Preparation method of plant exosome eutrophic liquid

Abstract

The invention belongs to the technical field of eutrophication liquid processing, and provides a preparation method of a plant exosome eutrophication liquid, which comprises the following steps: S1, collecting fresh plant materials including but not limited to leaves, fruits and flowers, and cleaning the fresh plant materials; s2, cutting the cleaned plant material into small blocks, putting the small blocks into a centrifugal machine, and centrifuging for a period of time at a proper rotating speed; s3, the centrifuged supernate is filtered through a filter, and a pure plant exosome solution is obtained; s4, collecting the filtered plant exosome solution into a sterile container; through fine centrifugal separation, multi-stage filtration, a customized formula and optimized storage conditions, the preparation efficiency, the component purity and the biological activity maintaining capacity of the plant exosome eutrophic liquid are remarkably improved, meanwhile, the effect of the plant exosome eutrophic liquid in practical application is enhanced, for example, plant growth is promoted, the yield is increased, environmental protection and sustainability are emphasized, and the plant exosome eutrophic liquid is suitable for industrial production. A more efficient, reliable and environment-friendly method is provided for preparation and application of the plant exosome eutrophic liquid.

Description

Technical Field

[0001] The invention belongs to the technical field of nutrient-rich solution processing, and specifically relates to a method for preparing a plant exosome nutrient-rich solution. Background Art

[0002] Plant exosome-enriched nutrient solution is a nutrient solution containing plant exosomes, which has multiple biological activities and nutrients. However, traditional preparation methods often have problems such as low efficiency and impure ingredients, which limits its effectiveness in practical applications.

[0003] To this end, those skilled in the art have proposed a method for preparing a plant exosome-rich nutrient solution to solve the problems raised by the background technology. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a method for preparing a plant exosome-enriched nutrient solution to solve the problems that the traditional preparation methods in the prior art often have low efficiency and impure ingredients, which limit their effects in practical applications.

[0005] A method for preparing a plant exosome-enriched nutrient solution, comprising:

[0006] S1. Collect fresh plant materials, including but not limited to leaves, fruits, and flowers, and clean them;

[0007] S2. Cut the cleaned plant material into small pieces, put them into a centrifuge, and centrifuge them at an appropriate speed for a period of time to separate the supernatant containing plant exosomes. The centrifugation speed and time are optimized and adjusted according to the type and properties of the plant material;

[0008] S3, filtering the supernatant after centrifugation through a filter to remove impurities and cell debris to obtain a pure plant exosome solution; the filter has an appropriate pore size to effectively remove impurities and cell debris;

[0009] S4. Collect the filtered plant exosome solution into a sterile container, and add an appropriate amount of culture medium or nutrient solution to adjust the concentration of nutrients to obtain a plant exosome-enriched nutrient solution; the type and concentration of the added culture medium or nutrient solution are optimized and adjusted according to the intended use of the plant exosome-enriched nutrient solution;

[0010] S5, then further processing the plant exosome-rich nutrient solution, including adding other nutrients and adjusting the pH value;

[0011] S6. Store the prepared plant exosome-rich solution under appropriate conditions, including low temperature and protection from light, to maintain its stability and biological activity.

[0012] Preferably, the step S1 is specifically described as follows:

[0013] S1.1. Select plants that are vigorously growing and free of pests and diseases as raw materials, and give priority to plant varieties or parts with efficient exosome secretion capabilities, including specific leaves rich in bioactive ingredients, mature and plump fruits, and flowers with bright colors and strong fragrance. During the collection process, pay attention to avoid mechanical damage to the plants to maintain the integrity of the plant materials;

[0014] S1.2. Immediately clean the collected plant materials to remove dust, soil and other impurities on the surface; when cleaning, use clean water at an appropriate temperature and avoid using detergents containing chemical ingredients to avoid contaminating the plant materials or destroying their biologically active ingredients;

[0015] S1.3. Classify the plant materials after preliminary cleaning and subdivide them according to the type, growth part and intended use of the plant materials; for different types of plant materials, use targeted cleaning methods and tools to ensure the best cleaning effect;

[0016] S1.4. After classification and cleaning, further disinfect the plant materials; use ultraviolet disinfection, ozone disinfection or chemical disinfectants to effectively kill bacteria, viruses and other microorganisms on the surface of the plant materials to avoid the introduction of pollutants in the subsequent preparation process;

[0017] S1.5. Dry the disinfected plant materials to remove excess moisture. When drying, pay attention to maintaining appropriate temperature and humidity conditions to prevent the plant materials from losing their biologically active components due to excessive drying or from causing mildew due to excessive humidity.

[0018] S1.6. Finally, the quality of the dried plant materials is checked to ensure that the surface of the plant materials is clean, undamaged, and uncontaminated, and that they meet the requirements for preparing plant exosome-enriched nutrient solution; plant materials that do not meet the requirements are discarded or reprocessed.

[0019] Through the above steps, not only the cleanliness of the plant materials and the integrity of the bioactive components are ensured, but also the preparation efficiency and quality of the plant exosome-rich nutrient solution are improved.

[0020] Preferably, the step S2 is specifically described as follows:

[0021] S2.1. Use sharp knives or mechanical cutting equipment to finely cut the cleaned and pretreated plant materials into small pieces or slices. When cutting, pay attention to maintaining the consistency of the cutting size to ensure the effect of subsequent centrifugal separation. At the same time, avoid excessive damage to plant cells to reduce the generation of cell debris and impurities.

[0022] S2.2. Place the cut plant materials evenly into the centrifuge tube of the centrifuge to ensure that the plant materials in the centrifuge tube are evenly distributed to avoid local overload or imbalance during the centrifugation process;

[0023] S2.3. Select appropriate centrifugation speed and time according to the type of plant material, growth site and characteristics of bioactive components; for plant materials rich in exosomes, use a higher centrifugation speed and appropriate centrifugation time to fully separate the supernatant containing plant exosomes; at the same time, pay attention to monitoring the temperature changes during the centrifugation process to avoid high temperature from damaging the biological activity of plant exosomes;

[0024] S2.4. During the centrifugal separation process, dynamic monitoring technology is used to observe the stratification of the liquid in the centrifuge tube and the clarity of the supernatant in real time; when the supernatant becomes clear and the stratification is obvious, the centrifugal operation is stopped;

[0025] S2.5. Carefully remove the supernatant after centrifugation to avoid contact with the sediment at the bottom of the centrifuge tube; at the same time, perform a quality check on the removed supernatant to ensure that its clarity, purity and content of bioactive components meet the requirements for preparing plant exosome-enriched nutrient solution;

[0026] S2.6. For plant materials of different types and properties, record the optimal centrifugation speed and time parameters for reference and optimization in subsequent preparation processes.

[0027] Through the above steps, not only the separation efficiency and purity of plant exosomes are improved, but also the stability and controllability of the preparation process are ensured.

[0028] Preferably, the step S3 is specifically described as follows:

[0029] S3.1. The supernatant obtained after centrifugation is first filtered through a coarse filter to remove larger impurities and cell debris; the pore size of the coarse filter is selected according to the particle size and distribution of impurities in the supernatant to ensure that most impurities can be effectively removed;

[0030] S3.2. The supernatant after the coarse filtration is further filtered through the medium filter to remove smaller impurities and cell debris; the pore size of the medium filter is finer than that of the coarse filter, which can ensure that the purity and transparency of the supernatant are further improved;

[0031] S3.3. In order to obtain a plant exosome solution with higher purity, the supernatant after the intermediate filtration is finally filtered through a precision filter; the pore size of the precision filter is selected according to the size and shape of the plant exosomes to ensure that tiny impurities and cell fragments can be effectively removed while retaining intact plant exosomes;

[0032] S3.4. During the filtration process, pressure control technology is used to ensure that the filtration pressure is moderate to avoid mechanical damage to plant exosomes or destruction of their biological activity. At the same time, filter blockage and filtration efficiency are monitored regularly, and clogged filters are replaced or filtration parameters are adjusted in a timely manner.

[0033] S3.5. Perform quality inspection on the plant exosome solution after multi-stage filtration, including indicators such as purity, transparency and content of bioactive components; ensure that the plant exosome solution meets the requirements for preparing plant exosome-enriched nutrient solution;

[0034] S3.6. In order to further improve the purity and stability of the plant exosome solution, membrane separation technologies such as ultrafiltration, nanofiltration or reverse osmosis can be used for further purification. Appropriate membrane separation technology and operating parameters can be selected according to the size and properties of the plant exosomes.

[0035] Through the above steps, multi-stage filtration and purification of the plant exosome solution was achieved, impurities and cell debris were effectively removed, the purity and stability of the plant exosome solution were improved, and high-quality raw materials were provided for the subsequent preparation of plant exosome-enriched nutrient solution.

[0036] Preferably, the step S4 is specifically described as follows:

[0037] S4.1. Collect the purified plant exosome solution obtained after multi-stage filtration into a sterile container that has been sterilized to ensure a sterile environment to avoid contamination by bacteria and other contaminants;

[0038] S4.2. According to the intended use of the plant exosome-enriched nutrient solution and the growth requirements of the target plants, a customized selection of suitable culture medium or nutrient solution is made; this includes but is not limited to inorganic salts, organic nutrients, vitamins, trace elements, plant growth regulators and other ingredients;

[0039] S4.3. After determining the type of culture medium or nutrient solution, accurately calculate and adjust the concentration of each nutrient component according to the growth stage, growth rate and bioactive component requirements of the target plant; by adjusting the concentration of the nutrient components, the nutrient supply of the plant exosome-enriched nutrient solution can be optimized to promote the growth and development of the target plant;

[0040] S4.4. When adding culture medium or nutrient solution, gradually add and mix thoroughly to ensure uniform distribution of nutrients and avoid excessive or low local concentrations. At the same time, regularly monitor the pH value and osmotic pressure of the plant exosome-enriched nutrient solution to ensure that it is within an appropriate range.

[0041] S4.5. Conduct quality inspection on the prepared plant exosome-enriched nutrient solution, including indicators such as the content, uniformity and stability of nutrients; ensure that the plant exosome-enriched nutrient solution meets the expected requirements and has a good application effect;

[0042] S4.6. In order to further improve the adaptability and stability of the plant exosome-enriched nutrient solution, stability tests and adaptability assessments can be performed on it. Based on the test results and assessment results, the formula and nutritional components of the plant exosome-enriched nutrient solution can be optimized and adjusted to meet the needs of different plants and under different growth conditions.

[0043] Through the above steps, customized formulation and nutritional adjustment of plant exosome-enriched nutrient solution are achieved, its adaptability and stability are improved, and high-quality plant exosome-enriched nutrient solution is provided for applications on different plants and under different growth conditions.

[0044] Preferably, the step S5 is specifically described as follows:

[0045] S5.1. Conduct a comprehensive performance evaluation of the prepared plant exosome-enriched nutrient solution, including but not limited to indicators such as nutrient content, pH value, osmotic pressure, stability and biological activity; ensure that it can meet the growth needs and expected effects of the target plants in subsequent applications;

[0046] S5.2. Determine the types and quantities of other nutrients that need to be added based on the performance evaluation results and the growth requirements of the target plants; this includes amino acids, nucleotides, plant hormones, antioxidants or other bioactive substances, etc., to further improve the biological activity and nutritional value of the plant exosome-enriched nutrient solution;

[0047] S5.3. When adding other nutrients, use precise measurement and gradual addition to ensure that each ingredient can be evenly distributed in the plant exosome-enriched nutrient solution to avoid local concentrations that are too high or too low. At the same time, regularly monitor the composition changes and stability of the plant exosome-enriched nutrient solution and adjust the addition strategy in a timely manner.

[0048] S5.4. Accurately adjust the pH value of the plant exosome-rich nutrient solution to ensure that it is within the appropriate growth range; use a precision pH meter for monitoring and adjustment, and select appropriate acid-base regulators for adjustment based on the composition of the plant exosome-rich nutrient solution and the growth requirements of the target plants; at the same time, pay attention to avoid introducing new pollutants or destroying the biological activity of plant exosomes during the adjustment process;

[0049] S5.5. After adjusting the pH value, conduct another quality inspection and stability test on the plant exosome-enriched nutrient solution to ensure that it can maintain stable performance in subsequent applications and meet the growth requirements of the target plants;

[0050] S5.6. In order to further improve the adaptability and application range of plant exosome-enriched nutrient solution, its formula can be optimized and its performance improved; according to the feedback and test results in actual applications, the composition, concentration and preparation process of plant exosome-enriched nutrient solution can be adjusted to meet the needs of different plants and under different growth conditions.

[0051] Through the above steps, the fine regulation and performance enhancement of the plant exosome-enriched nutrient solution are achieved, its biological activity, nutritional value and adaptability are improved, and a better quality plant exosome-enriched nutrient solution is provided for application on different plants and under different growth conditions.

[0052] Preferably, the step S6 is specifically described as follows:

[0053] S6.1. Conduct a preliminary quality assessment on the prepared plant exosome-enriched nutrient solution to confirm that it meets the expected biological activity, nutritional value and stability requirements; only products that have passed strict quality control can be subsequently stored;

[0054] S6.2. Select appropriate storage containers to ensure that the plant exosome-rich nutrient solution will not be contaminated or lost during storage. The storage container should have good sealing, low temperature resistance and light-proof properties, and be able to withstand certain pressure changes to prevent damage from external factors.

[0055] S6.3. Set a suitable storage temperature to ensure that the plant exosome-rich solution is stored in a low temperature environment to slow down its degradation rate and prolong the storage time; the specific storage temperature should be determined based on the composition, concentration and stability test results of the plant exosome-rich solution, and it should be stored below 4°C;

[0056] S6.4. In addition to low-temperature storage, it is also necessary to pay attention to light-proof storage to prevent light from damaging the bioactive components in the plant exosome-rich nutrient solution. The storage container should be placed in an area where light cannot directly reach it, or be shielded with light-proof materials.

[0057] S6.5. Regularly check the quality of stored plant exosome-enriched nutrient solution, including testing its biological activity, nutritional value, stability and other indicators; if the quality is found to be degraded or not meeting the requirements, stop using it immediately and dispose of it;

[0058] S6.6. In order to further improve the storage stability of plant exosome-enriched nutrient solution, some advanced storage technologies can be used, such as vacuum packaging, freeze drying or modified atmosphere packaging; these technologies can effectively extend the shelf life of plant exosome-enriched nutrient solution and maintain its original quality and biological activity;

[0059] S6.7. Establish a complete storage record and tracking system to keep track of the storage status and usage history of the plant exosome-enriched nutrient solution; this will help to promptly identify and resolve problems and ensure the safety and effectiveness of the plant exosome-enriched nutrient solution.

[0060] Through the above steps, the storage conditions of the plant exosome-rich nutrient solution are optimized and the quality requirements are maintained for a long time, the stability and biological activity of the plant exosome-rich nutrient solution are improved, and a more reliable guarantee is provided for practical applications.

[0061] Preferably, the preparation process of the plant exosome-rich nutrient solution is carried out under sterile conditions to avoid contamination by bacteria and other pollutants.

[0062] Materials and equipment required for the above method: Materials and equipment:

[0063] -Fresh plant materials (such as leaves, fruits, flowers, etc.);

[0064] - centrifuge;

[0065] - Filters;

[0066] - Sterile containers;

[0067] - Culture medium or nutrient solution.

[0068] Compared with the prior art, the present invention has the following beneficial effects:

[0069] The present invention significantly improves the preparation efficiency, component purity and biological activity retention ability of plant exosome-enriched nutrient solution through fine centrifugal separation, multi-stage filtration, customized formulation and optimized storage conditions, while enhancing its effects in practical applications, such as promoting plant growth and increasing yield, and pays attention to environmental protection and sustainability, providing a more efficient, reliable and environmentally friendly method for the preparation and application of plant exosome-enriched nutrient solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0070] Figure 1 The present invention is a flow chart of the method for preparing the plant exosome-enriched nutrient solution. DETAILED DESCRIPTION

[0071] The following embodiments of the present invention are described in further detail in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0072] Embodiment: The present invention provides a method for preparing a plant exosome-enriched nutrient solution, such as Figure 1 As shown, including:

[0073] S1. Collect fresh plant materials, including but not limited to leaves, fruits, and flowers, and clean them;

[0074] S2, cutting the cleaned plant material into small pieces, placing them in a centrifuge, and centrifuging them at an appropriate speed for a period of time to separate the supernatant containing plant exosomes;

[0075] S3, filtering the supernatant after centrifugation through a filter to remove impurities and cell debris to obtain a pure plant exosome solution;

[0076] S4, collecting the filtered plant exosome solution into a sterile container, and adding an appropriate amount of culture medium and nutrient solution to adjust the concentration of nutrients to obtain a plant exosome-rich nutrient solution;

[0077] S5, then further processing the plant exosome-rich nutrient solution;

[0078] S6. Store the prepared plant exosome-rich solution under appropriate conditions to maintain its stability and biological activity.

[0079] From the above, it can be seen that the preparation method of the plant exosome-enriched nutrient solution has the following beneficial effects:

[0080] 1) The present invention can effectively separate the supernatant containing plant exosomes and remove impurities and cell debris through fine centrifugation and multi-stage filtration steps (S2 and S3), thereby obtaining a high-purity plant exosome solution; this method can better ensure the integrity and biological activity of exosomes than traditional methods;

[0081] 2) In the preparation process (S4) of the present invention, the type and concentration of the culture medium and nutrient solution are customized and adjusted according to the intended use of the plant exosome-enriched nutrient solution and the growth requirements of the target plants; the customized formula can meet the needs of different plants at different growth stages and improve the pertinence and effectiveness of nutrient supply;

[0082] 3) The present invention can enhance the biological activity and stability of the plant exosome-rich nutrient solution by further processing it (S5), such as adding other nutrients, adjusting the pH value, etc. These processing steps help to maintain the balance and stability of various nutrients in the nutrient solution, thereby improving its effect in practical applications;

[0083] 4) In the storage stage (S6), the present invention can effectively extend the shelf life of the plant exosome-enriched nutrient solution by selecting a suitable storage container, setting a suitable storage temperature and light-proof conditions; these measures help maintain the stability and biological activity of the nutrient solution, ensuring that it always remains in the best state during use;

[0084] 5) The preparation method provided by the present invention has clear steps and standardized operation, and is easy to implement controllable and repeatable operation in the laboratory or industrial production; this helps to ensure that the plant exosome-enriched nutrient solution prepared each time has consistent quality and effect;

[0085] 6) Since the plant exosome-enriched nutrient solution contains high-purity plant exosomes and customized nutrients, it can promote the growth and development of plants; in practical applications, it helps to improve the yield and quality of crops, or promote the growth and ornamental value of ornamental plants;

[0086] 7) The present invention pays attention to environmental protection and sustainability during the preparation process, such as avoiding the use of detergents containing chemical ingredients and promoting the concept of ecological planting; these measures help reduce pollution and damage to the environment and achieve sustainable utilization of resources.

[0087] Furthermore, the specific description of step S1 is as follows:

[0088] S1.1. Select plants that are vigorously growing and free of pests and diseases as raw materials, and give priority to plant varieties or parts with efficient exosome secretion capabilities, including specific leaves rich in bioactive ingredients, mature and plump fruits, and flowers with bright colors and strong fragrance. During the collection process, pay attention to avoid mechanical damage to the plants to maintain the integrity of the plant materials;

[0089] S1.2. Immediately clean the collected plant materials to remove dust, soil and other impurities on the surface; when cleaning, use clean water at an appropriate temperature and avoid using detergents containing chemical ingredients to avoid contaminating the plant materials or destroying their biologically active ingredients;

[0090] S1.3. Classify the plant materials after preliminary cleaning and subdivide them according to the type, growth part and intended use of the plant materials; for different types of plant materials, use targeted cleaning methods and tools to ensure the best cleaning effect;

[0091] S1.4. After classification and cleaning, further disinfect the plant materials; use ultraviolet disinfection, ozone disinfection or chemical disinfectants to effectively kill bacteria, viruses and other microorganisms on the surface of the plant materials to avoid the introduction of pollutants in the subsequent preparation process;

[0092] S1.5. Dry the disinfected plant materials to remove excess moisture. When drying, pay attention to maintaining appropriate temperature and humidity conditions to prevent the plant materials from losing their biologically active components due to excessive drying or from causing mildew due to excessive humidity.

[0093] S1.6. Finally, the quality of the dried plant materials is checked to ensure that the surface of the plant materials is clean, undamaged, and uncontaminated, and that they meet the requirements for preparing plant exosome-enriched nutrient solution; plant materials that do not meet the requirements are discarded or reprocessed.

[0094] It can be seen from the above that through the above steps, the cleanliness of the plant materials and the integrity of the bioactive components are ensured, and the preparation efficiency and quality of the plant exosome-rich nutrient solution are improved.

[0095] Furthermore, the specific description of step S2 is as follows:

[0096] S2.1. Use sharp knives or mechanical cutting equipment to finely cut the cleaned and pretreated plant materials into small pieces or slices. When cutting, pay attention to maintaining the consistency of the cutting size to ensure the effect of subsequent centrifugal separation. At the same time, avoid excessive damage to plant cells to reduce the generation of cell debris and impurities.

[0097] S2.2. Place the cut plant materials evenly into the centrifuge tube of the centrifuge to ensure that the plant materials in the centrifuge tube are evenly distributed to avoid local overload or imbalance during the centrifugation process;

[0098] S2.3. Select appropriate centrifugation speed and time according to the type of plant material, growth site and characteristics of bioactive components; for plant materials rich in exosomes, use a higher centrifugation speed and appropriate centrifugation time to fully separate the supernatant containing plant exosomes; at the same time, pay attention to monitoring the temperature changes during the centrifugation process to avoid high temperature from damaging the biological activity of plant exosomes;

[0099] S2.4. During the centrifugal separation process, dynamic monitoring technology is used to observe the stratification of the liquid in the centrifuge tube and the clarity of the supernatant in real time; when the supernatant becomes clear and the stratification is obvious, the centrifugal operation is stopped;

[0100] S2.5. Carefully remove the supernatant after centrifugation to avoid contact with the sediment at the bottom of the centrifuge tube; at the same time, perform a quality check on the removed supernatant to ensure that its clarity, purity and content of bioactive components meet the requirements for preparing plant exosome-enriched nutrient solution;

[0101] S2.6. For plant materials of different types and properties, record the optimal centrifugation speed and time parameters for reference and optimization in subsequent preparation processes.

[0102] From the above, it can be seen that this step not only improves the separation efficiency and purity of plant exosomes, but also ensures the stability and controllability of the preparation process.

[0103] Furthermore, pre-treating the plant material in a low-temperature environment for a short period of time before cutting it can help reduce the activity of enzymes in plant cells, prevent the degradation of exosomes during subsequent processing, and also help maintain the biological activity of exosomes.

[0104] Furthermore, before the cut plant material is placed in the centrifuge tube, it can be mixed with an appropriate amount of buffer or stabilizer. This step helps to maintain the stability of the exosomes during the centrifugation process and reduce their loss during the centrifugation process.

[0105] Furthermore, after the supernatant is removed, it can be subjected to secondary treatment, including ultrafiltration, dialysis or adsorption to further remove impurities and tiny particles. This step helps to improve the purity and biological activity of exosomes.

[0106] Furthermore, the specific description of step S3 is as follows:

[0107] S3.1. The supernatant obtained after centrifugation is first filtered through a coarse filter to remove larger impurities and cell debris; the pore size of the coarse filter is selected according to the particle size and distribution of impurities in the supernatant to ensure that most impurities can be effectively removed;

[0108] S3.2. The supernatant after the coarse filtration is further filtered through the medium filter to remove smaller impurities and cell debris; the pore size of the medium filter is finer than that of the coarse filter, which can ensure that the purity and transparency of the supernatant are further improved;

[0109] S3.3. In order to obtain a plant exosome solution with higher purity, the supernatant after the intermediate filtration is finally filtered through a precision filter; the pore size of the precision filter is selected according to the size and shape of the plant exosomes to ensure that tiny impurities and cell fragments can be effectively removed while retaining intact plant exosomes;

[0110] S3.4. During the filtration process, pressure control technology is used to ensure that the filtration pressure is moderate to avoid mechanical damage to plant exosomes or destruction of their biological activity. At the same time, filter blockage and filtration efficiency are monitored regularly, and clogged filters are replaced or filtration parameters are adjusted in a timely manner.

[0111] S3.5. Perform quality inspection on the plant exosome solution after multi-stage filtration, including indicators such as purity, transparency and content of bioactive components; ensure that the plant exosome solution meets the requirements for preparing plant exosome-enriched nutrient solution;

[0112] S3.6. In order to further improve the purity and stability of the plant exosome solution, membrane separation technologies such as ultrafiltration, nanofiltration or reverse osmosis can be used for further purification. Appropriate membrane separation technology and operating parameters can be selected according to the size and properties of the plant exosomes.

[0113] From the above, it can be seen that this step realizes multi-stage filtration and purification of the plant exosome solution, effectively removes impurities and cell debris, improves the purity and stability of the plant exosome solution, and provides high-quality raw materials for the subsequent preparation of plant exosome-enriched nutrient solution.

[0114] Furthermore, before coarse filtration, the supernatant is pre-filtered; that is, large impurities and suspended matter are quickly removed by using a coarser filter screen or gauze to reduce the burden on subsequent filters and improve filtration efficiency.

[0115] Furthermore, adjusting the pH of the supernatant according to the isoelectric point of plant exosomes before filtration can help change the surface charge of exosomes, making them easier to pass through the filter while reducing the adsorption of impurities and cell debris.

[0116] Furthermore, during the filtration process, the temperature of the supernatant was controlled to optimize the stability of the exosomes and the filtration efficiency.

[0117] Furthermore, after filtration, the plant exosome solution is further post-processed, including buffer exchange, concentration or dilution, to optimize its physiological environment and stability.

[0118] Furthermore, the specific description of step S4 is as follows:

[0119] S4.1. Collect the purified plant exosome solution obtained after multi-stage filtration into a sterile container that has been sterilized to ensure a sterile environment to avoid contamination by bacteria and other contaminants;

[0120] S4.2. According to the intended use of the plant exosome-enriched nutrient solution and the growth requirements of the target plants, a customized selection of suitable culture medium or nutrient solution is made; including but not limited to inorganic salts, organic nutrients, vitamins, trace elements, plant growth regulators and other ingredients;

[0121] S4.3. After determining the type of culture medium or nutrient solution, accurately calculate and adjust the concentration of each nutrient component according to the growth stage, growth rate and bioactive component requirements of the target plant; by adjusting the concentration of the nutrient components, the nutrient supply of the plant exosome-enriched nutrient solution can be optimized to promote the growth and development of the target plant;

[0122] S4.4. When adding culture medium or nutrient solution, gradually add and mix thoroughly to ensure uniform distribution of nutrients and avoid excessive or low local concentrations. At the same time, regularly monitor the pH value and osmotic pressure of the plant exosome-enriched nutrient solution to ensure that it is within an appropriate range.

[0123] S4.5. Conduct quality inspection on the prepared plant exosome-enriched nutrient solution, including indicators such as the content, uniformity and stability of nutrients; ensure that the plant exosome-enriched nutrient solution meets the expected requirements and has a good application effect;

[0124] S4.6. In order to further improve the adaptability and stability of the plant exosome-enriched nutrient solution, stability tests and adaptability assessments can be performed on it. Based on the test results and assessment results, the formula and nutritional components of the plant exosome-enriched nutrient solution can be optimized and adjusted to meet the needs of different plants and under different growth conditions.

[0125] From the above, it can be seen that this step realizes the customized formulation and nutritional adjustment of the plant exosome-enriched nutrient solution, improves its adaptability and stability, and provides high-quality plant exosome-enriched nutrient solution for applications in different plants and under different growth conditions.

[0126] Furthermore, in the process of adding culture medium or nutrient solution, dynamic adjustment technology is used to monitor and adjust the pH value and osmotic pressure of the plant exosome-enriched nutrient solution in real time to ensure that the nutrient solution is always kept within an appropriate range during the preparation process, thereby improving its stability and biological activity.

[0127] Furthermore, the specific description of step S5 is as follows:

[0128] S5.1. Conduct a comprehensive performance evaluation of the prepared plant exosome-enriched nutrient solution, including but not limited to indicators such as nutrient content, pH value, osmotic pressure, stability and biological activity; ensure that it can meet the growth needs and expected effects of the target plants in subsequent applications;

[0129] S5.2. Determine the types and quantities of other nutrients that need to be added based on the performance evaluation results and the growth requirements of the target plants; this includes amino acids, nucleotides, plant hormones, antioxidants or other bioactive substances, etc., to further improve the biological activity and nutritional value of the plant exosome-enriched nutrient solution;

[0130] S5.3. When adding other nutrients, use precise measurement and gradual addition to ensure that each ingredient can be evenly distributed in the plant exosome-enriched nutrient solution to avoid local concentrations that are too high or too low. At the same time, regularly monitor the composition changes and stability of the plant exosome-enriched nutrient solution and adjust the addition strategy in a timely manner.

[0131] S5.4. Accurately adjust the pH value of the plant exosome-rich nutrient solution to ensure that it is within the appropriate growth range; use a precision pH meter for monitoring and adjustment, and select appropriate acid-base regulators for adjustment based on the composition of the plant exosome-rich nutrient solution and the growth requirements of the target plants; at the same time, pay attention to avoid introducing new pollutants or destroying the biological activity of plant exosomes during the adjustment process;

[0132] S5.5. After adjusting the pH value, conduct another quality inspection and stability test on the plant exosome-enriched nutrient solution to ensure that it can maintain stable performance in subsequent applications and meet the growth requirements of the target plants;

[0133] S5.6. In order to further improve the adaptability and application range of plant exosome-enriched nutrient solution, its formula can be optimized and its performance improved; according to the feedback and test results in actual applications, the composition, concentration and preparation process of plant exosome-enriched nutrient solution can be adjusted to meet the needs of different plants and under different growth conditions.

[0134] From the above, it can be seen that this step realizes the fine regulation and performance enhancement of the plant exosome-enriched nutrient solution, improves its biological activity, nutritional value and adaptability, and provides a better quality plant exosome-enriched nutrient solution for applications in different plants and under different growth conditions.

[0135] Furthermore, high-throughput screening technology is introduced to conduct large-scale screening of potential bioactive ingredients, and their effectiveness is verified through in vitro experiments and plant physiological experiments to ensure that the newly added ingredients can significantly enhance the biological activity of plant exosome-enriched nutrient solution.

[0136] Furthermore, the plant exosome-enriched nutrient solution is post-processed using microbial fermentation technology, and the nutrients and bioactive substances in the nutrient solution are further transformed and enhanced through the metabolism of microorganisms.

[0137] Furthermore, the specific description of step S6 is as follows:

[0138] S6.1. Conduct a preliminary quality assessment on the prepared plant exosome-enriched nutrient solution to confirm that it meets the expected biological activity, nutritional value and stability requirements; only products that have passed strict quality control can be subsequently stored;

[0139] S6.2. Select appropriate storage containers to ensure that the plant exosome-rich nutrient solution will not be contaminated or lost during storage. The storage container should have good sealing, low temperature resistance and light-proof properties, and be able to withstand certain pressure changes to prevent damage from external factors.

[0140] S6.3. Set a suitable storage temperature to ensure that the plant exosome-rich solution is stored in a low temperature environment to slow down its degradation rate and prolong the storage time; the specific storage temperature should be determined based on the composition, concentration and stability test results of the plant exosome-rich solution, and it should be stored below 4°C;

[0141] S6.4. In addition to low-temperature storage, it is also necessary to pay attention to light-proof storage to prevent light from damaging the bioactive components in the plant exosome-rich nutrient solution. The storage container should be placed in an area where light cannot directly reach it, or be shielded with light-proof materials.

[0142] S6.5. Regularly check the quality of stored plant exosome-enriched nutrient solution, including testing its biological activity, nutritional value, stability and other indicators; if the quality is found to be degraded or not meeting the requirements, stop using it immediately and dispose of it;

[0143] S6.6. In order to further improve the storage stability of plant exosome-enriched nutrient solution, some advanced storage technologies can be used, such as vacuum packaging, freeze drying or modified atmosphere packaging; these technologies can effectively extend the shelf life of plant exosome-enriched nutrient solution and maintain its original quality and biological activity;

[0144] S6.7. Establish a complete storage record and tracking system to keep track of the storage status and usage history of the plant exosome-enriched nutrient solution; this will help to detect and resolve problems in a timely manner and ensure the safety and effectiveness of the plant exosome-enriched nutrient solution.

[0145] From the above, it can be seen that this step achieves the optimization of the storage conditions of the plant exosome-rich nutrient solution and the requirement of maintaining its quality for a long time, improves the stability and biological activity of the plant exosome-rich nutrient solution, and provides a more reliable guarantee for practical applications.

[0146] Furthermore, a dynamic monitoring system is introduced to monitor the temperature, humidity, light and other parameters in the storage environment in real time to ensure that they always remain within the set optimal range; once the environmental parameters exceed the set values, the system should immediately issue an alarm so that timely measures can be taken to make adjustments.

[0147] Furthermore, the preparation process of the plant exosome-rich nutrient solution is carried out under sterile conditions to avoid contamination by bacteria and other pollutants.

[0148] Control example: A traditional method for preparing plant exosome-enriched nutrient solution, comprising the following steps:

[0149] Step 1: Plant material collection and cleaning:

[0150] 1) Collect fresh or dried plant materials, such as leaves, fruits, flowers, etc.;

[0151] 2) preliminary cleaning of the plant material to remove dust, soil and other impurities on the surface;

[0152] Step 2: Mechanical crushing or homogenization:

[0153] The cleaned plant material is mechanically disrupted or homogenized to release the cellular contents therein;

[0154] Step 3: Preliminary separation:

[0155] The crushed plant material is initially separated by physical or chemical methods, such as centrifugation, sedimentation, filtration, etc., to remove larger cell fragments and other impurities;

[0156] Step 4: Exosome extraction:

[0157] Based on the initial separation, ultrafiltration, dialysis, density gradient centrifugation and other techniques were further used to extract plant exosomes;

[0158] Step 5: Exosome purification:

[0159] Purify the extracted plant exosomes to remove residual impurities and other non-exosome components;

[0160] Step 6: Preparation of nutrient solution:

[0161] Mixing the purified plant exosomes with an appropriate amount of culture medium or nutrient solution to prepare a plant exosome-rich nutrient solution;

[0162] Step 7: Save and apply:

[0163] Store the prepared plant exosome-rich nutrient solution under appropriate conditions for subsequent use.

[0164] The effect of the preparation method of the plant exosome-enriched nutrient solution of the embodiment was compared with the traditional preparation method of the plant exosome-enriched nutrient solution (control example), and the following table was obtained:

[0165]

[0166]

[0167] As can be seen from the above table, through the above comparison, it can be seen that the plant exosome enriched solution preparation method in the embodiment is superior to the traditional preparation method in terms of separation effect, separation speed, impurity content, shelf life and stability, operation standardization, and scope of application. This method is suitable for large-scale promotion and application.

[0168] In summary: The present invention significantly improves the preparation efficiency, component purity and biological activity retention ability of plant exosome-enriched nutrient solution through fine centrifugal separation, multi-stage filtration, customized formulation and optimized storage conditions, while enhancing its effect in practical applications, such as promoting plant growth and increasing yield, and pays attention to environmental protection and sustainability, providing a more efficient, reliable and environmentally friendly method for the preparation and application of plant exosome-enriched nutrient solution.

[0169] The embodiments of the present invention are provided for the purpose of illustration and description. Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A method for preparing a plant exosome-enriched nutrient solution, characterized in that: include: S1. Collect fresh plant materials, including but not limited to leaves, fruits, and flowers, and clean them; S2, cutting the cleaned plant material into small pieces, placing them in a centrifuge, and centrifuging them at an appropriate speed for a period of time to separate the supernatant containing plant exosomes; S3, filtering the supernatant after centrifugation through a filter to remove impurities and cell debris to obtain a pure plant exosome solution; S4, collecting the filtered plant exosome solution into a sterile container, and adding an appropriate amount of culture medium and nutrient solution to adjust the concentration of nutrients to obtain a plant exosome-rich nutrient solution; S5, then further processing the plant exosome-rich nutrient solution; S6. Store the prepared plant exosome-rich solution under appropriate conditions to maintain its stability and biological activity.

2. The method for preparing a plant exosome-enriched nutrient solution according to claim 1, characterized in that: The specific description of step S1 is as follows: S1.

1. Choose plants that are vigorous and free of pests and diseases as raw materials; S1.

2. Immediately clean the collected plant materials to remove dust, soil and other impurities on the surface; S1.

3. Classify the plant materials after preliminary cleaning and subdivide them according to the type of plant materials, growth part and intended use; S1.

4. On the basis of classification and cleaning, further disinfection of plant materials is carried out; S1.

5. Dry the sterilized plant material to remove excess water; S1.

6. Finally, the quality of the dried plant materials is checked.

3. The method for preparing a plant exosome-enriched nutrient solution according to claim 1, characterized in that: The specific description of step S2 is as follows: S2.

1. Use sharp knives and mechanical cutting equipment to finely cut the cleaned and pre-treated plant materials into small pieces or slices; S2.2, evenly place the cut plant materials into the centrifuge tube of the centrifuge; S2.

3. Select the appropriate centrifugation speed and time according to the type of plant material, growth site and characteristics of bioactive components; S2.

4. During the centrifugal separation process, dynamic monitoring technology is used to observe the stratification of the liquid in the centrifuge tube and the clarity of the supernatant in real time; S2.

5. Carefully remove the supernatant after centrifugation to avoid contact with the sediment at the bottom of the centrifuge tube; S2.

6. For plant materials of different types and properties, record the optimal centrifugation speed and time parameters.

4. The method for preparing a plant exosome-enriched nutrient solution according to claim 1, characterized in that: The specific description of step S3 is as follows: S3.1, the supernatant obtained after centrifugation is first filtered through a coarse filter to remove larger impurities and cell debris; S3.2, the supernatant after the coarse filtration is further filtered through the medium filter to remove smaller impurities and cell debris; S3.3, in order to obtain a plant exosome solution with higher purity, the filtered supernatant is finally filtered through a precision filter; S3.

4. Perform quality checks on the plant exosome solution after multi-stage filtration, including purity, transparency, and content of bioactive ingredients.

5. The method for preparing a plant exosome-enriched nutrient solution according to claim 1, characterized in that: The specific description of step S4 is as follows: S4.

1. Collect the purified plant exosome solution obtained after multi-stage filtration into a sterile container that has been sterilized; S4.

2. Customize and select appropriate culture medium and nutrient solution according to the intended use of the plant exosome-enriched nutrient solution and the growth requirements of the target plants; S4.

3. After determining the culture medium and nutrient solution, accurately calculate and adjust the concentration of each nutrient component according to the growth stage, growth rate and bioactive component requirements of the target plant; S4.

4. When adding culture medium and nutrient solution, gradually add and fully mix them. At the same time, regularly monitor the pH value and osmotic pressure index of the plant exosome-rich nutrient solution. S4.

5. Perform quality check on the prepared plant exosome-enriched nutrient solution.

6. The method for preparing a plant exosome-enriched nutrient solution according to claim 1, characterized in that: The specific description of step S5 is as follows: S5.

1. Conduct a comprehensive performance evaluation of the prepared plant exosome-enriched nutrient solution; S5.

2. Determine the types and quantities of other nutrients to be added based on the performance evaluation results and the growth requirements of the target plants; S5.3 When adding other nutrients, use precise measurement and gradual addition; S5.4, accurately adjust the pH value of the plant exosome-rich nutrient solution; S5.

5. After adjusting the pH value, the plant exosome-enriched nutrient solution is subjected to another quality inspection and stability test.

7. The method for preparing a plant exosome-enriched nutrient solution according to claim 1, characterized in that: The specific description of step S6 is as follows: S6.

1. Conduct a preliminary quality assessment of the prepared plant exosome-enriched nutrient solution to confirm that it meets the expected biological activity, nutritional value and stability requirements; S6.

2. Select appropriate storage containers; S6.

3. Set a suitable storage temperature to ensure that the plant exosome-rich fluid is stored in a low temperature environment; S6.

4. In addition to low-temperature storage, storage containers should be placed in an area where they cannot be directly exposed to light, or shielded with light-proof materials; S6.

5. Regularly check the quality of stored plant exosome-enriched nutrient solution.

8. A method for preparing a plant exosome-enriched nutrient solution according to any one of claims 1 to 7, characterized in that: The preparation process of the plant exosome-rich nutrient solution is carried out under sterile conditions to avoid contamination by bacteria and other pollutants.

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