Application of traditional Chinese medicine composition in preparation of medicine for improving or treating steroid metabolic disorder

Abstract

The invention relates to an application of a traditional Chinese medicine composition in preparation of a medicine for improving or treating steroid metabolic disorder. The traditional Chinese medicine composition comprises astragalus membranaceus, bighead atractylodes rhizome, Chinese yam, cistanche, amomum cardamomum, salvia miltiorrhiza, raw rhubarb and honey-fried licorice root. The traditional Chinese medicine composition provided by the invention is prepared by compounding a plurality of traditional Chinese medicine components which are matched with one another and have a synergistic effect, so that the technical effect of effectively improving or treating steroid metabolic disorder can be achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to application of a traditional Chinese medicine composition in preparing a medicine for improving or treating steroid metabolic disorders. Background Art

[0002] The endocrine system, nervous system and immune system in the human body jointly bear the important responsibility of sustaining life, and hormones are the material basis for the coordination of the endocrine system. Steroid hormones, also known as steroid hormones, belong to a large category of hormones. Their synthesis process uses cholesterol as a precursor. Through the participation of various metabolic enzymes, they are synthesized in the human adrenal cortex, mitochondria of placental cells, smooth endoplasmic reticulum and gonads to produce 21C, 19C, and 18C skeletons with different chemical structures.

[0003] According to the receptors they bind to, steroid hormones can be divided into two categories: sex hormones (including estrogen, androgen and progesterone) and corticosteroids (including glucocorticoids and mineralocorticoids). According to their sources, steroid hormones can be divided into endogenous and exogenous steroid hormones. Endogenous steroid hormones are naturally synthesized by the body's secretory organs or tissues to maintain homeostasis; exogenous steroid hormones are artificially synthesized or excreted by other organisms and are endocrine disrupting compounds (EDCs).

[0004] Steroid hormones play an important regulatory role in human growth, development, reproduction, and maintaining the stability of the internal environment. Hormones work by binding to receptors on human target cells and have extremely high specificity. When the level of steroid hormones in the body is disordered, the body will produce severe syndromes and even endanger life. Therefore, clinically, steroid hormone levels are used as diagnostic indicators for many diseases, such as congenital adrenal hyperplasia, Cushing's syndrome, primary aldosteronism, and some mental illnesses.

[0005] Therefore, providing a drug with good efficacy, high safety and the ability to specifically treat steroid metabolic disorders is a technical problem that needs to be urgently solved in the field. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides an application of a Chinese medicine composition in the preparation of a drug for improving or treating steroid metabolic disorders. The Chinese medicine composition provided by the present invention is compounded with a plurality of Chinese medicine ingredients, and each ingredient cooperates with each other and synergistically enhances the effect, thereby achieving the technical effect of effectively improving or treating steroid metabolic disorders.

[0007] To achieve this object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a use of a traditional Chinese medicine composition in the preparation of a medicament for improving or treating steroid metabolism disorders, wherein the traditional Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice.

[0009] The clinical manifestations of steroid metabolism disorder belong to the categories of "water dampness" and "fat turbidity" in traditional Chinese medicine, which are caused by spleen and kidney deficiency and dampness turbidity retention in the body. In the formula, Astragalus, Atractylodes macrocephala, and Chinese yam nourish the spleen and stomach, and Cistanche deserticola nourishes the kidneys. Salvia miltiorrhiza nourishes blood and activates blood circulation, nourishes both the spleen and kidneys, replenishes qi and blood, and transports and removes turbidity; white cardamom removes dampness and promotes qi, rhubarb removes turbidity downward, and roasted licorice harmonizes the various medicines. The whole formula takes into account both tonification and purgation, tonifies the spleen and kidneys and eliminates dampness and turbidity, thereby regulating steroid metabolism.

[0010] The Chinese medicine composition provided by the present invention is prepared by compounding a plurality of Chinese medicine ingredients, and the various ingredients cooperate with each other and synergistically enhance the effect, thereby achieving the technical effect of effectively improving or treating steroid metabolic disorders.

[0011] Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice. In the present invention, the portion of Astragalus can be 11.2 parts, 11.5 parts, 11.8 parts, 12 parts, 12.2 parts, 12.5 parts, 12.8 parts, etc.; the portion of Atractylodes can be 7.2 parts, 7.5 parts, 7.8 parts, 8 parts, 8.2 parts, 8.5 parts, 8.8 parts, etc.; the portion of Chinese yam can be 11.2 parts, 11.5 parts, 11.8 parts, 12 parts, 12.2 parts, 12.5 parts, 12.8 parts, etc.; the portion of Cistanche can be 3.2 parts, 3.5 parts, 3.8 parts, 4 parts, 4.2 parts, 4.5 parts, 4 .8 parts, etc.; the number of white cardamom can be 3.2 parts, 3.5 parts, 3.8 parts, 4 parts, 4.2 parts, 4.5 parts, 4.8 parts, etc.; the number of salvia miltiorrhiza can be 11.2 parts, 11.5 parts, 11.8 parts, 12 parts, 12.2 parts, 12.5 parts, 12.8 parts, etc.; the number of raw rhubarb can be 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, etc.; the number of roasted licorice can be 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, etc.

[0012] When the Chinese medicine composition is formulated in the above-mentioned specific mass fraction, it has a better technical effect in regulating steroid metabolic disorders.

[0013] Preferably, the drug promotes an increase in 7-enecholestanol.

[0014] Preferably, the drug promotes an increase in beta-sitosterol.

[0015] Preferably, the drug promotes an increase in 7-dehydrocholesterol.

[0016] Preferably, the drug promotes elevation of lanosterol.

[0017] Preferably, the drug promotes an increase in 25-hydroxycholesterol.

[0018] Preferably, the medicament inhibits the rise of fecal alcohol.

[0019] Preferably, the drug further comprises a pharmaceutically acceptable excipient.

[0020] Preferably, the preparation method of the traditional Chinese medicine composition comprises the following steps: crushing Atractylodes macrocephala, Dioscorea opposita, White Cardamom, Astragalus, Cistanche deserticola, Salvia miltiorrhiza, Rhubarb and Radix Glycyrrhizae into fine powder, mixing them evenly, and obtaining the traditional Chinese medicine composition.

[0021] Preferably, the auxiliary material comprises any one of an excipient, a diluent, a carrier, a flavoring agent, a binder or a filler, or a combination of at least two thereof.

[0022] Preferably, the carrier comprises a liposome, a micelle, a microsphere or a microcapsule.

[0023] Preferably, the dosage form of the drug includes tablets, capsules, granules, powders, injections, sprays or drops.

[0024] In a second aspect, the present invention provides an application of a traditional Chinese medicine composition in a 7-enecholestanol promoter, wherein the traditional Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice.

[0025] Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

[0026] In a third aspect, the present invention provides an application of a traditional Chinese medicine composition in a β-sitosterol promoter, wherein the traditional Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice.

[0027] Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

[0028] In a fourth aspect, the present invention provides an application of a traditional Chinese medicine composition in a 7-dehydrocholesterol promoter, wherein the traditional Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice.

[0029] Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

[0030] In a fifth aspect, the present invention provides an application of a traditional Chinese medicine composition in a lanosterol promoter, wherein the traditional Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice.

[0031] Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

[0032] In a sixth aspect, the present invention provides an application of a traditional Chinese medicine composition in a 25-hydroxycholesterol promoter, characterized in that the traditional Chinese medicine composition includes astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted licorice.

[0033] Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

[0034] In a seventh aspect, the present invention provides an application of a traditional Chinese medicine composition in a fecal alcohol inhibitor, wherein the traditional Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice.

[0035] Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

[0036] Compared with the prior art, the present invention has at least the following beneficial effects:

[0037] The Chinese medicine composition provided by the invention comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice, wherein the components cooperate with each other and synergistically enhance the effect, and can effectively improve or treat steroid metabolic disorders. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is a graph showing the effect of the Chinese medicine composition provided in Example 1 on the content of 7-enecholestanol.

[0039] Figure 2 This is a graph showing the effect of the Chinese medicine composition provided in Example 1 on the content of β-sitosterol.

[0040] Figure 3 This is a graph showing the effect of the Chinese medicine composition provided in Example 1 on the content of 7-dehydrocholesterol.

[0041] Figure 4 This is a graph showing the effect of the Chinese medicine composition provided in Example 1 on the lanosterol content.

[0042] Figure 5 This is a graph showing the effect of the Chinese medicine composition provided in Example 1 on the content of 25-hydroxycholesterol.

[0043] Figure 6 This is a graph showing the effect of the Chinese medicine composition provided in Example 1 on the fecal alcohol content. DETAILED DESCRIPTION

[0044] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and through specific implementation methods. However, the following examples are only simplified examples of the present invention and do not represent or limit the scope of protection of the present invention. The scope of protection of the present invention shall be subject to the claims.

[0045] The astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted licorice used in the following examples were provided by the pharmacy of Shenzhen Traditional Chinese Medicine Hospital.

[0046] Example 1

[0047] The present embodiment provides an application of a traditional Chinese medicine composition in the preparation of a drug for improving or treating steroid metabolism disorders, wherein the traditional Chinese medicine composition comprises, by weight, 12 parts of astragalus, 8 parts of atractylodes, 12 parts of yam, 4 parts of cistanche, 4 parts of white cardamom, 12 parts of salvia miltiorrhiza, 2 parts of raw rhubarb and 2 parts of roasted liquorice.

[0048] The preparation method of the traditional Chinese medicine composition comprises the following steps: crushing the above raw materials into fine powder, mixing them evenly, and obtaining the traditional Chinese medicine composition.

[0049] Example 2

[0050] The present embodiment provides an application of a traditional Chinese medicine composition in the preparation of a drug for improving or treating steroid metabolism disorders, wherein the traditional Chinese medicine composition comprises, by weight, 11 parts of astragalus, 8 parts of atractylodes, 12 parts of yam, 4 parts of cistanche, 4 parts of white cardamom, 12 parts of salvia miltiorrhiza, 2 parts of raw rhubarb and 2 parts of roasted liquorice.

[0051] The preparation method of the Chinese medicine composition is as shown in Example 1.

[0052] Example 3

[0053] The present embodiment provides an application of a traditional Chinese medicine composition in the preparation of a drug for improving or treating steroid metabolism disorders, wherein the traditional Chinese medicine composition comprises, by weight, 13 parts of astragalus, 8 parts of atractylodes, 12 parts of yam, 4 parts of cistanche, 4 parts of white cardamom, 12 parts of salvia miltiorrhiza, 2 parts of raw rhubarb and 2 parts of roasted liquorice.

[0054] The preparation method of the Chinese medicine composition is as shown in Example 1.

[0055] Comparative Example 1

[0056] This comparative example provides an application of a traditional Chinese medicine composition in the preparation of a medicament for improving or treating steroid metabolism disorders. The traditional Chinese medicine composition comprises, by mass, 8 parts of Atractylodes macrocephala, 12 parts of Dioscorea opposita, 4 parts of Cistanche deserticola, 4 parts of White Cardamom, 12 parts of Salvia miltiorrhiza, 2 parts of Raw Rhubarb and 2 parts of Radix Glycyrrhizae.

[0057] The preparation method of the Chinese medicine composition is as shown in Example 1.

[0058] Test Example 1

[0059] Test sample: the Chinese medicine composition provided in Example 1.

[0060] Test method:

[0061] (1) Animal rearing conditions: Male Spraque-Dawley (SD) rats weighing 180-220 g were purchased from Vital River Company. The room temperature was controlled at 21°C ± 2°C, the relative humidity was controlled at 50% ± 15%, and the light cycle was 12 hours. The SD rats were adaptively reared for 1 week and were allowed to eat and drink freely.

[0062] (2) Experimental drugs: 1% Chinese medicine composition feed was obtained by mixing the Chinese medicine composition provided in Example 1 and rat maintenance feed at a mass ratio of 1:99; 0.75% adenine feed was obtained by mixing adenine granules and rat maintenance feed at a mass ratio of 0.75:99.25.

[0063] (3) Establishment and grouping of animal models: After one week of adaptive feeding, the rats were divided into three groups, with 8 rats in each group, namely: normal group, model group and Chinese medicine group; the rats in the normal group were fed with rat maintenance feed normally, and the rats in the model group and Chinese medicine group were fed with 0.75% adenine feed for 3 weeks to cause steroid metabolism disorders in the rats; after successful modeling, the Chinese medicine group was fed with 1% Chinese medicine composition feed every day, and the normal group and model group were fed with the same amount of rat maintenance feed. Samples were collected after 4 weeks of feeding.

[0064] (4) Sample collection: After anesthesia, the rats were fixed on the operating table. A midline incision was made in the abdomen to open the abdominal cavity. Blood was collected from the abdominal aorta. After standing at room temperature for 2 hours, the blood was centrifuged at 3000 rpm for 10 min at 4°C to collect serum.

[0065] (5) Index determination: The steroid content (7-enecholestanol, β-sitosterol, 7-dehydrocholesterol, lanosterol, and fecal alcohol) in rat serum was determined.

[0066] The steroid content in the rat serum was determined, and the results are shown in Table 1. Figure 1-6 shown.

[0067] Table 1

[0068]

[0069] Test Example 2

[0070] Test sample: the Chinese medicine composition provided in Comparative Example 1.

[0071] The Chinese medicine composition provided in Example 1 in Test Example 1 was replaced with the Chinese medicine composition provided in Comparative Example 1 (Chinese medicine group without Astragalus). The testing method was the same as that in Test Example 1. The results are shown in Table 2.

[0072] Table 2

[0073]

[0074] Since Test Example 1 and Test Example 2 were carried out in different batches, the data had a batch effect. In order to more accurately represent the changes after the intervention, the present invention compared the data of the traditional Chinese medicine group or the traditional Chinese medicine group without astragalus with the corresponding model group to obtain the percentage change of the average content of the two compared with the model group. The results showed that the traditional Chinese medicine composition provided in Example 1 can more significantly promote the increase of 7-enecholestanol, β-sitosterol, 7-dehydrocholesterol, and lanosterol, inhibit the increase of fecal alcohol, and achieve the technical effect of effectively regulating steroid metabolic disorders; further, when the traditional Chinese medicine group without astragalus provided in comparative example 1 was used, the content of fecal alcohol did not decrease but increased, thereby proving the compounding effect between the components of the traditional Chinese medicine composition provided by the present invention.

[0075] In summary, the present invention can promote the increase of 7-enecholestanol, β-sitosterol, 7-dehydrocholesterol, lanosterol, and 25-hydroxycholesterol, inhibit the increase of fecal alcohol, and achieve the technical effect of effectively regulating steroid metabolic disorders through reasonable compounding of eight traditional Chinese medicine ingredients.

[0076] The applicant declares that the above is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention shall fall within the protection scope and disclosure scope of the present invention.

Claims

1. Use of a Chinese medicine composition in the preparation of a drug for improving or treating steroid metabolic disorders, characterized in that: The traditional Chinese medicine composition comprises astragalus, atractylodes macrocephala, yam, cistanche deserticola, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice.

2. The use according to claim 1, characterized in that: The Chinese medicine composition comprises, by weight, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

3. The use according to claim 1 or 2, characterized in that: The drug promotes the increase of 7-enecholestanol; Preferably, the drug promotes an increase in β-sitosterol; Preferably, the drug promotes an increase in 7-dehydrocholesterol; Preferably, the drug promotes an increase in lanosterol; Preferably, the drug promotes an increase in 25-hydroxycholesterol; Preferably, the medicament inhibits the rise of fecal alcohol.

4. The use according to any one of claims 1 to 3, characterized in that: The preparation method of the traditional Chinese medicine composition comprises the following steps: crushing Atractylodes macrocephala, Chinese yam, white cardamom, astragalus, Cistanche deserticola, Salvia miltiorrhiza, raw rhubarb and roasted liquorice into fine powder, and mixing them evenly to obtain the traditional Chinese medicine composition; Preferably, the drug further comprises a pharmaceutically acceptable excipient; Preferably, the auxiliary material comprises any one or a combination of at least two of an excipient, a diluent, a carrier, a flavoring agent, a binder or a filler; Preferably, the carrier comprises a liposome, a micelle, a microsphere or a microcapsule; Preferably, the dosage form of the drug includes tablets, capsules, granules, powders, injections, sprays or drops.

5. Use of a Chinese medicine composition in a 7-enecholestanol promoter, characterized in that: The Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice; Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

6. Use of a Chinese medicine composition in a β-sitosterol promoter, characterized in that: The Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice; Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

7. Use of a Chinese medicine composition in a 7-dehydrocholesterol promoter, characterized in that: The Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice; Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

8. Use of a Chinese medicine composition in a lanosterol promoter, characterized in that: The Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice; Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

9. Use of a Chinese medicine composition in a 25-hydroxycholesterol promoter, characterized in that: The Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice; Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

10. Use of a Chinese medicine composition in a fecal alcohol inhibitor, characterized in that: The Chinese medicine composition comprises astragalus, atractylodes, yam, cistanche, white cardamom, salvia miltiorrhiza, raw rhubarb and roasted liquorice; Preferably, the Chinese medicine composition comprises, by mass, 11-13 parts of astragalus, 7-9 parts of atractylodes, 11-13 parts of yam, 3-5 parts of cistanche, 3-5 parts of white cardamom, 11-13 parts of salvia miltiorrhiza, 1-3 parts of raw rhubarb and 1-3 parts of roasted liquorice.

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