A bactericidal composition and its application

Abstract

The present invention belongs to the field of pesticide sterilization technology and discloses a sterilization composition and its use. The sterilization composition comprises active ingredient A and active ingredient B, wherein active ingredient A is any one of tetrazolium pyraclostrobin, ethaboxam, or indazole sulfaquinoxaline; active ingredient B is fluoxapiprolin; and the mass ratio of active ingredient A to active ingredient B is 1:40 to 45:1. The sterilization composition of the present invention can effectively prevent and control plant oomycete diseases, while preventing and controlling diseases, and can slow the development and progression of drug resistance in pathogens. It has a significant sterilization effect and is safe for crops.

Description

[0001] This invention application is a divisional application with application number CN202310201238.X, application date March 6, 2023, and invention name “A bactericidal composition and its application”. Technical Field

[0002] The invention belongs to the technical field of pesticide sterilization, and specifically discloses a sterilization composition and application thereof. Background Art

[0003] Tetrazolylpyramid is a new type of carbamate fungicide, CAS registration number: 500207-04-5. It can effectively control diseases caused by oomycetes, such as downy mildew, Pythium, Pseudoconopsis and Phytophthora, and has excellent preventive effect on crop downy mildew and blight.

[0004] Ethiazolin, CAS registration number: 162650-77-3, chemical name: N-[cyano(thien-2-yl)methyl]-4-ethyl-2-(ethylamino)-1,3-thiazole-5-carboxamide, has a mechanism of action of inhibiting β-tubulin assembly in mitosis. It is an inhibitor of mitosis and cell division and can prevent and control diseases caused by various oomycete pathogens.

[0005] Amisulbrom, common name in English: amisulbrom, CAS registration number 348635-87-0, chemical name is 3-(3-bromo-6-fluoro-2-methylindol-1-yl)sulfonyl-N,N-dimethyl-1,2,4-triazole-1-sulfonamide, which is a triazole sulfonamide fungicide that is mainly effective against oomycetes and proteobacteria.

[0006] Fluoxapiprolin is a novel piperidinethiazole isoxazoline fungicide with the CAS registration number 1771741-86-6. Its mechanism of action is as an inhibitor of oxysterol-binding protein homologs. The R- and S-enantiomers of fluoxapiprolin possess nearly identical biological activity, demonstrating excellent efficacy against oomycete pathogens, such as downy mildew and blight, in crops like potato, grape, and tomato.

[0007] After research, the inventors found that compounding any one of tetrazolium pyraclostrobin, ethaboxam or indazole sulfaquinoxaline with fluoxapiprolin in a suitable ratio can improve the prevention and control effect of oomycete diseases, reduce agricultural production costs, help avoid the occurrence of pathogen resistance and slow the rate of drug resistance development, and solve the problems of severe resistance and high drug costs in the current prevention and control of oomycete diseases. Summary of the Invention

[0008] Based on the above situation, the purpose of the present invention is to provide a fungicide composition, which can effectively prevent and control plant oomycete diseases, and while preventing and controlling diseases, it can effectively slow down the generation and development of drug resistance in pathogens, has a significant fungicidal effect, and is safe for crops.

[0009] In order to achieve the above object, the present invention adopts the following technical solution: a fungicide composition, which comprises active ingredient A and active ingredient B, wherein the active ingredient A is any one of tetrazolium pyraclostrobin, ethaboxam or indazole sulfaquinoxaline, and the active ingredient B is fluoxapiprolin.

[0010] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 45:1;

[0011] Furthermore, the active ingredient A is tetrazolium pyrrolidone, and the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 30:1;

[0012] The active ingredient A is tetrazolium pyrrolidone, and the mass ratio of the active ingredient A to the active ingredient B is 1:40, 1:35, 1:25, 1:20, 1:10, 1:8, 1:5, 1:4, 1:2, 1:1, 3:1, 4:1, 8:1, 10:1, and 30:1;

[0013] The active ingredient A is tetrazolium pyrrolidone, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1;

[0014] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:20, 1:10, 1:8, 1:5, 1:4, 1:2, 1:1, 3:1, 4:1, 8:1, 10:1, and 30:1;

[0015] The active ingredient A is tetrazolium pyrrolidone, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 30:1;

[0016] The active ingredient A is tetrazolium pyrrolidone, and the mass ratio of the active ingredient A to the active ingredient B is 1:20, 1:10, 1:8, 1:5, 1:4, 1:2, 1:1, 3:1, 4:1, 8:1, 10:1, and 30:1;

[0017] Furthermore, the active ingredient A is ethaboxam, and the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 30:1.

[0018] The active ingredient A is ethaboxam, and the mass ratio of the active ingredient A to the active ingredient B is 1:40, 1:30, 1:10, 1:5, 1:3, 1:1, 3:1, 10:1, 20:1, and 30:1;

[0019] The active ingredient A is ethaboxam, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 20:1;

[0020] The active ingredient A is ethaboxam, and the mass ratio of the active ingredient A to the active ingredient B is 1:30, 1:10, 1:5, 1:3, 1:1, 3:1, 10:1, and 20:1;

[0021] The active ingredient A is ethaboxam, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 10:1;

[0022] The active ingredient A is ethaboxam, and the mass ratio of the active ingredient A to the active ingredient B is 1:10, 1:5, 1:3, 1:1, 3:1, and 10:1;

[0023] Furthermore, the active ingredient A is indazole sulfamethoxazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 30:1;

[0024] The active ingredient A is indazole sulfamethoxazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30, 1:25, 1:15, 1:10, 1:8, 1:5, 1:4, 1:3, 1:1, 3:1, 4:1, 5:1, 8:1, 10:1, 15:1, 20:1, and 30:1;

[0025] The active ingredient A is indazole sulfamethoxazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 20:1;

[0026] The active ingredient A is indazole sulfamethoxazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:15, 1:10, 1:8, 1:5, 1:4, 1:3, 1:1, 3:1, 4:1, 5:1, 8:1, 10:1, 15:1, and 20:1;

[0027] The active ingredient A is indazole sulfamethoxazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 10:1;

[0028] The active ingredient A is indazole sulfamethoxazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:10, 1:8, 1:5, 1:4, 1:3, 1:1, 3:1, 4:1, 5:1, 8:1, and 10:1.

[0029] Furthermore, the total weight of the bactericidal composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 1% to 80% of the bactericidal composition;

[0030] Furthermore, the fungicidal composition contains, in addition to the active ingredient, pesticide-acceptable auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer or a carrier;

[0031] Furthermore, the wetting agent is selected from a mixture of one or more of sodium lauryl sulfate, sodium dodecylbenzene sulfonate, lakai powder BX, wetting and penetrating agent F, saponin powder, silkworm excrement or soapberry powder;

[0032] The dispersant is selected from a mixture of one or more of polycarboxylates, lignin sulfonates, alkylphenol polyoxyethylene ether formaldehyde condensate sulfates, alkylbenzene sulfonate calcium, naphthalenesulfonic acid formaldehyde condensate sodium salts, alkylphenol polyoxyethylene ethers, fatty amine polyoxyethylene ethers, fatty acid polyoxyethylene ethers or glycerol fatty acid ester polyoxyethylene ethers;

[0033] The thickener is selected from a mixture of one or more of xanthan gum, disintegrant, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;

[0034] The disintegrant is selected from a mixture of one or more of bentonite, urea, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;

[0035] The antifreeze agent is selected from a mixture of one or more of ethylene glycol, propylene glycol, glycerol or urea;

[0036] The defoaming agent is selected from silicone oil, silicone compounds, C 10 ~C 20 Saturated fatty acid compounds or C8~C 10 A mixture of one or more fatty alcohol compounds;

[0037] The solvent is selected from a mixture of one or more of N,N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate or acetonitrile;

[0038] The preservative is selected from a mixture of one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one;

[0039] The stabilizer is selected from a mixture of one or more of epoxy soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0040] The carrier is selected from a mixture of one or more components selected from ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0041] Furthermore, the fungicide composition can be prepared into any formulation acceptable for pesticides, and the formulation is selected from powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsions, water-dispersible granules, emulsions, water-dispersible tablets, soluble powders, soluble tablets, soluble granules, soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible liquids, pastes, emulsions in water, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-in-water emulsions, or microcapsule suspension-suspoemulsions;

[0042] Further, the formulation is selected from a suspension concentrate, a dispersible oil suspension concentrate, a wettable powder or a water dispersible granule;

[0043] The present invention also discloses the use of the above-mentioned fungicide composition for preventing and controlling plant oomycete diseases.

[0044] Furthermore, the plant oomycete disease is potato late blight, tomato late blight, pepper blight, cucumber downy mildew or grape downy mildew;

[0045] Furthermore, the plant oomycete disease is potato late blight or cucumber downy mildew;

[0046] Furthermore, the fungicide composition is used to prevent and control cucumber downy mildew, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1;

[0047] Furthermore, the fungicide composition is used to prevent and control cucumber downy mildew, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 20:1;

[0048] Furthermore, the fungicide composition is used to prevent and control potato late blight, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 30:1;

[0049] Furthermore, the fungicide composition is used to prevent and control potato late blight, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 30:1;

[0050] Furthermore, the fungicide composition and / or its preparation is applied to the medium where the disease to be prevented and controlled occurs.

[0051] The beneficial effects of the present invention are as follows:

[0052] 1) The fungicidal composition of the present invention exhibits a significant synergistic effect within a certain ratio range, thereby improving the control effect on pathogens, reducing the dosage of pesticides, and being safe for crops;

[0053] 2) The two active ingredients in the bactericidal composition of the present invention have different mechanisms of action, which effectively slows down the generation and development of drug resistance in pathogens and prolongs the service life of the product. DETAILED DESCRIPTION

[0054] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to illustrate the present invention and are not intended to limit the present invention.

[0055] Preparation example:

[0056] Preparation Example 1: 24% tetrazolium pyrazone·fluoxapiprolin suspension (1:1)

[0057] Formula composition: 12% tetrazolium pyrrolidone, 12% fluoxapiprolin, 2% fatty alcohol polyoxyethylene ether, 1% sodium salt of polycarboxylate, 3% styrenated phenol polyoxyethylene ether sulfate, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 0.2% magnesium aluminum silicate, 1% xanthan gum, 5% propylene glycol, 0.3% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0058] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0059] Preparation Example 2: 21% tetrazolium pyrazone·fluoxapiprolin suspension (2:1)

[0060] Formula composition: 14% tetrazolium pyrrolidone, 7% fluoxapiprolin, 1% fatty alcohol polyoxyethylene ether, 4% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 2% naphthalenesulfonate formaldehyde condensate, 4% styrenated phenol polyoxyethylene ether phosphate, 1% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1.5% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0061] Preparation method: Same as Preparation Example 1.

[0062] Preparation Example 3: 25% tetrazolium pyrazone·fluoxapiprolin dispersible oil suspension (1:4)

[0063] Formula composition: 5% tetrazolium pyrazone, 20% fluoxapiprolin, 2% naphthalenesulfonate formaldehyde condensate, 2% fatty alcohol polyoxyethylene ether, 10% alkylaryl polyoxyethylene polyoxypropylene ether, 3% calcium dodecylbenzenesulfonate, 1% silicon dioxide, 2% organic bentonite, and methyl oleate makes up the balance;

[0064] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, oil is added and mixed evenly, and the dispersible oil suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0065] Preparation Example 4: 16% tetrazolium pyrazone·fluoxapiprolin dispersible oil suspension (3:1)

[0066] Formula composition: 12% tetrazolium pyrazone, 4% fluoxapiprolin, 5% alkylphenol polyoxyethylene ether, 12% castor oil polyoxyethylene ether, 1% calcium dodecylbenzenesulfonate, 1% sodium polycarboxylate, and soybean oil makes up the balance;

[0067] Preparation method: Same as Preparation Example 3.

[0068] Preparation Example 5: 36% tetrazolium pyrazone-fluoxapiprolin water dispersible granules (1:8)

[0069] Formula composition: 4% tetrazolium pyrrolidone, 32% fluoxapiprolin, 5% sodium lignin sulfonate, 10% sodium polycarboxylate, 3% sodium lauryl sulfate, 5% white carbon black, and kaolin makes up the balance;

[0070] Preparation method: According to the formula ratio of the embodiment, the active ingredient is added to the carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then kneading, granulation, drying, and sieving are carried out to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0071] Preparation Example 6: 30% tetrazolium pyrazone-fluoxapiprolin water dispersible granules (2:1)

[0072] Formula composition: 20% tetrazolium pyrrolidone, 10% fluoxapiprolin, 8% lignin sulfonate, 3% sodium dodecylbenzenesulfonate, 3% sodium lauryl sulfate, 5% white carbon black, 30% starch, and kaolin makes up the balance.

[0073] Preparation method: Same as Preparation Example 5.

[0074] Preparation Example 7: 24% ethaboxam·fluoxapiprolin suspension (1:3)

[0075] Formula composition: 6% ethaboxam, 18% fluoxapiprolin, 1% sodium lauryl sulfate, 2% naphthalenesulfonate formaldehyde condensate, 3% alkylphenol polyoxyethylene ether, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% propylene glycol, 0.01% potassium benzisothiazolinone, 0.5% silicone oil, and deionized water to make up the balance;

[0076] Preparation method: Same as Preparation Example 1.

[0077] Preparation Example 8: 24% ethaboxam·fluoxapiprolin suspension (1:5)

[0078] Formula composition: 4% ethaboxam, 20% fluoxapiprolin, 2% fatty alcohol polyoxyethylene ether, 3% alkylphenol polyoxyethylene ether, 2% styrenated phenol polyoxyethylene ether sulfate, 2% sodium polycarboxylate, 1.5% magnesium aluminum silicate, 0.2% sodium benzoate, 5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0079] Preparation method: Same as Preparation Example 1.

[0080] Preparation Example 9: 30% ethaboxam·fluoxapiprolin water dispersible granules (1:1)

[0081] Formula composition: 15% ethaboxam, 15% fluoxapiprolin, 6% sodium lignin sulfonate, 3% BX powder, 2% sodium dodecylbenzene sulfonate, 7% ammonium sulfate, and kaolin makes up the balance;

[0082] Preparation method: Same as Preparation Example 5.

[0083] Preparation Example 10: 32% ethaboxam·fluoxapiprolin water dispersible granules (1:3)

[0084] Formula composition: 8% ethaboxam, 24% fluoxapiprolin, 8% sodium lignin sulfonate, 3% sodium polycarboxylate, 2% sodium lauryl sulfate, 8% ammonium sulfate, and starch makes up the balance.

[0085] Preparation method: Same as Preparation Example 5.

[0086] Preparation Example 11: 15% indazole sulfamethoxazole·fluoxapiprolin suspension (1:1)

[0087] Formula composition: 7.5% indazole sulfamethoxazole, 7.5% fluoxapiprolin, 1% fatty alcohol polyoxyethylene ether, 2% alkylphenol polyoxyethylene ether phosphate, 1% polycarboxylic acid sodium salt, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% glycerol, 0.1% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0088] Preparation method: Same as Preparation Example 1.

[0089] Preparation Example 12: 20% indazole sulfamethoxazole·fluoxapiprolin suspension (1:3)

[0090] Formula composition: 5% indazole sulfamide, 15% fluoxapiprolin, 2% isotridecyl alcohol polyoxyethylene ether, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% polycarboxylic acid sodium salt, 3% styrenated phenol polyoxyethylene ether phosphate, 0.5% magnesium aluminum silicate, 0.2% xanthan gum, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0091] Preparation method: Same as Preparation Example 1.

[0092] Preparation Example 13: 36% indazole sulfamethoxazole and fluoxapiprolin water dispersible granules (1:8)

[0093] Formula composition: 4% indazole sulfamethoxazole, 32% fluoxapiprolin, 8% polycarboxylic acid sodium salt, 6% dispersant NNO, 3% sodium dodecylbenzene sulfonate, 2% sodium lauryl sulfate, 4% sodium sulfate, and kaolin makes up the balance;

[0094] Preparation method: Same as Preparation Example 5.

[0095] Preparation Example 14: 30% indazole sulfamethoxazole and fluoxapiprolin water dispersible granules (1:4)

[0096] Formula composition: 6% indazole sulfanilamide, 24% fluoxapiprolin, 9% lignin sulfonate, 4% sodium dodecylbenzene sulfonate, 1.5% sodium dodecylbenzene sulfonate, 5% white sugar, kaolin makes up the balance;

[0097] Preparation method: Same as Preparation Example 5.

[0098] Preparation Example 15: 30% indazole sulfamethoxazole·fluoxapiprolin wettable powder (1:5)

[0099] Formula: 5% indazole sulfamethoxazole, 25% fluoxapiprolin, 4% naphthalenesulfonate formaldehyde condensate, 2% sodium lauryl sulfate, 5% white carbon black, 8% tea saponin, and kaolin to make up the balance;

[0100] Preparation method: Active ingredients, dispersants, wetting agents and fillers are mixed according to the formula ratio, stirred evenly in a stirring kettle, and pulverized and mixed evenly for multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0101] Preparation Example 16: 18% indazole sulfamethoxazole·fluoxapiprolin wettable powder (1:1)

[0102] Formula: 9% indazole sulfamethoxazole, 9% fluoxapiprolin, 5% dispersant NNO, 8% sodium lignin sulfonate, 2% sodium lauryl sulfate, and kaolin to make up the balance;

[0103] Preparation method: Same as Preparation Example 15.

[0104] Example 1: Indoor bioactivity test of different agents against cucumber downy mildew

[0105] Test basis: The test refers to NY / T 1156.7-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 7: Pot Method for Control of Cucumber Downy Mildew".

[0106] Test target: Cucumber downy mildew (Pseudoperonospora cubensis).

[0107] Test instruments and equipment: electronic balance, spray equipment, artificial climate chamber, biological incubator, culture dish, pipette, etc.

[0108] Preparation of test materials: Select susceptible cucumber varieties (Xintai Mici) for potting and set aside when the seedlings have grown to 4 to 6 true leaves.

[0109] Test agents: indazolesulfamide technical, fluoxapiprolin technical, and tetrazolium pyrazone technical, provided by the Group's R&D Center.

[0110] Test steps:

[0111] (1) Preparation of sporangium suspension

[0112] Select the infected cucumber leaves and wash the sporangia of downy mildew on the back of the leaves with 4℃ distilled water, and prepare a suspension (concentration of 1×10 per ml). 5 ~1×10 7 Sporangium) and stored at 4℃ for later use.

[0113] (2) Preparation of pharmaceutical preparations

[0114] Dissolve the original drug in a suitable solvent and then dilute it with 0.1% Tween 80 aqueous solution. Set five series of mass concentrations according to the activity of the drug.

[0115] (3) Chemical treatment

[0116] According to the experimental design, each treatment agent was evenly sprayed on both sides of the leaves until they were completely wet. The solution was allowed to air dry before use. A treatment without agent was set up as a blank control.

[0117] (4) Inoculation and culture

[0118] Fresh sporangium suspension was sprayed on the underside of leaves, with 5 pots per treatment and 2 plants per pot, and each treatment was repeated 4 times. After inoculation, the plants were cultured under a photoperiod of 12h light:12h dark, a temperature of 17-22°C, and a relative humidity of 92%-95%.

[0119] Experimental investigation:

[0120] Based on the disease status of the blank control, the inoculated leaves were graded. Thirty leaves were surveyed for each treatment, and the following grading method was used for the graded survey and recorded:

[0121] Level 0: no disease;

[0122] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0123] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0124] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0125] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0126] Level 9: The lesion area accounts for more than 50% of the entire leaf area;

[0127] Data calculation:

[0128] Based on the data survey, the disease index and prevention and control effect of each treatment were calculated.

[0129] The disease index is calculated according to the following formula:

[0130]

[0131] The control effect is calculated according to the following formula:

[0132]

[0133] Statistical analysis:

[0134] Use DPS statistical analysis system to analyze and calculate EC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0135] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0136]

[0137] Where:

[0138] ATI - measured toxicity index of mixture;

[0139] S——EC of standard agent 50 , the unit is milligrams per liter (mg / L);

[0140] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0141] TTI=TI A ×P A +TI B ×P B

[0142] Where:

[0143] TTI – Theoretical Toxicity Index of Mixtures;

[0144] TI A ——Agent toxicity index;

[0145] P A ——The percentage of agent A in the mixture, in percentage (%);

[0146] TI B ——Toxicity index of agent B;

[0147] P B ——The percentage of agent B in the mixture, in percentage (%).

[0148]

[0149] Where:

[0150] CTC – Co-toxicity coefficient;

[0151] ATI - measured toxicity index of mixture;

[0152] TTI - Theoretical Toxicity Index of Mixture.

[0153] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0154] The indoor test results are shown in the following table:

[0155] Table 1 Results of indoor bioactivity test on cucumber downy mildew with different pesticides

[0156] Test drug Regression equation (Y = a + bx) R <![CDATA[EC 50 (mg / L)]]> Co-toxicity coefficient (CTC) Tetrazolylpyrrolidone (A) y=4.4304+1.2713x 0.9913 2.8055 - fluoxapiprolin(B) y=4.6413+1.3597x 0.9976 1.8357 - A:B (1:40) y=4.5540+1.4390x 0.9948 2.0416 90.679 A:B (1:35) y=4.6735+1.4270x 0.9960 1.6936 109.441 A:B(1:25) y=4.8484+0.9712x 0.9988 1.4325 129.873 A:B(1:10) y=4.8813+0.9631x 0.9980 1.3280 142.715 A:B (1:8) y=4.9098+1.0355x 0.9974 1.2222 156.196 A:B (1:4) y=4.9469+1.0752x 0.9919 1.1204 176.012 A:B (1:1) y=4.8931+1.2442x 0.9964 1.2188 182.087 A:B (4:1) y=4.7432+1.2962x 0.9957 1.5779 160.809 A:B (8:1) y=4.7095+1.2598x 0.9972 1.7006 155.824 A:B (10:1) y=4.6906+1.0904x 0.9948 1.9222 139.264 A:B (20:1) y=4.6123+1.2025x 0.9961 2.1010 130.255 A:B (30:1) y=4.6379+1.0229x 0.9941 2.2594 122.089

[0157] As shown in the above table (Table 1), cucumber downy mildew is more sensitive to fluoxapiprolin. A rational combination of tetrazolium pyrrolidone and fluoxapiprolin significantly enhances their effectiveness against cucumber downy mildew. When the mass ratio of tetrazolium pyrrolidone to fluoxapiprolin is between 1:40 and 30:1, the combined effect against cucumber downy mildew is additive or synergistic. The combination exhibits the most synergistic effect, with a mass ratio of 1:25 to 30:1, achieving significant control efficacy.

[0158] Table 2 Results of indoor activity test of indazolesulfamide and fluoxapiprolin against cucumber downy mildew

[0159]

[0160] The results in Table 2 show that fluoxapiprolin has a high toxicity against cucumber downy mildew, EC 50 The EC value of indazolesulfamide against cucumber downy mildew is 1.7712 mg / L. 50 The concentration of indazole sulfanil in the concentration of fluoxapiprolin was 3.1385 mg / L. When the mass ratio of indazole sulfanil to fluoxapiprolin was between 1:40 and 30:1, a synergistic effect was observed, and the synergistic effect was most obvious when the mass ratio of indazole sulfanil to fluoxapiprolin was 1:3, with a co-toxicity coefficient of 178.717.

[0161] Example 2: Indoor bioactivity test of different pesticides against Phytophthora infestans

[0162] Test basis: The test refers to NY / T 1156.2-2006 "Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Pathogenic Fungal Mycelial Growth".

[0163] Test instruments: electronic balance, puncher, inoculator, artificial climate chamber, pipette, culture dish, etc.

[0164] Test agents: fluoxapiprolin technical, tetrazolium pyraclostrobin technical, and ethaboxam technical, provided by the Group's R&D Center.

[0165] Preparation of the drug: Dissolve the above stock drug in a suitable solvent and then dilute with 0.1% Tween 80 aqueous solution. Set five series of mass concentrations according to the drug activity.

[0166] Test method: After the melted RSA culture medium is cooled to 60℃~70℃, it is quantitatively added to a sterile conical flask. From low concentration to high concentration, the test single agent and the mixed agent of the two are quantitatively drawn in sequence, and added to the above-mentioned conical flasks respectively, and shaken thoroughly. Then pour equal amounts into the culture dish to make drug-containing plates of corresponding concentrations. After the culture medium has cooled sufficiently, use a puncher to cut the bacterial cake from the edge of the colony under sterile conditions, inoculate the bacterial cake into the center of the drug-containing plate with an inoculator, place it in an incubator for culture (20℃±1℃), and investigate after 7 days. During the investigation, use a caliper to measure the diameter of the colony. Use the cross-cross method to measure the diameter of each colony vertically once, and take the average value.

[0167] Data statistics and analysis:

[0168] According to the survey results, the mycelial growth inhibition rate of each treatment on the target fungus was calculated in percentage (%), and the calculation results were rounded to two decimal places.

[0169] D=D1-D2

[0170] Where:

[0171] D——colony growth diameter;

[0172] D1 - colony diameter;

[0173] D2——diameter of mushroom cake.

[0174]

[0175] I——hyphae growth inhibition rate;

[0176] D0——blank control colony growth diameter;

[0177] D t ——The growth diameter of the colony after treatment with chemicals.

[0178] Use DPS statistical analysis system to analyze and calculate EC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0179] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0180]

[0181] Where:

[0182] ATI - measured toxicity index of mixture;

[0183] S——EC of standard agent 50 , the unit is milligrams per liter (mg / L);

[0184] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0185] TTI=TI A ×P A +TI B ×P B

[0186] Where:

[0187] TTI – Theoretical Toxicity Index of Mixtures;

[0188] TI A ——Agent toxicity index;

[0189] P A ——The percentage of agent A in the mixture, in percentage (%);

[0190] TI B ——Toxicity index of agent B;

[0191] P B ——The percentage of agent B in the mixture, in percentage (%).

[0192]

[0193] Where:

[0194] CTC – Co-toxicity coefficient;

[0195] ATI - measured toxicity index of mixture;

[0196] TTI - Theoretical Toxicity Index of Mixture.

[0197] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0198] The indoor test results are shown in the following table:

[0199] Table 3 Results of indoor bioactivity test on potato late blight under different pesticide treatments

[0200] Test drug Regression equation (Y = a + bx) R <![CDATA[EC 50 (mg / L)]]> Co-toxicity coefficient (CTC) Tetrazolylpyrrolidone (A) y=5.4578+1.1809x 0.9997 0.4095 - fluoxapiprolin(B) y=5.8239+1.1793x 0.9997 0.2001 - A:B(1:20) y=5.9440+1.2055x 0.9996 0.1648 124.450 A:B(1:10) y=5.9597+1.1834x 0.9993 0.1545 135.829 A:B (1:5) y=5.9535+1.1842x 0.9994 0.1566 139.682 A:B(1:2) y=5.8956+1.1653x 0.9989 0.1704 141.558 A:B (1:1) y=5.8820+1.1879x 0.9998 0.1809 148.610 A:B (2:1) y=5.8556+1.2037x 0.9996 0.1946 156.011 A:B (3:1) y=5.8087+1.1874x 0.9999 0.2084 155.750 A:B (10:1) y=5.6908+1.2321x 0.9991 0.2750 135.973 A:B (30:1) y=5.6238+1.1803x 0.9993 0.2962 133.737

[0201] The test results showed (Table 3) that potato late blight pathogens were highly sensitive to tetrazolium pyrazone and fluoxapiprolin. 50 The mass ratio of tetrazolium pyrazone to fluoxapiprolin is 1:20 to 30:1, and the co-toxicity coefficient is greater than 120, indicating a synergistic effect.

[0202] Table 4 Indoor activity test of different pesticide treatments against Phytophthora infestans

[0203] Test drug Regression equation (Y = a + bx) R <![CDATA[EC 50 (mg / L)]]> Co-toxicity coefficient (CTC) ethaboxam (A) y=6.5228+1.1696x 0.9995 0.0499 - fluoxapiprolin(B) y=5.8344+1.1261x 0.9971 0.1816 - A:B (1:40) y=5.9209+1.1220x 0.9975 0.1511 112.917 A:B(1:30) y=6.0006+1.1331x 0.9991 0.1309 127.847 A:B(1:10) y=6.1560+1.1798x 0.9998 0.1047 139.885 A:B (1:5) y=6.2756+1.1863x 0.9994 0.0841 149.966 A:B (1:3) y=6.2812+1.1037x 0.9993 0.0691 158.335 A:B (1:1) y=6.4529+1.1353x 0.9992 0.0525 149.120 A:B (3:1) y=6.6155+1.1700x 0.9996 0.0416 146.516 A:B (10:1) y=6.6398+1.1793x 0.9998 0.0407 131.258 A:B (20:1) y=6.6304+1.1804x 0.9986 0.0416 124.243 A:B (30:1) y=6.5396+1.2001x 0.9920 0.0521 98.072

[0204] The test results in Table 4 show that a reasonable combination of ethaboxam and fluoxapiprolin has a good control effect on potato late blight. The mass ratio of ethaboxam to fluoxapiprolin is in the range of 1:30 to 20:1, the co-toxicity coefficient is greater than 120, and the combined effect shows a synergistic effect.

[0205] Example 3: Indoor activity test of indazolesulfamide and fluoxapiprolin against grape downy mildew

[0206] Test agents: indazole sulfamethoxazole technical and fluoxapiprolin technical, provided by the group's R&D center.

[0207] Test pathogen: grape downy mildew (Plasmopara viticola).

[0208] Preparation: Dissolve the indazole sulfamethoxazole technical drug in N,N-dimethylformamide (DMF) and the fluoxapiprolin technical drug in acetone. Add 0.1% Tween 80 to each dissolved agent to prepare a stock solution. Based on the agent's activity, each technical drug was prepared into five concentration series. Pour 20 mL into sterile Petri dishes. A blank control containing only the corresponding solvent and no agent was used. Each treatment was replicated four times.

[0209] Preparation of spore suspension: Wash the sporangium on the surface of the diseased leaves with distilled water, place the diseased leaves at 25℃ and keep them moist for 24 hours to culture fresh spores. After 24 hours, use a clean brush to brush the fresh spores into sterile distilled water to prepare a spore suspension. Using a microscope and a Neubauer hemocytometer, adjust the concentration of the sporangium suspension to 5×10 5 / mL, for future use.

[0210] Inoculation: The leaf disc method was used. Leaf discs with a diameter of 1 cm were cut from the fourth or fifth leaf of a disease-free grape branch of the current year using a borer. These discs were placed with the back of the leaf facing upward on the surface of the drug solution in a Petri dish (15 leaf discs per dish). 20 μL of sporangium suspension was inoculated in the center of each disc. The discs were cultured in a biological incubator at 25°C, approximately 80% humidity, and 12 hours of light per day.

[0211] Data statistics and analysis: When the blank control leaf disc is evenly diseased, investigate the disease situation. Grade and record the diseased leaves according to the percentage of lesion area to leaf disc area.

[0212] Grape downy mildew grading standards (based on leaves) are:

[0213] Grade 0, no lesions;

[0214] Level 1: the lesion area accounts for less than 5% of the entire leaf area;

[0215] Level 3, the lesion area accounts for 6% to 25% of the entire leaf area;

[0216] Level 5: the lesion area accounts for 26% to 50% of the entire leaf area;

[0217] Level 7, the lesion area accounts for 51% to 75% of the entire leaf area;

[0218] Level 9: The lesion area accounts for more than 76% of the entire leaf area.

[0219] The disease index is calculated according to the following formula:

[0220]

[0221] The control effect is calculated according to the following formula:

[0222]

[0223] Statistical analysis:

[0224] Analyze using DPS statistical analysis system to calculate EC 50 The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0225]

[0226] Where:

[0227] ATI - measured toxicity index of mixture;

[0228] S——EC of standard agent 50 , the unit is milligrams per liter (mg / L);

[0229] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0230] TTI=TI A ×P A +TI B ×P B

[0231] Where:

[0232] TTI – Theoretical Toxicity Index of Mixtures;

[0233] TI A ——Agent toxicity index;

[0234] P A ——The percentage of agent A in the mixture, in percentage (%);

[0235] TI B ——Toxicity index of agent B;

[0236] P B ——The percentage of agent B in the mixture, in percentage (%).

[0237]

[0238] Where:

[0239] CTC – Co-toxicity coefficient;

[0240] ATI - measured toxicity index of mixture;

[0241] TTI - Theoretical Toxicity Index of Mixture.

[0242] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0243] The indoor test results are shown in the following table:

[0244] Table 5 Results of indoor activity tests of indazolesulfamide and fluoxapiprolin against grape downy mildew

[0245]

[0246] The indoor activity test results in Table 5 show that the combination of indazole sulfanil and fluoxapiprolin has a good control effect on grape downy mildew. When the mass ratio of indazole sulfanil to fluoxapiprolin is 1:25 to 20:1, the co-toxicity coefficient against grape downy mildew is greater than 120, showing a synergistic effect.

[0247] Example 4: Field efficacy test of tetrazolpyrazone combined with fluoxapiprolin against potato late blight

[0248] The test was conducted in accordance with GB / T 17980.34-2000 “Guidelines for field efficacy tests (I) Fungicides for the control of potato late blight”.

[0249] Test object: potato late blight (Phytophthora infestans).

[0250] Test crop: Potato (Netherlands No. 7).

[0251] The experiment was conducted in a potato planting base in Jiaoxi Town, Jiaozhou City, Shandong Province. The experimental land has high fertility and late blight has occurred every year.

[0252] Experimental design: The experiment was conducted with 7 treatments (Table 6), each treatment was repeated 4 times, and there were 28 plots in total, with a plot area of ​​20m 2 , randomized block arrangement.

[0253] The experiment started with the application of pesticides at the early stage of potato late blight, using a single electric spray nozzle for conventional spraying, so that the pesticide solution was evenly attached to the potato leaves. The application rate of the pesticide solution was 750L / hm2. 2 The experiment was conducted with 3 applications in total and the interval between applications was 7 days.

[0254] Investigation method: Before applying the pesticide, investigate the disease base. The control effect investigation was carried out 10 days after the last application. During the investigation, samples were taken at five diagonal points in each plot, and two plants were selected at each point. All leaves were investigated and graded based on the percentage of the diseased area on each leaf to the total leaf area. The grading method is:

[0255] Level 0: no lesions;

[0256] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0257] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0258] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0259] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0260] Level 9: The lesion area accounts for more than 50% of the entire leaf area.

[0261] The efficacy is calculated according to the following formula:

[0262]

[0263] The potatoes grew normally throughout the test and no pesticide damage was found to the potato plants.

[0264] Results and Analysis:

[0265] Table 6 Results of field efficacy test on potato late blight

[0266]

[0267] It can be seen from the field efficacy test results in the above table (Table 6) that the fungicidal composition of the present invention is safe for crops, can effectively control the spread and development of potato late blight, and has a significant preventive effect.

[0268] Example 5: Field efficacy test of ethaboxam and fluoxapiprolin combination against potato late blight

[0269] Test basis: The test refers to GB / T 17980.34-2000 "Guidelines for field efficacy tests (I) Fungicides for the control of potato late blight".

[0270] Test object: potato late blight (Phytophthora infestans).

[0271] Test crop: Potato (Netherlands 15).

[0272] The experiment was carried out at a potato planting base in Sishui County, Jining City, Shandong Province. The water and fertilizer management in the experimental site was above average, which was in line with local scientific agricultural practices.

[0273] Experimental design: The experiment set up 6 treatments, each plot area was 50m 2 , randomized block arrangement, 4 replicates.

[0274] Test time: The test was carried out at the early stage of potato late blight, once every 7 days, for 2 consecutive sprayings. A Gongnong-16 type backpack sprayer was used to spray the stems and leaves of the whole potato plant.

[0275] Investigation method: Investigate the disease base before applying the pesticide, and conduct a control effect investigation 10 days after the last application of the pesticide. During the investigation, select 5 points on the diagonal of each plot, with 5 plants at each point, and investigate all the leaves of each plant. Record the diseased leaf rate using the 9-level grading method, and calculate the disease index and control effect.

[0276] Grading standards:

[0277] Level 0: no lesions;

[0278] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0279] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0280] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0281] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0282] Level 9: The lesion area accounts for more than 50% of the entire leaf area.

[0283] The efficacy is calculated according to the following formula:

[0284]

[0285] Safety investigation: During the entire trial process, no effect of the test agent on the growth and development of potatoes was found.

[0286] Results and Analysis:

[0287] Table 7 Field test results of different pesticide treatments against potato late blight

[0288]

[0289] The results of the field efficacy test show that the fungicidal composition of the present invention has a good control effect on potato late blight. When the dosage of the active ingredient is the same, the control effect is significant. Ten days after the last application, the control effects of 24% ethaboxam-fluoxapiprolin suspension concentrate (1:5), 24% ethaboxam-fluoxapiprolin suspension concentrate (1:3), and 30% ethaboxam-fluoxapiprolin water dispersible granules (1:1) are 84.98%, 87.54%, and 85.28%, respectively.

[0290] Example 6: Field efficacy test of a combination of indazolesulfamide and fluoxapiprolin against cucumber downy mildew

[0291] The test was conducted in accordance with GB / T 17980.26-2000 "Guidelines for field efficacy tests of pesticides (I) Fungicides for the control of cucumber downy mildew".

[0292] Test Location: Located in Shijiazhuangzi Village, Hanting District, Weifang City, the test site was a greenhouse cultivation site with relatively flat terrain, sandy loam soil, and ample fertilizer and water. Cucumbers were planted in late September and grew evenly, reaching the fruiting stage at the time of the test.

[0293] Test target: Cucumber downy mildew (Pseudoperonospora cubensis).

[0294] Experimental crops: cucumber (Xintai Mici).

[0295] Experimental method: This experiment set up 6 treatments, arranged in random blocks, each treatment was repeated 4 times, and each plot area was 20m 2 , isolation rows were set up between the plots. The experiment was sprayed twice in total, on October 12, 2020 and October 17, 2020. A Gongnong-16 backpack sprayer was used to spray the cucumber plants at a rate of 675L / hm 2 .

[0296] Experimental investigation: The disease baseline was assessed before application, and control efficacy was assessed 7 days after the first application and 10 days after the second application. Five sampling points were used in each plot, with two plants surveyed at each point, for a total of 10 plants. All leaves of each plant were surveyed. Disease index and control efficacy were calculated based on the percentage of diseased leaf area to total leaf area. The number of diseased leaves per treatment was recorded, and disease index and control efficacy were calculated.

[0297] During the entire test process, the safety of each agent treatment on cucumbers was observed at irregular intervals, and no adverse effects of the test agents on cucumbers were found.

[0298] Grading method:

[0299] Grade 0, no lesions;

[0300] Level 1: the lesion area accounts for less than 5% of the entire leaf area;

[0301] Level 3: the lesion area accounts for 6% to 10% of the entire leaf area;

[0302] Level 5: the lesion area accounts for 11% to 25% of the entire leaf area;

[0303] Level 7, the lesion area accounts for 26% to 50% of the entire leaf area;

[0304] Level 9: The lesion area accounts for more than 51% of the entire leaf area.

[0305] The disease index and prevention efficacy are calculated according to the following formula:

[0306]

[0307] The results of the field efficacy test are shown in the table below:

[0308] Table 8 Field efficacy test results of compound preparations against cucumber downy mildew

[0309]

[0310] Field efficacy tests showed that (Table 8), 36% indazole sulfanil·fluoxapiprolin water dispersible granules (1:8), 18% indazole sulfanil·

[0311] Ten days after the second application, a combination of fluoxapiprolin wettable powder (1:1) and 20% indazolesulfamide / fluoxapiprolin suspension concentrate (1:3) achieved 86.11% to 90.70% control efficacy. Therefore, a rational combination of indazolesulfamide and fluoxapiprolin is highly effective against cucumber downy mildew and can effectively slow the spread of the disease.

[0312] Example 7: Field efficacy test of a combination of indazolesulfamide and fluoxapiprolin against potato late blight

[0313] Test basis: The test refers to GB / T 17980.31-2000 "Guidelines for field efficacy tests of pesticides (I) Fungicides for the control of early blight and late blight of tomatoes".

[0314] Test object: potato late blight (Phytophthora infestans).

[0315] Test crop: Potato (Netherlands No. 15).

[0316] Experimental location: The experiment was set up in a potato field in Wulan Town, Jingyuan County, Gansu Province. The previous crop was potato. The soil fertility of the experimental site was medium. The potatoes were planted on April 7, 2020. They were cultivated and managed normally and grew uniformly. The cultivation fields of all experimental plots were uniform and consistent with local scientific agricultural practices (GAP).

[0317] The test drugs and dosages are shown in the table below.

[0318] Experimental plot arrangement: The experimental drug, control drug and blank control plots are randomly arranged. Each plot is 40m 2 , each treatment was repeated 4 times.

[0319] Application time: The first application was carried out at the early stage of potato late blight (July 3, 2020), and the application was carried out three times in succession, with an interval of 7 days between each application. The potatoes were in the tuber swelling stage when the application was carried out. The potato plants were sprayed with a 3WBS-16A backpack manual sprayer. The weather was clear on the day of the application, and the application rate was 900L / hm 2 .

[0320] Survey method: The disease index was investigated before application and 7 days after the last application, for a total of 2 surveys. Five random sampling points were selected in each plot, with 2 plants selected at each point. All leaves were surveyed and graded based on the percentage of the lesion area on each leaf to the total leaf area. The grades were recorded according to the following grading method:

[0321] Level 0: no lesions;

[0322] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0323] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0324] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0325] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0326] Level 9: The lesion area accounts for more than 50% of the entire leaf area;

[0327] Calculation method of experimental drug efficacy:

[0328]

[0329]

[0330] During the experiment, the potato plants were observed irregularly and no adverse effects were found in any of the treatment groups.

[0331] The results of the field efficacy test are shown in the table below:

[0332] Table 9 Field efficacy test results of compound preparations against potato late blight

[0333]

[0334] The field efficacy test on potato late blight showed (Table 9) that 7 days after the last application, the overall prevention efficacy of the fungicide composition of the present invention on potato late blight was greater than 85%, showing good long-lasting efficacy.

[0335] Example 8: Field efficacy test of a combination of indazolesulfamide and fluoxapiprolin against grape downy mildew

[0336] The test was carried out in accordance with GB / T 17980.122-2004 “Guidelines for Field Efficacy Tests of Pesticides (II) Part 122: Fungicides for Control of Grape Downy Mildew”.

[0337] Test location: The test site was set up in the vineyard of Ximazhuang Village, Liaocheng City, Shandong Province. The terrain is flat and the soil fertility is uniform. The grapes grow well. The water, fertilizer and cultivation management conditions of the test area and the control area are consistent.

[0338] Test target: Grape downy mildew (Plasmopara viticola).

[0339] The experimental crop is grape (Vitis vinifera), with a plant spacing of 0.8m×2m, 8 years old, and is cultivated in an open-air trellis style.

[0340] Experimental plot setting: The experimental agent, control agent and blank control plots were arranged in random blocks, 8 grapevines were planted in each plot, and each treatment was repeated 4 times.

[0341] Application method and investigation method: On July 3 and July 13, 2020, the stems and leaves of the grape plants were evenly sprayed with a 3WBS-16A manual sprayer. When applying the pesticide, the test agent was sprayed first, and then the control agent was sprayed in sequence from low concentration to high concentration. The sprayer was cleaned when changing the agent. The spray liquid volume was 1500L / hm 2 No other pesticides were used to control pests and diseases during the 10 days before and during the experiment.

[0342] The disease survey was conducted 10 days after the last application of the pesticide. During the survey, 10 new vines grown that year were randomly selected from each plot. The total number of leaves and diseased leaves on each branch were investigated, and the number of diseased leaves at each level and the total number of leaves were recorded according to the following grading method.

[0343] Leaf grading method:

[0344] Grade 0, no lesions;

[0345] Level 1: the lesion area accounts for less than 5% of the entire leaf area;

[0346] Level 3, the lesion area accounts for 6% to 25% of the entire leaf area;

[0347] Level 5: the lesion area accounts for 26% to 50% of the entire leaf area;

[0348] Level 7, the lesion area accounts for 51% to 75% of the entire leaf area;

[0349] Level 9: The lesion area accounts for more than 76% of the entire leaf area.

[0350] The disease index and prevention efficacy are calculated according to the following formula:

[0351]

[0352] The results of the field efficacy test are shown in the table below:

[0353] Table 10 Field efficacy test results of compound preparations against grape downy mildew

[0354]

[0355] As shown in the test results in Table 10, different mixtures of fluoxapiprolin and indazole were superior to the control agent in controlling grape downy mildew. Ten days after the last application, the fungicidal composition of the present invention achieved a 92.50% control efficacy. The control efficacy at high concentrations was superior to that at low concentrations. The control agents, a 10% fluoxapiprolin dispersible oil suspension concentrate and a 17.7% indazole suspension concentrate, achieved efficacy of 82.41% and 80.27%, respectively.

[0356] In summary, through indoor toxicity testing and field efficacy tests, it can be seen that the fungicidal composition of the present invention has a good preventive and control effect on plant oomycete diseases and is safe for crops, especially for cucumber downy mildew, grape downy mildew and potato late blight. It is superior to a single agent in delaying the generation and development of drug resistance and prolonging the lasting effect, and can effectively reduce costs and reduce environmental pressure.

[0357] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A bactericidal composition, characterized in that The bactericidal composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is ethaboxam, the active ingredient B is fluoxapiprolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 20:

1.

2. The bactericidal composition according to claim 1, characterized in that The active ingredient A is ethaboxam, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 10:

1.

3. The bactericidal composition according to claim 1, characterized in that The total weight of the bactericidal composition is 100 wt %, and the total weight of the active ingredient A and the active ingredient B accounts for 1% to 80% of the bactericidal composition.

4. The bactericidal composition according to claim 1, characterized in that In addition to the active ingredient, the fungicide composition also contains pesticide-acceptable auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

5. The bactericidal composition according to claim 1, characterized in that The bactericidal composition can be prepared into any acceptable formulation, and the formulation is selected from powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsions, water-dispersible granules, emulsions, water-dispersible tablets, soluble powders, soluble tablets, soluble granules, soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions or microcapsule suspension-suspoemulsions.

6. The bactericidal composition according to claim 5, characterized in that The formulation is selected from a suspension concentrate, a dispersible oil suspension concentrate, a wettable powder or a water-dispersible granule.

7. Use of the bactericidal composition according to any one of claims 1 to 6 for preventing and controlling plant oomycete diseases, characterized in that: The disease is potato late blight.

8. The use according to claim 7, characterized in that The fungicide composition and / or its preparation is applied to the medium where the disease to be prevented and controlled occurs.

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