Cryopreservation device with integrated tracking device chamber

Abstract

The invention provides a cryopreservation device with an integrated tracking device chamber. The tracking device may be an RFID tag, which may be placed in a cryopreservation device as part of a procedure of glass curing of a biological sample. The cryopreservation device maintains its integrity and securely preserves an RFID tag in a chamber under environmental conditions when collecting a sample and under liquid nitrogen conditions when storing the sample. A cryopreservation device with an integrated tracking device chamber allows each individual biological sample to have its own tracking device for monitoring during cryopreservation.

Description

[0001] Cross-references

[0002] This application claims the benefit of U.S. Non-Provisional Application No. 18 / 136,419, filed on April 19, 2023, which is incorporated herein by reference in its entirety. Technical Field

[0003] The present invention relates generally to the field of devices for cryopreservation of biological specimens. More specifically, the present invention relates to devices capable of remote identification via RFID technology. Background Art

[0004] In life sciences, cryopreservation is performed for the purpose of stopping the biological activity of living cells for a prolonged period of time. Vitrification is one of the techniques used for cryopreservation.

[0005] Vitrification involves converting a solution containing a biological sample (i.e., oocyte or embryo) into a glassy amorphous solid without any crystalline structure, and then cooling extremely quickly. One of the main challenges of this method is to prevent the intracellular liquid in the oocyte or embryo from forming ice crystals. Therefore, the first step is to use a fluid containing a cryoprotectant called "vitrification medium" to dehydrate one or more cells as much as possible. The biological sample is then rapidly frozen by being immersed in a cryogenic fluid such as liquid nitrogen (LN2). Due to the appropriate combination of freezing speed and cryoprotectant concentration, the water in the cell will reach a solid, harmless, glassy (vitrification) state, rather than an orderly, destructive crystalline ice state. Vitrification can be described as a rapid increase in fluid viscosity, which captures water molecules along a random orientation. However, the vitrification medium may contain a relatively high level of cryoprotectant, which is toxic to cells except in a vitrified state. Therefore, the time that cells are exposed to the vitrification medium during dehydration and warming must be carefully controlled to avoid cell damage, and therefore it is expected that freezing will be performed as quickly as possible.

[0006] The CRYOLOCK cryopreservation device was developed as a versatile, simple and efficient vitrification device intended for preserving, freezing and storing oocytes or embryos in liquid nitrogen. It is a cryopreservation device that allows samples to be rapidly cooled and stored without direct contact with sterile liquid nitrogen (LN2). The CRYOLOCK cryopreservation device is described in U.S. Patent Application Publication No. US2016 / 0174545, published on June 23, 2016, which is incorporated herein by reference in its entirety.

[0007] The CRYOLOCK cryopreservation device uses a combination of semi-flexible plastics and functional design, including specific shapes and tight gaps, to hermetically seal samples within the cryopreservation device at room temperature and maintain that hermetic seal as the cryopreservation device and samples cool to liquid nitrogen temperatures.

[0008] Due to the expense of storing samples in liquid nitrogen, multiple samples are stored in a given cryostat. Since the entire cryostat is stored in the cryostat, the size of each cryostat and the packaging characteristics of such cryostat affect the amount of space required and, therefore, the cost of storage. Beyond simple space issues, quality management and auditing are required to ensure that the identification and storage conditions of each sample are tracked over time. If a cryostat needs to be removed from a cryostat during a quality audit, temperature fluctuations may affect the long-term viability of the sample.

[0009] There is a need to be able to identify each sample / device in a cryopreservation tank without removing the cryopreservation device from the cryopreservation tank. There is a need to maintain or reduce the size of cryopreservation devices that are self-identifying, particularly at the level of individual biological specimens. There is a need to maintain the ability to identify each sample / device throughout its lifecycle of collection, storage, retrieval, and use.

[0010] The addition of RFID tags in each cryogenic storage device has brought several challenges. First, the part of the device that keeps the biological sample must be sterilized, and it is usually sterilized using gamma rays, and gamma rays can damage the ability of the RFID tag to function. In addition, the addition of RFID tags must be suitable for the workflow during vitrification, which means that before collecting the biological sample, the RFID tag needs to be firmly engaged with a part of the cryogenic storage device, because the time between collecting the sample and being immersed in liquid nitrogen is necessarily shorter. The RFID tag needs to be firmly maintained by the cryogenic storage device under ambient conditions and liquid nitrogen temperature, and the cryogenic storage device cannot be damaged when such a large temperature fluctuation. Any size increase in the cryogenic storage device that can keep the RFID tag needs to be minimized. Ideally, the RFID tag can be fixed to the cryogenic storage device without the need for an adhesive that may be affected by low temperatures. Summary of the invention

[0011] According to an embodiment of the present invention, there is provided a cryopreservation device capable of holding a Radio Frequency Identification (RFID) tag. The cryopreservation device comprises: a) an elongated rod comprising an RFID section, an elongated body and a sample collection tip; and b) a cap comprising a hollow chamber, wherein the hollow chamber has a length sufficient to accommodate the sample collection tip. When the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip within the hollow chamber. The RFID section is remote from the sample collection tip, and the RFID section is capable of holding an RFID tag.

[0012] According to another embodiment of the present invention, a cryogenic storage device capable of holding an RFID tag is provided. The cryogenic storage device includes: a) an elongated rod including an RFID section, an elongated body, a frustoconical boss extending from a first end of the elongated body, and a sample collection tip extending from the frustoconical boss; and b) a cap including a hollow chamber, wherein the hollow chamber has a length sufficient to accommodate the sample collection tip and the frustoconical boss. When the cap is detachably attached to the elongated rod, the cap can enclose the sample collection tip and the frustoconical boss in the hollow chamber. The RFID section is away from the sample collection tip, and the RFID section can hold an RFID tag. The RFID section includes: a) an RFID chamber, the RFID chamber can hold an RFID tag; b) an RFID opening, the RFID tag can be placed into the RFID chamber through the RFID opening; and c) a locking mechanism, the locking mechanism abutting the periphery of the RFID opening.

[0013] According to another embodiment of the present invention, a cryogenic storage device capable of holding an RFID tag is provided. The cryogenic storage device comprises: a) an elongated rod comprising an RFID section, an elongated body, a truncated cone-shaped boss extending from a first end of the elongated body, and a sample collection tip extending from the truncated cone-shaped boss; and b) a cap comprising a hollow chamber, wherein the hollow chamber has a length sufficient to accommodate the sample collection tip and the truncated cone-shaped boss. When the cap is detachably attached to the elongated rod, the cap can enclose the sample collection tip in the hollow chamber. The RFID section is away from the sample collection tip, and the RFID section can hold an RFID tag. The RFID section comprises: a) an RFID chamber, the RFID chamber can hold the RFID tag; b) an RFID opening, the RFID tag can be placed into the RFID chamber through the RFID opening; and c) a locking mechanism, the locking mechanism abutting the periphery of the RFID opening. The elongated rod is made of a single piece of plastic. The RFID opening is located at the end of the elongated rod. The RFID chamber comprises a cylindrical chamber made of plastic with a prismatic shape as an outline, and the RFID tag is cylindrical. The prismatic shape has a first longitudinal slit and a second longitudinal slit, each slit starting from the RFID opening. The first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber. The first longitudinal slit traverses the RFID chamber from the second longitudinal slit. The locking mechanism comprises a platform at least partially surrounding the periphery of the RFID opening.

[0014] According to another embodiment of the present invention, a method for vitrifying a biological sample is provided. The method comprises: a) obtaining a cryogenic storage device of any one of the above embodiments; b) obtaining a liquid nitrogen-resistant RFID tag; c) placing the RFID tag in the RFID chamber; reading the RFID tag and associating the RFID tag with the biological sample; e) adding a vitrification mixture to dehydrate the biological sample; f) collecting the dehydrated biological sample on the elongated rod; g) sealing the dehydrated biological sample by fixing the cap to the elongated rod; and h) placing the cryogenic storage device in liquid nitrogen. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The present invention is illustrated and described herein with reference to the various drawings, in which like reference numerals represent like method steps and / or system components, respectively, and in which:

[0016] Figure 1ais a side view of the cryopreservation device of Comparative Example 1;

[0017] Figure 1b yes Figure 1a A cross-sectional view of the cryopreservation device along line AA;

[0018] Figure 1c There is no hat Figure 1a A side view of a cryopreservation device;

[0019] Figure 1d yes Figure 1c A cross-sectional view of the cryopreservation device along line AA;

[0020] Figure 2a is a side view of a cryopreservation device of an exemplary embodiment of Example 1;

[0021] Figure 2b yes Figure 2a a cross-sectional view of the illustrated embodiment along line AA;

[0022] Figure 2c yes Figure 2a A side view of the opposite side (rotated 180 degrees about the longitudinal axis) of the illustrated embodiment;

[0023] Figure 2d yes Figure 2a An isometric view of the RFID section of the illustrated embodiment;

[0024] Figure 3a is a side view of an exemplary embodiment of the cryopreservation device of Example 2;

[0025] Figure 3b yes Figure 3a A top view of the RFID segment and the end of the elongated rod of the illustrated embodiment;

[0026] Figure 3c yes Figure 3a An isometric view of the RFID segment and the end of the elongated rod of the illustrated embodiment;

[0027] Figure 4a is a side view of the cryopreservation device of Comparative Example 2;

[0028] Figure 4b yes Figure 4a A cross-sectional view of the cryopreservation device shown;

[0029] Figure 4c yes Figure 4a A detailed cross-sectional view of the RFID section of the cryopreservation device is shown;

[0030] Figure 5a is a side view of a cryopreservation device of an exemplary embodiment of Example 3;

[0031] Figure 5b yes Figure 5a a cross-sectional view of the illustrated embodiment;

[0032] Figure 5c yes Figure 5a a cross-sectional detail of an RFID section of the illustrated embodiment;

[0033] Figure 6a is a side view of another embodiment of a cryogenic storage device having an end loaded RFID segment and a plug for a locking mechanism;

[0034] Figure 6b yes Figure 6a A cross-sectional view of the RFID segment of the illustrated embodiment, an RFID tag that has not yet been loaded, and a plug removed from the RFID segment;

[0035] Figure 6c yes Figure 6a a cross-sectional view of an RFID section of the illustrated embodiment wherein an RFID tag is securely received within the RFID chamber;

[0036] Figure 7 is a cross-sectional view of another embodiment of a cryogenic storage device having an end-loaded RFID segment;

[0037] Figure 8a is a cross-sectional view of another embodiment of a cryogenic storage device having a removable RFID section;

[0038] Figure 8b is a cross-sectional view of a detachable RFID segment, Figure 8a The elongated body of the illustrated embodiment is in a disassembled state;

[0039] Figure 8c is a cross-sectional view of an alternative connection configuration of a cryogenic storage device and a corresponding removable RFID segment;

[0040] Figure 9a is a side view of another embodiment of a cryogenic storage device having a top-loading RFID section;

[0041] Figure 9b yes Figure 9a a cross-sectional view of an RFID section of the illustrated embodiment;

[0042] Fig.9c yes Figure 9a A detailed top view of the RFID section of the illustrated embodiment; and

[0043] Figure 9d yes Fig.9c Different cross-sectional views of an RFID segment of the illustrated embodiment. DETAILED DESCRIPTION

[0044] Embodiments of the present invention provide a cryogenic storage device capable of holding a radio frequency identification (RFID) tag. The cryogenic storage device includes: a) an elongated rod including an RFID segment, an elongated body, and a sample collection tip; and b) a cap including a hollow chamber, wherein the hollow chamber has a length sufficient to accommodate the sample collection tip. When the cap is removably attached to the elongated rod, the cap is capable of enclosing the sample collection tip within the hollow chamber. The RFID segment is distal to the sample collection tip, and the RFID segment is capable of holding an RFID tag.

[0045] The present invention may be more readily understood by reference to the following detailed description of the invention in conjunction with the accompanying drawings, which form a part of this disclosure. It should be understood that the present invention is not limited to the specific apparatus, methods, conditions or parameters described and / or illustrated herein, and that the terms used herein are only for the purpose of describing specific embodiments by way of example and are not intended to limit the claimed invention. Any and all patents and other publications identified in this specification are incorporated by reference as if fully set forth herein.

[0046] In addition, unless the context clearly dictates otherwise, as used in the specification, including the appended claims, the singular forms "a", "an", and "the" include plural, and references to a particular value include at least that particular value. Ranges may be expressed herein as from "about" or "approximately" one particular value and / or to "about" or "approximately" another particular value. When such a range is expressed, another embodiment includes from the one particular value and / or to the other particular value. Similarly, when a value is expressed as an approximation, by using the antecedent "about", it will be understood that the particular value forms another embodiment.

[0047] It should be understood that reference to one or more method steps does not preclude the presence of additional method steps before or after the recited combination of steps, or the presence of intervening method steps between those explicitly identified. Furthermore, code words for method steps or components are conventional means for identifying discrete activities or components, and unless otherwise indicated, the recited code words may be arranged in any order. A method step preceded by the word "optional" may appear, but this is not required.

[0048] As used herein, when the term "and / or" is used in a list of two or more items, it means that any one of the listed items may be taken alone, or any combination of the listed items or two or more items may be taken alone. For example, if a composition is described as containing compounds A, B "and / or" C, the composition may contain: A alone; B alone; C alone; a combination of A and B; a combination of A and C; a combination of B and C; or a combination of A, B, and C.

[0049] RFID tags are not particularly limited as long as they are compatible with liquid nitrogen. To be compatible with liquid nitrogen, RFID tags work as expected under ambient conditions and when immersed in liquid nitrogen (and therefore at the temperature of liquid nitrogen). RFID tags can work as expected when they can receive inquiries from an RFID reader and send the unique identification of the RFID tag back to the reader. In some aspects, an RFID tag is an object containing an integrated circuit, an antenna, and a substrate. In some aspects, the substrate comprises silicon and / or carbon fiber.

[0050] In some aspects, the cryopreservation device further comprises a truncated cone-shaped boss extending from the first end of the elongated body. In some aspects, the sample collection tip extends from the truncated cone-shaped boss. In some aspects, when the cap is removably attached to the elongated rod, the cap can enclose the sample collection tip within the hollow chamber. In some aspects, when the cap is removably attached to the elongated rod, the cap can hermetically enclose the sample collection tip within the hollow chamber.

[0051] In some aspects, the RFID section includes: a) an RFID cavity capable of holding an RFID tag; b) an RFID opening through which the RFID tag can be placed into the RFID cavity; and c) a locking mechanism adjacent a perimeter of the RFID opening.

[0052] In some aspects, the elongated rod is made of one piece of plastic. For example, when the elongated rod is injection molded in a mold that forms the RFID segment, the elongated body, the sample collection tip, and any other components of the elongated rod, the elongated rod is made of one piece of plastic. In another example, when the elongated rod is 3D printed, the elongated rod is made of one piece of plastic.

[0053] In some aspects, when the RFID tag is placed in the RFID chamber and the cryogenic storage device is placed in liquid nitrogen for 5 minutes, the RFID tag remains in the RFID chamber and the cryogenic storage device exhibits no visible cracks. In some aspects, when the RFID tag is placed in the RFID chamber and the cryogenic storage device is placed in liquid nitrogen for 5 minutes, the RFID tag remains in the RFID chamber and the cryogenic storage device exhibits no cracks when viewed at 10X magnification.

[0054] In some aspects, the shape of the RFID chamber corresponds to the shape of the RFID tag. In some aspects, the shape of the RFID tag is a cube, a cuboid, a cone, a cylinder, a sphere, a pyramid or a prism. In some aspects, the shape of the RFID tag is a cylindrical or cylindrical shape. In some aspects, the length range of the RFID tag is 4mm to 12mm long. In some aspects, the RFID has a diameter ranging from 0.5mm to 4mm, 0.5mm to 3.5mm, or 0.5mm to 3mm, or 0.5mm to 2.5mm, or 0.05mm to 2.2mm, or 0.5mm to 2mm, or 0.5mm to 1.8mm, or 0.5mm to 1.4mm, or 0.5mm to 1mm.

[0055] The RFID chamber having additional volume, width, and / or length relative to the RFID tag can allow the walls of the RFID chamber to shrink when immersed in liquid nitrogen while ensuring that the more rigid RFID tag does not rupture the walls of the RFID chamber. In some aspects, the volume of the RFID chamber is 1.01 times the volume of the RFID tag. Other non-limiting examples include the volume of the RFID chamber being at least 1.02 times, or 1.03 times, or 1.04 times, or 1.05 times, or 1.1 times the volume of the RFID tag. In some aspects, the width of the RFID chamber perpendicular to the longitudinal direction of the elongated rod is at least 1.01 times, or 1.02 times, or 1.03 times, or 1.04 times, or 1.05 times, or 1.1 times the corresponding width of the RFID tag. In some aspects, the length of the RFID chamber along the longitudinal direction of the elongated rod is at least 1.01 times, or 1.02 times, or 1.03 times, or 1.04 times, or 1.05 times, or 1.1 times the length of the RFID tag.

[0056] In some aspects, the RFID opening is located at the end of the elongated rod. In some aspects, the RFID chamber comprises a substantially cylindrical chamber made of plastic with a prism shape as the outline. In some aspects, the prism shape is a cuboid. In some aspects, the prism shape is hexagonal (prism). In some aspects, the RFID tag is substantially cylindrical, and wherein the prism shape has a first longitudinal slit and a second longitudinal slit, each slit starting from the RFID opening. In some aspects, the first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber. In some aspects, the first longitudinal slit is longer than the second longitudinal slit. In some aspects, the first longitudinal slit crosses the RFID chamber from the second longitudinal slit.

[0057] In some aspects, the RFID segment has a dimension of no greater than about 5 mm in each of the x-direction and the y-direction perpendicular to the longitudinal direction. In other examples, the RFID segment has a dimension of no greater than about 4.5 mm, or 4.0 mm, or 3.5 mm, or 3.1 mm, or 3.0 mm, or 2.8 mm, or 2.6 mm, or 2.4 mm in each of the x-direction and the y-direction perpendicular to the longitudinal direction.

[0058] In some aspects, the RFID opening is positioned along the longitudinal direction of the elongated rod. The RFID section includes a locking mechanism adjacent to the periphery of the RFID opening. In some aspects, the locking mechanism includes a platform that at least partially surrounds the periphery of the RFID opening. In some aspects, the length of the RFID opening is less than the length of the RFID tag, and the locking mechanism includes a portion of the periphery of the RFID section adjacent to the RFID opening and covering the RFID chamber. In some aspects, the RFID tag can be loaded into the RFID chamber at an angle to allow a portion of the RFID tag to enter the portion of the RFID chamber that exceeds the RFID opening, and then push the rest of the RFID tag through the RFID opening.

[0059] In some aspects, the length of the RFID opening is along the longitudinal axis of the elongated rod, wherein the length of the RFID opening is shorter than the length of the RFID chamber. In some aspects, the locking mechanism includes a circumferential flap that bends inward toward the RFID chamber. In some aspects, the locking mechanism also includes an obtrusion connected to the circumferential flap and protruding above the RFID opening. In some aspects, the RFID chamber has a dimension perpendicular to the longitudinal direction that is between 1.01% and 1.1% of the same dimension of the RFID tag.

[0060] Another embodiment of the present invention provides a cryopreservation device capable of holding an RFID tag. The cryopreservation device includes: a) an elongated rod including an RFID section, an elongated body, a truncated cone-shaped boss extending from a first end of the elongated body, and a sample collection tip extending from the truncated cone-shaped boss; and b) a cap including a hollow chamber, wherein the hollow chamber has a length sufficient to accommodate the sample collection tip and the truncated cone-shaped boss. When the cap is detachably attached to the elongated rod, the cap can enclose the sample collection tip and the truncated cone-shaped boss in the hollow chamber. The RFID section is away from the sample collection tip, and the RFID section can hold an RFID tag. The RFID section includes: a) an RFID chamber, the RFID chamber can hold an RFID tag; b) an RFID opening, the RFID tag can be placed into the RFID chamber through the RFID opening; and c) a locking mechanism, the locking mechanism abutting the periphery of the RFID opening.

[0061] It should be understood that the slender rod; RFID segment; RFID tag; RFID chamber; RFID opening; RFID locking mechanism; the manufacture of the slender rod; the shape and size of the RFID tag, RFID chamber and RFID opening; the relative sizes of various aspects of the RFID chamber relative to the RFID tag; the placement of the RFID opening; any longitudinal slits along the RFID chamber wall; and various aspects of the size of the RFID segment in each of the x-direction and the y-direction perpendicular to the longitudinal direction described above in this document also apply to the present embodiment.

[0062] In some aspects, the elongated rod is made of one piece of plastic. In some aspects, the RFID tag is cylindrical and has a length ranging from 6 mm to 12 mm long and a diameter ranging from 0.5 mm to 4 mm. Other non-limiting examples of RFID tag diameters are from 0.5 mm to 3.5 mm, or 0.5 mm to 3 mm, or 0.5 mm to 2.5 mm, or 0.05 mm to 2.2 mm, or 0.5 mm to 2 mm, or 0.5 mm to 1.8 mm.

[0063] In some aspects, the RFID opening is located at the end of the elongated rod. In some aspects, the RFID chamber comprises a substantially cylindrical chamber made of plastic with a prismatic shape as the outline. In some aspects, the prismatic shape is cuboid. In some aspects, the prismatic shape is hexagonal. In some aspects, the RFID tag is substantially cylindrical. In some aspects, the prismatic shape has a first longitudinal slit and a second longitudinal slit, each of the first longitudinal slit and the second longitudinal slit starting from the RFID opening. In some aspects, the locking mechanism comprises a platform at least partially surrounding the RFID opening.

[0064] In some aspects, the first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber. In some aspects, the first longitudinal slit is longer than the second longitudinal slit. In some aspects, the first longitudinal slit traverses the RFID chamber from the second longitudinal slit.

[0065] In some aspects, the RFID segment has a dimension of no greater than about 5 mm in each of the x-direction and the y-direction perpendicular to the longitudinal direction. In other examples, the RFID segment has a dimension of no greater than about 4.5 mm, or 4.0 mm, or 3.5 mm, or 3.1 mm, or 3.0 mm, or 2.8 mm, or 2.6 mm, or 2.4 mm in each of the x-direction and the y-direction perpendicular to the longitudinal direction.

[0066] Yet another embodiment of the present invention provides a cryopreservation device capable of holding an RFID tag. The cryopreservation device comprises: a) an elongated rod comprising an RFID section, an elongated body, a truncated cone-shaped boss extending from a first end of the elongated body, and a sample collection tip extending from the truncated cone-shaped boss; and b) a cap comprising a hollow chamber, wherein the hollow chamber has a length sufficient to accommodate the sample collection tip and the truncated cone-shaped boss. When the cap is detachably attached to the elongated rod, the cap can enclose the sample collection tip and the truncated cone-shaped boss in the hollow chamber. The RFID section is away from the sample collection tip, and the RFID section can hold an RFID tag. The RFID section comprises: a) an RFID chamber, the RFID chamber can hold the RFID tag; b) an RFID opening, the RFID tag can be placed into the RFID chamber through the RFID opening; and c) a locking mechanism, the locking mechanism abutting the periphery of the RFID opening. The elongated rod is made of a single piece of plastic. The RFID opening is located at the end of the elongated rod. The RFID chamber comprises a cylindrical chamber made of plastic with a prismatic shape as the outline, and the RFID tag is cylindrical. The prismatic shape has a first longitudinal slit and a second longitudinal slit, each starting from the RFID opening. The first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber. The first longitudinal slit traverses the RFID chamber from the second longitudinal slit. The locking mechanism comprises a platform at least partially surrounding the periphery of the RFID opening.

[0067] It should be understood that the slender rod; RFID segment; RFID tag; RFID chamber; RFID opening; RFID locking mechanism; the manufacture of the slender rod; the shape and size of the RFID tag, RFID chamber and RFID opening; the relative sizes of various aspects of the RFID chamber relative to the RFID tag; the placement of the RFID opening; any longitudinal slits along the RFID chamber wall; and various aspects of the size of the RFID segment in each of the x-direction and the y-direction perpendicular to the longitudinal direction described above in this document also apply to the present embodiment.

[0068] According to another embodiment of the present invention, a process for vitrifying biological samples is provided. The process includes the following steps: a) obtaining a cryogenic storage device of any one of the above embodiments; b) obtaining a liquid nitrogen-resistant RFID tag; c) placing the RFID tag in the RFID chamber; reading the RFID tag and associating the RFID tag with the biological sample; e) adding a vitrification mixture to dehydrate the biological sample; f) collecting the dehydrated biological sample on the elongated rod; sealing the dehydrated biological sample by fixing the cap to the elongated rod; and h) placing the cryogenic storage device in liquid nitrogen.

[0069] It should be understood that the slender rod; RFID segment; RFID tag; RFID chamber; RFID opening; RFID locking mechanism; the manufacture of the slender rod; the shape and size of the RFID tag, RFID chamber and RFID opening; the relative sizes of various aspects of the RFID chamber relative to the RFID tag; the placement of the RFID opening; any longitudinal slits along the RFID chamber wall; and various aspects of the size of the RFID segment in each of the x-direction and the y-direction perpendicular to the longitudinal direction described above in this document also apply to the present embodiment.

[0070] In some aspects, the RFID segment exhibits no visible cracks when placed in liquid nitrogen for 5 minutes.

[0071] The following is a non-limiting list of examples.

[0072] A1. A cryogenic storage device comprising

[0073] a) an elongated rod comprising an RFID section, an elongated body and a sample collection tip; and

[0074] b) a cap comprising a hollow chamber, wherein the hollow chamber has a length sufficient to accommodate the sample collection tip,

[0075] wherein, when the cap is removably attached to the elongated rod, the cap is capable of enclosing the sample collection tip within the hollow chamber, and

[0076] The RFID section is located away from the sample collection tip and is capable of holding an RFID tag.

[0077] A2. The cryogenic preservation device according to any one of embodiments A1, further comprising a truncated cone-shaped boss extending from the first end of the elongated body, wherein the sample collection tip extends from the truncated cone-shaped boss, and wherein when the cap is removably attached to the elongated rod, the cap is capable of enclosing the sample collection tip and the truncated cone-shaped boss within the hollow chamber.

[0078] A3. A low-temperature preservation device according to any one of embodiments A1 or A2, wherein the RFID section includes: a) an RFID chamber capable of holding an RFID tag; b) an RFID opening through which the RFID tag can be placed in the RFID chamber; and c) a locking mechanism adjacent to the periphery of the RFID opening.

[0079] A4. A cryogenic storage device according to any one of embodiments A1 to A3, wherein the elongated rod is made of a single piece of plastic; or wherein the elongated rod is produced by injection molding a polymer resin into a single mold; or wherein the elongated rod is produced by 3D printing.

[0080] A5. According to the cryogenic storage device described in any one of embodiments A1 to A4, when the RFID tag can be placed in the RFID chamber and the cryogenic storage device can be placed in liquid nitrogen for 5 minutes, the RFID tag remains in the RFID chamber and the cryogenic storage device shows no visible cracks, and / or the cryogenic storage device shows no cracks when observed at 10X magnification.

[0081] A6. A cryogenic storage device according to any one of embodiments A1 to A5, wherein the RFID tag is cylindrical and has a length ranging from 4 mm to 12 mm, and a diameter ranging from 0.5 mm to 4 mm, 0.5 mm to 3 mm, 0.5 mm to 2.5 mm, 0.5 mm to 2 mm, or 0.5 mm to 1.8 mm.

[0082] A7. A cryogenic storage device according to any one of embodiments A3 to A6, wherein the RFID opening is located at the end of the slender rod, wherein the RFID chamber comprises a substantially cylindrical chamber made of plastic with a prismatic shape as an outline, wherein the RFID tag is substantially cylindrical, and wherein the prismatic shape has a first longitudinal slit and a second longitudinal slit, each slit starting from the RFID opening.

[0083] A8. A low-temperature preservation device according to embodiment A7, wherein the first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber, and wherein the first longitudinal slit is longer than the second longitudinal slit.

[0084] A9. A cryogenic storage device according to any one of embodiments A7 or A8, wherein the first longitudinal slit crosses the RFID chamber from the second longitudinal slit.

[0085] A10. The cryogenic storage device of any one of embodiments A3 to A9, wherein the locking mechanism comprises a platform at least partially surrounding the periphery of the RFID opening.

[0086] A11. A low-temperature storage device according to any one of embodiments A1 to A10, wherein the dimension of the RFID segment in each of the x-direction and the y-direction perpendicular to the longitudinal direction is not greater than about 5 mm, or 4.5 mm, or 4.0 mm, or 3.5 mm, or 3.1 mm, or 3.0 mm, or 2.8 mm, or 2.6 mm.

[0087] A12. A cryogenic storage device according to any one of embodiments A1 to A11, wherein the dimension of the RFID segment in each of the x-direction and the y-direction perpendicular to the longitudinal direction is no greater than about 2.4 mm.

[0088] A13. A cryogenic storage device according to any one of embodiments A3 to A6, wherein the length of the RFID opening is along the longitudinal axis of the slender rod.

[0089] A14. A low-temperature storage device according to embodiment A13, wherein the length of the RFID opening is shorter than the length of the RFID chamber.

[0090] A15. A low-temperature storage device according to any one of embodiments A13 or A14, wherein the locking mechanism includes a circumferential flap that bends inwardly toward the RFID chamber.

[0091] A16. A low-temperature storage device according to embodiment A15, wherein the locking mechanism also includes a protrusion connected to the circumferential flap and protruding above the RFID opening.

[0092] A17. A cryogenic storage device according to any one of embodiments A13 to A17, wherein the RFID chamber has a dimension perpendicular to the longitudinal direction that is between 1.01% and 1.1% of the same dimension of the RFID tag.

[0093] A18. A cryogenic storage device according to any one of embodiments A13 to A17, wherein the dimension of the RFID segment in each of the x-direction and the y-direction perpendicular to the longitudinal direction is no greater than about 3 mm or no greater than about 2.4 mm

[0094] A19. The cryogenic storage device according to any one of embodiments A1 to A18, wherein the RFID tag is compatible with liquid nitrogen.

[0095] A20. A low-temperature storage device according to any one of embodiments A1 to A19, wherein the low-temperature storage device comprises at least one circumferential groove.

[0096] A21. A cryogenic preservation device according to any one of embodiments A1 to A20, wherein when the cap is detachably attached to the elongated rod, the cap is capable of hermetically sealing the sample collection tip within the hollow chamber.

[0097] A22. A cryogenic storage device according to any one of embodiments A3 to A6, A11, A12 or A17 to A21, wherein the RFID opening is located at the end of the slender rod and the locking mechanism includes a plug capable of blocking the RFID opening.

[0098] A23. A process for vitrifying a biological sample, the process comprising:

[0099] a) obtaining a cryopreservation device according to any one of embodiments A1 to A20;

[0100] b) obtaining the RFID tag;

[0101] c) placing the RFID tag into the RFID chamber;

[0102] d) reading the RFID tag and associating the RFID tag with the biological sample;

[0103] e) adding a glass solidification mixture to dehydrate the biological sample;

[0104] f) collecting the dehydrated biological sample on the sample collection tip;

[0105] g) enclosing the dehydrated biological specimen by detachably attaching the cap to the elongated rod;

[0106] h) placing the cryogenic storage device into liquid nitrogen.

[0107] Example

[0108] Comparative Example 1, Comparative Example 2, and Example 3 were performed using a slender rod of a cryopreservation device injection molded from polystyrene. Examples 1 and 2 were performed using a prototype of the slender rod of the cryopreservation device. The prototype was 3D printed by Digital ABS Plus from Statasys in Eden Prairie, Minnesota. The slender rod of each device / prototype was made of a single plastic integral piece. The slender rod in each example was designed with an RFID chamber to accommodate a transponder RFID microchip tag (RFID tag), which is cylindrical, has a diameter of 2 mm and a length of 12 mm, and can be obtained from Shenzhen Manruta Technology Co. Ltd. in Guangdong Province, China. The sample collection tip of each slender rod used for the comparative example and example can be sealed with a CRYOLOCK cap (Biotech, Inc., Alpharetta, GA).

[0109] Comparative Example 1

[0110] Design and injection molding Figure 1a to Figure 1d Cryogenic storage device shown. Figure 1a , Figure 1b , Figure 1c and Figure 1d , the cryopreservation device 10 includes an elongated stem 12 and a cap 14. The elongated stem includes an RFID section 16, a circumferential groove 18a, an elongated body 20, a truncated cone-shaped boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the boss 22. The cap 14 includes a circumferential groove 18b and an elongated hollow chamber 25 defined along a long axis of the cap 14 and sized to accommodate the sample collection tip 24 and the truncated cone-shaped boss 22. The circumferential grooves 18a, 18b disposed near the end of the cap 14 and the distal end of the elongated stem 12 are used to grip the cryopreservation device 10 with tweezers (not shown), thereby making it easier to manipulate the cryopreservation device 10 in a changing temperature environment. The RFID section 16 includes an RFID transition region 26, an RFID chamber 28, and an RFID termination region 30. The RFID termination region 30 is located farther from the elongated body 20 along the longitudinal axis than the RFID transition region 26, and the RFID cavity 28 is located between the RFID termination region 30 and the RFID transition region 26. The RFID cavity 28 is sized to hold an RFID tag (not shown). As such, the RFID cavity 28 is cylindrical in shape, and the opening 32 is circular. Figure 1c and Figure 1d 1 are side and cross-sectional views of the cryopreservation device 10 shown in FIG. 1 with the cap 14 removed so that the entire elongated rod 12 is shown.

[0111] The RFID tag was manually inserted into the RFID chamber 28 through the opening 32 under ambient conditions. The RFID tag was securely held in the RFID chamber 28 and the chip could not be removed from the RFID chamber 28 by shaking the elongated rod 12. The elongated rod 12 was immersed in liquid nitrogen for approximately 2 minutes. The elongated rod 12 was inspected when removed from the liquid nitrogen. The RFID chamber 28 had several cracks visible to the naked eye.

[0112] Example 1

[0113] Design as Figure 2a to Figure 2d The elongated rod 12 for a cryopreservation device is shown and a prototype is produced by 3D printing. Figure 2a , Figure 2b , Figure 2c and Figure 2d , the elongated rod 12 includes an RFID section 16, an elongated body 20, a truncated cone-shaped boss 22 extending from a first end of the elongated body, and a sample collection tip 24 extending from a narrow end of the truncated cone-shaped boss 22. The RFID section 16 includes a tapered RFID transition region 26, an RFID chamber 28, and an RFID terminal region 30. The RFID terminal region 30 is located farther away from the elongated body 20 along the longitudinal axis than the RFID transition region 26, and the RFID chamber 28 is located between the RFID terminal region 30 and the tapered RFID transition region 26. Because the RFID chamber 28 has a larger cross-section than the elongated body 20, the RFID transition region 26 is tapered or gradually becomes thinner. The size of the RFID chamber 28 is designed to hold an RFID tag (not shown).

[0114] The RFID tag is manually inserted into the RFID chamber 28 through the opening 32, which is sized to allow the RFID tag to pass through the opening 32 and into the RFID chamber 28. A locking mechanism 34 defines the opening 32. Figure 2a and Figure 2d The locking mechanism 34 shown ensures that the RFID tag (not shown) remains inside the RFID chamber 28 throughout the handling and storage of the elongated rod 12. The RFID tag is inserted at an angle that allows a portion of the RFID tag to enter the portion of the RFID chamber 28 beyond the RFID opening 32 and adjacent to the RFID termination area 30. Then as the RFID tag is pushed into the RFID chamber 28, once the RFID tag is fully inserted into the RFID chamber 28, the flaps 36a and 36b bend downward and rest on top of the RFID tag. Figure 2cAs shown, openings 37a and 37b are longitudinally spaced along the outer edge of the RFID chamber 28, and opening 32 is placed on opposite sides of the elongated rod 12 perpendicular to the longitudinal direction. The RFID tag is firmly held in the RFID chamber 28, and the chip cannot be removed from the RFID chamber 28 by shaking the elongated rod 12. The elongated rod 12 was immersed in liquid nitrogen. The RFID segment 16 was inserted into the liquid nitrogen for about 2 minutes and then returned to ambient conditions, and the RFID segment 16 showed no visible signs of cracking. The RFID segment 16 was observed at 10X magnification, and still no signs of cracking were observed.

[0115] Example 2

[0116] Design as Figure 3a The slender rod 12 for the cryopreservation device shown in FIG. 3 d is provided, and a prototype is 3D printed. The slender rod 12 includes a groove 18a, an RFID section 16, an elongated body 20, a truncated cone-shaped boss 22 extending from the first end of the elongated body 20, and a sample collection tip 24 extending from the narrow end of the truncated cone-shaped boss 22. The RFID section 16 includes a tapered RFID transition zone 26, an RFID chamber 28, and an RFID terminal zone 30. The RFID terminal zone 30 is positioned farther away from the elongated body 20 along the longitudinal axis than the RFID transition zone 26, and the RFID chamber 28 is located between the RFID terminal zone 30 and the RFID transition zone 26. The size of the RFID chamber 28 is designed to hold an RFID tag 38. The tapered RFID transition zone 26 is designed to transition from a smaller cross section of the elongated body 20 to a larger cross section of the RFID section 16, while allowing the resting side of the elongated body 22 and the RFID section 16 to be straight for easy handling.

[0117] The RFID tag is manually inserted into the RFID chamber 28 through the opening 32 under ambient conditions. The RFID tag is inserted at an angle that allows a portion of the RFID tag to enter a portion of the RFID chamber 28 that is beyond the RFID opening 32 and adjacent to the RFID termination area 30. The locking mechanism 34 includes a circular tab 39 that bends downward when the RFID tag 38 is loaded into the RFID chamber 28 and is located above the RFID tag 38 after the RFID tag 38 is fully inserted into the RFID chamber 28. The locking mechanism 34 holds the RFID tag 38 within the RFID chamber 28. The RFID tag is securely held in the RFID chamber 28 and cannot be removed from the RFID chamber 28 by shaking the elongated rod 12. The elongated rod 12 is immersed in liquid nitrogen. When the elongated rod 12 is immersed in liquid nitrogen for approximately 2 minutes and then returned to ambient conditions, the RFID section 16 shows no visible signs of cracking. The RFID segment 16 was viewed at 10X magnification, and again no signs of cracking were observed.

[0118] Comparative Example 2

[0119] Design and injection mold the elongated rod 12. Figure 4a , Figure 4b , Figure 4c 4d, the elongated rod includes an RFID segment 16, a circumferential groove 18a, an elongated body 20, a truncated cone-shaped boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the truncated cone-shaped boss 22. The circumferential groove 18a is located between the end of the elongated body 20 away from the sample collection tip 24 and the RFID segment 16. The RFID segment 16 includes an RFID transition zone 26, an RFID chamber 28, and an RFID terminal zone 30. The RFID terminal zone 30 is positioned farther away from the elongated body 20 along the longitudinal axis than the RFID transition zone 26, and the RFID chamber 28 is located between the RFID terminal zone 30 and the RFID transition zone 26. The size of the RFID chamber 28 is designed to hold an RFID tag (not shown). As such, the RFID chamber 28 is cylindrical in shape, and the opening 32 is circular. In Figure 4a One of the two longitudinal slits 40a is shown in FIG. Figure 4b and Figure 4c Two longitudinal slits 40a, 40b are shown in FIG. 2. In this comparative example, the longitudinal slits are of equal size and each slit begins at the RFID opening 32 and removes a piece of the wall of the RFID chamber 28 that is approximately 90% of the length. Figure 4b yes Figure 4a A cross-sectional view of the elongated rod 12 is shown, and Figure 4c yes Figure 4a A detailed cross-sectional view of the RFID section 16 of the elongated shaft 12 is shown. The locking mechanism includes an "elbow" or ledge 42a, 42b on at least a portion of the wall of the RFID chamber 28 nearest the RFID opening 32.

[0120] Under ambient conditions, an RFID tag is inserted into the RFID chamber 28 through the opening 32. The RFID tag cannot be removed from the RFID chamber 28 by shaking the elongated rod 12. However, because the RFID tag can be removed from the RFID chamber 28 by simply moving a block of the wall of the RFID chamber 28 in an outward direction, the RFID tag is not securely held in the RFID chamber 28. The elongated rod 12 with the RFID tag in the RFID chamber 28 is immersed in liquid nitrogen. The RFID segment 16 is inserted into the liquid nitrogen for approximately 2 minutes and then returned to ambient conditions, and the RFID segment 16 shows no visible signs of rupture. The RFID segment 16 is observed at 10X magnification, and still no signs of rupture are observed. This design only addresses one of the two necessary criteria, namely that the RFID segment 16 does not rupture, but the RFID tag is not securely held in place during processing in the cryopreservation device.

[0121] Example 3

[0122] Design and injection mold the elongated rod 12. Figure 5a , Figure 5b and Figure 5c , the elongated rod includes an RFID segment 16, a circumferential groove 18a, an elongated body 20, a truncated cone-shaped boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the truncated cone-shaped boss 22. The circumferential groove 18a is located between the end of the elongated body 20 away from the sample collection tip 24 and the RFID segment 16. The RFID segment 16 includes an RFID transition zone 26, an RFID chamber 28, and an RFID terminal zone 30. The RFID terminal zone 30 is positioned farther away from the elongated body 20 along the longitudinal axis than the RFID transition zone 26, and the RFID chamber 28 is located between the RFID terminal zone 30 and the RFID transition zone 26. The size of the RFID chamber 28 is designed to hold an RFID tag (not shown). As such, the RFID chamber 28 is cylindrical in shape, and the opening 32 is circular. In Figure 4a One of the two longitudinal slits 40a is shown in FIG. Figure 4b , Figure 4cTwo longitudinal slits 40a, 40b are shown in FIG. In this example, the longitudinal slits are of different sizes, and the longitudinal slit 40a removes a piece of about 50% of the length of the wall of the RFID chamber 28, while the longitudinal slit 40b removes a piece of about 90% of the length of the wall of the RFID chamber 28. Figure 4b yes Figure 4a A cross-sectional view of the elongated rod 12 is shown, and Figure 4c yes Figure 4a A detailed cross-sectional view of the RFID section 16 of the elongated shaft 12 is shown. The locking mechanism includes a "knock" or land 42a, 42b on at least a portion of the wall of the RFID chamber 28 nearest the RFID opening 32.

[0123] Under ambient conditions, an RFID tag was inserted into the RFID chamber 28 through the opening 32. The RFID tag was firmly held in the RFID chamber 28, and the chip could not be removed from the RFID chamber 28 by shaking the elongated rod 12. Unlike in Comparative Example 2, the RFID tag could not be removed from the RFID chamber 28 by moving the wall of the RFID chamber 28 outward. The elongated rod 12 was immersed in liquid nitrogen. The RFID segment 16 was inserted into the liquid nitrogen for about 2 minutes and then returned to ambient conditions, and the RFID segment 16 showed no visible signs of cracking. The RFID segment 16 was observed at 10X magnification, and still no signs of cracking were observed.

[0124] Figure 6a Another embodiment of a closed system cryopreservation device 10 is illustrated, which has an elongated rod 12 and a cap 14 (not shown) with an end-loaded RFID segment 16. The elongated rod 12 includes the RFID segment 16, an elongated body 20, a truncated cone-shaped boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the truncated cone-shaped boss 22. The RFID segment 16 includes an RFID transition region 26, an RFID compartment 28, and an RFID termination region 30. An RFID plug 44 is removably attached to the RFID segment 16. The RFID termination region 30 is located farther away from the elongated body 20 along the longitudinal axis than the RFID transition region 26, and the RFID chamber 28 is located between the RFID termination region 30 and the RFID transition region 26.

[0125] Figure 6b and Figure 6c yes Figure 6a Detailed cross-sectional view of the RFID segment 16 of the illustrated embodiment. Figure 6b is a cross-sectional view illustrating an empty RFID chamber 28 in which the RFID tag 38 and the RFID plug 44 are separated from the RFID section 16 . Figure 6c4 is a cross-sectional view illustrating an RFID tag 38 positioned within the RFID chamber 28 and secured by an RFID plug 44. In this embodiment, the RFID chamber 28 is slightly oversized relative to the RFID tag 38 to allow for heat shrinkage when the cryopreservation device is submerged in liquid nitrogen. The RFID plug 44 is used to Figure 2a to Figure 2d and Figure 3a to Figure 3c By blocking the RFID opening 32, the RFID plug 44 retains the RFID tag 38 in the RFID chamber 28 throughout the handling and storage of the cryopreservation device.

[0126] Figure 7 Another embodiment of a cryogenic storage device 10 is shown having an elongated stem 12 and a cap 14 (not shown) with an alternative end-loaded RFID segment 16. The elongated stem 12 includes the RFID segment 16, a circumferential groove 18a, an elongated body 20, a truncated cone-shaped boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the boss 22. The circumferential groove 18a is located between the end of the elongated body 20 distal to the sample collection tip 24 and the RFID segment 16. The RFID segment 16 includes an RFID transition region 26, an RFID chamber 28, and an RFID terminal region 30 that accommodates an RFID plug 44. The RFID terminal region 30 is located farther away from the elongated body 20 along the longitudinal axis than the RFID transition region 26, and the RFID chamber 28 is located between the RFID terminal region 30 and the RFID transition region 26. Figure 7 1. In the embodiment of the present invention, the entirety of the RFID segment 16 is tapered or gradually becomes thinner, giving the RFID segment 16 a larger cross-section the further it is removed from the elongated body 20.

[0127] Figure 8a and Figure 8bAnother embodiment of a cryopreservation device 10 having an elongated stem 12 and a cap 14 (not shown) is shown. The elongated stem 12 includes a removable attachment member 50, an elongated body 20, a truncated cone-shaped boss 22 extending from a first end of the elongated body, and a sample collection tip 24 extending from a narrow end of the truncated cone-shaped boss 22. The removable attachment member 50 includes an RFID section 16, a groove 18a, and an RFID connection element 52. The elongated body 20 includes a compatible body connection element 54. The RFID connection element 52 includes a transition region 56 and an RFID locking mechanism 58. When the removable attachment member 50 is attached to the elongated body 20, the transition region 56 is away from the elongated body 20, and the RFID locking mechanism 58 is located between the transition region 56 and the elongated body 20. The RFID locking mechanism 58 includes a hollow chamber 60, wherein the grooves 62a and 62b are toward the end of the hollow chamber 60 that is closest to the transition region 56. The compatible body connection element 54 is located on the elongated body 20 away from the sample collection tip 24 and includes a solid object 64 and extensions 66a and 66b. The extensions 66a and 66b are located toward the end of the compatible body connection element 54 away from the elongated body 20. When the removable attachment member 50 is attached to the elongated body 20, the solid object 64 is sized to fit in the chamber 60, and the extensions 66a and 66b are sized to fit into the grooves 62a and 62b.

[0128] Figure 8c The diagram shows the Figure 8b 1. An alternative locking mechanism of the RFID connection element 52 and the compatible body connection element 54 of the removable attachment component 50 is shown. The removably attachable RFID connection element 50 includes the RFID section 16 and the RFID connection element 70. The RFID connection element 70 includes a solid object 72 with extensions 74a and 74b. When the removable attachment component 50 is attached to the elongated body 20, the RFID transition zone 26 is away from the elongated body 20, and the solid object 72 is located between the RFID transition zone 26 and the elongated body 20. The extensions 74a and 74b are located toward the end of the solid object 72 away from the RFID transition zone 26. The compatible body locking mechanism 76 includes a hollow chamber 78 with grooves 80a and 80b. When the removable attachment component 50 is attached to the elongated body 20, the compatible body locking mechanism 76 is sized to receive the solid object 72, and the grooves 80a and 80b are sized to receive the extensions 74a and 74b.

[0129] Figure 9aAnother embodiment of a cryogenic storage device 10 is illustrated, having an elongated stem 12 and a cap 14 (not shown), with another embodiment of a top-loaded RFID segment 16. The elongated stem 12 includes a recess 18a, an RFID segment 16, an elongated body 20, a frustoconical boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the frustoconical boss 22. The RFID segment 16 includes an RFID transition region 26, an RFID chamber 28, and an RFID termination region 30.

[0130] Figure 9b , Fig.9c and Figure 9d yes Figure 9a Detailed view of the RFID segment 16 of the illustrated embodiment. Figure 9b is a cross-sectional view illustrating an RFID tag 38 located in the RFID chamber 28. The edge of the RFID tag 38 is not visible because the locking mechanism 34 extends along the length of the RFID chamber 28 and once the RFID tag 38 is loaded into the RFID chamber 28, the locking mechanism seats on the top of the side of the RFID tag 38. Fig.9c is a top view illustrating the locking mechanism 34 that defines the opening 32 at the top of the RFID chamber 28. When the RFID tag 38 is fully inserted into the RFID chamber 28, the locking mechanism portions 82a and 82b extend over the RFID tag 38. The locking mechanism portions 82a and 82b are capable of retaining the RFID tag 38 within the RFID chamber 28.

[0131] Although the present invention has been illustrated and described herein with reference to preferred embodiments and specific examples of the present invention, it is apparent to one of ordinary skill in the art that other embodiments and examples may perform similar functions and / or achieve similar results. All such equivalent embodiments and examples are within the spirit and scope of the present invention and are intended to be covered by the appended claims.

Claims

1. A cryogenic storage device, comprising: a) an elongated rod comprising an RFID section, an elongated body and a sample collection tip; and b) a cap comprising a hollow chamber, wherein the hollow chamber has a length sufficient to accommodate the sample collection tip, wherein, when the cap is removably attached to the elongated rod, the cap is capable of enclosing the sample collection tip within the hollow chamber, wherein the RFID section is far away from the sample collection tip, and Wherein, the RFID section includes: a) an RFID chamber capable of holding an RFID tag; b) an RFID opening through which the RFID tag can be placed into the RFID chamber; and c) a locking mechanism abutting a perimeter of the RFID opening, and wherein the RFID opening is located at the end of the elongated rod, wherein the RFID chamber comprises a substantially cylindrical chamber made of plastic with a prismatic shape as an outline, wherein the RFID tag is substantially cylindrical, and wherein the prismatic shape has a first longitudinal slit and a second longitudinal slit, each slit starting from the RFID opening.

2. The cryopreservation device of claim 1 , further comprising a truncated cone-shaped boss extending from the first end of the elongated body, wherein the sample collection tip extends from the truncated cone-shaped boss, and wherein when the cap is removably attached to the elongated rod, the cap is capable of enclosing the sample collection tip and the truncated cone-shaped boss within the hollow chamber.

3. The cryopreservation device according to claim 1, wherein: The elongated rod is made from a single piece of plastic.

4. The cryopreservation device according to claim 1, wherein: When the RFID tag was placed in the RFID chamber and the cryogenic storage device was placed in liquid nitrogen for 5 minutes, the RFID tag remained in the RFID chamber and the cryogenic storage device showed no visible cracks.

5. The cryopreservation device according to claim 1, wherein: The RFID tags have a length ranging from 4 mm to 12 mm long and a diameter ranging from 0.5 mm to 4 mm.

6. The cryopreservation device according to claim 1, wherein: The first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber, and wherein the first longitudinal slit is longer than the second longitudinal slit.

7. The cryopreservation device according to claim 1, wherein: The first longitudinal slit traverses the RFID chamber from the second longitudinal slit.

8. The cryopreservation device according to claim 1, wherein: The locking mechanism includes a platform at least partially surrounding the perimeter of the RFID opening.

9. The cryopreservation device according to claim 1, wherein: The RFID segment has a dimension of no greater than about 3 mm in each of an x-direction and a y-direction perpendicular to the longitudinal direction.

10. The cryopreservation device according to claim 1, wherein: The RFID segment has a dimension of no greater than about 2.4 mm in each of an x-direction and a y-direction perpendicular to the longitudinal direction.

11. A cryogenic storage device, comprising: a) an elongated rod comprising an RFID section, an elongated body, a truncated cone-shaped boss extending from a first end of the elongated body, and a sample collection tip extending from the truncated cone-shaped boss; and b) a cap comprising a hollow chamber, wherein the hollow chamber has a length sufficient to accommodate the sample collection tip and the truncated cone-shaped boss, wherein, when the cap is removably attached to the elongated rod, the cap is capable of enclosing the sample collection tip within the hollow chamber, wherein the RFID section is far away from the sample collection tip, wherein the RFID segment is capable of holding an RFID tag, and Wherein, the RFID section includes: a) an RFID chamber, the RFID chamber being capable of holding the RFID tag; b) an RFID opening through which the RFID tag can be placed into the RFID chamber; and c) a locking mechanism abutting a periphery of the RFID opening, wherein the RFID opening is located at the end of the elongated rod, wherein the RFID chamber comprises a substantially cylindrical chamber made of plastic with a prismatic shape as an outline, wherein the RFID tag is substantially cylindrical, and wherein the prismatic shape has a first longitudinal slit and a second longitudinal slit, each slit starting from the RFID opening.

12. The cryopreservation device according to claim 11, wherein The elongated rod is made of one piece of plastic and wherein the RFID tag has a length ranging from 6 mm to 12 mm long and a diameter ranging from 0.5 mm to 4 mm.

13. The cryopreservation device according to claim 11, wherein The locking mechanism includes a platform at least partially surrounding the RFID opening.

14. The cryopreservation device according to claim 11, wherein The first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber, and Wherein, the first longitudinal slit is longer than the second longitudinal slit, and wherein the first longitudinal slit traverses the RFID chamber from the second longitudinal slit.

15. The cryopreservation device according to claim 14, wherein: The RFID segment has a dimension of no greater than about 2.4 mm in each of an x-direction and a y-direction perpendicular to the longitudinal direction.

16. The cryopreservation device according to claim 14, wherein: The elongated rod is made from a single piece of plastic.

17. The cryopreservation device according to claim 11, wherein: When the RFID tag was placed in the RFID chamber and the cryogenic storage device was placed in liquid nitrogen for 5 minutes, the RFID tag remained in the RFID chamber and the cryogenic storage device showed no visible cracks.

18. A cryogenic storage device, comprising: a) an elongated rod comprising an RFID section, an elongated body, a truncated cone-shaped boss extending from a first end of the elongated body, and a sample collection tip extending from the truncated cone-shaped boss; and b) a cap comprising a hollow chamber, wherein the hollow chamber has a length sufficient to accommodate the sample collection tip and the truncated cone-shaped boss, wherein, when the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip and the truncated cone-shaped boss within the hollow chamber, Wherein, the RFID section is far away from the sample collection tip, and the RFID section is capable of holding an RFID tag, wherein the RFID section comprises: a) an RFID chamber, the RFID chamber being capable of holding the RFID tag; b) an RFID opening through which the RFID tag can be placed into the RFID chamber; and c) a locking mechanism abutting a periphery of the RFID opening, Wherein, the elongated rod is made of a plastic integral piece, wherein the RFID opening is located at the end of the elongated rod, wherein the RFID chamber comprises a cylindrical chamber made of plastic with a prismatic shape as an outline, wherein the RFID tag is cylindrical, and wherein the prismatic shape has a first longitudinal slit and a second longitudinal slit, each slit starting from the RFID opening, wherein the first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber, and wherein the first longitudinal slit is longer than the second longitudinal slit, wherein the first longitudinal slit extends across the RFID chamber from the second longitudinal slit, and Wherein, the locking mechanism includes a platform at least partially surrounding the periphery of the RFID opening.

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