Device for promoting in-vitro proliferation and differentiation of stem cells and culture method thereof

Abstract

The invention discloses a device for promoting in-vitro proliferation and differentiation of stem cells and a culture method thereof, and relates to the technical field of stem cell culture. The device for promoting in-vitro proliferation and differentiation of stem cells comprises a device body, the interior of the device body is hollowed out, an opening is formed in the right side of the device body, an L-shaped cultivation device is arranged in the device body, and the right side of the L-shaped cultivation device is higher than the left side of the L-shaped cultivation device. According to the device for promoting in-vitro proliferation and differentiation of stem cells and the culture method of the device, a pushing plate can be controlled to move through a control frame by arranging a pushing block, at the moment, a closed cover plate is in a vertical state, light emitted by a light-emitting lamp panel cannot be blocked by the closed cover plate, and therefore the irradiation effect cannot be affected; the control frame pushes the sealing cover plate to rotate through the pushing plate, and at the moment, the sealing cover plate seals the top of the cultivation notch, so that impurities in external air can be prevented from flying into the cultivation notch.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell cultivation, in particular to a device for promoting the proliferation and differentiation of stem cells in vitro and a cultivation method thereof. Background Art

[0002] Stem cells are a type of cell with unlimited or immortal self-renewal capabilities and can produce at least one type of highly differentiated daughter cells. Recent studies on stem cell cultivation have found that near-infrared laser irradiation on adult stem cells derived from human fat can increase the replication rate of stem cells by 54%, while green lasers can convert stem cells into different types of cells at a faster rate. Under laboratory conditions, continuous laser irradiation of adult stem cells will lead to increased proliferation and differentiation, thereby improving the efficiency of proliferation and differentiation.

[0003] Existing stem cell irradiation requires storing the culture fluid containing stem cells inside a culture dish, and then directly irradiating with near-infrared laser and green light laser. When this method is used, the culture dish cover used for sealing will produce a certain reflection and refraction of the light, thereby affecting the proliferation and differentiation of stem cells in the culture dish, and also causing large errors in experimental data. When using uncovered culture dishes for cultivation, impurities in the outside air can easily float into the culture dish during the process of taking out the culture dish, which will also cause experimental errors and have certain defects. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a device for promoting the proliferation and differentiation of stem cells in vitro and a culture method thereof, which solves the problems raised in the above-mentioned background technology.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: a device for promoting the proliferation and differentiation of stem cells in vitro, comprising a device body, the interior of the device body is hollowed out and an opening is provided on the right side, an L-shaped cultivation device is arranged inside the device body, the height of the right side of the L-shaped cultivation device is greater than the height of the left side, and a plurality of cultivation recesses are provided on the top of the horizontal part of the L-shaped cultivation device, a light-emitting lamp board is fixed to the top of the inner wall of the device body, a closed cover plate is hingedly installed on the top of the right side of the cultivation recess through a rotating shaft, the right side of the closed cover plate contacts with a push plate, the push plate is fixedly installed on the top of the control frame, the control frame is slidably installed on the top of the horizontal part of the L-shaped cultivation device, an elastic member is provided between the right side of the control frame and the L-shaped cultivation device, and the left side conflicts with a push block, and the push block is fixed inside the device body.

[0006] Preferably, the closing cover is arranged vertically and a torsion spring is provided at the hinge between the closing cover and the cultivation recess, and the top of the rotating shaft and the bottom of the pushing plate are located in the same horizontal plane.

[0007] Preferably, an elastic member is provided between the right side of the control frame and the L-shaped cultivation device, and the elastic force of the elastic member is greater than the elastic force of the torsion spring between the closing cover plate and the cultivation recess.

[0008] Preferably, an exhaust channel is opened at the bottom of the L-shaped cultivation device, and a deceleration device is arranged inside the exhaust channel, and the deceleration device includes a rotating push plate, an elastic deceleration sheet, a matching plate and an elastic bag. The rotating push plate is rotatably installed on the top of the inner wall of the exhaust channel, the elastic deceleration sheet is fixed to the bottom of the rotating push plate and the bottom end of the elastic deceleration sheet is in contact with the bottom of the inner wall of the device body, the matching plate is fixed to the bottom of the elastic bag, and the matching plate is in contact with the rotating push plate, the elastic bag is fixed to the top of the inner wall of the exhaust channel, and a non-Newtonian fluid is arranged inside the elastic bag.

[0009] Preferably, a resistance reducing device is provided at the bottom right side of the device body, and the resistance reducing device includes a rotating shaft and a scraper blade. The rotating shaft is rotatably connected to the device body, the top of the scraper blade is in contact with the bottom of the rotating shaft, a channel is opened between the two sides of the scraper blade, and an air flow channel is opened on the part of the device body located on the left side of the rotating shaft, a protruding film is fixed on the top of the air flow channel, and the end of the air flow channel away from the protruding film points to the scraper blade.

[0010] Preferably, a pulling block is fixed on the left side of the top of the elastic retarder, and the end point of the pulling block is connected to the slider through an elastic connecting strip. The slider is slidably installed on the left side of the rotating push plate, and a knocking block is fixed on one side of the slider, and the knocking block is in contact with the pulling block.

[0011] Preferably, a friction device is provided inside the device body, and the friction device includes a friction strip and a mounting strip, the two ends of the friction strip are respectively fixed to the bottom ends of the two mounting strips, the top of the mounting strip is fixed to the top of the inner wall of the device body, and a recess is provided on the top of the push plate and a friction column is fixed inside the recess.

[0012] Preferably, a scraping and removing strip is provided on the left side of the friction cylinder, and the scraping and removing strip is fixed inside the recess at the top of the push plate.

[0013] Preferably, a hollow friction capsule is fixed at the bottom of the friction strip, and the height of the bottom of the hollow friction capsule is smaller than the height of the top of the push plate.

[0014] A culture method for promoting proliferation and differentiation of stem cells in vitro, comprising: Step 1: Pull out the L-shaped cultivation device from the device body, and the push block is separated from the control frame. The control frame moves leftward under the action of the spring and the push plate pushes the closed cover plate to rotate to a horizontal state, and the top of the cultivation recess is closed by the closed cover plate; Step 2: After the L-shaped culture device is removed from the device body, the control frame is manually pushed to reopen the closed cover, and the stem cells and nutrients are injected into the culture recess; Step 3: Push the L-shaped cultivation device into the main body of the device, the push block will push the control frame to move, the control frame will drive the push plate to separate from the closed cover plate, and the closed cover plate will rotate to open; Step 4: Turn on the light panel, and the near-infrared and green lasers emitted by the light panel will directly irradiate the stem cells in the culture recess.

[0015] The present invention provides a device for promoting the proliferation and differentiation of stem cells in vitro and a culture method thereof. It has the following beneficial effects: (1) The device for promoting the proliferation and differentiation of stem cells in vitro and the culture method thereof, by setting a pushing block, the pushing plate can be controlled to move by the control frame, at this time, the closed cover plate is in a vertical state, the light emitted by the light-emitting lamp board will not be blocked by the closed cover plate, thereby not affecting the irradiation effect, when the L-shaped culture device is moved out of the device body, the control frame pushes the closed cover plate to rotate through the pushing plate, at this time, the closed cover plate closes the top of the culture recess, thereby preventing impurities in the outside air from floating into the culture recess.

[0016] (2) The device for promoting the proliferation and differentiation of stem cells in vitro and the culture method thereof, by providing a rotating push plate, can be rotated when the L-shaped culture device enters the interior of the device body. At this time, the elastic retarder will contact with the bottom of the device body and rely on its own elastic force to achieve a deceleration effect. When the L-shaped culture device moves too fast, the rotating push plate will generate a faster force on the elastic bag through the matching plate. At this time, the non-Newtonian fluid inside the elastic bag solidifies instantly and blocks the continued rotation of the rotating push plate, thereby slowing down the speed at which the L-shaped culture device enters the interior of the device body and preventing the culture solution inside the culture recess from shaking and spilling.

[0017] (3) The device for promoting the proliferation and differentiation of stem cells in vitro and the culture method thereof can prevent excessive friction between the left side of the elastic retarder and the right side of the device body by providing a resistance reduction device. While relying on the elasticity of the elastic retarder to decelerate the L-shaped culture device, the wear of the left side of the elastic retarder and the right side of the bottom of the device body can also be reduced, thereby increasing the service life of the retarder. The protruding film and the air flow channel can be used to blow air to the scraper by the movement of the rotating push plate, so that impurities scraped off the rotating shaft by the scraper are blown out.

[0018] (4) In the device for promoting the proliferation and differentiation of stem cells in vitro and the culture method thereof, the static electricity generated by the friction between the friction strip and the friction column can adsorb impurities on the top of the closed cover plate, thereby preventing the impurities on the top of the closed cover plate from entering the culture recess on the right side thereof and causing contamination when the closed cover plate is flipped over. At the same time, the friction strip can also directly push the impurities on the top of the closed cover plate, thereby pushing the impurities into the recess on the top of the push plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a half-section view of the whole of the present invention; Figure 3 For the present invention Figure 2 The enlarged structural diagram of part A in the middle; Figure 4 It is a left view of the L-shaped cultivation device of the present invention; Figure 5 A half-section diagram of the main body of the device in the present invention; Figure 6 For the present invention Figure 5 The enlarged structural diagram of part B in the middle; Figure 7 It is a bottom view of the retarding device in the present invention; Figure 8 It is a half-section view of the auxiliary stabilizing device in the present invention.

[0020] In the figure: 1. device body; 11. light-emitting lamp board; 12. push block; 13. air flow channel; 14. protruding film; 2. L-shaped cultivation device; 21. cultivation recess; 22. closed cover plate; 23. control frame; 24. push plate; 241. friction cylinder; 242. scraping removal strip; 3. exhaust channel; 4. retarder; 41. rotating push plate; 411. slider; 412. knock block; 42. elastic retarder; 421. pull block; 43. matching plate; 44. elastic capsule; 5. resistance reduction device; 51. rotating shaft; 52. scraper; 6. friction device; 61. friction strip; 611. hollow friction capsule; 62. installation strip. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] See also Figure 1-8The present invention provides a technical solution: a device for promoting the proliferation and differentiation of stem cells in vitro, comprising a device body 1, the interior of the device body 1 is hollowed out and an opening is provided on the right side, an L-shaped cultivation device 2 is arranged inside the device body 1, the height of the right side of the L-shaped cultivation device 2 is greater than the height of the left side, and a plurality of cultivation recesses 21 are provided on the top of the horizontal part of the L-shaped cultivation device 2, a light-emitting lamp board 11 is fixed on the top of the inner wall of the device body 1, a closed cover plate 22 is hingedly installed on the top of the right side of the cultivation recess 21 through a rotating shaft, the right side of the closed cover plate 22 contacts with a push plate 24, the push plate 24 is fixedly installed on the top of a control frame 23, the control frame 23 is slidably installed on the top of the horizontal part of the L-shaped cultivation device 2, an elastic member is provided between the right side of the control frame 23 and the L-shaped cultivation device 2, and the left side contacts with a push block 12, and the push block 12 is fixed inside the device body 1. By setting the pushing block 12, the pushing plate 24 can be controlled to move through the control frame 23. At this time, the closed cover 22 is in a vertical state, and the light emitted by the light panel 11 will not be blocked by the closed cover 22, thereby not affecting the irradiation effect. When the L-shaped cultivation device 2 is moved out of the device body 1, the control frame 23 pushes the closed cover 22 to rotate through the pushing plate 24. At this time, the closed cover 22 closes the top of the cultivation recess 21, thereby preventing impurities in the outside air from floating into the cultivation recess 21.

[0023] Preferably, in this embodiment, the closing cover 22 is vertically arranged and a torsion spring is provided at the hinge between the closing cover 22 and the cultivation recess 21, the elastic force of the torsion spring is smaller than the elastic force of the elastic member, and the top of the rotating shaft and the bottom of the pushing plate 24 are located in the same horizontal plane.

[0024] Preferably, in this embodiment, an elastic member is provided between the right side of the control frame 23 and the L-shaped cultivation device 2 , and the elastic force of the elastic member is greater than the elastic force of the torsion spring between the closing cover 22 and the cultivation recess 21 .

[0025] Preferably, in the present embodiment, an exhaust channel 3 is opened at the bottom of the L-shaped cultivation device 2, and a deceleration device 4 is arranged inside the exhaust channel 3. The deceleration device 4 includes a rotating push plate 41, an elastic deceleration sheet 42, a matching plate 43 and an elastic capsule 44. The rotating push plate 41 is rotatably installed on the top of the inner wall of the exhaust channel 3, the elastic deceleration sheet 42 is fixed at the bottom of the rotating push plate 41 and the bottom end of the elastic deceleration sheet 42 is in contact with the bottom of the inner wall of the device body 1, the matching plate 43 is fixed at the bottom of the elastic capsule 44, and the matching plate 43 is in contact with the rotating push plate 41, the elastic capsule 44 is fixed at the top of the inner wall of the exhaust channel 3, and a non-Newtonian fluid is arranged inside the elastic capsule 44. By setting a rotating push plate 41, the L-shaped cultivation device 2 can be rotated during the process of entering the interior of the device body 1. The elastic deceleration plate 42 will contact the bottom of the device body 1 and rely on its own elastic force to achieve a deceleration effect. When the movement speed of the L-shaped cultivation device 2 is too fast, the rotating push plate 41 will generate a faster force on the elastic bag 44 through the matching plate 43. At this time, the non-Newtonian fluid inside the elastic bag 44 solidifies instantly and blocks the continued rotation of the rotating push plate 41, thereby slowing down the speed of the L-shaped cultivation device 2 entering the interior of the device body 1 and preventing the culture solution inside the cultivation recess 21 from shaking and spilling.

[0026] Preferably, in this embodiment, a resistance reduction device 5 is provided at the bottom of the right side of the device body 1, and the resistance reduction device 5 includes a rotating shaft 51 and a scraper 52. The rotating shaft 51 is rotatably connected to the device body 1, and the top of the scraper 52 contacts the bottom of the rotating shaft 51. A channel is provided between the two sides of the scraper 52. The portion of the device body 1 located on the left side of the rotating shaft 51 is provided with an air flow channel 13, and a protruding film 14 is fixed on the top of the air flow channel 13, and the end of the air flow channel 13 away from the protruding film 14 points to the scraper 52. By providing the resistance reduction device 5, excessive friction between the left side of the elastic retarder 42 and the right side of the device body 1 can be prevented. While the elastic retarder 42 itself is used to decelerate the L-shaped cultivation device 2, the wear degree of the left side of the elastic retarder 42 and the right side of the bottom of the device body 1 can also be reduced, thereby improving the service life of the retarder 4. The protruding film 14 and the air flow channel 13 can be used to blow air to the scraper 52 by the movement of the rotating push plate 41, so that the impurities scraped off the rotating shaft 51 by the scraper 52 are blown out.

[0027] Preferably, in this embodiment, a pull block 421 is fixed to the left side of the top of the elastic retarder 42, and the end point of the pull block 421 is connected to the slider 411 through an elastic connecting strip, and the slider 411 is slidably installed on the left side of the rotating push plate 41, and a knocking block 412 is fixed to one side of the slider 411, and the knocking block 412 is in contact with the pull block 421. By setting the pull block 421, the deformation of the elastic retarder 42 can be used to pull the slider 411 to move. At this time, the slider 411 will knock the pull block 421 through the knocking block 412, and when knocking, the bottom end of the elastic retarder 42 is just located at the top center of the rotating shaft 51, that is, the maximum deformation of the elastic retarder 42, and the knocking of the knocking block 412 on the pull block 421 causes the impurities attached to the rotating shaft 51 and the scraper 52 to fall off.

[0028] Preferably, in this embodiment, a friction device 6 is provided inside the device body 1, and the friction device 6 includes a friction strip 61 and a mounting strip 62, the two ends of the friction strip 61 are respectively fixed to the bottom ends of the two mounting strips 62, the top of the mounting strip 62 is fixed to the top of the inner wall of the device body 1, and a notch is provided at the top of the push plate 24, and a friction column 241 is fixed inside the notch, and the friction between the friction column 241 and the friction strip 61 can generate static electricity. The static electricity generated by the friction between the friction strip 61 and the friction column 241 can adsorb impurities on the top of the closed cover plate 22, and prevent the impurities on the top of the closed cover plate 22 from entering the cultivation notch 21 on the right side thereof when the closed cover plate 22 is turned over to cause pollution, and at the same time, the friction strip 61 can also directly push the impurities on the top of the closed cover plate 22, so as to push the impurities into the notch at the top of the push plate 24.

[0029] Preferably, in this embodiment, a scraping strip 242 is provided on the left side of the friction cylinder 241, and the scraping strip 242 is fixed inside the notch at the top of the push plate 24. When the friction strip 61 passes through the scraping strip 242, the scraping strip 242 will scrape off the impurities accumulated at the bottom of the friction strip 61 and store them inside the notch, thereby preventing the impurities from falling into the subsequent cultivation notch 21 as the friction strip 61 moves.

[0030] Preferably, in this embodiment, a hollow friction capsule 611 is fixed at the bottom of the friction strip 61, and the bottom height of the hollow friction capsule 611 is less than the top height of the push plate 24. The hollow friction capsule 611 can increase the friction between the friction strip 61 and the friction cylinder 241, thereby improving the static electricity generation effect.

[0031] A culture method for promoting proliferation and differentiation of stem cells in vitro, comprising: Step 1: The L-shaped cultivation device 2 is pulled out from the device body 1, and the push block 12 is separated from the control frame 23. The control frame 23 moves leftward under the action of the spring and the push plate 24 pushes the closed cover plate 22 to rotate to a horizontal state, and the top of the cultivation recess 21 is closed by the closed cover plate 22; Step 2: After the L-shaped culture device 2 is removed from the device body 1, the control frame 23 is manually pushed to reopen the closed cover 22, and the stem cells and nutrients are injected into the culture recess 21; Step 3: Push the L-shaped cultivation device 2 into the device body 1, the push block 12 will push the control frame 23 to move, the control frame 23 drives the push plate 24 to separate from the closed cover plate 22, and the closed cover plate 22 rotates to open; Step 4: Turn on the switch of the light panel 11 , and the near-infrared and green lasers emitted by the light panel 11 will directly irradiate the stem cells in the cultivation recess 21 .

[0032] Working principle: During cultivation, the light-emitting lamp panel 11 is turned on, and the light generated by the light-emitting lamp panel 11 directly irradiates the culture solution inside the cultivation recess 21. After cultivation is completed, the L-shaped cultivation device 2 is directly taken out to the right. During the movement of the L-shaped cultivation device 2, the pushing block 12 will be separated from the control frame 23. The control frame 23 is reset under the action of the elastic member and drives the pushing plate 24 to move to the left. The pushing plate 24 pushes the closed cover plate 22 to rotate to a vertical state. At this time, the closed cover plate 22 will close the top of the cultivation recess 21 to prevent external impurities from entering the cultivation recess 21. During cultivation, the L-shaped cultivation device 2 is pushed into the device body 1, and the pushing block 12 pushes the control frame 23 to move and compress the elastic member. When the pushing plate 24 is separated from the closed cover plate 22, the closed cover plate 22 will rotate to a vertical state under the elastic force of the torsion spring, which will not affect the irradiation effect of light on the culture solution inside the cultivation recess 21. When the L-shaped cultivation device 2 moves into the device body 1, the rotating push plate 41 and the elastic retarding sheet 42 will contact with the right side of the bottom of the device body 1 and the rotating push plate 41 will rotate, and the elastic retarding sheet 42 will be deformed. The elastic retarding sheet 42 applies pressure to the elastic capsule 44 through the matching plate 43. The elastic force of the elastic capsule 44 and the elastic retarding sheet 42 will produce resistance to the movement of the L-shaped cultivation device 2 to slow down the movement speed of the L-shaped cultivation device 2, and prevent the L-shaped cultivation device 2 from moving too fast, causing the culture solution inside the cultivation recess 21 to shake and spill out. When the L-shaped cultivation device 2 moves too fast, the non-Newtonian fluid inside the elastic capsule 44 is subjected to a faster force from the control frame 23, and the non-Newtonian fluid will solidify instantly and block the movement of the control frame 23. At this time, the rotation of the closing cover plate 22 will also be blocked, so the L-shaped cultivation device 2 cannot continue to be pushed into the device body 1, thereby achieving a deceleration effect. When the rotating push plate 41 and the elastic retarder 42 pass the right side of the bottom of the device body 1, the rotating push plate 41 and the elastic retarder 42 will contact the rotating shaft 51 and drive the rotating shaft 51 to rotate. At this time, the rotating shaft 51 will protect the left side of the rotating push plate 41 and the elastic retarder 42 to prevent the left side of the rotating push plate 41 and the elastic retarder 42 from scraping against the right side of the device body 1 and causing a reduction in life. At the same time, the scraper 52 will scrape impurities on the surface of the rotating shaft 51 during the rotation of the rotating shaft 51, and the scraped impurities will remain on the left side of the scraper 52. When the elastic retarder 42 contacts the protruding film 14, it will push the protruding film 14 to deform, and the air inside the protruding film 14 and the air flow channel 13 will be under the action of the deformation pressure. The elastic retarder 42 is discharged downward and acts on the left side of the rotating shaft 51, thereby causing the impurities on the left side of the rotating shaft 51 to move to the right and be discharged; when the elastic retarder 42 is deformed, it will drive the pull block 421 to move, and the moving distance of the pull block 421 away from the elastic retarder 42 is greater than the moving distance of the end close to the elastic retarder 42. At this time, the pull block 421 will pull the slider 411 to move. When the elastic retarder 42 moves to the top center of the rotating shaft 51, the deformation of the elastic retarder 42 is the largest. At this time, the moving distance of the slider 411 is the largest, and the knocking block 412 is driven by the slider 411 to impact the pull block 421. The impact vibration is transmitted to the rotating shaft 51 and the scraper 52 through the pull block 421 and the elastic retarder 42, causing the impurities on the rotating shaft 51 and the scraper 52 to fall off; During the movement of the L-shaped cultivation device 2 to the left, the hollow friction capsule 611 continuously contacts and rubs with the friction cylinders 241 in the plurality of push plates 24. At this time, the hollow friction capsule 611 carries static electricity, which makes the hollow friction capsule 611 adsorb impurities on the top of the closed cover plate 22 when it is located on the top of the closed cover plate 22. At the same time, the bottom of the hollow friction capsule 611 directly contacts the top of the closed cover plate 22 and can directly push the impurities on the top of the closed cover plate 22 to move to the right. The hollow friction capsule 611 passes through the friction cylinders 241. 1, the friction cylinder 241 produces a scraping effect on the bottom of the hollow friction capsule 611, thereby scraping off the impurities at the bottom of the hollow friction capsule 611, and because the bottom height of the hollow friction capsule 611 is less than the top height of the scraping removal strip 242, the scraping removal strip 242 will cause the hollow friction capsule 611 to deform when rubbing against the hollow friction capsule 611. The air pressure inside the hollow friction capsule 611 can increase the friction force between the hollow friction capsule 611 and the scraping removal strip 242 without affecting the movement of the hollow friction capsule 611.

[0033] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for promoting proliferation and differentiation of stem cells in vitro, comprising a device body (1), characterized in that: The interior of the device body (1) is hollowed out and has an opening on the right side. An L-shaped cultivation device (2) is arranged inside the device body (1). The height of the right side of the L-shaped cultivation device (2) is greater than that of the left side. A plurality of cultivation recesses (21) are provided on the top of the horizontal part of the L-shaped cultivation device (2). A light-emitting lamp panel (11) is fixed on the top of the inner wall of the device body (1). A closed cover plate (22) is hingedly mounted on the top of the right side of the cultivation recess (21) via a rotating shaft. The right side of the closed cover plate (22) contacts with a push plate (24). The push plate (24) is fixedly mounted on the top of a control frame (23). The control frame (23) is slidably mounted on the top of the horizontal part of the L-shaped cultivation device (2). The left side contacts with a push block (12). The push block (12) is fixed inside the device body (1).

2. The device for promoting proliferation and differentiation of stem cells in vitro according to claim 1, characterized in that: The closed cover plate (22) is arranged vertically and a torsion spring is arranged at the hinge between the closed cover plate (22) and the cultivation recess (21), and the top of the rotating shaft and the bottom of the pushing plate (24) are located in the same horizontal plane.

3. The device for promoting proliferation and differentiation of stem cells in vitro according to claim 2, characterized in that: An elastic member is provided between the right side of the control frame (23) and the L-shaped cultivation device (2), and the elastic force of the elastic member is greater than the elastic force of the torsion spring between the closing cover plate (22) and the cultivation recess (21).

4. The device for promoting proliferation and differentiation of stem cells in vitro according to claim 1, characterized in that: The L-shaped cultivation device (2) is provided with an exhaust channel (3) at the bottom, and a deceleration device (4) is arranged inside the exhaust channel (3). The deceleration device (4) comprises a rotating push plate (41), an elastic deceleration sheet (42), a matching plate (43) and an elastic capsule (44). The rotating push plate (41) is rotatably mounted on the top of the inner wall of the exhaust channel (3), the elastic deceleration sheet (42) is fixed to the bottom of the rotating push plate (41) and the bottom end of the elastic deceleration sheet (42) contacts the bottom of the inner wall of the device body (1), the matching plate (43) is fixed to the bottom of the elastic capsule (44) and the matching plate (43) contacts the rotating push plate (41), the elastic capsule (44) is fixed to the top of the inner wall of the exhaust channel (3), and a non-Newtonian fluid is arranged inside the elastic capsule (44).

5. The device for promoting proliferation and differentiation of stem cells in vitro according to claim 4, characterized in that: A resistance reducing device (5) is provided at the bottom right side of the device body (1), the resistance reducing device (5) comprising a rotating shaft (51) and a scraper (52), the rotating shaft (51) being rotatably connected to the device body (1), the top of the scraper (52) being in contact with the bottom of the rotating shaft (51), a channel being provided through both sides of the scraper (52), an air flow channel (13) being provided at a portion of the device body (1) located on the left side of the rotating shaft (51), a protruding film (14) being fixed at the top of the air flow channel (13), and an end of the air flow channel (13) away from the protruding film (14) pointing towards the scraper (52).

6. The device for promoting proliferation and differentiation of stem cells in vitro according to claim 4, characterized in that: A pulling block (421) is fixed to the left side of the top of the elastic retarder (42); the end point of the pulling block (421) is connected to the slider (411) via an elastic connecting strip; the slider (411) is slidably mounted on the left side of the rotating push plate (41); a knocking block (412) is fixed to one side of the slider (411); the knocking block (412) is in contact with the pulling block (421).

7. The device for promoting proliferation and differentiation of stem cells in vitro according to claim 1, characterized in that: A friction device (6) is arranged inside the device body (1), and the friction device (6) comprises a friction strip (61) and a mounting strip (62), the two ends of the friction strip (61) are respectively fixed to the bottom ends of two mounting strips (62), the top end of the mounting strip (62) is fixed to the top of the inner wall of the device body (1), and a notch is arranged on the top of the push plate (24), and a friction column (241) is fixed inside the notch.

8. The device for promoting proliferation and differentiation of stem cells in vitro according to claim 7, characterized in that: A scraping and removing strip (242) is provided on the left side of the friction cylinder (241), and the scraping and removing strip (242) is fixed inside a notch at the top of the pushing plate (24).

9. The device for promoting proliferation and differentiation of stem cells in vitro according to claim 7, characterized in that: A hollow friction capsule (611) is fixed to the bottom of the friction strip (61), and the bottom height of the hollow friction capsule (611) is less than the top height of the push plate (24).

10. A method for promoting the proliferation and differentiation of stem cells in vitro, characterized in that: include: Step 1: The L-shaped cultivation device (2) is pulled out from the device body (1), the push block (12) is separated from the control frame (23), the control frame (23) moves to the left under the action of the spring and the push plate (24) pushes the closed cover plate (22) to rotate to a horizontal state, and the top of the cultivation recess (21) is closed by the closed cover plate (22); Step 2: After the L-shaped culture device (2) is removed from the device body (1), the control frame (23) is manually pushed to reopen the closed cover (22), and the stem cells and nutrients are injected into the culture recess (21); Step 3: Push the L-shaped cultivation device (2) into the device body (1), the pushing block (12) will push the control frame (23) to move, the control frame (23) drives the pushing plate (24) to separate from the closed cover plate (22), and the closed cover plate (22) rotates to open; Step 4: Turn on the switch of the light panel (11), and the near-infrared and green lasers emitted by the light panel (11) will directly irradiate the stem cells in the cultivation recess (21).

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