A bio-based anti-adhesion hydrogel dressing and its preparation method and application

Abstract

The present invention discloses a bio-based anti-adhesion hydrogel dressing and a preparation method and application thereof, which belongs to the technical field of biomedical materials, and provides a preparation method of a bio-based anti-adhesion hydrogel dressing, including: mixing a bio-based monomer and water to obtain a bio-based monomer aqueous solution; mixing with a cross-linking agent to react to obtain a bio-based monomer aqueous solution after reaction; mixing a natural organic compound, anhydrous ethanol and a polyol to obtain a mixed solution; combining the mixed solution with the bio-based monomer aqueous solution after reaction to obtain a complete reaction solution; adding a silicone oil mixture to freeze to obtain a hydrogel dressing; the natural organic compound is one or more of tannic acid, proanthocyanidin or gallic acid. The present invention prepares a skin-like hydrogel dressing by mixing and preparing raw materials in a one-pot method, which can be applied to a wound to fit and achieve the effect of preventing blood adhesion.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a bio-based anti-adhesion hydrogel dressing and a preparation method and application thereof. Background Art

[0002] At present, chronic wound infection has become a very difficult biomedical problem, and wound infection often produces several serious adverse symptoms. Wound dressings can act as a skin barrier to protect wounds, prevent wound infection, absorb wound exudate, and promote the healing of chronic wounds.

[0003] Hydrogel dressing is a new type of wound dressing with good comprehensive hydrophilic properties, excellent antibacterial properties and wound healing performance. Hydrogels are usually three-dimensional cross-linked network structures. According to the different forms of cross-linking, they can be divided into chemical hydrogels and physical hydrogels, and the performance can be adjusted as needed. Hydrogel dressings have a high water content, which can accelerate wound cleaning and promote wound healing in a moist environment. At the same time, they act as a natural barrier to protect wounds, isolate the invasion of microorganisms and bacteria, and accelerate cell regeneration, proliferation, and the death and decomposition of damaged cells. However, common hydrogel dressings on the market often have mechanical defects and are difficult to detach, which makes it easy for patients to cause secondary damage to their wounds when changing dressings. Self-peeling hydrogel dressings have typical sliding properties, which can eliminate the adhesion of the wound under certain conditions, avoid adhesion, and reduce the damage caused by changing dressings and the impact on wound healing. Therefore, self-peeling / shedding hydrogel dressings have become a research hotspot in the field of hydrogel dressings.

[0004] Excessive blood loss during the formation of blood clots, the dressing used cannot cover the wound, resulting in the inability to form a solid clot to stop the bleeding process. The firm adhesion of the blood clot on the hemostatic dressing causes the blood clot to be torn when the wound dressing is removed, resulting in pain, secondary bleeding and infection. Some researchers have proposed the preparation of superhydrophilic dressings to absorb blood to form a solid clot-dressing complex, and prepared porous polyvinyl alcohol (PVA) / alginate composite bubbles to promote thrombosis, blood coagulation, and the ability of blood cell adhesion and aggregation; in order to solve the problem of secondary bleeding caused by strong adhesion, researchers proposed the preparation of superhydrophobic dressings, imitating the surface structure of lotus leaves, and modifying polydopamine, perfluorocarbon and silver nanoparticles on ordinary medical gauze. However, their materials cannot be compatible with overcoming the above two challenges at the same time, and will cause defects on the other side when achieving the performance on one side. Therefore, the synergistic effect of the two functions of hemostasis and anti-adhesion is a direction worth studying for medical dressings. Summary of the invention

[0005] In order to solve the above technical problems, the present invention proposes a bio-based anti-adhesion hydrogel dressing and a preparation method and application thereof. The present invention prepares a skin-like hydrogel dressing by mixing raw materials in a one-pot method, which can be applied to the wound to fit and achieve the effect of preventing blood adhesion.

[0006] To achieve the above object, the present invention provides a method for preparing a bio-based anti-adhesion hydrogel dressing, comprising the following steps:

[0007] (1) mixing the bio-based monomer and water to obtain a bio-based monomer aqueous solution;

[0008] (2) mixing the bio-based monomer aqueous solution obtained in step (1) with a cross-linking agent, and reacting the mixture to obtain a reacted bio-based monomer aqueous solution;

[0009] (3) Mixing the natural organic compound, anhydrous ethanol and polyol, and stirring until clear to obtain a mixed solution;

[0010] (4) mixing the aqueous solution of the bio-based monomer obtained after the reaction in step (2) and the mixed solution obtained in step (3), stirring and reacting to obtain a completely reacted solution;

[0011] (5) adding the silicone oil mixture to the reaction complete solution obtained in step (4), freezing, and obtaining a hydrogel dressing;

[0012] The natural organic compound in step (3) is one or more of tannic acid, proanthocyanidin or gallic acid.

[0013] Preferably, the bio-based monomer in step (1) is one or more of gelatin, chitosan, carboxymethyl chitosan, agar or agarose; the temperature of the water in step (1) is 40-60° C.; the mass ratio of the bio-based monomer to water in step (1) is 1-5:10-20.

[0014] Preferably, the mixing ratio of the bio-based monomer aqueous solution and the cross-linking agent in step (2) is calculated according to the mass ratio of the bio-based monomer to the cross-linking agent of 1-5:0.01-1; the cross-linking agent in step (2) is a biological cross-linking agent or a chemical cross-linking agent, the biological cross-linking agent is transglutaminase, the enzyme activity of the transglutaminase is 100-500 U / g, and the chemical cross-linking agent is genipin and / or glutaraldehyde.

[0015] Preferably, the reaction time in step (2) is 1 to 10 hours.

[0016] Preferably, the mixing ratio of the natural organic compound, anhydrous ethanol and polyol in step (3) is calculated according to the mass ratio of bio-based monomer, natural organic compound, anhydrous ethanol and polyol of 1-5:0.1-1:5-10:1-5; the polyol in step (3) is one or more of glycerol, propylene glycol or polyethylene glycol.

[0017] Preferably, the stirring reaction time in step (4) is 1 to 2 hours.

[0018] Preferably, the silicone oil mixture in step (5) is one or more of dimethyl silicone oil, hydroxy silicone oil, vinyl silicone oil, phenyl silicone oil, amino silicone oil or bisamino silicone oil; the silicone oil mixture in step (5) is a mixture of two of dimethyl silicone oil, hydroxy silicone oil, vinyl silicone oil, phenyl silicone oil, amino silicone oil or bisamino silicone oil.

[0019] Preferably, the freezing temperature in step (5) is -20 to -50°C, and the freezing time is 0.5 to 48 hours.

[0020] The invention also provides the hydrogel dressing prepared by the preparation method.

[0021] The present invention also provides the hydrogel dressing prepared by the preparation method or the application of the hydrogel dressing in the preparation of biomedical hemostatic materials.

[0022] Compared with the prior art, the present invention has the following advantages and technical effects:

[0023] The present invention prepares a bio-based anti-adhesion skin hydrogel dressing, which can be applied to wounds and achieve the effect of preventing blood adhesion. Different molds can be used to prepare hydrogel dressings of different thicknesses and sizes, and the ratios in the test process are consistent, and the performance of the hydrogel dressing remains unchanged; it can be instantly contacted and adhered to different substrates such as iron, aluminum, PP, glass and PET, and can fit tightly to the skin, showing the performance of skin-like hydrogel in the same color as the skin; it remains soft and does not change color after being placed in the air for five days; it has good blood adhesion prevention performance; after being soaked in water for 1 hour, the hydrogel dressing of the present invention will only absorb water and swell, but will not break, and has good underwater stability.

[0024] Compared with existing technologies, completely bio-based raw materials are used for simplified production, and at the same time, the two pain points of excessive blood loss during the formation of blood clots in traditional dressings and the firm adhesion of blood clots on hemostatic dressings are solved. This effect can be achieved because the interface of the hydrogel dressing has both adhesion and anti-adhesion properties. Adhesion means that it can adhere to the substrate, and anti-adhesion means that when it comes into contact with blood, the blood can easily form a blood clot without adhering to the blood clot. This is an effect that previous technologies and solutions did not have and is also a core key feature. The use of gelatin monomers to prepare a hydrogel with good biocompatibility is combined with the hydrogen bond adhesion effect provided by tannic acid and glycerol and the anti-adhesion effect provided by dimethyl silicone oil and amino silicone oil, and a synergistic effect is achieved between the two, thereby solving the two major contradictions of current traditional dressings. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 The instantaneous adhesion test of the hydrogel dressing of Example 1 and different substrates is shown in FIG. 1 , wherein A is iron, B is aluminum, C is PP, D is glass, E is PET, and F is skin;

[0027] Figure 2 is the contact angle between the upper surface of the hydrogel dressing of Example 1 and water / blood, wherein A is the contact angle between the upper surface and water, and B is the contact angle between the upper surface and blood;

[0028] Figure 3 is the contact angle between the lower surface of the hydrogel dressing of Example 1 and water / blood, wherein A is the contact angle between the lower surface and water, B is the contact angle between the lower surface and blood, C is the contact angle between the lower surface and water, and D is the sliding angle between the lower surface and water;

[0029] Figure 4 The tensile properties of the hydrogel dressing of Example 1 are measured, wherein A is the original state of the hydrogel dressing of Example 1, and B is the state of the hydrogel dressing of Example 1 after stretching;

[0030] Figure 5 The softness test of the hydrogel dressing of Example 1, wherein A is folded in half and B is folded diagonally;

[0031] Figure 6 The air stability test of the hydrogel dressing of Example 1 is as follows;

[0032] Figure 7The blood adhesion performance of the hydrogel dressing of Example 1 is measured, wherein A is the hydrogel dressing dripped with blood, and B is the blood coagulated and separated from the hydrogel dressing;

[0033] Figure 8 The underwater stability of the hydrogel dressing of Example 1, wherein A is the hydrogel dressing immersed in water, and B is the hydrogel dressing after being immersed for 1 hour;

[0034] Fig. 9 The instantaneous adhesion test of the hydrogel dressing of Example 2 and different substrates, wherein A is iron, B is aluminum, C is PP, D is glass, E is PET, and F is skin;

[0035] Fig.10 is the contact angle between the upper surface of the hydrogel dressing of Example 2 and water / blood, wherein A is the contact angle between the upper surface and water, and B is the contact angle between the upper surface and blood;

[0036] Fig.11 is the contact angle between the lower surface of the hydrogel dressing of Example 2 and water / blood, wherein A is the contact angle between the lower surface and water, B is the contact angle between the lower surface and blood, C is the contact angle between the lower surface and water, and D is the sliding angle between the lower surface and water;

[0037] Fig.12 The tensile properties of the hydrogel dressing of Example 2 are measured, wherein A is the original state of the hydrogel dressing of Example 2, and B is the state of the hydrogel dressing of Example 2 after stretching;

[0038] Fig.13 The softness test of the hydrogel dressing of Example 2, wherein A is folded in half and B is folded diagonally;

[0039] Fig.14 The blood adhesion performance of the hydrogel dressing of Example 2 is measured, wherein A is the hydrogel dressing dripped with blood, and B is the blood coagulated and separated from the hydrogel dressing;

[0040] Fig.15 The underwater stability of the hydrogel dressing of Example 2, wherein A is the hydrogel dressing immersed in water, and B is the hydrogel dressing after being immersed for 1 hour;

[0041] Fig.16 The instantaneous adhesion test of the hydrogel dressing of Example 3 to different substrates, wherein A is iron, B is aluminum, C is PP, D is glass, E is PET, and F is skin;

[0042] Fig.17 is the contact angle between the upper surface of the hydrogel dressing of Example 3 and water / blood, wherein A is the contact angle between the upper surface and water, and B is the contact angle between the upper surface and blood;

[0043] Fig.18is the contact angle between the lower surface of the hydrogel dressing of Example 3 and water / blood, wherein A is the contact angle between the lower surface and water, B is the contact angle between the lower surface and blood, C is the contact angle between the lower surface and water, and D is the sliding angle between the lower surface and water;

[0044] Fig.19 The tensile properties of the hydrogel dressing of Example 3 are measured, wherein A is the original state of the hydrogel dressing of Example 3, and B is the state of the hydrogel dressing of Example 3 after stretching;

[0045] Fig. 20 The softness test of the hydrogel dressing of Example 3, wherein A is folded in half and B is folded diagonally;

[0046] Fig.21 The blood adhesion performance of the hydrogel dressing of Example 3 is measured, wherein A is the hydrogel dressing dripped with blood, and B is the blood coagulated and separated from the hydrogel dressing;

[0047] Fig. 22 The underwater stability of the hydrogel dressing of Example 3, wherein A is the hydrogel dressing immersed in water, and B is the hydrogel dressing after being immersed for 1 hour. DETAILED DESCRIPTION

[0048] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0049] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0050] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0051] Without departing from the scope or spirit of the present invention, various modifications and variations may be made to the specific embodiments of the present invention description, which will be apparent to those skilled in the art. Other embodiments obtained from the present invention description will be apparent to those skilled in the art. The present invention description and examples are merely exemplary. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available commodities.

[0052] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0053] Example 1

[0054] (1) Mix 2 g of gelatin and 10 g of water at 55°C to obtain a gelatin aqueous solution;

[0055] (2) The gelatin aqueous solution was mixed with 0.1 g of glutamine transferase (enzyme activity of 100 U / g) and reacted for 1 h to obtain a gelatin aqueous solution after reaction;

[0056] (3) Mix 1 g of tannic acid, 5 g of anhydrous ethanol and 2 g of glycerol and stir until clear to obtain a mixed solution;

[0057] (4) After the reaction, the gelatin aqueous solution and the mixed solution are mixed and stirred for 1 hour to obtain a complete reaction solution;

[0058] (5) A silicone oil mixture (prepared by mixing 9 g of dimethyl silicone oil and 2 g of amino silicone oil) was added to the completely reacted solution, inverted in a 10 cm*10 cm*1 mm polytetrafluoroethylene mold, and frozen at -20°C for 24 h to obtain a hydrogel dressing.

[0059] The hydrogel dressing is thawed in an oven at 30°C. During this process, the hydrogel dressing can achieve orientation distribution and a sticky-slip synergistic effect at the interface in contact with the polytetrafluoroethylene mold, thereby achieving the effect of painlessly removing blood scabs while fitting and covering the wound.

[0060] Example 2

[0061] (1) Mix 3 g of gelatin and 12 g of water at 60°C to obtain a gelatin aqueous solution;

[0062] (2) The gelatin aqueous solution was mixed with 1 g of genipin and reacted for 1 h to obtain a gelatin aqueous solution after reaction;

[0063] (3) 0.2 g of proanthocyanidins, 6 g of anhydrous ethanol and 3 g of glycerol were mixed and stirred until clear to obtain a mixed solution;

[0064] (4) After the reaction, the gelatin aqueous solution and the mixed solution are mixed and stirred for 1 hour to obtain a complete reaction solution;

[0065] (5) A silicone oil mixture (prepared by mixing 9 g of hydroxy silicone oil and 3 g of amino silicone oil) was added to the complete reaction solution, inverted in a 10 cm*10 cm*1 mm polytetrafluoroethylene mold, and frozen at -20°C for 24 h to obtain a hydrogel dressing.

[0066] The hydrogel dressing was thawed in an oven at 30°C. During this process, the hydrogel dressing can achieve orientation distribution and achieve sticky-slip synergy at the interface in contact with the polytetrafluoroethylene mold, so that it can cover the wound and achieve the effect of painlessly removing the blood scab. The hydrogel dressing obtained by the reaction with genipin is black, which is different from the skin color.

[0067] Example 3

[0068] (1) Mix 2 g of agar with 12 g of water at 60°C to obtain an agar aqueous solution.

[0069] (2) The agar aqueous solution was mixed with 0.2 g of glutamine transferase (enzyme activity of 100 U / g) and reacted for 1 h to obtain the agar aqueous solution after the reaction;

[0070] (3) Mix 0.2 g of gallic acid, 6 g of anhydrous ethanol and 2 g of glycerol and stir until clear to obtain a mixed solution;

[0071] (4) After the reaction, the agar aqueous solution and the mixed solution are mixed and stirred for 1 hour to obtain a complete reaction solution;

[0072] (5) A silicone oil mixture (prepared by mixing 4 g of diamino silicone oil and 1 g of amino silicone oil) was added to the completely reacted solution, inverted in a 10 cm*10 cm*1 mm polytetrafluoroethylene mold, and frozen at -20°C for 24 h to obtain a hydrogel dressing.

[0073] The hydrogel dressing is thawed in an oven at 30°C. During this process, the hydrogel dressing can achieve orientation distribution and a sticky-slip synergistic effect at the interface in contact with the polytetrafluoroethylene mold, thereby achieving the effect of painlessly removing blood scabs while fitting and covering the wound.

[0074] Experimental Example 1

[0075] 1. Determination of transient adhesion of hydrogel dressings to different substrates

[0076] like Figure 1 As shown, the instantaneous adhesion test results of the hydrogel dressing prepared in Example 1 are shown. The hydrogel dressing prepared in Example 1 is attached to iron ( Figure 1 Aluminum ( Figure 1 Medium B), PP ( Figure 1 Middle C), glass ( Figure 1 D) and PET ( Figure 1 E) can be instantly adhered, and after adhesion, iron, aluminum, PP, glass and PET can be lifted, with good adhesion; Figure 1 As shown in F, the hydrogel dressing prepared in Example 1 adheres tightly to the skin and has the same color as the skin, showing the performance of skin-like hydrogel.

[0077] like Fig. 9 As shown, the instantaneous adhesion test results of the hydrogel dressing prepared in Example 2 are shown. The hydrogel dressing prepared in Example 2 is attached to iron ( Fig. 9 A), aluminum ( Fig. 9 Medium B), PP ( Fig. 9 Middle C), glass ( Fig. 9 D) and PET ( Fig. 9 E) can be instantly adhered, and after adhesion, iron, aluminum, PP, glass and PET can be lifted, with good adhesion; Fig. 9 As shown in F, the hydrogel dressing prepared in Example 2 adheres tightly to the skin and exhibits the properties of a hydrogel.

[0078] like Fig.16 As shown, the instantaneous adhesion test results of the hydrogel dressing prepared in Example 3 are shown. The hydrogel dressing prepared in Example 3 is attached to iron ( Fig.16 Aluminum ( Fig.16 Medium B), PP ( Fig.16 Middle C), glass ( Fig.16 D) and PET ( Fig.16 E) can be instantly adhered, and after adhesion, iron, aluminum, PP, glass and PET can be lifted, with good adhesion; Fig.16 As shown in F, the hydrogel dressing prepared in Example 3 adheres tightly to the skin and exhibits skin-like hydrogel properties.

[0079] 2. Determination of the contact angle between the hydrogel dressing surface and water / blood

[0080] like Figure 2 As shown in A, it is the contact angle between the upper surface of the hydrogel dressing prepared in Example 1 and water. Figure 2 As shown in B, it is the contact angle between the upper surface of the hydrogel dressing prepared in Example 1 and the blood. The contact angle test is the hydrophilicity and hydrophobicity of the solid interface. It can be seen that the water contact angle of the gel prepared with gelatin is above 90°, proving that it has a certain hydrophobic effect.

[0081] like Figure 3 As shown in A, it is the contact angle between the lower surface of the hydrogel dressing prepared in Example 1 and water. Figure 3As shown in B, it is the contact angle between the lower surface of the hydrogel dressing prepared in Example 1 and the blood; Figure 3 Middle C and Figure 3 As shown in D, the lower surface of the gel can slide water, proving that the gel can not only adhere to various substrates instantaneously but also has anti-stickiness to liquids, which is a unique gel property of the present invention.

[0082] like Fig.10 Middle A and Fig.10 Shown in B is the contact angle between the upper surface of the hydrogel dressing of Example 2 and water / blood.

[0083] like Fig.11 Middle A, Fig.11 Middle B, Fig.11 Middle C and Fig.11 D in the middle shows the contact angle between the lower surface of the hydrogel dressing of Example 2 and water / blood, as well as the performance of the lower surface of the hydrogel dressing of Example 2 in contact with water.

[0084] like Fig.17 Middle A and Fig.17 Shown in B is the contact angle between the upper surface of the hydrogel dressing of Example 3 and water / blood.

[0085] like Fig.18 Middle A, Fig.18 Middle B, Fig.18 Middle C and Fig.18 D in the middle shows the contact angle between the lower surface of the hydrogel dressing of Example 3 and water / blood, and the performance of the lower surface of the hydrogel dressing of Example 3 in contact with water.

[0086] 3. Determination of the tensile properties of hydrogel dressings

[0087] like Figure 4 As shown, Figure 4 A is the initial state of the hydrogel dressing prepared in Example 1, Figure 4 B is the morphology of the hydrogel dressing prepared in Example 1 after stretching, which proves that the hydrogel dressing prepared in the present invention has good stretching properties.

[0088] like Fig.12 As shown, Fig.12 A is the initial state of the hydrogel dressing prepared in Example 2, Fig.12 B is the morphology of the hydrogel dressing prepared in Example 2 after stretching, which proves that the hydrogel dressing prepared in the present invention has good tensile properties.

[0089] like Fig.19 As shown, Fig.19 A is the initial state of the hydrogel dressing prepared in Example 3, Fig.19 B is the shape of the hydrogel dressing prepared in Example 3 after stretching.

[0090] 4. Determining the softness of hydrogel dressings

[0091] like Figure 5 As shown, Figure 5 A in the figure shows the hydrogel dressing prepared in Example 1 folded in half. Figure 5 B in the figure is the diagonally folded state of the hydrogel dressing prepared in Example 1, which proves that the hydrogel dressing prepared in the present invention has good softness and can be folded at any angle.

[0092] like Fig.13 As shown, Fig.13 A in the figure shows the hydrogel dressing prepared in Example 2 folded in half. Fig.13 B is the diagonally folded state of the hydrogel dressing prepared in Example 2, which proves that the hydrogel dressing prepared in the present invention has good softness and can be folded at any angle.

[0093] like Fig. 20 As shown, Fig. 20 A in the figure shows the hydrogel dressing prepared in Example 3 folded in half. Fig. 20 B is the diagonally folded state of the hydrogel dressing prepared in Example 3, which proves that the hydrogel dressing prepared in the present invention has good softness and can be folded at any angle.

[0094] 5. Determination of the Air Stability of Hydrogel Dressings

[0095] like Figure 6 As shown, this is the morphology of the hydrogel dressing prepared in Example 1 after being placed in the air for 5 days. The hydrogel dressing prepared in the present invention is still soft and does not change color after being placed in the air for 5 days, and has good air stability.

[0096] VI. Determination of blood adhesion properties of hydrogel dressings

[0097] like Figure 7 As shown, blood adheres to the hydrogel dressing prepared in Example 1 ( Figure 7 After the blood coagulated on the surface of the hydrogel dressing, it was observed that the blood did not adhere to the hydrogel dressing ( Figure 7 Middle B).

[0098] like Fig.14 As shown, blood adheres to the hydrogel dressing prepared in Example 2 ( Fig.14 After the blood coagulated on the surface of the hydrogel dressing, it was observed that the blood did not adhere to the hydrogel dressing ( Fig.14 Middle B).

[0099] like Fig.21 As shown, blood adheres to the hydrogel dressing prepared in Example 3 ( Fig.21 After the blood coagulated on the surface of the hydrogel dressing, it was observed that the blood did not adhere to the hydrogel dressing ( Fig.21 Middle B).

[0100] VII. Determination of underwater stability of hydrogel dressings

[0101] like Figure 8 As shown, the hydrogel dressing prepared in Example 1 was immersed in water for 1 hour ( Figure 8 In Figure A, it is found that the hydrogel dressing prepared by the present invention will only absorb water and swell without breaking, and has good underwater stability.

[0102] like Fig.15 As shown, the hydrogel dressing prepared in Example 2 was immersed in water for 1 hour ( Fig.15 In Figure A, it is found that the hydrogel dressing prepared by the present invention will only absorb water and swell without breaking, and has good underwater stability.

[0103] like Fig. 22 As shown, the hydrogel dressing prepared in Example 3 was immersed in water for 1 hour ( Fig. 22 In Figure A, it is found that the hydrogel dressing prepared by the present invention will only absorb water and swell without breaking, and has good underwater stability.

[0104] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for preparing a bio-based anti-adhesion hydrogel dressing, characterized in that: The following steps are involved: (1) mixing the bio-based monomer and water to obtain a bio-based monomer aqueous solution; (2) mixing the bio-based monomer aqueous solution obtained in step (1) with a cross-linking agent, and reacting the mixture to obtain a reacted bio-based monomer aqueous solution; (3) Mixing the natural organic compound, anhydrous ethanol and polyol, and stirring until clear to obtain a mixed solution; (4) mixing the aqueous solution of the bio-based monomer obtained after the reaction in step (2) and the mixed solution obtained in step (3), stirring and reacting to obtain a completely reacted solution; (5) adding the silicone oil mixture to the reaction complete solution obtained in step (4), freezing, and obtaining a hydrogel dressing; The natural organic compound in step (3) is one or more of tannic acid, proanthocyanidin or gallic acid; The bio-based monomer in step (1) is one of gelatin and agar; the temperature of the water in step (1) is 55-60° C.; the mass ratio of the bio-based monomer to water in step (1) is 1-5:10-20; The silicone oil mixture in step (5) is a mixture of two of dimethyl silicone oil, hydroxy silicone oil, vinyl silicone oil, phenyl silicone oil, amino silicone oil or bisamino silicone oil.

2. The preparation method according to claim 1, characterized in that: The mixing ratio of the bio-based monomer aqueous solution and the cross-linking agent in step (2) is calculated according to the mass ratio of the bio-based monomer to the cross-linking agent of 1-5:0.01-1; the cross-linking agent in step (2) is a biological cross-linking agent or a chemical cross-linking agent, the biological cross-linking agent is glutaminase, the enzyme activity of the glutaminase is 100-500 U / g, and the chemical cross-linking agent is genipin and / or glutaraldehyde.

3. The preparation method according to claim 1, characterized in that: The reaction time in step (2) is 1 to 10 hours.

4. The preparation method according to claim 1, characterized in that: The mixing ratio of the natural organic compound, anhydrous ethanol and polyol in step (3) is calculated according to the mass ratio of bio-based monomer, natural organic compound, anhydrous ethanol and polyol of 1~5:0.1~1:5~10:1~5; the polyol in step (3) is one or more of glycerol, propylene glycol or polyethylene glycol.

5. The preparation method according to claim 1, characterized in that: The stirring reaction time in step (4) is 1 to 2 hours.

6. The preparation method according to claim 1, characterized in that: The freezing temperature in step (5) is -20 to -50°C, and the freezing time is 0.5 to 48 hours.

7. The hydrogel dressing prepared by the preparation method according to any one of claims 1 to 6.

8. Use of the hydrogel dressing prepared by the preparation method according to any one of claims 1 to 6 or the hydrogel dressing according to claim 7 in the preparation of biomedical hemostatic materials.

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