Preparation method of siraitia grosvenorii extract dry paste and application of siraitia grosvenorii extract dry paste in radiation damage resistance
Through the preparation and application of Luohan Fruit Extract Dry Paste, the safety and efficiency of existing anti-radiation damage drugs were solved, and the effective recovery of immune function and function in the mouse model of radiation damage was achieved.
Patent Information
- Application Number
- CN202411430398.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-14
- Publication Date
- 2025-02-25
AI Technical Summary
The existing anti-radiation damage drugs are insecure and efficiency, and lack effective traditional Chinese medicine applications to balance human immune function.
The preparation method of Luohan fruit extract dry paste was used to extract and freeze-dry the dry paste of Luohan fruit extract, and the effect of its anti-radiation damage was verified through animal experimental models.
Luohan Fruit Extract Dry Paste significantly improved the mice's weight growth rate, anti-fatigue ability, peripheral blood cell level, organ index and immune-related factor levels, effectively restoring the immune damage and degradation caused by radiation.
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Abstract
Description
Technical Field
[0001] The present invention relates to the field of radiation resistance technology, and in particular to a method for preparing a dried paste of monk fruit extract and an application thereof in radiation resistance. Background Art
[0002] With the rapid development of my country's economy, radiation is widely used in various fields. While benefiting mankind, it also brings inevitable harm and poses a major threat to human health. In recent years, the number of radiation work units has gradually increased, and more and more working groups are vulnerable to potential mild radiation damage, such as aerospace workers, scientific researchers, medical workers, etc.
[0003] Radiation not only causes significant damage to healthy tissues by destroying DNA and producing reactive oxygen species, but also causes imbalance of human immune function by producing immunostimulatory and immunosuppressive effects, and leads to cancer, physical deformity, and even death. In traditional Chinese medicine theory, radiation belongs to the fire-heat toxic evil, which invades blood vessels, transmits to internal organs, damages qi and consumes yin, or causes dysfunction of related internal organs, imbalance of yin and yang in the body. Therefore, it is very necessary to develop the unique material basis of Chinese medicine to resist radiation damage to balance immune function. The drugs currently developed for resisting radiation damage are very limited, and there are also shortcomings such as strong side effects, high toxicity, and short half-life. Designing safe and efficient radiation protectors is a problem that needs to be solved urgently. Based on this hotspot, this application screens and studies many Chinese medicines, and finds that a kind of Momordica grosvenori extract has good anti-radiation effect.
[0004] Momordica grosvenorii (Swingle) C. Jeffrey is a fruit of a perennial vine of the Cucurbitaceae family, mostly produced in southern my country. It has the effects of relieving sore throat and opening voice, clearing heat and moistening the lungs, and relieving constipation. It is moist and can resist fire, heat and toxicity. Modern studies have shown that Momordica grosvenorii has the effects of anti-oxidation, protecting liver damage, anti-inflammatory, and anti-tumor. At present, there are no research reports on the application of Momordica grosvenorii in anti-radiation damage. The present invention provides a preparation method of a Momordica grosvenorii extract dry paste and its application in anti-radiation damage. Summary of the invention
[0005] In view of the defects of the prior art, the purpose of the present invention is to provide a method for preparing a dry paste of monk fruit extract and its application in resisting radiation damage, so as to solve the problems raised in the above background technology.
[0006] The present invention solves the technical problem by adopting the following technical solution:
[0007] The present invention provides a method for preparing a dried paste of monk fruit extract, comprising the following steps:
[0008] Take dried monk fruit, smash it, put it into a decoction pot, and extract it by decoction method for 1 to 3 times, adding 8-12 times the amount of water each time, decocting for 0.5 to 1.5 hours, filtering through double-layer gauze, combining the filtrate, heating and evaporating to concentrate, and then vacuum drying to obtain monk fruit extract dry paste.
[0009] As a further technical solution of the present invention, the following steps are included:
[0010] Take dried monk fruit, smash it, put it into a decoction pot, and extract it by decoction method for 2 to 3 times, adding 10 times the amount of water each time, decocting for 1 to 1.5 hours, filtering through double-layer gauze, combining the filtrate, heating and evaporating to concentrate, and then vacuum drying to obtain monk fruit extract dry paste.
[0011] Another object of the embodiment of the present invention is to provide an application of a dry paste of monk fruit extract extracted by the preparation method of the dry paste of monk fruit extract as described above in resisting radiation damage, comprising the following steps:
[0012] 1) Animal grouping:
[0013] SPF Kunming (KM) female mice, 4 weeks old, weighing 25±4g, were randomly divided into three groups (n=8): blank control group, model group and treatment group. From the second day of radiation, the treatment group was gavaged with Momordica grosvenori extract every day, with a dose of 1.95mg / g and a single gavage volume of 0.4mL. The control group and model group mice were gavaged with an equal volume of normal saline.
[0014] 2) Irradiation parameters:
[0015] use 60 The mice were irradiated with Coγ-rays at one time, with a distance of 0.933m, a dose rate of 7304mGy / h, an irradiation dose of 4Gy, an irradiation time of 1972s, and a projection area of 10cm×10cm. The mice were subjected to a weight-bearing swimming test on the 7th day after irradiation, and the weight growth rate was measured on the 14th day. The peripheral blood test was performed and the organ index was calculated.
[0016] 4) Experimental content: including weight gain changes, weighted swimming test, blood routine test, organ index, and immune-related factor levels.
[0017] As a further technical solution of the present invention, the experimental contents include:
[0018] 3.1) Changes in weight gain:
[0019] The mice were weighed on the 2nd day after irradiation and again on the 14th day to calculate the weight growth rate, which was calculated as follows: weight growth rate = (weight of mice on the last day - weight of mice on the 1st day) / weight of mice on the 1st day * 100%;
[0020] 3.2) Weighted swimming test:
[0021] On the 7th day after radiation, 0.5 h after gavage, a wire weighing 5.0% of the mouse's body weight was placed 1 cm from the base of the mouse's tail, and the mouse's fur was sprayed with a spray bottle. The mouse was allowed to move freely in a water tank with a depth of 50 cm and a temperature of 28-30°C until exhaustion, and the time was counted. The exhaustion time was the interval of 8 s from the time the mouse was placed in the water to the time it sank into the water and did not float up again.
[0022] 3.3) Routine blood test:
[0023] On the 14th day after irradiation, the mice in each group were weighed, their eyeballs were removed and blood was collected. The blood was placed in a 1.5 mL centrifuge tube containing EDTA, and the number of white blood cells (WBC) and the ratio of neutrophils (NE) and lymphocytes (LY) in the whole blood of the mice in each group were detected using a hematology analyzer.
[0024] 3.4) Organ index:
[0025] On the 14th day after radiation, the mice were killed by dislocating the cervical vertebrae, the thymus was separated, the fat was removed, the blood was washed off with pre-cooled saline, the surface moisture of the organs was absorbed with filter paper, and the weight was calculated to calculate the organ index of the mice, which was organ weight / body weight.
[0026] 3.5) Levels of immune-related factors:
[0027] 3.5.1) Liver IGFBP-3 level:
[0028] On the 14th day after radiation, mice were killed by dislocating the cervical vertebrae, the liver was quickly removed, the fat was removed, and the blood stains were washed off with pre-cooled saline. The surface water of the liver was absorbed with filter paper, and 0.5 g of liver was weighed. According to the liver weight (g): 0.9% saline (mL) = 1:9, the tissue was chopped, mechanically homogenized in an ice water bath, and centrifuged at 2500-3000r / min for 10 minutes. The supernatant was taken to obtain 10% tissue homogenate supernatant, and the IGFBP-3 factor level of the mouse 10% tissue homogenate supernatant was detected according to the instructions of the ELISA kit.
[0029] As a further technical scheme of the present invention, the preparation of the Momordica grosvenori extract for daily oral gavage of the drug administration group: take dried Momordica grosvenori, smash it, put it into a decoction pot, extract it by decoction method for 3 times, add 10 times the amount of water each time, decoct for 1.5 hours, filter through double gauze, combine the filtrate, heat and evaporate to concentrate, and then vacuum dry to obtain a dry paste of Momordica grosvenori extract, take an appropriate amount of the dry paste, add an appropriate amount of water to dissolve it into a Momordica grosvenori extract, so that its mass concentration when administered to mice is 121.875 mg / mL, shake well, and obtain.
[0030] Compared with the prior art, the present invention has the following beneficial effects:
[0031] In view of the limited number of safe and efficient anti-radiation damage drugs, the present application provides a method for preparing a dried paste of monk fruit extract and its application in anti-radiation damage. The present application firstly prepares the monk fruit extract by water decoction and dries it into powder by freeze drying; secondly, establishes 60 The Coγ-ray-induced mild radiation damage mouse model was treated with Monk Fruit extract, which increased the mouse's weight growth rate, anti-fatigue ability, peripheral blood cell levels, organ indexes and immune-related factor levels, achieved the recovery effect on radiation-induced immune damage and functional decline, and had a good effect in resisting radiation damage. BRIEF DESCRIPTION OF THE DRAWINGS
[0032] Figure 1 This is the experimental flow chart of the anti-radiation damage effect of monk fruit extract.
[0033] Figure 2 The graph shows the weight growth rate of mice in each group.
[0034] Figure 3 Figure 3 is a graph of the weight-bearing exhaustive swimming time of mice in each group.
[0035] Figure 4 The figure is a comparison of the number of white blood cells in the blood of mice in each group.
[0036] Figure 5 The figure is a comparison of the number of lymphocytes in the blood of mice in each group.
[0037] Figure 6 This is a comparison chart of the thymus index of mice in each group.
[0038] Figure 7 This is a comparison chart of IGFBP-3 factor levels in liver tissue of mice in each group.
[0039] Figure 8 This is a comparison chart of the weight gain changes of each group of mice in Comparative Example 1.
[0040] Fig. 9 This is a comparison chart of the weight-loaded exhaustive swimming time of each group of mice in Comparative Example 1.
[0041] Fig.10 This is a comparison chart of the thymus index of each group of mice in Comparative Example 1. DETAILED DESCRIPTION
[0042] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0043] Instruments and reagents involved in the embodiments of the present invention
[0044] Thermo Fisher Scientific high performance liquid chromatograph, electronic analytical balance. Mogroside V reference substance, HPLC water is ultrapure water, acetonitrile is chromatographic grade, and other reagents are analytical grade.
[0045] Example 1: Preparation of Momordica grosvenori extract dry paste 1 and determination of its components
[0046] Take 21.89g of dried Momordica grosvenori, smash it, put it into a decoction pot, extract it twice by decoction, add 10 times the amount of water each time, decoct for 1.5h, filter it through double gauze, combine the filtrate, heat and evaporate it, and dry it in vacuum to obtain 6.42g of Momordica grosvenori extract paste, and the paste rate is calculated to be 29.31%. The content of Momordica grosvenori saponin V in the Momordica grosvenori extract paste was 11.64% by HPLC.
[0047] Example 2: Preparation of Momordica grosvenori extract dry paste 2 and determination of its components
[0048] Take 22.43g of dried Momordica grosvenori, smash it, put it into a decoction pot, extract it three times by decoction method, add 10 times the amount of water each time, decoct for 1.5h, filter it through double gauze, combine the filtrate, heat and evaporate it, and vacuum dry it to obtain 7.02g of Momordica grosvenori extract dry paste, and the paste rate is calculated to be 31.30%. The content of Momordica grosvenori saponin V in the Momordica grosvenori extract dry paste was 14.87% by HPLC.
[0049] Example 3: Preparation of Momordica grosvenori extract dry paste 3 and determination of its components
[0050] Take 22.06g of dried Momordica grosvenori, break it, put it into a decoction pot, extract it twice by decoction, add 10 times the amount of water each time, decoct for 1h, filter it through double-layer gauze, combine the filtrate, heat and evaporate it, and vacuum dry it to obtain 5.95g of Momordica grosvenori extract dry paste, and the paste rate is calculated to be 26.97%. The content of Momordica grosvenori saponin V in the Momordica grosvenori extract dry paste detected by HPLC is 9.18%.
[0051] Example 4: Effect of Momordica grosvenori extract on body weight changes in mice
[0052] Preparation of the Momordica grosvenori extract for daily oral administration of the drug administration group: 22.43 g of dried Momordica grosvenori was taken, smashed, put into a decoction pot, extracted 3 times by decoction, 10 times the amount of water was added each time, decocted for 1.5 h, filtered through double gauze, the filtrate was combined, heated and evaporated, and then vacuum dried to obtain a dry paste of Momordica grosvenori extract. The dry paste was taken and dissolved in water to form a Momordica grosvenori extract so that its mass concentration when administered to mice was 121.875 mg / mL, and shaken well to obtain. The Momordica grosvenori extract in this embodiment is prepared using the Momordica grosvenori extract dry paste 2 prepared in Example 2.
[0053] Four-week-old SPF female Kunming (KM) mice weighing 25±4 g were randomly divided into three groups (n=8): control group, model group and drug administration group. 60 The mice in the model group and the treatment group were irradiated with Coγ-rays at a dose of 4Gy. From the second day of radiation, the treatment group was gavaged with monk fruit extract every day at a dose of 1.95mg / g, and the single gavage volume was 0.4mL. The mice in the control group and the model group were gavaged with an equal volume of normal saline. The body weight of mice in each group was measured on the second and fourteenth days after radiation, and the body weight growth rate was calculated. Body weight growth rate = (weight of mice on the 14th day - weight of mice on the first day) / weight of mice on the first day * 100%. The results are shown in the attached Figure 2 As shown, in Figure 2-Figure 10 Compared with the model group: P<0.05:*; P<0.01**; P<0.001:***; P<0.0001:****.
[0054] As the instruction manual Figure 2 The weight growth rate of the model group was lower, and the weight growth rate of the drug-treated group was significantly higher than that of the model group (P<0.0001). The above results show that radiation can slow down the weight growth of mice, and the monk fruit extract prepared in this application can effectively avoid the effect of radiation on the slow weight growth of mice.
[0055] Example 5: Effect on the anti-fatigue ability of mice
[0056] On the 7th day after radiation, 0.5h after gavage, a wire weighing 5.0% of the body weight was placed 1cm from the base of the tail of each group of mice. The mice were sprayed with a spray bottle to wet their fur. They were allowed to move freely in a water tank with a depth of 50cm and a temperature of 28-30℃ until they were exhausted and the time was counted. The time to exhaustion was the interval from the time they were placed in the water to the time they sank into the water and no longer floated up for 8s. The results are shown in the attached Figure 3 shown.
[0057] As the instruction manual Figure 3The weight-exhaustive swimming time of the model group was significantly shorter than that of the control group, and the difference was significant (P<0.05); the weight-exhaustive swimming time of the drug-treated group was significantly higher than that of the model group (P<0.05). The results show that irradiation leads to excessive production of free radicals, which eventually leads to oxidative damage to the cell membrane and fatigue of the body. Monk fruit extract has a significant promoting effect on the physical recovery of mice after irradiation, indicating that the extract has a strong antioxidant capacity, can effectively inhibit the production of free radicals, and has a significant repair effect on cell and mitochondrial damage caused by free radicals, thereby delaying fatigue.
[0058] Example 6: Effects on peripheral blood cell levels in mice
[0059] Radiation can have adverse effects on the hematopoietic system, accompanied by varying degrees of leukopenia. When the body tissue is damaged, inflammation occurs, and peripheral blood lymphocytes are also severely damaged, with a sharp decrease in number. The purpose of this example is to detect the number of white blood cells (WBC) and lymphocytes (LY) in the blood of mice to observe the effect of monk fruit extract on the reduction of blood immune function in mice induced by radiation.
[0060] On the 14th day after irradiation, the mice in each group were weighed and their eyeballs were removed to collect blood, which was placed in a 1.5 mL centrifuge tube containing EDTA. The number of white blood cells (WBC) and lymphocytes (LY) in the whole blood of the mice in each group was measured using a hematology analyzer. The results are shown in the attached figure. Figure 4 , Figure 5 As shown, the levels of WBC and LY in the blood of irradiated mice without medication decreased, while the medication group showed the opposite trend and the differences were significant (P<0.05; P<0.05), indicating that γ rays caused a decrease in blood WBC and LY, which reduced the blood immune function of mice. The monk fruit extract prepared in this application can reverse the imbalance of immune cell levels caused by this radiation, thereby promoting the recovery of blood immune function.
[0061] Example 7: Effect on mouse thymus index
[0062] Irradiation usually causes damage to the organs of the body, causing corresponding changes in their weight. As one of the most important immune organs of the body, the thymus and spleen have the main function of cultivating various T lymphocytes, regulating immune function through the secretion of thymic hormones and cytokines by stromal cells, and differentiating mature T cells. Therefore, the weight of the thymus can reflect the quality of the overall immune function to a certain extent. The purpose of this embodiment is to observe the influence of Momordica grosvenori extract on the decline of the overall immune function of mice caused by radiation by calculating the organ index.
[0063] On the 14th day after radiation, the mice were killed by dislocating the cervical vertebrae, the thymus was separated, the fat was removed, the blood was washed off with pre-cooled saline, the surface moisture of the organs was absorbed with filter paper, and the weight was calculated to calculate the organ index of the mice. Organ index = organ weight / body weight. The results are shown in the attached figure. Figure 6 As shown, the thymus index of the model group was significantly lower than that of the control group and the drug-treated group (P<0.001; P<0.01), which indicates that radiation damages the immune organs, resulting in reduced immunity. For the damage to the immune organs caused by irradiation, the monk fruit extract prepared in this application can significantly reverse the damage to the thymus and improve the immune function of mice.
[0064] Example 8: Effect on IGFBP-3 level in mouse liver tissue
[0065] IGFBP-3 is a growth inhibitor induced by p53 and is radiosensitive. p53 is an important component of the DNA damage repair pathway. Under the stimulation of DNA damage induced by factors such as ionizing radiation, the upregulation of IGFBP-3 dependent on p53 is considered to be part of the DNA damage response. This example aims to observe the effect of monk fruit extract on radiation-induced liver damage by measuring the level of IGFBP-3 factor in mouse liver tissue.
[0066] On the 14th day after radiation, mice were killed by cervical dislocation, the liver was quickly removed, fat was removed, and blood stains were washed with pre-cooled saline. The surface water of the liver was dried with filter paper, and 0.5g of liver was weighed. According to the liver weight (g): 0.9% saline (mL) = 1:9, the tissue was cut into pieces, mechanically homogenized in an ice water bath, centrifuged at 2500-3000r / min for 10min, and the supernatant was taken to obtain 10% tissue homogenate supernatant. The IGFBP-3 factor level of the mouse 10% tissue homogenate supernatant was detected according to the instructions of the ELISA kit. The results are shown in the attached figure. Figure 7 The IGFBP-3 level in the model group was significantly higher than that in the control group and the drug-treated group (P<0.05; P<0.001), indicating that radiation increases the IGFBP-3 level, which may cause DNA damage. The monk fruit extract prepared in the present application can significantly reduce the IGFBP-3 level after radiation, treat DNA damage, and play an anti-radiation role.
[0067] Comparative Example 1: Investigating the dose-effect relationship of the dried extract of Momordica grosvenori prepared by different decoction methods in treating radiation-damaged mice (i.e., the protective effect of the drugs prepared in Examples 1, 2, and 3 on radiation-damaged mice)
[0068] Four-week-old SPF-grade Kunming (KM) female mice, weighing 25±4 g, were randomly divided into 5 groups (n=8): a control group, a model group, and Momordica grosvenori extract dry paste group 1, Momordica grosvenori extract dry paste group 2, and Momordica grosvenori extract dry paste group 3 (wherein, Momordica grosvenori extract dry paste group 1 was obtained in Example 1, Momordica grosvenori extract dry paste group 2 was obtained in Example 2, and Momordica grosvenori extract dry paste group 3 was obtained in Example 3).
[0069] use 60The mice in the model group and the treatment group were irradiated with Coγ-rays at a dose of 4Gy. From the second day of irradiation, each treatment group was gavaged with Momordica grosvenori extract every day, with a dose of 1.95mg / g and a single gavage volume of 0.4mL. The mice in the control group and the model group were gavaged with an equal volume of normal saline. The weight gain, anti-fatigue ability and thymus index of each group of mice were examined. The results are shown in the attached figure. Figure 8-10 shown.
[0070] As the instruction manual Figure 8-10 The weight growth rate, anti-fatigue ability and thymus index of each drug-administered group were significantly better than those of the model group, and this therapeutic effect became more significant as the extraction time and number of extractions of monk fruit increased. The above results show that radiation can slow down the weight growth of mice, reduce their anti-fatigue ability, and damage the immune system of mice by affecting the immune organ thymus. The monk fruit extract prepared in this application can effectively alleviate the harm of irradiation to the mouse body, and this effect can be significantly improved by extending the decoction time and number.
[0071] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.
[0072] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.
Claims
1. A method for preparing a dried paste of monk fruit extract, characterized in that: The following steps are involved: Take dried monk fruit, smash it, put it into a decoction pot, and extract it by decoction method for 1 to 3 times, adding 8-12 times the amount of water each time, decocting for 0.5 to 1.5 hours, filtering through double-layer gauze, combining the filtrate, heating and evaporating to concentrate, and then vacuum drying to obtain monk fruit extract dry paste.
2. The method for preparing a dry paste of Momordica grosvenori extract according to claim 1, characterized in that: The following steps are involved: Take dried monk fruit, smash it, put it into a decoction pot, and extract it by decoction method for 2 to 3 times, adding 10 times the amount of water each time, decocting for 1 to 1.5 hours, filtering through double-layer gauze, combining the filtrate, heating and evaporating to concentrate, and then vacuum drying to obtain monk fruit extract dry paste.
3. An application of the dried extract of Momordica grosvenori extracted by the method for preparing dried extract of Momordica grosvenori extract as claimed in claim 2 in resisting radiation damage, characterized in that: The following steps are involved: 1) Animal grouping: SPF female mice, 4 weeks old, weighing 25±4g, were randomly divided into three groups: blank control group, model group and treatment group. From the second day of radiation, the treatment group was gavaged with Momordica grosvenori extract every day, with a dose of 1.95mg / g and a single gavage volume of 0.4mL. The control group and model group mice were gavaged with an equal volume of normal saline. 2) Irradiation parameters: use 60 The mice were irradiated with Coγ-rays at one time, with a distance of 0.933m, a dose rate of 7304mGy / h, an irradiation dose of 4Gy, an irradiation time of 1972s, and a projection area of 10cm×10cm. The mice were subjected to a weight-bearing swimming test on the 7th day after irradiation, and the weight growth rate was measured on the 14th day. The peripheral blood test was performed and the organ index was calculated. 3) Experimental content: including weight gain changes, weighted swimming test, blood routine test, organ index, and immune-related factor levels.
4. The use of the dried paste of Momordica grosvenori extract in resisting radiation damage according to claim 3, characterized in that: The experimental contents include: 3.1) Changes in weight gain: The mice were weighed on the 2nd day after irradiation and again on the 14th day to calculate the weight growth rate, which was calculated as follows: weight growth rate = (weight of mice on the last day - weight of mice on the 1st day) / weight of mice on the 1st day * 100%; 3.2) Weighted swimming test: On the 7th day after radiation, 0.5 h after gavage, a wire weighing 5.0% of the mouse's body weight was placed 1 cm from the base of the mouse's tail, and the mouse's fur was sprayed with a spray bottle. The mouse was allowed to move freely in a water tank with a depth of 50 cm and a temperature of 28-30°C until exhaustion, and the time was counted. The exhaustion time was the interval of 8 s from the time the mouse was placed in the water to the time it sank into the water and did not float up again. 3.3) Routine blood test: On the 14th day after radiation, the mice in each group were weighed, their eyeballs were removed and blood was collected. The blood was placed in a 1.5 mL centrifuge tube containing EDTA, and the number of white blood cells and the ratio of neutrophils and lymphocytes in the whole blood of the mice in each group were detected using a hematology analyzer. 3.4) Organ index: On the 14th day after radiation, the mice were killed by dislocating the cervical vertebrae, the thymus was separated, the fat was removed, the blood was washed off with pre-cooled saline, the surface moisture of the organs was absorbed with filter paper, and the weight was calculated to calculate the organ index of the mice, which was organ weight / body weight. 3.5) Levels of immune-related factors: 3.5.1) Liver IGFBP-3 level: On the 14th day after radiation, mice were killed by dislocating the cervical vertebrae, the liver was quickly removed, the fat was removed, and the blood stains were washed off with pre-cooled saline. The surface water of the liver was absorbed with filter paper, and 0.5 g of liver was weighed. According to the liver weight (g): 0.9% saline (mL) = 1:9, the tissue was chopped, mechanically homogenized in an ice water bath, and centrifuged at 2500-3000r / min for 10 minutes. The supernatant was taken to obtain 10% tissue homogenate supernatant, and the IGFBP-3 factor level of the mouse 10% tissue homogenate supernatant was detected according to the instructions of the ELISA kit.
5. The use of the dried paste of Momordica grosvenori extract according to claim 3 in resisting radiation damage, characterized in that: Preparation of the Momordica grosvenori extract for daily oral administration of the drug-dosing group: Take dry Momordica grosvenori, smash it, put it into a decoction pot, extract it by decoction method for 3 times, add 10 times the amount of water each time, decoct for 1.5 hours, filter through double-layer gauze, combine the filtrate, heat and evaporate to concentrate, and then vacuum dry to obtain the Momordica grosvenori extract dry paste, take the dry paste, add water to dissolve it into Momordica grosvenori extract, so that its mass concentration when administered to mice is 121.875 mg / mL, shake well, and obtain.