Method for extracting mixture from corn steep liquor
Through the process of multiple leaching and enzymatic decomposition, the problems of low efficiency and inconvenience in the existing technology are solved, and an efficient and convenient extraction process is achieved, and the quality and efficiency of corn slurry extracted substances are improved.
Patent Information
- Application Number
- CN202411336727.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-25
- Publication Date
- 2025-02-11
AI Technical Summary
In the prior art, there are problems of low efficiency and inconvenience in extracting substances from corn slurry.
A process including material selection, pretreatment, leaching, alkaline leaching, acid precipitation, filtration, neutralization, bleaching, enzymatic modification and filtration drying is adopted to thoroughly extract corn through multiple leaching and enzymatic decomposition. Various substances in the slurry.
It improves the efficiency of extracting substances, simplifies the process flow, reduces the consumption of preparations, protects the protein structure, and makes the extraction process more convenient.
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of biological fermentation extraction, and in particular relates to a method for extracting a mixture from corn steep liquor. Background Art
[0002] The corn steep liquor extract mixture includes a variety of substances such as protein, polysaccharides, vitamins, minerals, polypeptides and flavonoids, and can be widely used in feed, fermentation liquid, fermentation medium, etc. It has the characteristics of simple preparation and high nutritional value.
[0003] When corn steep liquor is used to extract the mixture, the steps mainly include crushing, screening, alkaline extraction, acid precipitation, atomization drying and the like. During the extraction process, the preparation consumption is large and the efficiency is low. In addition, during the extraction process, steps such as high temperature evaporation will destroy the protein structure, which is very inconvenient to use.
[0004] Application No.: 2021102732343, discloses a preparation process of corn syrup treated material, characterized in that the preparation process comprises the following steps: 1) preheating, 2) neutralization and sedimentation, 3) hydrolysis, 4) adding nutrient salts, 5) enzyme inactivation, and 6) filtration. The present invention can increase the economic benefits of corn processing enterprises and effectively alleviate the problem of relative shortage of protein resources.
[0005] In the above scheme, the extracted substances are relatively single, and although there are few steps, the process is relatively complicated and inconvenient to use. Summary of the invention
[0006] The purpose of the present invention is to solve the technical problems of low efficiency and inconvenience in extracting substances from corn steep liquor in the prior art, and to provide a method for extracting a mixture from corn steep liquor to solve the deficiencies of the prior art.
[0007] In order to achieve the above object, the present invention adopts the following technical solutions: A method for extracting a mixture from corn steep liquor comprises the steps of 1) selecting materials, 2) pretreatment, 3) leaching, 4) alkaline leaching, 5) acid precipitation, 6) filtering, neutralizing and bleaching, 7) enzymatic modification and 8) filtering and drying.
[0008] Furthermore, the process comprises the following steps: 1) Material selection: choose mature and healthy corn and pass it through a vibrating screen to remove sand and stones; 2) Pretreatment: drying at 50°C for 3 hours, crushing into 20-mesh powder particles, and filtering with a 20-40 mesh sieve to prepare corn flour; 3) Extraction: adding corn flour to 60% ethanol solution for extraction, with a solid-liquid weight ratio of 1:25-30, an extraction temperature of 55°C, and an extraction time of 5 hours; after the extraction is completed, solid-liquid filtration is performed to separate the solution a and the solid material a; Solution a is evaporated and concentrated until 1 / 3 of the original volume is reached or the ethanol is completely evaporated to obtain a concentrated solution; 4) alkaline leaching to prepare a sodium hydroxide solution with a Ph value of 9.5; For the first leaching, solid material a and sodium hydroxide solution are placed in a leaching tank for the first leaching, the solid-liquid weight ratio of solid material a to sodium hydroxide solution is 1:20, the leaching temperature is 50°C, and the leaching time is 4 hours; after the leaching is completed, solid-liquid filtration is performed to separate, and solution b and solid material b are obtained; Second leaching: solid material b is leached for the second time, the solid-liquid weight ratio of solid material b to sodium hydroxide solution is 1:10, the leaching temperature is 60° C., the leaching time is 2 hours, and solution c and slag are obtained; 5) Acid precipitation: Mix solution b and solution c at 50°C, add acetic acid to the mixture to neutralize it, stir it slightly, stop adding when the Ph value reaches 4, and then precipitate for 2 hours; 6) Filtration, neutralization, and bleaching: the concentrated solution in step 3 is mixed with the precipitated solution, and centrifuged at a speed of 6000 rpm for 5-10 minutes to obtain a mixed solution, and lime water and sodium hydroxide solution are added to the mixed solution to adjust the pH value to 7; 7) Enzymatic modification: the mixed solution after bleaching is enzymatically hydrolyzed, and composite protease is added to the solution. The weight ratio of the added composite protease to the mixed solution is 1:500. The Ph value of the mixed solution is 6.5, the temperature is 50°C, and it is stirred continuously. The enzymatic hydrolysis is carried out for 4 hours. The mixed solution after enzymatic hydrolysis is heated to 75°C and maintained for 3 minutes to inactivate the enzyme, and then cooled to 40°C; Add flavor protease to the cooled mixed solution, the weight ratio of flavor protease added to the mixed solution is 1:400, the Ph value of the mixed solution is 6, the temperature is 40°C, and it is stirred continuously for 8 hours of enzymolysis; 8) Filtration and drying: the mixed solution after enzymatic hydrolysis is centrifuged at a speed of 6000 rpm for 10 minutes. The filtered mixed solution is atomized and dried at a drying temperature of 60°C. The powder after drying is a mixed powder.
[0009] Furthermore, in the leaching and alkaline leaching, ultrasonic assisted leaching and leaching are used, and the frequency of the ultrasonic wave is adjusted to 60kHz and the power is 400w.
[0010] The invention performs one-time leaching, two-time leaching and two-time enzymolysis on corn, and the extraction is thorough, which effectively solves the technical problems of the prior art of numerous extraction procedures, inconvenient use and low efficiency. DETAILED DESCRIPTION
[0011] The technical solution of the present invention will be described clearly and completely below. In the description of the present invention, it is obvious that the embodiments described below are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0012] Embodiment 1, a method for extracting a mixture of corn steep liquor, comprising the steps of 1) selecting materials, 2) pretreatment, 3) leaching, 4) alkaline leaching, 5) acid precipitation, 6) filtration, neutralization, bleaching, 7) enzymatic modification, and 8) filtration and drying.
[0013] Furthermore, the process comprises the following steps: 1) Material selection: choose mature and healthy corn and pass it through a vibrating screen to remove sand and stones; 2) Pretreatment: drying at 50°C for 3 hours, crushing into 20-mesh powder particles, and filtering with a 20-40 mesh sieve to prepare corn flour; 3) Extraction: adding corn flour to 60% ethanol solution for extraction, with a solid-liquid weight ratio of 1:25-30, an extraction temperature of 55°C, and an extraction time of 5 hours; after the extraction is completed, solid-liquid filtration is performed to separate the solution a and the solid material a; Solution a is evaporated and concentrated until 1 / 3 of the original volume is reached or the ethanol is completely evaporated to obtain a concentrated solution; 4) alkaline leaching to prepare a sodium hydroxide solution with a Ph value of 9.5; For the first leaching, solid material a and sodium hydroxide solution are placed in a leaching tank for the first leaching, the solid-liquid weight ratio of solid material a to sodium hydroxide solution is 1:20, the leaching temperature is 50°C, and the leaching time is 4 hours; after the leaching is completed, solid-liquid filtration is performed to separate, and solution b and solid material b are obtained; Second leaching: solid material b is leached for the second time, the solid-liquid weight ratio of solid material b to sodium hydroxide solution is 1:10, the leaching temperature is 60° C., the leaching time is 2 hours, and solution c and slag are obtained; 5) Acid precipitation: Mix solution b and solution c at 50°C, add acetic acid to the mixture to neutralize it, stir it slightly, stop adding when the Ph value reaches 4, and then precipitate for 2 hours; 6) Filtration, neutralization, and bleaching: the concentrated solution in step 3 is mixed with the precipitated solution, and centrifuged at a speed of 6000 rpm for 5-10 minutes to obtain a mixed solution, and lime water and sodium hydroxide solution are added to the mixed solution to adjust the pH value to 7; 7) Enzymatic modification: the mixed solution after bleaching is enzymatically hydrolyzed, and composite protease is added to the solution. The weight ratio of the added composite protease to the mixed solution is 1:500. The Ph value of the mixed solution is 6.5, the temperature is 50°C, and it is stirred continuously. The enzymatic hydrolysis is carried out for 4 hours. The emulsion after enzymatic hydrolysis is heated to 75°C and kept for 3 minutes to inactivate the enzyme, and then cooled to 40°C; Add flavor protease to the cooled mixed solution, the weight ratio of flavor protease added to the mixed solution is 1:400, the Ph value of the mixed solution is 6, the temperature is 40°C, and it is stirred continuously for 8 hours of enzymolysis; 8) Filtration and drying: the mixed solution after enzymatic hydrolysis is centrifuged at a speed of 6000 rpm for 10 minutes. The filtered mixed solution is atomized and dried at a drying temperature of 60°C. The powder after drying is a mixed powder.
[0014] Embodiment 2, the process comprises the following steps: 1) Material selection: choose mature and healthy corn and pass it through a vibrating screen to remove sand and stones; 2) Pretreatment: drying at 50°C for 3 hours, crushing into 20-mesh powder particles, and filtering with a 20-40 mesh sieve to prepare corn flour; 3) Extraction: adding corn flour to 60% ethanol solution for leaching, with a solid-liquid weight ratio of 1:25-30, an immersion temperature of 55°C, an immersion time of 3 hours, a stirring rate of 120 rpm, an ultrasonic frequency of 60kHz, and a power of 400w; after the leaching is completed, solid-liquid filtration separation is performed to obtain solution a and solid material a; Solution a is evaporated and concentrated until 1 / 3 of the original volume is reached or the ethanol is completely evaporated to obtain a concentrated solution; 4) alkaline leaching to prepare a sodium hydroxide solution with a Ph value of 9.5; For the first leaching, solid material a and sodium hydroxide solution are placed in an leaching tank for the first leaching, the solid-liquid weight ratio of solid material a to sodium hydroxide solution is 1:20, the leaching temperature is 50°C, the leaching time is 3 hours, the stirring rate is 180 rpm, the frequency of the ultrasonic wave is adjusted to 60kHz, and the power is 400w; after the leaching is completed, solid-liquid filtration separation is performed to obtain solution b and solid material b; Second leaching: solid material b is leached for the second time, the solid-liquid weight ratio of solid material b to sodium hydroxide solution is 1:10, the leaching temperature is 60°C, the leaching time is 1.5 hours, the stirring rate is 180 rpm, the frequency of the ultrasonic wave is adjusted to 60kHz, and the power is 400w, to obtain solution c and slag; 5) Acid precipitation: Mix solution b and solution c at 50°C, add acetic acid to the mixture to neutralize it, stir it slightly, stop adding when the Ph value reaches 4, and then precipitate for 2 hours; 6) Filtration, neutralization, and bleaching: the concentrated solution in step 3 is mixed with the precipitated solution, and centrifuged at a speed of 6000 rpm for 5-10 minutes to obtain a mixed solution, and lime water and sodium hydroxide solution are added to the mixed solution to adjust the pH value to 7; 7) Enzymatic modification: the mixed solution after bleaching is enzymatically hydrolyzed, and composite protease is added to the solution. The weight ratio of the added composite protease to the mixed solution is 1:500. The Ph value of the mixed solution is 6.5, the temperature is 50°C, and it is stirred continuously. The enzymatic hydrolysis is carried out for 4 hours. The emulsion after enzymatic hydrolysis is heated to 75°C and kept for 3 minutes to inactivate the enzyme, and then cooled to 40°C; Add flavor protease to the cooled mixed solution, the weight ratio of flavor protease added to the mixed solution is 1:400, the Ph value of the mixed solution is 6, the temperature is 40°C, and it is stirred continuously for 8 hours of enzymolysis; 8) Filtration and drying: the mixed solution after enzymatic hydrolysis is centrifuged at a speed of 6000 rpm for 10 minutes. The filtered mixed solution is atomized and dried at a drying temperature of 60°C. The powder after drying is a mixed powder.
[0015] Through ultrasonic assisted leaching and extraction, the extraction rate is higher and the time is shorter.
[0016] Example 3, bleaching: the mixed solution is put into a decolorization tank, activated carbon is added for decolorization, the decolorization time is 50 minutes, and the decolorization temperature is 70°C; Decolorization with decolorization membrane: Decolorize the decolorized clear liquid in the decolorization membrane, control the membrane inlet pressure at 0.8 MPa and the membrane outlet pressure at 0.8 MPa to obtain a decolorized clear liquid.
[0017] Example 4: The composition of the mixture of Example 1 was measured, and the content of protein was 9%, carbohydrates was 63%, and the content of vitamins and minerals was 4%.
[0018] When the mixture is used as a fermentation liquid, it can provide the bacteria with the required sugars and nutrients.
[0019] The mixture can provide required sugars and nutrients when used as a feed supplement.
[0020] Although the above describes the specific implementation of the present invention in combination with the embodiments, it is not intended to limit the scope of protection of the present invention. Those skilled in the art should understand that it should be clear to those skilled in the art that these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection of the present invention.
Claims
1. A method for extracting a mixture from corn steep liquor, comprising the steps of 1) selecting materials, 2) pretreatment, 3) leaching, 4) alkaline leaching, 5) acid precipitation, 6) filtering, neutralizing and bleaching, 7) enzymatic modification and 8) filtering and drying.
2. The method for extracting a mixture from corn steep liquor according to claim 1, wherein: The process comprises the following steps: 1) Material selection: choose mature and healthy corn and pass it through a vibrating screen to remove sand and stones; 2) Pretreatment: drying at 50°C for 3 hours, crushing into 20-mesh powder particles, and filtering with a 20-40 mesh sieve to prepare corn flour; 3) Extraction: adding corn flour to 60% ethanol solution for extraction, with a solid-liquid weight ratio of 1:25-30, an extraction temperature of 55°C, and an extraction time of 5 hours; after the extraction is completed, solid-liquid filtration is performed to separate the solution a and the solid material a; Solution a is evaporated and concentrated until 1 / 3 of the original volume is reached or the ethanol is completely evaporated to obtain a concentrated solution; 4) alkaline leaching to prepare a sodium hydroxide solution with a Ph value of 9.5; For the first leaching, solid material a and sodium hydroxide solution are placed in a leaching tank for the first leaching, the solid-liquid weight ratio of solid material a to sodium hydroxide solution is 1:20, the leaching temperature is 50°C, and the leaching time is 4 hours; after the leaching is completed, solid-liquid filtration is performed to separate, and solution b and solid material b are obtained; Second leaching: solid material b is leached for the second time, the solid-liquid weight ratio of solid material b to sodium hydroxide solution is 1:10, the leaching temperature is 60° C., the leaching time is 2 hours, and solution c and slag are obtained; 5) Acid precipitation: Mix solution b and solution c at 50°C, add acetic acid to the mixture to neutralize it, stir it slightly, stop adding when the Ph value reaches 4, and then precipitate for 2 hours; 6) Filtration, neutralization, and bleaching: the concentrated solution in step 3 is mixed with the precipitated solution, and centrifuged at a speed of 6000 rpm for 5-10 minutes to obtain a mixed solution, and lime water and sodium hydroxide solution are added to the mixed solution to adjust the pH value to 7; 7) Enzymatic modification: the mixed solution after bleaching is enzymatically hydrolyzed, and composite protease is added to the solution. The weight ratio of the added composite protease to the mixed solution is 1:
500. The Ph value of the mixed solution is 6.5, the temperature is 50°C, and it is stirred continuously. The enzymatic hydrolysis is carried out for 4 hours. The mixed solution after enzymatic hydrolysis is heated to 75°C and maintained for 3 minutes to inactivate the enzyme, and then cooled to 40°C; Add flavor protease to the cooled mixed solution, the weight ratio of flavor protease added to the mixed solution is 1:400, the Ph value of the mixed solution is 6, the temperature is 40°C, and it is stirred continuously for 8 hours of enzymolysis; 8) Filtration and drying: the mixed solution after enzymatic hydrolysis is centrifuged at a speed of 6000 rpm for 10 minutes. The filtered mixed solution is atomized and dried at a drying temperature of 60°C. The powder after drying is a mixed powder.
3. The method for extracting a mixture from corn steep liquor according to claim 2, wherein: In the leaching and alkaline leaching, ultrasonic wave is used to assist the leaching and leaching, and the frequency of the ultrasonic wave is adjusted to 60 kHz and the power is 400 W.
4. The method for extracting a mixture from corn steep liquor according to claim 2, wherein: The bleaching: the mixed solution is put into a decolorization tank, activated carbon is added for decolorization, the decolorization time is 50 minutes, and the decolorization temperature is 70°C; Decolorization with decolorization membrane: Decolorize the decolorized clear liquid in the decolorization membrane, control the membrane inlet pressure at 0.8 MPa and the membrane outlet pressure at 0.8 MPa to obtain a decolorized clear liquid.