A method and device for detecting collagen

Abstract

The present invention relates to the field of spectrum detection technology, and in particular to a collagen detection method and device. The method obtains an infrared spectrum of the collagen to be detected; based on the distribution of absorption peaks in the infrared spectrum, the target absorption peak generated by non-noise is screened out; according to the position of the band corresponding to the target absorption peak, the type of collagen corresponding to each target absorption peak is obtained; according to the area of ​​all target absorption peaks corresponding to each collagen type, the content of each collagen type is obtained; based on the content of each collagen type, the function of the collagen to be detected is determined. The present invention avoids the confusion of collagen types by accurately determining the collagen type corresponding to the target absorption peak, so that the content of each collagen type is accurately obtained, and then the function of the collagen to be detected is accurately analyzed.

Description

Technical Field

[0001] The present invention relates to the technical field of spectral detection, and in particular to a collagen detection method and a device thereof. Background Art

[0002] Collagen is an important structural protein and one of the most abundant proteins in the human body. It is widely present in connective tissues such as skin, bones, muscles, blood vessels, and cartilage. It mainly supports, strengthens, and maintains tissue structure. Collagen molecules are composed of three polypeptide chains bonded together by hydrogen bonds, forming a triple helix structure that gives collagen strong stretchability and toughness, and can withstand greater pressure and tension. There are many types of collagen depending on their distribution and function, and different types of collagen have different functions. In order to study the role of collagen in different diseases and help early diagnosis and monitoring of disease progression, it is necessary to accurately analyze the type of collagen in the collagen to be tested and the content of each type of collagen, and then determine the specific function of the collagen to be tested.

[0003] It is known that the chemical bonds of each type of collagen absorb infrared light within a specific wavelength range. Therefore, the existing method uses infrared spectroscopy to determine the type of collagen in the collagen to be tested and the content of each type of collagen. However, in actual situations, the infrared light absorption bands corresponding to the chemical bonds of different types of collagen are similar, which makes it impossible to accurately distinguish the absorption peaks corresponding to the various types of collagen in the collagen to be tested, making it impossible to determine the content of each type of collagen, and thus it is impossible to accurately infer the function of the collagen to be tested. Summary of the invention

[0004] In order to solve the technical problem that the absorption peaks corresponding to various collagen types in the collagen to be detected cannot be accurately distinguished by infrared spectroscopy, making it impossible to determine the content of each collagen type, and thus unable to accurately infer the function of the collagen to be detected, the purpose of the present invention is to provide a collagen detection method and device, and the technical scheme adopted is as follows:

[0005] In a first aspect, an embodiment of the present invention provides a method for detecting collagen, the method comprising the following steps:

[0006] Obtaining an infrared spectrum of the collagen to be detected;

[0007] Filter out the target absorption peaks not generated by noise based on the distribution of absorption peaks in the infrared spectrum;

[0008] According to the position of the band corresponding to the target absorption peak, the type of collagen corresponding to each target absorption peak is obtained;

[0009] According to the area of ​​all target absorption peaks corresponding to each collagen type, the content of each collagen type is obtained;

[0010] The function of the collagen to be tested is determined based on the amount of each collagen type.

[0011] Furthermore, the method for obtaining the target absorption peak is:

[0012] The absorbance corresponding to each wave number in the infrared spectrum is clustered by the ISODATA clustering algorithm to obtain the clustering cluster of each absorption peak in the infrared spectrum;

[0013] According to the distribution of the absorbance in the cluster of each absorption peak and the wave number corresponding to the absorbance, the non-noise degree of each absorption peak is obtained;

[0014] When the non-noise level is greater than or equal to a preset non-noise level threshold, the corresponding absorption peak is used as a target absorption peak.

[0015] Furthermore, the method for obtaining the non-noise degree is:

[0016] For any absorption peak, the waveband consisting of the wavenumber corresponding to the absorbance in the cluster of the absorption peak is used as the reference waveband of the absorption peak;

[0017] The ratio of the maximum absorbance in the cluster of the absorption peak to the length of the reference band is taken as the prominence of the absorption peak;

[0018] Obtaining the mean square error of all absorbances in the cluster of the absorption peak as the first rule reference value of the waveform of the absorption peak;

[0019] The reference band is divided using the wave number corresponding to the absorption peak as a dividing point to obtain two local bands;

[0020] Obtaining the difference in area between the two local bands corresponding to the infrared spectrum as a second rule reference value of the waveform of the absorption peak;

[0021] The result of negatively correlating the product of the first waveform rule reference value and the second waveform rule reference value is used as the waveform rule degree of the absorption peak;

[0022] The result of normalizing the product of the prominence of the absorption peak and the regularity of the waveform is taken as the non-noise degree of the absorption peak.

[0023] Furthermore, the method for obtaining the type of collagen corresponding to each target absorption peak according to the position of the waveband corresponding to the target absorption peak is:

[0024] Obtaining the standard absorption wavenumber corresponding to each chemical bond of each collagen type; wherein the chemical bonds include NH bonds, C=O bonds, CH bonds, and CN bonds, and the collagen types include type I, type II, and type III;

[0025] According to the position distribution between each standard absorption wave number and the reference band of each target absorption peak, the adjacent target absorption peak of each standard absorption wave number is obtained;

[0026] The standard absorption wavenumbers corresponding to the NH bond and the C=O bond of each collagen type are the same, and the standard absorption wavenumbers corresponding to the NH bond and the C=O bond are all used as the first standard absorption wavenumber. For any first standard absorption wavenumber, the first preset number of target absorption peaks whose corresponding wavenumbers are closest to the first standard absorption wavenumber among the adjacent target absorption peaks of the first standard absorption wavenumber are used as reference absorption peaks of the first standard absorption wavenumber;

[0027] Wherein, when the total number of adjacent target absorption peaks of the first standard absorption wavenumber is greater than or equal to the number of types of collagen, the first preset number is the number of types of collagen; when the total number of adjacent target absorption peaks of the first standard absorption wavenumber is less than the number of types of collagen, the first preset number is the total number of adjacent target absorption peaks of the standard absorption wavenumber;

[0028] Obtaining the type of collagen corresponding to each reference absorption peak of the first standard absorption wavenumber according to the difference in absorbance between each reference absorption peak of the first standard absorption wavenumber and other reference absorption peaks;

[0029] The standard absorption wavenumber corresponding to the CH bond of each collagen type is the same, the standard absorption wavenumber corresponding to the CH bond is used as the second standard absorption wavenumber, and the collagen type corresponding to each reference absorption peak of the second standard absorption wavenumber is obtained according to the length of the reference band of each reference absorption peak of the second standard absorption wavenumber;

[0030] The standard absorption wavenumbers corresponding to the CN bonds of type II collagen and type III collagen are the same, and the standard absorption wavenumbers corresponding to the CN bonds of type I collagen and type II collagen are different. The standard absorption wavenumber corresponding to the CN bond of type I collagen is used as the third standard absorption wavenumber, and the standard absorption wavenumber corresponding to the CN bond of type II collagen and type III collagen is used as the fourth standard absorption wavenumber;

[0031] The collagen type corresponding to the target absorption peak whose wave number is closest to the third standard absorption wave number among the target absorption peaks adjacent to the third standard absorption wave number is set as type I;

[0032] The two target absorption peaks whose corresponding wavenumbers are closest to the fourth standard absorption wavenumber among the target absorption peaks adjacent to the fourth standard absorption wavenumber are used as matching absorption peaks of the fourth standard absorption wavenumber;

[0033] Obtaining the absorbance of each matching absorption peak as the first characteristic value of the corresponding matching absorption peak;

[0034] The collagen type corresponding to the matching absorption peak corresponding to the largest first eigenvalue is set as type II;

[0035] The collagen type corresponding to the matching absorption peak corresponding to the smallest first eigenvalue is set as type III;

[0036] When there is one adjacent target absorption peak of the fourth standard absorption wave number, the collagen type corresponding to the adjacent target absorption peak of the fourth standard absorption wave number is set to type II.

[0037] Furthermore, the method for obtaining the adjacent target absorption peak is:

[0038] For any standard absorption wave number and any target absorption peak, the left boundary wave number of the reference band of the target absorption peak is taken as the first wave number of the target absorption peak;

[0039] The right boundary wave number of the reference band of the target absorption peak is used as the second wave number of the target absorption peak;

[0040] The difference between the first wave number and the standard absorption wave number is taken as the first difference;

[0041] The difference between the second wave number and the standard absorption wave number is taken as the second difference;

[0042] Normalizing the product of the first difference and the second difference as the approximation degree between the positions of the target absorption peak and the standard absorption wave number;

[0043] When the position approximation is less than a preset position approximation threshold, the corresponding target absorption peak is used as the adjacent target absorption peak of the standard absorption wave number.

[0044] Furthermore, the method for obtaining the type of collagen corresponding to each reference absorption peak of the first standard absorption wavenumber according to the difference in absorbance between each reference absorption peak of the first standard absorption wavenumber and other reference absorption peaks is:

[0045] For any reference absorption peak of the first standard absorption wavenumber, obtaining the difference between the absorbance of the reference absorption peak and each other reference absorption peak of the first standard absorption wavenumber, and taking them as the reference difference value of the reference absorption peak;

[0046] The accumulated result of the reference difference values ​​of the reference absorption peak is used as the peak intensity of the reference absorption peak;

[0047] Obtaining the peak intensity of each reference absorption peak of the first standard absorption wavenumber, when the number of reference absorption peaks of the first standard absorption wavenumber is equal to the number of collagen types, setting the collagen type corresponding to the reference absorption peak corresponding to the largest peak intensity as type I; setting the collagen type corresponding to the reference absorption peak corresponding to the second largest peak intensity as type II; setting the collagen type corresponding to the reference absorption peak corresponding to the smallest peak intensity as type III;

[0048] When the number of reference absorption peaks of the first standard absorption wavenumber is less than the number of types of collagen, and the number of reference absorption peaks of the first standard absorption wavenumber is 2, the collagen type corresponding to the reference absorption peak corresponding to the maximum peak intensity is set to type I; the collagen type corresponding to the reference absorption peak corresponding to the minimum peak intensity is set to type II;

[0049] When the number of reference absorption peaks of the first standard absorption wavenumber is less than the number of collagen types and the number of reference absorption peaks of the first standard absorption wavenumber is 1, the collagen type corresponding to the reference absorption peak of the first standard absorption wavenumber is set to type I.

[0050] Furthermore, the method for obtaining the type of collagen corresponding to each reference absorption peak of the second standard absorption wavenumber according to the size of the reference waveband of each reference absorption peak of the second standard absorption wavenumber is:

[0051] The length of the reference waveband of each reference absorption peak of the second standard absorption wavenumber is taken as the first length;

[0052] When the number of reference absorption peaks of the second standard absorption wavenumber is equal to the number of collagen types, the collagen type corresponding to the reference absorption peak corresponding to the largest first length is set to type II; the collagen type corresponding to the reference absorption peak corresponding to the second largest first length is set to type I; the collagen type corresponding to the reference absorption peak corresponding to the smallest first length is set to type III;

[0053] When the number of reference absorption peaks of the second standard absorption wavenumber is less than the number of types of collagen, and the number of reference absorption peaks of the second standard absorption wavenumber is 2, the collagen type corresponding to the reference absorption peak corresponding to the largest first length is set to type II; the collagen type corresponding to the reference absorption peak corresponding to the smallest first length is set to type I;

[0054] When the number of reference absorption peaks of the second standard absorption wavenumber is less than the number of collagen types and the number of reference absorption peaks of the second standard absorption wavenumber is 1, the collagen type corresponding to the reference absorption peak of the second standard absorption wavenumber is set to type I.

[0055] Furthermore, the method for obtaining the area of ​​the target absorption peak is:

[0056] For any target absorption peak, the area of ​​the region corresponding to the reference band of the target absorption peak in the infrared spectrum image is taken as the area of ​​the target absorption peak.

[0057] Furthermore, the method for obtaining the content of each collagen type is:

[0058] For any type of collagen, the area accumulation of all target absorption peaks corresponding to the type of collagen is normalized and the result is taken as the content of the type of collagen.

[0059] In a second aspect, another embodiment of the present invention provides a collagen detection device, which includes: a memory, a processor, and a computer program stored in the memory and running on the processor, and when the processor executes the computer program, the steps of any one of the above methods are implemented.

[0060] The present invention has the following beneficial effects:

[0061] The present invention firstly screens out target absorption peaks not generated by noise based on the distribution of absorption peaks in an infrared spectrum, thereby avoiding interference from absorption peaks generated by noise, which is beneficial to the subsequent accurate analysis of the function of the collagen to be detected; then, according to the position of the band corresponding to the target absorption peak, the type of collagen corresponding to each target absorption peak is obtained, thereby avoiding confusion of collagen types and improving the accuracy and reliability of the detection of the collagen to be detected; then, according to the area of ​​all target absorption peaks corresponding to each collagen type, the content of each collagen type is obtained, the function of the collagen to be detected is accurately determined, accurate data support is provided for related medical diagnosis, and it is helpful for early diagnosis of the disease and monitoring of the progression of the disease. BRIEF DESCRIPTION OF THE DRAWINGS

[0062] In order to more clearly illustrate the technical solutions and advantages in the embodiments of the present invention or the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0063] Figure 1A schematic flow chart of a collagen detection method provided by one embodiment of the present invention;

[0064] Figure 2 A flow chart of a method for obtaining a target absorption peak provided by one embodiment of the present invention;

[0065] Figure 3 A structural diagram of a collagen detection system provided by one embodiment of the present invention;

[0066] Figure 4 A schematic diagram of a computer device provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0067] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following is a detailed description of a collagen detection method and device according to the present invention, its specific implementation, structure, characteristics and effects, in combination with the accompanying drawings and preferred embodiments. In the following description, different "one embodiment" or "another embodiment" does not necessarily refer to the same embodiment. In addition, specific features, structures or characteristics in one or more embodiments may be combined in any suitable form.

[0068] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0069] The specific scheme of a collagen detection method and device provided by the present invention is described in detail below with reference to the accompanying drawings.

[0070] Embodiment 1:

[0071] The present invention proposes a collagen detection method, please refer to Figure 1 , which shows a schematic flow chart of a collagen detection method provided by an embodiment of the present invention, the method comprising the following steps:

[0072] Step S1: Obtaining the infrared spectrum of the collagen to be detected.

[0073] Specifically, collagen is generally formed by three polypeptide chains intertwined through a triple helix structure. Due to its unique three-dimensional structure, collagen has multiple absorption peaks in the infrared spectrum, which are related to the vibration of different chemical bonds in its molecules, wherein chemical bonds include NH bonds, C=O bonds, CH bonds, and CN bonds. The difference in the complexity of the three-dimensional structure of collagen will produce different types of collagen. The types of collagen in this embodiment include type I, type II, and type III. Among them, type I collagen is the most common, mainly found in skin, bones, tendons, and teeth, and it is the main source of skin elasticity and strength; type II collagen is mainly found in cartilage, responsible for supporting joints and maintaining the structural integrity of joints; type III collagen is mainly found in the connective tissue of the skin, blood vessel walls, and internal organs, usually with type I collagen, to provide tissue elasticity. It is known that different types of collagen have different functions. In order to accurately analyze the functions of collagen and study the role of collagen in different diseases, which is helpful for early diagnosis of diseases and monitoring of disease progression, the existing methods can identify and quantitatively analyze the chemical bonds and molecular vibration information in collagen through infrared spectroscopy, thereby providing strong support for obtaining its structure and function. However, there is confusion in the absorption peaks corresponding to the chemical bonds of different types of collagen in the infrared spectrum, which makes it impossible to accurately analyze the content of each type of collagen in collagen, and thus it is impossible to accurately analyze the function of collagen. Among them, infrared spectroscopy is a well-known technology and will not be described in detail.

[0074] In order to accurately analyze the function of collagen, this embodiment uses a Fourier transform infrared spectrometer (FTIR) to obtain an infrared spectrum of the collagen to be detected. Subsequently, the absorption peaks in the infrared spectrum are analyzed to determine the type of collagen in the collagen to be detected, and then the content of each type of collagen is determined to accurately analyze the function of the collagen to be detected. Among them, this embodiment sets the wavelength range of the Fourier transform infrared spectrometer to to This range can cover most of the important molecular vibration modes in collagen, and the resolution is set to , ensuring that different vibration bands can be distinguished to obtain corresponding infrared spectra. The implementer can set the wavelength range and resolution according to the actual situation, which is not limited here.

[0075] Step S2: Filter out target absorption peaks that are not generated by noise based on the distribution of absorption peaks in the infrared spectrum.

[0076] Specifically, some absorption peaks in the infrared spectrum may be caused by the noise caused by the measurement error of the Fourier transform infrared spectrometer itself or other impurities, which in turn affects the analysis of the type and function of collagen. In order to improve the accuracy of qualitative and quantitative analysis of the collagen molecules to be detected, this embodiment needs to screen out the target absorption peaks that are not generated by noise. It is known that the target absorption peaks corresponding to the chemical bonds in collagen in the infrared spectrum usually have a smooth and symmetrical shape, showing a Gaussian shape, and the corresponding peak intensity is relatively high, while the absorption peaks formed by noise are often irregular and randomly distributed, and usually do not form a smooth curve, and the corresponding peak intensity is relatively low. Therefore, this embodiment screens out the target absorption peaks that are not generated by noise based on the distribution of the absorption peaks in the infrared spectrum.

[0077] Preferably, in one possible implementation of this embodiment, the method for obtaining the target absorption peak is as follows: Figure 2 , which shows a flow chart of a method for obtaining a target absorption peak provided in this embodiment, the method comprising the following steps:

[0078] Step S201: clustering the absorbance corresponding to each wave number in the infrared spectrum using the ISODATA clustering algorithm to obtain a cluster of each absorption peak in the infrared spectrum.

[0079] The ISODATA clustering algorithm is a well-known technology and will not be described in detail. It should be noted that the absorbance in each absorption peak cluster is essentially composed of the absorbance corresponding to each absorption peak and its local absorbance, which is helpful for analyzing whether each absorption peak is generated by noise.

[0080] Step S202: Obtain the non-noise degree of each absorption peak according to the distribution of the absorption rate in the cluster of each absorption peak and the wave number corresponding to the absorption rate.

[0081] When the shape of the absorbance in the cluster of a certain absorption peak is smooth and symmetrical according to the corresponding wave number in the infrared spectrum, and the more obvious the peak is, the less likely it is that the absorption peak is generated by noise. In this embodiment, the non-noise degree of each absorption peak is obtained according to the distribution of the absorbance in the cluster of each absorption peak and the wave number corresponding to the absorbance. The greater the non-noise degree, the more likely the corresponding absorption peak is the absorption peak corresponding to collagen.

[0082] Preferably, in a method that can be implemented in this embodiment, the method for obtaining the non-noise degree is: for any absorption peak, the band consisting of the wavenumber corresponding to the absorbance in the cluster of the absorption peak is used as the reference band of the absorption peak; the ratio of the maximum absorbance in the cluster of the absorption peak to the length of the reference band is used as the prominence of the absorption peak; the greater the prominence, the more obvious the absorption peak is in the infrared spectrum, and the more likely the absorption peak is the target absorption peak corresponding to collagen; wherein the maximum absorbance in the cluster of the absorption peak is the absorbance corresponding to the absorption peak;

[0083] The mean square error of all absorbances in the cluster of the absorption peak is obtained as the waveform first rule reference value of the absorption peak; the smaller the waveform first rule reference value is, the smoother the waveform corresponding to the absorption peak is, and the less likely the absorption peak is to be generated by noise; wherein, the method for obtaining the mean square error is a well-known technique and will not be described in detail. The reference band is divided into two local bands based on the wave number corresponding to the absorption peak as the dividing point; when the two local bands are more symmetrical, the absorption peak is more likely to be the target absorption peak corresponding to collagen, and then the absolute value of the difference in the area of ​​the two local bands corresponding to the infrared spectrum is obtained as the waveform second rule reference value of the absorption peak; the smaller the second rule reference value is, the more symmetrical the two local bands are, and the less likely the absorption peak is to be generated by noise; wherein, the area of ​​the area corresponding to the local band in the infrared spectrum can be directly obtained by existing methods, and will not be described in detail here. Then, the result of negatively correlating the product of the waveform first rule reference value and the waveform second rule reference value is used as the waveform regularity of the absorption peak; the greater the waveform regularity, the more likely the absorption peak is the target absorption peak corresponding to collagen;

[0084] Finally, the product of the prominence of the absorption peak and the regularity of the waveform is normalized to obtain the non-noise degree of the absorption peak.

[0085] The calculation formula of non-noise degree is: ; In the formula, is the non-noise degree of the a-th absorption peak; is the maximum absorbance in the cluster of the a-th absorption peak; is the length of the reference band of the ath absorption peak; is the prominence of the ath absorption peak; is the area of ​​the first local band of the a-th absorption peak in the infrared spectrum; is the area of ​​the second local band of the a-th absorption peak in the infrared spectrum; is the second rule reference value of the waveform of the ath absorption peak; is the first rule reference value of the waveform of the ath absorption peak; is the first preset constant, greater than 0; is the regularity of the waveform of the ath absorption peak; is the absolute value function; norm is the normalization function.

[0086] This embodiment will Set to 0.1 to avoid the denominator being 0. The implementer can set it according to the actual situation. The size is not limited here.

[0087] At this point, the noise-free degree of each absorption peak is obtained.

[0088] Step S203: when the non-noise level is greater than or equal to a preset non-noise level threshold, the corresponding absorption peak is used as a target absorption peak.

[0089] It is known that the greater the non-noise degree, the less likely the corresponding absorption peak is to be formed by noise, and the more likely it is to be the target absorption peak corresponding to collagen. Furthermore, this embodiment sets the preset non-noise degree threshold to 0.88. The implementer can set the size of the preset non-noise degree threshold according to actual conditions, and it is not limited here. When the non-noise degree is greater than or equal to the preset non-noise degree threshold, the corresponding absorption peak is used as the target absorption peak. At this point, the target absorption peak corresponding to collagen is accurately screened out, avoiding noise interference, which is conducive to the subsequent accurate analysis of the content of each type of collagen in the collagen to be detected.

[0090] Step S3: According to the position of the waveband corresponding to the target absorption peak, the type of collagen corresponding to each target absorption peak is obtained.

[0091] It is known that each type of collagen has multiple different target absorption peaks in the infrared spectrum. These target absorption peaks are related to the vibration of different chemical bonds in its molecules. Among them, the standard absorption wavenumbers corresponding to the NH bond, C=O bond and CH bond of type I collagen, type II collagen and type III collagen are the same. The standard absorption wavenumber corresponding to the NH bond is , the standard absorption wave number corresponding to the C=O bond is , the standard absorption wave number corresponding to the CH bond is ; The standard absorption wave number corresponding to the CN bond of type I collagen is , the standard absorption wavenumber corresponding to the CN bond of type II collagen and type III collagen is . The closer the waveband corresponding to the target absorption peak is to a certain standard absorption wavenumber, the more accurately the chemical bond corresponding to the target absorption peak can be determined, and the type of collagen corresponding to the target absorption peak can be determined based on the characteristics of the chemical bonds of different collagen types. Therefore, this embodiment obtains the type of collagen corresponding to each target absorption peak according to the position of the waveband corresponding to the target absorption peak. It should be noted that the target absorption peaks in the infrared spectrum are all absorption peaks corresponding to the chemical bonds NH bond, C=O bond, CH bond and CN bond.

[0092] Preferably, in a method that can be implemented in the present embodiment, the method for obtaining the collagen type corresponding to each target absorption peak is: first, according to the position distribution between each standard absorption wavenumber and the reference band of each target absorption peak, the adjacent target absorption peak of each standard absorption wavenumber is obtained, and the target absorption peak corresponding to the various collagen types corresponding to each chemical bond is indirectly determined, which is conducive to the subsequent accurate determination of the collagen type corresponding to each target absorption peak. Among them, the method for obtaining the adjacent target absorption peak is: for any standard absorption wavenumber and any target absorption peak, the left boundary wavenumber of the reference band of the target absorption peak is used as the first wavenumber of the target absorption peak; the right boundary wavenumber of the reference band of the target absorption peak is used as the second wavenumber of the target absorption peak; the absolute value of the difference between the first wavenumber and the standard absorption wavenumber is used as the first difference; the absolute value of the difference between the second wavenumber and the standard absorption wavenumber is used as the second difference; when the first difference and the second difference are both smaller, it means that the target absorption peak is closer to the standard absorption wavenumber, and then the product of the first difference and the second difference is normalized as the result of the position approximation between the target absorption peak and the standard absorption wavenumber; it should be noted that, in this embodiment, the product of the first difference and the second difference is normalized by the norm normalization function. Among them, the smaller the position approximation, the more likely the target absorption peak is to be the adjacent target absorption peak of the standard absorption wave number, and thus the preset position approximation threshold is set to 0.45 in this embodiment. The implementer can set the value of the preset position approximation threshold according to the actual situation, which is not limited here. When the position approximation is less than the preset position approximation threshold, the corresponding target absorption peak is used as the adjacent target absorption peak of the standard absorption wave number. At this point, the adjacent target absorption peak of each standard absorption wave number is obtained;

[0093] It is known that the standard absorption wavenumbers corresponding to the NH bonds and C=O bonds of type I collagen, type II collagen, and type III collagen are the same. At the same time, for NH bonds and C=O bonds, the differences between the peak intensities of the target absorption peaks corresponding to different collagen types are quite obvious. Among them, the peak intensity of the target absorption peak corresponding to the NH bonds and C=O bonds of type I collagen is the largest, the peak intensity of the target absorption peak corresponding to the NH bonds and C=O bonds of type II collagen is smaller than that of type I collagen and greater than that of type III collagen, and the peak intensity of the target absorption peak corresponding to the NH bonds and C=O bonds of type III collagen is the smallest. Furthermore, in this embodiment, the standard absorption wavenumbers corresponding to the NH bond and the C=O bond are both used as the first standard absorption wavenumber. For any first standard absorption wavenumber, the first preset number of target absorption peaks whose corresponding wavenumbers among the neighboring target absorption peaks of the first standard absorption wavenumber are closest to the first standard absorption wavenumber are used as reference absorption peaks of the first standard absorption wavenumber; wherein, when the total number of the neighboring target absorption peaks of the first standard absorption wavenumber is greater than or equal to the number of types of collagen, i.e., 3, the first preset number is the number of types of collagen; when the total number of the neighboring target absorption peaks of the first standard absorption wavenumber is less than the number of types of collagen, the first preset number is the total number of the neighboring target absorption peaks of the standard absorption wavenumber;

[0094] Then, according to the difference in absorbance between each reference absorption peak of the first standard absorption wavenumber and other reference absorption peaks, the type of collagen corresponding to each reference absorption peak of the first standard absorption wavenumber is obtained; the specific process is: for any reference absorption peak of the first standard absorption wavenumber, the difference in absorbance between the reference absorption peak and each other reference absorption peak of the first standard absorption wavenumber is obtained, and each is used as the reference difference value of the reference absorption peak; the accumulated result of the reference difference values ​​of the reference absorption peak is used as the peak intensity of the reference absorption peak; the peak intensity of each reference absorption peak of the first standard absorption wavenumber is obtained, and when the number of reference absorption peaks of the first standard absorption wavenumber is equal to the number of collagen types, that is, 3, the collagen type corresponding to the reference absorption peak corresponding to the maximum peak intensity is set to type I; The collagen type corresponding to the reference absorption peak corresponding to the second largest peak intensity is set as type II; the collagen type corresponding to the reference absorption peak corresponding to the smallest peak intensity is set as type III; when the number of reference absorption peaks of the first standard absorption wavenumber is less than the number of collagen types, and the number of reference absorption peaks of the first standard absorption wavenumber is 2, the collagen type corresponding to the reference absorption peak corresponding to the largest peak intensity is set as type I; the collagen type corresponding to the reference absorption peak corresponding to the smallest peak intensity is set as type II; when the number of reference absorption peaks of the first standard absorption wavenumber is less than the number of collagen types, and the number of reference absorption peaks of the first standard absorption wavenumber is 1, the collagen type corresponding to the reference absorption peak of the first standard absorption wavenumber is set as type I;

[0095] It is known that the standard absorption wavenumbers corresponding to the CH bonds of type I collagen, type II collagen, and type III collagen are the same. For the CH bonds, the difference between the peak intensities of the target absorption peaks corresponding to different collagen types is not obvious, but there are obvious differences in the lengths of the reference bands of the target absorption peaks corresponding to different collagen types. Among them, the length of the reference band of the target absorption peak corresponding to the CH bonds of type II collagen is the largest, the length of the reference band of the target absorption peak corresponding to the CH bonds of type I collagen is smaller than that of type II collagen and larger than that of type III collagen, and the length of the reference band of the target absorption peak corresponding to the CH bonds of type III collagen is the smallest. For better description, this embodiment uses the standard absorption wavenumber corresponding to the CH bond as the second standard absorption wavenumber, and then obtains the collagen type corresponding to each reference absorption peak of the second standard absorption wavenumber according to the length of the reference band of each reference absorption peak of the second standard absorption wavenumber; the specific process is: the length of the reference band of each reference absorption peak of the second standard absorption wavenumber is used as the first length; when the number of reference absorption peaks of the second standard absorption wavenumber is equal to the number of types of collagen, that is, 3, the collagen type corresponding to the reference absorption peak corresponding to the largest first length is set to type II; the collagen type corresponding to the reference absorption peak corresponding to the second largest first length is set to type I; the collagen type corresponding to the reference absorption peak corresponding to the smallest first length is set to type III; when the second standard absorption When the number of reference absorption peaks of the absorption wavenumber is less than the number of collagen types and the number of reference absorption peaks of the second standard absorption wavenumber is 2, the collagen type corresponding to the reference absorption peak corresponding to the largest first length is set to type II; the collagen type corresponding to the reference absorption peak corresponding to the smallest first length is set to type I; when the number of reference absorption peaks of the second standard absorption wavenumber is less than the number of collagen types and the number of reference absorption peaks of the second standard absorption wavenumber is 1, the collagen type corresponding to the reference absorption peak of the second standard absorption wavenumber is set to type I, because the first length of the reference absorption peak of the second standard absorption wavenumber is not considered at this time, and usually type I collagen is the most common, and the wavenumber of the target absorption peak corresponding to type I collagen is usually the closest to the second standard absorption wavenumber;

[0096] It is known that the standard absorption wavenumbers corresponding to the CN bonds of type II collagen and type III collagen are , the standard absorption wave number corresponding to the CN bond of type I collagen is For CN bonds, the differences in the peak intensities of the target absorption peaks corresponding to different collagen types are quite obvious. Among them, the peak intensity of the target absorption peak corresponding to the CN bonds of type I collagen is the largest, the peak intensity of the target absorption peak corresponding to the CN bonds of type II collagen is smaller than that of type I collagen and larger than that of type III collagen, and the peak intensity of the target absorption peak corresponding to the CN bonds of type III collagen is the smallest. For ease of description, this embodiment uses the standard absorption wavenumber corresponding to the CN bond of type I collagen as the third standard absorption wavenumber, and the standard absorption wavenumber corresponding to the CN bond of type II collagen and type III collagen as the fourth standard absorption wavenumber; the collagen type corresponding to the target absorption peak whose corresponding wavenumber is closest to the third standard absorption wavenumber among the adjacent target absorption peaks of the third standard absorption wavenumber is set to type I; the two target absorption peaks whose corresponding wavenumbers are closest to the fourth standard absorption wavenumber among the adjacent target absorption peaks of the fourth standard absorption wavenumber are used as matching absorption peaks of the fourth standard absorption wavenumber; the absorbance of each matching absorption peak is obtained as the first eigenvalue of the corresponding matching absorption peak; the collagen type corresponding to the matching absorption peak corresponding to the largest first eigenvalue is set to type II; the collagen type corresponding to the matching absorption peak corresponding to the smallest first eigenvalue is set to type III; when there is one adjacent target absorption peak of the fourth standard absorption wavenumber, the collagen type corresponding to the adjacent target absorption peak of the fourth standard absorption wavenumber is set to type II.

[0097] At this point, the type of collagen corresponding to each target absorption peak in the infrared spectrum is determined.

[0098] Step S4: Obtain the content of each collagen type according to the area of ​​all target absorption peaks corresponding to each collagen type.

[0099] It is known that the area of ​​the target absorption peak in the infrared spectrum reflects the content of the corresponding collagen type. In order to determine the content of each collagen type in the collagen to be detected, this embodiment obtains the content of each collagen type based on the area of ​​all target absorption peaks corresponding to each collagen type. Among them, for any target absorption peak, the area of ​​the region corresponding to the reference band of the target absorption peak in the infrared spectrum image is used as the area of ​​the target absorption peak. It should be noted that the area of ​​the region corresponding to the reference band of the target absorption peak in the infrared spectrum image can be directly obtained by the current method, which will not be repeated here. At this point, the area of ​​each target absorption peak is obtained.

[0100] Preferably, in a method that can be implemented in this embodiment, the method for obtaining the content of each collagen type is: for any collagen type, the area accumulation of all target absorption peaks corresponding to the collagen type is normalized and the result is used as the content of the collagen type. It should be noted that in this embodiment, the result of the area accumulation of all target absorption peaks corresponding to the collagen type is normalized by the norm normalization function.

[0101] At this point, the content of each collagen type is obtained.

[0102] Step S5: Determine the function of the collagen to be detected based on the content of each collagen type.

[0103] Based on the collagen type of the collagen to be tested and the content of each collagen type, the specific function of the collagen to be tested can be determined. Since the function of each collagen type is known, the role of the collagen to be tested in different diseases can be accurately studied, which is helpful for early diagnosis of the disease and monitoring of the progression of the disease.

[0104] In summary, this embodiment obtains an infrared spectrum of the collagen to be detected; based on the distribution of absorption peaks in the infrared spectrum, the target absorption peaks generated by non-noise are screened out; according to the position of the band corresponding to the target absorption peak, the type of collagen corresponding to each target absorption peak is obtained; according to the area of ​​all target absorption peaks corresponding to each collagen type, the content of each collagen type is obtained; based on the content of each collagen type, the function of the collagen to be detected is determined. The present invention avoids the confusion of collagen types by accurately determining the collagen type corresponding to the target absorption peak, so that the content of each collagen type can be accurately obtained, and then the function of the collagen to be detected can be accurately analyzed.

[0105] Embodiment 2:

[0106] The present invention also proposes a collagen detection system, please refer to Figure 3 , which shows a structural diagram of a collagen detection system provided by an embodiment of the present invention, the system includes: an acquisition module 10, a target absorption peak acquisition module 20, a collagen type acquisition module 30, a collagen type content acquisition module 40 and a function detection module 50.

[0107] The acquisition module 10 is used to acquire the infrared spectrum of the collagen to be detected.

[0108] The target absorption peak acquisition module 20 is used to screen out the target absorption peak that is not generated by noise based on the distribution of the absorption peaks in the infrared spectrum.

[0109] The collagen type acquisition module 30 is used to acquire the collagen type corresponding to each target absorption peak according to the position of the waveband corresponding to the target absorption peak.

[0110] The collagen type content acquisition module 40 is used to acquire the content of each collagen type according to the area of ​​all target absorption peaks corresponding to each collagen type.

[0111] The function detection module 50 is used to determine the function of the collagen to be detected based on the content of each type of collagen.

[0112] It should be noted that the system provided in the above embodiment is only illustrated by the division of the above functional modules. In actual applications, the above functions can be assigned to different functional modules as needed, that is, the internal structure of the computer device is divided into different functional modules to complete all or part of the functions described above. In addition, the collagen detection system and the collagen detection method embodiment provided in the above embodiment belong to the same concept. The specific implementation process is detailed in the method embodiment and will not be repeated here.

[0113] Embodiment 3:

[0114] The present invention also proposes a collagen detection device, which includes a memory and a processor, wherein the memory stores an executable program code, and the processor is used to call and execute the executable program code to execute a collagen detection method provided in an embodiment of the present application. The device can be a chip, a component or a module, and the chip may include a connected processor and a memory; wherein the memory is used to store instructions, and when the processor calls and executes the instructions, the chip can execute a collagen detection method provided in the above embodiment.

[0115] In addition, the present application embodiment also protects a computer device, see Figure 4 The computer device includes a memory 401, a processor 402, and a computer program 403 stored in the memory 401 and running on the processor 402, wherein when the processor 402 executes the computer program 403, the computer device can execute any one of the collagen detection methods introduced above.

[0116] Embodiment 4:

[0117] This embodiment also provides a computer-readable storage medium, in which a computer program code is stored. When the computer program code is run on a computer, the computer executes the above-mentioned related method steps to implement a collagen detection method provided in the above embodiment.

[0118] Embodiment 5:

[0119] This embodiment also provides a computer program product. When the computer program product is run on a computer, the computer is enabled to execute the above-mentioned related steps to implement a collagen detection method provided in the above embodiment.

[0120] Among them, the device, computer-readable storage medium, computer program product or chip provided in this embodiment is used to execute the corresponding method provided above. Therefore, the beneficial effects that can be achieved can refer to the beneficial effects in the corresponding method provided above, and will not be repeated here.

[0121] It should be noted that the sequence of the above embodiments of the present invention is only for description and does not represent the advantages and disadvantages of the embodiments. The processes depicted in the accompanying drawings do not necessarily require the specific order or continuous order shown to achieve the desired results. In some embodiments, multitasking and parallel processing are also possible or may be advantageous.

[0122] The various embodiments in this specification are described in a progressive manner, and the same or similar parts between the various embodiments can be referenced to each other, and each embodiment focuses on the differences from other embodiments.

Claims

1. A method for detecting collagen, characterized in that: The method comprises the following steps: Obtaining an infrared spectrum of the collagen to be detected; Filter out the target absorption peaks not generated by noise based on the distribution of absorption peaks in the infrared spectrum; According to the position of the waveband corresponding to the target absorption peak, the type of collagen corresponding to each target absorption peak is obtained; wherein the collagen types include type I, type II and type III; According to the area of ​​all target absorption peaks corresponding to each collagen type, the content of each collagen type is obtained; Determining the function of the collagen to be tested based on the amount of each collagen type; The method for obtaining the target absorption peak is: The absorbance corresponding to each wave number in the infrared spectrum is clustered by the ISODATA clustering algorithm to obtain the clustering cluster of each absorption peak in the infrared spectrum; According to the distribution of the absorbance in the cluster of each absorption peak and the wave number corresponding to the absorbance, the non-noise degree of each absorption peak is obtained; When the non-noise level is greater than or equal to a preset non-noise level threshold, the corresponding absorption peak is used as the target absorption peak; The method for obtaining the non-noise degree is: For any absorption peak, the waveband consisting of the wavenumber corresponding to the absorbance in the cluster of the absorption peak is used as the reference waveband of the absorption peak; The ratio of the maximum absorbance in the cluster of the absorption peak to the length of the reference band is taken as the prominence of the absorption peak; Obtaining the mean square error of all absorbances in the cluster of the absorption peak as the first rule reference value of the waveform of the absorption peak; The reference band is divided using the wave number corresponding to the absorption peak as a dividing point to obtain two local bands; Obtaining the difference in area between the two local bands corresponding to the infrared spectrum as a second rule reference value of the waveform of the absorption peak; The result of negatively correlating the product of the first waveform rule reference value and the second waveform rule reference value is used as the waveform rule degree of the absorption peak; The result of normalizing the product of the prominence of the absorption peak and the regularity of the waveform is taken as the non-noise degree of the absorption peak.

2. A collagen detection method according to claim 1, characterized in that: The method for obtaining the type of collagen corresponding to each target absorption peak according to the position of the waveband corresponding to the target absorption peak is: Obtain the standard absorption wavenumber corresponding to each chemical bond of each collagen type; wherein the chemical bonds include NH bonds, C=O bonds, CH bonds and CN bonds; According to the position distribution between each standard absorption wave number and the reference band of each target absorption peak, the adjacent target absorption peak of each standard absorption wave number is obtained; The standard absorption wavenumbers corresponding to the NH bond and the C=O bond of each collagen type are the same, and the standard absorption wavenumbers corresponding to the NH bond and the C=O bond are all used as the first standard absorption wavenumber. For any first standard absorption wavenumber, the first preset number of target absorption peaks whose corresponding wavenumbers are closest to the first standard absorption wavenumber among the adjacent target absorption peaks of the first standard absorption wavenumber are used as reference absorption peaks of the first standard absorption wavenumber; Wherein, when the total number of adjacent target absorption peaks of the first standard absorption wavenumber is greater than or equal to the number of types of collagen, the first preset number is the number of types of collagen; when the total number of adjacent target absorption peaks of the first standard absorption wavenumber is less than the number of types of collagen, the first preset number is the total number of adjacent target absorption peaks of the standard absorption wavenumber; Obtaining the type of collagen corresponding to each reference absorption peak of the first standard absorption wavenumber according to the difference in absorbance between each reference absorption peak of the first standard absorption wavenumber and other reference absorption peaks; The standard absorption wavenumber corresponding to the CH bond of each collagen type is the same, the standard absorption wavenumber corresponding to the CH bond is used as the second standard absorption wavenumber, and the collagen type corresponding to each reference absorption peak of the second standard absorption wavenumber is obtained according to the length of the reference band of each reference absorption peak of the second standard absorption wavenumber; The standard absorption wavenumbers corresponding to the CN bonds of type II collagen and type III collagen are the same, and the standard absorption wavenumbers corresponding to the CN bonds of type I collagen and type II collagen are different. The standard absorption wavenumber corresponding to the CN bond of type I collagen is used as the third standard absorption wavenumber, and the standard absorption wavenumber corresponding to the CN bond of type II collagen and type III collagen is used as the fourth standard absorption wavenumber; The collagen type corresponding to the target absorption peak whose wave number is closest to the third standard absorption wave number among the target absorption peaks adjacent to the third standard absorption wave number is set as type I; The two target absorption peaks whose corresponding wavenumbers are closest to the fourth standard absorption wavenumber among the target absorption peaks adjacent to the fourth standard absorption wavenumber are used as matching absorption peaks of the fourth standard absorption wavenumber; Obtaining the absorbance of each matching absorption peak as the first characteristic value of the corresponding matching absorption peak; The collagen type corresponding to the matching absorption peak corresponding to the largest first eigenvalue is set as type II; The collagen type corresponding to the matching absorption peak corresponding to the smallest first eigenvalue is set as type III; When there is one adjacent target absorption peak of the fourth standard absorption wave number, the collagen type corresponding to the adjacent target absorption peak of the fourth standard absorption wave number is set to type II.

3. A collagen detection method as claimed in claim 2, characterized in that: The method for obtaining the adjacent target absorption peak is: For any standard absorption wave number and any target absorption peak, the left boundary wave number of the reference band of the target absorption peak is taken as the first wave number of the target absorption peak; The right boundary wave number of the reference band of the target absorption peak is used as the second wave number of the target absorption peak; The difference between the first wave number and the standard absorption wave number is taken as the first difference; The difference between the second wave number and the standard absorption wave number is taken as the second difference; Normalizing the product of the first difference and the second difference as the approximation degree between the positions of the target absorption peak and the standard absorption wave number; When the position approximation is less than a preset position approximation threshold, the corresponding target absorption peak is used as the adjacent target absorption peak of the standard absorption wave number.

4. A collagen detection method as claimed in claim 2, characterized in that: The method for obtaining the type of collagen corresponding to each reference absorption peak of the first standard absorption wavenumber according to the difference in absorbance between each reference absorption peak of the first standard absorption wavenumber and other reference absorption peaks is: For any reference absorption peak of the first standard absorption wavenumber, obtaining the difference between the absorbance of the reference absorption peak and each other reference absorption peak of the first standard absorption wavenumber, and taking them as the reference difference value of the reference absorption peak; The accumulated result of the reference difference values ​​of the reference absorption peak is used as the peak intensity of the reference absorption peak; Obtaining the peak intensity of each reference absorption peak of the first standard absorption wavenumber, when the number of reference absorption peaks of the first standard absorption wavenumber is equal to the number of collagen types, setting the collagen type corresponding to the reference absorption peak corresponding to the largest peak intensity as type I; setting the collagen type corresponding to the reference absorption peak corresponding to the second largest peak intensity as type II; setting the collagen type corresponding to the reference absorption peak corresponding to the smallest peak intensity as type III; When the number of reference absorption peaks of the first standard absorption wavenumber is less than the number of types of collagen, and the number of reference absorption peaks of the first standard absorption wavenumber is 2, the collagen type corresponding to the reference absorption peak corresponding to the maximum peak intensity is set to type I; the collagen type corresponding to the reference absorption peak corresponding to the minimum peak intensity is set to type II; When the number of reference absorption peaks of the first standard absorption wavenumber is less than the number of collagen types and the number of reference absorption peaks of the first standard absorption wavenumber is 1, the collagen type corresponding to the reference absorption peak of the first standard absorption wavenumber is set to type I.

5. A collagen detection method as claimed in claim 2, characterized in that: The method for obtaining the type of collagen corresponding to each reference absorption peak of the second standard absorption wavenumber according to the size of the reference waveband of each reference absorption peak of the second standard absorption wavenumber is: The length of the reference waveband of each reference absorption peak of the second standard absorption wavenumber is taken as the first length; When the number of reference absorption peaks of the second standard absorption wavenumber is equal to the number of collagen types, the collagen type corresponding to the reference absorption peak corresponding to the largest first length is set to type II; the collagen type corresponding to the reference absorption peak corresponding to the second largest first length is set to type I; the collagen type corresponding to the reference absorption peak corresponding to the smallest first length is set to type III; When the number of reference absorption peaks of the second standard absorption wavenumber is less than the number of types of collagen, and the number of reference absorption peaks of the second standard absorption wavenumber is 2, the collagen type corresponding to the reference absorption peak corresponding to the largest first length is set to type II; the collagen type corresponding to the reference absorption peak corresponding to the smallest first length is set to type I; When the number of reference absorption peaks of the second standard absorption wavenumber is less than the number of collagen types and the number of reference absorption peaks of the second standard absorption wavenumber is 1, the collagen type corresponding to the reference absorption peak of the second standard absorption wavenumber is set to type I.

6. A collagen detection method according to claim 1, characterized in that: The method for obtaining the area of ​​the target absorption peak is: For any target absorption peak, the area of ​​the region corresponding to the reference band of the target absorption peak in the infrared spectrum image is taken as the area of ​​the target absorption peak.

7. A collagen detection method according to claim 1, characterized in that: The method for obtaining the content of each collagen type is: For any type of collagen, the area accumulation of all target absorption peaks corresponding to the type of collagen is normalized and the result is taken as the content of the type of collagen.

8. A collagen detection device, comprising a memory, a processor, and a computer program stored in the memory and running on the processor, characterized in that: When executing the computer program, the processor implements the steps of a collagen detection method as described in any one of claims 1 to 7.

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