Tissue culture and rapid propagation method of turmeric

Abstract

The invention relates to the technical field of plant tissue culture, in particular to a rapid propagation method for tissue culture of turmeric. The method comprises the following steps: by taking turmeric tender shoots as explants, carrying out induction culture, multiplication culture and rooting culture; a proliferation culture medium for proliferation culture further comprises 6-BA, IBA, NAA, mashed potatoes and cane sugar on the basis of an MS culture medium; the dosage of the mashed potatoes is 10-40g / L. According to the method, tender shoots of turmeric are used as explants, turmeric seedlings are produced through an in-vitro rapid propagation method, mashed potatoes are added into a proliferation culture medium, the proliferation efficiency of cluster buds is effectively improved, then large-scale rapid propagation of turmeric can be achieved, the problem of the proliferation rate of turmeric is solved, and the yield of turmeric seedlings is increased. The method is of great significance to large-scale planting, popularization and utilization of turmeric.

Description

Technical Field

[0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method for turmeric. Background Art

[0002] Turmeric (Latin name: Curcuma longa L.), also known as: turmeric, Baodingxiang, Haoming, yellow ginger, etc., belongs to the Curcumales, Zingiberaceae, and Curcuma genus perennial herb, with a plant height of 1~1.5m, well-developed rhizomes, thick roots, and tuberous roots at the end; oblong or elliptical leaves, short and gradually pointed at the top; ovate or oblong bracts, light green, blunt at the top, and light yellow corolla; flowering period is August. Turmeric has the effects of promoting qi and removing blood stasis, promoting menstruation and relieving pain. It is mainly used to treat chest and abdominal distension and pain, shoulder and arm pain, unbearable heart pain, postpartum blood pain, initial onset of sores and ringworms, irregular menstruation, amenorrhea, and traumatic injuries. It can also be used to extract yellow edible dyes; the curcumin contained can be used as an analytical chemical reagent.

[0003] At present, turmeric is usually propagated by rhizomes. The rhizomes are buried in the ground in a certain season for propagation, and the new growth plants sprouting from the rhizomes are generally used as seedlings. However, this propagation method is not only limited by the seasonal climate, but also has a very low proliferation rate, which seriously hinders the large-scale rapid propagation of turmeric and cannot guarantee the stability of turmeric's horticultural traits. Summary of the invention

[0004] In order to solve the problems existing in the prior art, the present invention provides a method for rapid propagation of turmeric through tissue culture.

[0005] In the process of plant tissue culture, the culture medium usually used is configured according to the nutrients required for plant growth. MS medium (Murashige and Skoog medium) is often used, which contains macro- and trace elements, organic carbon sources, amino acids, vitamins, etc. required for plant growth. Based on MS, technicians will add plant hormones (cytokinins, gibberellins, etc.) according to the needs of the plants, or further supplement carbon sources.

[0006] Potatoes are often not a conventional culture medium component for tissue culture. This is because potatoes have complex nutrients and are often not considered to provide precise nutrients (such as inorganic salts, vitamins, and amino acids), and most of the organic substances contained in potatoes are not in a form that can be directly used by plants. However, the present invention accidentally discovered in experimental research that in the tissue culture process of turmeric sprouts, adding mashed potatoes to the proliferation culture medium can significantly improve the efficiency of clustered sprout proliferation. This may be because certain nutrients in potatoes play a role in promoting clustered sprout proliferation. However, the amount of mashed potatoes should not be too much, as it may affect the structural state of the culture medium and thus have an adverse effect on the physiological process of the plant.

[0007] In a first aspect, the present invention provides a method for tissue culture of turmeric, comprising: using turmeric sprouts as explants, and subjecting them to induction culture, proliferation culture and rooting culture; The proliferation culture medium used for the proliferation culture is based on the MS culture medium and further includes: 6-BA, IBA, NAA, mashed potatoes and sucrose; the amount of the mashed potatoes is 10-40g / L.

[0008] Furthermore, in parts by weight, the proliferation medium is based on the MS medium and further comprises: 1-3 mg / L 6-BA, 0.2-1 mg / L IBA, 0.05-0.2 mg / L NAA, 10-40 g / L mashed potato, 20-50 g / L sucrose and 5-15 g / L carrageenan.

[0009] Furthermore, the conditions for the proliferation culture include: a culture temperature of 25-28° C., a light exposure time of 8-12 hours / day, and a light intensity of 2000-3000 lx.

[0010] Furthermore, the induction culture medium used for the induction culture is based on the MS culture medium and also includes: 6-BA 1~3 mg / L + IBA 0.2~1 mg / L + sucrose 20~50 g / L + carrageenan 5~15 g / L.

[0011] Furthermore, the induction culture conditions include: a culture temperature of 25-28°C.

[0012] Furthermore, the rooting medium used for the rooting culture is based on the 1 / 2MS medium and also includes: NAA 0.05-0.2 mg / L, sucrose 15-40 g / L, carrageenan 5-15 g / L and activated carbon 0.2-2 g / L.

[0013] Furthermore, the rooting culture conditions include: a culture temperature of 26-30° C., a light exposure time of 9-13 hours / day, a light intensity of 2000-3000 lx, and rooting culture for 3-5 times.

[0014] Furthermore, the turmeric sprouts are sprouts from mature turmeric tubers, and the process includes cleaning and disinfection before induction.

[0015] In a second aspect, the present invention provides a proliferation culture medium, which, based on the MS culture medium, further comprises: 1-3 mg / L 6-BA, 0.2-1 mg / L IBA, 0.05-0.2 mg / L NAA, 10-40 g / L mashed potatoes, 20-50 g / L sucrose and 5-15 g / L carrageenan.

[0016] In a third aspect, the present invention provides application of the method in improving the rapid propagation ability of turmeric tissue culture.

[0017] Furthermore, the application includes: improving the explant induction rate, or improving the cluster bud proliferation coefficient.

[0018] The present invention has the following beneficial effects: The invention uses turmeric sprouts as explants, produces turmeric seedlings through an in vitro rapid propagation method, adds a certain amount of mashed potatoes into a proliferation culture medium, effectively improves the proliferation coefficient of cluster sprouts, and further improves the rapid propagation effect of turmeric tissues.

[0019] The present invention further provides specific induction culture, proliferation culture and rooting culture processes, which enable the induction rate of explants to reach more than 86%, the proliferation coefficient of clustered buds to reach more than 4.8, and the rooting rate of tissue culture seedlings to reach more than 100%.

[0020] The invention realizes large-scale rapid propagation of turmeric, can quickly and effectively produce a large number of high-quality turmeric seedlings, is suitable for factory production of seedlings, breaks through the traditional method of propagation by rhizomes, solves the problem of low proliferation rate of turmeric, and is of great significance to the large-scale planting and promotion and utilization of turmeric. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0022] Figure 1 This is a diagram of the turmeric sprout explant provided in Example 1 of the present invention.

[0023] Figure 2 This is a diagram of turmeric clustered sprouts provided in Example 1 of the present invention.

[0024] Figure 3 This is a diagram of the turmeric bud clusters in the proliferation culture provided in Example 1 of the present invention.

[0025] Figure 4 This is a diagram of the rooted turmeric seedlings in root culture provided in Example 1 of the present invention. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The experimental methods involved in the following examples, unless otherwise mentioned, are all conventional methods in the art, for example, reference may be made to experimental manuals in the art, or the conditions recommended in the manufacturer's instructions.

[0028] Unless otherwise specified, the experimental materials and reagents involved in the following examples can be obtained from commercial sources.

[0029] The sugar used in the embodiment of the present invention is sucrose, which can be purchased commercially.

[0030] The activated carbon used in the embodiment of the present invention is 200 mesh activated carbon powder.

[0031] The mashed potatoes used in the embodiment of the present invention are fresh potatoes that are cooked and squeezed into mash.

[0032] The MS culture medium and 1 / 2MS culture medium used in the examples of the present invention were commercially available (Hangzhou Best).

[0033] Example 1 The present invention provides a method for rapid propagation of turmeric tissue culture using tender shoots as explants, which is mainly different from Example 1 in that mashed potatoes are further added to the proliferation culture medium, and comprises the following steps: 1. Selection and disinfection of explants: Select turmeric sprouts sprouted from mature tubers as explants, wash the sand on the sprouts with tap water, then soak them in a solution diluted with laundry detergent for 30 minutes, then rinse them with running water for 30 minutes, then disinfect them with 75% alcohol by volume for 30 seconds on a clean bench, then disinfect them with 0.1% mercuric chloride by mass for 7 minutes, and finally wash them with sterile water for 3-4 times. Figure 1 shown.

[0034] 2. Induction of clustered buds: Cut the explants after sterilization into small pieces and inoculate them into induction medium for 20 days for clustered bud induction culture. The culture temperature is 25-28℃ (it is difficult to keep the temperature constant during the culture process, so the temperature is actually controlled within a certain range). The induction medium is: MS+2.0mg / L 6-BA+0.5mg / L IBA+30g / L sugar+8g / L carrageenan. Figure 2 shown.

[0035] 3. Proliferation culture of clustered buds: The induced turmeric clustered buds were inoculated into a proliferation culture medium for 30 days at a culture temperature of 25-28°C, a light duration of 10 h / day, and a light intensity of 2500 lx; ​​the proliferation culture medium was: MS + 2.0 mg / L 6-BA + 0.5 mg / L IBA + 0.1 mg / L NAA + 30 g / L mashed potatoes + 30 g / L sugar + 9 g / L carrageenan. Figure 3 shown.

[0036] 4. Rooting culture: The turmeric proliferating buds obtained by 5 times of proliferation culture were inoculated into the rooting medium for rooting culture for 50 days, with the culture temperature of 26-30℃, the light time of 11h / day, and the light intensity of 2500lx to obtain rooting seedlings; the rooting medium was 1 / 2MS+0.1mg / L NAA+25g / L sugar+9g / L carrageenan+0.5g / L activated carbon. Figure 4 shown.

[0037] Comparative Example 1 The present invention provides a method for rapid propagation of turmeric tissue culture using turmeric sprouts as explants, which comprises the following steps: 1. Selection and disinfection of explants: Select turmeric sprouts sprouted from mature tubers as explants, wash the mud and sand on the sprouts with tap water, then soak them in a solution diluted with laundry detergent for 30 minutes, then rinse them with running water for 30 minutes, then disinfect them with 75% alcohol by volume for 30 seconds on an ultra-clean workbench, then disinfect them with 0.1% mercuric chloride by mass for 7 minutes, and finally wash them with sterile water 3-4 times.

[0038] 2. Induction of clustered buds: The detoxified explants were cut into small pieces and inoculated into induction medium for clustered bud induction culture for 20 days at a temperature of 25-28°C; the induction medium was: MS + 2.0 mg / L 6-BA + 0.5 mg / L IBA + 30 g / L sugar + 8 g / L carrageenan.

[0039] 3. Proliferation culture of clustered shoots: The induced turmeric clustered shoots were inoculated into a proliferation culture medium for proliferation culture for 30 days. The culture temperature was 25-28°C, the light duration was 10 h / day, and the light intensity was 2500 lx. The proliferation culture medium was: MS + 2.0 mg / L 6-BA + 0.5 mg / L IBA + 0.1 mg / L NAA + 30 g / L sugar + 9 g / L carrageenan.

[0040] 4. Rooting culture: The turmeric proliferating buds obtained after 5 times of proliferation culture were inoculated into rooting medium for rooting culture for 50 days, with a culture temperature of 26-30°C, a lighting time of 11h / day, and a light intensity of 2500lx to obtain rooted seedlings; the rooting medium was 1 / 2MS+0.1mg / L NAA+25g / L sugar+9g / L carrageenan+0.5g / L activated carbon.

[0041] The amount of sucrose used in this comparative example is obtained through optimization. In actual culture, if the sucrose content is further added on the basis of this comparative example, the explant induction rate and the cluster bud proliferation coefficient cannot be further improved.

[0042] Comparative Example 2 The present invention provides a method for rapid propagation of turmeric tissue culture using tubers as explants, which comprises the following steps: 1. Selection and disinfection of explants: Select turmeric sprouts sprouted from mature tubers as explants, wash the mud and sand on the sprouts with tap water, then soak them in a solution diluted with laundry detergent for 30 minutes, then rinse them with running water for 30 minutes, and then disinfect them with 75% alcohol by volume for 30 seconds on an ultra-clean workbench, then disinfect them with 0.1% mercuric chloride solution by mass for 7 minutes, and finally wash them with sterile water 3-4 times.

[0043] 2. Induction of clustered buds: The detoxified explants were cut into small pieces and inoculated into induction medium for clustered bud induction culture for 20 days at a temperature of 25-28°C; the induction medium was: MS + 3.0 mg / L 6-BA + 0.5 mg / L IBA + 30 g / L sugar + 8 g / L carrageenan.

[0044] 3. Proliferation culture of clustered shoots: The induced turmeric clustered shoots were inoculated into a proliferation culture medium for proliferation culture for 30 days. The culture temperature was 25-28°C, the light duration was 10 h / day, and the light intensity was 3000 lx. The proliferation culture medium was: MS + 2.0 mg / L 6-BA + 1.0 mg / L IBA + 0.1 mg / L NAA + 30 g / L sugar + 9 g / L carrageenan.

[0045] 4. Rooting culture: The turmeric proliferating buds obtained by proliferation culture 5 times were inoculated into rooting medium for rooting culture for 50 days, with a culture temperature of 26-30°C, a lighting time of 11h / day, and a light intensity of 3000lx to obtain rooted seedlings; the rooting medium was: MS + 0.1mg / L NAA + 30g / L sugar + 9g / L carrageenan + 0.5g / L activated carbon.

[0046] Comparative Example 3 The present invention provides a turmeric tissue culture rapid propagation method using tender shoots as explants, which is mainly different from Comparative Example 1 in that the concentrations of 6-BA in the induction medium and the proliferation medium are different, and the method comprises the following steps: 1. Selection and disinfection of explants: Select turmeric sprouts sprouted from mature tubers as explants, wash the mud and sand on the sprouts with tap water, then soak them in a solution diluted with laundry detergent for 30 minutes, then rinse them with running water for 30 minutes, then disinfect them with 75% alcohol by volume for 30 seconds on an ultra-clean workbench, then disinfect them with 0.1% mercuric chloride by mass for 7 minutes, and finally wash them with sterile water 3-4 times.

[0047] 2. Induction of clustered buds: The detoxified explants were cut into small pieces and inoculated into induction medium for clustered bud induction culture for 20 days at a temperature of 25-28°C; the induction medium was: MS + 1.0 mg / L 6-BA + 0.5 mg / L IBA + 30 g / L sugar + 8 g / L carrageenan.

[0048] 3. Proliferation culture of clustered shoots: The induced turmeric clustered shoots were inoculated into a proliferation culture medium for proliferation culture for 30 days. The culture temperature was 25-28°C, the light duration was 10 h / day, and the light intensity was 2500 lx. The proliferation culture medium was: MS + 1.0 mg / L 6-BA + 0.5 mg / L IBA + 0.1 mg / L NAA + 30 g / L sugar + 9 g / L carrageenan.

[0049] 4. Rooting culture: The turmeric proliferating buds obtained by proliferation culture 5 times were inoculated into rooting medium for rooting culture for 50 days, with a culture temperature of 26-30°C, a lighting time of 11h / day, and a light intensity of 2500lx to obtain rooted seedlings; the rooting medium was: 1 / 2MS + 0.1mg / L NAA + 25g / L sugar + 9g / L carrageenan + 0.5g / L activated carbon.

[0050] Comparative Example 4 The present invention provides a turmeric tissue culture rapid propagation method using tender shoots as explants, which is mainly different from Comparative Example 1 in that the concentrations of 6-BA in the induction medium and the proliferation medium are different, and the method comprises the following steps: 1. Selection and disinfection of explants: Select turmeric sprouts sprouted from mature tubers as explants, wash the mud and sand on the sprouts with tap water, then soak them in a solution diluted with laundry detergent for 30 minutes, then rinse them with running water for 30 minutes, then disinfect them with 75% alcohol by volume for 30 seconds on an ultra-clean workbench, then disinfect them with 0.1% mercuric chloride by mass for 7 minutes, and finally wash them with sterile water 3-4 times.

[0051] 2. Induction of clustered buds: The explants after sterilization were cut into small pieces and inoculated into induction medium for clustered bud induction culture for 20 days at a temperature of 25-28°C; the induction medium was: MS + 3.5 mg / L 6-BA + 0.5 mg / L IBA + 30 g / L sugar + 8 g / L carrageenan.

[0052] 3. Proliferation culture of clustered shoots: The induced turmeric clustered shoots were inoculated into a proliferation culture medium for proliferation culture for 30 days. The culture temperature was 25-28°C, the light duration was 10 h / day, and the light intensity was 2500 lx. The proliferation culture medium was: MS + 3.5 mg / L 6-BA + 0.5 mg / L IBA + 0.1 mg / L NAA + 30 g / L sugar + 9 g / L carrageenan.

[0053] 4. Rooting culture: The turmeric proliferating buds obtained by proliferation culture 5 times were inoculated into rooting medium for rooting culture for 50 days, with a culture temperature of 26-30°C, a lighting time of 11h / day, and a light intensity of 2500lx to obtain rooted seedlings; the rooting medium was: 1 / 2MS + 0.1mg / L NAA + 25g / L sugar + 9g / L carrageenan + 0.5g / L activated carbon.

[0054] Comparative Example 5 The present invention provides a turmeric tissue culture rapid propagation method using tender shoots as explants, which is mainly different from Example 1 in that the amount of mashed potatoes added to the proliferation medium is different, and the method comprises the following steps: 1. Selection and disinfection of explants: Select turmeric sprouts sprouted from mature tubers as explants, wash the mud and sand on the sprouts with tap water, then soak them in a solution diluted with laundry detergent for 30 minutes, then rinse them with running water for 30 minutes, then disinfect them with 75% alcohol by volume for 30 seconds on an ultra-clean workbench, then disinfect them with 0.1% mercuric chloride by mass for 7 minutes, and finally wash them with sterile water 3-4 times.

[0055] 2. Induction of clustered buds: The detoxified explants were cut into small pieces and inoculated into induction medium for clustered bud induction culture for 20 days at a temperature of 25-28°C; the induction medium was: MS + 2.0 mg / L 6-BA + 0.5 mg / L IBA + 30 g / L sugar + 8 g / L carrageenan.

[0056] 3. Proliferation culture of clustered shoots: The induced turmeric clustered shoots were inoculated into a proliferation culture medium for proliferation culture for 30 days. The culture temperature was 25-28°C, the light duration was 10 h / day, and the light intensity was 2500 lx. The proliferation culture medium was: MS + 2.0 mg / L 6-BA + 0.5 mg / L IBA + 0.1 mg / L NAA + 60 g / L mashed potatoes + 30 g / L sugar + 9 g / L carrageenan.

[0057] 4. Rooting culture: The turmeric proliferating buds obtained after 5 times of proliferation culture were inoculated into rooting medium for rooting culture for 50 days, with a culture temperature of 26-30°C, a lighting time of 11h / day, and a light intensity of 2500lx to obtain rooted seedlings; the rooting medium was 1 / 2MS+0.1mg / L NAA+25g / L sugar+9g / L carrageenan+0.5g / L activated carbon.

[0058] Experimental Example 1 The present invention is tested according to each embodiment and comparative example respectively, and embodiments 1-3 and comparative examples 1-3 are test groups 1-6 in turn, and each test process is 100 small pieces of explants, and the number of clustered buds formed is recorded, and the induction rate of explants in each test group is calculated, the number of new bud proliferation is recorded, and the proliferation coefficient of clustered buds and the rooting rate of tissue culture seedlings are calculated. The results are shown in Table 1.

[0059] Explant induction rate = number of explants that formed clustered shoots / total number of inoculated explants*100%; Cluster bud proliferation coefficient = number of proliferating buds / total number of inoculated cluster buds; Rooting rate = number of roots of tissue culture seedlings / total number of turmeric tissue culture seedlings*100%.

[0060] Table 1 Bamboo sapogenin tissue culture

[0061] The present invention uses turmeric sprouts as explants, produces bamboo shoot seedlings through an in vitro rapid propagation method, and optimizes and screens an induction medium for the explants, a proliferation medium for clustered sprouts, and a rooting medium for tissue culture seedlings, so that the induction rate of the explants can reach more than 92%, the proliferation coefficient of the clustered sprouts can reach more than 4.3, the rooting rate of the tissue culture seedlings can reach more than 100%, and the root system has strong growth vitality (the average root system length in Example 1 is 10-15 cm).

[0062] Among them, Comparative Examples 1 and 2 adjusted the proliferation medium and did not use mashed potatoes (other nutrients were sufficient, such as carbon source), and the cluster bud proliferation coefficient was significantly reduced. Comparative Examples 3 and 4 adjusted the 6-BA concentration in the explant induction medium and the cluster bud proliferation medium on the basis of Comparative Example 1, and their explant induction rate and cluster bud proliferation coefficient were significantly reduced, indicating that a certain concentration of 6-BA combined with IBA and NAA can effectively improve the induction and proliferation effects of the explant induction medium and the cluster bud proliferation medium.

[0063] In Example 1 of the present invention, a certain amount of mashed potatoes is added to the proliferation medium, which effectively improves the proliferation coefficient of the clustered buds. Compared with Example 1, in Comparative Example 5, more mashed potatoes are added, but the increase in the proliferation coefficient of the clustered buds is reduced.

[0064] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for tissue culture of turmeric, characterized in that: include: Using turmeric sprouts as explants, the culture was induced, multiplied and rooted. The proliferation culture medium used for the proliferation culture is based on the MS medium and further includes: 6-BA, IBA, NAA, mashed potatoes and sucrose; The amount of the mashed potatoes is 10-40 g / L.

2. The tissue culture method according to claim 1, characterized in that In parts by weight, the proliferation medium is based on the MS medium and further comprises: 1-3 mg / L 6-BA, 0.2-1 mg / L IBA, 0.05-0.2 mg / L NAA, 10-40 g / L mashed potato, 20-50 g / L sucrose and 5-15 g / L carrageenan.

3. The tissue culture method according to claim 2, characterized in that The conditions for the proliferation culture include: a culture temperature of 25-28° C., a light exposure time of 8-12 hours / day, and a light intensity of 2000-3000 lx.

4. The tissue culture method according to any one of claims 1 to 3, characterized in that: The induction culture medium used for the induction culture is based on the MS culture medium and further includes: 6-BA 1~3mg / L + IBA 0.2~1mg / L + sucrose 20~50g / L + carrageenan 5~15g / L.

5. The tissue culture method according to claim 4, characterized in that The induction culture conditions include: a culture temperature of 25-28°C.

6. The tissue culture method according to any one of claims 1 to 5, characterized in that: The rooting medium used for the rooting culture is based on 1 / 2MS medium and further includes: NAA 0.05-0.2 mg / L, sucrose 15-40 g / L, carrageenan 5-15 g / L and activated carbon 0.2-2 g / L.

7. The tissue culture method according to claim 6, characterized in that The rooting culture conditions include: a culture temperature of 26-30° C., a light exposure time of 9-13 hours per day, a light intensity of 2000-3000 lx, and rooting culture for 3-5 times.

8. The method according to any one of claims 1 to 7, characterized in that: The turmeric sprouts are sprouts on mature turmeric tubers, and are cleaned and disinfected before induction.

9. A proliferation medium, characterized in that The proliferation culture medium further comprises, on the basis of the MS culture medium, 1-3 mg / L 6-BA, 0.2-1 mg / L IBA, 0.05-0.2 mg / L NAA, 10-40 g / L mashed potato, 20-50 g / L sucrose and 5-15 g / L carrageenan.

10. Use of the method according to any one of claims 1 to 8, or the proliferation medium according to claim 9, in improving the rapid propagation ability of turmeric tissue culture.

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