Bactericidal composition containing mandipropamid and application thereof

Abstract

The present invention belongs to the technical field of pesticide sterilization, and discloses a mandipropamid-containing sterilization composition, the sterilization composition comprises an active component A and an active component B, the active component A is mandipropamid, the active component B is any one of fluoxapiprolin or flufenoxadiazam, and the mass ratio of the active component A to the active component B is 1: 50-30: 1. The bactericidal composition disclosed by the invention has a remarkable synergistic effect on diseases caused by plant oomycetes pathogenic bacteria, the application amount and the application frequency of chemical pesticides are reduced, the application efficiency is improved, the agricultural production cost is reduced, the generation and development of drug resistance of pathogenic bacteria are slowed down, and pesticide residues are reduced; the agricultural product food safety is guaranteed while the farmland ecological environment is protected.

Description

[0001] This invention application is a divisional application with application number CN202310302932.0, application date March 27, 2023, and invention name “A fungicidal composition containing dimethomorph and its use”. Technical Field

[0002] The invention belongs to the technical field of pesticide sterilization, and specifically discloses a sterilization composition containing mandipropamid and application thereof. Background Art

[0003] Mandelic acid amide is a new type of oomycete fungicide developed by Syngenta. It is a mandelic acid amide compound. Its chemical name is 2-(4-chlorophenyl)-N-[2-(3-methoxy-4-prop-2-ynyloxy-phenyl)-ethyl]-2-prop-2-ynyloxy-acetamide, and its CAS number is 374726-62-2. Mandelic acid amide can effectively inhibit spore germination, and has a high affinity for the wax layer on the surface of plants, and has excellent resistance to rain erosion. Mandelic acid amide can also penetrate into plant tissues and inhibit the growth of mycelium. It is an efficient fungicide for the prevention and control of downy mildew and late blight on various crops. It has the characteristics of preventing, treating and reducing the number of pathogens.

[0004] Oomycetes belong to the Oomycetes of the Pseudomycetes kingdom and are an important plant disease. The more serious diseases include Pythium, Downy Mildew, Phytophthora, and White Spotted Fungus, which cause plant damping-off, fruit rot, late blight of potatoes and tomatoes, downy mildew of grapes, and white rust of the cruciferous plant. Oomycetes are highly destructive and harmful to host plants, and most plant diseases caused by oomycetes are difficult to control. In particular, downy mildew and Phytophthora have short incubation periods and many reinfections, and often break out and cause serious losses.

[0005] In order to effectively control the harm of plant pathogens, chemical control has always been the main method of agricultural production. However, the long-term and large-dose use of a single agent can easily cause pathogens to develop drug resistance, thereby failing to achieve the desired control effect. Therefore, the use of compound agents to screen out efficient and economical compound agents is of great significance to the healthy development of agriculture. The inventors conducted a large number of experimental studies and found that compounding dimethomorph with any one of fluoxapiprolin or flufenoxadiazam has a significant synergistic effect on a variety of oomycete pathogens in plants within a suitable ratio range, reducing the dosage of the agent while reducing the agent residues in agricultural products, thereby ensuring the food safety of agricultural products. Summary of the invention

[0006] Based on the above situation, the purpose of the present invention is to provide a fungicide composition containing mandevaccin and its preparation, and provide the use of the fungicide composition for preventing and treating oomycete plant diseases. The fungicide composition has a significant synergistic effect, can effectively control the resistance of pathogenic bacteria, reduce the application amount and application frequency of chemical pesticides, improve application efficiency, reduce the cost of agricultural production, reduce pesticide residues, protect the ecological environment of farmland, and reduce the drug residues of agricultural products.

[0007] In order to achieve the above-mentioned object, the present invention adopts the following technical scheme: a fungicide composition containing mandipropamid, wherein the fungicide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is mandipropamid, and the active ingredient B is any one of fluoxapiprolin or flufenoxadiazam, and the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 30:1.

[0008] Furthermore, the active ingredient B is fluoxapiprolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 20:1; the active ingredient B is flufenoxadiazam, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 10:1.

[0009] Furthermore, the active ingredient B is fluoxapiprolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 10:1;

[0010] The active ingredient B is flufenoxadiazam, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 5:1.

[0011] Furthermore, the total weight of the bactericidal composition is 100%wt, and the total weight of the active ingredient A and the active ingredient B accounts for 5% to 70% of the total weight of the bactericidal composition.

[0012] Furthermore, the fungicidal composition includes, in addition to the active ingredient, an agriculturally acceptable auxiliary ingredient, wherein the auxiliary ingredient is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier;

[0013] Furthermore, the wetting agent is selected from a mixture of one or more of sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, lakai powder BX, wetting and penetrating agent F, sapodilla powder, silkworm feces or soapberry powder;

[0014] Further, the dispersant is selected from a mixture of one or more of polycarboxylates, lignin sulfonates, alkylphenol polyoxyethylene ether formaldehyde condensate sulfates, alkylbenzene sulfonate calcium, naphthalenesulfonic acid formaldehyde condensate sodium salts, alkylphenol polyoxyethylene ethers, fatty amine polyoxyethylene ethers, fatty acid polyoxyethylene ethers or glycerol fatty acid ester polyoxyethylene ethers;

[0015] Further, the emulsifier is selected from calcium alkylbenzene sulfonate, OP series phosphate (nonylphenol polyoxyethylene ether phosphate), phenylphenol polyoxyethylene ether phosphate, styrene polyoxyethylene ether ammonium sulfate, alkyl biphenyl ether disulfonic acid magnesium salt, triethanolamine salt, benzyl dimethylphenol polyoxyethyl ether, alkylphenol formaldehyde resin polyoxyethyl ether, phenethylphenol formaldehyde resin polyoxyethyl ether, phenethylphenol polyoxyethyl polypropylene ether, ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), castor oil polyoxyethylene ether, alkylaryl polyoxyethylene polyoxypropylene ether, sorbitan monostearate, sorbitan fatty acid ester polyoxyethylene ether or fatty alcohol polyoxyethylene ether.

[0016] Furthermore, the thickener is selected from a mixture of one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;

[0017] Furthermore, the disintegrant is selected from a mixture of one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;

[0018] Furthermore, the antifreeze agent is selected from a mixture of one or more of ethylene glycol, propylene glycol, glycerol or urea;

[0019] Furthermore, the defoaming agent is selected from silicone oil, silicone compounds, C 10 ~C 20 Saturated fatty acid compounds or C8~C 10 A mixture of one or more fatty alcohol compounds;

[0020] Further, the solvent is selected from a mixture of one or more of N,N-dimethylformamide, cyclohexanone, butyl ether, xylene, dimethyl sulfoxide, methanol, ethylene glycol, ethanol, propanol, butanol, trimethylcyclohexanone, N-octyl pyrrolidone, toluene, ethanolamine, triethanolamine, isopropylamine, N-methyl pyrrolidone, diethylene glycol, ethylene glycol methyl ether, ethyl acetate or acetonitrile;

[0021] Furthermore, the stabilizer is selected from a mixture of one or more of epoxy soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0022] Further, the penetrant is selected from a mixture of one or more of penetrant JFC, penetrant T, azone or silicone;

[0023] Furthermore, the carrier is one, two or three of a solvent or a filler, and water is preferably deionized water;

[0024] Furthermore, the filler is selected from a mixture of one or more of kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

[0025] The above substances can all be obtained commercially;

[0026] The bactericidal composition of the present invention can be prepared into any formulation form permitted in agriculture as required, and the formulation form is selected from a solid formulation or a liquid formulation;

[0027] The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0028] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions or microcapsule suspension-suspoemulsions;

[0029] Further, the solid preparation is selected from wettable powder or water dispersible granules, and the liquid preparation is selected from suspension;

[0030] The invention also discloses the use of the bactericidal composition for preventing and controlling plant oomycete diseases.

[0031] Furthermore, the Oomycete diseases are diseases caused by the following pathogenic bacteria: pathogenic bacteria of the Pythiaceae family, pathogenic bacteria of the Peronophythoraceae family, pathogenic bacteria of the Peronosporaceae family, and pathogenic bacteria of the Albuginaceae family.

[0032] Further, the pathogenic bacteria of the Pythiaceae family are Trachysphaera, Phytophthora, Diasparangium, Phythiogeton, and Scleriphthora; the pathogenic bacteria of the Peronophythoraceae family are Peronophythoraceae litchii; the pathogenic bacteria of the Peronosporaceae family are Basidiophora, Sclerospora, Peronosclerospora, Bremia, Bremiella, Paraperonospora, Plasmopara, Peronospora, or Pseudopoeronospora;

[0033] Furthermore, the Oomycete disease is caused by the following pathogenic bacteria: pathogenic bacteria of the genus Phytophthora or pathogenic bacteria of the genus Peronospora;

[0034] Furthermore, the bactericidal composition or its preparation is applied to the pathogens to be controlled or their growth medium.

[0035] The present invention has the following beneficial effects:

[0036] The bactericidal composition of the present invention has obvious synergistic effects on Oomycete pathogens, enhances bactericidal activity, reduces the application amount and application frequency of chemical pesticides, improves application efficiency, reduces agricultural production costs, slows down the generation and development of pathogen resistance, reduces pesticide residues in agricultural products, protects the ecological environment of farmland, and ensures the food safety of agricultural products. DETAILED DESCRIPTION

[0037] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0038] Preparation Examples: The percentages in the preparation examples are all by weight.

[0039] Preparation Example 1: 20% mandipropamid·fluoxapiprolin suspension (1:1)

[0040] Formula: 10% mandelin, 10% fluoxapiprolin, 2% sodium lignin sulfonate, 2% naphthalene sulfonate formaldehyde condensate, 3% fatty alcohol polyoxyethylene ether phosphate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 4% propylene glycol, 0.01% potassium benzisothiazolinone, 0.5% silicone oil, and deionized water to make up the balance;

[0041] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, water is added to mix evenly, high-speed shearing, wet sand grinding, and finally homogenized filtration to obtain the suspension product.

[0042] Preparation Example 2: 24% mandipropamid·flufenoxadiazam suspension (1:5)

[0043] Formula: 4% mandelin, 20% flufenoxadiazam, 2% isomeric tridecanol polyoxyethylene ether, 1% sodium salt of polycarboxylic acid, 3% styrenephenol polyoxyethylene ether phosphate, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance.

[0044] Preparation method: Same as Preparation Example 1.

[0045] Preparation Example 3: 35% mandipropamid·fluoxapiprolin water dispersible granules (1:4)

[0046] Formula: 7% mandelin, 28% fluoxapiprolin, 8% lignin sulfonate, 5% naphthalene sulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 5% white carbon black, 30% starch, and kaolin makes up the balance.

[0047] Preparation method: according to the formula ratio, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% of water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and sieving; or the crushed powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0048] Preparation Example 4: 44% dimethomorph·flufenoxadiazam water dispersible granules (1:10)

[0049] Formula: 4% mandelin, 40% flufenoxadiazam, 9% sodium lignin sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% sodium polycarboxylate, 2% sodium lauryl sulfate, 10% ammonium sulfate, and starch makes up the balance.

[0050] Preparation method: Same as Preparation Example 3.

[0051] Preparation Example 5: 36% mandelin-fluoxapiprolin wettable powder (1:3)

[0052] Formula: 9% mandelin, 27% fluoxapiprolin, 8% sodium lignin sulfonate, 6% sodium dodecylbenzene sulfonate, 2% BX powder, 5% white carbon black, and kaolin makes up the balance.

[0053] Preparation method: The active ingredients, dispersants, wetting agents and fillers are mixed according to the formula ratio, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly for multiple times by a jet mill to prepare the wettable powder of the composition of the present invention.

[0054] Preparation Example 6: 40% mandipropamid·flufenoxadiazam wettable powder (3:1)

[0055] Formula: 30% mandelin, 10% flufenoxadiazam, 4% sodium lignin sulfonate, 3% sodium polycarboxylate, 3% dispersant NNO, 2% fatty alcohol polyoxyethylene ether, 5% white carbon black, and kaolin makes up the balance.

[0056] Preparation method: Same as Preparation Example 5.

[0057] Indoor activity test

[0058] Example 1: Indoor activity test of different agents against cucumber downy mildew

[0059] Test basis: The test refers to NY / T 1156.7-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 7: Pot Method for Control of Cucumber Downy Mildew".

[0060] Test target: Cucumber downy mildew (Pseudoperonospora cubensis).

[0061] Test instruments and equipment: electronic balance, spray equipment, artificial climate chamber, biological incubator, culture dish, pipette, etc.

[0062] Test material preparation: Select disease-susceptible cucumber varieties for potted plants (Xintai Mici), and keep the seedlings ready when they grow to 4 to 6 true leaves.

[0063] Test drugs: fluoxapiprolin technical, flufenoxadiazam technical, and mandipropamid technical, provided by the Group's R&D Center.

[0064] Test steps:

[0065] (1) Preparation of sporangium suspension

[0066] Select the infected cucumber leaves, wash the spores of downy mildew on the back of the leaves with 4°C distilled water, and prepare a suspension (concentration of 1×10 5 ~1×10 7 The samples were stored at 4°C for later use.

[0067] (2) Preparation of medicines

[0068] Dissolve the original drug in a suitable solvent and then dilute it with 0.1% Tween 80 aqueous solution. Set 5 series of mass concentrations according to the activity of the drug.

[0069] (3) Chemical treatment

[0070] According to the experimental design, each treatment agent was evenly sprayed on both sides of the leaves until they were completely wet, and the solution was allowed to dry naturally before use. The experiment set up a treatment without agent as a blank control.

[0071] (4) Inoculation and culture

[0072] Fresh sporangium suspension was sprayed and inoculated on the back of leaves, with 5 pots per treatment, 2 plants per pot, and 4 replicates per treatment. After inoculation, the plants were cultured under the conditions of light:dark = 12h:12h, temperature 17-22°C, and relative humidity 92%-95%.

[0073] Experimental investigation:

[0074] According to the disease situation of the blank control, the inoculated leaves were graded and investigated. Thirty leaves were investigated for each treatment, and the following grading method was used for the graded investigation and recorded:

[0075] Level 0: no disease;

[0076] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0077] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0078] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0079] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0080] Level 9: The lesion area accounts for more than 50% of the entire leaf area;

[0081] Data calculation:

[0082] Based on the data investigation, the disease index and prevention and control effect of each treatment were calculated.

[0083] The disease index was calculated according to the following formula:

[0084]

[0085] The control effect is calculated according to the following formula:

[0086]

[0087] Statistical analysis:

[0088] Use IBM SPSS Statistics statistical analysis system to analyze and find EC 50 The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0089]

[0090] Where:

[0091] ATI——Actual Toxicity Index of Mixture;

[0092] S——EC of standard medicine 50 , the unit is milligrams per liter (mg / L);

[0093] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0094] TTI=TI A ×P A +TI B ×P B

[0095] Where:

[0096] TTI – Theoretical Toxicity Index of Mixtures;

[0097] TI A ——A: toxicity index of the agent;

[0098] P A ——The percentage of agent A in the mixture, in percentage (%);

[0099] TI B ——Toxicity index of agent B;

[0100] P B ——The percentage of agent B in the mixture, in percentage (%).

[0101]

[0102] Where:

[0103] CTC – Co-toxicity coefficient;

[0104] ATI——Actual Toxicity Index of Mixture;

[0105] TTI - Theoretical Toxicity Index of Mixture.

[0106] The co-toxicity coefficient of the combination CTC ≥ 120 shows a synergistic effect; CTC ≤ 80 shows an antagonistic effect; 80 < CTC < 120 shows an additive effect.

[0107] The indoor test results are shown in the following table:

[0108] Table 1 Results of indoor activity test of mandeoxypyrim and fluoxapiprolin against cucumber downy mildew

[0109] Treatment <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC mandipropamid (A) 0.590 100.000 - - fluoxapiprolin (B) 2.280 25.877 - - A + B (1:50) 2.112 27.936 27.685 102.214 A + B (1:35) 1.754 33.637 27.936 120.408 A + B (1:20) 1.496 39.439 28.268 134.113 A + B (1:10) 1.111 53.105 32.616 162.822 A + B (1:8) 1.017 58.014 34.113 170.063 A + B (1:4) 0.801 73.658 40.702 180.970 A + B (1:1) 0.529 111.531 62.939 177.206 A + B (4:1) 0.458 128.821 85.175 151.242 A + B (8:1) 0.433 136.259 91.764 148.488 A + B (10:1) 0.463 127.430 93.262 136.637 A + B (20:1) 0.491 120.163 96.470 124.559 A + B (30:1) 0.510 115.686 97.609 118.520

[0110] From the indoor test results in Table 1, it can be seen that mandelin and fluoxapiprolin have high toxicity to cucumber downy mildew. 50 The mass ratio of mandipropamid to fluoxapiprolin is 1:50-30:1, and the co-toxicity coefficient is greater than 80, and the combined effect is additive or synergistic; the mass ratio of mandipropamid to fluoxapiprolin is 1:35-20:1, and the co-toxicity coefficient is greater than 120, and the combined effect is synergistic.

[0111] Table 2 Results of indoor activity test of mandipropamid and flufenoxadiazam against cucumber downy mildew

[0112]

[0113]

[0114] It can be seen from the indoor test results in Table 2 that the mass ratio of mandipropamid to flufenoxadiazam is 1:40-30:1, the co-toxicity coefficient is greater than 80, and the combined effect is additive or synergistic; the mass ratio of mandipropamid to flufenoxadiazam is 1:30-10:1, the co-toxicity coefficient is greater than 120, and the combined effect is synergistic.

[0115] Example 2: Indoor activity test of different drug treatments on Phytophthora infestans

[0116] Test basis: The test was conducted in accordance with NY / T 1156.12-2008 “Guidelines for Indoor Bioassay Tests of Pesticides – Fungicides – Part 12: Pot Method for Controlling Late Blight”.

[0117] Test instruments: electronic balance, spray equipment, artificial climate chamber, pipette, etc.

[0118] Test reagents: acetone (analytical grade), Tween 80 (chemical grade), and distilled water.

[0119] Experimental drugs: fluoxapiprolin technical and mandipropamid technical, provided by the R&D Center of Hailier Pharmaceutical Group.

[0120] Biological test materials: The test fungus was the strain of Phytophthora infestans. The test crop was a tomato variety susceptible to late blight, which was potted and cultured to the stage of 2 to 4 true leaves and numbered for future use.

[0121] Test steps:

[0122] (1) Preparation of zoospore suspension

[0123] Collect tissues of tomatoes infected with late blight and moisturize them for culture. After sporangia are produced, wash them with sterile water and filter them with double-layer gauze to prepare a sporangium suspension. Place it in the dark at 4°C for 0.5 h to 3 h to release zoospores. Adjust the spore concentration to 1 × 10 5 The spores / mL suspension was used as inoculum and set aside.

[0124] (2) Preparation of medicines

[0125] The above original drugs were dissolved in a suitable solvent and then diluted with a 0.1% Tween 80 aqueous solution. Five series of mass concentrations were set according to the activity of the drug.

[0126] (3) Chemical treatment

[0127] Spray the agent evenly on the leaves until they are completely wet, and wait for the solution to dry naturally before use. Three pots were used for each treatment, and each treatment was repeated 4 times. A treatment containing only solvent and surfactant but no active ingredient was set as a blank control.

[0128] (4) Inoculation and culture

[0129] Spray inoculation with zoospore suspension, alternate light / darkness for 12 hours each day (light intensity 5000Lux-20000Lux) and temperature 18°C-20°C after inoculation, keep water film on leaf surface within 24 hours after inoculation, and then culture for 7 days under the condition of relative humidity above 90%.

[0130] (5) Investigation: When the diseased leaf rate of the blank control reaches more than 50%, investigate the disease status of each treatment in a graded manner. 30 leaves are investigated for each treatment. The grading method is:

[0131] Level 0: no disease;

[0132] Level 1: There are only a few small lesions on the leaves, and the lesions occupy less than 10% of the leaf area;

[0133] Level 3: The lesions on the leaves occupy 10% to 25% of the leaf area;

[0134] Level 5: The lesions on the leaves account for 26% to 50% of the leaf area;

[0135] Level 7: The lesions on the leaves cover more than 50% of the leaf area;

[0136] Level 9: All leaves become diseased and wilt.

[0137] Data calculation:

[0138] Based on the data investigation, the disease index and prevention and control effect of each treatment were calculated.

[0139] The disease index was calculated according to the following formula:

[0140]

[0141] The control effect is calculated according to the following formula:

[0142]

[0143] Statistical analysis:

[0144] Use IBM SPSS Statistics statistical analysis system to analyze and find EC 50 The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0145]

[0146] Where:

[0147] ATI——Actual Toxicity Index of Mixture;

[0148] S——EC of standard medicine 50 , the unit is milligrams per liter (mg / L);

[0149] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0150] TTI=TI A ×P A +TI B ×P B

[0151] Where:

[0152] TTI – Theoretical Toxicity Index of Mixtures;

[0153] TI A ——A: toxicity index of the agent;

[0154] P A ——The percentage of agent A in the mixture, in percentage (%);

[0155] TI B ——Toxicity index of agent B;

[0156] P B ——The percentage of agent B in the mixture, in percentage (%).

[0157]

[0158] Where:

[0159] CTC – Co-toxicity coefficient;

[0160] ATI——Actual Toxicity Index of Mixture;

[0161] TTI - Theoretical Toxicity Index of Mixture.

[0162] The co-toxicity coefficient of the combination CTC ≥ 120 shows a synergistic effect; CTC ≤ 80 shows an antagonistic effect; 80 < CTC < 120 shows an additive effect.

[0163] The indoor test results are shown in the following table:

[0164] Table 3 Results of indoor activity test of mandelin and fluoxapiprolin against Phytophthora infestans

[0165] Treatment <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC mandipropamid (A) 0.715 22.378 - - fluoxapiprolin (B) 0.160 100.000 - - A + B (1:48) 0.130 123.077 98.416 125.058 A + B (1:35) 0.119 134.454 97.844 137.417 A + B (1:24) 0.105 152.381 96.895 157.264 A + B (1:12) 0.101 158.416 94.029 168.475 A + B (1:6) 0.100 160.000 88.911 179.955 A + B (1:3) 0.106 150.943 80.594 187.288 A + B (1:1) 0.133 120.301 61.189 196.606 A + B (3:1) 0.192 83.333 41.783 199.442 A + B (6:1) 0.281 56.940 33.467 170.139 A + B (12:1) 0.342 46.784 28.349 165.030 A + B (24:1) 0.443 36.117 25.483 141.734 A + B (30:1) 0.488 32.787 24.882 131.772

[0166] From the indoor test results in Table 3, it can be seen that fluoxapiprolin and dimethomorph have high toxicity to Phytophthora infestans. The mass ratio of dimethomorph to fluoxapiprolin is 1:48-30:1, and the co-toxicity coefficient is greater than 120, and the combined effect is synergistic.

[0167] Field efficacy test

[0168] Example 3: Field test on prevention and control of cucumber downy mildew

[0169] The test was carried out with reference to GB / T 17980.26-2000 "Guidelines for field efficacy tests (I) Fungicides for the control of cucumber downy mildew".

[0170] Test object: Cucumber downy mildew (Pseudoperonospora cubensis).

[0171] Experimental crop: cucumber (Jinyou 35).

[0172] Experimental environmental conditions: The experiment was conducted in a greenhouse in Xintai City, Shandong Province. Cucumber downy mildew has occurred seriously in the experimental site over the years. The cultivation conditions in all plots are uniform and consistent, and the management level of the experimental site is good, which is in line with local scientific agricultural practices.

[0173] Experimental method: The experiment adopted a randomized block design, with each plot covering an area of ​​20m 2 , each treatment was repeated 4 times.

[0174] Experimental implementation: The first application of pesticide was carried out on May 14, 2018, at the early stage of cucumber downy mildew. The pesticide was applied once every 7 days, for a total of 2 applications. When applying pesticides, a Gongnong-16 backpack sprayer was used to spray the whole cucumber plant evenly, and the spray amount was until the front and back of the leaves were evenly wetted and the pesticide began to drip.

[0175] Investigation and efficacy calculation method: The test was conducted 7 days after the first application and 10 days after the last application. Four points were randomly selected from each plot for investigation, two plants were investigated at each point, and all leaves of each plant were investigated. The investigation was graded according to the following grading method:

[0176] Level 0: no lesions;

[0177] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0178] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0179] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0180] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0181] Level 9: The lesion area accounts for more than 50% of the total leaf area.

[0182] The efficacy is calculated according to the following formula:

[0183]

[0184]

[0185] Safety investigation: During the trial, the safety of various doses of the test agent on cucumbers was investigated, and no phytotoxicity was found.

[0186] The test results are shown in the following table:

[0187] Table 5 Results of field efficacy tests of different pesticides against cucumber downy mildew

[0188]

[0189] It can be seen from the test results in the above table that the test agent has a good control effect on cucumber downy mildew, and the test agent has no phytotoxicity to cucumber. Example 4: Field control of tomato late blight test

[0190] The test was conducted with reference to GB / T 17980.31-2000 “Guidelines for field efficacy tests (I) Fungicides for the control of early blight and late blight of tomatoes”.

[0191] Test subject: Phytophthora infestans.

[0192] Experimental crop: Tomato (Jinpeng No. 8).

[0193] The experiment was conducted in a solar greenhouse in Houhan Village, Zoucheng City, Shandong Province. The experimental land had high fertility and late blight had occurred every year.

[0194] Experimental design: The experiment set up 6 treatments, each treatment was repeated 4 times, and the plot area was 20m 2 , randomized block arrangement.

[0195] The experiment started with conventional spraying when tomato late blight occurred sporadically. The liquid was evenly sprayed on the front and back of the stems and leaves of the tomato plants so that the whole plant was covered with the liquid. The liquid application rate was 675L / hm 2 The experiment was conducted 3 times with an interval of 7 days. The application time was March 6, March 13, and March 20, 2018. The weather was sunny or cloudy during the application, and the whole experiment was not affected by bad weather.

[0196] Survey method: The control effect survey was conducted 7 days after the last application of pesticides. Five random sampling points were selected in each plot, and 2 plants were selected at each point. Ten leaves of each plant were surveyed in the upper, middle and lower parts. The grading was based on the percentage of the lesion area on each leaf to the entire leaf area. The grading method was as follows:

[0197] Level 0: no lesions;

[0198] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0199] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0200] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0201] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0202] Level 9: The lesion area accounts for more than 50% of the total leaf area.

[0203] The efficacy is calculated according to the following formula:

[0204]

[0205]

[0206] Visual inspection was used to observe the growth and leaf color of the test crops after application of the pesticide, and to examine the safety of the test pesticide on tomatoes.

[0207] During the entire test process, tomatoes grew normally and no pesticide damage was found to the tomato plants.

[0208] Results and analysis:

[0209] Table 6 Field efficacy test results of different pesticide treatments on tomato late blight

[0210]

[0211] The test results in Table 6 show that the test agent has a good control effect on tomato late blight. 7 days after the last application, the control effect of the test agent on tomato late blight was higher than 90%.

[0212] Example 5: Field test for controlling potato late blight

[0213] Test basis: The test refers to GB / T 17980.34-2000 "Guidelines for field efficacy tests (I) Fungicides for the control of potato late blight".

[0214] Test subject: Phytophthora infestans.

[0215] Experimental crop: Potato (Ji Zhang Shu No. 12).

[0216] The experiment was conducted in a potato field in Shangchuan Village, Shiquan Township, Anding District, Dingxi City, Gansu Province. The fertility of the experimental land was above average.

[0217] Experimental design: The experiment set up 6 treatments, each treatment was repeated 4 times, and the plots were arranged in random blocks, with an area of ​​35m 2 .

[0218] Application time: The test was carried out on August 1 and August 8, 2018, a total of 2 applications.

[0219] Investigation method: The test was conducted 10 days after the last application of pesticides. Five points were selected on the diagonal of each plot, with 5 plants at each point. All leaves of each plant were investigated, and the diseased leaf rate was recorded using the 9-level grading method. The disease index and control effect were calculated.

[0220] Grading Standard:

[0221] Level 0: no lesions;

[0222] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0223] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0224] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0225] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0226] Level 9: The lesion area accounts for more than 50% of the total leaf area.

[0227] The efficacy is calculated according to the following formula:

[0228]

[0229]

[0230] Results and analysis:

[0231] Table 7 Field efficacy test results of different pesticide treatments on potato late blight

[0232]

[0233] The test results in the above table show that the test agent has significant control effect on potato late blight, and the control effect of 20% mandipropamid·fluoxapiprolin suspension (1:1) is 89.13%.

[0234] According to the results of indoor and field efficacy tests, it can be seen that the fungicidal composition of the present invention has excellent control effects on Oomycete diseases, is safe for target crops, has no pesticide damage certificate, effectively reduces the dosage of pesticides used, reduces production costs, and alleviates environmental pollution.

Claims

1. A fungicidal composition containing mandelin, characterized in that: The bactericidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is mandipropamid, the active ingredient B is flufenoxadiazam, and the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 30:

1.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:30 to 10:

1.

3. The bactericidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:20 to 5:

1.

4. The bactericidal composition according to claim 1, characterized in that The total weight of the bactericidal composition is 100%wt, and the total weight of the active ingredient A and the active ingredient B accounts for 5% to 70% of the total weight of the bactericidal composition.

5. The bactericidal composition according to claim 1, characterized in that In addition to the active ingredients, the fungicide composition also includes agriculturally permitted auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

6. The bactericidal composition according to claim 1, characterized in that The bactericidal composition can be prepared into any formulation allowed in agriculture, and the formulation is selected from a solid formulation or a liquid formulation; The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules; The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions or microcapsule suspension-suspoemulsions.

7. Use of the fungicidal composition according to any one of claims 1 to 6 for preventing and controlling plant oomycete diseases.

8. The use according to claim 7, characterized in that The Oomycete diseases are diseases caused by the following pathogenic bacteria: pathogenic bacteria of the Pythium family, pathogenic bacteria of the Peronophyte family, pathogenic bacteria of the Downy Mildew family, and pathogenic bacteria of the Albuminopsis family.

9. The use according to claim 8, characterized in that The pathogenic bacteria of the Pythium family are Pythium, Phytophthora, Phytophthora, Phytophthora or Phytophthora; the pathogenic bacteria of the Peronophthora family are Peronophthora litchii; the pathogenic bacteria of the Peronosporaceae family are Peronospora spp., Peronospora spp., Peronospora spp., Peronospora spp., Peronospora spp., Peronospora spp., Peronospora spp., Peronospora spp., Peronospora spp., Peronospora spp., Peronospora spp. or Pseudocoperonospora spp.

10. The use according to claim 7, characterized in that The bactericidal composition or its preparation is applied to the pathogens to be controlled or their growth medium.

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