Preparation method and application of ganoderma lucidum mycelium leather
Through growth and metabolism regulation and natural polymer technology cross-linking, Ganoderma lucidum mycelium leather was prepared, which solved the problems of environmental pollution and resource consumption in traditional leather production, and achieved rapid, environmentally friendly and excellent performance bioleather production.
Patent Information
- Application Number
- CN202411307402.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-19
- Publication Date
- 2025-02-18
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The use of animal fur or petrochemical raw materials in the production process of existing leather leads to environmental pollution and resource consumption. The production cycle of traditional leather is relatively long, making it difficult to meet the rapidly developing market demand.
The tip growth of Ganoderma lucidum mycelium is promoted through growth and metabolism regulation, forming an interlocking structure to simulate the texture and texture of animal skin, and cross-linking is used to replace the traditional tanning process to prepare Ganoderma lucidum mycelium leather.
It has achieved bioleather production with zero cruelty, zero carbon, zero plastic and zero pollution, shortened the production cycle, reduced environmental pollution, and performed excellently in terms of mechanical and comprehensive performance.
Smart Images
Figure CN119463703A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to a preparation method of ganoderma mycelium leather and application thereof, belonging to the technical field of biotechnology and bio-based materials. Background Art
[0002] Ganoderma mycelium is the root tissue of Ganoderma lucidum. Mycelium is a natural and controllable biological material. After simple physical and chemical processing, the mycelium grown in organic waste can form textured and patterned mycelium leather. Compared with animal fur or petrochemical raw materials used in traditional leather production, mycelium has a series of advantages such as short growth cycle, low environmental requirements, and biodegradability.
[0003] We promote its tip growth by regulating growth metabolism. The modified Ganoderma mycelium can be used to make cruelty-free, carbon-free, plastic-free and pollution-free biological leather. Through growth metabolism regulation, it is allowed to entangle to achieve an interlocking structure to form the texture and feel of animal skin. The entire cultivation cycle only takes 7-10 days. Different from the back-end tanning of traditional leather, mycelium leather uses natural polymer technology for cross-linking. Compared with traditional leather, it avoids the use of strong acids, alkalis, heavy metals and other environmental pollutants, greatly reducing environmental pollution. Due to the unique composition of mycelium, it saves 1 / 2 of the process compared to traditional leather processing. The raw materials for feeding mushroom mycelium come from agricultural waste and are cultivated in a three-dimensional manner, so 80% of land area, water resources and carbon dioxide emissions are saved. The product will be used in automotive interiors, sustainable fashion, furniture and other industries. Summary of the invention
[0004] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides a method for preparing Ganoderma lucidum mycelium leather.
[0005] The invention provides a method for preparing ganoderma lucidum mycelium leather, which comprises the preparation of culture medium, regulation of growth metabolism and chemical modification of mycelium skin.
[0006] The culture medium preparation system comprises 100-200g corn stalk powder, 50-100g cottonseed hull powder, 10-20g soybean powder, 300-350g sawdust, 80-100g wheat bran, 1-3mg vitamin C and 300-400g purified water.
[0007] The regulation of growth metabolism comprises the following steps: S1: Spread the prepared culture medium evenly in a culture dish that can be sterilized by high pressure to a height of 0.5 cm, put it into a high pressure sterilizer, set the temperature to 121°C, and sterilize for 30 minutes. After the sterilization is completed, take it out for use; S2: Place the culture dish described in S1 in a biosafety cabinet, take 10g of Ganoderma lucidum spawn and sprinkle it in the culture dish, evenly covering the culture material; S3: Cover the culture dish described in S2 with a lid and place it in a biochemical incubator at a temperature of 30°C and a humidity of 90%, and take it out after 2 days of incubation; S4: remove the cover of the culture dish described in S3, place the culture dish in a three-gas incubator, set the carbon dioxide concentration to 6-8%, the oxygen concentration to 23-25%, the temperature to 25°C, and continue culturing for 8 days; The inoculated Ganoderma lucidum strains will form a dense fungus skin on the upper layer of the culture medium, wherein the water content of the fungus skin is greater than 80%.
[0008] After the cultivation is completed, the formed mycelium skin is removed and cleaned, and then immersed in a cross-linking modification solution, wherein the cross-linking modification solution is 50 ml of a 0.5% concentration catechin solution, and immersed at 20-30° C. for 2 hours.
[0009] After the polymer cross-linking modification is completed, the skin is taken out and cleaned, and the skin is immersed in 50 ml of plasticizing liquid, wherein the plasticizing liquid preparation system includes 20 ml of anhydrous ethanol solution, 10 ml of 1% concentration of corn protein solution, 5 ml of sulfonated castor oil, 5 ml of 0.5% concentration of sodium hyaluronate solution, 10 ml of 1% concentration of β-glucan solution, and immersed at 20-30°C for 2 hours.
[0010] After plasticization, take out the skin and clean it, place it in a well-ventilated environment, and let it dry naturally after 24 hours to form Ganoderma lucidum mycelium leather.
[0011] Preferably, the Ganoderma lucidum strain is Ganoderma lucidum.
[0012] Preferably, the culture medium preparation system is 100g corn straw powder, 100g cottonseed hull powder, 20g soybean powder, 300g sawdust, 80g wheat bran, 3mg vitamin C, and 400g purified water.
[0013] Preferably, the carbon dioxide concentration in step S4 is 8%.
[0014] Preferably, the oxygen concentration in step S4 is 25%.
[0015] Preferably, the cross-linking modification temperature is 30°C.
[0016] Preferably, the plasticizing modification temperature is 30°C.
[0017] The invention also discloses a ganoderma mycelium leather prepared by the method.
[0018] Beneficial Effects (1) The present invention provides a method for preparing Ganoderma lucidum mycelium leather, which can greatly simplify the operation, reduce the cost, shorten the culture cycle, and improve the growth efficiency of the mycelium, and is suitable for large-scale and three-dimensional production of Ganoderma lucidum mycelium leather; (2) The water vapor permeability of the Ganoderma lucidum mycelium leather prepared by the method of the present invention is 20-50 mg / m 2 h, bending resistance is 30,000-50,000 times, mechanical strength is 50-100MPa, and comprehensive performance is excellent. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 This is a photo of the Ganoderma mycelium obtained according to the method described in Example 1.
[0020] Figure 2 This is a scanning electron microscope photograph of the Ganoderma lucidum mycelium leather obtained according to the method described in Example 2. DETAILED DESCRIPTION
[0021] The present invention will be further described in the following examples, but the present invention is not limited thereto.
[0022] Example 1: Preparation and testing of Ganoderma lucidum mycelium leather 1) Culture medium preparation system: 100g corn straw powder, 100g cottonseed hull powder, 20g soybean powder, 300g sawdust, 80g wheat bran, 3mg vitamin C, 400g purified water, add into a mixer, set the speed to 180rpm, mix for 10min, spread the prepared culture medium evenly in a culture dish that can be sterilized by autoclave, with a height of 0.5cm, put it into an autoclave, set the temperature to 121℃, sterilize for 30min, take it out for use after sterilization; 2) Regulation of growth metabolism: Place the culture dish in a biosafety cabinet, take 10g of Ganoderma lucidum and sprinkle it in the culture dish, evenly cover the culture material, cover the culture dish, and place it in a biochemical incubator at a temperature of 30°C and a humidity of 90%. Take it out after 2 days of incubation, remove the cover of the culture dish, and place the culture dish in a three-gas incubator with a carbon dioxide concentration of 8%, an oxygen concentration of 25%, and a temperature of 25°C. Continue to incubate for 8 days, take a small piece of Ganoderma lucidum skin and observe it under a microscope. Figure 1 As shown; 3) Chemical modification of mycelium skin: After the cultivation is completed, the formed mycelium skin is removed and cleaned, and then immersed in 50 ml of 0.5% catechin solution at 30°C for 2 hours, and then removed and cleaned after the immersion is completed. The skin is immersed in 50 ml of plasticizing solution at 30°C for 2 hours, and then removed and cleaned after the immersion is completed. After natural air drying, the mycelium leather is formed; 4) Ganoderma mycelium leather test: The water vapor permeability test was conducted using the GB / T 1811-1993 leather water vapor permeability test method. The bending resistance test was conducted using the method described in GB / T 4689.9-1984. The mechanical properties test was conducted by cutting the sample into 1×5cm long strips and conducting a tensile test on a universal material testing machine at a stretching speed of 5cm / min. The water vapor permeability of the Ganoderma leather was measured to be 50mg / m2·h, the bending resistance was 50,000 times, and the mechanical strength was 100MPa.
[0023] Example 2: Preparation and testing of Ganoderma lucidum mycelium leather 1) Culture medium preparation system: 100g corn straw powder, 50g cottonseed hull powder, 20g soybean powder, 400g sawdust, 80g wheat bran, 1mg vitamin C, 350g purified water, add to the mixer, set the speed to 200rpm, mix for 5min, evenly spread the prepared culture medium in a culture dish that can be sterilized by high pressure, with a height of 0.5cm, put it in the autoclave, set the temperature to 121℃, sterilize for 30min, and take it out for use after sterilization; 2) Regulation of growth metabolism: Place the culture dish in a biosafety cabinet, take 10g of Ganoderma lucidum and sprinkle it in the culture dish, evenly cover the culture medium, cover the culture dish, and place it in a biochemical incubator at a temperature of 30°C and a humidity of 90%. Take it out after 2 days of incubation, remove the cover of the culture dish, and place the culture dish in a three-gas incubator with a carbon dioxide concentration of 6%, an oxygen concentration of 23%, and a temperature of 25°C. Continue incubation for 8 days; 3) Chemical modification of mycelium skin: After the cultivation is completed, the mycelium skin formed is removed and cleaned, and then immersed in 50 ml of 0.5% catechin solution at 30°C for 2 hours. After the immersion, it is removed and cleaned. The skin is immersed in 50 ml of plasticizing solution at 30°C for 2 hours. After the immersion, it is removed and cleaned. After natural air drying, Ganoderma lucidum mycelium leather is formed. Take a small piece of mycelium leather and observe it under a scanning electron microscope. Figure 2 As shown; 4) Ganoderma lucidum mycelium leather test: The water vapor permeability test was conducted using the leather water vapor permeability test method GB / T 1811-1993. The bending resistance test was conducted using the method described in GB / T 4689.9-1984. The mechanical properties test was conducted by cutting the sample into 1×5cm long strips and conducting a tensile test on a universal material testing machine at a stretching speed of 5cm / min. The water vapor permeability of the Ganoderma lucidum leather was measured to be 25mg / m2·h, the bending resistance was 35,000 times, and the mechanical strength was 78MPa.
[0024] Example 3: Preparation and testing of Ganoderma lucidum mycelium leather 1) Culture medium preparation system: 100g corn straw powder, 100g cottonseed hull powder, 20g soybean powder, 300g sawdust, 80g wheat bran, 3mg vitamin C, 400g purified water, add into a mixer, set the speed to 180rpm, mix for 10min, spread the prepared culture medium evenly in a culture dish that can be sterilized by autoclave, with a height of 0.5cm, put it into an autoclave, set the temperature to 121℃, sterilize for 30min, take it out for use after sterilization; 2) Regulation of growth metabolism: Place the culture dish in a biosafety cabinet, take 10g of Ganoderma lucidum and sprinkle it in the culture dish, evenly cover the culture medium, cover the culture dish, and place it in a biochemical incubator at a temperature of 30°C and a humidity of 90%. Take it out after 2 days of incubation, remove the cover of the culture dish, and place the culture dish in a three-gas incubator with a carbon dioxide concentration of 7%, an oxygen concentration of 24%, and a temperature of 25°C. Continue incubation for 8 days; 3) Chemical modification of mycelium skin: After the cultivation is completed, the formed mycelium skin is removed and cleaned, and then immersed in 50 ml of 0.5% catechin solution at 25°C for 2 hours, and then removed and cleaned after the immersion is completed. The skin is immersed in 50 ml of plasticizing solution at 25°C for 2 hours, and then removed and cleaned after the immersion is completed. After natural air drying, the mycelium leather is formed; 4) Ganoderma lucidum mycelium leather test: The water vapor permeability test was conducted using the leather water vapor permeability test method GB / T 1811-1993. The bending resistance test was conducted using the method described in GB / T 4689.9-1984. The mechanical properties test was conducted by cutting the sample into 1×5cm long strips and conducting a tensile test on a universal material testing machine at a stretching speed of 5cm / min. The water vapor permeability of the Ganoderma lucidum leather was measured to be 41mg / m2·h, the bending resistance was 41,000 times, and the mechanical strength was 92MPa.
[0025] The preparation method and application of the Ganoderma mycelium leather disclosed and proposed in the present invention can be realized by those skilled in the art by referring to the content of this article and appropriately changing the conditions, routes and other links. Although the method and preparation technology of the present invention have been described through preferred embodiments, relevant technicians can obviously modify or re-combine the methods and technical routes described herein without departing from the content, spirit and scope of the present invention to achieve the final preparation technology. It should be particularly pointed out that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the spirit, scope and content of the present invention.
Claims
1. A method for preparing Ganoderma lucidum mycelium leather, wherein the method comprises the following steps: as follows: 1) Preparation of culture medium: Take 100g corn straw powder, 100g cottonseed hull powder, 20g soybean powder, 300g sawdust, 80g wheat bran, 1mg vitamin C, and 400g purified water, add them into a mixer, set the speed to 150-200rpm, and mix for 5-10min. Spread the prepared culture medium evenly in a culture dish that can be sterilized by high pressure, with a height of 0.5cm. Put it into a high pressure sterilizer, set the temperature to 121℃, and sterilize for 30min. Take it out after sterilization and set it aside for use. 2) Regulation of growth metabolism: Place the culture dish in a biosafety cabinet, take 10g of Ganoderma lucidum and sprinkle it in the culture dish, evenly cover the culture medium, cover the culture dish, and place it in a biochemical incubator at a temperature of 30°C and a humidity of 90%. Take it out after 2 days of incubation, remove the cover of the culture dish, and place the culture dish in a three-gas incubator with carbon dioxide concentration of 6-8%, oxygen concentration of 23-25%, and temperature of 25°C. Continue incubation for 8 days. 2.3) Chemical modification of mycelium skin: After the cultivation is completed, the formed mycelium skin is removed and cleaned, and then immersed in 50 ml of 0.5% catechin solution at 20-30°C for 2 hours. After the immersion, it is removed and cleaned. The skin is immersed in 50 ml of plasticizing solution at 20-30°C for 2 hours. After the immersion, it is removed and cleaned. After natural air drying, the Ganoderma lucidum mycelium leather is formed.
3. The culture medium preparation system according to claim 1, characterized in that: The culture medium preparation system comprises 100-200g corn stalk powder, 50-100g cottonseed hull powder, 10-20g soybean powder, 300-350g sawdust, 80-100g wheat bran, 1-3mg vitamin C and 300-400g purified water.
4. The plasticizing liquid preparation system according to claim 1, characterized in that: The plasticizing liquid preparation system includes 20 ml of anhydrous ethanol solution, 10 ml of 1% corn protein solution, 5 ml of sulfonated castor oil, 5 ml of 0.5% sodium hyaluronate solution, and 10 ml of 1% β-glucan solution.
5. The Ganoderma lucidum strain according to claim 1, characterized in that: The ganoderma lucidum strain is purple ganoderma lucidum, and the sample strain is collected from the wild.