Anti-sugar moisturizing and brightening mask composition without irritation to eyes

Abstract

The invention discloses an anti-sugar moisturizing brightening mask composition without irritation to eyes. The anti-sugar moisturizing brightening mask composition comprises the following raw materials in percentage by weight: 92.99% of water; 2% of glycerin; 0.2% of Giga Moist PM-II hydrolyzed sclerotium gum, and 0.2% of Giga Moist PM-II hydrolyzed sclerotium gum; 0.03% of beta-glucan; 3% of 1, 2-propylene glycol; 0.1% of cosmetic grade sodium hyaluronate; 1% of PPG-24-glyceryl polyether-24; 0.1% of Xinhaidew acetyl chitosamine; 0.6% of Kesen K708; 0.05% of essence; according to the composition disclosed by the invention, the irritation test is carried out by using a cosmetic eye irritation-chick embryo chorioallantoic membrane vascular test method CAMVA method, and the detection method not only shortens the component irritation screening detection period of the composition, but also reduces the cost; meanwhile, a chick embryo chorioallantoic membrane blood vessel test method meets animal protection ethical requirements, the prepared composition finished product contains components capable of resisting saccharification and brightening skin, can be used by various skin types, can effectively reduce fine wrinkles and wrinkles and brighten the skin color, and finally has the effects of resisting wrinkles, preserving moisture and reducing dull skin for sensitive skin consumers.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to an eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition. Background Art

[0002] In the field of cosmetics, preservatives are one of the necessary ingredients to prevent product deterioration and extend shelf life. However, many preservatives show different degrees of irritation in eye irritation tests. For example, tests conducted by the chicken chorioallantoic membrane vascular test (CAMVA) method show that most preservatives score above 0, indicating that they are non-irritating, slightly irritating or severely irritating. Even if preservatives with a score of 0 are screened out, the adult white rabbit acute eye irritation / corrosion test method usually detects slight irritation.

[0003] Commercially available peptide silver or nanosilver preservatives are usually black turbid liquids or colorless transparent liquids, but silver preservatives have problems such as easy discoloration, easy precipitation, poor photostability, and sensitivity to ions and pH. In addition, the flavors and fragrances in cosmetics need to be increased in solubility by adding flavor solubilizers, and most commercially available flavor solubilizers are LRI solubilizers (PPG-26-butanol polyether-26, PEG-40 hydrogenated castor oil, water), CO40 solubilizers (PEG-40 hydrogenated castor oil), and NII-9 solubilizers (C12-13 chain alcohol polyether-9). These flavor solubilizers are mostly mildly irritating and moderately irritating in the HET-CAM eye irritation / corrosion test.

[0004] For commercially available facial masks, the irritation scoring method in the CAMVA method was used for testing. The results showed that most facial masks were non-irritating or slightly irritating, a few were moderately or severely irritating, and all test results scored above 0. This means that during use, if the mucous membrane is touched improperly, it may cause stinging. However, these mask ingredients are not suitable for all customers, and sensitive skin consumers have high skin sensitivity, so it is necessary to develop a non-irritating mask that enters the eyes to solve the market pain points. It is worth noting that 60% of the irritation in ordinary skin care products comes from preservatives and fragrance solubilizers.

[0005] According to the "Cosmetic Safety Technical Specifications 2015 Edition", skin irritation / corrosion tests and acute eye irritation / corrosion tests are all tested using animal methods, which brings about problems such as long testing cycles, high costs, and failure to meet animal protection ethical requirements. Therefore, most skin care products are not tested for irritation before they are put on the market. Most formulas on the market use anti-inflammatory agents to reduce the irritation of skin care products, which not only increases the cost of the formula, but also does not fundamentally solve the problem.

[0006] In response to the above problems, a new mask composition was developed, which is designed to be non-irritating to the eyes and has anti-sugar moisturizing and brightening effects on the skin. The development of this new mask composition is based on the recognition of the irritation problem of existing products and the attention to consumer needs. Summary of the invention

[0007] 1. Technical issues to be resolved In view of the deficiencies in the prior art, the present invention provides an eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition, which has the advantages of short detection cycle, low cost and the chicken embryo chorioallantoic membrane vascular test method complies with the ethical requirements of animal protection. It solves the ethical issues of using traditional facial masks for animal testing for irritation screening before animal experiments, the problem of insufficient irritation testing before skin care products are put on the market and the problem of high formula cost.

[0008] (II) Technical solution To achieve the above-mentioned purpose, the present invention provides the following technical scheme: an eye-irritating, anti-sugar, moisturizing and brightening mask composition, the composition comprising the following raw materials: water, glycerin, Giga Moist PM-II hydrolyzed sclerotium gum, β-glucan, 1,2-propylene glycol, cosmetic grade sodium hyaluronate, PPG-24-glycerol polyether-24, neohelu acetyl glucosamine, Kosen K708, flavor and flavor solubilizer.

[0009] Preferably, the weight proportions of the raw materials are: water 92.99%; glycerin 2%; Giga Moist PM-II hydrolyzed sclerotium gum 0.2%; β-glucan 0.03%; 1,2-propylene glycol 3%; cosmetic grade sodium hyaluronate 0.1%; PPG-24-glycerol polyether-24 1%; Neohexyl acetyl glucosamine 0.1%; Kosen K708 0.6%; flavor 0.05%; flavor solubilizer 0.15%.

[0010] Preferably, the composition is preliminarily screened for mildness of preservatives using a chicken embryo chorioallantoic membrane vascular test, and no vascular reaction is observed, and the CAM is normal, ie, the composition has mildness.

[0011] Preferably, the composition is subjected to sensory evaluation using a tabletop colorimeter, and the irritation of preservatives is tested. The eye irritation is detected by a test combination. The test method for the acute eye irritation / corrosion test of the composition in adult white rabbits is as follows: Gently pull open the lower eyelid of one eye of the rabbit, drop (or apply) 0.1 mL (100 mg) of the test substance into the conjunctival sac, and passively close the upper and lower eyelids for 1 second to prevent the loss of the test substance. The other eye is not treated as a self-control. The eyes are not rinsed within 24 hours after the test substance is dropped. If necessary, the eyes can be rinsed at 24 hours. Before the test, the cosmetic eye irritation-chicken embryo chorioallantoic membrane vascular test should be used for screening as non-irritating.

[0012] Preferably, the composition is compared with the appearance of the silver preservative according to the desktop colorimeter test method, the silver preservative is diluted to 1% with pure water, and the test method is to weigh 4-6g of the solution and place it in the detection chamber of the colorimeter, and connect the computer software to determine the specific color.

[0013] Preferably, the composition is tested for heat stability at 45 degrees Celsius according to the light stability in an ultraviolet weathering test box, the light stability in the ultraviolet weathering test box uses three 340 ultraviolet lamps, and the heat stability at 45 degrees Celsius uses a heat-resistant constant temperature control box as the testing equipment.

[0014] Preferably, the composition is tested for solubilizing performance and irritation performance.

[0015] Preferably, the solubilizer performance test is performed by adding 0.05% flavor, 0.15% solubilizer, and 99.8% pure water, mixing and stirring evenly, and comparing the transparency of the aqueous solution.

[0016] Preferably, the solubilizer irritation test is conducted by the irritation scoring method in the chicken chorioallantoic membrane test method for eye irritation / corrosion of cosmetics, according to the 2009 Entry-Exit Inspection and Quarantine Industry Standard of the People's Republic of China. When the test score is IS<1, it is evaluated as non-irritating, and the BLAUNON GT-100 fragrance solubilizer in the composition is evaluated as non-irritating.

[0017] Preferably, the composition is subjected to a facial mask irritation test, and the process is as follows: S1. Each test sample should be tested using the stimulation scoring method in 10 chicken embryos. Each batch of chicken embryos should have a negative control and a positive control, ≥ 3 each, and a blank solvent should be set up for control testing; S2, take out a chicken embryo of 10 days embryonic age, look after the air chamber with an egg candling device, determine the position of the air chamber, open a small window on the air chamber with a manual drilling tool with a hand-twisted drill, peel off the eggshell part on the upper strata of the air chamber with medical ophthalmic curved pointed tweezers, expose the white eggshell membrane, use a 1ml plastic pipette to drip an appropriate amount of 0.9% sodium chloride solution to moisten the eggshell membrane, pour out the 0.9% sodium chloride solution after completely moistening, and remove the eggshell membrane with tweezers, and the CAM is exposed after removing the eggshell membrane with abundant capillaries; S3. Place a polytetrafluoroethylene resin ring on the CAM as the test area, use a micropipette to transfer 40 µL of the sample to be tested into the polytetrafluoroethylene resin ring, record the time of adding the sample, wet the plastic wrap with sterile water and cover the wet surface of the plastic wrap on the air chamber, move the chicken embryo into a constant temperature and humidity chamber, and set the incubation time to 30 minutes; S4. Take out the tested chicken embryos, score the degree of vascular damage, use high-definition camera equipment to take photos and record the chorioallantoic membrane before and after the reaction, and analyze the damage and degree of CAM by comparing the photos before and after the reaction; S5. The results of each chicken embryo tested should be evaluated and scored. Finally, the highest and lowest scores will be removed from the 10 scores, leaving 8 valid scores, which are then averaged and used to score the irritation.

[0018] Compared with the prior art, the present invention provides an eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition, which has the following beneficial effects: 1. The eye-irritating, anti-sugar, moisturizing and brightening facial mask composition of the present invention is a cosmetic eye irritation-chicken embryo chorioallantoic membrane vascular test method CAMVA method, and the adult white rabbit acute eye irritation / corrosion test method in the 2015 version of the Cosmetic Safety Technical Specifications. The irritation test is carried out. This detection method not only shortens the irritant screening and detection cycle of the composition, but also reduces the cost. At the same time, the eye irritation-chicken embryo chorioallantoic membrane vascular test method meets the ethical requirements of animal protection, and solves the ethical issues of using animal tests for irritation screening before animal experiments on traditional facial masks, the problem of insufficient irritation testing before skin care products are put on the market, and the problem of high formula cost.

[0019] 2. The mask composition of the present invention has been tested and verified by the chicken embryo chorioallantoic membrane vascular test and the adult white rabbit acute eye irritation / corrosion test, and has the characteristics of being non-irritating, suitable for consumers with sensitive skin, and will not cause allergic reactions in consumers with sensitive skin. The raw materials of the composition - neohelu acetyl glucosamine and celloco β-glucan can effectively fight skin glycation, protect the skin from glycation damage, and enhance the effect of moisturizing and brightening the skin. Glycerin, 1,2-propylene glycol and cosmetic grade sodium hyaluronate ingredients have deep moisturizing effect on the skin, can lock moisture for a long time, and solve the problem of dryness. The composition uses (UNILUBE 5OTG-32) PPG-24-glyceryl polyether-24 and (BLAUNON GT-100) Fragrance solubilizer acts as a skin feel regulator, moisturizer and fragrance solubilizer, which helps reduce potential irritation or allergic reactions to the skin. The finished composition contains anti-glycation and skin brightening ingredients, which can be used by all skin types and can effectively reduce fine lines, wrinkles and brighten skin tone. Ultimately, it has anti-wrinkle, moisturizing and skin dullness reduction effects for consumers with sensitive skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a graph showing the eye irritation of the composition of the present invention - the irritation of the chicken embryo chorioallantoic membrane vascular test (normal saline); Figure 2 This is a graph showing the eye irritation of the composition of the present invention - the irritation of the chicken embryo chorioallantoic membrane vascular test (0.1 mol / L sodium hydroxide); Figure 3 This is a graph showing the eye irritation of the composition of the present invention - the irritation of the chicken embryo chorioallantoic membrane vascular test (pure water) test result; Figure 4 This is a graph showing the eye irritation of the composition of the present invention - the irritation test result of the chicken embryo chorioallantoic membrane vascular test; DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] See also Figure 1-Figure 4The composition includes the following raw materials: water, glycerin, Giga Moist PM-II hydrolyzed sclerotium gum, Cellec β-glucan, 1,2-propylene glycol (B), cosmetic grade sodium hyaluronate (1.3 million molecular weight), (UNILUBE5OTG-32) PPG-24-glyceryl polyether-24 (C), neohelu acetyl glucosamine, Kosen K708, fragrance (D) and (BLAUNONGT-100) fragrance solubilizer; The weight proportions of raw materials and their functions are: Water (92.99%): acts as a solvent to ensure that other ingredients are evenly distributed and applied to the skin, while providing basic hydration; Glycerin (2%): is a humectant that attracts moisture from the surrounding environment to the skin surface and helps maintain the skin's hydration layer; Giga Moist PM-II Hydrolyzed Sclerotium Gum (0.2%): This ingredient is used to improve the texture and stability of the product while also providing moisturizing effects; Cellec β-Glucan (0.03%): β-Glucan is a polysaccharide with antioxidant and skin-soothing properties; 1,2-Propanediol (3%): It is a humectant that helps maintain the stability of the product and helps dissolve other ingredients; Cosmetic grade sodium hyaluronate (0.1%): Hyaluronic acid is a naturally occurring polysaccharide that not only helps moisturize the skin and increase skin elasticity, but also attracts and locks in moisture; (UNILUBE 5OTG-32) PPG-24-glyceryl polyether-24 (1%): a skin feel regulator, moisturizer, and product skin feel improver used to reduce the stickiness in skin care products, enhance moisturizing properties, and improve product experience; Neohexyl Glucosamine (0.1%): Promotes the synthesis of hyaluronic acid in the human body, resists glycation, can help reduce skin dullness and increase skin elasticity; Kosen K708 (0.6%): is an antimicrobial agent containing silver, which has antibacterial and anti-inflammatory properties; Flavor (0.05%): used to provide fragrance and enhance user experience; (BLAUNON GT-100) Fragrance solubilizer (0.15%): It is a mixture of C12-14 polyether-12, PEG-30 hydrogenated castor oil, PEG-50 hydrogenated castor oil, and water. It is used to stabilize the formula so that the fragrance ingredients are evenly mixed in the formula without stratification.

[0023] The detailed proportions of the raw material components of the composition are shown in the following table:

[0024] Specifically, the composition was preliminarily screened for mildness of preservatives using the chicken embryo chorioallantoic membrane vascular test, and no vascular reaction was observed, and the CAM was normal, i.e., mildness (i.e., the same as the mask irritation test process). The test method was based on the irritation scoring method in the group standard T / GDCA 036-2024, Cosmetic Eye Irritation-Chicken Embryo Chorioallantoic Membrane Vascular Test Method. After the test was completed, the vascular damage degree score (see Table 1) and the average value were used to perform the irritation score (see Table 2).

[0025] Table 1 Vascular effects and scores

[0026] Table 2 Irritation rating

[0027] Table 3 Eye irritation of commercially available preservative cosmetics - Chicken embryo chorioallantoic membrane vascular test data

[0028] Specifically, the irritation of preservatives was tested by the acute eye irritation / corrosion test method for adult white rabbits in the 2015 version of the Cosmetic Safety Technical Specifications: Antimicro 909, Kosen K808, and Kosen K708 were initially screened out as 0 through the cosmetic eye irritation-chicken embryo chorioallantoic membrane vascular test method, which showed low irritation, and were further tested using the acute eye irritation / corrosion test method for white rabbits; Specifically, the composition is evaluated by a tabletop colorimeter test method, and the irritation of preservatives is tested. The eye irritation is tested by a test combination. The composition is tested according to the test method for acute eye irritation / corrosion test of adult white rabbits in the 2015 version of the Cosmetic Safety Technical Specifications: Gently pull open the lower eyelid of one eye of the rabbit, drop (or apply) 0.1 mL (100 mg) of the test substance into the conjunctival sac, and passively close the upper and lower eyelids for 1 second to prevent the loss of the test substance. The other eye is not treated as a self-control. Do not rinse the eye within 24 hours after the test substance is dropped. If necessary, rinse at 24 hours. Score according to Table 4, and screen for non-irritation using the cosmetic eye irritation-chicken embryo chorioallantoic membrane vascular test before testing.

[0029] Table 4 Acute eye irritation / corrosion test standard for adult white rabbits

[0030] Table 5 Test formulas of Antimicro 909, Kosen K808, and Kosen K708

[0031] Table 6 Test data of acute eye irritation / corrosion test of commercially available preservatives in adult white rabbits

[0032] Specifically, the composition is evaluated by a tabletop colorimeter test method, and the irritation of preservatives is tested. The eye irritation is tested by a test combination. The composition is tested according to the test method for acute eye irritation / corrosion test of adult white rabbits in the 2015 version of the Cosmetic Safety Technical Specifications: Gently pull open the lower eyelid of one eye of the rabbit, drop (or apply) 0.1 mL (100 mg) of the test substance into the conjunctival sac, and passively close the upper and lower eyelids for 1 second to prevent the loss of the test substance. The other eye is not treated as a self-control. Do not rinse the eye within 24 hours after the test substance is dropped. If necessary, rinse at 24 hours. Before testing, the cosmetic eye irritation-chicken embryo chorioallantoic membrane vascular test should be used for screening as non-irritating.

[0033] According to the data from the acute eye irritation / corrosion test on adult white rabbits, only silver preservatives are non-irritating in the formula, but some commercially available silver preservatives are black, which can cause discoloration in the formula and therefore need to be discarded.

[0034] Specifically, the composition is tested for light stability in an ultraviolet weathering test box and heat stability at 45 degrees Celsius. The light stability in the ultraviolet weathering test box uses three 340 ultraviolet lamps, and the heat stability at 45 degrees Celsius uses a heat-resistant constant temperature control box as the testing equipment.

[0035] Test method: dilute with water to the corresponding concentration, then use UV weathering test box and constant temperature box for testing, observe the color change, if the color change will affect the light stability of the formula, the more stable silver preservative should be screened out.

[0036] Table 7 Evaluation criteria for stability of silver preservatives

[0037] Table 8 Silver preservative light stability test

[0038] Table 9 Heat stability test of silver preservative at 45 degrees Celsius

[0039] Specifically, the composition was tested for solubilizing performance and irritation performance.

[0040] Specifically, the solubilizer performance test is performed as follows: add 0.05% flavor, 0.15% solubilizer, and 99.8% pure water, mix and stir evenly, and compare the transparency of the aqueous solution.

[0041] Specifically, the solubilizer irritation test is conducted according to the irritation scoring method in the 2009 Entry-Exit Inspection and Quarantine Industry Standard of the People's Republic of China, the chicken chorioallantoic membrane test method for eye irritation / corrosion of cosmetics. When the test score is IS < 1, it is evaluated as non-irritating, and the BLAUNON GT-100 fragrance solubilizer in the composition is evaluated as non-irritating.

[0042] (1) Use 9-day-old chicken embryos for egg candling and mark the location of the air chamber on the surface of the eggshell; use dental serrated curved forceps to peel off the marked portion of the eggshell to expose the white egg membrane, and be careful not to damage the integrity of the egg membrane. Use a pipette to add a few mL of 0.9% sodium chloride (Nacl) solution to moisten the egg membrane. At this time, you can proceed to the next step immediately. Otherwise, the chicken embryo should be placed in an incubator or under light (to prevent the temperature of the chicken embryo from dropping). The placement time should not exceed 20 minutes. Pour out the 0.9% sodium chloride solution. Carefully use forceps to remove the inner membrane, ensuring that the vascular membrane is not damaged. At this time, the structure of the vascular system should be observed again, and its integrity and suitability for the experiment should be judged.

[0043] (2) In the preliminary test, three chicken embryos were used and 0.3 mL of the liquid test substance was taken. The CAM reaction was observed immediately after the test substance was applied for 5 minutes and the observation results were recorded.

[0044] Table 10 Evaluation criteria for flavor solubilizer performance

[0045] Table 11 Solubilizer performance test

[0046] Table 12 Evaluation of stimulation scoring method results

[0047] Table 13 Solubilizer irritation test

[0048] Specifically, the composition is subjected to a mask irritation test, and the process is as follows: S1. Each test sample should be tested using the stimulation scoring method in 10 chicken embryos. Each batch of chicken embryos should have a negative control and a positive control, ≥ 3 each, and a blank solvent should be set up for control testing; S2, take out a chicken embryo of 10 days embryonic age, look after the air chamber with an egg candling device, determine the position of the air chamber, open a small window on the air chamber with a manual drilling tool, peel off the eggshell part of the upper layer of the air chamber with medical ophthalmic curved pointed tweezers to expose the white eggshell membrane, use a 1ml plastic pipette to drip an appropriate amount of 0.9% sodium chloride solution to moisten the eggshell membrane, pour out the 0.9% sodium chloride solution after it is completely moistened, and remove the eggshell membrane with tweezers, and the CAM is exposed after removing the eggshell membrane with rich capillaries (be cautious during the removal process to ensure that the vascular membrane is not damaged); S3. Place a polytetrafluoroethylene resin ring on the CAM as the test area, use a micropipette to transfer 40 µL of the sample to be tested into the polytetrafluoroethylene resin ring, record the time of adding the sample, wet the plastic wrap with sterile water and cover the wet surface of the plastic wrap on the air chamber, move the chicken embryo into a constant temperature and humidity chamber, and set the incubation time to 30 minutes; S4. Take out the tested chicken embryos and score them according to the degree of vascular damage (see Table 1). Use the equipment to take photos of the chorioallantoic membrane before and after the reaction, and then analyze the damage and degree of the CAM by comparing the photos before and after the reaction; S5. The results of each chicken embryo tested should be evaluated and scored. Finally, the highest and lowest scores will be removed from the 10 scores, leaving 8 valid scores, which are then averaged and used to score the irritation.

[0049] Acute eye irritation / corrosion test for adult white rabbits Test method: Gently pull open the lower eyelid of one eye of the rabbit, drop (or apply) 0.1 mL (100 mg) of the test substance into the conjunctival sac, and passively close the upper and lower eyelids for 1 second to prevent the loss of the test substance. The other eye is not treated as a self-control. Do not rinse the eye within 24 hours after dropping the test substance. If necessary, rinse after 24 hours. Before testing, the cosmetic eye irritation-chicken embryo chorioallantoic membrane vascular test should be used for screening to be non-irritating.

[0050] The laboratory further inspects the ingredients of the finished mask, and the table is as follows: Table 14 A non-irritating anti-sugar moisturizing brightening facial mask composition

[0051] As can be seen from Table 14, the composition of the present invention helps to resist skin glycation, thereby protecting the skin from damage caused by glycation and helping to moisturize and brighten the skin.

[0052] The laboratory conducted a preliminary screening of the cosmetic eye irritation - chick embryo chorioallantoic membrane blood vessels on the finished mask of the present invention and ten kinds of masks on the market. The experimental table contains the test score, detection concentration and irritation comparison, and the table is as follows:

[0053] The laboratory conducted an acute eye irritation / corrosion test on adult white rabbits using the finished mask of the present invention and four commercially available masks. The test data is shown in the table below: Table 16 Test data of commercially available facial masks on adult white rabbits for acute eye irritation / corrosion test

[0054] The advantages are: from the above experiments and tables, it can be seen that the mask composition of the present invention has been verified by the chicken embryo chorioallantoic membrane vascular test and the adult white rabbit acute eye irritation / corrosion test, and has the characteristics of non-irritation, can be suitable for consumers with sensitive skin, and will not cause allergic reactions in consumers with sensitive skin. The raw materials of the composition - neohelu acetyl glucosamine and celloco β-glucan can effectively fight skin glycation, protect the skin from glycation damage, and enhance the effect of moisturizing and brightening the skin. Glycerin, 1,2-propylene glycol and cosmetic grade sodium hyaluronate ingredients have deep skin moisturizing effect, can lock moisture for a long time, and solve the problem of dryness. The composition uses skin feel improver (UNILUBE 5OTG-32) PPG-24-glycerol polyether-24 and (BLAUNON GT-100) Fragrance solubilizer acts as a skin feel regulator, moisturizer and fragrance solubilizer, which helps reduce potential irritation or allergic reactions to the skin. The finished composition contains anti-glycation and skin brightening ingredients, which can be used by all skin types and can effectively reduce fine lines, wrinkles and brighten skin tone. Ultimately, it has anti-wrinkle, moisturizing and skin dullness reduction effects for consumers with sensitive skin.

[0055] According to the results of the chicken embryo chorioallantoic membrane vascular test and the adult white rabbit acute eye irritation / corrosion test, the facial mask composition of the present invention was confirmed to be non-irritating, which means that the composition of the present invention is suitable for use on sensitive skin and will not cause discomfort or allergic reactions.

[0056] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A non-irritating anti-sugar moisturizing brightening facial mask composition, characterized in that: The composition includes the following raw materials: water, glycerin, Giga Moist PM-II hydrolyzed sclerotium gum, β-glucan, 1,2-propylene glycol, cosmetic grade sodium hyaluronate, PPG-24-glyceryl polyether-24, neohexyl glucosamine, Kosen K708, fragrance and fragrance solubilizer.

2. The eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition according to claim 1, characterized in that: The weight proportions of the raw materials are: water 92.99%; glycerin 2%; Giga Moist PM-II hydrolyzed sclerotium gum 0.2%; β-glucan 0.03%; 1,2-propylene glycol 3%; cosmetic grade sodium hyaluronate 0.1%; PPG-24-glyceryl polyether-24 1%; Neohexyl acetyl glucosamine 0.1%; Kosen K708 0.6%; flavor 0.05%; flavor solubilizer 0.15%.

3. The eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition according to claim 1, characterized in that: The composition was preliminarily screened for mildness of preservatives using a chicken embryo chorioallantoic membrane vascular test, and no vascular reaction was observed, and the CAM was normal, indicating mildness.

4. The eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition according to claim 1, characterized in that: The composition is subjected to sensory evaluation using a tabletop colorimeter, and the irritation of preservatives is tested. The eye irritation is detected by a test combination. The composition is tested according to the test method of the acute eye irritation / corrosion test for adult white rabbits in the 2015 version of the Cosmetic Safety Technical Specifications: Gently pull open the lower eyelid of one eye of the rabbit, drop (or apply) 0.1 mL (100 mg) of the test substance into the conjunctival sac, and passively close the upper and lower eyelids for 1 second to prevent the loss of the test substance. The other eye is not treated as a self-control. The eye is not rinsed within 24 hours after the test substance is dropped. If necessary, it can be rinsed at 24 hours. Before the test, the cosmetic eye irritation-chicken embryo chorioallantoic membrane vascular test should be used for screening as non-irritating.

5. The eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition according to claim 4, characterized in that: The composition is compared with the appearance of the silver preservative according to the desktop colorimeter test method, and the silver preservative is diluted to 1% with pure water. The test method is to weigh 4-6g of the solution and place it in the detection chamber of the colorimeter, and connect the computer software to determine the specific color.

6. The eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition according to claim 4, characterized in that: The composition is tested for heat stability at 45 degrees Celsius according to the light stability in an ultraviolet weathering test box, wherein the light stability in the ultraviolet weathering test box uses three 340 ultraviolet lamps, and the heat stability at 45 degrees Celsius uses a heat-resistant constant temperature control box as the testing equipment.

7. The eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition according to claim 1, characterized in that: The composition was tested for solubilizing performance and irritation performance.

8. The eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition according to claim 7, characterized in that: The solubilizer performance test is performed as follows: add 0.05% flavor, 0.15% solubilizer, and 99.8% pure water, mix and stir evenly, and compare the transparency of the aqueous solution.

9. The eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition according to claim 7, characterized in that: The solubilizer irritation test is conducted according to the irritation scoring method in the chicken chorioallantoic membrane test method for eye irritation / corrosion of cosmetics in the 2009 Entry-Exit Inspection and Quarantine Industry Standard of the People's Republic of China. When the test score is IS<1, it is evaluated as non-irritating, and the BLAUNON GT-100 fragrance solubilizer in the composition is evaluated as non-irritating.

10. The eye-friendly, non-irritating, anti-sugar, moisturizing and brightening facial mask composition according to claim 1, characterized in that: The composition is subjected to a mask irritation test, and the process is as follows: S1. Each test sample should be tested using the stimulation scoring method in 10 chicken embryos. Each batch of chicken embryos should have a negative control and a positive control, ≥ 3 each, and a blank solvent should be set up for control testing; S2, take out a chicken embryo of 10 days embryonic age, look after the air chamber with an egg candling device, determine the position of the air chamber, open a small window on the air chamber with a manual drilling tool with a hand-twisted drill, peel off the eggshell part on the upper strata of the air chamber with medical ophthalmic curved pointed tweezers, expose the white eggshell membrane, use a 1ml plastic pipette to drip an appropriate amount of 0.9% sodium chloride solution to moisten the eggshell membrane, pour out the 0.9% sodium chloride solution after completely moistening, and remove the eggshell membrane with tweezers, and the CAM is exposed after removing the eggshell membrane with abundant capillaries; S3. Place a polytetrafluoroethylene resin ring on the CAM as the test area, use a micropipette to transfer 40 µL of the sample to be tested into the polytetrafluoroethylene resin ring, record the time of adding the sample, wet the plastic wrap with sterile water and cover the wet surface of the plastic wrap on the air chamber, move the chicken embryo into a constant temperature and humidity chamber, and set the incubation time to 30 minutes; S4. Take out the tested chicken embryos, score the degree of vascular damage, use high-definition camera equipment to take photos and record the chorioallantoic membrane before and after the reaction, and analyze the damage and degree of CAM by comparing the photos before and after the reaction; S5. The results of each chicken embryo tested should be evaluated and scored. Finally, the highest and lowest scores will be removed from the 10 scores, leaving 8 valid scores, which are then averaged and used to score the irritation.

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