of the following: Bifidobacterium longum glucosidase, Uniparc reference UPI0005067FF9, Uniprot reference A0A087BRY2.

[1021] Seq ID No. 874: Amino acid sequence of: Bifidobacterium tsurumiense glucosidase, Uniparc reference UPI0004105550, Uniprot reference A0A087EK01.

[1022] Seq ID No. 875: Amino acid sequence of the following: Exophiala spinifera glucosidase, Uniparc reference UPI0005BF9DF9, Uniprot reference A0A0D1YG50.

[1023] Seq ID No. 876: Amino acid sequence of the following: Pyrenochaeta sp. glucosidase, Uniparc reference UPI0007CE7DBA, Uniprot reference A0A178E5I1.

[1024] Seq ID No. 877: Amino acid sequence of the following: Firmicutes bacterium glucosidase, Uniparc reference UPI0009CDA3F1, Uniprot reference A0A1V6FLK2.

[1025] Seq ID No. 878: Amino acid sequence of the following: Cadophora sp. glucosidase, Uniparc reference UPI000D5A9E68, Uniprot reference A0A2V1B599.

[1026] Seq ID No. 879: Amino acid sequence of the following: Morchella conica glucosidase, Uniparc reference UPI000F7330CD, Uniprot reference A0A3N4L4M3.

[1027] Seq ID No. 880: Amino acid sequence of the following: Bifidobacterium animalis glucosidase, Uniparc reference UPI000189C68F, Uniprot reference B8DV42.

[1028] Seq ID No. 881: Amino acid sequence of the following: Treponema azotonutricium glucosidase, Uniparc reference UPI00020ED2C9, Uniprot reference F5YGD5.

[1029] Seq ID No. 882: Amino acid sequence of the following: Acidipropionibacterium acidipropionici glucosidase, Uniparc reference UPI0002988429, Uniprot reference K7S0E5.

[1030] Seq ID No. 883: Amino acid sequence of: Salinispira pacifica glucosidase, Uniparc reference UPI0003D93613, Uniprot reference V5WNB6.

[1031] Seq ID No. 884: Amino acid sequence of the following: Bifidobacterium mongoliense glucosidase, Uniparc reference UPI000503588E, Uniprot reference A0A087C760.

[1032] Seq ID No. 885: Amino acid sequence of the following: Bifidobacterium indicum glucosidase, Uniparc reference UPI000499F5D1, Uniprot reference A0A087VUW4.

[1033] Seq ID No. 886: Amino acid sequence of Brachyspira suanatina glucosidase, Uniparc reference UPI000659257F, Uniprot reference A0A0G4K5C2.

[1034] Seq ID No. 887: Amino acid sequence of: Phialocephala scopiformis glucosidase, Uniparc reference UPI0007F2FD44, Uniprot reference A0A194X8Q9.

[1035] Seq ID No. 888: Amino acid sequence of the following: Uncultured Microbacterium sp. (glucosidase), Uniparc reference UPI000A2BB4A5, Uniprot reference A0A1Y5P895.

[1036] Seq ID No. 889: Amino acid sequence of the following: Periconia macrospinosa glucosidase, Uniparc reference UPI000D5BC9BD, Uniprot reference A0A2V1E673.

[1037] Seq ID No. 890: Amino acid sequence of: Arthrobacter ulceris almonis glucosidase, Uniparc reference UPI000F3DC30B, Uniprot reference A0A3P5WTE2.

[1038] Seq ID No. 891: Amino acid sequence of the following: Pseudarthrobacter chlorophenolicus glucosidase, Uniparc reference UPI0001664880, Uniprot reference B8H9A2.

[1039] Seq ID No. 892: Amino acid sequence of the following: Haloplasma contractile glucosidase, Uniparc reference UPI0002120C63, Uniprot reference F7PUF3.

[1040] Seq ID No. 893: Amino acid sequence of the following: Cochliobolusheterostrophus glucosidase, Uniparc reference UPI0002B73341, Uniprot reference M2ULB2.

[1041] Seq ID No. 894: Amino acid sequence of Microbacterium sp. glucosidase, Uniparc reference UPI0003DE58EA, Uniprot reference W0Z818.

[1042] Seq ID No. 895: Codon-optimized DNA encoding 6xHis Bifidobacterium actinocoloniiforme glucosidase, Uniparc reference UPI000503B26C, Uniprot reference A0A086YYS8.

[1043] Seq ID No. 896: Codon-optimized DNA encoding 6xHis Bifidobacterium psychraerophilum glucosidase, Uniparc reference UPI000502B461, Uniprot reference A0A087CD28.

[1044] Seq ID No. 897: Codon-optimized DNA encoding 6xHis Penicillium italicum glucosidase, Uniparc reference UPI00052B8681, Uniprot reference A0A0A2K704.

[1045] Seq ID No. 898: Codon-optimized DNA encoding 6xHis Microbacterium trichothecenolyticum glucosidase, Uniparc reference UPI0005ED0AE6, Uniprot reference A0A0M2HDB3.

[1046] Seq ID No. 899: Codon-optimized DNA encoding 6xHis uncultured Clostridium sp. glucosidase, Uniparc reference UPI0008206F38, Uniprot reference A0A1C6I6A3.

[1047] Seq ID No. 900: Codon-optimized DNA encoding 6xHis Bifiguratus adelaidae glucosidase, Uniparc reference UPI000BC57319, Uniprot reference A0A261Y7Q8.

[1048] Seq ID No. 901: Codon-optimized DNA encoding 6xHis Paenibacillus thiaminolyticus glucosidase, Uniparc reference UPI000B3B73CD, Uniprot reference A0A378ZIK3.

[1049] Seq ID No. 902: Codon-optimized DNA encoding 6xHis Microbacterium lemovicicum glucosidase, Uniparc reference UPI000F8FB9BA, Uniprot reference A0A3S9WE68.

[1050] Seq ID No. 903: Codon-optimized DNA encoding 6xHis Rhodococcus erythropolis glucosidase, Uniparc reference UPI00019923DC, Uniprot reference C1A1N4.

[1051] Seq ID No. 904: Codon-optimized DNA encoding 6xHis Cutibacterium avidum glucosidase, Uniparc reference UPI00022C19B5, Uniprot reference G4CZU8.

[1052] Seq ID No. 905: Codon-optimized DNA encoding 6xHis Clavibacter michiganensis glucosidase, Uniparc reference UPI0002C5A938, Uniprot reference M5BD10.

[1053] Seq ID No. 906: Codon-optimized DNA encoding 6xHis Microbacterium sp. glucosidase, Uniparc reference UPI0003DE3509, Uniprot reference W0ZC23.

[1054] Seq ID No. 907: Codon-optimized DNA encoding 6xHis Bifidobacterium actinocoloniiforme glucosidase, Uniparc reference UPI000507F38A, Uniprot reference A0A086YZL4.

[1055] Seq ID No. 908: Codon-optimized DNA encoding 6xHis Bifidobacterium reuteri glucosidase, Uniparc reference UPI000506119A, Uniprot reference A0A087CR26.

[1056] Seq ID No. 909: Codon-optimized DNA encoding 6xHis Propionibacterium freudenreichii glucosidase, Uniparc reference UPI0005A5CAC2, Uniprot reference A0A0A8RX48.

[1057] Seq ID No. 910: Codon-optimized DNA encoding 6xHis Microbacterium hydrocarbonoxydans glucosidase, Uniparc reference UPI0005EC18F9, Uniprot reference A0A0M2HRU0.

[1058] Seq ID No. 911: Codon-optimized DNA encoding 6xHis Pseudonocardia sp. glucosidase, Uniparc reference UPI00094B6D3B, Uniprot reference A0A1Q8KSV8.

[1059] Seq ID No. 912: Codon-optimized DNA encoding 6xHis Tuber aestivum glucosidase, Uniparc reference UPI000BC13DF0, Uniprot reference A0A292PKV5.

[1060] Seq ID No. 913: Codon-optimized DNA encoding 6xHis Propionibacterium australiense glucosidase, Uniparc reference UPI000E5B4EA5, Uniprot reference A0A383S7A9.

[1061] Seq ID No. 914: Codon-optimized DNA encoding 6xHis Fusarium sp. glucosidase, Uniparc reference UPI001004B2C9, Uniprot reference A0A428T6E2.

[1062] Seq ID No. 915: Codon-optimized DNA encoding 6xHis glucosidase from the flagellate Nectria haematococca, Uniparc reference UPI0001B69B5C, Uniprot reference C7ZBV0.

[1063] Seq ID No. 916: Codon-optimized DNA encoding 6xHis Actinoplanes sp. glucosidase, Uniparc reference UPI00023EBB15, Uniprot reference G8S3E7.

[1064] Seq ID No. 917: Codon-optimized DNA encoding 6xHis Streptomyces fulvissimus glucosidase, Uniparc reference UPI0003289BC6, Uniprot reference NOCMW2.

[1065] Seq ID No. 918: Codon-optimized DNA encoding 6xHis Pestalotiopsis fici glucosidase, Uniparc reference UPI0003E02BF1, Uniprot reference W3WXF1.

[1066] Seq ID No. 919: Codon-optimized DNA encoding 6xHis Bifidobacterium bohemicum glucosidase, Uniparc reference UPI0005025F2E, Uniprot reference A0A086ZGP0.

[1067] Seq ID No. 920: Codon-optimized DNA encoding 6xHis Bifidobacterium saeculare glucosidase, Uniparc reference UPI0005084E52, Uniprot reference A0A087D0Q2. Seq ID No. 921: Codon-optimized DNA encoding 6xHis Bionectria ochroleuca glucosidase, Uniparc reference UPI00059674D6, Uniprot reference A0A0B7JW24.

[1068] Seq ID No. 922: Codon-optimized DNA encoding 6xHis Bifidobacterium pseudocatenulatum glucosidase, Uniparc reference UPI0006C6D6F9, Uniprot reference A0A174AU04.

[1069] Seq ID No. 923: Codon-optimized DNA encoding 6xHis Pseudocardia sp. glucosidase, Uniparc reference UPI00094ABAB3, Uniprot reference A0A1Q8LPB4.

[1070] Seq ID No. 924: Codon-optimized DNA encoding 6xHis bacterial glucosidase, Uniparc reference UPI000CB49A0B, Uniprot reference A0A2H5Z8Q4.

[1071] Seq ID No. 925: Codon-optimized DNA encoding 6xHis Coleophoma crateriformis glucosidase, Uniparc reference UPI000E38A995, Uniprot reference A0A3D8Q771.

[1072] Seq ID No. 926: Codon-optimized DNA encoding 6xHis Arthrobotrysoligospora glucosidase, Uniparc reference UPI001102DFA3, Uniprot reference A0A4Z0Y5Y8.

[1073] Seq ID No. 927: Codon-optimized DNA encoding 6xHis Pyrenophorateres glucosidase, Uniparc reference UPI0001ECDCBD, Uniprot reference E3RFS2.

[1074] Seq ID No. 928: Codon-optimized DNA encoding 6xHis Gordonia polyisoprenivorans glucosidase, Uniparc reference UPI00024F2A26, Uniprot reference H6MTQ7.

[1075] Seq ID No. 929: Codon-optimized DNA encoding 6xHis Stigmatella aurantiaca glucosidase, Uniparc reference UPI0000E28E5D, Uniprot reference Q08S21.

[1076] Seq ID No. 930: Codon-optimized DNA encoding 6xHis Pestalotiopsis fici glucosidase, Uniparc reference UPI0003E03A52, Uniprot reference W3WZ03.

[1077] Seq ID No. 931: Codon-optimized DNA encoding 6xHis Bifidobacterium maxum glucosidase, Uniparc reference UPI0003B7B6EE, Uniprot reference A0A087BEN9.

[1078] SeqID No. 932: Codon-optimized DNA encoding 6xHis Bifidobacterium stellenboschense glucosidase, Uniparc reference UPI000503F283, Uniprot reference A0A087DFL8.

[1079] Seq ID No. 933: Codon-optimized DNA encoding 6xHis Bionectria ochroleuca glucosidase, Uniparc reference UPI00059673EE, Uniprot reference A0A0B7K316.

[1080] Seq ID No. 934: Codon-optimized DNA encoding 6xHis Hungatella hathewayi glucosidase, Uniparc reference UPI0006C069F3, Uniprot reference A0A174LVE3.

[1081] Seq ID No. 935: Codon-optimized DNA encoding 6xHis Mycetocolareblochoni glucosidase, Uniparc reference UPI00097EB800, Uniprot reference A0A1R4J2F9.

[1082] Seq ID No. 936: Codon-optimized DNA encoding 6xHis Nonomurae asp. glucosidase, Uniparc reference UPI0009ABD7B3, Uniprot reference A0A2P9IX34.

[1083] Seq ID No. 937: Codon-optimized DNA encoding 6xHis Coleophoma crateriformis glucosidase, Uniparc reference UPI000E391DF6, Uniprot reference A0A3D8T9C2.

[1084] Seq ID No. 938: Codon-optimized DNA encoding 6xHis Paenarthrobacter aurescens glucosidase, Uniparc reference UPI0000EC83AE, Uniprot reference A1R2K1.

[1085] Seq ID No. 939: Codon-optimized DNA encoding 6xHis Kitasatosporasetae glucosidase, Uniparc reference UPI0001F21F08, Uniprot reference E4N4F6.

[1086] Seq ID No. 940: Codon-optimized DNA encoding 6xHis Nocardiopsis alba glucosidase, Uniparc reference UPI00027E251E, Uniprot reference J7L3Z8.

[1087] Seq ID No. 941: Codon-optimized DNA encoding 6xHis Rhodococcus jostii glucosidase, Uniparc reference UPI0000DBA338, Uniprot reference Q0SCI4.

[1088] Seq ID No. 942: Codon-optimized DNA encoding 6xHis (uncultured microorganism) glucosidase, Uniparc reference UPI0003EC942D, Uniprot reference W5X324.

[1089] Seq ID No. 943: Codon-optimized DNA encoding 6xHis Bifidobacterium merycicum glucosidase, Uniparc reference UPI0005084B63, Uniprot reference A0A087BJ88.

[1090] Seq ID No. 944: Codon-optimized DNA encoding 6xHis Bifidobacterium scardovii glucosidase, Uniparc reference UPI0005018B5B, Uniprot reference A0A087DGT3.

[1091] Seq ID No. 945: Codon-optimized DNA encoding 6xHis Fusarium oxysporum glucosidase, Uniparc reference UPI00021EC81E, Uniprot reference A0A0C4DJL4.

[1092] Seq ID No. 946: Codon-optimized DNA encoding 6xHis Paraphaeosphaeria sporulosa glucosidase, Uniparc reference UPI0007CE199C, Uniprot reference A0A177BXH1.

[1093] Seq ID No. 947: Codon-optimized DNA encoding 6xHis Clostridium oryzae glucosidase, Uniparc reference UPI0009A50F8D, Uniprot reference A0A1V4IY77.

[1094] Seq ID No. 948: Codon-optimized DNA encoding 6xHis Corynespora cassiicola glucosidase, Uniparc reference UPI000D22F5EB, Uniprot reference A0A2T2N4T6.

[1095] Seq ID No. 949: Codon-optimized DNA encoding 6xHis Choiromyces venosus glucosidase, Uniparc reference UPI000F7332F8, Uniprot reference A0A3N4J9R6.

[1096] Seq ID No. 950: Codon-optimized DNA encoding 6xHis Saccharopolyspora erythraea glucosidase, Uniparc reference UPI0000F550EC, Uniprot reference A4F982.

[1097] Seq ID No. 951: Codon-optimized DNA encoding 6x His Streptomyces venezuelae glucosidase, Uniparc reference UPI000204906E, Uniprot reference F2R0Y2.

[1098] Seq ID No. 952: Codon-optimized DNA encoding 6xHis Arthrobacter sp. glucosidase, Uniparc reference UPI00027DFDB0, Uniprot reference J7LVB1.

[1099] Seq ID No. 953: Codon-optimized DNA encoding 6xHis Thermobrachium celere glucosidase, Uniparc reference UPI00033420CB, Uniprot reference R7RMQ6.

[1100] Seq ID No. 954: Codon-optimized DNA encoding 6xHis Drechslerella stenobrocha glucosidase, Uniparc reference UPI0003EA65B9, Uniprot reference W7HT93.

[1101] Seq ID No. 955: Codon-optimized DNA encoding 6xHis Bifidobacterium minimum glucosidase, Uniparc reference UPI0003B38542, Uniprot reference A0A087BMR5.

[1102] Seq ID No. 956: Codon-optimized DNA encoding 6xHis Bifidobacterium thermocidophilum glucosidase, Uniparc reference UPI000409825E, Uniprot reference A0A087E3K8.

[1103] Seq ID No. 957: Codon-optimized DNA encoding 6xHis Verruconis gallopava glucosidase, Uniparc reference UPI0005C0229F, Uniprot reference A0A0D1X9G0.

[1104] Seq ID No. 958: Codon-optimized DNA encoding 6xHis Stagonospora sp. glucosidase, Uniparc reference UPI0007CECAD5, Uniprot reference A0A178AER7.

[1105] Seq ID No. 959: Codon-optimized DNA encoding 6xHis Firmicutes bacterium glucosidase, Uniparc reference UPI0009D58C49, Uniprot reference A0A1V6ALF9.

[1106] Seq ID No. 960: Codon-optimized DNA encoding 6xHis Tuberborchii glucosidase, Uniparc reference UPI000D50EED1, Uniprot reference A0A2T6ZNU7.

[1107] Seq ID No. 961: Codon-optimized DNA encoding 6xHis Morchella conica glucosidase, Uniparc reference UPI000F728167, Uniprot reference A0A3N4KUR3.

[1108] Seq ID No. 962: Codon-optimized DNA encoding 6xHis Dictyoglomus thermophilum glucosidase, Uniparc reference UPI00018152EB, Uniprot reference B5YC96.

[1109] Seq ID No. 963: Codon-optimized DNA encoding 6xHis Microlunatus phosphovorus glucosidase, Uniparc reference UPI000210C01C, Uniprot reference F5XL24.

[1110] Seq ID No. 964: Codon-optimized DNA encoding 6xHis Bifidobacterium asteroides glucosidase, Uniparc reference UPI00028BB1B6, Uniprot reference K4IPD2.

[1111] Seq ID No. 965: Codon-optimized DNA encoding 6xHis Dactylellina haptotyla glucosidase, Uniparc reference UPI00035AE576, Uniprot reference S8BQ60.

[1112] Seq ID No. 966: Codon-optimized DNA encoding 6xHis Fusarium oxysporum glucosidase, Uniparc reference UPI0003F2D7DD, Uniprot reference W9JF54.

[1113] Seq ID No. 967: Codon-optimized DNA encoding 6xHis Bifidobacterium longum glucosidase, Uniparc reference UPI0005067FF9, Uniprot reference A0A087BRY2.

[1114] Seq ID No. 968: Codon-optimized DNA encoding 6xHis Bifidobacterium tsurumiense glucosidase, Uniparc reference UPI0004105550, Uniprot reference A0A087EK01.

[1115] Seq ID No. 969: Codon-optimized DNA encoding 6xHis Exophialaspinifera glucosidase, Uniparc reference UPI0005BF9DF9, Uniprot reference A0A0D1YG50.

[1116] Seq ID No. 970: Codon-optimized DNA encoding 6xHis Pyrenochaetasp. glucosidase, Uniparc reference UPI0007CE7DBA, Uniprot reference A0A178E5I1.

[1117] Seq ID No. 971: Codon-optimized DNA encoding 6xHis Firmicutes bacterium glucosidase, Uniparc reference UPI0009CDA3F1, Uniprot reference A0A1V6FLK2.

[1118] Seq ID No. 972: Codon-optimized DNA encoding 6xHis Cadophora sp. glucosidase, Uniparc reference UPI000D5A9E68, Uniprot reference A0A2V1B599.

[1119] Seq ID No. 973: Codon-optimized DNA encoding 6xHis Morchella conica glucosidase, Uniparc reference UPI000F7330CD, Uniprot reference A0A3N4L4M3.

[1120] Seq ID No. 974: Codon-optimized DNA encoding 6xHis Bifidobacterium animalis glucosidase, Uniparc reference UPI000189C68F, Uniprot reference B8DV42.

[1121] Seq ID No. 975: Codon-optimized DNA encoding 6xHis Treponema azotonutricium glucosidase, Uniparc reference UPI00020ED2C9, Uniprot reference F5YGD5.

[1122] Seq ID No. 976: Codon-optimized DNA encoding 6xHis Acidipropionibacterium acidipropionici glucosidase, Uniparc reference UPI0002988429, Uniprot reference K7S0E5.

[1123] Seq ID No. 977: Codon-optimized DNA encoding 6xHis Salinispira pacifica glucosidase, Uniparc reference UPI0003D93613, Uniprot reference V5WNB6.

[1124] Seq ID No. 978: Codon-optimized DNA encoding 6xHis Bifidobacterium mongoliense glucosidase, Uniparc reference UPI000503588E, Uniprot reference A0A087C760.

[1125] Seq ID No. 979: Codon-optimized DNA encoding 6xHis Bifidobacterium indicum glucosidase, Uniparc reference UPI000499F5D1, Uniprot reference A0A087VUW4.

[1126] Seq ID No. 980: Codon-optimized DNA encoding 6xHis Brachyspira suanatina glucosidase, Uniparc reference UPI000659257F, Uniprot reference A0A0G4K5C2.

[1127] Seq ID No. 981: Codon-optimized DNA encoding 6xHis Phialocephala scopiformis glucosidase, Uniparc reference UPI0007F2FD44, Uniprot reference A0A194X8Q9.

[1128] Seq ID No. 982: Codon-optimized DNA encoding 6xHis (uncultured Microbacterium sp.) glucosidase, Uniparc reference UPI000A2BB4A5, Uniprot reference A0A1Y5P895.

[1129] Seq ID No. 983: Codon-optimized DNA encoding 6xHis Periconia macrospinosa glucosidase, Uniparc reference UPI000D5BC9BD, Uniprot reference A0A2V1E673.

[1130] Seq ID No. 984: Codon-optimized DNA encoding 6xHis Arthrobacter ulceris almonis glucosidase, Uniparc reference UPI000F3DC30B, Uniprot reference A0A3P5WTE2.

[1131] Seq ID No. 985: Codon-optimized DNA encoding 6xHis Pseudarthrobacter chlorophenolicus glucosidase, Uniparc reference UPI0001664880, Uniprot reference B8H9A2.

[1132] Seq ID No. 986: Codon-optimized DNA encoding 6xHis Haloplasma contractile glucosidase, Uniparc reference UPI0002120C63, Uniprot reference F7PUF3.

[1133] Seq ID No. 987: Codon-optimized DNA encoding 6xHis Cochliobolusheterostrophus glucosidase, Uniparc reference UPI0002B73341, Uniprot reference M2ULB2.

[1134] Seq ID No. 988: Codon-optimized DNA encoding 6xHis Microbacterium sp. glucosidase, Uniparc reference UPI0003DE58EA, Uniprot reference W0Z818.

[1135] Seq ID No. 989: Amino acid sequence of the following: Rhodothermus marinus rhamnosidase, Uniparc reference UPI0001A31108, Uniprot reference DOMFR0.

[1136] Seq ID No. 990: Amino acid sequence of the following: Streptomyces bingchenggensis rhamnosidase, Uniparc reference UPI0001D90BFE, Uniprot reference D7C463.

[1137] Seq ID No. 991: Amino acid sequence of Spirosoma linguale rhamnosidase, Uniparc reference UPI0001A3AEAC, Uniprot reference D2QL60.

[1138] Seq ID No. 992: Amino acid sequence of Roseburia intestinalis rhamnosidase, Uniparc reference UPI0001CD6D48, Uniprot reference D4L2K8.

[1139] Seq ID No. 993: Amino acid sequence of the following: Draconibacterium orientale rhamnosidase, Uniparc reference UPI000442EF22, Uniprot reference X5DG83.

[1140] Seq ID No. 994: Amino acid sequence of Catenulispora acidiphila rhamnosidase, Uniparc reference UPI00019E052B, Uniprot reference C7QC24.

[1141] Seq ID No. 995: Amino acid sequence of Bacteroides thetaiotaomicron rhamnosidase, Uniparc reference UPI000005ADE1, Uniprot reference Q8A916.

[1142] Seq ID No. 996: Amino acid sequence of: Opitutus terrae rhamnosidase, Uniparc reference UPI000172B2E3, Uniprot reference B1ZRE4.

[1143] Seq ID No. 997: Amino acid sequence of the following: Lachnoclostridium phytofermentans rhamnosidase, Uniparc reference UPI00015FE0BE, Uniprot reference A9KJP8.

[1144] Seq ID No. 998: Amino acid sequence of the following: Rhodanobacter denitrificans rhamnosidase, Uniparc reference UPI00022DA3ED, Uniprot reference M4NH01.

[1145] Seq ID No. 999: Amino acid sequence of the following: Prevotella ruminicola rhamnosidase, Uniparc reference UPI0001D07633, Uniprot reference D5ETD9.

[1146] Seq ID No. 1000: Amino acid sequence of: Aspergillus terreus rhamnosidase, Uniparc reference UPI000259E388, Uniprot reference I0AZ41.

[1147] Seq ID No. 1001: Amino acid sequence of Brachybacterium faecium rhamnosidase, Uniparc reference UPI0001A461B6, Uniprot reference C7MA58.

[1148] Seq ID No. 1002: Amino acid sequence of the following: Flavobacterium johnsoniae rhamnosidase, Uniparc reference UPI00006E4A0D, Uniprot reference A5FCH3.

[1149] Seq ID No. 1003: Amino acid sequence of the following: Rahnella aquatilis rhamnosidase, Uniparc reference UPI000245C507, Uniprot reference H2IYR2.

[1150] Seq ID No. 1004: Amino acid sequence of Bifidobacterium moukalabense rhamnosidase, Uniparc reference UPI0003E4E711, Uniprot reference W4N6H0.

[1151] Seq ID No. 1005: Amino acid sequence of Enterococcus casseliflavus rhamnosidase, Uniparc reference UPI000353BD9F, Uniprot reference S4BBS9.

[1152] Seq ID No. 1006: Amino acid sequence of Geobacillus sp. rhamnosidase, Uniparc reference UPI0001789C0D, Uniprot reference D3EED1.

[1153] Seq ID No. 1007: Amino acid sequence of the following: Rhamnosidase from Oligococcus marinus, Uniparc reference UPI000260A2FE, Uniprot reference I4EYD9.

[1154] Seq ID No. 1008: Amino acid sequence of the following: Pedobacter heparinus rhamnosidase, Uniparc reference UPI00019EF0EE, Uniprot reference C6XYM6.

[1155] Seq ID No. 1009: Amino acid sequence of: Dyadobacter fermentans rhamnosidase, Uniparc reference UPI00019B5915, Uniprot reference C6VZL3.

[1156] Seq ID No. 1010: Amino acid sequence of Paenibacillus mucilaginosus rhamnosidase, Uniparc reference UPI000212C156, Uniprot reference F8FQQ3.

[1157] Seq ID No. 1011: Amino acid sequence of Paenibacillus sp. rhamnosidase, Uniparc reference UPI0003E2544D, Uniprot reference W4D866.

[1158] Seq ID No. 1012: Amino acid sequence of Bacteroides thetaiotaomicron rhamnosidase, Uniparc reference UPI000005ADD2, Uniprot reference A0A0P0FM19.

[1159] Seq ID No. 1013: Amino acid sequence of Chloroflexus aurantiacus rhamnosidase, Uniparc reference UPI00005BA60B, Uniprot reference A9WDK5.

[1160] Seq ID No. 1014: Amino acid sequence of Thermoclostridium stercorarium rhamnosidase, Uniparc reference UPI00000B098C, Uniprot reference Q9S3L0.

[1161] Seq ID No. 1015: Amino acid sequence of Bifidobacterium moukalabense rhamnosidase, Uniparc reference UPI0003E5C314, Uniprot reference W4N6I2.

[1162] Seq ID No. 1016: Amino acid sequence of the following: Olsenella profusa rhamnosidase, Uniparc reference UPI0003AE032C, Uniprot reference U2USP4.

[1163] Seq ID No. 1017: Amino acid sequence of the following: Kribbella flavida rhamnosidase, Uniparc reference UPI00019BDB13, Uniprot reference D2PMT5.

[1164] Seq ID No. 1018: Amino acid sequence of Caulobacter vibrioides rhamnosidase, Uniparc reference UPI00000C7226, Uniprot reference Q9A9K2.

[1165] Seq ID No. 1019: Amino acid sequence of Bacteroides thetaiotaomicron rhamnosidase, Uniparc reference UPI000005BA09, Uniprot reference Q8A076.

[1166] Seq ID No. 1020: Amino acid sequence of: Rhodonellum psychrophilum rhamnosidase, Uniparc reference UPI0003745394, Uniprot reference U5BUY4.

[1167] Seq ID No. 1021: Amino acid sequence of Paenibacillus sp. rhamnosidase, Uniparc reference UPI0004F6D660, Uniprot reference A0A089M3T2.

[1168] Seq ID No. 1022: Amino acid sequence of Catenovulum agarivorans rhamnosidase, Uniparc reference UPI0003ED860D, Uniprot reference W7QMH5.

[1169] Seq ID No. 1023: Amino acid sequence of Zobellia galactanivorans rhamnosidase, Uniparc reference UPI000217D8B1, Uniprot reference G0L382.

[1170] Seq ID No. 1024: Amino acid sequence of Bacteroides thetaiotaomicron rhamnosidase, Uniparc reference UPI000005B845, Uniprot reference Q8A1H5.

[1171] Seq ID No. 1025: Amino acid sequence of Bacteroides xylanisolvens rhamnosidase, Uniparc reference UPI0001CD02E9, Uniprot reference D6CYE5.

[1172] Seq ID No. 1026: Amino acid sequence of: Pseudarthrobacter chlorophenolicus rhamnosidase, Uniparc reference UPI00018E07C6, Uniprot reference B8HAH3.

[1173] Seq ID No. 1027: Amino acid sequence of the following: Dictyoglomus thermophilum rhamnosidase, Uniparc reference UPI0001815896, Uniprot reference B5YC64.

[1174] Seq ID No. 1028: Amino acid sequence of Formosa agariphila rhamnosidase, Uniparc reference UPI00039231C1, Uniprot reference T2KPL4.

[1175] Seq IID No. 1029: Amino acid sequence of: Rhodococcus jostii rhamnosidase, Uniparc reference UPI0000DBA6EB, Uniprot reference Q0S9T4.

[1176] Seq ID No. 1030: Amino acid sequence of Lactobacillus crispatus rhamnosidase, Uniparc reference UPI0001D10896, Uniprot reference D5GZ45.

[1177] Seq ID No. 1031: Amino acid sequence of Pedobacter heparinus rhamnosidase, Uniparc reference UPI0001B17DE6, Uniprot reference C6XVU2.

[1178] Seq ID No. 1032: Amino acid sequence of Spirosoma linguale rhamnosidase, Uniparc reference UPI0001A3C989, Uniprot reference D2QUA5.

[1179] Seq ID No. 1033: Amino acid sequence of: Pedobacter heparinus rhamnosidase, Uniparc reference UPI00019EE3EB, Uniprot reference C6XU05.

[1180] Seq ID No. 1034: Amino acid sequence of Paenibacillus mucilaginosus rhamnosidase, Uniparc reference UPI0003432C11, Uniprot reference R9ULQ4.

[1181] Seq ID No. 1035: Amino acid sequence of the following: Isolated Caulobacter segnis rhamnosidase, Uniparc reference UPI0001BC0C05, Uniprot reference D5VGD9.

[1182] Seq ID No. 1036: Amino acid sequence of Bacteroides cellulosilyticus rhamnosidase, Uniparc reference UPI0001969377, Uniprot reference E2N9B1.

[1183] Seq ID No. 1037: Amino acid sequence of the following: Pedobacter heparinus rhamnosidase, Uniparc reference UPI0001B17C60, Uniprot reference C6Y153.

[1184] SeqID No. 1038: Amino acid sequence of Formosa agariphila rhamnosidase, Uniparc reference UPI000571C0C2, Uniprot reference T2KNB2.

[1185] Seq ID No. 1039: Amino acid sequence of Lactobacillus acidophilus rhamnosidase, Uniparc reference UPI00004C6D41, Uniprot reference Q5FJ31.

[1186] Seq ID No. 1040: Amino acid sequence of: Rhodopirellula baltica rhamnosidase, Uniparc reference UPI00001AC07D, Uniprot reference Q7UYD5.

[1187] Seq ID No. 1041: Amino acid sequence of: Frankia inefficax rhamnosidase, Uniparc reference UPI0001BF9A6C, Uniprot reference E3IY10.

[1188] Seq ID No. 1042: Amino acid sequence of Streptomyces scabiei rhamnosidase, Uniparc reference UPI0001B7FF91, Uniprot reference C9Z376.

[1189] Seq ID No. 1043: Amino acid sequence of: Flavobacterium johnsoniae rhamnosidase, Uniparc reference UPI00006E5F74, Uniprot reference A5FC22.

[1190] Seq ID No. 1044: Amino acid sequence of Streptomyces sp. rhamnosidase, Uniparc reference UPI00034E666D, Uniprot reference S2YWB5.

[1191] Seq ID No. 1045: Amino acid sequence of: Acidobacterium capsulatum rhamnosidase, Uniparc reference UPI000198DF25, Uniprot reference C1F149.

[1192] Seq ID No. 1046: Amino acid sequence of Catenovulum agarivorans rhamnosidase, Uniparc reference UPI0003ED82D3, Uniprot reference W7QYP5.

[1193] Seq ID No. 1047: Amino acid sequence of Brachybacterium faecium rhamnosidase, Uniparc reference UPI0001A461B7, Uniprot reference C7MA59.

[1194] Seq ID No. 1048: Amino acid sequence of the following: Klebsiella oxytoca rhamnosidase, Uniparc reference UPI000243A177, Uniprot reference A0A0J9X262.

[1195] Seq ID No. 1049: Amino acid sequence of the following: Chitinophaga pinensis rhamnosidase, Uniparc reference UPI0001B24769, Uniprot reference C7PA70.

[1196] Seq ID No. 1050: Amino acid sequence of Streptomyces botropensis rhamnosidase, Uniparc reference UPI0002BCAF6B, Uniprot reference M3FYL9.

[1197] Seq ID No. 1051: Amino acid sequence of: Subdoligranulum variabile rhamnosidase, Uniparc reference UPI0001966B28, Uniprot reference D1PKC7.

[1198] Seq ID No. 1052: Amino acid sequence of Microbacterium testaceum rhamnosidase, Uniparc reference UPI0001F8A51D, Uniprot reference E8NDD8.

[1199] Seq ID No. 1053: Amino acid sequence of: Solibacter usitatus rhamnosidase, Uniparc reference UPI000053767B, Uniprot reference Q01V09.

[1200] Seq ID No. 1054: Amino acid sequence of Streptosporangium roseum rhamnosidase, Uniparc reference UPI0001A3EFE6, Uniprot reference D2B240.

[1201] Seq ID No. 1055: Amino acid sequence of: Alphaproteobacterium) rhamnosidase, Uniparc reference UPI0006CE1E82, Uniprot reference A0A0N1BME3.

[1202] Seq ID No. 1056: Amino acid sequence of: Solidea canadensis rhamnosidase, Uniparc reference UPI000247229E, Uniprot reference H8KPI7.

[1203] Seq ID No. 1057: Amino acid sequence of Parabacteroides goldsteinii rhamnosidase, Uniparc reference UPI0002CB9583, Uniprot reference S0GSF0.

[1204] Seq ID No. 1058: Amino acid sequence of Cyclobacterium marinum rhamnosidase, Uniparc reference UPI00021B9B33, Uniprot reference G0J630.

[1205] Seq ID No. 1059: Amino acid sequence of: Solibacter usitatus rhamnosidase, Uniparc reference UPI0000533669, Uniprot reference Q01TX2.

[1206] Seq ID No. 1060: Amino acid sequence of Lunatimonas lonarensis rhamnosidase, Uniparc reference UPI00032D8F6D, Uniprot reference R7ZW70.

[1207] Seq ID No. 1061: Amino acid sequence of Rhizobium leguminosarum rhamnosidase, Uniparc reference UPI0000D713F2, Uniprot reference Q1M7P3.

[1208] Seq ID No. 1062: Amino acid sequence of Streptosporangium roseum rhamnosidase, Uniparc reference UPI0001A3EBEB, Uniprot reference D2AYU9.

[1209] Seq ID No. 1063: Amino acid sequence of Parabacteroides distasonis rhamnosidase, Uniparc reference UPI000156F115, Uniprot reference A6LBL4.

[1210] Seq ID No. 1064: Amino acid sequence of the following: Lachnospiraceae bacterium rhamnosidase, Uniparc reference UPI0003375A10, Uniprot reference R9K6L6.

[1211] Seq ID No. 1065: Amino acid sequence of the following: Chitinophaga pinensis rhamnosidase, Uniparc reference UPI0001A2F0FA, Uniprot reference C7P9Y8.

[1212] Seq ID No. 1066: Amino acid sequence of the following: Isolated Caulobacter segnis rhamnosidase, Uniparc reference UPI0001BC0C15, Uniprot reference D5VGC3.

[1213] Seq ID No. 1067: Amino acid sequence of the following: Pedobacter heparinus rhamnosidase, Uniparc reference UPI00019EF6E1, Uniprot reference C6Y145.

[1214] Seq ID No. 1068: Amino acid sequence of the following: Pedobacter heparinus rhamnosidase, Uniparc reference UPI00019EE1A9, Uniprot reference C6Y2X3.

[1215] Seq ID No. 1069: Amino acid sequence of: Deltaproteobacteriabacterium rhamnosidase, Uniparc reference UPI000C8D4928, Uniprot reference A0A2D5SK32.

[1216] Seq ID No. 1070: Amino acid sequence of Thermobaculum terrenum rhamnosidase, Uniparc reference UPI00019BFDCE, Uniprot reference D1CHL4. Seq ID No. 1071: Amino acid sequence of Opitutus terrae rhamnosidase, Uniparc reference UPI000172B62A, Uniprot reference B1ZY35.

[1217] Seq ID No. 1072: Amino acid sequence of the following: Kribbella flavida rhamnosidase, Uniparc reference UPI00019BFABB, Uniprot reference D2PXQ4.

[1218] Seq ID No. 1073: Amino acid sequence of Streptomyces scabiei rhamnosidase, Uniparc reference UPI0001B80091, Uniprot reference C9Z391.

[1219] Seq ID No. 1074: Amino acid sequence of Actinoplanes sp. rhamnosidase, Uniparc reference UPI00023EC5D1, Uniprot reference G8S540.

[1220] Seq ID No. 1075: Amino acid sequence of: Asticcacaulis sp. rhamnosidase, Uniparc reference UPI0003C3CD2B, Uniprot reference V4NSJ1.

[1221] Seq ID No. 1076: Amino acid sequence of the following: Kribbella flavida rhamnosidase, Uniparc reference UPI00019BF65D, Uniprot reference D2PT74.

[1222] Seq ID No. 1077: Amino acid sequence of Bacillus sp. rhamnosidase, Uniparc reference UPI00000BC760, Uniprot reference Q93RE7.

[1223] Seq ID No. 1078: Amino acid sequence of: Flavobacterium johnsoniae rhamnosidase, Uniparc reference UPI00006E5FAB, Uniprot reference A5FCG3.

[1224] Seq ID No. 1079: Amino acid sequence of Lunatimonas lonarensis rhamnosidase, Uniparc reference UPI00032EEB9C, Uniprot reference R7ZS84.

[1225] Seq ID No. 1080: Amino acid sequence of Eisenbergiella massiliensis rhamnosidase, Uniparc reference UPI0004B2D794, Uniprot reference A0A3E3IGR6.

[1226] Seq ID No. 1081: Amino acid sequence of Catenovulum agarivorans rhamnosidase, Uniparc reference UPI0003ED7515, Uniprot reference W7QF25.

[1227] Seq ID No. 1083: Codon-optimized DNA encoding 6xHis Rhodothermus marinus rhamnosidase, Uniparc reference UPI0001A31108, Uniprot reference DOMFR0.

[1228] Seq ID No. 1084: Codon-optimized DNA encoding 6xHis Streptomyces bingchenggensis rhamnosidase, Uniparc reference UPI0001D90BFE, Uniprot reference D7C463.

[1229] Seq ID No. 1085: Codon-optimized DNA encoding 6xHis Spirosomalinguale rhamnosidase, Uniparc reference UPI0001A3AEAC, Uniprot reference D2QL60.

[1230] Seq ID No. 1086: Codon-optimized DNA encoding 6xHis Roseburia intestinalis rhamnosidase, Uniparc reference UPI0001CD6D48, Uniprot reference D4L2K8.

[1231] Seq ID No. 1087: Codon-optimized DNA encoding 6xHis Draconibacterium orientale rhamnosidase, Uniparc reference UPI000442EF22, Uniprot reference X5DG83.

[1232] Seq ID No. 1088: Codon-optimized DNA encoding 6xHis Catenulispora acidiphila rhamnosidase, Uniparc reference UPI00019E052B, Uniprot reference C7QC24.

[1233] Seq ID No. 1089: Codon-optimized DNA encoding 6xHis Bacteroides thetaiotaomicron rhamnosidase, Uniparc reference UPI000005ADE1, Uniprot reference Q8A916.

[1234] Seq ID No. 1090: Codon-optimized DNA encoding 6xHis Opitutus terrae rhamnosidase, Uniparc reference UPI000172B2E3, Uniprot reference B1ZRE4.

[1235] Seq ID No. 1091: Codon-optimized DNA encoding 6xHis Lachnoclostridium phytofermentans rhamnosidase, Uniparc reference UPI00015FE0BE, Uniprot reference A9KJP8.

[1236] Seq ID No. 1092: Codon-optimized DNA encoding 6xHis Rhodanobacter denitrificans rhamnosidase, Uniparc reference UPI00022DA3ED, Uniprot reference M4NH01.

[1237] Seq ID No. 1093: Codon-optimized DNA encoding 6xHis Prevotella ruminicola rhamnosidase, Uniparc reference UPI0001D07633, Uniprot reference D5ETD9.

[1238] Seq ID No. 1094: Codon-optimized DNA encoding 6xHis Aspergillus terreus rhamnosidase, Uniparc reference UPI000259E388, Uniprot reference I0AZ41.

[1239] Seq ID No. 1095: Codon-optimized DNA encoding 6xHis Brachybacterium faecium rhamnosidase, Uniparc reference UPI0001A461B6, Uniprot reference C7MA58.

[1240] Seq ID No. 1096: Codon-optimized DNA encoding 6xHis Flavobacterium johnsoniae rhamnosidase, Uniparc reference UPI00006E4A0D, Uniprot reference A5FCH3.

[1241] Seq ID No. 1097: Codon-optimized DNA encoding 6xHis Rahnella aquatilis rhamnosidase, Uniparc reference UPI000245C507, Uniprot reference H2IYR2.

[1242] Seq ID No. 1098: Codon-optimized DNA encoding 6xHis Bifidobacterium moukalabense rhamnosidase, Uniparc reference UPI0003E4E711, Uniprot reference W4N6H0.

[1243] Seq ID No. 1099: Codon-optimized DNA encoding 6xHis Enterococcus casseliflavus rhamnosidase, Uniparc reference UPI000353BD9F, Uniprot reference S4BBS9.

[1244] Seq ID No. 1100: Codon-optimized DNA encoding 6xHis Geobacillus sp. rhamnosidase, Uniparc reference UPI0001789C0D, Uniprot reference D3EED1.

[1245] Seq ID No. 1101: Codon-optimized DNA encoding 6xHis marinotrophic rhamnosidase, Uniparc reference UPI000260A2FE, Uniprot reference I4EYD9.

[1246] Seq ID No. 1102: Codon-optimized DNA encoding 6xHis Pedobacter heparinus rhamnosidase, Uniparc reference UPI00019EF0EE, Uniprot reference C6XYM6.

[1247] Seq ID No. 1103: Codon-optimized DNA encoding 6xHis Dyadobacterfermentans rhamnosidase, Uniparc reference UPI00019B5915, Uniprot reference C6VZL3.

[1248] Seq ID No. 1104: Codon-optimized DNA encoding 6xHis Paenibacillus mucilaginosus rhamnosidase, Uniparc reference UPI000212C156, Uniprot reference F8FQQ3.

[1249] Seq ID No. 1105: Codon-optimized DNA encoding 6xHis Paenibacillus sp. rhamnosidase, Uniparc reference UPI0003 E2544D, Uniprot reference W4D866.

[1250] Seq ID No. 1106: Codon-optimized DNA encoding 6xHis Bacteroides thetaiotaomicron rhamnosidase, Uniparc reference UPI000005ADD2, Uniprot reference A0A0P0FM19.

[1251] Seq ID No. 1107: Codon-optimized DNA encoding 6xHis Chloroflexus aurantiacus rhamnosidase, Uniparc reference UPI00005BA60B, Uniprot reference A9WDK5.

[1252] Seq ID No. 1108: Codon-optimized DNA encoding 6xHis Thermoclostridium stercorarium rhamnosidase, Uniparc reference UPI00000B098C, Uniprot reference Q9S3L0.

[1253] Seq ID No. 1109: Codon-optimized DNA encoding 6xHis Bifidobacterium moukalabense rhamnosidase, Uniparc reference UPI0003E5C314, Uniprot reference W4N6I2.

[1254] Seq ID No. 1110: Codon-optimized DNA encoding 6xHis Olsenella profusa rhamnosidase, Uniparc reference UPI0003AE032C, Uniprot reference U2USP4.

[1255] Seq ID No. 1111: Codon-optimized DNA encoding 6xHis Kribbellaflavida rhamnosidase, Uniparc reference UPI00019BDB13, Uniprot reference D2PMT5.

[1256] Seq ID No. 1112: Codon-optimized DNA encoding 6xHis Caulobacter vibrioides rhamnosidase, Uniparc reference UPI00000C7226, Uniprot reference Q9A9K2.

[1257] Seq ID No. 1113: Codon-optimized DNA encoding 6xHis Bacteroides thetaiotaomicron rhamnosidase, Uniparc reference UPI000005BA09, Uniprot reference Q8A076.

[1258] Seq ID No. 1114: Codon-optimized DNA encoding 6xHis Rhodonellum psychrophilum rhamnosidase, Uniparc reference UPI0003745394, Uniprot reference U5BUY4.

[1259] Seq ID No. 1115: Codon-optimized DNA encoding 6xHis Paenibacillus sp. rhamnosidase, Uniparc reference UPI0004F6D660, Uniprot reference A0A089M3T2.

[1260] Seq ID No. 1116: Codon-optimized DNA encoding 6xHis Catenovulumagarivorans rhamnosidase, Uniparc reference UPI0003ED860D, Uniprot reference W7QMH5.

[1261] Seq ID No. 1117: Codon-optimized DNA encoding 6xHis rhamnosidase from Zobellia galactanivorans, Uniparc reference UPI000217D8B1, Uniprot reference G0L382.

[1262] Seq ID No. 1118: Codon-optimized DNA encoding 6xHis Bacteroides thetaiotaomicron rhamnosidase, Uniparc reference UPI000005B845, Uniprot reference Q8A1H5.

[1263] Seq ID No. 1119: Codon-optimized DNA encoding 6xHis Bacteroides xylanisolvens rhamnosidase, Uniparc reference UPI0001CD02E9, Uniprot reference D6CYE5.

[1264] Seq ID No. 1120: Codon-optimized DNA encoding 6xHis Pseudarthrobacter chlorophenolicus rhamnosidase, Uniparc reference UPI00018E07C6, Uniprot reference B8HAH3.

[1265] Seq ID No. 1121: Codon-optimized DNA encoding 6xHis Dictyoglomus thermophilum rhamnosidase, Uniparc reference UPI0001815896, Uniprot reference B5YC64.

[1266] Seq ID No. 1122: Codon-optimized DNA encoding 6xHis Formosa agariphila rhamnosidase, Uniparc reference UPI00039231Cl, Uniprot reference T2KPL4.

[1267] Seq ID No. 1123: Codon-optimized DNA encoding 6xHis Rhodococcus jostii rhamnosidase, Uniparc reference UPI0000DBA6EB, Uniprot reference Q0S9T4.

[1268] Seq ID No. 1124: Codon-optimized DNA encoding 6xHis Lactobacillus crispatus rhamnosidase, Uniparc reference UPI0001D10896, Uniprot reference D5GZ45.

[1269] Seq ID No. 1125: Codon-optimized DNA encoding 6xHis Pedobacter heparinus rhamnosidase, Uniparc reference UPI0001B17DE6, Uniprot reference C6XVU2.

[1270] Seq ID No. 1126: Codon-optimized DNA encoding 6xHis Spirosomalinguale rhamnosidase, Uniparc reference UPI0001A3C989, Uniprot reference D2QUA5.

[1271] Seq ID No. 1127: Codon-optimized DNA encoding 6xHis Pedobacter heparinus rhamnosidase, Uniparc reference UPI00019EE3EB, Uniprot reference C6XU05.

[1272] Seq ID No. 1128: Codon-optimized DNA encoding 6xHis Paenibacillus mucilaginosus rhamnosidase, Uniparc reference UPI0003432C11, Uniprot reference R9ULQ4.

[1273] Seq ID No. 1129: Codon-optimized DNA encoding the 6xHis isolated Caulobacter segnis rhamnosidase, Uniparc reference UPI0001BC0C05, Uniprot reference D5VGD9.

[1274] Seq ID No. 1130: Codon-optimized DNA encoding 6xHis Bacteroides cellulosilyticus rhamnosidase, Uniparc reference UPI0001969377, Uniprot reference E2N9B1.

[1275] Seq ID No. 1131: Codon-optimized DNA encoding 6xHis Pedobacter heparinus rhamnosidase, Uniparc reference UPI0001B17C60, Uniprot reference C6Y153.

[1276] Seq ID No. 1132: Codon-optimized DNA encoding 6xHis Formosa agariphila rhamnosidase, Uniparc reference UPI000571C0C2, Uniprot reference T2KNB2.

[1277] Seq ID No. 1133: Codon-optimized DNA encoding 6xHis Lactobacillus acidophilus rhamnosidase, Uniparc reference UPI00004C6D41, Uniprot reference Q5FJ31.

[1278] Seq ID No. 1134: Codon-optimized DNA encoding 6xHis Rhodopirellula baltica rhamnosidase, Uniparc reference UPI00001AC07D, Uniprot reference Q7UYD5.

[1279] Seq ID No. 1135: Codon-optimized DNA encoding 6xHis Frankia inefficax rhamnosidase, Uniparc reference UPI0001BF9A6C, Uniprot reference E3IY10.

[1280] Seq ID No. 1136: Codon-optimized DNA encoding 6xHis Streptomyces cesscabiei rhamnosidase, Uniparc reference UPI0001B7FF91, Uniprot reference C9Z376.

[1281] Seq ID No. 1137: Codon-optimized DNA encoding 6xHis Flavobacterium johnsoniae rhamnosidase, Uniparc reference UPI00006E5F74, Uniprot reference A5FC22.

[1282] Seq ID No. 1138: Codon-optimized DNA encoding 6xHis Streptomyces sp. rhamnosidase, Uniparc reference UPI00034E666D, Uniprot reference S2YWB5.

[1283] Seq ID No. 1139: Codon-optimized DNA encoding 6xHis Acidobacterium capsulatum rhamnosidase, Uniparc reference UPI000198DF25, Uniprot reference C1F149.

[1284] Seq ID No. 1140: Codon-optimized DNA encoding 6xHis Catenovulumagarivorans rhamnosidase, Uniparc reference UPI0003ED82D3, Uniprot reference W7QYP5.

[1285] Seq ID No. 1141: Codon-optimized DNA encoding 6xHis Brachybacterium faecium rhamnosidase, Uniparc reference UPI0001A461B7, Uniprot reference C7MA59.

[1286] Seq ID No. 1142: Codon-optimized DNA encoding 6xHis Klebsiella oxytoca rhamnosidase, Uniparc reference UPI000243A177, Uniprot reference A0A0J9X262.

[1287] Seq ID No. 1143: Codon-optimized DNA encoding 6xHis Chitinophagapinensis rhamnosidase, Uniparc reference UPI0001B24769, Uniprot reference C7PA70.

[1288] Seq ID No. 1144: Codon-optimized DNA encoding 6xHis Streptomyces botropensis rhamnosidase, Uniparc reference UPI0002BCAF6B, Uniprot reference M3FYL9.

[1289] Seq ID No. 1145: Codon-optimized DNA encoding 6xHis variant Subdoligranulum variabile rhamnosidase, Uniparc reference UPI0001966B28, Uniprot reference D1PKC7.

[1290] Seq ID No. 1146: Codon-optimized DNA encoding 6xHis Microbacterium testaceum rhamnosidase, Uniparc reference UPI0001F8A51D, Uniprot reference E8NDD8.

[1291] Seq ID No. 1147: Codon-optimized DNA encoding 6xHis Solibacter usitatus rhamnosidase, Uniparc reference UPI000053767B, Uniprot reference Q01V09.

[1292] Seq ID No. 1148: Codon-optimized DNA encoding 6xHis Streptosporangium roseum rhamnosidase, Uniparc reference UPI0001A3EFE6, Uniprot reference D2B240.

[1293] Seq ID No. 1149: Codon-optimized DNA encoding 6xHis (α-Proteobacterium) rhamnosidase, Uniparc reference UPI0006CE1E82, Uniprot reference A0A0N1BME3.

[1294] Seq ID No. 1150: Codon-optimized DNA encoding 6xHis Solitalea canadensis rhamnosidase, Uniparc reference UPI000247229E, Uniprot reference H8KPI7.

[1295] Seq ID No. 1151: Codon-optimized DNA encoding 6xHis Parabacteroides goldsteinii rhamnosidase, Uniparc reference UPI0002CB9583, Uniprot reference S0GSF0.

[1296] Seq ID No. 1152: Codon-optimized DNA encoding 6xHis Cyclobacterium marinum rhamnosidase, Uniparc reference UPI00021B9B33, Uniprot reference G0J630.

[1297] Seq ID No. 1153: Codon-optimized DNA encoding 6xHis Solibacter usitatus rhamnosidase, Uniparc reference UPI0000533669, Uniprot reference Q01TX2.

[1298] Seq ID No. 1154: Codon-optimized DNA encoding 6xHis Lunatimonas lonarensis rhamnosidase, Uniparc reference UPI00032D8F6D, Uniprot reference R7ZW70.

[1299] Seq ID No. 1155: Codon-optimized DNA encoding 6xHis Rhizobium leguminosarum rhamnosidase, Uniparc reference UPI0000D713F2, Uniprot reference Q1M7P3.

[1300] Seq ID No. 1156: Codon-optimized DNA encoding 6xHis Streptosporangium roseum rhamnosidase, Uniparc reference UPI0001A3EBEB, Uniprot reference D2AYU9.

[1301] Seq ID No. 1157: Codon-optimized DNA encoding 6xHis Parabacteroides distasonis rhamnosidase, Uniparc reference UPI000156F115, Uniprot reference A6LBL4.

[1302] Seq ID No. 1158: Codon-optimized DNA encoding 6xHis rhamnosidase from Lachnospiraceae bacterium, Uniparc reference UPI0003375A10, Uniprot reference R9K6L6.

[1303] Seq ID No. 1159: Codon-optimized DNA encoding 6xHis Chitinophagapinensis rhamnosidase, Uniparc reference UPI0001A2F0FA, Uniprot reference C7P9Y8.

[1304] Seq ID No. 1160: Codon-optimized DNA encoding the 6xHis isolated Caulobacter segnis rhamnosidase, Uniparc reference UPI0001BC0C15, Uniprot reference D5VGC3.

[1305] Seq ID No. 1161: Codon-optimized DNA encoding 6xHis Pedobacter heparinus rhamnosidase, Uniparc reference UPI00019EF6E1, Uniprot reference C6Y145.

[1306] Seq ID No. 1162: Codon-optimized DNA encoding 6xHis Pedobacter heparinus rhamnosidase, Uniparc reference UPI00019EE1A9, Uniprot reference C6Y2X3.

[1307] Seq ID No. 1163: Codon-optimized DNA encoding 6xHis Deltaproteobacteria bacterium rhamnosidase, Uniparc reference UPI000C8D4928, Uniprot reference A0A2D5SK32.

[1308] Seq ID No. 1164: Codon-optimized DNA encoding 6xHis Thermobaculum terrenum rhamnosidase, Uniparc reference UPI00019BFDCE, Uniprot reference D1CHL4.

[1309] Seq ID No. 1165: Codon-optimized DNA encoding 6xHis Opitutus terrae rhamnosidase, Uniparc reference UPI000172B62A, Uniprot reference B1ZY35.

[1310] Seq ID No. 1166: Codon-optimized DNA encoding 6xHis Kribbellaflavida rhamnosidase, Uniparc reference UPI00019BFABB, Uniprot reference D2PXQ4.

[1311] Seq ID No. 1167: Codon-optimized DNA encoding 6xHis Streptomyces cesscabiei rhamnosidase, Uniparc reference UPI0001B80091, Uniprot reference C9Z391.

[1312] Seq ID No. 1168: Codon-optimized DNA encoding 6xHis Actinoplanes sp. rhamnosidase, Uniparc reference UPI00023EC5D1, Uniprot reference G8S540.

[1313] Seq ID No. 1169: Codon-optimized DNA encoding 6xHis Asticcacaulis sp. rhamnosidase, Uniparc reference UPI0003C3CD2B, Uniprot reference V4NSJ1.

[1314] Seq ID No. 1170: Codon-optimized DNA encoding 6xHis Kribbellaflavida rhamnosidase, Uniparc reference UPI00019BF65D, Uniprot reference D2PT74.

[1315] Seq ID No. 1171: Codon-optimized DNA encoding 6xHis Bacillus sp. rhamnosidase, Uniparc reference UPI00000BC760, Uniprot reference Q93RE7.

[1316] Seq ID No. 1172: Codon-optimized DNA encoding 6xHis Flavobacterium johnsoniae rhamnosidase, Uniparc reference UPI00006E5FAB, Uniprot reference A5FCG3.

[1317] Seq ID No. 1173: Codon-optimized DNA encoding 6xHis Lunatimonas lonarensis rhamnosidase, Uniparc reference UPI00032EEB9C, Uniprot reference R7ZS84.

[1318] Seq ID No. 1174: Codon-optimized DNA encoding 6xHis EisenbergieIla massiliensis rhamnosidase, Uniparc reference UPI0004B2D794, Uniprot reference A0A3E3IGR6.

[1319] Seq ID No. 1175: Codon-optimized DNA encoding 6xHis Catenovulumagarivorans rhamnosidase, Uniparc reference UPI0003ED7515, Uniprot reference W7QF25.

[1320] Seq ID No. 1176: Codon-optimized DNA encoding 6xHis Streptomyces avermitilis rhamnosidase, Uniparc reference UPI0000184198, Uniprot reference Q82PP4.

[1321] Seq ID No.1177: N-terminal His-tag / linker

[1322] Seq ID No.1178: C-terminal linker / His tag

[1323] Seq ID No. 1179: The following amino acid sequence: variant G1

[1324] Seq ID No. 1180: The following amino acid sequence: variant G2

[1325] Seq ID No. 1181: The following amino acid sequence: variant G3

[1326] Seq ID No. 1182: The following amino acid sequence: variant G4

[1327] Seq ID No. 1183: The following amino acid sequence: variant G5

[1328] Seq ID No. 1184: Codon-optimized DNA encoding the N-terminal 6xHis variant G1.

[1329] Seq ID No. 1185: Codon-optimized DNA encoding the N-terminal 6xHis variant G2.

[1330] Seq ID No. 1186: Codon-optimized DNA encoding the N-terminal 6xHis variant G3.

[1331] Seq ID No. 1187: Codon-optimized DNA encoding the N-terminal 6xHis variant G4.

[1332] Seq ID No. 1188: Codon-optimized DNA encoding the N-terminal 6xHis variant G5.

[1333] Seq ID No. 1189: The following amino acid sequence: variant R1

[1334] Seq ID No. 1190: The following amino acid sequence: variant R2

[1335] Seq ID No. 1191: The following amino acid sequence: variant R3

[1336] Seq ID No. 1192: The following amino acid sequence: variant R4

[1337] Seq ID No. 1193: The following amino acid sequence: variant R5

[1338] Seq ID No. 1194: Codon-optimized DNA encoding the C-terminal 6xHis variant R1.

[1339] Seq ID No. 1195: Codon-optimized DNA encoding the C-terminal 6xHis variant R2.

[1340] Seq ID No. 1196: Codon-optimized DNA encoding the C-terminal 6xHis variant R3.

[1341] Seq ID No. 1197: Codon-optimized DNA encoding the C-terminal 6xHis variant R4.

[1342] Seq ID No. 1198: Codon-optimized DNA encoding the C-terminal 6xHis variant R5. DETAILED DESCRIPTION

[1343] As previously mentioned, saponin is a steroid or terpenoid glycoside with a wide range of uses. The method for obtaining some saponin of appropriate quantity and appropriate purity at present (for example, obtaining from plant cell culture) is limited. The inventors have surprisingly found that the saponin synthesized and produced by plant cell culture is enzymatically modified and can help to improve the accessibility of interested saponin and / or help to remove unwanted saponin components. Therefore, the invention provides the method for enzymatically modifying the saponin synthesized and produced by plant cell culture, the product prepared by this method, the purposes of the product and related aspects. In the method of the present invention, the starting saponin (i.e., by enzyme-modified saponin) is converted into the product saponin (i.e., the saponin produced by the enzymatic modification of the starting saponin).

[1344] The engineered glucosidase polypeptides disclosed herein can be used in the methods of the present invention for the enzymatic modification of saponins.

[1345] The engineered rhamnosidase polypeptides disclosed herein can be used in the methods of the present invention for the enzymatic modification of saponins.

[1346] Target

[1347] The methods of the present invention can be used to achieve a variety of goals, such as: (i) increasing the yield of a saponin of interest that can be obtained from a given starting material; (ii) broadening the range of starting materials suitable for obtaining a saponin of interest; and / or (iii) conveniently removing undesirable saponins from a saponin of interest. In the sense of the present invention, the term "starting material" refers to material derived from plant cell culture.

[1348] When the parent material supply of separating interested saponin was limited, it was obviously important to realize the maximum yield of interested saponin. Independent of the efficiency of extraction and separation process (usually being adapted to best separate the existing interested saponin), the present invention can be used for increasing the amount of the interested saponin that can obtain from given parent material. Other saponins that exist in the parent material are carried out enzymatic modification to form interested saponin, can increase the amount of obtainable saponin of interest by the interested saponin of enrichment parent material.

[1349] Saponin can be obtained from a wide range of sources. The existence of specific saponin in plant material and its level may depend on a series of factors, such as plant species, tissue, age, season, environmental conditions etc. Variation can also be observed between individual plants (such as trees) of the same species (see, for example, WO2018057031). Similarly, the level of specific saponin and the variation of its respective ratio can be observed in a kind of plant cell culture and another kind of plant cell culture. The burden relevant to extracting and / or separating the saponin of interest may mean that some potential sources of the saponin of interest (such as in vitro plant cell culture) are commercially infeasible, because the saponin of interest exists at a relatively low level. Therefore, other saponins present are enzymatically modified to form the saponin of interest and the scope of the feasible source for obtaining the saponin of interest can be expanded.

[1350] As is well known, different saponins may have different activity characteristics - positive / desirable activity and negative / undesirable activity. Some uses of saponin require a high degree of purification, and separating the saponin of interest from other saponins, especially those with similar structures or physical properties, may be troublesome. Enzymatic modification of such other saponins can change their physical properties, thereby helping to separate from the saponin of interest. Other uses of saponin may not require high purity itself, yet still need to remove or reduce the amount of a specific saponin component (or multiple components) in a saponin mixture without using loaded down with trivial details chromatography. Enzymatic modification can promote the removal or reduction of a specific saponin component in a saponin mixture, without the need for chromatographic means.

[1351] Saponins

[1352] The methods of the present invention require a starting saponin (i.e., a saponin to be enzymatically modified). The starting saponin can be a naturally occurring saponin (i.e., a steroid or terpenoid glycoside found in nature) or an artificially produced saponin (i.e., a steroid or terpenoid glycoside not found in nature).

[1353] In some embodiments, the starting saponin is a steroid glycoside. In other embodiments, the starting saponin is a terpenoid glycoside, particularly a triterpenoid glycoside.

[1354] The naturally occurring starting saponin may be a saponin synthesized by plant cells cultured in vitro, which is derived from a saponin-producing plant, such as the plants described below.

[1355] Naturally occurring starting saponins include those obtainable from, for example, those obtained from, the in vitro plant cell cultures of Gypsophilia, Saponaria (e.g., Saponaria vaccaria species, or Saponaria officinalis species) or Quillaja species (Bomford, 1992). Particularly interesting are the starting saponins obtainable from, for example, the in vitro plant cell cultures of Quillaja species. Especially interesting starting saponins include those obtained from the in vitro plant cell cultures of Quillaja angustifolia or Quillaja salsa. In one embodiment, the starting saponins are obtained from the in vitro plant cell cultures of Quillaja angustifolia. In one embodiment, the starting saponins are obtained from the in vitro plant cell cultures of Quillaja angustifolia.

[1356] In certain embodiments, the starting saponin is a sapogenin glycoside. In certain embodiments, the starting saponin is a phytolaccinic acid glycoside. In certain embodiments, the starting saponin is an echinocystic acid glycoside. In certain embodiments, the starting saponin is a 22-β-hydroxylated sapogenin glycoside. In certain embodiments, the starting saponin is a gypsogenin glycoside.

[1357] Analysis of aqueous / methanol extracts of Quillaja bark by liquid chromatography / mass spectrometry revealed over 100 saponins (Nyberg, 2000; Nyberg, 2003; Kite, 2004). Quillaja angustifolia extracts have also been described, with many of the saponin components in Quillaja angustifolia extracts corresponding to those found in Quillaja angustifolia extracts (Wallace, 2017; Wallace, 2019).

[1358] Specific starting and product saponins derived from sapogenin are described below, grouped by "families." Each family shares one or more common structural features that characterize it relative to other families. Individual components within each family also exhibit certain structural features that characterize it relative to other components within the family, including: xylose or rhamnose chemotypes—the presence of a xylose or rhamnose residue in the C3 sugar; A or B isomers—A has an acyl chain attached via the 4-position of D-fucose, and B has an acyl chain attached via the 3-position of D-fucose; and V1 and V2—the presence of a terminal apiose or xylose residue, respectively, in the C28 sugar (this terminal residue may also be absent in other members of the family). This article focuses on components that are typically present in aqueous extracts of Quillaja saponaria (e.g., its bark extract), but it should be understood that (i) other components of the family are also present and (ii) the proportions of different components within the family may vary between families and between different saponin sources (Kite, 2004). The specific extraction method used may also affect the proportions of the different components obtained. Although the saponin fraction has been extensively studied using Quillaja bark extract, it is believed that the saponin fraction synthesized and produced by plant cells cultured in vitro is identical to that derived from Quillaja.

[1359] Chromatographic techniques can be used to separate the A and B isomers. However, under suitable solvent conditions, these isomers will return to equilibrium ratios (see, for example, Cleland, 1996). Xylose and rhamnose chemotypes are usually closely eluted. According to chromatographic techniques, the rhamnose chemotype may form a small peak that is immediately before or overlaps with the main peak of the family.

[1360] Those skilled in the art will also recognize that the structure comprises an ionizable group and can exist in the form of a dissociated form or a salt where appropriate. The structure is typically shown as the glucuronate moiety in ionized form, and the molecular weight indicated is calculated directly from the ion of display (corresponding to the monoisotope m / z observed with negative mode electrospray mass spectrometry), however, all non-dissociated, dissociated and salt forms are intended to be encompassed by the definition cited. Desirable salts are pharmaceutically acceptable, but non-pharmaceutically acceptable salts are also useful in pharmaceutical manufacturing processes or non-pharmaceutical uses.

[1361] The starting saponins from Quillaja saponaria that can be obtained from plant cells cultured in vitro include:

[1362] - QS-18 family components (i.e. triterpene glycosides having β-O-glucopyranosylation at the C3 position of the L-rhamnose moiety of QS-21 family components), for example:

[1363] - "QS-18 2150A component", which is identified as Figure 2A triterpene glycoside that is part of the main peak of QS-18 and has an m / z of 2150 using negative mode electrospray mass spectrometry. The QS-18 2150A component is believed to be identified as peak 76 in Kite 2004 and corresponds to the A-isomer xylose chemotype structures B4 (apiose isomer) and B6 (xylose isomer) characterized in Nyberg 2000 and Nyberg 2003. The QS-18 2150A component may be composed of the following: QS-18 2150A V1 (i.e., apiose isomer)

[1364]

[1365] and / or QS-18 2150A V2 (i.e., xylose isomers):

[1366]

[1367] - "QS-18 2018A component", which is identified as Figure 2 A triterpene glycoside that is part of the main peak of QS-18 and has an m / z of 2018, as determined by negative mode electrospray mass spectrometry. The QS-18A 2018 component is believed to have been identified as peak 73 by Kite 2004 and corresponds to the A-isomer xylose chemotype structure B2 characterized by Nyberg 2000 and Nyberg 2003. The QS-18 2018A component may be composed of the following: QS-18 2018A

[1368]

[1369] - "QS-18 2164A component", which is identified as Figure 2 A triterpene glycoside that is part of the main peak of QS-18 and has an m / z of 2164 using negative mode electrospray mass spectrometry. The QS-18 2164A component is believed to have been identified as peak 74 in Kite 2004 and corresponds to the A-isomer rhamnose chemotype structures B3 (the apiose isomer) and B5 (the xylose isomer) characterized in Nyberg 2000 and Nyberg 2003. The QS-18 2164A component may be composed of the following: QS-18 2164A V1 (i.e., the apiose isomer)

[1370]

[1371] and / or QS-18 2164A V2 (i.e., xylose isomers):

[1372]

[1373] - "QS-18 2150B Fraction" is a triterpene glycoside with m / z 2150, as determined by negative mode electrospray mass spectrometry. The QS-18 2150B Fraction corresponds to the B-isomer xylose chemotype structures B4a (apiose isomer) and B6a (xylose isomer) characterized in Nyberg 2000 and Nyberg 2003. The QS-18 2150B Fraction may be composed of: QS-18 2150B V1 (i.e., apiose isomer)

[1374]

[1375] and / or QS-18 2150B V2 (i.e. xylose isomers):

[1376]

[1377] - "QS-18 2018B Fraction" is a triterpene glycoside with m / z 2018, as determined by negative mode electrospray mass spectrometry. QS-18 2018B Fraction corresponds to the B-isomer xylose chemotype structure B2a characterized in Nyberg 2000 and Nyberg 2003. QS-18 2018B Fraction may be composed of: QS-18 2018B

[1378]

[1379] - "QS-18 2164B Fraction" is a triterpene glycoside with m / z 2164, as determined by negative mode electrospray mass spectrometry. QS-182164B corresponds to the B-isomer rhamnose chemotype structures B3a (apiose isomer) and B5a (xylose isomer). QS-182164B Fraction may be composed of: QS-18 2164B V1 (i.e., apiose isomer)

[1380]

[1381] and / or QS-18 2164B V2 (i.e. xylose isomers):

[1382]

[1383] - deglucosylated-QS-17 family components (i.e., triterpene glycosides having α-O-rhamnosylation at the C2 position of the arabinofuranosyl moiety of QS-21 family components but lacking the glycosylation of QS-17 family components), for example:

[1384] - "Deglucosyl-QS-17 2134A Fraction" is a triterpene glycoside with m / z 2134 as determined by negative mode electrospray mass spectrometry. Deglucosyl-QS-17 2134A Fraction is believed to have been identified as peak 75 in Kite 2004 and corresponds to the A-isomer of the xylose chemotype. Deglucosyl-QS-17 2134A Fraction may be composed of: Deglucosyl-QS-17 2134A V1 (i.e., apiose isomer)

[1385]

[1386] and / or deglucosyl-QS-17 2134A V2 (i.e. xylose isomer):

[1387]

[1388] - "Deglucosyl-QS-17 2002A Fraction" is a triterpene glycoside with m / z 2002, as determined by negative mode electrospray mass spectrometry. Deglucosyl-QS-17 2002A Fraction corresponds to the A-isomer xylose chemotype and may be composed of: Deglucosyl-QS-17 2002A:

[1389]

[1390] - "Deglucosyl-QS-17 2148A Fraction" is a triterpene glycoside with m / z 2148 as determined by negative mode electrospray mass spectrometry. Deglucosyl-QS-17 2148A Fraction is believed to have been identified in Kite 2004 as peaks 70 and 72 and corresponds to the A-isomer rhamnose chemotype. Deglucosyl-QS-17 2148A Fraction may be composed of: Deglucosyl-QS-17 2148A V1 (i.e., apiose isomer)

[1391]

[1392] and / or deglucosyl-QS-17 2148A V2 (i.e. xylose isomer):

[1393]

[1394] - "Deglucosyl-QS-17 2134B fraction" is a triterpene glycoside with m / z 2134, as determined by negative mode electrospray mass spectrometry. Deglucosyl-QS-17 2134B fraction is believed to be identified as peak 67 in Kite 2004 and corresponds to the B-isomer of the xylose chemotype. Deglucosyl-QS-17 2134B fraction may be composed of: Deglucosyl-QS-17 2134B V1 (i.e., apiose isomer)

[1395]

[1396] and / or deglucosyl-QS-17 2134B V2 (i.e. xylose isomer):

[1397]

[1398] - "Deglucosyl-QS-17 2002B fraction" is a triterpene glycoside with m / z 2002, as determined by negative mode electrospray mass spectrometry. Deglucosyl-QS-17 2002B fraction corresponds to the B-isomer of the xylose chemotype and may be composed of: Deglucosyl-QS-17 2002B

[1399]

[1400] - "Deglucosyl-QS-17 2148B fraction" is a triterpene glycoside with m / z 2148 as determined by negative mode electrospray mass spectrometry. Deglucosyl-QS-17 2148B fraction was identified as peak 65 in Kite 2004 and corresponds to the B-isomer of the rhamnose chemotype. Deglucosyl-QS-17 2148B fraction may be composed of: Deglucosyl-QS-17 2148B V1 (i.e., apiose isomer)

[1401]

[1402] and / or deglucosyl-QS-17 2148B V2 (i.e. xylose isomer):

[1403]

[1404] - QS-17 family components (i.e. triterpene glycosides having β-O-glucopyranosylation at the C3 position of the L-rhamnose moiety and α-O-rhamnose moiety at the C2 position of the arabinofuranosyl moiety of QS-21 family components), for example:

[1405] - "QS-17 2296A component", which is identified as Figure 2 A triterpene glycoside that is part of the main peak of QS-17 and has an m / z of 2296 using negative mode electrospray mass spectrometry. The QS-17 2296A component is believed to be identified as peak 59 in Kite 2004 and corresponds to the A-isomer of the xylose chemotype structure QS-III. The QS-17 2296A component may be composed of the following: QS-17 2296A V1 (i.e., apiose isomer)

[1406]

[1407] and / or QS-17 2296A V2 (i.e., xylose isomers):

[1408]

[1409] - "QS-17 2164A component", which is identified as Figure 2 A triterpene glycoside that is part of the main peak of QS-17 and has an m / z of 2164 using negative mode electrospray mass spectrometry. The QS-17 2164A component is believed to be identified as peak 58 in Kite 2004 and corresponds to the A-isomer xylose chemotype. The QS-17 2164A component may be composed of the following: QS-17 2164A

[1410]

[1411] - "QS-17 2310A component", which is identified as Figure 2 A triterpene glycoside that is part of the main peak of QS-17 and has an m / z of 2310 using negative mode electrospray mass spectrometry. The QS-17 2310A component is believed to be identified as peak 57 in Kite 2004 and corresponds to the A-isomer of the rhamnose chemotype. The QS-17 2310A component may be composed of the following: QS-17 2310A V1 (i.e., apiose isomer)

[1412]

[1413] and / or QS-17 2310 A V2 (i.e. xylose isomers):

[1414]

[1415] - "QS-17 2296B Fraction" is a triterpene glycoside with m / z 2296, as determined by negative mode electrospray mass spectrometry. QS-17 2296B Fraction corresponds to the B-isomer of the xylose chemotype structure QS-III in Kite 2004. QS-17 2296B Fraction may be composed of: QS-17 2296B V1 (i.e., apiose isomer)

[1416]

[1417] and / or QS-17 2296B V2 (i.e. xylose isomers):

[1418]

[1419] - "QS-17 2164B Fraction" is a triterpene glycoside with m / z 2164 as determined by negative mode electrospray mass spectrometry. The QS-17 2164B fraction corresponds to the B-isomer xylose chemotype. The QS-17 2164B fraction may be composed of:

[1420]

[1421] - "QS-17 2310B Fraction" is a triterpene with m / z 2310, as determined by negative mode electrospray mass spectrometry. QS-17 2310B Fraction corresponds to the B-isomer of the rhamnose chemotype. QS-17 2310B Fraction may be composed of: QS-17 2310BV1 (i.e., apiose isomer)

[1422]

[1423] and / or QS-17 2310B V2 (i.e., xylose isomers);

[1424]

[1425] - Dearabinofuranosyl-QS-18 family components (i.e., triterpene glycosides with β-O-glucopyranosylation at the C3 position of the L-rhamnose moiety but lacking the arabinofuranosyl moiety of QS-21 family components). Dearabinofuranosyl-QS-18 family components are present in relatively low concentrations in extracts, meaning they have not been characterized in detail. Separation of dearabinofuranosyl-QS-18 family components from QS-21 family components can be challenging. Dearabinofuranosyl-QS-18 family components include:

[1426] -Dearabinofuranosyl-QS-18 2018A component (i.e. Figure 6A triterpene glycoside identified as part of the "2018 peak" in the UPLC-UV / MS method described herein. Suitably, the desarabinofuranosyl-QS-18 2018A component in the UPLC-UV / MS method described herein has a retention time of approximately 4.5 minutes, and the major component of the peak has an m / z of 2018 when using negative mode electrospray mass spectrometry. The desarabinofuranosyl-QS-18 2018A component can also be identified in the UPLC-UV method described herein with a retention time of approximately 5.8 minutes. In Kite 2004, the desarabinofuranosyl-QS-18 2018A component is believed to be identified as peak 90 and corresponds to the A-isomer of the xylose chemotype. The putative structure has been identified as the major desarabinofuranosyl-QS-18 2018A component by MS / MS. The dearabinofuranosyl-QS-18 2018A component may be composed of: dearabinofuranosyl-QS-18 2018A V1 (i.e., apiose isomer)

[1427]

[1428] and / or desarabinofuranosyl-QS-18 2018A V2 (i.e., xylose isomer):

[1429]

[1430] -Dearabinofuranosyl-QS-18 1886A component (i.e. Figure 6 A triterpene glycoside identified as part of the "2018 peak" in the UPLC-UV / MS method described herein. Suitably, the desarabinofuranosyl-QS-18 1886A fraction has a retention time of approximately 4.5 minutes and an m / z of 1886 in the UPLC-UV / MS method described herein using negative mode electrospray mass spectrometry. The desarabinofuranosyl-QS-18 1886A fraction and the corresponding A-isomer xylose chemotype can also be identified in the UPLC-UV method described herein with a retention time of approximately 5.8 minutes. The desarabinofuranosyl-QS-18 1886A fraction can be composed of:

[1431]

[1432] -Dearabinofuranosyl-QS-18 2032A component (i.e. Figure 6The triterpene glycoside identified as part of the "2018 peak" in the UPLC-UV / MS method described herein is preferably a triterpene glycoside identified as part of the "2018 peak". Suitably, the desarabinofuranosyl-QS-18 2032A component in the UPLC-UV / MS method described herein has a retention time of approximately 4.5 minutes and an m / z of 2032 using negative mode electrospray mass spectrometry. The desarabinofuranosyl-QS-18 2032A component corresponds to the α-isomer of the rhamnose chemotype and can also be identified in the UPLC-UV method described herein with a retention time of approximately 5.8 minutes. The desarabinofuranosyl-QS-18 2032A component may consist of: desarabinofuranosyl-QS-18 2032A V1 (i.e., the apiose isomer)

[1433]

[1434] and / or desarabinofuranosyl-QS-18 2032A V2 (i.e., xylose isomer):

[1435]

[1436] -Dearabinofuranosyl-QS-18 2018B fraction (i.e., triterpene glycoside, m / z 2018, using negative mode electrospray mass spectrometry). Dearabinofuranosyl-QS-18 2018B fraction corresponds to the B-isomer of the xylose chemotype. Dearabinofuranosyl-QS-18 2018B fraction can be composed of: Dearabinofuranosyl-QS-18 2018B V1 (i.e., apiose isomer)

[1437]

[1438] and / or desarabinofuranosyl-QS-18 2018B V2 (i.e., xylose isomer):

[1439]

[1440] - Desarabinofuranosyl-QS-18 1886B fraction (i.e., triterpene glycoside identified at m / z 1886 by negative mode electrospray mass spectrometry). Desarabinofuranosyl-QS-18 1886B fraction corresponds to the B-isomer xylose chemotype. Desarabinofuranosyl-QS-18 1886B fraction may be composed of:

[1441]

[1442] - Desarabinofuranosyl-QS-18 2032B fraction (i.e., triterpene glycoside, m / z 2032, using negative mode electrospray mass spectrometry). Desarabinofuranosyl-QS-18 2032B fraction corresponds to the B-isomer of the rhamnose chemotype. Desarabinofuranosyl-QS-18 2032B fraction may be composed of: Desarabinofuranosyl-QS-18 2032B V1 (i.e., apiose isomer)

[1443]

[1444] and / or desarabinofuranosyl-QS-18 2032B V2 (i.e., xylose isomer):

[1445]

[1446] - acetylated deglucosyl-QS-17 family components (i.e. triterpene glycosides with α-O-rhamnosylation at the C2 position of the arabinofuranosyl moiety and acetylation of the fucose at the C3 position of the QS-21 family components), for example:

[1447] - "Acetylated deglucosyl-QS-17 2176A fraction" is a triterpene glycoside with m / z 2176, as determined by negative mode electrospray mass spectrometry. Acetylated deglucosyl-QS-17 2176A fraction corresponds to the α-isomer of the xylose chemotype. Acetylated deglucosyl-QS-17 2176A fraction may be composed of: Acetylated deglucosyl-QS-17 2176A V1 (i.e., apiose isomer)

[1448]

[1449] and / or acetylated deglucosyl-QS-17 2176A V2 (i.e., xylose isomer):

[1450] - "Acetylated deglucosyl-QS-17 2044A fraction" is a triterpene glycoside with m / z 2044, as determined by negative mode electrospray mass spectrometry. Acetylated deglucosyl-QS-17 2044A fraction corresponds to the A-isomer xylose chemotype. Acetylated deglucosyl-QS-17 2044A fraction may be composed of: Acetylated deglucosyl-QS-17 2044A

[1451]

[1452] - "Acetylated deglucosyl-QS-17 2190A fraction" is a triterpene glycoside with m / z 2190, as determined by negative mode electrospray mass spectrometry. Acetylated deglucosyl-QS-17 2190A corresponds to the α-isomer rhamnose chemotype. Acetylated deglucosyl-QS-17 2190A fraction may be composed of: Acetylated deglucosyl-QS-17 2190A V1 (i.e., apiose isomer)

[1453]

[1454] and / or acetylated deglucosyl-QS-17 2190A V2 (i.e., xylose isomer):

[1455]

[1456] The starting saponins directly associated with the engineered glucosidase polypeptides used in the methods of the present invention are those having cleavable glucose residues, however, the engineered glucosidase polypeptides can be used in conjunction with additional enzymes capable of cleaving other sugar residues. Specific starting saponins associated with the engineered glucosidase polypeptides include:

[1457] -QS-18 family components;

[1458] - QS-17 family components; and

[1459] -Desarabinofuranosyl-QS-18 family components.

[1460] The starting saponins directly associated with the engineered rhamnosidase polypeptides used in the methods of the present invention are those having cleavable rhamnose residues, however, the engineered rhamnosidase polypeptides can be used in conjunction with additional enzymes capable of cleaving other sugar residues. Specific starting saponins associated with the engineered rhamnosidase polypeptides include:

[1461] - Deglucosyl QS-17 family components;

[1462] - QS-17 family components; and

[1463] -Acetylated deglucosyl QS-17 family components.

[1464] The methods of the present invention enzymatically modify starting saponins obtained from plant cell culture to provide product saponins (ie, saponins resulting from the enzymatic modification).

[1465] The product saponin may be a naturally occurring saponin (i.e., a steroid or terpenoid glycoside found in nature, even though the product saponin itself is obtained by the method of the present invention) or an artificially produced saponin (i.e., a steroid or terpenoid glycoside not found in nature).

[1466] In some embodiments, the product saponin is a steroid glycoside. In other embodiments, the product saponin is a terpenoid glycoside, particularly a triterpenoid glycoside.

[1467] Naturally occurring product saponins may be saponins synthesized by plant cells cultured in vitro, which are derived from saponin-producing plants, such as the plants described below.

[1468] Naturally occurring product saponins include those obtainable from, for example, those obtained from in vitro plant cell cultures of Gypsophilia, Saponaria (e.g., Saponaria vaccaria species, or Saponaria officinalis species) or Quillaja species (Bomford, 1992). Of particular interest are the starting saponins obtainable from in vitro plant cell cultures of Quillaja species. Especially interesting starting saponins include those obtained from in vitro plant cell cultures of Quillaja angustifolia or Quillaja salsa. In one embodiment, the starting saponins are obtained from in vitro plant cell cultures of Quillaja angustifolia. In one embodiment, the starting saponins are obtained from in vitro plant cell cultures of Quillaja angustifolia.

[1469] In certain embodiments, the product saponin is a sapogenin glycoside.

[1470] Saponins, products obtainable from plant cell culture derived from Quillaja saponaria, include:

[1471] - QS-18 family components (i.e. triterpene glycosides having β-O-glucopyranosylation at the C3 position of the L-rhamnose moiety of QS-21 family components), for example:

[1472] - "QS-18 2150A component". The QS-18 2150A component may be composed of: QS-18 2150A V1 (i.e., celery sugar isomers)

[1473]

[1474] and / or QS-18 2150A V2 (i.e., xylose isomers):

[1475]

[1476] - "QS-18 2018A component". A QS-18 2018A component may consist of: QS-18 2018A

[1477]

[1478] - "QS-18 2164A component". The QS-18 2164A component may consist of: QS-18 2164A V1 (i.e., celery sugar isomer)

[1479]

[1480] and / or QS-18 2164A V2 (i.e., xylose isomers):

[1481]

[1482] - "QS-18 2150B component". The QS-18 2150B component may be composed of: QS-18 2150B V1 (i.e., celery sugar isomer)

[1483]

[1484] and / or QS-18 2150B V2 (i.e. xylose isomers):

[1485]

[1486] - "QS-18 2018B component". The QS-18 2018B component may consist of: QS-18 2018B

[1487]

[1488] - "QS-18 2164B component". The QS-18 2164B component may consist of: QS-18 2164B V1 (i.e., celery sugar isomer)

[1489]

[1490] and / or QS-18 2164B V2 (i.e. xylose isomers):

[1491]

[1492] - deglucosyl-QS-17 family components (i.e. triterpene glycosides having α-O-rhamnosylation at the C2 position of the arabinofuranosyl moiety of the QS-21 family components), for example:

[1493] - "Deglucosyl QS-17 2134A fraction". The deglucosyl-QS-17 2134A fraction may be composed of: deglucosyl-QS-17 2134A V1 (i.e., celery sugar isomer)

[1494]

[1495] and / or deglucosyl-QS-17 2134A V2 (i.e. xylose isomer):

[1496]

[1497] - "Deglucosyl QS-17 2002A component". The deglucosyl-QS-17 2002A component may be composed of: deglucosyl-QS-17 2002A

[1498]

[1499] - "Deglucosyl QS-17 2148A fraction". The deglucosyl-QS-17 2148A fraction may be composed of: deglucosyl-QS-17 2148A V1 (i.e., celery sugar isomer)

[1500]

[1501] and / or deglucosyl-QS-17 2148A V2 (i.e. xylose isomer):

[1502]

[1503] - "Deglucosyl QS-17 2134B component". The deglucosyl-QS-17 2134B component may be composed of: Deglucosyl-QS-17 2134B V1 (i.e., celery sugar isomer):

[1504] and / or deglucosyl-QS-17 2134B V2 (i.e. xylose isomer):

[1505]

[1506] - "Deglucosyl QS-17 2002B component". The deglucosyl-QS-17 2002B component may be composed of: deglucosyl-QS-17 2002B

[1507]

[1508] - "Deglucosyl QS-17 2148B component". The deglucosyl-QS-17 2148B component may be composed of: deglucosyl-QS-17 2148B V1 (i.e., celery sugar isomer)

[1509]

[1510] and / or deglucosyl-QS-17 2148B V2 (i.e. xylose isomer):

[1511]

[1512] -QS-21 family components, such as:

[1513] - "QS-21 1988A component", which is identified as Figure 6 The triterpene glycosides are part of the QS-21 main peak in the UPLC-UV / MS method and have an m / z of 1988 using negative mode electrospray mass spectrometry. Suitably, the QS-21 1988A fraction in the UPLC-UV / MS method described herein has a retention time of approximately 4.4 minutes and an m / z of 1988 using negative mode electrospray mass spectrometry. The QS-21 1988A fraction is believed to be identified as peak 88 in Kite 2004 and corresponds to the A-isomer xylose chemotype structures S6 (apiose isomer) and S4 (xylose isomer) characterized in Nyberg 2000 and Nyberg 2003. The QS-21 1988A fraction may be composed of: QS-21 1988A V1 (i.e., apiose isomer)

[1514]

[1515] and QS-21 1988A V2 (i.e. xylose isomers):

[1516]

[1517] - "QS-21 1856A component", which is identified as Figure 6 The triterpene glycosides are part of the QS-21 main peak in the UPLC-UV / MS method and have an m / z of 1856 using negative mode electrospray mass spectrometry. Suitably, the QS-21 1856A fraction in the UPLC-UV / MS method described herein has a retention time of approximately 4.4 minutes and an m / z of 1856 using negative mode electrospray mass spectrometry. The QS-21 1856A fraction is believed to be identified as peak 86 by Kite 2004 and corresponds to the A-isomer xylose chemotype structure S2 characterized by Nyberg 2000 and Nyberg 2003. The QS-21 1856A fraction may be composed of:

[1518]

[1519] - "QS-21 2002A component", which is identified as Figure 6The triterpene glycosides are part of the QS-21 main peak in the UPLC-UV / MS method and have an m / z of 2002 using negative mode electrospray mass spectrometry. Suitably, the QS-21 2002A fraction in the UPLC-UV / MS method described herein has a retention time of approximately 4.4 minutes and an m / z of 2002 using negative mode electrospray mass spectrometry. The QS-21 2002A fraction is believed to be identified as peak 85 in Kite 2004 and corresponds to the α-isomer rhamnose chemotype of structures S3 and S5 characterized in Nyberg 2000 and Nyberg 2003. The QS-21 2002A fraction may be composed of: QS-21 2002AV1 (i.e., apiose isomer)

[1520]

[1521] and QS-21 2002A V2 (i.e. xylose isomers):

[1522]

[1523] - "QS-21 1988B component", which is identified as Figure 6 The triterpene glycosides are part of the B-isomer peak in the UPLC-UV / MS method and have an m / z of 1988 using negative mode electrospray mass spectrometry. Suitably, the QS-21 1988B fraction in the UPLC-UV / MS method described herein has a retention time of about 4.0 minutes and an m / z of 1988 using negative mode electrospray mass spectrometry. The QS-21 1988B fraction corresponds to the B-isomer xylose chemotype structures S6a (apiose isomer) and S4a (xylose isomer) characterized in Nyberg 2000 and Nyberg 2003. The QS-21 1988B fraction may be composed of: QS-21 1988B V1 (i.e., apiose isomer)

[1524]

[1525] and QS-21 1988B V2 (i.e. xylose isomers):

[1526]

[1527] - "QS-21 1856B component", which is identified as Figure 6 The QS-21 1856B fraction is a triterpene glycoside that is part of the B-isomer peak in the 1856B fraction and has an m / z of 1856 using negative mode electrospray mass spectrometry. The QS-21 1856B fraction corresponds to the B-isomer xylose chemotype structure S2a characterized in Nyberg 2000 and Nyberg 2003. The QS-21 1856B fraction may be composed of:

[1528]

[1529] - "QS-21 2002B Fraction" is a triterpene glycoside with m / z 2002, as determined by negative mode electrospray mass spectrometry. QS-21 2002B Fraction corresponds to the B-isomer rhamnose chemotype of structures S3a and S5a characterized by Nyberg 2000 and Nyberg 2003. QS-21 2002B Fraction may be composed of: QS-21 2002B Fraction V1 (i.e., apiose isomers)

[1530]

[1531] and QS-21 2002B V2 (i.e. xylose isomers):

[1532]

[1533] - Desarabinofuranosyl-QS-21 family components (i.e., triterpene glycosides that lack the arabinofuranosyl moiety of QS-21 family components). Desarabinofuranosyl-QS-21 family components are present in relatively low concentrations in extracts, meaning they have not been characterized in detail. Desarabinofuranosyl-QS-21 family components include:

[1534] -Dearabinofuranosyl-QS-21 1856A component (i.e. Figure 6 (The triterpene glycoside identified as part of the "lyophilized peak" in the UPLC-UV / MS method described herein) Suitably, the desarabinofuranosyl-QS-21 1856A component in the UPLC-UV / MS method described herein has a retention time of approximately 4.7 minutes, and the major component of the peak has an m / z of 1856 when using negative mode electrospray mass spectrometry. The QS-21 1856A component is believed to be identified as peak 96 in Kite 2004. The deduced structure has been identified by MS / MS as the major desarabinofuranosyl-QS-21 1856A component. The desarabinofuranosyl-QS-21 1856A component may be composed of the following: desarabinofuranosyl-QS-21 1856A V1 (i.e., apiose isomer)

[1535]

[1536] and / or desarabinofuranosyl-QS-21 1856A V2 (i.e., xylose isomer):

[1537] -Dearabinofuranosyl-QS-21 1712A component (i.e. Figure 6

[00145] Suitably, the desarabinofuranosyl-QS-21 1712A fraction in the UPLC-UV / MS method described herein has a retention time of about 4.7 minutes and an m / z of 1712 using negative mode electrospray mass spectrometry. The desarabinofuranosyl-QS-21 1712A fraction may consist of: desarabinofuranosyl-QS-21 1712A

[1538]

[1539] -Dearabinofuranosyl-QS-21 1870A fraction is a triterpene glycoside with m / z 1870, using negative mode electrospray mass spectrometry. Dearabinofuranosyl-QS-21 1870A fraction can be composed of the following: Dearabinofuranosyl-QS-21 1870A V1 (i.e., apiose isomer)

[1540]

[1541] and / or desarabinofuranosyl-QS-21 1870A V2 (i.e., xylose isomer):

[1542] -Dearabinofuranosyl-QS-21 1856B fraction, a triterpene glycoside, with m / z 1856, was analyzed by negative mode electrospray mass spectrometry. Dearabinofuranosyl-QS-21 1856B fraction may be composed of: Dearabinofuranosyl-QS-21 1856B V1 (i.e., apiose isomer)

[1543]

[1544] and / or desarabinofuranosyl-QS-21 1856B V2 (i.e., xylose isomer):

[1545] -Dearabinofuranosyl-QS-21 1712B component is a triterpene glycoside with m / z 1712, using negative mode electrospray mass spectrometry. Dearabinofuranosyl-QS-21 1712B component can be composed of the following: Dearabinofuranosyl-QS-211712B

[1546]

[1547] -Dearabinofuranosyl-QS-21 1870B fraction, a triterpene glycoside, with m / z 1870, was analyzed by negative mode electrospray mass spectrometry. The dearabinofuranosyl-QS-21 1870B fraction may be composed of the following: dearabinofuranosyl-QS-21 1870B V1 (i.e., apiose isomer)

[1548]

[1549] and / or desarabinofuranosyl-QS-21 1870B V2 (i.e., xylose isomer):

[1550]

[1551] - acetylated QS-21 family components (i.e., having an acetylated triterpene glycoside at the C3 position of the fucose of the QS-21 family component), for example:

[1552] - "Acetylated QS-21 2030A Fraction" is a triterpene glycoside with m / z 2030, as determined by negative mode electrospray mass spectrometry. Acetylated QS-21 2030A corresponds to the A-isomer xylose chemotype. Acetylated QS-21 2030A Fraction may be composed of: Acetylated QS-21 2030A V1 (i.e., apiose isomer)

[1553]

[1554] and / or acetylated QS-21 2030A V2 (i.e., xylose isomers):

[1555]

[1556] - "Acetylated QS-21 1898A fraction" is a triterpene glycoside with m / z 1898, as determined by negative mode electrospray mass spectrometry. Acetylated QS-21 1898A corresponds to the A-isomer xylose chemotype. The acetylated QS-21 1898A fraction may be composed of: acetylated QS-21 1898A

[1557]

[1558] - "Acetylated QS-21 2044A Fraction" is a triterpene glycoside with m / z 2044 as determined by negative mode electrospray mass spectrometry. Acetylated QS-21 2044A corresponds to the α isomer of the rhamnose chemotype. The acetylated QS-21 2044A Fraction may be composed of: Acetylated QS-21 2044A V1 (i.e., apiose isomer)

[1559]

[1560] and / or acetylated QS-21 2044A V2 (i.e., xylose isomers):

[1561]

[1562] The product saponins directly related to the engineered glucosidase polypeptides used in the method of the invention are those in which the glucose residue has been cut relative to the starting saponin. However, the engineered glucosidase polypeptides can be used in combination with other enzymes that can cut other sugar residues. The specific product saponins related to the engineered glucosidase polypeptides include:

[1563] - Deglucosyl-QS-17 family components;

[1564] - QS-21 family components; and

[1565] -Desarabinofuranosyl-QS-21 family components.

[1566] The product saponins directly associated with the engineered rhamnosidase polypeptides used in the methods of the present invention are those in which the rhamnose residue has been cleaved relative to the starting saponin. However, the engineered rhamnosidase polypeptides can be used in conjunction with additional enzymes capable of cleaving other sugar residues. Specific product saponins associated with the engineered rhamnosidase polypeptides include:

[1567] -QS-18 family components;

[1568] - QS-21 family components; and

[1569] -Acetylated QS-21 family member.

[1570] As used herein, the term "QS-18 family component" refers to the xylose chemical type QS-18 2150 component (A and B isomers, and celeryose and xylose isomers: QS-18 2150A V1, QS-18 2150A V2, QS-18 2150B V1, and QS-18 2150B V2), the xylose chemical type QS-18 2018 component (A and B isomers: QS-18 2018A and QS-18 2018B), and the rhamnose chemical type QS-18 2164 component (A and B isomers, and celeryose and xylose isomers: QS-18 2164A V1, QS-18 2164A V2, QS-18 2164B V1, and QS-18 2164B V2).

[1571] As used herein, the term "deglucosyl-QS-17 family components" refers to deglucosyl-QS-17 2134 components of the xylose chemical type (A and B isomers, and celeryose and xylose isomers: deglucosyl-QS-17 2134A V1, deglucosyl-QS-17 2134A V2, deglucosyl-QS-17 2134B V1, and deglucosyl-QS-17 2134B V2), deglucosyl-QS-17 2002 components of the xylose chemical type (A and B isomers: deglucosyl-QS-17 2002A and deglucosyl-QS-17 2002B), and deglucosyl-QS-17 2148 components of the rhamnose chemical type (A and B isomers, and celeryose and xylose isomers: deglucosyl-QS-17 2148A V1, anosyl-QS-17 2148A V2, anosyl-QS-17 2148B V1 and anosyl-QS-17 2148B V2).

[1572] As used herein, the term "QS-17 family component" refers to the xylose chemical type QS-17 2296 component (A and B isomers, and celery sugar and xylose isomers: QS-17 2296A V1, QS-17 2296A V2, QS-17 2296B V1, and QS-17 2296B V2), the xylose chemical type QS-17 2164 component (A and B isomers: QS-17 2164A and QS-17 2164B), and the rhamnose chemical type QS-17 2310 component (A and B isomers, and celery sugar and xylose isomers: QS-17 2310A V1, QS-17 2310A V2, QS-17 2310B V1, and QS-17 2310B V2).

[1573] As used herein, the term "QS-21 family component" refers to the xylose chemical type QS-21 1988 component (A and B isomers, and celeryose and xylose isomers: QS-21 1988A V1, QS-21 1988A V2, QS-21 1988B V1, and QS-21 1988B V2), the xylose chemical type QS-21 1856 component (A and B isomers: QS-21 1856A and QS-21 1856B), and the rhamnose chemical type QS-21 2002 component (A and B isomers, and celeryose and xylose isomers: QS-21 2002A V1, QS-212002A V2, QS-21 2002B V1, and QS-21 2002B V2).

[1574] As used herein, the term "dearabinofuranosyl-QS-18 family components" refers to xylose chemotype dearabinofuranosyl-QS-18 2018 components (A and B isomers, and apiose and xylose isomers: dearabinofuranosyl-QS-18 2018A V1, dearabinofuranosyl-QS-18 2018A V2, dearabinofuranosyl-QS-18 2018B V1, and dearabinofuranosyl-QS-18 2018B V2), xylose chemotype dearabinofuranosyl-QS-18 1886A and dearabinofuranosyl-QS-18 1886B), rhamnose chemotype dearabinofuranosyl-QS-18 2032 components (A and B isomers, and apiose and xylose isomers: desarabinofuranosyl-QS-18 2032AV1, desarabinofuranosyl-QS-18 2032A V2, desarabinofuranosyl-QS-18 2032B V1, and desarabinofuranosyl-QS-18 2032B V2).

[1575] The term "acetylated deglucosyl-QS-17 family component" as used herein refers to the xylose chemical type acetylated deglucosyl-QS-17 2176 component (celery sugar and xylose isomers: acetylated deglucosyl-QS-17 2176A V1 and acetylated deglucosyl-QS-17 2176A V2), the xylose chemical type acetylated deglucosyl-QS-17 2044A component, and the rhamnose chemical type acetylated deglucosyl-QS-17 2190 component (celery sugar and xylose isomers: acetylated deglucosyl-QS-17 2190AV1 and acetylated deglucosyl-QS-17 2190A V2).

[1576] As used herein, the term "dearabinofuranosyl-QS-21 family components" refers to components of the xylose chemical type dearabinofuranosyl-QS-21 1856 (A and B isomers, and apiose and xylose isomers: dearabinofuranosyl-QS-21 1856A V1, dearabinofuranosyl-QS-21 1856A V2, dearabinofuranosyl-QS-21 1856BV1, and dearabinofuranosyl-QS-21 1856B V2), components of the xylose chemical type dearabinofuranosyl-QS-21 1712 (A and B isomers: dearabinofuranosyl-QS-21 1712A and dearabinofuranosyl-QS-21 1712B), components of the rhamnose chemical type dearabinofuranosyl-QS-21 1870 components (A and B isomers, and apiose and xylose isomers: desarabinofuranosyl-QS-21 1870A V1, desarabinofuranosyl-QS-21 1870A V2, desarabinofuranosyl-QS-21 1870B V1, and desarabinofuranosyl-QS-21 1870B V2).

[1577] As used herein, the term "acetylated QS-21 family component" refers to the xylose chemical type acetylated QS-21 2030 component (celery sugar and xylose isomers: acetylated QS-21 2030A V1 and acetylated QS-21 2030A V2), the xylose chemical type acetylated QS-21 1898A component, and the rhamnose chemical type acetylated QS-21 2044 component (celery sugar and xylose isomers: acetylated QS-21 2044A V1 and acetylated QS-21 2044A V2).

[1578] Plant cell culture

[1579] The term "plant cell culture" should be understood as the in vitro culture of any plant tissue or any plant cell derived from any tissue of a saponin-producing plant, such as the plants described and mentioned above. Tissues or cells from plant cell culture are capable of synthesizing saponins. In the present invention, the term "capable of synthesizing saponins" refers to the ability of cells to synthesize and produce saponins. Depending on different factors, such as the source of the plant cell culture, the cell culture conditions, it may be necessary to trigger the synthesis of saponins and their production. In the present invention, the term "under conditions that result in the synthesis of saponins" refers to conditions that trigger and result in the effective synthesis of saponins and their production.

[1580] Plant cell cultures within the meaning of the present invention can be obtained and produced according to any known method. Lambert et al. (2011) provide a review of general in vitro cultures of saponin-producing plants. WO 94 / 10291 (incorporated herein by reference) discloses how to obtain in vitro cultures of plant cells from Quillaja saponaria that are capable of synthesizing and producing saponins.

[1581] Methods for culturing plant cells and general culture conditions for plant cells are known in the art. Conventional culture media known for plant cell culture, such as classic Murashige and Skoog (MS) medium, White medium or Linsmaler & Skoog medium can be used in the method of the present invention. These culture media typically contain at least one or more macronutrients, for example selected from NH4NO3, KNO3, CaCl2, MgSO4, KH2PO4, NH4Cl or KCl; at least one or more micronutrients, for example selected from KI, H3BO3, MnSO4, ZnSO4, Na2MoO4, CuSO4, CoCl2, DeSO4 or Na2EDTA; at least one or more vitamins, for example selected from inositol, niacin, pyridoxine-HCl or thiamine-HCl, for example in a total concentration of between 0.01 and 3 g / L, such as between 50 and 150 mg / L; at least one or more amino acids, such as glycine; at least one or more carbon sources, for example selected from sucrose, glucose or fructose; and at least one or more plant hormones, for example selected from one or more cytokinins, or one or more auxins, such as 2,4-D and / or NAA.

[1582] The plant cells used in the methods of the present invention can be derived from different tissues or organs of a given plant, such as primordium, leaves, stems, hairy roots, internodes, cambium, whether cultured in suspension in a liquid medium or cultured on a solid medium, such as callus. In one embodiment, the plant cells used in the methods of the present invention are derived from cambium, such as cambium meristematic cells (CMCs). In alternative embodiments, the plant cells are derived from hairy roots.

[1583] The plant cell used in the method of the present invention can be callus, for example, from the cambium of plant." callus " is generally defined as a dedifferentiated cell cluster cultured on a solidified culture medium. Callus can be produced from any plant tissue explant by any method known to those skilled in the art, such as the method described in WO 94 / 10291, US 2019 / 0134128 or WO15 / 082978. In short, the tissue explant from a small plant can be surface sterilized, for example, by thoroughly washing with clean water, using a disinfectant (such as hypochlorite), using a wetting agent (such as Tween or Triton), using an antibiotic and / or using an antifungal agent. Then, the surface-sterilized explant is usually placed on the surface of a solidified culture medium (such as agar) and incubated in a sterile environment until a large number of undifferentiated cells (usually requiring 2 to 12 weeks, for example 8 weeks) are grown near the plant source material. The callus can be gradually purified and further propagated by repeating similar solid medium cultures, i.e., for example, by rotating small pieces of callus formed in a previous solid medium culture onto fresh solid medium every four weeks. Callus culture conditions are well known in the art and include medium components (e.g., MS medium), pH range, carbon source, nitrogen source, macro- and micro-salts, vitamins, and growth regulators.

[1584] The callus formed and refined by successive culture on solid medium can be inoculated into liquid medium and cultivated, thereby obtaining suspension cell culture. The terms "suspension plant culture" and "plant cell suspension" are used interchangeably and refer to the in vitro culture of plant cells that are dispersed in liquid medium and grown in suspension. In one embodiment, the plant cell that is used for the inventive method is suspended and grown in liquid medium. In order for plant cells to be placed in suspension culture, for example, cells are taken out from callus and transferred to the sterile culture vessel that contains nutrient medium. It is understandable that the optimized culture medium for suspension cell lines may be different from the optimal culture medium for callus. Determining suitable and best culture medium is within the capabilities of those skilled in the art.

[1585] Those skilled in the art are also aware of the transition from callus to suspension cell culture (e.g., as described in WO 94 / 10291 or US 2019 / 0134128). Suspension culture conditions, including medium components, pH range, carbon source, nitrogen source, macro- and micro-salts, vitamins, and growth regulators, such as classic MS medium, are well known in the art. Once initiated and adapted to suspension growth, the suspension cells can be subcultured or propagated, for example, by dilution, for example, every four weeks.

[1586] The saponin synthesis of the plant cells used in the method of the present invention (i.e., plant cells capable of synthesizing saponins) can be optimized by inducing the cells with an inducer. Suitable inducers for the method of the present invention are monocarboxylic acid compound inducers, such as 5-chlorosalicylic acid, salicylic acid, acetylsalicylic acid, methyl esters, such as methyl jasmonate (MeJa), or chemically synthesized 2-HEJ. In one embodiment, the inducer is MeJa.

[1587] While the concentration of an inducer is often mentioned with reference to the volume of the culture medium, another way to define concentration is with reference to PCV%. The term "PCV" stands for packed cell volume and refers to the volume occupied by cells in the culture medium. It can be calculated as follows: PCV (%) = (cell pellet volume / sample volume) x 100. A suitable range for an inducer, such as MeJa, is 0.5 to 10 μM / PCV%.

[1588] The inducer can be added directly to the culture medium. Alternatively, the culture medium can be replaced with a medium containing the inducer.

[1589] The duration of induction can be adjusted depending on the plant cell in question. It is within the skill of the art to determine the optimal duration. Suitably, induction may occur over a period of between 1 and 14 days, more preferably between 2 and 10 days, and even more preferably between 3 and 8 days.

[1590] As an alternative or supplement to induction, the saponin synthesis of the plant cell used in the inventive method can be further optimized by the nitrogen of the depleted cell (for example, before induction). Nitrogen depletion (depletion) means reducing the level of any nitrogen source present in the culture medium. Suitably, for example, before induction, the cell culture medium of the cell is removed and replaced with a culture medium that does not contain a nitrogen source. The suitable range of the nitrogen depletion duration (before induction) is 1 to 9 days, suitably 2 to 7 days, more suitably 3 to 6 days.

[1591] Induction and / or nitrogen depletion are non-limiting examples of "conditions leading to saponin synthesis" in the sense of the present invention.

[1592] The saponin of the vegetable cell synthesis used in the inventive method can be secreted into the culture medium extracellularly and / or retained in the cell. Therefore, in the sense of the present invention, the initial saponin that is intended to carry out enzymatic modification or conversion can be included in the cell culture medium. In other words, the cell culture medium of the vegetable cell that can synthesize saponin can be used as starting material, and carries out enzymatic treatment. In some embodiments, optionally after depletion of nitrogen from culture medium and / or inducing cell with inducing agent as mentioned above, glycosidase is added to the culture medium. Alternatively, the initial saponin that is intended to carry out enzymatic modification or conversion in the sense of the present invention can be recovered from the intracellular content of vegetable cell. For example, the intracellular content of gained can be carried out enzymatic treatment by cracking vegetable cell (by any technology as known in the art).

[1593] In the sense of the present invention, the term "plant cell culture extract" is understood to mean any extract or material obtained from in vitro plant cell culture, including saponins, whether synthetic or recovered, including starting and / or product saponins. For example, a plant cell culture extract can be a plant cell, or a cracked plant cell. A plant cell culture extract can be crude, or partially or completely purified. Plant cell culture extracts can be obtained from Gypsophila, Soapwort (e.g., Vaccaria or Soapwort) or Quillaja (Bomford, 1992), for example, plant cell culture extracts obtained from Quillaja species. Specific plant cell culture extracts include those obtained from Quillaja angustifolia or Quillaja saponaria. In one embodiment, the plant cell culture extract is obtained from Quillaja angustifolia. In one embodiment, the plant cell culture extract is obtained from Quillaja angustifolia.

[1594] The term "recovery" is understood to mean the collection of saponins, e.g., saponins synthesized by plant cells or plant cell cultures. The saponins can be recovered from the culture medium. Alternatively, the saponins can be recovered from the plant cells, e.g., by extraction.

[1595] Any known extraction method is suitable (e.g., as described in WO 94 / 10291), using a non-aqueous polar solvent, extracting using an acidic or alkaline medium, or extracting by mechanically disrupting the plant cells (e.g., by ball milling, ultrasonic treatment). Alternatively, saponins can be extracted by freezing the cell pellet obtained after centrifugation of the cell culture (causing cell lysis). Suitably, the cell pellet is frozen at -20°C, more preferably at -70°C, for example for at least 24 hours.

[1596] Extraction can use water or lower alcohol (for example methyl alcohol or ethanol), comprise their mixture as solvent.In one embodiment, initial saponin obtains (for example, using comprises at least 80%v / v water, especially at least 90%v / v water, for example the solvent of at least 95%v / v water) by water extraction.In one embodiment, initial saponin obtains (for example, using comprises at least 80%v / v methyl alcohol, especially at least 90%v / v methyl alcohol, for example the solvent of at least 95%v / v methyl alcohol) by methanol extraction.In one embodiment, initial saponin obtains (for example, using comprises at least 80%v / v methyl alcohol, especially at least 90%v / v methyl alcohol, for example the solvent of at least 95%v / v methyl alcohol) by ethanol extraction.In one embodiment, initial saponin obtains (for example, using comprises at least 80%v / v ethanol, especially at least 90%v / v ethanol, for example the solvent of at least 95%v / v ethanol) by methanol / ethanol extraction. In one embodiment, the starting saponin is obtained by water / ethanol extraction (e.g., using a solvent comprising at least 20% v / v water, in particular at least 30% v / v water, for example at least 40% v / v water and at least 20% ethanol, in particular at least 30% v / v ethanol, for example at least 40% v / v ethanol). In one embodiment, the starting saponin is obtained by water / methanol extraction (e.g., using a solvent comprising at least 20% v / v water, in particular at least 30% v / v water, for example at least 40% v / v water and at least 20% methanol, in particular at least 30% v / v methanol, for example at least 40% v / v methanol).

[1597] Method of the present invention can be applicable to the starting saponin under various situations.The starting saponin can be the form of the minor component in the composition containing saponin (ignoring solvent, if any), for example the minor component in the plant cell culture extract.The starting saponin can be the form of the main component in the composition containing saponin, such as the main component in the plant cell culture extract.The starting saponin can be the form of the minor component in the processed (for example partially purified) plant cell culture extract.The starting saponin can be the form of the main component in the processed (for example partially purified) plant cell culture extract.In some embodiments, the starting saponin is substantially purified when enzymatically modified.In a further embodiment, the starting saponin is contained in crude material, for example crude plant cell culture extract.

[1598] Purification refers to the separation of a component from other components. Thus, partial purification means that a component is separated from other components to some extent. Substantial purification refers to the substantial separation of a component from other components, for example, wherein the component constitutes at least 50% w / w, especially at least 70%, especially at least 80%, for example at least 90% of the component content (purities are 50%, 70%, 80% and 90% respectively). In the case of plant cell culture extracts, partial purification refers to the separation of the starting saponin from other extracted components to some extent. In the case of plant cell culture extracts, substantial purification refers to the substantial separation of the starting saponin from other extracted components, for example, wherein the starting saponin constitutes at least 50% w / w, especially at least 70%, especially at least 80%, for example at least 90% of the extracted component content. Partial or substantial purification can be performed by various methods, including chromatography, filtration through a semipermeable membrane, treatment with a selective adsorbent such as polyvinylpolypyrrolidone (PVPP) and the like.

[1599] Although the starting saponin may be a specific chemical entity, in many cases involving obtaining a saponin by extraction, there may be a plurality of starting saponins that are enzymatically modified to provide their corresponding product saponins. As mentioned above, for individual saponins, the present invention can be applied to a plurality of starting saponins with necessary adjustments within a certain range. A plurality of starting saponins comprising related starting saponins can be enzymatically modified with equivalents simultaneously. A plurality of starting saponins comprising distinguishable starting saponins can be enzymatically modified differently simultaneously (in the presence of more than one enzyme) or continuously (processed sequentially with a single enzyme). A plurality of starting saponins can comprise related and distinguishable starting saponins.

[1600] The method of the present invention can be applied to the starting saponins in the following component forms:

[1601] - crude plant cell culture extracts, such as water and / or lower alcohol extracts, especially aqueous cell extracts;

[1602] - partially purified plant cell culture extracts, such as water and / or lower alcohol plant cell culture extracts, especially aqueous plant cell culture extracts;

[1603] - PVPP-treated plant cell culture extracts, such as PVPP-treated water and / or lower alcohol plant cell culture extracts, especially PVPP-treated plant cell culture aqueous extracts;

[1604] - Plant cell culture fraction equivalent to Quil A;

[1605] - a plant cell culture fraction equivalent to fraction A;

[1606] - a plant cell culture fraction corresponding to fraction B (see Nyberg 2003);

[1607] - a plant cell culture fraction equivalent to fraction C;

[1608] -QS-7 fraction;

[1609] - QS-17 fraction;

[1610] - QS-18 fraction; or

[1611] -QS-21 fraction.

[1612] The method of the present invention can be applied to the starting saponins in the following compositions:

[1613] -QS-7 family components and QS-18 family components;

[1614] -QS-7 family components and QS-17 family components;

[1615] - QS-17 family components and QS-18 family components;

[1616] - QS-7 family components, QS-17 family components and QS-18 family components.

[1617] Like other QS families, the QS-7 family members contain a variety of related structures, including xylose and rhamnose chemotypes, xylose and apiose isomers, and A and B isomers:

[1618]

[1619] Certain QS-7 family components may lack glucose or rhamnose attached to β-D-fucose (beta-D-fuc).

[1620] Enzymatic modification

[1621] The present invention provides enzymatic modifications of saponins obtained from in vitro cell plant cultures. The enzymatic modifications contemplated by the present invention include converting a starting saponin into a product saponin by removing one or more sugar residues from the starting saponin. Suitably, the enzymatic modifications contemplated in the present invention are converting a starting saponin into a product saponin by removing one or more sugar residues from the starting saponin.

[1622] In certain embodiments, the enzymatic modification comprises removing a single sugar residue from the starting saponin, i.e., removing the terminal sugar residue (an "exo-cutting" action). In other embodiments, the enzymatic conversion comprises removing multiple sugar residues from the starting saponin, i.e., cleaving at the sugar bond rather than the terminal position (an "endo-cutting" action), thereby removing multiple sugar residues (e.g., 2, 3, or 4 sugar residues) connected by the sugar bond.

[1623] Specific sugar residues that may be removed include (e.g., consist of):

[1624] - Glucose, especially terminal glucose, especially β-glucose, such as β-glucose from sapogenin glycosides, such as the β-D-glucose residues highlighted below:

[1625]

[1626] - Rhamnose, especially terminal rhamnose, especially α-rhamnose, such as α-rhamnose from sapogenin glycosides, such as the α-L-rhamnose residues highlighted below:

[1627]

[1628] in particular

[1629]

[1630] Specific monosaccharide enzymatic conversions of interest include:

[1631] -QS-18 family components to QS-21 family components, for example:

[1632] o QS-18 2150 components (i.e., QS-18 2150A and / or QS-18 2150B) to QS-21 1988 components, for example:

[1633] QS-18 2150A components to QS-21 1988A components, such as:

[1634] QS-18 2150A V1 to QS-21 1988A V1

[1635] QS-18 2150A V2 to QS-21 1988A V2

[1636] ■QS-18 2150B components to QS-21 1988B components, such as:

[1637] QS-18 2150B V1 to QS-21 1988B V1

[1638] QS-18 2150B V2 to QS-21 1998B V2

[1639] QS-18 2150V1 components (i.e., QS-18 2150A V1 and / or QS-18 2150B V1) to QS-21 1988V1 components, such as:

[1640] QS-18 2150A V1 to QS-21 1988A V1

[1641] QS-18 2150B V1 to QS-21 1988B V1

[1642] QS-18 2150V2 components (i.e., QS-18 2150A V2 and / or QS-18 2150B V2) to QS-21 1988V2 components, such as:

[1643] QS-18 2150A V2 to QS-21 1988A V2

[1644] QS-18 2150B V2 to QS-21 1988B V2

[1645] ○ QS-18 2018 components (i.e., QS-18 2018A and / or QS-18 2018B) to QS-21 1856 components, such as:

[1646] QS-18 2018A component to QS-21 1856A component

[1647] QS-18 2018B components to QS-21 1856B components

[1648] o QS-18 2164 components (i.e., QS-18 2164A and / or QS-18 2164B) to QS-21 2002 components, for example:

[1649] ■QS-18 2164A components to QS-21 2002A components, such as:

[1650] QS-18 2164A V1 to QS-21 2002A V1

[1651] QS-18 2164A V2 to QS-21 2002A V2

[1652] ■QS-18 2164B components to QS-21 2002B components, such as:

[1653] QS-18 2164B V1 to QS-21 2002B V1

[1654] QS-18 2164B V2 to QS-21 2002B V2

[1655] QS-18 2164V1 components (i.e., QS-18 2164A V1 and / or QS-18 2164B V1) to QS-21 2002V1 components, such as:

[1656] QS-18 2164A V1 to QS-21 2002A V1

[1657] QS-18 2164B V1 to QS-21 2002B V1

[1658] QS-18 2164V2 components (i.e., QS-18 2164A V2 and / or QS-18 2164B V2) to QS-21 2002V2 components, such as:

[1659] QS-18 2164A V2 to QS-21 2002A V2

[1660] QS-18 2164B V2 to QS-21 2002B V2

[1661] - Deglucosylation - QS-17 family components to QS-21 family components, such as:

[1662] o Deglucosyl-QS-17 2134 component (i.e., deglucosyl-QS-17 2134A and / or deglucosyl-QS-17 2134B) to QS-21 1988 component, such as:

[1663] ■ Deglucosylation - QS-17 2134A component to QS-21 1988A component, such as:

[1664] ● Deglucosylation - QS-17 2134A V1 to QS-21 1988A V1

[1665] ● Deglucosylation - QS-17 2134AV2 to QS-21 1988A V2

[1666] ■ Deglucosylation - QS-17 2134B component to QS-21 1988B component, for example:

[1667] ● Deglucosylation QS-17 2134B V1 to QS-21 1988B V1

[1668] ● Deglucosylation QS-17 2134B V2 to QS-21 1988B V2

[1669] ■ Deglucosyl-QS-17 2134 V1 components (i.e., Deglucosyl-QS-17 2134 A V1 and / or Deglucosyl-QS-17 2134B V1) to QS-21 1988 V1 components, for example:

[1670] ● Deglucosylation - QS-17 2134A V1 to QS-21 1988A V1

[1671] ● Deglucosylation QS-17 2134B V1 to QS-21 1988B V1

[1672] ■ Deglucosyl-QS-17 2134 V2 components (i.e., Deglucosyl-QS-17 2134 A V2 and / or Deglucosyl-QS-17 2134B V2) to QS-21 1988 V2 components, for example:

[1673] ● Deglucosylation - QS-17 2134A V2 to QS-21 1988A V2

[1674] ● Deglucosylation QS-17 2134B V2 to QS-21 1988B V2

[1675] o Deglucosyl-QS-17 2002 component (i.e., Deglucosyl-QS-17 2002A and / or Deglucosyl-QS-17 2002B) to QS-21 1856 component, such as:

[1676] ■ Deglucosylation QS-17 2002A component to QS-21 1856A component

[1677] ■ Deglucosylation QS-17 2002B component to QS-21 1856B component

[1678] o Deglucosyl-QS-17 2148 component (i.e., deglucosyl-QS-17 2148A and / or deglucosyl-QS-17 2148B) to QS-21 2002 component, such as:

[1679] ■ Deglucosylation - QS-17 2148A component to QS-21 2002A component, such as:

[1680] ● Deglucosylation - QS-17 2148A V1 to QS-21 2002A V1

[1681] ● Deglucosylation - QS-17 2148A V2 to QS-21 2002A V2

[1682] ■ Deglucosylation - QS-17 2148B component to QS-21 2002B component, for example:

[1683] ● Deglucosylation QS-17 2148B V1 to QS-21 2002B V1

[1684] ● Deglucosylation QS-17 2148B V2 to QS-21 2002B V2

[1685] ■ Deglucosyl-QS-17 2134 V1 components (i.e., Deglucosyl-QS-17 2134 A V1 and / or Deglucosyl-QS-17 2134B V1) to QS-21 1988 V1 components, for example:

[1686] ● Deglucosylation - QS-17 2148A V1 to QS-21 2002A V1

[1687] ● Deglucosylation QS-17 2148B V1 to QS-21 2002B V1

[1688] ■ Deglucosyl-QS-17 2134 V2 components (i.e., Deglucosyl-QS-17 2134 A V2 and / or Deglucosyl-QS-17 2134B V2) to QS-21 1988 V1 components, such as:

[1689] ● Deglucosylation - QS-17 2148A V2 to QS-21 2002A V2

[1690] ● Deglucosylation QS-17 2148B V2 to QS-21 2002B V2

[1691] -QS-17 family components to QS-18 family components, such as:

[1692] o QS-17 2296 components (i.e., QS-17 2296A and / or QS-17 2296B) to QS-18 2150 components, for example:

[1693] QS-17 2296A components to QS-18 2150A components, such as:

[1694] QS-17 2296A V1 to QS-18 2150A V1

[1695] QS-17 2296A V2 to QS-18 2150A V2

[1696] QS-17 2296B components to QS-18 2150B components, such as:

[1697] QS-17 2296B V1 to QS-18 2150B V1

[1698] QS-17 2296B V2 to QS-18 2150B V2

[1699] QS-17 2296V1 components (i.e., QS-17 2296A V1 and / or QS-17 2296B V1) to QS-18 2150V1 components, such as:

[1700] QS-17 2296A V1 to QS-18 2150A V1

[1701] QS-17 2296B V1 to QS-18 2150B V1

[1702] QS-17 2296V2 components (i.e., QS-17 2296A V2 and / or QS-17 2296B V1) to QS-18 2150V2 components, such as:

[1703] QS-17 2296A V2 to QS-18 2150A V2

[1704] QS-17 2296B V2 to QS-18 2150B V2

[1705] ○ QS-17 21 64 components (i.e., QS-17 2164A and / or QS-17 2164B) to QS-18 2018 components, for example:

[1706] QS-17 2164A component to QS-18 2018A component

[1707] QS-17 2164B component to QS-18 2018B component

[1708] o QS-17 2310 components (i.e., QS-17 2310A and / or QS-17 2310B) to QS-18 2164 components, for example:

[1709] QS-17 2310A components to QS-18 2164A components, such as:

[1710] QS-17 2310A V1 to QS-18 2164A V1

[1711] QS-17 2310A V2 to QS-18 2164A V2

[1712] QS-17 2310B components to QS-18 2164B components, such as:

[1713] QS-17 2310B V1 to QS-18 2164B V1

[1714] QS-17 2310 B V2 to QS-18 2164B V2

[1715] QS-17 2310V1 components (i.e., QS-17 2310A V1 and / or QS-17 2310B V1) to QS-18 2164V1 components, such as:

[1716] QS-17 2310A V1 to QS-18 2164A V1

[1717] QS-17 2310B V1 to QS-18 2164B V1

[1718] QS-17 2310V2 components (i.e., QS-17 2310A V2 and / or QS-17 2310B V2) to QS-18 2164V2 components, such as:

[1719] QS-17 2310A V2 to QS-18 2164A V2

[1720] QS-17 2310B V2 to QS-18 2164B V2

[1721] -QS-17 family components to deglucosyl-QS-17 family components, such as:

[1722] o QS-17 2296 fraction (i.e., QS-17 2296A and / or QS-17 2296B) to deglucosyl-QS-17 2134 fraction, such as:

[1723] QS-17 2296A fraction to deglucosyl-QS-17 2134A fraction, e.g.

[1724] QS-17 2296A V1 to deglucosyl-QS-17 2134A V1

[1725] QS-17 2296A V2 to deglucosyl-QS-17 2134A V2

[1726] QS-17 2296B fraction to deglucosyl-QS-17 2134B fraction, e.g.

[1727] QS-17 2296B V1 to deglucosyl-QS-17 2134B V1

[1728] QS-17 2296B V2 to deglucosyl-QS-17 2134B V2

[1729] QS-17 2296V1 fractions (i.e., QS-17 2296A V1 and / or QS-17 2296B V1) to deglucosyl-QS-17 2134V1 fractions, e.g.,

[1730] QS-17 2296A V1 to deglucosyl-QS-17 2134A V1

[1731] QS-17 2296B V1 to deglucosyl-QS-17 2134B V1

[1732] QS-17 2296V2 components (i.e., QS-17 2296A V2 and / or QS-17 2296B V1) to deglucosyl-QS-17 2134V2 components, such as:

[1733] QS-17 2296A V2 to deglucosyl-QS-17 2134A V2

[1734] QS-17 2296B V2 to deglucosyl-QS-17 2134B V2

[1735] o QS-17 2164 fraction (i.e., QS-17 2164A and / or QS-17 2164B) to deglucosyl-QS-17 2002 fraction, such as:

[1736] ■QS-17 2164A component to deglucosylation-QS-17 2002A

[1737] ■QS-17 2164B component to deglucosyl-QS-17 2002B

[1738] o QS-17 2310 fraction (i.e., QS-17 2310A and / or QS-17 2310B) to deglucosyl-QS-17 2148 fraction, such as:

[1739] QS-17 2310A fraction to deglucosyl-QS-17 2148A fraction, e.g.

[1740] QS-17 2310A V1 to deglucosyl-QS-17 2148A V1

[1741] QS-17 2310A V2 to deglucosyl-QS-17 2148A V2

[1742] ■QS-17 2310B component to QS-21, such as:

[1743] QS-17 2310B V1 to deglucosyl-QS-17 2148B V1

[1744] QS-17 2310B V2 to deglucosyl-QS-17 2148B V2

[1745] QS-17 2310V1 components (i.e., QS-17 2310A V1 and / or QS-17 2310B V1) to deglucosyl-QS-17 2148V1 components, such as:

[1746] QS-17 2310A V1 to deglucosyl-QS-17 2148A V1

[1747] QS-17 2310B V1 to deglucosyl-QS-17 2148B V1

[1748] QS-17 2310V2 components (i.e., QS-17 2310A V2 and / or QS-17 2310B V2) to deglucosyl-QS-17 2148V2 components, such as:

[1749] QS-17 2310 A V2 to deglucosyl-QS-17 2148A V2

[1750] QS-17 2310B V2 to deglucosyl-QS-17 2148B V2

[1751] Other enzymatic conversions of monosaccharides of interest include:

[1752] - Desarabinofuranosyl-QS-18 family components to Desarabinofuranosyl-QS-21 family components, such as:

[1753] o Desarabinofuranosyl-QS-18 2018 fraction (i.e., desarabinofuranosyl-QS-18 2018A and / or desarabinofuranosyl-QS-18 2018B) to desarabinofuranosyl-QS-21 1856 fraction, such as:

[1754] ■Desarabinofuranosyl-QS-18 2018A fraction to Desarabinofuranosyl-QS-21 1856A fraction, e.g.

[1755] Desarabinofuranosyl-QS-18 2018A V1 to Desarabinofuranosyl-QS-21 1856A V1

[1756] Desarabinofuranosyl-QS-18 2018A V2 to Desarabinofuranosyl-QS-21 1856A V2

[1757] ■Desarabinofuranosyl-QS-18 2018B fraction to Desarabinofuranosyl-QS-21 1856B fraction, e.g.

[1758] Desarabinofuranosyl-QS-18 2018B V1 to Desarabinofuranosyl-QS-21 1856B V1

[1759] Desarabinofuranosyl-QS-18 2018B V2 to Desarabinofuranosyl-QS-21 1856B V2

[1760] Desarabinofuranosyl-QS-18 2018V1 fraction (i.e., Desarabinofuranosyl-QS-18 2018A V1 and / or Desarabinofuranosyl-QS-18 2018B V1) to Desarabinofuranosyl-QS-21 1856V1 fraction, e.g.

[1761] Desarabinofuranosyl-QS-18 2018A V1 to Desarabinofuranosyl-QS-21 1856A V1

[1762] Desarabinofuranosyl-QS-18 2018B V1 to Desarabinofuranosyl-QS-21 1856B V1

[1763] Desarabinofuranosyl-QS-18 2018 V2 fractions (i.e., desarabinofuranosyl-QS-18 2018A V2 and / or desarabinofuranosyl-QS-18 2018B V2) to desarabinofuranosyl-QS-21 1856 V2 fractions, e.g.,

[1764] Desarabinofuranosyl-QS-18 2018A V2 to Desarabinofuranosyl-QS-21 1856A V2

[1765] Desarabinofuranosyl-QS-18 2018B V2 to Desarabinofuranosyl-QS-21 1856B V2

[1766] o Desarabinofuranosyl-QS-18 1886 fraction (i.e., desarabinofuranosyl-QS-18 1886A and / or desarabinofuranosyl-QS-18 1886B) to desarabinofuranosyl-QS-21 1712 fraction, such as:

[1767] ■Desarabinofuranosyl-QS-18 1886A component to Desarabinofuranosyl-QS-21 1712A

[1768] ■Desarabinofuranosyl-QS-18 1886B components to Desarabinofuranosyl-QS-21 1712B

[1769] o Desarabinofuranosyl-QS-18 2032 fraction (i.e., desarabinofuranosyl-QS-18 2032A and / or desarabinofuranosyl-QS-18 2032B) to desarabinofuranosyl-QS-21 1870 fraction, such as:

[1770] ■Desarabinofuranosyl-QS-18 2032A fraction to Desarabinofuranosyl-QS-21 1870A fraction, e.g.

[1771] Desarabinofuranosyl-QS-18 2032A V1 to Desarabinofuranosyl-QS-21 1870A V1

[1772] ●QS-18 2032A V2 to desarabinofuranosyl QS-21 1870A V2

[1773] ■ Dearabinofuranosyl-QS-18 2032B component to QS-21, such as:

[1774] Desarabinofuranosyl-QS-18 2032B V1 to Desarabinofuranosyl-QS-21 1870B V1

[1775] QS-17 desarabinofuranosyl-QS-18 2032B V2 to desarabinofuranosyl-QS-21 1870BV2

[1776] ■ Desarabinofuranosyl-QS-18 2032V1 fraction (i.e., desarabinofuranosyl-QS-18 2032A V1 and / or desarabinofuranosyl-QS-18 2032B V1) to desarabinofuranosyl-QS-21 1870V1 fraction, e.g.

[1777] Desarabinofuranosyl-QS-18 2032A V1 to Desarabinofuranosyl-QS-21 1870A V1

[1778] Desarabinofuranosyl-QS-18 2032B V1 to Desarabinofuranosyl-QS-21 1870B V1

[1779] ■ Desarabinofuranosyl-QS-18 2032V2 fraction (i.e., desarabinofuranosyl-QS-18 2032A V2 and / or desarabinofuranosyl-QS-18 2032B V2) to desarabinofuranosyl-QS-21 1870V2 fraction, e.g.

[1780] Desarabinofuranosyl-QS-18 2032A V2 to Desarabinofuranosyl-QS-21 1870A V2

[1781] Desarabinofuranosyl-QS-18 2032B V2 to Desarabinofuranosyl-QS-21 1870B V2

[1782] - Acetylated deglucosyl-QS-17 components to acetylated QS-21 family components, such as:

[1783] ○ Acetylated deglucosyl-QS-17 2176A to acetylated QS-21 2030A, such as:

[1784] ■Acetylated deglucosyl-QS-17 2176A V1 to acetylated QS-21 2030A V1

[1785] ■Acetylated deglucosyl-QS-17 2176A V2 to acetylated QS-21 2030A V2

[1786] ○ Acetylated deglucosyl QS-17 2044A to acetylated QS-21 1898A components

[1787] ○ Acetylated deglucosyl-QS-17 2190A to acetylated QS-21 2044A, such as:

[1788] ■Acetylated deglucosyl-QS-17 2190A V1 to acetylated QS-21 2044A V1

[1789] ■Acetylated deglucosyl-QS-17 2190A V2 to acetylated QS-21 2044 A V2.

[1790] Enzymatic conversion can be applied to single starting saponin or multiple starting saponin in parallel.Should be appreciated that, according to the composition of starting material and enzyme used, method can comprise above-mentioned conversion or be made up of above-mentioned conversion.In addition, although a method may be limited to using single enzyme to remove specific sugar residue or sugar residue group from (i) single starting saponin, (ii) starting saponin family or (iii) multiple starting saponin family; These methods can also use multiple enzymes to remove multiple sugar residues from (i) single starting saponin, (ii) starting saponin family or (iii) multiple starting saponin family.The method relating to multiple enzymes can be carried out in series (i.e. single enzyme is applied to saponin material at any time) or carried out in parallel (i.e. more than one enzyme is applied to saponin material at any time, for example two or three enzymes, especially two enzymes), or its combination.

[1791] Methods involving the removal of multiple sugar residues may include the removal of single (but different) sugar residues from multiple starting saponins and / or the removal of multiple sugar residues (e.g., 2, 3, or 4 residues, particularly 2 or 3, and especially 2 residues) from a particular starting saponin. The removal of multiple sugar residues from a particular starting saponin may involve the removal of a single residue and / or the removal of any combination of multiple residues in a single cleavage.

[1792] An exemplary method may comprise (e.g., consist of) removing glucose and rhamnose, particularly α-rhamnose residues and β-glucose residues, such as α-L-rhamnose residues and β-D-glucose residues, from sapogenin glycosides:

[1793]

[1794] Specific polysaccharide enzymatic conversions of interest include:

[1795] -QS-17 family components to QS-21 family components, such as:

[1796] o QS-17 2296 components (i.e., QS-17 2296A and / or QS-17 2296B) to QS-21 1988 components, for example:

[1797] QS-17 2296A components to QS-21 1988A components, such as:

[1798] QS-17 2296A V1 to QS-21 1988A V1

[1799] QS-17 2296A V2 to QS-21 1988A V2

[1800] QS-17 2296B components to QS-21 1988B components, such as:

[1801] QS-17 2296B V1 to QS-21 1988B V1

[1802] QS-17 2296B V2 to QS-21 1988B V2

[1803] QS-17 2296V1 components (i.e., QS-17 2296A V1 and / or QS-17 2296B V1) to QS-21 1988V1 components, such as:

[1804] QS-17 2296A V1 to QS-21 1988A V1

[1805] QS-17 2296B V1 to QS-21 1988B V1

[1806] QS-17 2296V2 components (i.e., QS-17 2296A V2 and / or QS-17 2296B V2) to QS-21 1988V2 components, such as:

[1807] QS-17 2296A V2 to QS-21 1988A V2

[1808] QS-17 2296B V2 to QS-21 1988B V2

[1809] o QS-17 2164 components (i.e., QS-17 2164A and / or QS-17 2164B) to QS-21 1856 components, for example:

[1810] ■QS-17 2164A component to QS-21 1856A component

[1811] ■QS-17 2164B component to QS-21 1856B component

[1812] o QS-17 2310 components (i.e., QS-17 2310A and / or QS-17 2310B) to QS-21 2002 components, for example:

[1813] ■QS-17 2310A components to QS-21 2002A components, such as:

[1814] QS-17 2310A V1 to QS-21 2002A V1

[1815] QS-17 2310A V2 to QS-21 2002A V2

[1816] ■QS-17 2310B components to QS-21 2002B components, such as:

[1817] QS-17 2310B V1 to QS-21 2002B V1

[1818] QS-17 2310B V2 to QS-21 2002B V2

[1819] QS-17 2310V1 components (i.e., QS-17 2310A V1 and / or QS-17 2310B V1) to QS-212002V1, e.g.:

[1820] QS-17 2310A V1 to QS-21 2002A V1

[1821] QS-17 2310B V1 to QS-21 2002B V1

[1822] QS-17 2310V2 components (i.e., QS-17 2310A V2 and / or QS-17 2310B V2) to QS-212002V2, e.g.:

[1823] QS-17 2310A V2 to QS-21 2002A V2

[1824] QS-17 2310B V2 to QS-21 2002B V2.

[1825] Plant cell culture extracts may contain a complex mixture of saponin components and therefore may undergo multiple transformations when multiple enzymes are present. For example, a starting mixture containing QS-17, QS-18, and deglucosyl-QS-17 components treated in parallel with appropriate β-glucosidase and α-rhamnosidase can undergo transformations including:

[1826] - QS-18 family components to QS-21 family components, in particular QS-18 2150 components to QS-21 1988 components;

[1827] - deglucosyl-QS-17 family components to QS-21 family components, especially deglucosyl-QS-17 2134 components to QS-21 1988 components;

[1828] -QS-17 family components to deglucosyl-QS-17 family components to QS-21 components, especially QS-17 2296 components to deglucosyl-QS-17 2134 components to QS-21 1988 components;

[1829] - QS-17 family components to QS-18 family components to QS-21 family components, especially QS-17 2296 component to QS-18 2150 component to QS-21 1988 component.

[1830] Enzyme selection

[1831] Extensive protein or DNA databases of natural and artificial glycosidases are available. Candidate enzymes can be selected and screened to assess their suitability for achieving a specific transformation under specific reaction conditions. The suitability of an enzyme depends on many factors, including:

[1832] -Target sugar (eg, glucose, rhamnose)

[1833] - Target sugar anomeric form (α or β);

[1834] -Target sugar enantiomer (D or L);

[1835] - Target sugar location (internal or external); and

[1836] - Target sugar environment (e.g., chemical / physical, affecting accessibility and reactivity).

[1837] Other factors that contribute to effective conversions include:

[1838] - conversion rate;

[1839] - Environmental sensitivity - including pH, temperature, substrate, product and contaminant concentration tolerance; and

[1840] - Specificity for the target sugar, including specificity with respect to other sugar residues, other anomers, other sugar residue positions, and between different residues of the same sugar anomer and positions within the substrate (if multiple such residues are present).

[1841] Those skilled in the art will appreciate that the level and type of specificity required for an enzyme will depend on the goals to be achieved and the general circumstances.

[1842] The conversion of QS-18 family components to QS-21 family components requires an enzyme exhibiting beta-exoglucosidase activity.

[1843] The conversion of QS-17 family components to deglucosyl-QS-17 family components requires an enzyme exhibiting beta-exoglucosidase activity.

[1844] The conversion of deglucosyl-QS-17 family components to QS-21 family components requires an enzyme exhibiting alpha exorhamnosidase activity.

[1845] The conversion of QS-17 family components to QS-18 family components requires an enzyme exhibiting alpha-exorhamnosidase activity.

[1846] It can be noted that many of the interesting Quillaja starting saponins contain only one glucose residue. Many of the interesting Quillaja starting saponins contain multiple rhamnose residues, so the selectivity for specific rhamnose residues is actually more important. For example, the conversion of deglucosyl-QS-17 family components to QS-21 family components or QS-17 family components to QS-18 family components requires the specificity of exorhamnosidase action relative to endo-rhamnosidase action. In addition, relative to other terminal rhamnose residues (e.g., in rhamnose chemical type components), the rhamnosidase specificity of α-O-rhamnosylation at the C2 position of the arabinofuranosyl moiety may also be desired. In certain embodiments, it may be necessary to remove the terminal rhamnose from the rhamnose chemical type components (alone or together with any α-O-rhamnosylation at the C2 position of the arabinofuranosyl moiety) to better promote their chromatographic separation from the xylose chemical type components.

[1847] In one embodiment, the enzymatic conversion is performed by a single enzyme. The single enzyme can be a glucosidase, especially a β exoglucosidase. The single enzyme glucosidase can be an engineered glucosidase polypeptide described in the present invention. Alternatively, the single enzyme is a rhamnosidase, especially an α exoglucosidase. The single enzyme rhamnosidase can be an engineered rhamnosidase polypeptide described in the present invention.

[1848] Preferred enzymes are those that are efficient in enzymatically converting the starting saponin to the desired product saponin while exhibiting limited or no undesirable conversion of other saponin components present.

[1849] In one embodiment, by more than a kind of enzyme, for example two or three enzymes, especially two kinds of enzymes carry out enzymatic transformation.The enzymatic modification (or conversion) that surpasses a kind of enzyme can comprise order / serial enzymatic modification.Perhaps, the enzymatic modification (or conversion) that surpasses a kind of enzyme can comprise simultaneously / parallel enzymatic modification.The enzymatic modification (or conversion) that is carried out by at least three kinds of enzymes can comprise the order / serial (modification carried out by a kind of enzyme) of any order and parallel / parallel (modification carried out by at least two other enzymes) enzymatic modification combination.When multiple enzymes are provided, these enzymes can be different proteins, or can be the form of one or more fusion proteins.

[1850] The enzyme of interest is a glucosidase, such as a β exoglucosidase. The glucosidase can be an engineered glucosidase polypeptide of the present invention. Another enzyme of interest is a rhamnosidase, such as an α exoglucosidase. The rhamnosidase can be an engineered rhamnosidase polypeptide of the present invention. Enzyme combinations of interest include, for example, those comprising a glucosidase and a rhamnosidase, particularly a β exoglucosidase and an α exoglucosidase. The enzymatic modification involving glucosidase and rhamnosidase, especially β exoglucosidase and α exoglucosidase, can be carried out as follows: sequentially with glucosidase (e.g. β exoglucosidase) followed by rhamnosidase (e.g. α exoglucosidase), sequentially with rhamnosidase (e.g. α exoglucosidase) followed by glucosidase (e.g. β exoglucosidase), or conveniently, simultaneously with glucosidase (e.g. β exoglucosidase) and rhamnosidase (e.g. α exoglucosidase). Specific enzyme combinations of interest are those comprising the engineered glucosidase of the present invention and the engineered rhamnosidase polypeptide of the present invention, for example, those consisting thereof.

[1851] The enzymes used are generally exogenous to the saponin material, ie are not enzymes naturally occurring in the saponin source obtained by extraction.

[1852] Enzyme can be natural, i.e. naturally occurring glycosidase, or can be non-naturally occurring glycosidase. In one embodiment, glucosidase is naturally occurring glucosidase (e.g., exoglucosidase, such as β exoglucosidase). In a second embodiment, glucosidase is non-naturally occurring glucosidase (e.g., exoglucosidase, such as β exoglucosidase). In one embodiment, rhamnosidase is naturally occurring rhamnosidase (e.g., exoglucosidase, such as α exoglucosidase). In a second embodiment, rhamnosidase is non-naturally occurring rhamnosidase (e.g., exoglucosidase, such as α exoglucosidase).

[1853] Enzymes can be modified ("engineered") relative to a reference enzyme. Point mutations introduced by engineering, whether singly or in combination, can provide benefits such as increased activity, increased specificity, increased stability, increased expression, and the like. Assays for demonstrating enzyme properties are well known to those skilled in the art. For example, activity can be quantified by the methods described in the Examples (see Examples 4-7) or similar methods.

[1854] Different enzymes may exhibit different sensitivities to environmental conditions, such as pH, temperature, substrate concentration, product concentration, solvent composition, presence of contaminants, etc. These parameters can be taken into account during the screening of candidate enzymes with the desired activity.

[1855] Candidate enzymes having beta-glucosidase activity include those in EC 3.2.1.21.

[1856] β-exoglucosidases of interest include those described in Table 7, in particular SEQ ID No. 262, 208, 63, 229, 250, 5, 101, 207, 169, 247, 302, 324, 319, 9, 240, 325 and 338, and functional variants thereof. Specific β-exoglucosidases of interest include SEQ ID No. 262, 208, 63, 229, 250, 5, 101, 207, 169, 247, 302, 324 and 319 and functional variants thereof, for example SEQ ID No. 262, 208, 63, 229, 250, 5, 101 and 207 and functional variants thereof.

[1857] Another group of beta exoglucosidases of interest include those described in Table 9, in particular SEQ ID Nos. 850, 879, 868, 826, 804, 888, 881, 891, 816, 827, 857, 853, 842, 814, 886, 885, 838, 829, 808, 828, 870, 873, 844, 882, 874, 825, 824, 823, 810, 894, 849, 803, 890, 841, 832, 830, 845, 871, 837, 883 and 809 and functional variants thereof. Specific beta exoglucosidases of interest include SEQ ID Nos. 850, 879, 868, 826, 804, 888, 881, 891, 816, 827, 857, 853, 842, 814, 886, 885, 838, 829, 808, 828, 870, 873, 844, 882, 874, 825, 824, 823, 810, 894, 849, 803, 890, and 841, and functional variants thereof, e.g., SEQ ID Nos. ID No. 850, 879, 868, 826, 804, 888, 881, 891, 816, 827, 857, 853, 842, 814, 886, 885, 838, 829, 808, 828, 870, 873, 844, 882, 874, 825, 824, 823, 810 and 894 and functional variants thereof.

[1858] SEQ ID No. 262 and its functional variants are particularly desirable β-exoglucosidases. In one embodiment, the β-exoglucosidase comprises, for example, consists of: (i) SEQ ID No. 262; or (ii) a functional variant thereof having at least 80% identity to SEQ ID No. 262, particularly at least 90%, in particular at least 95%, for example at least 96%, at least 97%, at least 98%, for example at least 99% identity; or (iii) a functional fragment of at least 100, particularly at least 200, in particular at least 300, for example at least 400, for example at least 500 consecutive amino acids of SEQ ID No. 262.

[1859] Candidate enzymes having alpha rhamnosidase activity include enzymes in EC 3.2.1.40.

[1860] Alpha exorhamnosidases of interest include SEQ ID Nos. 992, 1003, 1052, 1073, 1017, 1055, 1075, 1001, 1007, 1061, 1079, 1027, 1039, 1041, 989, 1053, 1018, 1066, 1082, 1076, 993, 1077, 1046, 1015, 1063, 1054, 1074, 1067, and 1033, and functional variants thereof. Specific alpha exorhamnosidases of interest include SEQ ID Nos. 992, 1003, 1052, 1073, 1017, 1055, 1075, 1001, 1007, 1061, 1079, 1027, 1039, 1041, 989, 1053, 1018, 1066, 1082, 1076, 993 and 1077 and functional variants thereof, for example SEQ ID Nos. 992, 1003, 1052, 1073, 1017, 1055, 1075, 1001, 1007, 1061, 1079, 1027, 1039, 1041 and 989 and functional variants thereof.

[1861] SEQ ID No. 1017 and its functional variants are particularly desirable exorhamnosidases. In one embodiment, the α-exorhamnosidase comprises, for example, consists of: (i) SEQ ID No. 1017; or (ii) a functional variant thereof having at least 80% identity to SEQ ID No. 1017, particularly at least 90%, especially at least 95%, such as at least 96%, at least 97%, at least 98%, such as at least 99% identity; or (iii) a functional fragment of at least 100, particularly at least 200, especially at least 300, such as at least 400, such as at least 500 consecutive amino acids of SEQ ID No. 1017.

[1862] Functional variants of interest in the present application include those comprising, for example consisting of: (i) a sequence that is at least 80% identical to the reference sequence, particularly at least 90%, in particular at least 95%, such as at least 96%, at least 97%, at least 98%, such as at least 99% identical; or (ii) a fragment of at least 100, particularly at least 200, especially at least 300, such as at least 400, such as at least 500 consecutive amino acids of the reference sequence.

[1863] Certain desired functional variants of interest include those comprising, for example consisting of, sequences having 1 to 20 additions, deletions and / or substitutions, in particular 1 to 15 additions, deletions and / or substitutions, in particular 1 to 10 additions, deletions and / or substitutions, such as 1 to 5 additions, deletions and / or substitutions relative to the reference sequence.

[1864] The degree of sequence identity can be determined by the homology alignment algorithm of Needleman and Wunsch, the ClustalW program or the BLASTP algorithm, using default settings. Algorithms using a global alignment (Needleman and Wunsch) are preferred.

[1865] "Percentage of sequence identity," "percent identity," and "percent identical" are used herein to refer to comparisons between polynucleotide sequences or polypeptide sequences and are determined by comparing two optimally aligned sequences within a comparison window, wherein the portion of the polynucleotide or polypeptide sequence within the comparison window may comprise additions or deletions (i.e., gaps) compared to the reference sequence for optimal alignment of the two sequences. Percentages are calculated by determining the number of positions at which the same nucleic acid base or amino acid residue occurs in the two sequences, or by aligning the nucleic acid base or amino acid residue with gaps to produce the number of matching positions, dividing the number of matching positions by the total number of positions in the comparison window, and multiplying the result by 100 to produce the percentage of sequence identity. Optimal alignment and determination of percentage of sequence identity are performed using the BLAST and BLAST 2.0 algorithms (see, e.g., Altschul, 1990; Altschul, 1997). Software for performing BLAST analysis is publicly available through the website of the National Center for Biotechnology Information. In brief, BLAST analysis involves first identifying high-scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence that match or satisfy some positive-valued threshold score T when aligned with a word of the same length in the database sequence. T is called the neighborhood word score threshold (Altschul, supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as long as the cumulative alignment score can be increased. For nucleotide sequences, the cumulative score is calculated using the parameters M (reward score for a pair of matching residues; always >0) and N (penalty score for mismatching residues; always <0). For amino acid sequences, a scoring matrix is ​​used to calculate the cumulative score. Extension of the word hits in each direction is stopped when: the cumulative alignment score drops by an amount X from its maximum achieved value; the cumulative score approaches zero or lower due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a word length (W) of 11, an expectation (E) of 10, M=5, N=-4, and a comparison of both chains. For amino acid sequences, the BLASTP program uses as defaults a word length (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff, 1989).

[1866] In terms of the percent identity of two sequences provided, many other algorithms are available, and their functions are similar ...

Claims

1. A method for preparing a product saponin, the method comprising the following steps: (i) providing a plant cell culture extract comprising saponin; and (ii) enzymatically converting starting saponins from said plant cell culture extract into product saponins.

2. A method for preparing a product saponin, the method comprising the following steps: (i) culturing a plant cell capable of synthesizing saponins under conditions leading to the synthesis of saponins; (ii) recovering saponins from plant cell cultures; as well as (iii) enzymatically converting the starting saponin from the recovered saponin into the product saponin.

3. A method for preparing a product saponin, the method comprising the following steps: (i) culturing a plant cell capable of synthesizing saponins under conditions leading to the synthesis of saponins; (ii) enzymatically converting a starting saponin from a synthesized saponin into said product saponin; and (iii) Recovery of saponins from plant cell cultures.

4. A method for increasing the amount of product saponin obtainable from plant cell culture, the method comprising the steps of: (i) culturing a plant cell capable of synthesizing saponins under conditions leading to the synthesis of saponins; (ii) recovering saponins from plant cell cultures; as well as (iii) enzymatically converting the starting saponin from the recovered saponin into the product saponin.

5. A method for increasing the amount of product saponin obtainable from plant cell culture, the method comprising the steps of: (i) culturing a plant cell capable of synthesizing saponins under conditions leading to the synthesis of saponins; (ii) enzymatically converting a starting saponin from a synthesized saponin into said product saponin; and (iii) Recovery of saponins from plant cell cultures.

6. A method for reducing the amount of starting saponins obtainable from a plant cell culture, the method comprising the steps of: (i) culturing a plant cell capable of synthesizing saponins under conditions leading to the synthesis of saponins; (ii) recovering saponin; as well as (iii) enzymatically converting the starting saponin from the recovered saponin to the product saponin.

7. A method for reducing the amount of starting saponins obtainable from a plant cell culture, the method comprising the steps of: (i) culturing a plant cell capable of synthesizing saponins under conditions leading to the synthesis of saponins; (ii) enzymatically converting a starting saponin from a synthesized saponin into a product saponin; and (iii) Recovery of saponins from plant cell cultures.

8. A method for producing saponins by plant cell culture, the method comprising the following steps: (i) culturing a plant cell capable of synthesizing saponins under conditions that result in the synthesis of saponins; and (ii) recovering saponin, wherein the yield of the product saponin is increased by enzymatically converting the starting saponin from the recovered saponin to the product saponin.

9. The method of any one of claims 1 to 8, wherein the saponin is sapogenin glycoside.

10. The method of any one of claims 1 to 9, wherein the plant cell culture extract or the plant cells are from Quillaja saponaria.

11. The method of claim 10, wherein the starting saponin is a QS-18 family component.

12. The method of claim 10, wherein the starting saponin is a desaccharyl-QS-17 family component.

13. The method of claim 10, wherein the starting saponin is a QS-17 family component.

14. The method of claim 10, wherein the starting saponin is a desarabinofuranosyl-QS-18 family member.

15. The method of claim 10, wherein the starting saponin is an acetylated desaccharyl-QS-17 family member.

16. The method of claim 11 or 12, wherein the product saponin is a QS-21 family component.

17. The method of claim 13, wherein the product saponin is a QS-18 family component.

18. The method of claim 13, wherein the product saponin is a deglucosylated QS-17 family component.

19. The method of claim 14, wherein the product saponin is a desarabinofuranosyl-QS-21 family member.

20. The method of claim 15, wherein the product saponin is an acetylated QS-21 family member.

21. The process of any one of claims 1 to 20, wherein a single starting saponin is converted to a single product saponin.

22. The method of any one of claims 1 to 20, wherein a plurality of starting saponins are converted into a plurality of product saponins.

23. The method of any one of claims 1 to 22, wherein the enzymatic conversion comprises removal of p-glucose residues by a glucosidase.

24. The method of claim 23, wherein the glucosidase comprises, for example, consists of, an amino acid sequence according to: SEQ ID No. 262, 208, 63, 229, 250, 5, 101, 207, 169, 247, 302, 324, 319, 9, 240, 325, 338, 850, 879, 868, 826, 804, 888, 881, 891, 816, 827, 857, 853, 842, 814, 886, 885, 838, 829, 808, 828, 870, 873, 844, 882, 874, 825, 824, 823, 810, 894, 849, 803, 890, 841, 832, 830, 845, 871, 837, 883 or 809, or a functional variant thereof.

25. The method of any one of claims 1 to 24, wherein the enzymatic conversion comprises removal of α-rhamnose residues by rhamnosidase.

26. The method of claim 25, wherein the rhamnosidase comprises, e.g., consists of, an amino acid sequence according to: SEQ ID No. 992, 1003, 1052, 1073, 1017, 1055, 1075, 1001, 1007, 1061, 1079, 1027, 1039, 1041, 989, 1053, 1018, 1066, 1082, 1076, 993, 1077, 1046, 1015, 1063, 1054, 1074, 1067 or 1033, or a functional variant thereof.

27. A saponin prepared by the method of any one of claims 1 to 26.

28. Use of a saponin prepared by the method of any one of claims 1 to 26 for preparing an adjuvant.

29. An adjuvant composition comprising the saponin of claim 27.

30. An immunogenic composition comprising the saponin according to claim 27 and an antigen or a polynucleotide encoding the antigen.

31. A kit comprising: (i) the saponin according to claim 27; and (ii) an antigen or a polynucleotide encoding an antigen.

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