A culture device for snow lotus stem cells and its application

Abstract

The invention relates to the technical field of stem cell culture, and discloses a culture device for snow lotus stem cells and an application thereof. The device comprises a horizontal support and a vertical support, wherein both sides between two adjacent horizontal supports are fixedly connected with vertical supports, and the horizontal support and the vertical support jointly form a main frame, a third display rack is arranged at the center of the top of the horizontal support, a driving component is arranged at both sides of the top of the horizontal support, a second display rack is arranged at both sides of the third display rack, a first display rack is arranged at one side of the driving component away from the third display rack, and culture dishes are arranged on the tops of the first display rack, the second display rack and the third display rack, and the driving component is started to drive the first display rack, the second display rack and the third display rack to rise in a step-by-step manner, so as to display the culture dishes in layers; so that the heights of the culture dishes in each column are staggered one by one, so that the culture state of the stem cells inside each culture dish can be visually observed outside the main frame.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell culture, and in particular to a culture device for snow lotus stem cells and application thereof. Background Art

[0002] Snow lotus stem cell culture refers to the use of biotechnology to isolate stem cells from snow lotus plants, and to amplify and differentiate them under specific culture conditions to obtain a large number of snow lotus stem cells or their derivatives. This technology not only helps to protect endangered snow lotus plants, but also provides precious raw materials for the pharmaceutical, cosmetics and other industries.

[0003] The existing snow lotus stem cell culture is to place a single snow lotus stem cell in a culture dish, then store it in batches in the laboratory, and observe the stem cell culture status regularly. However, during the batch storage process, a single-layer rack will hold multiple culture dishes. It is difficult to observe the culture dishes located in the center of the rack when observing from the outside. They need to be picked up one by one for observation. During the picking process, it is easy to accidentally touch other culture dishes, causing the culture dishes to tip over, thereby damaging the culture dishes and affecting the final culture effect.

[0004] To this end, the present invention provides a culture device for snow lotus stem cells and application thereof. Summary of the invention

[0005] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0006] The present invention provides a culture device for snow lotus stem cells and an application thereof. The device comprises a horizontal support and a vertical support, both sides between two adjacent horizontal supports are fixedly connected with vertical supports, the horizontal support and the vertical support jointly form a main frame, a third display rack is arranged at the center of the top of the horizontal support, both sides of the top of the horizontal support are arranged with a driving assembly, second display racks are arranged on both sides of the third display rack, a first display rack is arranged on one side of the driving assembly close to the third display rack, culture dishes are placed on the tops of the first display rack, the second display rack and the third display rack, and the driving assembly is started to drive the first display rack, the second display rack and the third display rack to rise in a step-by-step manner to display the culture dishes in layers.

[0007] By adopting the above technical solution, with the third display rack as the center, the height of each column of culture dishes decreases row by row, so that the height of each column of culture dishes is staggered one by one, so that the culture status of stem cells inside each culture dish can be visually observed outside the main frame.

[0008] Preferably, the driving assembly includes a rotating shaft, and the centers of the front and back sides of the rotating shaft are fixedly connected to the wire take-up drums, and a motor is fixedly installed on the top of the cross bracket, and the output shaft of the motor is fixedly connected to the wire take-up drum located on the back side.

[0009] Preferably, the first display rack includes a first frame body, the front and back sides of the first frame body are fixedly connected to the first fixed pulley, the outer side of the first fixed pulley is provided with a first pull rope, a positioning column is fixedly connected to the bottom of the front and back sides of the first frame body on one side close to the second display rack, one end of the first pull rope is fixedly connected to the outer side of the take-up drum, and the other end of the first pull rope is connected to the second display rack.

[0010] Preferably, the second display rack includes a second frame assembly, a first limiting strip is fixedly connected to a side of the second frame assembly close to the first display rack, a second fixed pulley is fixedly connected to the front and back sides of the second frame assembly, the bottoms of both sides of the front and back sides of the second frame assembly are fixedly connected to the positioning columns, a second pull rope is provided on the outer side of the second fixed pulley, and the other end of the first pull rope is fixedly connected to the outer side of the second pull rope close to the first display rack.

[0011] By adopting the above technical solution, the second frame components rise in a step-like manner, which is convenient for the operator to observe intuitively.

[0012] Preferably, the second rack assembly includes a rack body, a movable groove is provided in the middle of the rack body on a side away from the first rack, a movable hole is provided at the top of the movable groove, a storage groove is provided at the top of the movable hole, and limiting grooves are provided on the front and back sides of the rack body on a side away from the first rack.

[0013] Preferably, a first storage rack assembly is fixedly connected to a side of the second frame assembly close to the first display rack, the first storage rack assembly includes a storage tray, a support rod that fits with the clearance of the movable hole is fixedly connected to the top of the storage tray, a push plate that fits with the clearance of the storage slot is fixedly connected to the top of the support rod, and a side of the storage tray away from the first display rack is fixedly connected to the storage rack body.

[0014] By adopting the above technical solution, after the second frame assembly is raised, the first storage rack assembly completely exposes the culture dishes inside the first frame assembly or the second frame assembly at a lower position, making it easier to observe and take out the culture dishes.

[0015] Preferably, the third display rack includes a third frame body, the front and back sides of the third frame body are fixedly connected with second limit strips, the front side of the third frame body is provided with a movable square groove, and the inner side of the movable square groove is provided with a second storage rack.

[0016] Preferably, the front and back sides of the third frame are fixedly connected with mounting frames, the bottom of the mounting frame of the third frame is fixedly connected with an electric push rod, and the output shaft of the electric push rod is fixedly connected to the bottom of the second storage rack.

[0017] By adopting the above technical solution, when the third rack is located at the highest position, the electric push rod drives the second rack to rise to drag the culture dish out and expose it to the outside.

[0018] Preferably, the first frame has the same structure as the second frame assembly, the second storage rack has the same structure as the first storage rack assembly, and the shape and size of the limiting groove and the shape and size of the first limiting strip and the second limiting strip are clearance-matched.

[0019] On the other hand, the present application also provides a method for using a flexible anchor rod, comprising the following steps:

[0020] S1. Isolation of snow lotus stem cells: Select appropriate tissues from the snow lotus plant as culture materials, and separate stem cells from the tissues using gradient centrifugation;

[0021] S2. Place and culture the snow lotus stem cells: plant the separated snow lotus stem cells on a culture dish and add;

[0022] S3, observing the culture status of the snow lotus stem cells: the driving component is started to drive the first display rack, the second display rack, and the third display rack to rise in a step-by-step manner. During the rising process, the culture dishes are exposed to the outside with the cooperation of the first shelf assembly and the second shelf, and the culture status of the snow lotus stem cells in the culture dishes is displayed in layers;

[0023] S4. Complete the culture and remove the snow lotus stem cells: After observing that the cell morphology meets the standards, use the vacuum filtration method to obtain the cultured snow lotus stem cells and put them into subsequent processing technology.

[0024] The beneficial effects of the present invention are as follows: the culture device of snow lotus stem cells and its application described in the present invention are provided with a driving component, a first display rack, a second display rack, and a third display rack that cooperate with each other. When the driving component is started, the first display rack, the second display rack, and the third display rack are driven to rise in a step-by-step manner, and the culture dishes are displayed in layers, such as Figure 3 As shown, with the third rack as the center, the heights of the culture dishes decrease row by row, so that the heights of the culture dishes in each row are staggered one by one, so that the culture status of stem cells inside each culture dish can be visually observed from the outside of the main frame.

[0025] The snow lotus stem cell culture device and application thereof described in the present invention are characterized by the multiple second frame assemblies and the third frame rising in sequence. When the second frame assembly rises, it will drive the first shelf assembly to rise, so that the first shelf assembly rises along the inner side of the first frame or the second frame assembly in a low position, and the culture dish is raised. After the second frame assembly is raised, the first shelf assembly completely exposes the culture dish inside the first frame or the second frame assembly in a low position to the outside, which is convenient for observation and taking the culture dish. In the initial state, the first frame, the second frame assembly and the third frame protect the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a three-dimensional diagram of the overall structure of the present invention;

[0027] Figure 2 It is a schematic diagram of the structure of the driving component in the present invention;

[0028] Figure 3 It is a schematic diagram of the observation state of the first display rack, the second display rack and the third display rack in the present invention;

[0029] Figure 4 It is a schematic diagram of the cross-sectional structure of the first display rack, the second display rack and the third display rack in the present invention in the observation state;

[0030] Figure 5 It is a schematic diagram of one side structure of the second rack assembly and the first storage rack assembly in the present invention;

[0031] Figure 6 It is a structural schematic diagram of the other side of the second frame assembly and the first storage rack assembly structure in the present invention.

[0032] Description of reference numerals:

[0033] 1. First rack; 101. First frame; 102. First fixed pulley; 103. First pull rope; 2. Second rack; 201. Second frame assembly; 2011. Rack body; 2012. Storage slot; 2013. Limiting slot; 2014. Movable hole; 2015. Movable slot; 202. First rack assembly; 2021. Storage tray; 2022. Support rod; 2023. Push plate; 203. First limiting strip; 204. Second fixed pulley; 205. Second pull rope; 3. Third rack; 301. Third frame; 302. Second rack; 303. Electric push rod; 304. Second limiting strip; 305. Movable square slot; 4. Driving assembly; 401. Wire reel; 402. Rotating shaft; 403. Motor; 5. Horizontal bracket; 6. Vertical bracket; 7. Positioning column. DETAILED DESCRIPTION

[0034] The subject matter described herein will now be discussed with reference to example embodiments. It should be understood that the discussion of these embodiments is only to enable those skilled in the art to better understand and implement the subject matter described herein, and the functions and arrangements of the elements discussed may be changed without departing from the scope of protection of the contents of this specification. Each example may omit, replace or add various processes or components as needed. In addition, the features described relative to some examples may also be combined in other examples. Example

[0035] like Figures 1 to 6 As shown, a culture device for snow lotus stem cells and its application according to an embodiment of the present invention, please refer to Figure 1 , Figure 2 , Figure 3 , including a horizontal bracket 5 and a vertical bracket 6, both sides of two adjacent horizontal brackets 5 are fixedly connected with vertical brackets 6, the horizontal bracket 5 and the vertical bracket 6 jointly form a main frame, a third display rack 3 is arranged at the center of the top of the horizontal bracket 5, and second display racks 2 are arranged on both sides of the third display rack 3, a first display rack 1 is arranged on the side of the driving component 4 close to the third display rack 3, and a driving component 4 is arranged on the side of the first display rack 1 away from the third display rack 3, and culture dishes are placed on the top of the first display rack 1, the second display rack 2, and the third display rack 3. When the driving component 4 is started, it drives the first display rack 1, the second display rack 2, and the third display rack 3 to rise in a step-by-step manner to display the culture dishes in layers.

[0036] Specifically, when the driving component 4 is started, the first display rack 1, the second display rack 2, and the third display rack 3 are raised in a step-by-step manner to display the culture dishes in layers, as shown in FIG. Figure 3 As shown, with the third display rack 3 as the center, the heights of the culture dishes decrease row by row, so that the heights of the culture dishes in each row are staggered one by one, so that the culture status of stem cells inside each culture dish can be visually observed outside the main frame.

[0037] Please refer to Figure 1 , Figure 3 The driving assembly 4 includes a rotating shaft 402, and the centers of the front and back sides of the rotating shaft 402 are fixedly connected to the wire take-up drum 401. A motor 403 is fixedly installed on the top of the horizontal bracket 5, and the output shaft of the motor 403 is fixedly connected to the wire take-up drum 401 located on the back.

[0038] Specifically, the motor 403 is started so that the output shaft of the motor 403 drives the wire take-up drum 401 located at the back to rotate, and the wire take-up drum 401 located at the front is rotated synchronously through the rotating shaft 402 .

[0039] Please refer to Figure 3The first display rack 1 includes a first frame body 101, and the front and back sides of the first frame body 101 are fixedly connected to the first fixed pulley 102, and the outer side of the first fixed pulley 102 is provided with a first pull rope 103. A positioning column 7 is fixedly connected to the side of the bottom of the front and back sides of the first frame body 101 close to the second display rack 2, one end of the first pull rope 103 is fixedly connected to the outer side of the take-up drum 401, and the other end of the first pull rope 103 is connected to the second display rack 2.

[0040] Specifically, the initial state of this device is as follows Figure 1 , Figure 2 As shown, when the take-up drum 401 rotates, the first pull rope 103 is rolled up so that one end of the first pull rope 103 is wrapped around the outside of the take-up drum 401 to reduce the length of the first pull rope 103. The length of the first pull rope 103 is shortened to pull the second display rack 2 adjacent to the first display rack 1 upward, so that a height difference appears between the adjacent first display rack 1 and second display rack 2 for easy observation.

[0041] Please refer to Figure 3 , Figure 4 The second display rack 2 includes a second frame assembly 201, and a first limiting strip 203 is fixedly connected to one side of the second frame assembly 201 close to the first display rack 1, and a second fixed pulley 204 is fixedly connected to the front and back sides of the second frame assembly 201. The bottoms of both sides of the front and back sides of the second frame assembly 201 are fixedly connected to the positioning column 7, and a second pull rope 205 is arranged on the outer side of the second fixed pulley 204, and the other end of the first pull rope 103 is fixedly connected to the outer side of the second pull rope 205 close to the first display rack 1.

[0042] Specifically, Figure 4 As shown, one side is the first display rack 1, and the other side is the second pull rope 205 of the second display rack 2, one end of the second pull rope 205 is fixedly connected to the positioning column 7 located on the front and back of the first frame 101, and the other end of the second pull rope 205 is fixedly connected to the positioning column 7 located on the front and back of the second display rack 2 on the other side and close to the second pull rope 205; both sides are the second pull rope 205 of the second display rack 2, and the two ends of the second pull rope 205 are respectively fixedly connected to the positioning column 7 located on the front and back of the second frame assembly 201 on both sides and close to one side of the second pull rope 205.

[0043] Specifically, when the length of the first pull rope 103 is shortened due to one end being wound around the outside of the take-up drum 401, the other end of the first pull rope 103 pulls the second pull rope 205 near the first display rack 1 to rise, and the second pull rope 205 rises, thereby causing the second frame assembly 201 near the first display rack 1 to rise. Since the lengths of the first pull rope 103 and the second pull rope 205 are fixed, the second pull rope 205 set on the front and back sides of the second frame assembly 201 near the first display rack 1 moves along the second fixed pulley 204 during the rising process of the second frame assembly 201, so that the length of the side of the second pull rope 205 away from the first display rack 1 becomes shorter, thereby pulling the adjacent second display rack 2 to rise synchronously. Similarly, the subsequent multiple second frame assemblies 201 rise in a stepped manner in sequence, which is convenient for the operator to observe intuitively.

[0044] Please refer to Figure 4 , Figure 5 , Figure 6 The second rack assembly 201 includes a rack body 2011, a movable groove 2015 is provided in the middle of the rack body 2011 away from the first rack 1, a movable hole 2014 is provided at the top of the movable groove 2015, a storage groove 2012 is provided at the top of the movable hole 2014, and a limiting groove 2013 is provided on the front and back sides of the rack body 2011 away from the first rack 1.

[0045] Please refer to Figure 2 , Figure 4 , Figure 5 The second frame assembly 201 is fixedly connected to the first storage rack assembly 202 on one side close to the first storage rack 1. The first storage rack assembly 202 includes a storage tray 2021. The top of the storage tray 2021 is fixedly connected to a support rod 2022 that is clearance-matched with the movable hole 2014. The top of the support rod 2022 is fixedly connected to a push plate 2023 that is clearance-matched with the storage slot 2012. The storage tray 2021 is fixedly connected to the storage rack body 2011 on the side away from the first storage rack 1.

[0046] Specifically, Figure 2As shown, in the initial state, the first frame 101, the second frame assembly 201, and the third frame 301 are all at the same height. At this time, the first rack assembly 202 is at the bottom, and the culture dish is placed on the top of the push plate 2023. In the initial state, the culture dish is placed on the inner side of the storage groove 2012, and the storage groove 2012 protects it to ensure the safety of the storage state of the culture dish. When the driving assembly 4 is started, the multiple second frame assemblies 201 rise in sequence. When the second frame assembly 201 rises, it will drive the first rack assembly 202 to rise, so that the first rack assembly 202 rises along the inner side of the first frame 101 or the second frame assembly 201 in the lower position, and the height of the culture dish is raised. After the second frame assembly 201 is raised, the first rack assembly 202 completely exposes the culture dish inside the first frame 101 or the second frame assembly 201 in the lower position, which is convenient for observation and more convenient for taking out the culture dish.

[0047] Please refer to Figure 3 The third display rack 3 includes a third frame body 301, and the front and back sides of the third frame body 301 are fixedly connected with second limiting strips 304. The front of the third frame body 301 is provided with a movable square groove 305, and the inner side of the movable square groove 305 is provided with a second storage rack 302.

[0048] Please refer to Figure 3 The front and back sides of the third frame 301 are fixedly connected with mounting frames, the bottom of the mounting frame of the third frame 301 is fixedly connected with an electric push rod 303, and the output shaft of the electric push rod 303 is fixedly connected to the bottom of the second storage rack 302.

[0049] Specifically, a circular hole is provided at the top of the movable square groove 305 to match the clearance of the support rod 2022, and a circular groove is provided at the top of the circular hole of the movable square groove 305 to match the clearance of the push plate 2023. Figure 3 As shown, the length of the bottom of the second storage rack 302 is greater than the length of the bottom of the second rack assembly 201 , so that the front and back of the top of the second storage rack 302 move up and down along the movable square slot 305 driven by the output shaft of the electric push rod 303 .

[0050] Please refer to Figure 4 , Figure 5 The first frame 101 has the same structure as the second frame assembly 201, the second storage rack 302 has the same structure as the first storage rack assembly 202, and the shape and size of the limiting groove 2013 and the shape and size of the first limiting strip 203 and the second limiting strip 304 are all clearance-matched.

[0051] Specifically, when the motor 403 starts to drive the take-up reel 401 to rotate, the first frame 101, multiple second frame components 201, and the third frame 301 will rise in a step-like process. The first limit bar 203 and the second limit bar 304 will rise along the first frame 101 and the second frame component 201, and the limit groove 2013 plays a limiting role.

[0052] On the other hand, the present application also provides an application of the above-mentioned snow lotus stem cell culture device, comprising the following steps:

[0053] S1. Isolation of snow lotus stem cells: Select appropriate tissues from the snow lotus plant as culture materials, and separate stem cells from the tissues using gradient centrifugation;

[0054] S2. Place and culture the snow lotus stem cells: plant the separated snow lotus stem cells on a culture dish and add;

[0055] S3, observing the culture status of snow lotus stem cells: the driving component 4 is started to drive the first display rack 1, the second display rack 2, and the third display rack 3 to rise in a step-by-step manner. During the rising process, the culture dishes are exposed to the outside with the cooperation of the first rack component 202 and the second rack 302, and the culture status of the snow lotus stem cells in the culture dishes is displayed in layers;

[0056] S4. Complete the culture and remove the snow lotus stem cells: After observing that the cell morphology meets the standards, use the vacuum filtration method to obtain the cultured snow lotus stem cells and put them into subsequent processing technology.

[0057] Working principle:

[0058] The motor 403 is started so that the output shaft of the motor 403 drives the wire take-up drum 401 at the back to rotate, and the wire take-up drum 401 at the front rotates synchronously through the rotating shaft 402. At this time, the first pull rope 103 is rolled up so that one end of the first pull rope 103 is wound around the outside of the wire take-up drum 401 to reduce the length of the first pull rope 103. The second display rack 2 adjacent to the first display rack 1 is pulled up by shortening the length of the first pull rope 103, so that a height difference appears between the adjacent first display rack 1 and the second display rack 2. Since the second display rack 2 adjacent to the first display rack 1 is higher The first pull rope 103 and the second pull rope 205 are fixed in length, so that the second pull rope 205 arranged on the front and back of the second rack 2 close to the first rack 1 moves along the second fixed pulley 204 during the rising process of the second rack 2, so that the length of the second pull rope 205 on the side away from the first rack 1 becomes shorter, thereby pulling the adjacent second rack 2 to rise synchronously. Similarly, the subsequent multiple second rack assemblies 201 rise in a step-like manner in sequence until the third rack 301 is also lifted to the highest state, which is convenient for the operator to observe intuitively;

[0059] In the initial state, the first frame 101, the second frame assembly 201, and the third frame 301 are all at the same height. At this time, the first rack assembly 202 is at the bottom, and the culture dish is placed on the top of the push plate 2023. In the initial state, the culture dish is placed inside the storage groove 2012, and the storage groove 2012 protects it to ensure the safety of the storage state of the culture dish. When the driving assembly 4 is started, the multiple second frame assemblies 201 rise in sequence. When the second frame assembly 201 rises, it will drive the first rack assembly 202 to rise, so that the first rack assembly 202 rises along the inner side of the first frame 101 or the second frame assembly 201 in the lower position, and the height of the culture dish is raised. After the second frame assembly 201 is raised, the first rack assembly 202 completely exposes the culture dish inside the first frame 101 or the second frame assembly 201 in the lower position, which is convenient for observation and more convenient for taking out the culture dish.

[0060] An example of the present specific implementation mode is described above, but the present embodiment is not limited to the above-mentioned specific implementation mode, which is merely illustrative and not restrictive. A person skilled in the art may make many forms inspired by the present embodiment, all of which are protected by the present embodiment.

Claims

1. A culture device for snow lotus stem cells, characterized in that: The invention comprises a horizontal support (5) and a vertical support (6), both sides of two adjacent horizontal supports (5) are fixedly connected with vertical supports (6), the horizontal support (5) and the vertical support (6) together form a main frame, a third placing rack (3) is arranged at the center of the top of the horizontal support (5), both sides of the top of the horizontal support (5) are provided with a driving assembly (4), both sides of the third placing rack (3) are provided with a second placing rack (2), a first placing rack (1) is arranged on the side of the driving assembly (4) close to the third placing rack (3), the tops of the first placing rack (1), the second placing rack (2) and the third placing rack (3) are all provided with culture dishes, and the driving assembly (4) is started to drive the third placing rack (3) to move the culture dishes. The first display rack (1), the second display rack (2), and the third display rack (3) are arranged to rise in a step-like manner to display the culture dishes in layers; the driving assembly (4) comprises a rotating shaft (402), the center of the front and back sides of the rotating shaft (402) are fixedly connected to a take-up drum (401), the top of the horizontal bracket (5) is fixedly mounted with a motor (403), and the output shaft of the motor (403) is fixedly connected to the take-up drum (401) located at the back; the first display rack (1) comprises a first frame body (101), the front and back sides of the first frame body (101) are fixedly connected to a first fixed pulley (102), the outer side of the first fixed pulley (102) is provided with a first pull rope (103), and the first frame A positioning column (7) is fixedly connected to the bottom of the front and back sides of the body (101) near the second display rack (2); one end of the first pull rope (103) is fixedly connected to the outer side of the take-up drum (401), and the other end of the first pull rope (103) is connected to the second display rack (2); the second display rack (2) comprises a second frame body component (201); a first limit strip (203) is fixedly connected to the side of the second frame body component (201) near the first display rack (1); a second fixed pulley (204) is fixedly connected to the front and back sides of the second frame body component (201); the bottoms of both sides of the front and back sides of the second frame body component (201) are fixedly connected to the positioning column (7); A second pull rope (205) is arranged on the outside of the second fixed pulley (204), and the other end of the first pull rope (103) is fixedly connected to the outside of the second pull rope (205) close to the first display rack (1); the second rack body component (201) comprises a display rack body (2011), a movable groove (2015) is provided in the middle of the display rack body (2011) away from the first display rack (1), a movable hole (214) is provided at the top of the movable groove (2015), a storage groove (2012) is provided at the top of the movable hole (2014), and limiting grooves (2013) are provided on the front and back sides of the display rack body (2011) away from the first display rack (1);The second frame body component (201) is fixedly connected to a first storage rack component (202) on a side close to the first storage rack (1); the first storage rack component (202) comprises a storage tray (2021); a support rod (2022) is fixedly connected to the top of the storage tray (2021) and is clearance-matched with the movable hole (214); a push plate (2023) is fixedly connected to the top of the support rod (222) and is clearance-matched with the storage slot (2012); and a side of the storage tray (2021) away from the first storage rack (1) is fixedly connected to the storage rack body (2011). The third display rack (3) comprises a third frame body (301), the front and back sides of both sides of the third frame body (301) are fixedly connected to second limit bars (304), the front side of the third frame body (301) is provided with a movable square groove (305), and the inner side of the movable square groove (305) is provided with a second storage rack (302); the front and back sides of the third frame body (301) are fixedly connected to mounting racks, the bottom of the mounting rack of the third frame body (301) is fixedly connected to an electric push rod (303), and the output shaft of the electric push rod (303) is fixedly connected to the bottom of the second storage rack (302).

2. The culture device for snow lotus stem cells according to claim 1, characterized in that: The first frame (101) has the same structure as the second frame assembly (201), the second storage rack (302) has the same structure as the first storage rack assembly (202), and the shape and size of the limiting groove (2013) and the shape and size of the first limiting strip (203) and the second limiting strip (304) are all clearance-matched.

3. Application of a snow lotus stem cell culture device, according to the snow lotus stem cell culture device according to any one of claims 1-2, characterized in that: The following steps are involved: S1. Isolation of snow lotus stem cells: Select appropriate tissues from the snow lotus plant as culture materials, and separate stem cells from the tissues using gradient centrifugation; S2. Place and culture the snow lotus stem cells: plant the separated snow lotus stem cells on a culture dish and add; S3, observing the culture status of the snow lotus stem cells: the driving component (4) is started to drive the first display rack (1), the second display rack (2), and the third display rack (3) to rise in a step-by-step manner. During the rising process, the culture dishes are exposed to the outside with the cooperation of the first storage rack component (202) and the second storage rack (302), so that the culture status of the snow lotus stem cells in the culture dishes is displayed in layers; S4. Complete the culture and remove the snow lotus stem cells: After observing that the cell morphology meets the standards, use the vacuum filtration method to obtain the cultured snow lotus stem cells and put them into subsequent processing technology.

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