A Bacillus cereus vomitoxin separation device and culture method

Abstract

The present invention relates to the technical field of Bacillus cereus, and particularly relates to a separation device and a culture method for Bacillus cereus vomitoxin, including a culture hopper, with support columns arranged at the four corners of the bottom end of the culture hopper, and a cover plate arranged at the top of the culture hopper; a culture assembly, which is arranged inside the culture hopper and the cover plate; a separation assembly, which is arranged below the culture hopper; a gap opening and closing assembly, which is arranged between the culture hopper and the separation assembly, and the gap opening and closing assembly is used to send the preliminarily separated Bacillus cereus vomitoxin into the separation assembly; compared with the prior art, by setting a culture assembly, the present invention avoids the phenomenon of liquid culture medium stratification or precipitation. The preliminary screening module preliminarily separates the produced vomitoxin during the stirring process, effectively separates the smaller particle toxins from the bacteria, reduces the processing pressure of the subsequent separation assembly, and improves the separation efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of Bacillus cereus, and particularly relates to a separation device and a cultivation method for Bacillus cereus vomitoxin. Background Art

[0002] Bacillus cereus vomitoxin is a toxic metabolite produced by Bacillus cereus under specific environmental conditions, and its main component is a cyclic peptide compound (such as lactobacillin H1); this toxin is highly toxic to humans and can cause severe gastrointestinal discomfort, such as vomiting, abdominal pain, diarrhea, etc., and may even trigger food poisoning incidents; since Bacillus cereus is widely present in natural soil, food, and processing environments, the risk of food contamination is relatively high, especially starchy foods such as rice and flour products are prone to becoming a breeding ground for toxins; the main purpose of separating vomitoxin is to study its toxicity mechanism and detection methods, provide data support for food safety risk assessment, and at the same time is a key step in developing effective technologies for detecting and removing toxins, so as to prevent it from harming the food industry and public health.

[0003] In the prior art, in the process of separating Bacillus cereus vomitoxin, the traditional method usually directly mixes the culture medium and the Bacillus cereus inoculum for separation. However, this direct separation method often leads to difficulty in separating the toxin from the bacterial cells, thereby affecting the separation efficiency. On the one hand, the bacterial cells of Bacillus cereus and the generated vomitoxin often mix together, and traditional separation devices often lack an effective mechanism to distinguish the two, resulting in low efficiency in the subsequent separation process. In addition, due to the differences in the size and density of toxin particles and bacterial cells, the traditional method cannot quickly and effectively separate them, easily causing incomplete separation and even affecting the stability of the culture environment. Therefore, the present invention discloses a separation device and a cultivation method for Bacillus cereus vomitoxin to meet the stability of vomitoxin separation. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a separation device for Bacillus cereus vomitoxin to solve the problems that due to the differences in the size and density of toxin particles and bacterial cells, the traditional method cannot quickly and effectively separate them, easily causing incomplete separation and even affecting the stability of the culture environment.

[0005] Based on the above purpose, the present invention provides a separation device and a cultivation method for Bacillus cereus vomitoxin, including a cultivation hopper. Support columns are arranged at the four corners of the bottom end of the cultivation hopper. A cover plate is arranged at the top of the cultivation hopper. A feed inlet is opened on one side of the cover plate. A waste outlet opposite to the feed inlet is opened below one side of the cover plate. A through hole is opened in the middle of the bottom end of the cultivation hopper. Both the cultivation hopper and the cover plate are arranged in a convex shape. The cultivation hopper and the cover plate are arranged in an opposite direction.

[0006] A culture component, which is arranged inside the culture hopper and the cover plate. The culture component includes a stirring module and a preliminary screening module. The stirring module is rotatably installed inside the culture hopper and the cover plate, and a first rotating motor is arranged on one side of the culture hopper for driving the stirring module to rotate. The stirring module is used for stirring during the cultivation of Bacillus cereus. The preliminary screening module is arranged on the outer surface of the stirring module, and the preliminary screening module is used for preliminarily separating the Bacillus cereus vomitoxin.

[0007] A separation component, which is arranged below the culture hopper. The separation component is used for further separating the preliminarily separated Bacillus cereus vomitoxin.

[0008] A gap opening and closing component, which is arranged between the culture hopper and the separation component. The gap opening and closing component is used for feeding the preliminarily separated Bacillus cereus vomitoxin into the separation component.

[0009] Preferably, the stirring module includes vertical frames fixedly installed on both sides of the inner bottom end of the culture hopper. The top surfaces of the two vertical frames are flush with the top surface of the culture hopper. Bearing seats are arranged at the tops of the vertical frames. A rotating shaft is rotatably installed on the top surfaces of the two vertical frames through the bearing seats. The output end of the first rotating motor is fixedly connected to one end of the rotating shaft, and a stirring wheel is sleeved on the outer surface of the rotating shaft.

[0010] Preferably, the preliminary screening module includes a plurality of screening hoppers arranged circumferentially and equidistantly on the outer surface of the stirring wheel. A plurality of screening meshes adapted to the screening hoppers are arranged on the outer surface of the stirring wheel. The screening hoppers are arranged in an inclined shape, and one side of the screening hopper is adapted to the waste outlet.

[0011] Preferably, a rotating rod is rotatably installed on one side of the cover plate close to the feed inlet. One end of the rotating rod is fixedly connected to an adjusting wheel. A first baffle and a second baffle are arranged on one side of the rotating rod. A guiding plate inclined downward is arranged on one side of the first baffle. The first baffle is located above the waste outlet, and the second baffle is adapted to the feed inlet.

[0012] Preferably, the separation component includes a separation cylinder fixedly installed below the culture hopper. A fixed cylinder is fixedly installed at the inner bottom of the separation cylinder. A filter cylinder is rotatably installed above the fixed cylinder. A bearing is arranged between the fixed cylinder and the filter cylinder. A reciprocating lead screw is rotatably installed in the middle of the separation cylinder. A second rotating motor is fixedly installed in the middle of the bottom end of the separation cylinder. The output end of the second rotating motor is fixedly connected to the reciprocating lead screw. A connecting seat is fixedly sleeved on one side of the reciprocating lead screw. The connecting seat is fixedly connected to the filter cylinder. A filter element is arranged inside the filter cylinder. A spiral stirring bar is also arranged inside the filter cylinder. The bottom end of the stirring bar is fixedly connected to the connecting seat.

[0013] Preferably, liquid outlets and slag outlets are respectively arranged on both sides of the bottom of the culture hopper. The liquid outlet penetrates through the bottom of the separation cylinder. The slag outlet penetrates through the liquid outlet and the bottom of the fixed cylinder.

[0014] Preferably, the gap opening and closing component includes a fixing plate fixedly installed between the culture hopper and the separation cylinder, and a rotating ring rotatably installed below the fixing plate. A material dropping opening is formed in the middle of the fixing plate. The material dropping opening is adapted to the through hole. A plurality of opening and closing plates are rotatably installed on the inner side of the bottom of the material dropping opening. A plurality of connecting plates are rotatably installed on one side of the rotating ring. One sides of the plurality of connecting plates are rotatably connected to one sides of the opening and closing plates. When the plurality of opening and closing plates approach each other, the material dropping opening is closed. When the plurality of opening and closing plates move away from each other, the material dropping opening is opened.

[0015] Preferably, a plurality of limiting grooves are equidistantly formed on the outer circumference of the rotating ring. A plurality of limiting posts slidably installed inside the limiting grooves are arranged at the bottom of the fixing plate.

[0016] Preferably, an extension rod is arranged on one side of the bottom surface of the rotating ring. A trigger block is arranged at the bottom of the extension rod. A moving sleeve is threadedly connected to the outer surface of the reciprocating lead screw. A first positioning rod and a second positioning rod are respectively arranged on both sides of the moving sleeve. First push blocks and second push blocks are respectively arranged at the tops of the first positioning rod and the second positioning rod. The height of the first push block is lower than that of the second push block. The top surface of the first push block is an inclined surface. The bottom surface of the second push block is an inclined surface.

[0017] The present invention also discloses a method for culturing Bacillus cereus emetic toxin, which is applied to the above-mentioned Bacillus cereus emetic toxin separation device, and includes the following steps:

[0018] S1: Add a culture medium and a Bacillus cereus inoculation solution into the culture hopper through the feed inlet;

[0019] S2: Start the first rotating motor to drive the stirring module to rotate, so as to evenly mix the culture medium and the bacteria, improve the dissolved oxygen distribution in the culture solution, and promote the rapid growth and toxin secretion of the bacteria.

[0020] S3: During the cultivation process, the preliminary screening module on the surface of the stirring module preliminarily separates the produced vomitoxin, separates the smaller particle toxins from the bacteria. During the preliminary screening process, the waste is discharged through the waste outlet to avoid affecting the subsequent separation steps.

[0021] S4: Monitor the parameters of the cultivation environment. When the cultivation reaches the set time, stop the operation of the stirring module and prepare for material transfer.

[0022] S5: Start the separation system. The reciprocating lead screw moves upward to drive the gap opening and closing component to open the material dropping port, and accurately convey the toxin-containing liquid separated by the preliminary screening module to the separation component. After the material transfer is completed, the reciprocating lead screw moves downward to drive the gap opening and closing component to reset, and the material dropping port closes to ensure the isolation state between the cultivation hopper and the separation cylinder.

[0023] S6: In the separation component, the filter cylinder and the filter element are used to further separate the liquid after preliminary screening. The filter element intercepts the solid waste residue and discharges it through the slag outlet. The liquid toxin extracts a high-purity solution through centrifugal filtration and flows out from the liquid outlet.

[0024] Advantages of the present invention:

[0025] 1. For this Bacillus cereus vomitoxin separation device and cultivation method, by setting up a cultivation component, through the synergistic effect of the stirring module and the preliminary screening module, it can effectively improve the cultivation efficiency of Bacillus cereus and the yield of vomitoxin. Driven by the first rotating motor, the stirring module ensures the uniform mixing of the culture medium and the bacteria, maintains the uniformity of the dissolved oxygen distribution, promotes the rapid growth and toxin secretion of the bacteria, and at the same time avoids the phenomenon of culture solution stratification or precipitation. During the stirring process, the preliminary screening module preliminarily separates the produced vomitoxin, effectively separates the smaller particle toxins from the bacteria, reduces the processing pressure of the subsequent separation component, and improves the separation efficiency. In addition, the preliminary screening module is connected to the waste outlet, which can timely discharge the metabolic waste and avoid affecting the stability of the cultivation environment and subsequent operations.

[0026] 2. The Bacillus cereus emetic toxin separation device and cultivation method achieve precise adjustment of the quantity and time of toxin and waste falling into the separation cylinder through the precise control of the gap opening and closing assembly in cooperation with the opening and closing assembly. The opening and closing of the material dropping port avoid excessive material accumulation or omission, ensure the airtight isolation state between the cultivation hopper and the separation cylinder, thereby preventing cross-contamination and material leakage. The gap opening and closing assembly drives the moving sleeve with a reciprocating screw rod, and through the cooperation of the push block and the trigger block, precisely controls the rotation angle of the rotating ring and the opening degree of the opening and closing plate, achieving highly controllable material dropping quantity. At the same time, the material dropping process is synchronized with the processing cycle of the separation assembly, improving the separation efficiency and the operation stability of the equipment. The separation assembly effectively separates the falling toxin and waste with a filter cylinder and a filter element. The liquid component is discharged through the filter element to the liquid outlet, while the solid waste residue is intercepted by the filter element and gradually discharged to the slag outlet, further reducing the risk of blockage and the waste of the culture solution. The spiral stirring bars rotate synchronously to clean the residual materials on the surface of the filter element, ensuring the continuous and efficient filtration process and avoiding the frequency of equipment shutdown for maintenance. The collaborative design of the gap opening and closing assembly and the separation assembly not only improves the accuracy and efficiency of the material separation process but also optimizes the automation level and operation safety of the system, significantly enhancing the performance and production efficiency of the overall equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only those of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0028] Figure 1 Schematic diagram of the three-dimensional structure of the present invention;

[0029] Figure 2 Schematic diagram of the internal structure of the present invention;

[0030] Figure 3 Schematic diagram of the partial sectional structure of the present invention;

[0031] Figure 4 Schematic diagram of the internal structure of the present invention;

[0032] Figure 5 Schematic diagram of the stirring module and the primary screening module structure of the present invention;

[0033] Figure 6 Schematic diagram of the separation assembly structure of the present invention;

[0034] Figure 7 Schematic diagram of the bottom structure of the separation assembly of the present invention;

[0035] Figure 8 Schematic diagram of the internal sectional structure of the separation component of the present invention;

[0036] Figure 9 For the present invention Figure 8 Schematic diagram of the enlarged structure at position A in the present invention;

[0037] Figure 10 Schematic diagram of the internal structure of the filter cartridge of the present invention;

[0038] Figure 11 Schematic diagram of the three-dimensional structure of the gap opening and closing component of the present invention;

[0039] Figure 12 Schematic diagram of the planar structure of the gap opening and closing component of the present invention.

[0040] The markings in the figure are:

[0041] 1. Culture hopper; 2. Support column; 3. Cover plate; 4. Feed inlet; 5. Through hole; 6. Stand; 7. Rotating shaft; 8. Stirring wheel; 9. First rotating motor; 10. Screening hopper; 11. Screening mesh; 12. Rotating rod; 13. Adjusting wheel; 14. First baffle; 15. Second baffle; 16. Guide plate; 17. Waste outlet; 18. Separation cylinder; 19. Fixed plate; 20. Material dropping port; 21. Liquid outlet; 22. Fixed cylinder; 23. Filter cartridge; 24. Reciprocating lead screw; 25. Second rotating motor; 26. Connecting seat; 27. Filter element; 28. Stirring bar; 29. Rotating ring; 30. Opening and closing plate; 31. Connecting plate; 32. Limiting groove; 33. Limiting post; 34. Extension rod; 35. Trigger block; 36. Moving sleeve; 37. First positioning rod; 38. First push block; 39. Second positioning rod; 40. Second push block; 41. Residue outlet. Detailed implementation manners

[0042] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below in conjunction with specific embodiments.

[0043] It should be noted that unless otherwise defined, the technical terms or scientific terms used in the present invention should have the ordinary meanings understood by those with ordinary skills in the field to which the present invention belongs. The "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Words such as "including" or "comprising" mean that the elements or objects appearing before the word cover the elements or objects listed after the word and their equivalents, without excluding other elements or objects. Words such as "connected" or "coupled" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Upper", "lower", "left", "right", etc. are only used to represent relative position relationships. When the absolute position of the object being described changes, the relative position relationship may also change accordingly.

[0044] As Figures 1 to 12 shown, the Bacillus cereus emetic toxin separation device includes a culture hopper 1. Support columns 2 are provided at the four corners of the bottom end of the culture hopper 1. A cover plate 3 is provided at the top of the culture hopper 1. A feed port 4 is opened on one side of the cover plate 3. A waste outlet 17 opposite to the feed port 4 is opened below one side of the cover plate 3. A through hole 5 is opened in the middle of the bottom end of the culture hopper 1. Both the culture hopper 1 and the cover plate 3 are in a convex shape and are arranged oppositely; a culture assembly, the culture assembly is arranged inside the culture hopper 1 and the cover plate 3, the culture assembly includes a stirring module and a primary screening module. The stirring module is rotatably installed inside the culture hopper 1 and the cover plate 3, and a first rotating motor 9 is provided on one side of the culture hopper 1 for driving the stirring module to rotate. The stirring module is used for stirring during the cultivation of Bacillus cereus. The primary screening module is arranged on the outer surface of the stirring module and is used for the primary separation of Bacillus cereus emetic toxin; a separation assembly, the separation assembly is arranged below the culture hopper 1 and is used for further separating the preliminarily separated Bacillus cereus emetic toxin; a gap opening and closing assembly, the gap opening and closing assembly is arranged between the culture hopper 1 and the separation assembly and is used for feeding the preliminarily separated Bacillus cereus emetic toxin into the separation assembly;

[0045] The culture medium and the Bacillus cereus inoculum are added into the interior of the culture hopper 1 through the feed inlet 4. The first rotation motor 9 is started to drive the stirring module to rotate, so that the culture medium and the bacteria are evenly mixed, while ensuring uniform distribution of dissolved oxygen in the culture solution, promoting rapid growth of the bacteria and toxin secretion. The preliminary screening module on the surface of the stirring module preliminarily separates the produced vomitoxin during rotation, separating the smaller particle toxins from the bacteria. When the culture reaches the set time, the gap opening and closing assembly is opened to accurately transport the toxin-containing liquid separated by the preliminary screening module to the separating assembly below. The separating assembly further processes the preliminary screening product, and extracts a high-purity vomitoxin solution through centrifugal filtration. Among them, the culture hopper 1 and the cover plate 3 are arranged opposite to each other in a convex shape, ensuring the maximum internal space and reducing the possibility of external contamination at the same time.

[0046] As Figures 2 to 5 shown, the stirring module includes vertical frames 6 fixedly installed on both sides of the bottom end inside the culture hopper 1. The top surfaces of the two vertical frames 6 are flush with the top surface of the culture hopper 1. Bearing seats are provided at the top ends of the vertical frames 6. A rotating shaft 7 is rotatably installed on the top surfaces of the two vertical frames 6 through the bearing seats. The output end of the first rotation motor 9 is fixedly connected to one end of the rotating shaft 7. A stirring wheel 8 is sleeved on the outer surface of the rotating shaft 7. The preliminary screening module includes a number of screening hoppers 10 arranged equidistantly in a circle on the outer surface of the stirring wheel 8. A number of screening meshes 11 adapted to the screening hoppers 10 are arranged on the outer surface of the stirring wheel 8. The screening hoppers 10 are arranged in an inclined shape, and one side of the screening hopper 10 is adapted to the waste outlet 17;

[0047] The culture medium and the Bacillus cereus inoculum are added into the interior of the culture hopper 1 through the feed inlet 4. The first rotation motor 9 is started to drive the stirring module to rotate, so that the culture medium and the bacteria are evenly mixed, while ensuring uniform distribution of dissolved oxygen in the culture solution, promoting rapid growth of the bacteria and toxin secretion. The preliminary screening module on the surface of the stirring module preliminarily separates the produced vomitoxin during rotation, separating the smaller particle toxins from the bacteria. When the culture reaches the set time, the gap opening and closing assembly is opened to accurately transport the toxin-containing liquid separated by the preliminary screening module to the separating assembly below. The separating assembly further processes the preliminary screening product, and extracts a high-purity vomitoxin solution through centrifugal filtration. Among them, the waste produced during the preliminary screening process (such as bacterial metabolites or unnecessary culture medium residues) is discharged from the waste outlet 17 to avoid contaminating the subsequent separation steps. The culture hopper 1 and the cover plate 3 are arranged opposite to each other in a convex shape, ensuring the maximum internal space and reducing the possibility of external contamination at the same time. The stirring module is driven by the first rotation motor 9, which can realize uniform distribution of nutrients and bacteria during the culture process, enhance the growth rate of Bacillus cereus and the yield of vomitoxin, and at the same time avoid layering or precipitation of the culture solution;

[0048] A rotating rod 12 is rotatably installed on one side of the cover plate 3 close to the feed inlet 4. One end of the rotating rod 12 is fixedly connected to an adjusting wheel 13. A first baffle 14 and a second baffle 15 are arranged on one side of the rotating rod 12. A downwardly inclined guide plate 16 is arranged on one side of the first baffle 14. The first baffle 14 is located above the waste outlet 17. The second baffle 15 is adapted to the feed inlet 4;

[0049] When feeding is required, rotate the adjusting wheel 13 to drive the rotating rod 12 to rotate, so that the first baffle 14 enters the interior and rear of the waste outlet 17, completely closing the waste outlet 17. At the same time, move the second baffle 15 above the waste outlet 17 for shielding, thereby realizing the feeding operation; after the cultivation process is completed and the waste after primary screening needs to be discharged, continue to rotate the adjusting wheel 13. The rotating rod 12 drives the second baffle 15 to enter the interior and rear of the waste outlet 17, opening the waste outlet 17. At the same time, the first baffle 14 returns to its normal position. The downwardly inclined guide plate 16 guides the waste to smoothly discharge from the waste outlet 17. Through the cooperation of the first baffle 14 and the second baffle 15, the functions of feeding and waste discharge are separated, avoiding waste leakage or pollution of the cultivation environment during feeding, improving the airtightness and safety of cultivation. And the first baffle 14 is provided with a downwardly inclined guide plate 16, which improves the feeding efficiency during feeding and prevents materials from directly entering the waste outlet 17.

[0050] As Figures 6 to 10 shown, the separation assembly includes a separation cylinder 18 fixedly installed below the cultivation hopper 1. A fixed cylinder 22 is fixedly installed at the bottom inside the separation cylinder 18. A filter cylinder 23 is rotatably installed above the fixed cylinder 22. A bearing is arranged between the fixed cylinder 22 and the filter cylinder 23. A reciprocating lead screw 24 is rotatably installed in the middle inside the separation cylinder 18. A second rotating motor 25 is fixedly installed in the middle at the bottom end of the separation cylinder 18. The output end of the second rotating motor 25 is fixedly connected to the reciprocating lead screw 24. A connecting seat 26 is fixedly sleeved on one side of the reciprocating lead screw 24. The connecting seat 26 is fixedly connected to the filter cylinder 23. A filter element 27 is arranged inside the filter cylinder 23. A spiral stirring bar 28 is also arranged inside the filter cylinder 23. The bottom end of the stirring bar 28 is fixedly connected to the connecting seat 26. Liquid outlets 21 and slag outlets 41 are respectively arranged on both sides of the bottom of the cultivation hopper 1. The liquid outlet 21 penetrates through the bottom of the separation cylinder 18. The slag outlet 41 penetrates through the liquid outlet 21 and the bottom of the fixed cylinder 22;

[0051] After the primary screening, the culture medium flows into the inside of the separation cylinder 18 through the through hole 5 at the bottom of the culture hopper 1; the reciprocating lead screw 24 in the separation cylinder 18 is driven by the second rotating motor 25 at the bottom. When the lead screw rotates, it drives the connecting seat 26 fixedly sleeved on the lead screw to move up and down. The connecting seat 26 is fixedly connected to the filter cylinder 23, so that the filter cylinder 23 rotates in the separation cylinder 18. A filter element 27 is arranged inside the filter cylinder 23 to filter impurities in the culture medium. At the same time, the spiral stirring bars 28 inside the filter cylinder 23 also rotate synchronously driven by the rotation of the lead screw to stir and clean the residual materials on the surface of the filter element 27 to avoid blockage. After the culture medium is filtered by the filter element 27, the liquid component is discharged from the filter element 27 and flows out through the liquid outlet 21 at the bottom of the separation cylinder 18, while the solid waste residue is intercepted by the filter element 27 and gradually falls into the fixed cylinder 22, and gradually moves towards the slag outlet 41, and finally is discharged from the slag outlet 41 at the bottom of the separation cylinder 18. Gate valves are arranged at the pipelines of the liquid outlet 21 and the slag outlet 41 to control the opening and closing.

[0052] As Figure 8 , Figure 9 , Figure 11 , Figure 12 shown, the gap opening and closing assembly includes a fixed plate 19 fixedly installed between the culture hopper 1 and the separation cylinder 18, and a rotating ring 29 rotatably installed below the fixed plate 19. A material dropping port 20 is opened in the middle of the fixed plate 19. The material dropping port 20 is adapted to the through hole 5. A plurality of opening and closing plates 30 are rotatably installed on the inner side of the bottom of the material dropping port 20. A plurality of connecting plates 31 are rotatably installed on one side of the rotating ring 29. The plurality of connecting plates 31 are rotatably connected to one side of the opening and closing plates 30. When the plurality of opening and closing plates 30 approach each other, the material dropping port 20 is closed. When the plurality of opening and closing plates 30 move away from each other, the material dropping port 20 is opened. A plurality of limiting grooves 32 are equidistantly opened on the outer circumference of the rotating ring 29. A plurality of limiting columns 33 slidably installed inside the limiting grooves 32 are arranged at the bottom of the fixed plate 19. An extension rod 34 is arranged on one side of the bottom surface of the rotating ring 29. A trigger block 35 is arranged at the bottom of the extension rod 34. A moving sleeve 36 is threadedly connected to the outer surface of the reciprocating lead screw 24. A first positioning rod 37 and a second positioning rod 39 are respectively arranged on both sides of the moving sleeve 36. First push blocks 38 and second push blocks 40 are respectively arranged at the tops of the first positioning rod 37 and the second positioning rod 39. The height of the first push block 38 is lower than that of the second push block 40. The top surface of the first push block 38 is an inclined surface, and the bottom surface of the second push block 40 is an inclined surface;

[0053] The toxins and waste after primary screening are temporarily stored at the bottom of the culture hopper 1. When the reciprocating lead screw 24 is started, the gap opening and closing assembly is ready to open the discharge port 20. When the reciprocating lead screw 24 moves upward, the second push block first reaches below the trigger block 35, and its inclined bottom surface gradually pushes the trigger block 35 upward. The upward movement of the trigger block 35 drives the extension rod 34 to push the rotating ring 29 to rotate. The rotating ring 29 gradually opens several opening and closing plates 30 through the connecting plate 31, and the discharge port 20 is gradually opened. The toxins and waste start to enter the separation cylinder 18 from the discharge port 20. The opening degree of the opening and closing plates 30 is limited by the height of the push block and the angle of the rotating ring 29 to ensure accurate discharge volume. When the material falls into the separation cylinder 18, it is further separated by the filter cylinder 23 and the filter element 27. When the reciprocating lead screw 24 moves downward, after the set discharge volume is completed, the lead screw starts to move downward. The inclined top surface of the first push block 38 gradually contacts the trigger block 35, and the first push block 38 pushes the trigger block 35 downward to reset. The trigger block 35 drives the extension rod 34 to turn the rotating ring 29 back to its original position, and the connecting plate 31 gradually closes the opening and closing plates 30. After the opening and closing plates 30 are completely closed, the discharge port 20 is sealed to ensure the isolation state between the culture hopper 1 and the separation cylinder 18. Among them, the reciprocating lead screw 24 drives the moving sleeve 36 to move up and down. According to the height of the push block and the inclined surface of the trigger block 35, the opening and closing time and angle of the discharge port 20 can be accurately controlled, so as to control the discharge volume of the toxins. The separation assembly is linked with the gap opening and closing assembly to ensure that the falling time of the toxins is synchronized with the processing cycle of the separation assembly, avoiding excessive material accumulation or omission.

[0054] The present invention also discloses a method for culturing Bacillus cereus emetic toxin, which is applied to the above-mentioned Bacillus cereus emetic toxin separation device, and includes the following steps:

[0055] S1: Add the culture medium and Bacillus cereus inoculation solution into the interior of the culture hopper 1 through the feed port 4;

[0056] S2: Start the first rotating motor 9 to drive the stirring module to rotate, so that the culture medium and the bacteria are evenly mixed, improve the dissolved oxygen distribution in the culture solution, and promote the rapid growth and toxin secretion of the bacteria;

[0057] S3: During the culture process, the primary screening module on the surface of the stirring module preliminarily separates the produced emetic toxin, separates the smaller particle toxins from the bacteria. During the primary screening process, the waste is discharged through the waste outlet 17 to avoid affecting the subsequent separation steps;

[0058] S4: Monitor the parameters of the culture environment. When the culture reaches the set time, stop the operation of the stirring module and prepare for material transfer;

[0059] S5: Start the separation system. The reciprocating lead screw 24 moves upward, driving the gap opening and closing assembly to open the material dropping port 20, and accurately conveying the toxin-containing liquid separated by the primary screening module to the separation assembly. After the material conveying is completed, the reciprocating lead screw 24 moves downward, driving the gap opening and closing assembly to reset, and the material dropping port 20 is closed to ensure the isolation state between the culture hopper 1 and the separation cylinder 18.

[0060] S6: In the separation assembly, the filter cartridge 23 and the filter element 27 are used to further separate the liquid after primary screening. The filter element 27 intercepts solid waste residues and discharges them through the slag discharge port 41. The liquid toxin extracts a high-purity solution through centrifugal filtration and flows out from the liquid outlet 21.

[0061] The stirring module is used to uniformly mix the culture medium and the bacterial cells, and at the same time improve the distribution of dissolved oxygen, significantly accelerating the growth of the bacterial cells and the secretion of toxins, avoiding the occurrence of phenomena such as layering or precipitation of the culture solution, and improving the culture efficiency; the primary screening module preliminarily separates the toxins and the bacterial cells during the stirring process, timely discharges the metabolic waste, reduces the processing pressure of the subsequent separation assembly, and improves the overall separation efficiency; by accurately monitoring the culture conditions, ensuring that the bacterial cells are in the best growth environment, significantly increasing the toxin yield while ensuring the stability of the culture; and using the gap opening and closing assembly to accurately control the opening and closing time and the material dropping amount of the material dropping port 20, preventing material accumulation or omission, and at the same time maintaining the airtight isolation state between the culture hopper 1 and the separation cylinder 18, effectively avoiding cross-contamination.

[0062] Those of ordinary skill in the art should understand that: The discussion of any of the above embodiments is only exemplary and is not intended to imply that the scope of the present invention (including the claims) is limited to these examples; under the concept of the present invention, the technical features in the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations in different aspects of the present invention as described above, and they are not provided in detail for the sake of brevity.

[0063] The present invention aims to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A Bacillus cereus deoxynivalenol separation device, characterized in that: include: A culture bucket (1), wherein support columns (2) are arranged at four corners of the bottom end of the culture bucket (1), a cover plate (3) is arranged at the top of the culture bucket (1), a feed port (4) is arranged on one side of the cover plate (3), a waste outlet (17) opposite to the feed port (4) is arranged below one side of the cover plate (3), a through hole (5) is arranged in the middle of the bottom end of the culture bucket (1), the culture bucket (1) and the cover plate (3) are both arranged in a convex shape, and the culture bucket (1) and the cover plate (3) are arranged opposite to each other; A culture component, the culture component is arranged inside the culture bucket (1) and the cover plate (3), the culture component comprises a stirring module and a primary screening module, the stirring module is rotatably mounted inside the culture bucket (1) and the cover plate (3), and a first rotating motor (9) is arranged on one side of the culture bucket (1) for driving the stirring module to rotate, the stirring module is used to stir when culturing Bacillus cereus, the primary screening module is arranged on the outer surface of the stirring module, and the primary screening module is used to perform preliminary separation of Bacillus cereus vomitoxin; A separation component, the separation component is arranged below the culture bucket (1), and the separation component is used to further separate the initially separated Bacillus cereus vomitoxin; a gap opening and closing component, the gap opening and closing component being arranged between the culture bucket (1) and the separation component, and the gap opening and closing component being used to deliver the initially separated Bacillus cereus vomitoxin into the separation component; The stirring module comprises a stand (6) fixedly mounted on both sides of the bottom end of the culture bucket (1), the top surfaces of the two stands (6) are flush with the top surface of the culture bucket (1), the tops of the stands (6) are both provided with bearing seats, the top surfaces of the two stands (6) are provided with a rotating shaft (7) which is rotatably mounted together through the bearing seats, the output end of the first rotating motor (9) is fixedly connected to one end of the rotating shaft (7), the outer surface of the rotating shaft (7) is sleeved with a stirring wheel (8), the primary screening module comprises a plurality of screening buckets (10) equidistantly arranged on the outer surface of the stirring wheel (8), the outer surface of the stirring wheel (8) is provided with a plurality of screening nets (11) which are matched with the screening buckets (10), the screening buckets (10) are arranged in an inclined shape, and one side of the screening bucket (10) is matched with the waste outlet (17); The separation assembly comprises a separation cylinder (18) fixedly mounted below the culture bucket (1), a fixed cylinder (22) fixedly mounted on the inner bottom of the separation cylinder (18), a filter cylinder (23) rotatably mounted above the fixed cylinder (22), a bearing arranged between the fixed cylinder (22) and the filter cylinder (23), a reciprocating screw rod (24) rotatably mounted in the middle of the inner part of the separation cylinder (18), a second rotating motor (25) fixedly mounted in the middle of the bottom end of the separation cylinder (18), an output end of the second rotating motor (25) fixedly connected to the reciprocating screw rod (24), a connecting seat (26) fixedly sleeved on one side of the reciprocating screw rod (24), the connecting seat (26) fixedly connected to the filter cylinder (23), a filter element (27) arranged inside the filter cylinder (23), a spiral stirring bar (28) also arranged inside the filter cylinder (23), the bottom end of the stirring bar (28) fixedly connected to the connecting seat (26).

2. The Bacillus cereus deoxynivalenol separation device according to claim 1, characterized in that: A rotating rod (12) is rotatably mounted on one side of the cover plate (3) close to the feed port (4); one end of the rotating rod (12) is fixedly connected to an adjusting wheel (13); a first baffle plate (14) and a second baffle plate (15) are provided on one side of the rotating rod (12); a downwardly inclined guide plate (16) is provided on one side of the first baffle plate (14); the first baffle plate (14) is located above the waste material outlet (17); and the second baffle plate (15) is adapted to the feed port (4).

3. The Bacillus cereus deoxynivalenol separation device according to claim 2, characterized in that: A liquid outlet (21) and a slag outlet (41) are respectively arranged on both sides of the bottom of the culture bucket (1); the liquid outlet (21) is arranged through the bottom of the separation cylinder (18); and the slag outlet (41) is arranged through the liquid outlet (21) and the bottom of the fixed cylinder (22).

4. The Bacillus cereus deoxynivalenol separation device according to claim 3, characterized in that: The gap opening and closing assembly comprises a fixed plate (19) fixedly mounted between the culture bucket (1) and the separation cylinder (18), and a rotating ring (29) rotatably mounted below the fixed plate (19); a material drop opening (20) is provided in the middle of the fixed plate (19); the material drop opening (20) is matched with the through hole (5); a plurality of opening and closing plates (30) are rotatably mounted on the inner side of the bottom of the material drop opening (20); a plurality of connecting plates (31) are rotatably mounted on one side of the rotating ring (29); the plurality of connecting plates (31) are rotatably connected to one side of the opening and closing plate (30); when the plurality of opening and closing plates (30) are close to each other, the material drop opening (20) is closed; when the plurality of opening and closing plates (30) are away from each other, the material drop opening (20) is opened.

5. The Bacillus cereus deoxynivalenol separation device according to claim 4, characterized in that: A plurality of limiting grooves (32) are equidistantly formed on the outer circumference of the rotating ring (29), and a plurality of limiting posts (33) slidably mounted inside the limiting grooves (32) are provided at the bottom of the fixing plate (19).

6. The Bacillus cereus deoxynivalenol separation device according to claim 5, characterized in that: An extension rod (34) is provided on one side of the bottom surface of the rotating ring (29), a trigger block (35) is provided at the bottom of the extension rod (34), a moving sleeve (36) is threadedly connected to the outer surface of the reciprocating screw rod (24), a first positioning rod (37) and a second positioning rod (39) are provided on both sides of the moving sleeve (36), a first push block (38) and a second push block (40) are provided at the top ends of the first positioning rod (37) and the second positioning rod (39), the height of the first push block (38) is lower than the height of the second push block (40), the top surface of the first push block (38) is an inclined surface, and the bottom surface of the second push block (40) is an inclined surface.

7. A method for cultivating Bacillus cereus vomitoxin, applied to the Bacillus cereus vomitoxin separation device as claimed in claim 6, characterized in that: The following steps are involved: S1: adding culture medium and Bacillus cereus inoculum into the culture bucket (1) through the feed port (4); S2: starting the first rotating motor (9) to drive the stirring module to rotate, so that the culture medium and the bacteria are evenly mixed, the dissolved oxygen distribution in the culture solution is improved, and the rapid growth of the bacteria and the secretion of toxins are promoted; S3: During the culture process, the primary screening module on the surface of the stirring module performs preliminary separation of the produced vomitoxin, separating the smaller particles of toxin from the bacteria. During the primary screening process, the waste is discharged through the waste outlet (17) to avoid affecting the subsequent separation steps; S4: Monitor the parameters of the culture environment. When the culture reaches the set time, stop the operation of the stirring module and prepare for material transmission; S5: Start the separation system, the reciprocating screw (24) moves upward, drives the gap opening and closing assembly to open the material drop opening (20), and accurately delivers the toxin-containing liquid separated by the primary screening module to the separation assembly. When the material delivery is completed, the reciprocating screw (24) moves downward, drives the gap opening and closing assembly to reset, and closes the material drop opening (20), thereby ensuring the isolation state between the culture bucket (1) and the separation cylinder (18); S6: In the separation component, the liquid after the initial screening is further separated by using the filter cartridge (23) and the filter element (27). The filter element (27) intercepts the solid waste residue and discharges it through the residue outlet (41). The liquid toxin is extracted into a high-purity solution through centrifugal filtration and flows out from the liquid outlet (21).

Images