Traditional Chinese medicine preparations for preventing and treating bone marrow suppression after chemotherapy

Abstract

The present invention provides a kind of Chinese medicine preparation for preventing and treating myelosuppression after chemotherapy, relates to the field of Chinese medicine pharmacy, the Chinese medicine preparation is made of 1-15 parts of antler glue, 1-10 parts of astragalus, 1-10 parts of cinnamon twig, 1-10 parts of notoginseng, 1-10 parts of immature bitter orange, 1-10 parts of rhizoma anemarrhenae, 1-10 parts of phellodendron, and the Chinese medicine preparation of the present invention has the effects such as replenishing qi and blood, promoting blood circulation and removing blood stasis, reducing swelling and relieving pain. The Chinese medicine preparation of the present invention can reverse the myelosuppression caused by 5-FU chemotherapy by improving the peripheral blood count and bone marrow pathological damage of chemotherapy mice. In addition, the Chinese medicine preparation of the present invention can improve the 5-FU chemotherapy effect by increasing the 5-FU tumor inhibition rate, suppressing tumor weight, tumor volume and promoting tumor tissue necrosis. The Chinese medicine preparation of the present invention and the chemotherapy drug 5-FU are used in combination to have a certain synergistic and toxicity-reducing effect, and are expected to be developed as a medicine for preventing and treating myelosuppression after chemotherapy.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine pharmaceutical preparations, and in particular to a traditional Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy. Background Art

[0002] Chemotherapy is the main treatment for patients with advanced tumors who are not suitable for surgery or other treatment options. It has important clinical value in the comprehensive treatment of advanced tumors. Bone marrow suppression caused by chemotherapy drugs not only affects the entire chemotherapy process, but also weakens the patient's immune function, further inducing serious complications such as infection and sepsis, making it impossible for patients to continue treatment, and seriously affecting the patient's prognosis and quality of life. In recent years, the clinical practice of using TCM dialectical thinking to apply traditional Chinese medicine therapy to the treatment of clinical chemotherapy resistance and adverse reactions has achieved relatively satisfactory results. Based on the multi-molecule and multi-target effects of traditional Chinese medicine, it is of great practical significance to develop traditional Chinese medicine and its preparations that can reduce the adverse reactions of chemotherapy and improve the effect of chemotherapy, and to combine them with chemotherapy drugs.

[0003] The Suwen (Suwen) chapter, "Yin Yang Ying Xiang Da Lun," proposes the theory that "Yang transforms into Qi, and Yin forms form." Tumor formation is the accumulation of tangible matter, or "Yin forming form." Its essence is the failure of Yin Qi to transform, resulting from dysfunctional "Yang transforming into Qi" (Yin Qi). The Lingshu (Lingshu) chapter, "The Origin of All Diseases," states, "When accumulation begins, it is exposed to cold." Physicians believe that Yang deficiency, cold stagnation, and the failure of Yin Qi to transform are the root causes of tumor formation, often playing a dominant role in their development, progression, and metastasis. Traditional Chinese Medicine (TCM) believes that Yang Qi is the fundamental force driving the flow of Qi throughout the body, involved in metabolism and cell proliferation and differentiation. Kidney Yang, the root of Yang Qi, promotes and warms the functions of various organs and tissues, including those involved in the proliferation and differentiation of immune and hematopoietic cells. Chemotherapy drugs primarily kill cancer cells by interfering with their growth and division, such as by blocking DNA replication and cell division. Traditional Chinese medicine believes that chemotherapy drugs are harsh and can harm the body's yang energy. On the one hand, they cause bone marrow suppression, leading to inhibition of immune and hematopoietic cell differentiation and proliferation, which in turn leads to low immunity and anemia. On the other hand, they cause poor circulation of the body's qi, blood, body fluids, and internal organs and meridians, aggravating the formation of a damp-heat and blood stasis environment and promoting the development of chemotherapy resistance. Therefore, clinical treatment starts with qi and blood, strengthening the spleen and stomach, replenishing the kidneys and replenishing essence, and replenishing qi and blood. The purpose of strengthening the body and replenishing the body's yang energy is to promote blood circulation and remove blood stasis to improve bone marrow hematopoietic function. The present invention aims to provide a Chinese medicine preparation that can effectively and safely treat patients with side effects such as bone marrow suppression caused by chemotherapy in clinical practice. Summary of the Invention

[0004] (1) Technical problems solved

[0005] In view of the deficiencies in the prior art, the present invention provides a traditional Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy.

[0006] (2) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] The invention provides a traditional Chinese medicine preparation for preventing and treating post-chemotherapy bone marrow suppression. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 1-15 parts of antler glue, 1-10 parts of astragalus, 1-10 parts of cinnamon twig, 1-10 parts of Panax notoginseng, 1-10 parts of immature bitter orange, 1-10 parts of anemarrhena, and 1-10 parts of phellodendron.

[0009] Furthermore, the Chinese medicine preparation is made from the following raw materials in parts by weight: 1-10 parts of antler glue, 1-5 parts of astragalus, 1-5 parts of cinnamon twig, 1-5 parts of Panax notoginseng, 1-5 parts of Citrus aurantium, 1-5 parts of Anemarrhena asphodeloides, and 1-5 parts of Phellodendron chinense.

[0010] Furthermore, the Chinese medicine preparation is made from the following raw materials in parts by weight: 6 parts of antler glue, 3 parts of astragalus, 3 parts of cinnamon twig, 3 parts of Panax notoginseng, 3 parts of Citrus aurantium, 3 parts of Anemarrhena asphodeloides, and 3 parts of Phellodendron chinense.

[0011] The traditional Chinese medicine preparation provided by the present invention is used to prepare a drug for preventing and treating bone marrow suppression after chemotherapy. The traditional Chinese medicine preparation is prepared according to conventional pharmaceutical preparation methods in pharmacy with medically acceptable excipients to be made into granules, powders, oral liquids, pills, decoctions, lyophilized powders or other pharmaceutically acceptable oral dosage forms.

[0012] (3) Beneficial effects

[0013] The present invention is based on the principle of TCM syndrome differentiation and treatment, and rationally combines various medicines to obtain a traditional Chinese medicine preparation for preventing and treating post-chemotherapy bone marrow suppression, which is prepared from antler glue, astragalus, cinnamon twig, Panax notoginseng, immature bitter orange, rhizoma anemarrhenae, and phellodendron. In the prescription, antler glue warms and nourishes the liver and kidneys, invigorates essence and nourishes blood; "Qi is the commander of blood, and blood is the mother of qi", and astragalus is an important qi-tonifying medicine, which complements antler glue and harmonizes qi and blood, and exerts a warming and nourishing effect together with antler glue and astragalus; Panax notoginseng promotes blood circulation and dissipates blood stasis, reduces swelling and relieves pain, and helps improve local blood circulation and relieve pain; bitter orange eliminates qi stagnation, resolves phlegm and disperses nodules, helps regulate qi and improves the microenvironment in the body; rhizoma anemarrhenae clears heat and nourishes yin, helps balance yin and yang in the body, and prevents warming and nourishing medicines from overheating and damaging yin; the heat-clearing and damp-drying effect of phellodendron complements that of rhizoma anemarrhenae, and together regulates the damp-heat state in the body. At the same time, phellodendron also helps eliminate bone steaming, and has a certain effect on improving symptoms caused by yin deficiency and hyperactivity of fire. This prescription uses antler glue and astragalus as the main herbs, working together to nourish blood and improve essence, tonify qi and elevate yang. Cinnamon twig, as the assistant herb, warms and unblocks the meridians, supporting yang and transforming qi. Panax notoginseng and Citrus aurantium serve as adjuvants, respectively promoting blood circulation and removing stasis, reducing swelling and relieving pain, and promoting qi circulation and dispersing stagnation, resolving phlegm and dispersing nodules. Anemarrhena astragalus and Phellodendron amurense serve as guiding herbs, clearing heat and nourishing yin, regulating dampness and heat in the body, and preventing the overheating and yin damage of warming and tonic herbs. Together, these herbs tonify qi and blood, promote blood circulation and remove stasis, reduce swelling and relieve pain, and are beneficial for preventing and treating post-chemotherapy bone marrow suppression.

[0014] The Chinese medicinal preparation of the present invention can reverse 5-FU chemotherapy-induced bone marrow suppression by improving peripheral blood counts and bone marrow pathological damage in mice undergoing chemotherapy. Furthermore, the Chinese medicinal preparation of the present invention can enhance the efficacy of 5-FU chemotherapy by increasing the 5-FU tumor inhibition rate, suppressing tumor weight and volume, and promoting tumor tissue necrosis. The Chinese medicinal preparation of the present invention, when used in combination with the chemotherapy drug 5-FU, exhibits a certain synergistic and toxicity-reducing effect, and is expected to be developed as a drug for preventing and treating post-chemotherapy bone marrow suppression. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The tumor volume, tumor weight and tumor inhibition rate of mice in each group (A); Note: K: blank group, M: model group, Y: 5-FU group, G: high-dose group of the Chinese medicine preparation of the present invention, Z: medium-dose group of the Chinese medicine preparation of the present invention, D: low-dose group of the Chinese medicine preparation of the present invention; Compared with the model group, ## P < 0.01, # P<0.05; compared with the 5-FU group, **P<0.01, *P<0.05, the same below; HE staining results of tumor tissues of mice in each group (B).

[0016] Figure 2 Figure 3 shows the number of blood cells and bone marrow nucleated cells in each group of mice (A), bone marrow pathological Gissam staining results (B), and bone marrow HE staining results (C).

[0017] Figure 3 are the levels of TGF-β1, G-CSF, TPO and EPO in the serum of mice in each group. DETAILED DESCRIPTION

[0018] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0019] Example 1

[0020] A traditional Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy is prepared from the following raw materials in parts by weight: 4 parts of antler glue, 2 parts of astragalus, 1 part of cinnamon twig, 2 parts of Panax notoginseng, 1 part of Citrus aurantium, 1 part of Anemarrhena asphodeloides, and 1 part of Phellodendron chinense.

[0021] The Chinese medicine preparation is prepared into granules, powders, oral liquids, pills, decoctions, freeze-dried powders or other pharmaceutically acceptable oral dosage forms according to conventional pharmaceutical preparation methods in pharmacy, with medically acceptable excipients.

[0022] Example 2

[0023] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is made of the following raw materials in parts by weight: 14 parts of antler glue, 8 parts of astragalus, 7 parts of cinnamon twig, 8 parts of Panax notoginseng, 6 parts of Citrus aurantium, 7 parts of Anemarrhena asphodeloides, and 7 parts of Phellodendron chinense.

[0024] Example 3

[0025] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is made of the following raw materials in parts by weight: 6 parts of antler glue, 4 parts of astragalus, 2 parts of cinnamon twig, 2 parts of Panax notoginseng, 3 parts of Citrus aurantium, 4 parts of Anemarrhena asphodeloides, and 2 parts of Phellodendron chinense.

[0026] Example 4

[0027] The difference between this embodiment and embodiment 1 is that the traditional Chinese medicine preparation is made of the following raw materials in parts by weight: 12 parts of antler glue, 6 parts of astragalus, 4 parts of cinnamon twig, 6 parts of Panax notoginseng, 8 parts of Citrus aurantium, 6 parts of Anemarrhena asphodeloides, and 7 parts of Phellodendron chinense.

[0028] Example 5

[0029] The difference between this embodiment and embodiment 1 is that the traditional Chinese medicine preparation is made of the following raw materials in parts by weight: 5 parts of antler glue, 2 parts of astragalus, 3 parts of cinnamon twig, 3 parts of Panax notoginseng, 1 part of Citrus aurantium, 2 parts of Anemarrhena asphodeloides, and 2 parts of Phellodendron chinense.

[0030] Example 6

[0031] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is made of the following raw materials in parts by weight: 8 parts of antler glue, 3 parts of astragalus, 4 parts of cinnamon twig, 2 parts of Panax notoginseng, 2 parts of Citrus aurantium, 2 parts of Anemarrhena asphodeloides, and 3 parts of Phellodendron chinense.

[0032] Example 7

[0033] The difference between this embodiment and embodiment 1 is that the traditional Chinese medicine preparation is made of the following raw materials in parts by weight: 10 parts of antler glue, 4 parts of astragalus, 3 parts of cinnamon twig, 4 parts of Panax notoginseng, 3 parts of Citrus aurantium, 2 parts of Anemarrhena asphodeloides, and 2 parts of Phellodendron chinense.

[0034] Example 8

[0035] The difference between this embodiment and embodiment 1 is that the traditional Chinese medicine preparation is made of the following raw materials in parts by weight: 6 parts of antler glue, 3 parts of astragalus, 3 parts of cinnamon twig, 3 parts of Panax notoginseng, 3 parts of Citrus aurantium, 3 parts of Anemarrhena asphodeloides, and 3 parts of Phellodendron chinense.

[0036] Test example

[0037] The Chinese medicine preparation of the present invention improves the bone marrow suppression induced by chemotherapy in mice with H22 subcutaneous tumor model of liver cancer

[0038] 1. Materials

[0039] 1.1 Experimental animals

[0040] KM female mice, body weight 18-22 g, rearing conditions: indoor temperature 22-26 ° C, relative humidity 50%-70%, circadian rhythm 12 h.

[0041] 1.2 Trial Drugs

[0042] The Chinese medicine preparation of the present invention includes: 6 parts of antler glue, 3 parts of astragalus, 3 parts of cinnamon twig, 3 parts of Panax notoginseng, 3 parts of immature bitter orange, 3 parts of anemarrhena, and 3 parts of phellodendron. The raw materials are weighed according to the above parts by weight, washed, decocted with 3 times the amount of water for 40 minutes, and the decoction is repeated for more than 2 times. The decoction is collected and concentrated to 1g / mL (1mL contains 1g of crude drug), and stored at 4°C for standby use.

[0043] 5-Fluorouracil (0.2 g, purchased from TargetMol).

[0044] 2 Methods

[0045] 2.1 Culture of H22 Hepatocellular Carcinoma Cells

[0046] The frozen H22 liver cancer cells were placed in a 37°C water bath and thawed quickly within 1 min. After thawing, they were pipetted and transferred to a 2 mL centrifuge tube. The tube was centrifuged, the supernatant was discarded, and 1.5 mL of normal saline was added to dilute the H22 cells to 1 × 10 7 / mL cell suspension, draw the cell suspension with a 2.5mL sterile syringe, and inject 0.5mL into mice intraperitoneally for subculture. After 7 days of feeding, the abdominal cavity of the mice will be enlarged, and ascites can be collected. The mice are sacrificed at the cervical vertebrae, and ascites are drawn. The resuspended tumor cells are diluted with saline to adjust the cell concentration. Aseptic technique is required throughout the entire process.

[0047] 2.2 Model establishment

[0048] Sixty KM mice were randomly divided into 10 mice as blank group, and the remaining 50 mice were subcutaneously inoculated with H22 mouse liver cancer cell suspension in the right forelimb armpit, 0.2 mL / mouse, to establish H22 mouse solid tumor model. The blank group was injected with the same volume of normal saline.

[0049] 2.3 Grouping and Dosing

[0050] The tumor formation was observed 5 days after inoculation. When the tumor grew to 100 mm 3At the same time, the mice were randomly divided into 6 groups according to the random number table method: model group, 5-FU group, high-dose, medium-dose, and low-dose groups of the Chinese herbal preparation of the present invention, with 10 mice in each group. Mice without modeling served as the blank control group and were given an equal volume of pure water orally once daily. The 5-FU group and the high-dose, medium-dose, and low-dose groups of the Chinese herbal preparation of the present invention were all given 30 mg / (kg·d) of 5-FU by intraperitoneal injection. At the same time, the high-dose, medium-dose, and low-dose groups of the Chinese herbal preparation of the present invention were given 12 g / kg, 6 g / kg, and 3 g / kg (calculated as crude drug) of the Chinese herbal preparation of the present invention, respectively, by intraperitoneal gavage once daily for 14 consecutive days. The model group was given an equal volume of normal saline orally injected every other day for 14 consecutive days.

[0051] 2.4 Index detection

[0052] 2.4.1 Tumor volume, tumor weight, and tumor inhibition rate in mice

[0053] The tumor mass was quickly removed under sterile conditions, and the morphology of the mouse tumor mass was observed with the naked eye and photographed. The tumor mass was weighed using an analytical balance, and the tumor inhibition rate was calculated. The vertical diameter and longest diameter of the tumor were measured with a vernier caliper, and the tumor volume was calculated according to the following formula:

[0054] Tumor volume = 0.5236 × ab 2 (In the formula, a is the longest diameter of the tumor and b is the vertical diameter of the tumor).

[0055] 2.4.2 Detection of mouse peripheral blood leukocyte, erythrocyte, and platelet counts

[0056] The blood of mice was collected by eyeball bleeding method. About 0.5 mL of blood was collected from each mouse and placed in an anticoagulant centrifuge tube. Diluent was added. 20 μL of the mouse blood in the anticoagulant centrifuge tube was drawn with a pipette and added to a 2 mL centrifuge tube. The number of peripheral white blood cells, red blood cells, and platelets of the mice was detected using a blood cell analyzer.

[0057] 2.4.3 Detection of G-CSF, TPO, EPO, and TGF-β1 Content in Mouse Serum

[0058] Mouse blood was collected by eyeball bleeding method, centrifuged at 3000r / min for 10min, and the serum was separated and stored at -20℃ for later use. The levels of G-CSF, TPO, EPO, and TGF-β1 in mouse serum were detected by enzyme-linked immunosorbent assay (ELISA).

[0059] 2.4.4 Detection of mouse bone marrow nucleated cells by Wright-Giemsa staining

[0060] The left femur was separated under sterile conditions, and both ends of the femur were cut off. The bone marrow cavity was flushed with physiological saline using a No. 6 needle. The flushed bone marrow cells were made into a single bone marrow cell suspension, and 5 μL was aspirated to prepare a cell smear. After natural drying, 2 to 3 drops of Wright-Giemsa complex stain were added and stained for 1 to 2 minutes. PBS buffer was added and the smear was gently shaken to mix it evenly with the Wright-Giemsa stain and then stained for another 3 to 5 minutes. The smear was washed with water and the cells were counted under a high-power microscope.

[0061] 2.4.5 Observation of tumor mass and bone marrow pathological changes in mice by HE staining

[0062] After blood collection, the mice were anesthetized with sodium pentobarbital and killed, the axillary tumors were removed, the right femurs of the mice were peeled off (decalcified with decalcification solution), fixed with 10% formaldehyde solution, dehydrated, embedded, transparent, sliced, dewaxed, and stained with hematoxylin-eosin. The pathological sections were observed using a digital pathological section scanner, and the tumor tissue and bone marrow pathology of the chemotherapy mice were graded and evaluated according to the literature.

[0063] Table 1 Evaluation of bone marrow histopathological grade in chemotherapy-treated mice (n=10)

[0064] level Degree of injury - The bone marrow tissue structure is complete, with abundant hematopoietic cells, full of nucleated cells and evenly distributed + A small number of vacuolated areas, slightly more fat cells, and less nucleated cells ++ Increased vacuolar areas, more fat cells, fewer nucleated cells, and a sparse distribution +++ The vacuolar area increased significantly, the fat cells increased significantly, the nucleated cells decreased significantly, and the cells were disordered. ++++ There are a large number of vacuolar areas, a significant increase in fat cells, and sparse and disordered distribution of nucleated cells with a significant decrease in density.

[0065] Table 2 Evaluation of tumor histopathological grade in chemotherapy-treated mice (n=10)

[0066]

[0067] 3 Statistical processing

[0068] SPSS 17.0 was used for data processing. The statistical results were presented as mean ± standard deviation. One-way ANOVA was used for comparison between groups. The LSD method was used for homogeneous variance test, and the Games-Howell method was used for unequal variance test. The Ridit test was used for ranked data.

[0069] 4. Results

[0070] 4.1 The Chinese medicine preparation of the present invention can improve the tumor volume, tumor weight and pathological changes of mice and increase the tumor inhibition rate

[0071] Compared with the model group, the tumor weight and tumor volume in the 5-FU group were significantly reduced (P < 0.01). Compared with the 5-FU group, the high and medium doses of the Chinese medicine preparation of the present invention could significantly reduce the tumor weight (P < 0.01) and tumor volume (P < 0.01, P < 0.05). The low dose of the Chinese medicine preparation of the present invention could significantly reduce the tumor weight (P < 0.05) and tumor volume (P < 0.05). All dose groups of the Chinese medicine preparation of the present invention could significantly increase the tumor inhibition rate. Figure 1 -A.

[0072] HE staining results showed that the tumor tissue cells in the model group were tightly arranged and evenly distributed, with darkly stained nuclei and a high nuclear-cytoplasmic ratio. Nuclear division was visible, and a large number of granulocytes were infiltrated in the tumor interstitium. No obvious cell necrosis was observed. Tumor tissues in the various dosage groups of the Chinese medicine preparation of the present invention showed different degrees of loose arrangement of tumor cells, vacuolation, and uneven distribution. Focal necrosis of tumor cells was observed in many places, and the nuclei of necrotic cells were condensed and fragmented. The number of mice with tumor tissue necrosis in the various dosage groups of the Chinese medicine preparation of the present invention increased significantly. The results are shown in Tables 3 and 4. Figure 1 -B.

[0073] Table 3 Results of pathological evaluation of tumor tissues in chemotherapy-treated mice (n=10)

[0074]

[0075]

[0076] Note: Compared with 5-FU group, *P<0.05, **P<0.01, compared with model group, # P<0.05, ## P<0.01

[0077] 4.2 The Chinese medicine preparation of the present invention can increase the number of blood cells and bone marrow nucleated cells in mice and improve bone marrow pathological changes

[0078] The results of peripheral blood cell detection showed that compared with the model group, the 5-FU group could significantly reduce the number of PLT, RBC, and WBC in the peripheral blood of mice (P < 0.01); compared with the 5-FU group, the high and medium doses of the Chinese medicine preparation of the present invention could significantly increase the number of PLT, RBC, and WBC (P < 0.01), and the low dose group of the Chinese medicine preparation of the present invention could also significantly increase the number of RBC, PLT, and WBC (P < 0.05). Figure 2 -A.

[0079] Giess staining results showed that compared with the model group, the 5-FU group could significantly reduce the number of nucleated cells in the bone marrow of mice (P < 0.01); compared with the 5-FU group, each dose group of the Chinese medicine preparation of the present invention could significantly increase the number of nucleated cells in the bone marrow of chemotherapy mice (P < 0.01). Figure 2 -B.

[0080] The results of bone marrow HE staining showed that the bone marrow tissue structure of the mice in the blank group and the model group was intact, with abundant hematopoietic cells and evenly distributed nucleated cells; the bone marrow tissue structure of the mice in the 5-FU group was abnormal, the number of hematopoietic cells in the bone marrow cavity was significantly reduced, a large number of vacuolar areas were visible, the number of fat cells increased, and the distribution of nucleated cells was sparse, disordered, and the density decreased; compared with the mice in the 5-FU group, the number of nucleated cells in the bone marrow of the mice in each dose group of the Chinese medicine preparation of the present invention was significantly increased, the number of fat cells was reduced, the bone marrow tissue damage was mild, and the number of mice with improved bone marrow damage was significantly increased. The results are shown in Table 4 and Figure 2 -C.

[0081] Table 4 Results of pathological evaluation of bone marrow tissue in chemotherapy-treated mice (n=10)

[0082]

[0083] Note: Compared with 5-FU group, *P<0.05, **P<0.01, compared with model group, # P<0.05, ## P<0.01

[0084] 4.3 The Chinese medicine preparation of the present invention can improve the levels of TGF-β1, G-CSF, TPO and EPO in mouse serum

[0085] ELISA test results showed that compared with the model group, the 5-FU group could significantly increase the TGF-β1 content in the serum of chemotherapy mice (P < 0.05), and significantly reduce the G-CSF, TPO, and EPO contents in the serum (P < 0.01); compared with the 5-FU group, the high- and medium-dose groups of the Chinese medicine preparation of the present invention could significantly reduce the TGF-β1 content in the serum of mice (P < 0.01), and increase the G-CSF, TPO, and EPO contents in the serum (P < 0.01); the low-dose group of the Chinese medicine preparation of the present invention could significantly reduce the TGF-β1 content in the serum of mice, and increase the G-CSF, TPO, and EPO contents in the serum (P < 0.05, P < 0.01); the specific results are shown in Figure 3 .

[0086] 5 Conclusion

[0087] The above experimental results demonstrate that the Chinese herbal preparation of the present invention can reverse 5-FU chemotherapy-induced bone marrow suppression by improving peripheral blood counts and bone marrow pathological damage in mice undergoing chemotherapy. Furthermore, the Chinese herbal preparation of the present invention can enhance the 5-FU chemotherapy efficacy by increasing the 5-FU tumor inhibition rate, suppressing tumor weight and volume, and promoting tumor tissue necrosis. The combined use of the Chinese herbal preparation of the present invention with the chemotherapy drug 5-FU has a certain synergistic and toxicity-reducing effect, and has potential development and utilization value.

[0088] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy, characterized in that: The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 1-15 parts of antler glue, 1-10 parts of astragalus, 1-10 parts of cinnamon twig, 1-10 parts of notoginseng, 1-10 parts of immature bitter orange, 1-10 parts of anemarrhena, and 1-10 parts of phellodendron.

2. A Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy according to claim 1, characterized in that: The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 1-10 parts of antler glue, 1-5 parts of astragalus, 1-5 parts of cinnamon twig, 1-5 parts of Panax notoginseng, 1-5 parts of immature bitter orange, 1-5 parts of rhizoma anemarrhenae, and 1-5 parts of cortex phellodendri.

3. A Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy according to claim 1, characterized in that: The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 6 parts of antler glue, 3 parts of astragalus, 3 parts of cinnamon twig, 3 parts of Panax notoginseng, 3 parts of Citrus aurantium, 3 parts of Anemarrhena asphodeloides, and 3 parts of Phellodendron chinense.

4. Use of the traditional Chinese medicine preparation according to any one of claims 1 to 3 for preparing a drug for preventing and treating bone marrow suppression after chemotherapy, characterized in that: The Chinese medicine preparation is prepared into granules, powders, oral liquids, pills, decoctions, freeze-dried powders or other pharmaceutically acceptable oral dosage forms according to conventional pharmaceutical preparation methods in pharmacy, with medically acceptable excipients.

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