Fish stimulus response behavior tracking and monitoring device and method

Abstract

The present invention discloses a device and method for tracking and monitoring fish stimulus-response behavior, comprising an observation box and an animal behavior trajectory tracking system communicatively connected to the observation box. The observation box comprises a hollow box body on one side, a support frame disposed within the box body, a carrier plate attached to the top of the support frame, a porous plate inserted into the carrier plate, a stimulation mechanism disposed within the support frame for stimulating fish within the porous plate, an infrared camera disposed on the inner wall of the box body above the support frame, the carrier plate movable within the box body via a drive mechanism, and an auxiliary mechanism disposed on the inner wall of the top of the box body, located outside the infrared camera. The present invention provides an auxiliary mechanism, which, after the tracking and monitoring work is completed, can replace manual labor to transfer zebrafish juveniles within the porous plate to a storage container, thereby saving time and significantly reducing labor intensity.

Description

Technical Field

[0001] The present invention relates to the technical field of fish tracking and monitoring, and in particular to a device and method for tracking and monitoring fish stimulus response behavior. Background Art

[0002] Fish stimulus-response behavior tracking and monitoring devices have been widely used in CYN research. Cylindrospermopsin (CYN), a common cyanobacterial toxin, has attracted considerable attention for its effects on aquatic organisms, particularly fish. In current research, researchers typically expose zebrafish larvae to varying concentrations of CYN solution over time. After a certain period of time, these larvae are then placed in a fish stimulus-response behavior tracking and monitoring device for observation and analysis.

[0003] The fish tracking and monitoring device primarily consists of a zebrafish observation chamber and a computer pre-installed with an animal behavior tracking system (including a test hardware control module and a multi-observation zone module). The system collects data from the fish's response to light and sound stimulation within the chamber, which is then transmitted to the animal behavior tracking system. The system then collects and analyzes the juvenile fish's movement trajectories, activity heat maps, and calculates key parameters such as movement distance, idle time, and movement speed. This allows users to understand the effects of CYN on the early nervous system development of zebrafish. However, while the aforementioned tracking and monitoring device can track and monitor fish, it still suffers from the following drawbacks:

[0004] Zebrafish larvae to be tracked and monitored are typically placed into the wells of a multi-well plate and then placed in an observation chamber. After the work is completed, the larvae within the multi-well plate need to be transferred. Currently, this is typically done manually using a pipette to extract the zebrafish from the wells one by one and then transfer them to an external container. This method is not only time-consuming and labor-intensive, but also requires manual cleaning and disinfection after transferring the zebrafish to ensure the plate is ready for next use, further increasing the workload of the user. To address this issue, we propose a device and method for tracking and monitoring fish stimulus-response behavior. Summary of the Invention

[0005] The purpose of the present invention is to provide a device and method for tracking and monitoring fish stimulus response behavior to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A fish stimulation response behavior tracking and monitoring device comprises an observation box and an animal behavior trajectory tracking system communicatively connected to the observation box, wherein the animal behavior trajectory tracking system is pre-configured in a virtual environment and is used to receive and analyze and process fish behavior trajectory data collected by the observation box, the observation box comprises a box body with a hollow side, a support frame is provided in the box body, a carrying plate is attached to the top of the support frame, a porous plate for placing fish is inserted on the carrying plate, the lower end of the porous plate extends into the support frame, a stimulation mechanism for stimulating fish in the porous plate is provided in the support frame, an infrared camera is provided on the inner wall of the box body above the support frame, the carrying plate is movably arranged in the box body by a driving mechanism, an auxiliary mechanism is provided on the inner wall of the top of the box body and located on the outside of the infrared camera, the auxiliary mechanism is used to collect fish located in the porous plate when the driving mechanism drives the carrying plate to move to a preset position.

[0008] A further improvement is that the stimulation mechanism includes a light stimulation unit, a sound stimulation unit and a vibration stimulation unit; it also includes a water supply pipe with one end connected to the inner cavity of the support frame and the other end connected to the external water supply equipment and a heater arranged in the support frame, and the support frame is also connected to the external pump body through a drainage pipe.

[0009] A further improvement is that the driving mechanism includes:

[0010] Two sets of movable bases are respectively located at the two ends of the bottom of the supporting plate and are slidably connected to the inner wall of the bottom of the box body. The two sets of movable bases are respectively threadedly sleeved on the outer walls of the two sets of screws. The screws are longitudinally rotated and arranged in the box body, and one end of the screws movably passes through the box body and is connected to the output end of the rotating device; and

[0011] The two sets of telescopic devices are respectively arranged at the two ends of the top of the supporting plate, and the output ends of the two sets of telescopic devices pass through the supporting plate and are respectively connected to the two sets of movable bases.

[0012] A further improvement is that the auxiliary mechanism includes:

[0013] A top plate is detachably mounted on the top inner wall of the box body, the bottom of the top plate is connected to the bottom plate via a connecting rod, a plurality of adsorption cylinders are detachably mounted on the bottom plate, the lower ends of the adsorption cylinders are connected to adsorption heads for entering the holes of the porous plate, a piston is mounted in the adsorption cylinder, and the piston passes through the top of the adsorption cylinder via a piston rod; and

[0014] The second telescopic device is arranged at the bottom of the top plate. The output end of the second telescopic device is provided with a movable plate, and the movable plate is detachably connected to the piston rod.

[0015] A further improvement is that the vertical cross-section of the porous plate is T-shaped, and a T-shaped opening for placing the porous plate is provided on the supporting plate, the length of the supporting plate is greater than the length of the supporting frame and less than the length of the inner cavity of the box cover, and box bodies are symmetrically slidably provided on both sides of the top of the supporting plate, and the opposite sides and bottoms of the two groups of box bodies are hollow, and the opposite sides of the two groups of box bodies are fitted with three output ends of a telescopic device, and the three telescopic devices are fixedly arranged on the inner wall of the box for driving the two groups of box bodies to contact, and the opposite sides of the two groups of box bodies are also connected to the top of the supporting plate by elastic members for driving the box bodies to reset, and the top inner walls of the two groups of box bodies are provided with diverter plates, and the bottom of the diverter plates are provided with several groups of nozzles, one of the diverter plates is provided with a connected docking pipe on the side close to the porous plate, and the other diverter plate is provided with a docking hole for inserting the docking pipe on the side close to the porous plate, and one of the diverter plates is connected to the external drying equipment and disinfectant supply equipment through a circulation pipeline.

[0016] A further improvement is that the inner walls of the two groups of box bodies are vertically provided with electric guide rail mechanisms, and an elastic telescopic rod is horizontally rotated on the slider of the electric guide rail mechanism, and the other end of the elastic telescopic rod is provided with a pressure plate for contacting the porous plate, and the outer wall of the fixed section of the elastic telescopic rod is provided with a gear, and a rack is provided on the guide rail of the electric guide rail mechanism, which is used to drive the gear to drive the elastic telescopic rod to rotate 180° when the slider drives the elastic telescopic rod upward to a preset position.

[0017] A further improvement is that a lens frame is provided on the inner wall of the box body through rotation of a rotator, and a lens body for covering the top of the porous plate is embedded in the lens frame.

[0018] A further improvement is that one side of the box body is provided with a box cover for closing the hollow side thereof.

[0019] A method for tracking and monitoring fish stimulus response behavior, using the above-mentioned device, comprises the following steps:

[0020] S1: During use, several zebrafish larvae that have been continuously exposed to CYN exposure solutions of different concentrations for a preset time are placed in a porous plate containing embryo culture water, the porous plate is placed on a carrier plate, and the carrier plate is driven to the support frame by a driving mechanism;

[0021] S2: stimulating the zebrafish larvae in the multi-hole plate through the stimulation mechanism and collecting the motion trajectory data of the zebrafish larvae through the infrared camera, and sending the motion trajectory data to the animal behavior trajectory tracking system for analysis and processing;

[0022] Among them, after the tracking and monitoring work is completed, when the carrying plate is moved to the preset position by the driving mechanism, the zebrafish fry located in the porous plate are sucked by the auxiliary mechanism, and then the zebrafish fry are transferred to an external placement container by the auxiliary mechanism.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1) The present invention uses a stimulation mechanism to stimulate zebrafish fry in a porous plate and uses an infrared camera to collect the motion trajectory data of the zebrafish fry and send the data to an animal behavior trajectory tracking system for analysis and processing, thereby achieving tracking and monitoring of the fish. At the same time, an auxiliary mechanism is provided. After the tracking and monitoring work is completed, the auxiliary mechanism can replace manual labor to transfer the zebrafish fry in the porous plate to a storage container, which not only saves time but also greatly reduces manual labor intensity.

[0025] 2) The present invention is also provided with a supporting plate, a box body, a telescopic device three, an elastic member, a circulation pipeline and a diverter plate and other structures, which can enable the porous plate to be disinfected and cleaned by the disinfectant liquid supplied by the disinfectant supply device in the space formed by the combination of the two sets of box bodies and dried by the drying equipment without manual processing, further reducing the labor intensity and improving the applicability of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a structural diagram of the observation box of the present invention;

[0027] Figure 2 For the present invention Figure 1 Schematic diagram of the internal structure;

[0028] Figure 3 For the present invention Figure 2 Schematic diagram of the local structure in;

[0029] Figure 4 Schematic diagram of the driving mechanism structure of the present invention;

[0030] Figure 5 It is a schematic structural diagram of the auxiliary mechanism of the present invention;

[0031] Figure 6 It is a schematic structural diagram of the electric guide rail mechanism of the present invention.

[0032] In the figure: 1. Box body; 101. Box cover; 2. Support frame; 3. Water supply pipe; 4. Load-bearing plate; 5. Perforated plate; 6. Screw; 7. Moving base; 8. Telescopic device 1; 9. Auxiliary mechanism; 91. Top plate; 92. Bottom plate; 93. Adsorption cylinder; 94. Adsorption head; 95. Movable plate; 96. Telescopic device 2; 10. Lens body; 11. Box body; 12. Telescopic device 3; 13. Elastic part; 14. Infrared camera; 15. Diverter plate; 16. Circulation pipeline; 17. Docking pipe; 18. Electric guide rail mechanism; 19. Elastic telescopic rod; 20. Rack; 21. Gear; 22. Pressure plate. DETAILED DESCRIPTION

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0034] Please refer to the attached Figure 1 - attached Figure 2

[0035] A fish stimulus response behavior tracking and monitoring device includes an observation tank and an animal behavior trajectory tracking system communicatively connected to the observation tank. Among them, the animal behavior trajectory tracking system is pre-configured in a virtual environment (such as a computer) and is used to receive and analyze the fish behavior trajectory data collected by the observation tank. The animal behavior trajectory tracking system includes a test hardware control module, a multi-observation area module, etc., which belong to the prior art and will not be elaborated here.

[0036] The observation tank includes a box body 1 with one side hollow. A box cover 101 is provided on one side of the box body 1 to close its hollow side. A support frame 2 is provided inside the box body 1. The support frame 2 is in a shape of a rectangle with a hollow center. A bearing plate 4 is attached to the top of the support frame 2. A perforated plate 5 for placing fish is inserted on the bearing plate 4. The lower end of the perforated plate 5 extends into the support frame 2. A stimulation mechanism for stimulating the fish in the perforated plate 5 is provided inside the support frame 2. Preferably, the stimulation mechanism includes a light stimulation unit, a sound stimulation unit, and a vibration stimulation unit. Among them, the light stimulation unit is, for example, an adjustable light source, such as a TTL-controlled white light source. These light sources can be automatically switched on and off and the brightness can be adjusted according to requirements, and are used to study the physiological behaviors of zebrafish such as circadian rhythm and startle response. The sound stimulation unit includes a speaker provided on the outer wall of the support frame 2, and the fish is stimulated by playing sound through the speaker. The vibration stimulation unit includes a vibrator with adjustable vibration intensity, and the fish is vibrationally stimulated by applying different vibration intensities to the perforated plate 5 through the vibrator. Of course, the above light stimulation unit, sound stimulation unit, and vibration stimulation unit are not limited to these implementation methods.

[0037] The stimulation mechanism further includes a water supply pipe 3 with one end communicating with the inner cavity of the support frame 2 and the other end communicating with an external water supply device, and a heater provided inside the support frame 2. The external water supply device can inject liquid into the support frame 2 through the water supply pipe 3. The injected liquid enters the support frame 2 and contacts the perforated plate 5. The water temperature inside the support frame 2 can be controlled by the heater to better obtain the fish behavior trajectory data. The support frame 2 is also connected to an external pump body through a drainage pipeline to drain the liquid inside the support frame 2.

[0038] An infrared camera 14 is provided on the inner wall of the box body 1 above the supporting frame 2, and the carrying plate 4 is movably arranged in the box body 1 through a driving mechanism. An auxiliary mechanism 9 is provided on the top inner wall of the box body 1 and on the outside of the infrared camera 14. The auxiliary mechanism 9 is used to collect the fish located in the porous plate 5 when the driving mechanism drives the carrying plate 4 to move to a preset position. The auxiliary mechanism 9 can assist the user in collecting and transferring the fish in the porous plate 5 after the tracking and monitoring work is completed, thereby reducing the intensity of manual labor.

[0039] Please see the attached Figure 3 -Attached Figure 4

[0040] Preferably, the driving mechanism of this embodiment includes:

[0041] Two sets of movable bases 7 are respectively located at the two ends of the bottom of the supporting plate 4 and are slidably connected to the inner wall of the bottom of the box body 1. The two sets of movable bases 7 are respectively threadedly sleeved on the outer walls of the two sets of screws 6. The screws 6 are longitudinally rotated in the box body 1, and one end of the screw 6 movably passes through the box body 1 and is connected to the output end of the rotating device (not shown in the figure). The rotating device is, for example, a servo motor and a reducer. By turning on the rotating device to drive the screws 6, the movable base 7 can drive the supporting plate 4 to move; and

[0042] The two sets of telescopic devices 8 are respectively arranged at the two ends of the top of the supporting plate 4, and the output ends of the two sets of telescopic devices 8 pass through the supporting plate 4 and are respectively connected to the two sets of movable bases 7.

[0043] During use, the supporting plate 4 is driven upward by the telescopic device 8, so that the porous plate 5 is separated from the supporting frame 2 upward, and then the movable base 7 is driven by the rotating device and the screw 6 to drive the supporting plate 4 to move to the auxiliary mechanism 9, and then the supporting plate 4 is driven upward by the telescopic device 8 to correspond to the auxiliary mechanism 9, and the fish in the porous plate 5 can be collected and transferred by the auxiliary mechanism 9.

[0044] Please see the attached Figure 5

[0045] Preferably, the auxiliary mechanism 9 of this embodiment includes:

[0046] The top plate 91 is detachably mounted on the top inner wall of the box body 1. The top plate 91 can be connected to the top inner wall of the box body 1 by a snap-fit ​​structure. The bottom of the top plate 91 is connected to the bottom plate 92 by a connecting rod. Several groups of adsorption cylinders 93 are detachably mounted on the bottom plate 92. The bottom plate 92 and the adsorption cylinders 93 can be fixed by bolts or a snap-fit ​​structure. Specifically, several groups of adsorption cylinders 93 are arranged linearly, and their number can correspond to the number of holes in a row on the porous plate 5. The lower end of the adsorption cylinder 93 is connected to an adsorption head 94 for entering the holes in the porous plate 5. A piston is provided in the adsorption cylinder 93. The piston passes through the top of the adsorption cylinder 93 through a piston rod. The piston is driven by the piston rod to move in the adsorption cylinder 93, so that the liquid and fish in the porous plate 5 can be sucked into the adsorption cylinder 93. When transferring to other containers, the liquid and fish can be discharged by pressing the piston; and

[0047] The telescopic device 2 96 is provided at the bottom of the top plate 91. The output end of the telescopic device 2 96 is provided with a movable plate 95. The movable plate 95 is detachably connected to the piston rod. The movable plate 95 and the piston rod can be connected by a snap-fit ​​structure. The movable plate 95 can be driven by the telescopic device 2 96 to drive the piston rod to move upward. It should be noted that the telescopic device 2 96 drives the movable plate 95 to move a small distance each time, that is, to extract the liquid and fish in the holes of the porous plate 5. After the liquid and fish in the holes of a row in the porous plate 5 are extracted, the rotating device and the screw 6 can continue to drive the bearing The carrier plate 4 moves so that another row of holes on the porous plate 5 corresponds to the adsorption cylinder 93. After all the fish in the porous plate 5 are extracted, the user can remove the auxiliary mechanism 9 from the box body 1 or place the placement container under the adsorption cylinder 93. By aligning the adsorption cylinder 93 with the placement container to be transferred, and then driving the movable plate 95 to drive the piston rod to move downward and reset, the liquid and fish in the adsorption cylinder 93 can be discharged into the placement container. The user can also remove the required adsorption cylinder 93 separately as needed and transfer the fish in the adsorption cylinder 93 to the required position.

[0048] The trolley case 11 is provided with a plurality of trolley cases 11, and the trolley case 11 is provided with a plurality of trolley cases 11. The trolley case 11 is provided with a plurality of trolley cases 11 and a plurality of trolley cases 11 are provided with a plurality of trolley cases 11. , the top inner walls of the two groups of box bodies 11 are both provided with diverter plates 15, and the bottom of the diverter plates 15 are provided with several groups of nozzles, one of which is provided with a connected docking pipe 17 on the side close to the porous plate 5, and the other diverter plate 15 is provided with a docking hole for inserting the docking pipe 17 on the side close to the porous plate 5. When the two groups of box bodies 11 are in contact, the docking pipe 17 of one diverter plate 15 enters the docking hole of the other diverter plate 15, and then the two diverter plates 15 are connected to each other, and one diverter plate 15 is connected to the external drying equipment and disinfectant supply equipment through a circulation pipe 16. The circulation pipe 16 may include a pipeline and a three-way pipe connector, and the two ends of the three-way pipe connector are respectively connected to the drying equipment and the disinfectant supply equipment through pipelines, and a valve body is also provided on the corresponding pipeline; the drying equipment is, for example, a heater, and the disinfectant supply equipment includes, for example, a box body for storing disinfectant liquid and a pump body for inputting the disinfectant liquid in the box body into the circulation pipe 16;

[0049] After the fish and liquid in the porous plate 5 are collected by the auxiliary mechanism 9, the porous plate 5 is driven to return to the support frame 2 by the rotating device and the screw 6, and the two groups of box bodies 11 are driven to contact each other by the telescopic device 3 12, and then the disinfectant liquid is supplied by the disinfectant supply device. The disinfectant liquid enters the diverter plate 15 and is then sprayed out from the nozzle to clean and disinfect the porous plate 5. After disinfection, an air flow with a certain amount of heat can be supplied by the drying device to dry the porous plate 5.

[0050] Please see the attached Figure 6

[0051] Preferably, the inner walls of the two groups of box bodies 11 of this embodiment are vertically provided with an electric guide rail mechanism 18, which belongs to the prior art and includes structures such as guide rails, sliders, and electric motors. An elastic telescopic rod 19 is provided on the slider of the electric guide rail mechanism 18 for horizontal rotation, and a pressure plate 22 for contacting the porous plate 5 is provided at the other end of the elastic telescopic rod 19. The cross section of the pressure plate 22 can be T-shaped, and a groove for the pressure plate 22 to enter can be provided on the side wall of the porous plate 5. In the process of the two groups of box bodies 11 approaching each other, the elastic telescopic rod 19 is pressed so that the pressure plate 22 is in close contact with the porous plate 5. The outer wall of the fixed section of the elastic telescopic rod 19 is provided with a gear 21. A rack 20 is provided on the guide rail of the rail mechanism 18, which is used to drive the gear 21 to drive the elastic telescopic rod 19 to rotate 180° when the slider drives the elastic telescopic rod 19 upward to a preset position. After the two groups of box bodies 11 contact each other, the electric guide rail mechanism 18 drives the slider to move upward, and then drives the porous plate 5 upward to separate from the supporting plate 4 through the elastic telescopic rod 19 and the pressure plate 22. In the upward process, the gear 21 and the rack 20 cooperate so that the elastic telescopic rod 19 and the pressure plate 22 drive the porous plate 5 to rotate 180°, which not only allows the disinfectant liquid in the porous plate 5 to separate from the porous plate 5 and enter the support frame 2, but also allows the porous plate 5 to be fully disinfected and dried.

[0052] Preferably, the inner wall of the box body 1 of this embodiment is provided with a lens frame which is rotated by a rotator, and the rotator is, for example, a rotary motor. A lens body 10 for covering the top of the porous plate 5 is embedded in the lens frame. The lens frame is driven to rotate to a horizontal state by the rotator, so that the lens body 10 can correspond to and fit on the top of the porous plate 5, and the infrared camera 14 can better track and monitor fish through the lens body 10.

[0053] A method for tracking and monitoring fish stimulus response behavior, using the above-mentioned device, comprises the following steps:

[0054] S1: During use, several zebrafish larvae that have been continuously exposed to CYN exposure solutions of different concentrations for a preset time are placed in a porous plate 5 containing embryo culture water, the porous plate 5 is placed on a carrier plate 4, and the carrier plate 4 is driven to the support frame 2 by a driving mechanism;

[0055] S2: stimulating the zebrafish fry in the porous plate 5 by the stimulation mechanism and collecting the motion trajectory data of the zebrafish fry by the infrared camera 14 and sending the motion trajectory data to the animal behavior trajectory tracking system for analysis and processing;

[0056] Among them, after the tracking and monitoring work is completed, when the carrying plate 4 is moved to the preset position by the driving mechanism, the zebrafish fry located in the porous plate 5 is sucked by the auxiliary mechanism 9, and then the zebrafish fry are transferred to an external placement container by the auxiliary mechanism 9. After the zebrafish fry are transferred to the external placement container, the carrying plate 4 is driven by the driving mechanism to be reset to the support frame 2, and then the two groups of box bodies 11 are driven to contact each other by the telescopic device 3 12, and then the disinfectant liquid is supplied by the disinfectant supply device, and the disinfectant liquid enters the diverter plate 15 and is then sprayed out from the nozzle to clean and disinfect the porous plate 5. After disinfection, an air flow with a certain amount of heat is supplied by the drying device to dry the porous plate 5.

[0057] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A fish stimulus response behavior tracking and monitoring device, comprising an observation box and an animal behavior trajectory tracking system connected to the observation box, wherein: The animal behavior trajectory tracking system is pre-configured in a virtual environment and is used to receive and analyze the fish behavior trajectory data collected by the observation box, and is characterized in that: the observation box includes a box body (1) with a hollow side, a support frame (2) is provided in the box body (1), a carrying plate (4) is attached to the top of the support frame (2), a porous plate (5) for placing fish is inserted on the carrying plate (4), the lower end of the porous plate (5) extends into the support frame (2), and a stimulation mechanism for stimulating the fish in the porous plate (5) is provided in the support frame (2), an infrared camera (14) is provided on the inner wall of the box body (1) above the support frame (2), the carrying plate (4) is movably arranged in the box body (1) by a driving mechanism, and an auxiliary mechanism (9) is provided on the inner wall of the top of the box body (1) and located outside the infrared camera (14), and the auxiliary mechanism (9) is used to collect the fish in the porous plate (5) when the driving mechanism drives the carrying plate (4) to move to a preset position; The auxiliary mechanism (9) comprises: A top plate (91) is detachably mounted on the top inner wall of the box body (1); the bottom of the top plate (91) is connected to a bottom plate (92) via a connecting rod; a plurality of adsorption cylinders (93) are detachably mounted on the bottom plate (92); the lower ends of the adsorption cylinders (93) are connected to adsorption heads (94) for entering the holes of the porous plate (5); a piston is mounted in the adsorption cylinder (93); and the piston penetrates the top end of the adsorption cylinder (93) via a piston rod; and The second telescopic device (96) is arranged at the bottom of the top plate (91). The output end of the second telescopic device (96) is provided with a movable plate (95), and the movable plate (95) is detachably connected to the piston rod.

2. The device according to claim 1, characterized in that: The stimulation mechanism comprises a light stimulation unit, a sound stimulation unit and a vibration stimulation unit; and further comprises a water supply pipe (3) having one end connected to the inner cavity of the support frame (2) and the other end connected to an external water supply device, and a heater arranged in the support frame (2); the support frame (2) is also connected to an external pump body via a drainage pipe.

3. The device according to claim 1, characterized in that: The driving mechanism comprises: Two groups of movable bases (7) are respectively located at the two ends of the bottom of the supporting plate (4) and are slidably connected to the inner wall of the bottom of the box (1). The two groups of movable bases (7) are respectively threadedly sleeved on the outer walls of the two groups of screw rods (6). The screw rods (6) are longitudinally rotated and arranged in the box (1), and one end of the screw rod (6) is movable through the box (1) and is connected to the output end of the rotating device; and Two sets of telescopic devices (8) are respectively arranged at the two ends of the top of the supporting plate (4), and the output ends of the two sets of telescopic devices (8) pass through the supporting plate (4) and are respectively connected to the two sets of movable bases (7).

4. The device according to claim 1, characterized in that: The vertical cross section of the porous plate (5) is T-shaped, and a T-shaped opening for placing the porous plate (5) is provided on the supporting plate (4). The length of the supporting plate (4) is greater than the length of the supporting frame (2) and less than the length of the inner cavity of the box cover (101). Box bodies (11) are symmetrically slidably provided on both sides of the top of the supporting plate (4). The opposite sides and the bottoms of the two groups of the box bodies (11) are hollow. The opposite sides of the two groups of the box bodies (11) are both fitted with the output end of a telescopic device (12). The telescopic device (12) is fixedly provided on the inner wall of the box body (1) and is used to drive the two groups of the box bodies (11) to contact each other. The opposite side of the box body (11) is also connected to the top of the carrier plate (4) through an elastic member (13) for driving the box body (11) to reset. The top inner walls of the two groups of box bodies (11) are both provided with a diverter plate (15). The bottom of the diverter plate (15) is provided with a plurality of nozzles. One of the diverter plates (15) is provided with a connected butt joint pipe (17) on the side close to the porous plate (5), and the other diverter plate (15) is provided with a butt joint hole for inserting the butt joint pipe (17) on the side close to the porous plate (5). One of the diverter plates (15) is connected to an external drying device and a disinfectant supply device through a circulation pipeline (16).

5. The device according to claim 4, characterized in that: The inner walls of the two groups of box bodies (11) are both vertically provided with electric guide rail mechanisms (18), and an elastic telescopic rod (19) is horizontally rotatably provided on the slider of the electric guide rail mechanism (18), and a pressure plate (22) for contacting the porous plate (5) is provided at the other end of the elastic telescopic rod (19), and a gear (21) is sleeved on the outer wall of the fixed section of the elastic telescopic rod (19). A rack (20) is provided on the guide rail of the electric guide rail mechanism (18), which is used to drive the gear (21) to drive the elastic telescopic rod (19) to rotate 180 degrees when the slider drives the elastic telescopic rod (19) upward to a preset position.

6. The device according to claim 1, characterized in that: A lens frame is provided on the inner wall of the box body (1) through rotation of a rotator, and a lens body (10) for covering the top of the porous plate (5) is embedded in the lens frame.

7. The device according to claim 1, characterized in that: One side of the box body (1) is provided with a box cover (101) for closing the hollow side thereof.

8. A method for tracking and monitoring fish stimulus response behavior, utilizing the device according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1: When in use, a plurality of zebrafish larvae that have been continuously exposed to CYN exposure solutions of different concentrations for a preset time are placed in a porous plate (5) containing embryo culture water, the porous plate (5) is placed on a carrier plate (4), and the carrier plate (4) is driven to the support frame (2) by a driving mechanism; S2: stimulating the zebrafish larvae in the porous plate (5) through the stimulation mechanism and collecting the motion trajectory data of the zebrafish larvae through the infrared camera (14) and sending the motion trajectory data to the animal behavior trajectory tracking system for analysis and processing; After the tracking and monitoring work is completed, when the carrying plate (4) is driven by the driving mechanism to move to a preset position, the zebrafish fry located in the porous plate (5) is sucked by the auxiliary mechanism (9), and then the zebrafish fry is transferred to an external placement container by the auxiliary mechanism (9).

Images