Extraction method of aloe polysaccharide for cigarettes

Abstract

The invention relates to the technical field of aloe polysaccharide extraction, and provides an extraction method of aloe polysaccharide for cigarettes, which comprises the following steps: S1, crushing and filtering aloe leaves to obtain aloe juice; s2, adding an extracting agent into the aloe juice for extraction, and collecting an extracting solution; s3, an alcohol solvent is added into the extracting solution for precipitation, and aloe polysaccharide is obtained through recrystallization; the extracting agent comprises acetic acid, N, N-diethyl chloroformamide and water; the mass ratio of the acetic acid to the N, N-diethyl chloroformamide to the water is (1-6): 3: 2. According to the technical scheme, the problem that the extraction yield of the aloe polysaccharide is low in the prior art is solved.

Description

Technical Field

[0001] The invention relates to the technical field of aloe polysaccharide extraction, and in particular to a method for extracting tobacco aloe polysaccharide. Background Art

[0002] Aloe vera is known as a "panacea", and has unique effects in beauty, health care, food, etc. It is an important raw material in medicine, chemical industry, and food. As a pure natural green health product and natural therapy medicine, aloe vera is becoming more and more popular. Active ingredients in aloe vera are constantly being discovered, among which aloe polysaccharide is one of the main functional ingredients in aloe vera extraction. It has the function of promoting and enhancing the body's immunity, and also has anti-tumor, antioxidant, anti-ulcer and other effects. Aloe polysaccharide has a certain adsorption and wrapping ability. Adding it to tobacco shreds can absorb some irritating substances and odor components produced during tobacco combustion, thereby reducing the impurities and odors in the smoke, making the smoke more pure and natural. Traditional methods for extracting aloe polysaccharide include water extraction and enzyme extraction, but the yield of aloe polysaccharide prepared by these methods is low. Therefore, a high-yield aloe polysaccharide extraction method needs to be developed to meet the growing market demand. Summary of the invention

[0003] The invention provides a method for extracting tobacco aloe polysaccharide, which solves the problem of low extraction yield of aloe polysaccharide in the related art.

[0004] The technical solution of the present invention is as follows: The present invention provides a method for extracting tobacco aloe polysaccharide, comprising the following steps: S1, crushing and filtering aloe leaves to obtain aloe juice; S2, adding an extractant to the aloe juice for extraction, and collecting the extract; S3, adding an alcohol solvent to the extract to precipitate, and obtaining aloe polysaccharide by recrystallization; The extractant includes acetic acid, N,N-diethylchloroformamide, and water; The mass ratio of the acetic acid, N,N-diethylchloroformamide and water is 1-6:3:2.

[0005] As a further technical solution, the mass ratio of the acetic acid, N,N-diethylchloroformamide and water is 2-4:3:2.

[0006] In the present invention, the mass ratio of acetic acid, N,N-diethylchloroformamide and water in the extractant is limited to 2-4:3:2, thereby further improving the extraction yield of aloe polysaccharide.

[0007] As a further technical solution, the extraction temperature is 40-50°C, and the extraction time is 50-80 minutes.

[0008] As a further technical solution, the alcohol solvent includes one or more of ethanol, methanol, and n-butanol.

[0009] As a further technical solution, the recrystallization solvent includes ethanol and ethyl pyruvate.

[0010] In the present invention, the extraction yield and purity of aloe polysaccharide are further improved by limiting the solvent for recrystallization to include ethanol and ethyl pyruvate.

[0011] As a further technical solution, the volume ratio of the ethanol to the ethyl pyruvate is 19-24:1.

[0012] In the present invention, the volume ratio of ethanol to ethyl pyruvate in the recrystallization solvent is limited to 19-24:1, thereby further improving the extraction yield and purity of aloe polysaccharides.

[0013] As a further technical solution, in step S3, before adding the alcohol solvent to the extract for precipitation, the extract is also concentrated.

[0014] As a further technical solution, the concentration process is performed to 30% to 50% of the volume of the extract.

[0015] As a further technical solution, the precipitation temperature is 0-5°C, and the precipitation time is 1-2h.

[0016] The invention also provides an application of aloe polysaccharide prepared by a method for extracting tobacco aloe polysaccharide in tobacco.

[0017] The working principle and beneficial effects of the present invention are: In the invention, aloe leaves are used as raw materials, and after being crushed, an extractant consisting of acetic acid, N,N-diethylchloroformamide and water is added for extraction, and the mass ratio of acetic acid, N,N-diethylchloroformamide and water is limited to 1-6:3:2, so that the extraction yield of aloe polysaccharide is improved. After the prepared aloe polysaccharide is added to tobacco, the sweetness of the tobacco can be increased, the taste of the tobacco can be improved, and the smoke can be made softer and more comfortable. DETAILED DESCRIPTION

[0018] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] Example 1 A method for extracting tobacco aloe polysaccharide comprises the following steps: S1, wash 1 kg of aloe leaves and add them into a grinder for grinding, and then obtain aloe juice by vacuum filtration; S2, adding 2L of extractant to the aloe vera juice, heating to 40°C and extracting for 80 minutes, and collecting the extract after centrifugation; S3. Concentrate the extract to 30% of the original volume, add 2L of ethanol to the extract, precipitate at 0°C for 1h, filter out the precipitate, add the precipitate into 500mL of ethanol for recrystallization, and obtain 81.93g of aloe polysaccharide after filtration and drying, with a purity of 95.2% and a yield of 7.8%.

[0020] The extractant is obtained by mixing acetic acid, N,N-diethylchloroformamide and water in a mass ratio of 1:3:2.

[0021] Example 2 A method for extracting tobacco aloe polysaccharide comprises the following steps: S1, wash 1 kg of aloe leaves and add them into a grinder for grinding, and then obtain aloe juice by vacuum filtration; S2, adding 2L of extractant to the aloe vera juice, heating to 50°C and extracting for 50 minutes, and collecting the extract after centrifugation; S3. Concentrate the extract to 50% of the original volume, add 2L of n-butanol to the extract, precipitate at 5°C for 2h, filter out the precipitate, add the precipitate into 500mL of ethanol for recrystallization, and obtain 84.91g of aloe polysaccharide after filtering and drying, with a purity of 95.4% and a yield of 8.1%.

[0022] The extractant is obtained by mixing acetic acid, N,N-diethylchloroformamide and water in a mass ratio of 1:3:2.

[0023] Example 3 The only difference between this embodiment and embodiment 1 is that the extractant is obtained by mixing acetic acid, N,N-diethylchloroformamide and water in a mass ratio of 6:3:2.

[0024] Results: 85.86 g of aloe polysaccharide was obtained with a purity of 95.5% and a yield of 8.2%.

[0025] Example 4 The only difference between this embodiment and embodiment 1 is that the extractant is obtained by mixing acetic acid, N,N-diethylchloroformamide and water in a mass ratio of 2:3:2.

[0026] Results: 93.19 g of aloe polysaccharide was obtained with a purity of 95.5% and a yield of 8.9%.

[0027] Example 5 The present embodiment is different from the embodiment 1 only in that the extractant is obtained by mixing acetic acid, N,N-diethylchloroformamide and water in a mass ratio of 4:3:2.

[0028] Results: 94.34 g of aloe polysaccharide was obtained with a purity of 95.4% and a yield of 9.0%.

[0029] Example 6 The difference between this embodiment and embodiment 5 is that in step S3, the extract is concentrated to 30% of the original volume, 2L of ethanol is added to the extract, and precipitation is carried out at 0°C for 1h. After filtering out the precipitate, the precipitate is added to 500mL of a mixed solution of ethanol and ethyl pyruvate (the volume ratio of ethanol to ethyl pyruvate is 9:1) for recrystallization, and after filtering and drying, 95.19g of aloe polysaccharide is obtained with a purity of 97.7% and a yield of 9.3%.

[0030] Example 7 The only difference between this embodiment and embodiment 6 is that the volume ratio of ethanol to ethyl pyruvate is 30:1.

[0031] Results: 96.02 g of aloe polysaccharide was obtained with a purity of 97.9% and a yield of 9.4%.

[0032] Example 8 The only difference between this embodiment and embodiment 6 is that the volume ratio of ethanol to ethyl pyruvate is 19:1.

[0033] Results: 99.69 g of aloe polysaccharide was obtained with a purity of 98.3% and a yield of 9.8%.

[0034] Example 9 The only difference between this embodiment and embodiment 6 is that the volume ratio of ethanol to ethyl pyruvate is 24:1.

[0035] Results: 98.38 g of aloe polysaccharide was obtained with a purity of 98.6% and a yield of 9.7%.

[0036] Comparative Example 1 The only difference between this comparative example and Example 1 is that the extractant is obtained by mixing acetic acid and water in a mass ratio of 1:2.

[0037] Results: 73.56 g of aloe polysaccharide was obtained with a purity of 93.8% and a yield of 6.9%.

[0038] Comparative Example 2 The only difference between this comparative example and Example 1 is that the extractant is obtained by mixing acetic acid and N,N-diethylchloroformamide in a mass ratio of 1:3.

[0039] Results: 64.96 g of aloe polysaccharide was obtained with a purity of 93.9% and a yield of 6.1%.

[0040] Comparative Example 3 The only difference between this comparative example and Example 1 is that the extractant is acetic acid.

[0041] Results: 45.65 g of aloe polysaccharide was obtained with a purity of 94.2% and a yield of 4.3%.

[0042] Comparative Example 4 The only difference between this comparative example and Example 1 is that the extractant is water.

[0043] Results: 70.90 g of aloe polysaccharide was obtained with a purity of 94.5% and a yield of 6.7%.

[0044] Compared with Comparative Examples 1 to 4, the extractant in Example 1 consists of acetic acid, N,N-diethylchloroformamide and water. As a result, the yield of aloe polysaccharides prepared in Example 1 is higher than that in Comparative Examples 1 to 4, indicating that when the extractant consists of acetic acid, N,N-diethylchloroformamide and water, the yield of aloe polysaccharides can be improved.

[0045] Compared with Example 1, the mass ratio of the extractant was changed in Examples 3 to 5. As a result, the yield of aloe polysaccharides obtained in Examples 4 to 5 was higher than that in Example 1 and Example 3, indicating that when the mass ratio of acetic acid, N,N-diethylchloroformamide and water in the extractant was 2 to 4:3:2, the yield of aloe polysaccharides could be further improved.

[0046] Compared with Example 5, the recrystallization solvents in Examples 6 to 9 consisted of ethanol and ethyl pyruvate. As a result, the yield and purity of the aloe polysaccharides obtained in Examples 8 to 9 were higher than those in Examples 5 to 7, indicating that when the recrystallization solvent consisted of ethanol and ethyl pyruvate, and the volume ratio of ethanol to ethyl pyruvate was 19 to 24:1, the yield and purity of the aloe polysaccharides could be further improved.

[0047] Using 20v% ethanol aqueous solution as solvent, the aloe polysaccharide prepared in Example 1 was prepared into a 1wt% solution, 0.5g of the solution containing aloe polysaccharide was taken and sprayed on 100g of tobacco shreds, and rolled. Cigarettes without adding aloe polysaccharide were used as blank control group. The cigarettes were placed in a constant temperature and humidity chamber at a temperature of 22°C±1°C and a humidity of 60%±2% for 48h, and evaluated by professional smoking evaluation committee members in accordance with GB5606.4-2005 "Cigarettes Part 4: Sensory Technical Requirements". The evaluation standards and evaluation scoring results are shown in Tables 1~2.

[0048] Table 1 Cigarette sensory quality evaluation criteria

[0049] Table 2 Cigarette smoking sensory evaluation scoring results

[0050] Evaluation results: Example 1: The tobacco aroma is full and delicate, well-tuned with the tobacco flavor, free of impurities, non-irritating, and has a pure and comfortable aftertaste; Control group: The aroma of the tobacco is light and rough, and is in good harmony with the taste of tobacco. It has a slight smell and is more irritating, but the aftertaste is clean and comfortable.

[0051] The results of smoking test show that adding the aloe polysaccharide prepared in the present invention into shredded tobacco improves the taste of tobacco and makes the smoke purer and more comfortable.

[0052] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for extracting tobacco aloe polysaccharides, characterized in that: The following steps are involved: S1, crushing and filtering aloe leaves to obtain aloe juice; S2, adding an extractant to the aloe juice for extraction, and collecting the extract; S3, adding an alcohol solvent to the extract to precipitate, and obtaining aloe polysaccharide by recrystallization; The extractant includes acetic acid, N,N-diethylchloroformamide, and water; The mass ratio of the acetic acid, N,N-diethylchloroformamide and water is 1-6:3:

2.

2. The method for extracting tobacco aloe polysaccharides according to claim 1, characterized in that: The mass ratio of the acetic acid, N,N-diethylchloroformamide and water is 2-4:3:

2.

3. The method for extracting tobacco aloe polysaccharide according to claim 1, characterized in that: The extraction temperature is 40-50° C., and the extraction time is 50-80 min.

4. The method for extracting tobacco aloe polysaccharide according to claim 1, characterized in that: The alcohol solvent includes one or more of ethanol, methanol and n-butanol.

5. The method for extracting tobacco aloe polysaccharide according to claim 1, characterized in that: The recrystallization solvent includes ethanol and ethyl pyruvate.

6. The method for extracting tobacco aloe polysaccharide according to claim 5, characterized in that: The volume ratio of the ethanol to the ethyl pyruvate is 19-24:

1.

7. The method for extracting tobacco aloe polysaccharide according to claim 1, characterized in that: In step S3, before adding the alcohol solvent to the extract for precipitation, the extract is also concentrated.

8. The method for extracting tobacco aloe polysaccharide according to claim 7, characterized in that: The concentration process is performed to 30% to 50% of the volume of the extract.

9. The method for extracting tobacco aloe polysaccharide according to claim 1, characterized in that: The precipitation temperature is 0-5°C, and the precipitation time is 1-2h.

10. Use of the aloe polysaccharide prepared by the method for extracting aloe polysaccharide for tobacco according to any one of claims 1 to 9 in tobacco.

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