Filling Equipment for an Efficient Amplification Kit

Abstract

The present invention relates to the technical field of biological experimental equipment, and provides a filling device for an efficient amplification kit, which includes a base; a positioning frame is located above the base, and the positioning frame is arranged to move up and down relative to the base. The positioning frame has a placement groove for accommodating the kit, and the bottom end of the kit abuts against the base; a liquid storage member is located above the positioning frame, and the liquid storage member is arranged to move up and down relative to the positioning frame. The liquid storage member has a sub-liquid storage cavity, and a vertical slideway is provided at the bottom of the sub-liquid storage cavity, and the vertical slideway communicates with the sub-liquid storage cavity; a liquid outlet head is slidably arranged in the vertical slideway, and the liquid outlet head has a liquid outlet flow channel with a liquid flow inlet and a liquid flow outlet. The liquid outlet head is configured such that after lifting and sliding, the liquid outlet flow channel communicates with or cancels communication with the sub-liquid storage cavity through the liquid flow inlet. Through the above technical solution, the problem in the prior art that when the reserve quantity of the kit is insufficient during actual experimental operations, the immediate experimental requirements cannot be met is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of biological experimental equipment, and specifically, to a filling device for a high-efficiency amplification kit. Background Art

[0002] A high-efficiency amplification kit is an important tool that plays a key role in the field of biomedicine, especially in the research and clinical application of immunocyte therapy. This kit is mainly used to promote and enhance the amplification of natural killer cells, thereby providing strong support for related disease treatment and immune research. During actual use, specific cytokine-based solutions and basal media need to be precisely filled into the kit.

[0003] Usually, laboratories and medical institutions purchase batch-filled kits for use. However, during actual experimental operations, some problems often occur. Due to the complexity and uncertainty of experiments, there may be a situation where the spare quantity is insufficient and cannot meet the immediate experimental needs. In addition, different experiments may have special requirements for the substance composition or content of the solutions inside the kit, and it is difficult for batch-produced filled kits to flexibly respond to these changes. When the substance or content of the internal solution needs to be changed, it is difficult to quickly obtain a batch of kits that meet specific requirements, which to a certain extent affects the progress and effect of the experiment. In view of the above situation, there is an urgent need for a filling device that can stably, cleanly, and quantitatively perform temporary filling for high-efficiency amplification kits when needed, so as to meet the diversity and immediacy requirements of experiments and improve the efficiency and flexibility of experiments. Summary of the Invention

[0004] The present invention provides a filling device for a high-efficiency amplification kit, which solves the problem in the related art that when the spare quantity of the kit is insufficient during actual experimental operations, the immediate experimental needs cannot be met.

[0005] The technical solution of the present invention is as follows:

[0006] A filling device for a high-efficiency amplification kit, used for filling the inside of the kit, includes:

[0007] A base;

[0008] A positioning frame, the positioning frame is located above the base, the positioning frame is arranged to move up and down relative to the base, the positioning frame has a placement groove, the placement groove is used to accommodate the kit, and the bottom end of the kit abuts against the base;

[0009] A liquid storage member, the liquid storage member is located above the positioning frame, the liquid storage member is arranged to move up and down relative to the positioning frame, the liquid storage member has a sub-liquid storage cavity, and a vertical slideway is provided at the bottom of the sub-liquid storage cavity, and the vertical slideway communicates with the sub-liquid storage cavity;

[0010] A liquid outlet head, which is slidably arranged in a vertical slideway. The liquid outlet head has a liquid outlet flow channel, and the liquid outlet flow channel has a liquid flow inlet and a liquid flow outlet. The liquid outlet head is configured such that after lifting and sliding, the liquid outlet flow channel communicates or cancels communication with the sub-liquid storage cavity through the liquid flow inlet.

[0011] As a further technical solution, the top end of the liquid outlet head has a blocking part, and the liquid flow inlet is located on one side of the blocking part. After the liquid outlet head slides down, the blocking part blocks the vertical slideway. The liquid outlet head also has a pushed part. After the liquid storage member drives the liquid outlet head to slide down, the liquid flow inlet is used to extend into the interior of the reagent kit, and the pushed part abuts against the upper end of the reagent kit, driving the liquid outlet head to slide up, thereby canceling the blocking of the vertical slideway by the blocking part, and the liquid outlet flow channel communicates with the sub-liquid storage cavity through the liquid flow inlet.

[0012] As a further technical solution, the sub-liquid storage cavity has a sub-inlet, and further includes:

[0013] A liquid separation disk, which is arranged in the liquid storage member. The liquid separation disk has a main liquid storage cavity, and the main liquid storage cavity has a main inlet and a liquid dispensing port;

[0014] A quantitative disk, which is rotatably arranged in the sub-liquid storage cavity. The quantitative disk has a liquid transfer through groove. The liquid dispensing port and the sub-liquid storage cavity are arranged out of phase in the circumferential direction. The quantitative disk is configured such that after rotation, the liquid transfer through groove communicates with the liquid dispensing port and the sub-liquid storage cavity in sequence.

[0015] As a further technical solution, the side wall of the placement groove is provided with horizontal slideways, and the horizontal slideways are symmetrically arranged on both sides of the placement groove. Further includes:

[0016] Two first clamping members, which are respectively slidably arranged in the horizontal slideways, and the first clamping members are configured to move closer to or away from each other after sliding. After moving closer to each other, the two first clamping members respectively abut against the side walls on both sides of the reagent kit.

[0017] As a further technical solution, it further includes an anti-deviation bracket, which has a vertical placement space for placing the reagent kit. The anti-deviation bracket is arranged on the base, and the anti-deviation bracket has a guiding inclined groove along the vertical direction. Further includes:

[0018] A second clamping member, which is located below the first clamping member. The second clamping member has a sliding part, and the sliding part is slidably arranged in the guiding inclined groove. The guiding inclined groove and the second clamping member are arranged in pairs, and the two second clamping members are configured to move away from or close to each other after lifting and sliding.

[0019] As a further technical solution, the anti-deflection bracket further has a horizontally opened strip guide groove, and further comprises:

[0020] A first connecting rod, one end of which is hinged to the first clamping member, and the other end of which is hinged to the second clamping member, and the first connecting rod passes through and is slidably disposed in the strip guide groove, after the positioning frame slides down, the first connecting rod abuts against the side wall of the strip guide groove, and drives the first clamping member to swing toward the direction close to the reagent box.

[0021] As a further technical solution, it also includes:

[0022] A telescopic rod, one end of which is arranged on the base, and the other end is arranged on the liquid storage member, and the telescopic rod passes through the positioning frame, the telescopic rod has a stopper, and the stopper is located between the liquid storage member and the positioning frame, and the telescopic rod is configured so that after the liquid storage member slides down, the telescopic rod is driven to retract, and the stopper is brought into contact with the positioning frame, thereby driving the positioning frame to slide down synchronously.

[0023] As a further technical solution, it also includes:

[0024] A first elastic member, wherein one end of the first elastic member acts on the positioning frame, and the other end of the first elastic member acts on the liquid storage member to provide a force for the liquid storage member to move away from the positioning frame.

[0025] As a further technical solution, the anti-deflection bracket, the first clamp, the second clamp, and the first connecting rod together constitute a straightening assembly, and the straightening assembly is arranged in a circle. The placement groove, the sub-liquid storage chamber, the liquid distribution port and the liquid outlet head are all arranged in a circle, and the number of the two is the same as the straightening assembly.

[0026] As a further technical solution, the upper end of the liquid storage member has scale lines, and the number of the scale lines is the same as the number of the righting components, and further includes:

[0027] A rotating transmission member is arranged on the rotating shaft of the quantitative disk. The rotating transmission member has a pointer part, and the pointer part is aligned with or cancels the alignment with the scale line after rotation.

[0028] The working principle and beneficial effects of the present invention are:

[0029] In the present invention, the base is placed horizontally on the ground or a workbench, and the positioning frame is installed above the base through a guide column and a lifting drive device or manually, so as to realize lifting and moving relative to the base. The liquid storage part is installed above the positioning frame through another set of guide columns and a lifting structure. The liquid discharge head cooperates with the vertical slide and can slide up and down in the vertical slide. First, the reagent box to be filled is placed in the placement groove of the positioning frame. Then, the liquid storage part descends, driving the liquid discharge head to move together. When the liquid discharge head descends to the point where the liquid flow inlet of the liquid discharge channel is connected with the sub-liquid storage cavity, the liquid in the liquid storage cavity is poured into the reagent box through the liquid flow outlet of the liquid discharge channel. After the filling is completed, the liquid storage part rises, and the liquid discharge head rises accordingly, and the liquid flow inlet of the liquid discharge channel is disconnected from the sub-liquid storage cavity, and the filling is stopped. The filling amount is automatically controlled by the equipment, which avoids the errors that may occur in manual filling, improves the accuracy and consistency of filling, and provides convenience for subsequent experiments. The equipment has a compact structure and occupies a small space, which is convenient for use and placement in places with limited space such as laboratories. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The preferred implementation modes will be described below in a clear and understandable manner with reference to the accompanying drawings to further illustrate the above-mentioned characteristics, technical features, advantages and implementation methods of the present invention.

[0031] Figure 1 This is a schematic diagram of the overall external structure of the present invention;

[0032] Figure 2 It is another perspective structural schematic diagram of the present invention;

[0033] Figure 3 for Figure 2 Schematic diagram of the internal structure;

[0034] Figure 4 for Figure 3 A partial enlarged structural diagram of the middle part;

[0035] Figure 5 for Figure 2 A schematic diagram of the internal structure from another perspective;

[0036] Figure 6 It is a schematic diagram of the structure of the liquid outlet head in the present invention;

[0037] Figure 7 It is a schematic diagram of the structure of the righting assembly in the present invention.

[0038] In the figure: base - 1, positioning frame - 2, placement groove - 201, horizontal sliding groove - 202, liquid storage member - 3, sub - liquid storage cavity - 301, vertical sliding track - 302, sub - liquid inlet - 303, scale line - 304, liquid outlet head - 4, liquid outlet flow channel - 401, liquid flow inlet - 402, liquid flow outlet - 403, blocking part - 404, pushed part - 405, liquid distribution tray - 5, main liquid storage cavity - 501, main liquid inlet - 502, liquid preparation port - 503, metering tray - 6, liquid transfer through - groove - 601, first clamping member - 7, anti - deviation support - 8, centering assembly - 800, placement space - 801, guiding inclined groove - 802, strip - shaped guiding groove - 803, second clamping member - 9, sliding part - 901, first connecting rod - 10, telescopic rod - 11, blocking part - 1101, first elastic member - 12, rotational transmission member - 13, pointer part - 1301. Detailed implementation manners

[0039] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the specific implementation manners of the present invention will be described below with reference to the accompanying drawings. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings, and other implementation manners can also be obtained.

[0040] To make the drawings concise, only the parts related to the invention are schematically shown in each drawing, and they do not represent the actual structure of the product. In addition, to make the drawings concise and easy to understand, in some drawings, parts with the same structure or function are only schematically shown for one of them, or only one of them is marked. In this article, "one" not only means "only this one", but also means "more than one" situation, and "several" includes "two" and "more than two".

[0041] In this article, it should be noted that unless otherwise clearly specified and limited, the terms "installation", "connection", and "coupling" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0042] In addition, in the description of this application, the terms "first", "second", etc. are only used for distinguishing descriptions, and cannot be understood as indicating or implying relative importance.

[0043] Refer to Figures 1 to 7, the first embodiment of the present invention proposes a filling device for an efficient amplification kit, which is used to fill the inside of the kit, including a base 1; a positioning frame 2 is located above the base 1, and the positioning frame 2 is arranged to move up and down relative to the base 1. The positioning frame 2 has a placement groove 201, and the placement groove 201 is used to accommodate the kit, and the bottom end of the kit abuts against the base 1; a liquid storage member 3 is located above the positioning frame 2, and the liquid storage member 3 is arranged to move up and down relative to the positioning frame 2. The liquid storage member 3 has a sub-liquid storage cavity 301, and the bottom of the sub-liquid storage cavity 301 has a vertical slideway 302, and the vertical slideway 302 communicates with the sub-liquid storage cavity 301; a liquid outlet head 4 is slidably arranged in the vertical slideway 302, and the liquid outlet head 4 has a liquid outlet flow channel 401. The liquid outlet flow channel 401 has a liquid flow inlet 402 and a liquid flow outlet 403. The liquid outlet head 4 is configured such that after lifting and sliding, the liquid outlet flow channel 401 communicates with or cancels communication with the sub-liquid storage cavity 301 through the liquid flow inlet 402.

[0044] In this embodiment, the base 1 is horizontally placed on the ground or a workbench. The positioning frame 2 is installed above the base 1 through guide columns and a lifting drive device or manually, and can move up and down relative to the base 1. The liquid storage member 3 is installed above the positioning frame 2 through another set of guide columns and a lifting structure. The liquid outlet head 4 cooperates with the vertical slideway 302 and can slide up and down in the vertical slideway 302. First, place the kit to be filled in the placement groove 201 of the positioning frame 2. Then, the liquid storage member 3 descends, driving the liquid outlet head 4 to move together. When the liquid outlet head 4 descends to the point where the liquid flow inlet 402 of the liquid outlet flow channel 401 communicates with the sub-liquid storage cavity 301, the liquid in the liquid storage cavity 301 is poured into the kit through the liquid flow outlet 403 of the liquid outlet flow channel 401. After the filling is completed, the liquid storage member 3 rises, and the liquid outlet head 4 rises accordingly. The liquid flow inlet 402 of the liquid outlet flow channel 401 cancels communication with the sub-liquid storage cavity 301, and the filling stops. The filling volume is automatically controlled by the device, avoiding the errors that may occur in manual filling, improving the filling accuracy and consistency, and providing convenience for subsequent experiments. The device has a compact structure, occupies a small space, and is convenient to use and install in places with limited space such as laboratories.

[0045] Furthermore, the top end of the liquid outlet head 4 has a blocking portion 404, and the liquid flow inlet 402 is located on one side of the blocking portion 404. After the liquid outlet head 4 slides down, the blocking portion 404 blocks the vertical slideway 302. The liquid outlet head 4 also has a pushed portion 405. After the liquid storage member 3 drives the liquid outlet head 4 to slide down, the liquid flow inlet 402 is used to extend into the inside of the kit, and the pushed portion 405 abuts against the upper end of the kit, driving the liquid outlet head 4 to slide up, thereby causing the blocking portion 404 to cancel blocking the vertical slideway 302, and the liquid outlet flow channel 401 communicates with the sub-liquid storage cavity 301 through the liquid flow inlet 402.

[0046] In this embodiment, the liquid storage member 3 drives the liquid outlet head 4 to descend and slide. The liquid flow inlet 402 of the liquid outlet head 4 extends into the interior of the reagent kit. At this time, the pushed part 405 abuts against the upper end of the reagent kit. Due to the blockage of the reagent kit, the liquid outlet head 4 is driven to slide upward relative to it, so that the blocking part 404 no longer blocks the vertical slideway 302. The liquid outlet flow channel 401 is communicated with the sub-liquid storage cavity 301 through the liquid flow inlet 402, and the filling starts. The design of the blocking part 404 can effectively prevent liquid leakage when not filling, ensuring the sealing performance and safety of the device in the non-working state, and bringing convenience to the operation. The interaction between the pushed part 405 and the reagent kit realizes the automatic connection control of the liquid outlet head 4, without additional complex operations, improving the convenience and automation degree of filling. This automatic connection mechanism can accurately start filling when the reagent kit is in place, reducing the time and error of manual intervention and improving the filling efficiency. The trigger-type filling method only fills the workstations where the reagent kits are placed when the reagent kits placed on the device are not full, saving the liquid medicine, avoiding unnecessary waste, and at the same time meeting the needs of immediate use without a large amount of filling, increasing the flexibility and practicality of filling.

[0047] Furthermore, the sub-liquid storage cavity 301 has a sub-inlet 303, and further includes a liquid distribution tray 5. The liquid distribution tray 5 is arranged in the liquid storage member 3. The liquid distribution tray 5 has a main liquid storage cavity 501. The main liquid storage cavity 501 has a main inlet 502 and a liquid dispensing port 503. The quantitative tray 6 is rotatably arranged in the sub-liquid storage cavity 301. The quantitative tray 6 has a liquid transfer through groove 601. The liquid dispensing port 503 and the sub-liquid storage cavity 301 are arranged out of phase in the circumferential direction. The quantitative tray 6 is configured such that after rotation, the liquid transfer through groove 601 is sequentially communicated with the liquid dispensing port 503 and the sub-liquid storage cavity 301.

[0048] In this embodiment, liquid enters the main liquid storage chamber 501 from the main liquid inlet 502, and then flows out from the liquid dispensing port 503. The quantitative disk 6 rotates, and when the liquid transfer slot 601 rotates to align with the liquid dispensing port 503, the liquid enters the liquid transfer slot 601. As the quantitative disk 6 continues to rotate, the liquid transfer slot 601 is connected with the sub-liquid storage chamber 301, and the quantitative liquid is sent into the sub-liquid storage chamber 301. The capacity in the liquid transfer slot is fixed here, such as 2ml. The amount of solution transferred in each transfer process is fixed. Multiple quantitative injections can be achieved by the number of rotations of the quantitative disk. The cooperation of the liquid transfer disk 5 and the quantitative disk 6 greatly improves the quantitative accuracy of the filling, which is conducive to the standardization and repeatability of the experiment. The filling amount is controlled by the number of rotations of the quantitative disk 6. The operation is simple and intuitive, and it is easy to realize automatic control, which improves work efficiency. This fixed capacity and repeatable quantitative method effectively avoids the error of the filling amount, ensures the consistency of the amount of liquid medicine in each test kit, and provides a strong guarantee for the reliability of the experimental results. Due to the precise control of the filling volume, the waste of liquid medicine is reduced, the experimental cost is reduced, and it is more environmentally friendly. For different filling volume requirements, only the number of rotations of the quantitative disk 6 needs to be calculated and controlled, which increases the flexibility and adaptability of the equipment and can meet the filling requirements under various experimental conditions.

[0049] Furthermore, the side wall of the placement groove 201 is provided with a horizontal slide groove 202, which is symmetrically arranged on both sides of the placement groove 201, and also includes a first clamping member 7. There are two first clamping members 7, which are respectively slidably arranged in the horizontal slide groove 202, and the first clamping members 7 are configured to move closer to or away from each other after sliding. After moving closer to each other, the two first clamping members 7 respectively abut against the side walls on both sides of the test kit.

[0050] In this embodiment, the side walls of the placement groove 201 are symmetrically provided with horizontal slide grooves 202, and the two first clamps 7 are respectively installed in the horizontal slide grooves 202 on both sides and can slide therein. The first clamp 7 is a claw-shaped structure with certain elasticity and friction. After the reagent box is placed in the placement groove 201, the two first clamps 7 are pushed to slide in the horizontal slide groove 202, so that they are close to each other until they are closely abutted against the side walls on both sides of the reagent box, thereby fixing the reagent box in the center position of the placement groove 201. It is ensured that the reagent box will not be displaced in the horizontal direction during the filling process, the accuracy of the filling position is guaranteed, and the filling quality is improved. The operation is simple and convenient, and the reagent box can be fixed by manual pushing without complicated operation steps. The close abutment of the first clamp 7 with the side wall of the reagent box increases the friction force and further improves the stability of the reagent box. The symmetrically arranged structure makes the reagent box evenly stressed when fixed, avoiding tilting or damage caused by uneven stress. The reagent box can be quickly fixed, and the efficiency of filling is improved, which is particularly suitable for the scene of batch filling.

[0051] Further, it further includes an anti-deviation bracket 8. The anti-deviation bracket 8 has a vertical placement space 801 for placing the reagent kit. The anti-deviation bracket 8 is arranged on the base 1. The anti-deviation bracket 8 has a guiding inclined slot 802 along the vertical direction. It further includes a second clamping member 9. The second clamping member 9 is located below the first clamping member 7. The second clamping member 9 has a sliding portion 901. The sliding portion 901 is slidably arranged in the guiding inclined slot 802. The guiding inclined slot 802 and the second clamping member 9 are arranged in pairs. The two second clamping members 9 are configured to move away from or close to each other after lifting and sliding.

[0052] In this embodiment, after the reagent kit is placed in the placement space 801, as the positioning frame 2 descends, the first clamping member 7 drives the second clamping member 9 to descend together. Due to the effect of the guiding inclined slot 802, the sliding portions 901 of the two second clamping members 9 slide in the guiding inclined slot 802, causing the two second clamping members 9 to approach each other, thereby further fixing the reagent kit from below and preventing it from shifting during the filling process. The cooperation between the anti-deviation bracket 8 and the second clamping member 9 provides double-layer fixing and anti-deviation measures from top and bottom, greatly improving the stability and accuracy of the reagent kit during the filling process. The setting of the guiding inclined slot 802 converts the vertical movement into a horizontal approaching movement, realizing the automatic clamping of the lower part of the reagent kit without an additional driving device, simplifying the structure and operation. It can adapt to the dimensional and shape errors of the reagent kit. Through the adaptive movement of the second clamping member 9, it ensures effective anti-deviation fixing for reagent kits of various specifications. It effectively avoids the position shift of the reagent kit during the filling process due to vibration or other factors, ensuring the filling precision and quality.

[0053] Further, the anti-deviation bracket 8 further has a horizontally opened strip-shaped guiding slot 803. It further includes a first connecting rod 10. One end of the first connecting rod 10 is hinged to the first clamping member 7, and the other end is hinged to the second clamping member 9. And the first connecting rod 10 passes through and is slidably arranged in the strip-shaped guiding slot 803. After the positioning frame 2 descends and slides, the first connecting rod 10 abuts against the side wall of the strip-shaped guiding slot 803 and drives the first clamping member 7 to swing towards the direction close to the reagent kit.

[0054] In this embodiment, when the positioning frame 2 descends and slides, it drives the first clamping member 7 and the second clamping member 9 to descend. Since the first connecting rod 10 passes through the strip-shaped guiding slot 803, when it descends to a certain extent, the first connecting rod 10 abuts against the side wall of the strip-shaped guiding slot 803, thereby driving the first clamping member 7 to swing towards the direction close to the reagent kit, further strengthening the fixing of the reagent kit. Through the cooperation between the first connecting rod 10 and the strip-shaped guiding slot 803, it realizes that when the positioning frame 2 descends, it automatically drives the first clamping member 7 to swing and clamp the reagent kit, improving the automation degree and efficiency of the fixing. It can fix the reagent kit more tightly, especially gradually enhancing the fixing effect during the descent of the positioning frame 2, improving the stability of the reagent kit during the filling process. The structure is simple and reliable, reducing the possibility of failures and lowering the maintenance cost of the equipment.

[0055] Further, it further includes a telescopic rod 11. One end of the telescopic rod 11 is arranged on the base 1, and the other end is arranged on the liquid storage member 3. The telescopic rod 11 penetrates through the positioning frame 2. The telescopic rod 11 has a blocking portion 1101, and the blocking portion 1101 is located between the liquid storage member 3 and the positioning frame 2. The telescopic rod 11 is configured such that when the liquid storage member 3 slides down, it drives the telescopic rod 11 to contract, and makes the blocking portion 1101 abut against the positioning frame 2, driving the positioning frame 2 to slide down synchronously.

[0056] In this embodiment, when the liquid storage member 3 slides down, it drives the telescopic rod 11 to contract. When it contracts to a certain extent, the blocking portion 1101 abuts against the positioning frame 2, thereby driving the positioning frame 2 to slide down synchronously. The setting of the telescopic rod 11 realizes the linkage of the sliding actions of the liquid storage member 3 and the positioning frame 2, simplifies the control process of the equipment, and improves the convenience of operation. It ensures the synchronism of the sliding of the liquid storage member 3 and the positioning frame 2, makes the filling process more coordinated and stable, and improves the filling accuracy and quality. The telescopic rod 11 is set to be able to hover at any position. The design principle of this hoverable feature is based on the damping and locking mechanisms inside the telescopic rod 11. Through the internal damping structure, the telescopic speed of the telescopic rod 11 can be controlled, enabling it to perform telescopic actions smoothly. And the locking mechanism can provide sufficient resistance and supporting force when the telescopic rod 11 reaches any position, keeping it in the current position to achieve hovering.

[0057] Further, it further includes a first elastic member 12. One end of the first elastic member 12 acts on the positioning frame 2, and the other end acts on the liquid storage member 3, providing a force for the liquid storage member 3 to move away from the positioning frame 2.

[0058] In this embodiment, when the liquid storage member 3 descends for filling, it compresses the first elastic member 12 to store elastic potential energy. After filling is completed, the first elastic member 12 releases the elastic potential energy, providing a force for the liquid storage member 3 to move away from the positioning frame 2, causing the liquid storage member 3 to rise and reset. The setting of the first elastic member 12 provides automatic power for the rising and resetting of the liquid storage member 3, reduces additional driving devices, and simplifies the equipment structure and control process.

[0059] Further, the anti-deviation bracket 8, the first clamping member 7, the second clamping member 9, and the first connecting rod 10 together form a centering component 800. The centering component 800 is arranged in a plurality of circumferential arrangements. There are a plurality of placing grooves 201, liquid storage chambers 301, liquid distribution ports 503, and liquid outlet heads 4 arranged in a circumferential arrangement, and the number of each is the same as that of the centering component 800.

[0060] In this embodiment, during the filling process, multiple straightening assemblies 800 straighten and fix multiple test kits at the same time to ensure that the position of each test kit is accurate and stable. The various components work together to achieve the filling operation of multiple test kits at the same time. Despite the small-batch production, the provision of multiple circularly arranged straightening assemblies 800 can still significantly improve the filling efficiency, saving operation time compared to a single filling station. The circular arrangement design can still make the equipment relatively uniformly stressed during operation, reduce the deviation caused by local uneven stress, and ensure the accuracy and quality of small-batch filling. Processing multiple test kits at a time can better utilize the limited space and resources of the equipment under small-batch requirements and improve the comprehensive utilization efficiency of the equipment. The number and arrangement of each component are matched to ensure the relative synchronization and consistency of small-batch filling operations, so that the product quality is relatively uniform. It is convenient to carry out local modular maintenance. When individual straightening assemblies or filling stations fail, they can be repaired or replaced in a targeted manner without affecting the progress of the overall small-batch production.

[0061] Furthermore, the upper end of the liquid storage part 3 has scale lines 304, and the number of scale lines 304 is also the same as the number of the straightening components 800. It also includes a rotating transmission part 13, which is arranged on the rotating axis of the quantitative disk 6. The rotating transmission part 13 has a pointer part 1301, and the pointer part 1301 is aligned with the scale lines 304 or cancels the alignment after rotation.

[0062] In the present embodiment, when it is necessary to adjust the rotation angle of the liquid separation disk 5 to control the liquid separation amount, the rotating transmission member 13 can be driven manually or electrically to rotate. The rotating transmission member 13 drives the quantitative disk 6 to rotate so that the pointer portion 1301 is aligned with the corresponding scale line 304, thereby accurately setting the liquid separation amount. The cooperation of the scale line 304 and the pointer portion 1301 provides an intuitive reference for the adjustment of the liquid separation amount, which is convenient for the operator to quickly and accurately set the liquid separation amount according to the experimental requirements. The manual or electrically controlled driving mode increases the flexibility of the equipment and can adapt to the needs of different experimental scenes and operating habits. In small batch experiments, this precise liquid separation amount control helps to improve the repeatability and accuracy of the experiment and ensure the reliability of the experimental results.

[0063] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A filling device for a high-efficiency amplification kit, used for filling the inside of the kit, characterized in that: include: Base (1); a positioning frame (2), the positioning frame (2) being located above the base (1), the positioning frame (2) being arranged to be lifted and moved relative to the base (1), the positioning frame (2) having a placement groove (201), the placement groove (201) being used to accommodate the reagent kit, and the bottom end of the reagent kit abuts against the base (1); A liquid storage member (3), the liquid storage member (3) being located above the positioning frame (2), the liquid storage member (3) being arranged to be lifted and moved relative to the positioning frame (2), the liquid storage member (3) having a sub-liquid storage cavity (301), the bottom of the sub-liquid storage cavity (301) having a vertical slideway (302), the vertical slideway (302) being in communication with the sub-liquid storage cavity (301); a liquid outlet head (4), the liquid outlet head (4) being slidably disposed in a vertical slideway (302), the liquid outlet head (4) having a liquid outlet channel (401), the liquid outlet channel (401) having a liquid flow inlet (402) and a liquid flow outlet (403), the liquid outlet head (4) being configured such that after the liquid outlet head (4) is lifted and slid, the liquid outlet channel (401) is connected to or disconnected from the sub-liquid storage chamber (301) through the liquid flow inlet (402); The side wall of the placement groove (201) is provided with a horizontal slide groove (202), and the horizontal slide groove (202) is symmetrically arranged on both sides of the placement groove (201), and further comprises: First clamping members (7), the first clamping members (7) being two in number and respectively slidably disposed in the horizontal slide groove (202), and the first clamping members (7) being configured to move closer to or farther from each other after sliding, and when moving closer to each other, the two first clamping members (7) respectively abut against the side walls on both sides of the reagent box; An anti-deflection bracket (8), the anti-deflection bracket (8) having a vertical placement space (801), the placement space (801) being used to place the reagent kit, the anti-deflection bracket (8) being arranged on the base (1), the anti-deflection bracket (8) having a vertical guiding inclined groove (802), and further comprising: a second clamping member (9), the second clamping member (9) being located below the first clamping member (7), the second clamping member (9) having a sliding portion (901), the sliding portion (901) being slidably arranged in the guide inclined groove (802), the guide inclined groove (802) and the second clamping member (9) being arranged in pairs, and the two second clamping members (9) being configured to move away from or approach each other after being lifted and slid; A telescopic rod (11), one end of the telescopic rod (11) being arranged on the base (1), and the other end of the telescopic rod (11) being arranged on the liquid storage member (3), and the telescopic rod (11) passing through the positioning frame (2), the telescopic rod (11) having a stopper (1101), the stopper (1101) being located between the liquid storage member (3) and the positioning frame (2), the telescopic rod (11) being configured such that, after the liquid storage member (3) slides down, the telescopic rod (11) is driven to retract, and the stopper (1101) is brought into contact with the positioning frame (2), thereby driving the positioning frame (2) to slide down synchronously.

2. The filling device of the high-efficiency amplification kit according to claim 1, characterized in that: The top of the liquid outlet head (4) has a blocking portion (404), and the liquid flow inlet (402) is located on one side of the blocking portion (404). After the liquid outlet head (4) slides down, the blocking portion (404) blocks the vertical slideway (302). The liquid outlet head (4) also has a pushed portion (405). After the liquid storage member (3) drives the liquid outlet head (4) to slide downward, the liquid flow inlet (402) is used to extend into the interior of the reagent box, and the pushed portion (405) abuts against the upper end of the reagent box, driving the liquid outlet head (4) to slide upward, thereby causing the blocking portion (404) to stop blocking the vertical slideway (302), and the liquid outlet flow channel (401) is connected to the sub-liquid storage chamber (301) through the liquid flow inlet (402).

3. The filling device of the high-efficiency amplification kit according to claim 1, characterized in that: The sub-liquid storage chamber (301) has a sub-liquid inlet (303) and further comprises: A liquid separation plate (5), the liquid separation plate (5) being arranged in the liquid storage element (3), the liquid separation plate (5) having a main liquid storage cavity (501), the main liquid storage cavity (501) having a main liquid inlet (502) and a liquid distribution port (503); A quantitative disk (6), the quantitative disk (6) being rotatably disposed in the sub-liquid storage chamber (301), the quantitative disk (6) having a liquid transfer groove (601), the liquid dispensing port (503) and the sub-liquid storage chamber (301) being staggered in the circumferential direction, and the quantitative disk (6) being configured such that after rotation, the liquid transfer groove (601) is in sequential communication with the liquid dispensing port (503) and the sub-liquid storage chamber (301).

4. The filling device of the high-efficiency amplification kit according to claim 3, characterized in that: The anti-deflection bracket (8) further comprises a horizontally arranged strip-shaped guide groove (803), and further comprises: A first connecting rod (10), one end of the first connecting rod (10) is hinged to the first clamping member (7), and the other end is hinged to the second clamping member (9), and the first connecting rod (10) passes through and is slidably arranged in the strip guide groove (803), after the positioning frame (2) slides downward, the first connecting rod (10) abuts against the side wall of the strip guide groove (803), and drives the first clamping member (7) to swing in a direction close to the reagent box.

5. The filling device of the high-efficiency amplification kit according to claim 4, characterized in that: Also includes: A first elastic member (12), wherein one end of the first elastic member (12) acts on the positioning frame (2) and the other end of the first elastic member (12) acts on the liquid storage member (3) to provide a force for moving the liquid storage member (3) away from the positioning frame (2).

6. The filling equipment of the high-efficiency amplification kit according to claim 5, characterized in that: The anti-deflection bracket (8), the first clamping member (7), the second clamping member (9), and the first connecting rod (10) together constitute a straightening assembly (800), and the straightening assembly (800) is arranged in a plurality of circles. The placement groove (201), the sub-liquid storage chamber (301), the liquid distribution port (503), and the liquid outlet head (4) are all arranged in a plurality of circles, and the number of the plurality of the straightening assemblies (800) is the same as that of the straightening assemblies (800).

7. The filling device of the high-efficiency amplification kit according to claim 6, characterized in that: The upper end of the liquid storage member (3) has scale lines (304), the number of the scale lines (304) is the same as the number of the righting assemblies (800), and further comprises: A rotating transmission member (13), the rotating transmission member (13) being arranged on a rotating shaft of the quantitative disk (6), the rotating transmission member (13) having a pointer portion (1301), the pointer portion (1301) being aligned with or de-aligned with the scale line (304) after rotation.

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