Small molecule peptide, plant growth regulator containing small molecule peptide and application of plant growth regulator

Abstract

The invention relates to a small molecule peptide, a plant growth regulator containing the small molecule peptide and application of the plant growth regulator, and belongs to the technical field of plant peptide hormones. The small molecule peptide is a plant PIP family small molecule peptide, and the amino acid sequence of the small molecule peptide is RFVKHSGP (OH) SPSGPGH. The small molecule peptide can effectively promote the growth of lateral roots of plants, can be used as a plant growth regulator for promoting the growth of the lateral roots of the plants, and is suitable for agricultural application.

Description

Technical Field

[0001] The invention belongs to the technical field of plant peptide hormones and relates to a small molecule peptide, a plant growth regulator containing the small molecule peptide and applications thereof. Background Art

[0002] Lateral roots are the main component of plant roots. As an important organ for plants to absorb water, nutrients and cope with stress, their developmental plasticity plays a vital role in the adaptability of plants to the external environment. The growth and development of lateral roots is affected by the external environment and regulated by endogenous hormones.

[0003] Research in recent years has shown that plant peptide hormones, like classical plant hormones, play an important regulatory role in physiological activities such as plant growth and development. In 1991, tomato systemin was discovered as the first plant peptide hormone, and many plant peptide hormones and their receptors were subsequently identified. Plant sulfonated peptide hormone (PSK) is a pentapeptide hormone containing two tyrosine sulfonated modifications discovered in 1996. Subsequent studies have shown that it has a wide range of regulatory effects on plant growth and development, stress resistance and innate immunity. Plant peptides have the potential to be used as plant growth regulators.

[0004] In the prior art, there are few studies on the effects of plant peptide hormones on the growth and development of plant roots, especially lateral roots. Summary of the invention

[0005] The main purpose of the present invention is to provide a plant PIP family small molecule peptide, which has the effect of promoting the growth of plant lateral roots.

[0006] The present invention adopts the following technical solutions to achieve the above purpose: An artificially synthesized small molecule peptide, wherein the small molecule peptide is a plant PIP family peptide; the amino acid sequence of the small molecule peptide is RFVKHSGP(OH)SPSGPGH, as shown in SEQ No.1.

[0007] The present invention provides the use of the artificially synthesized small molecule peptide in promoting the growth of plant roots.

[0008] In actual use, the artificially synthesized small molecule peptide can be used alone or after adding an auxiliary agent.

[0009] Preferably, the auxiliary agents include but are not limited to plant hormones, minerals, and amino acids.

[0010] Furthermore, the plant hormone may be common plant hormones that promote cell division and plant growth, such as auxin, gibberellin, cytokinin, and brassinolide.

[0011] Furthermore, the minerals include macroelements such as nitrogen, phosphorus and potassium and trace elements such as iron, boron and new metals.

[0012] Furthermore, the amino acids include leucine, threonine, lysine and the like.

[0013] The present invention provides a plant growth regulator, which contains the artificially synthesized small molecule peptide.

[0014] The plant growth regulator can be used to regulate the growth of plant roots.

[0015] Furthermore, the plant growth regulator is mainly used to promote the growth of plant lateral roots.

[0016] The plant growth regulator can also be used to regulate the growth of plant stems and lateral branches.

[0017] The present invention provides a method for using the plant growth regulator, which comprises applying the plant growth regulator containing the artificially synthesized small molecule peptide to the roots of plants.

[0018] Furthermore, the plant growth regulator is applied during the plant seedling stage.

[0019] The present invention has the following beneficial effects: The present invention provides a plant-derived small-molecule peptide, which is obtained from plants, has a simple structure, and is easily absorbed and utilized by plants. After being absorbed by plants, it can effectively promote the growth and development of lateral roots in the plant root system, effectively increase the root system volume, and a developed plant root system is beneficial to its absorption and utilization of nutrients and promotes growth; at the same time, the small-molecule peptide can also inhibit the growth of the plant main stem and branches, and has a certain effect on the synthesis or retention of organic matter in the plant. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 : Comparison of peanut plants and root systems under different treatments in Experiment 1, where C is peanut plants treated with distilled water, and P is peanut plants treated with plant-derived small molecule peptides; Figure 2 : Comparison of the growth of peanut roots treated with different methods in Experiment 1, where C-1 and C-2 were treated with distilled water, and P-1 and P-2 were treated with plant-derived small molecule peptides; Figure 3 : Comparison of peanut lateral root cell sections with different treatments in experiment 1, where C is peanut lateral root cells treated with distilled water, and P is peanut lateral root cells treated with plant-derived small molecule peptides. DETAILED DESCRIPTION

[0021] The present invention is further explained below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, various equivalent modifications to the present invention by those skilled in the art all fall within the scope of protection of the claims of this application.

[0022] Example 1 Artificial synthesis of small molecule peptides The amino acid sequence of the small molecule peptide is RFVKHSGP(OH)SPSGPGH.

[0023] The small molecule peptide was artificially synthesized by a certain company according to the above amino acid sequence.

[0024] Experiment 1: Effects of artificially synthesized small molecule peptides on peanut growth 1. Materials and Methods 1.1 Test materials This experiment was conducted in a potted plant experiment in the laboratory of our school, and the peanut variety tested was Haihua No. 1.

[0025] 1.2 Experimental design Select peanut seeds with full grains, soak them in 20℃ clean water for 12 hours, place the soaked peanut seeds in a shallow dish with water, cover them with a wet towel, and wait for them to germinate in a lightless environment. During the germination process, spray water to keep the towel moist. After the peanut seeds germinate, replace the water in the shallow dish every day until the peanut sprouts grow to about 5 cm, and then transplant them into the substrate for cultivation.

[0026] Peanut buds with basically the same growth were selected and transplanted into plastic pots with substrates, with one plant in each pot. Two treatments were set in this experiment, namely the control group and the test group. After 10 days of planting, the control group was irrigated with clear water, and the test group was irrigated with the artificially synthesized small molecule peptide of the present invention (0.5 μmol / L). Each treatment was repeated 10 times, with one repeat per pot. The rest of the management was the same.

[0027] Four weeks after the treatment, peanut plants were taken to observe the growth of the plants and roots, and root tips were taken to make slices to observe the morphology of epidermal cells in the root tips.

[0028] 1.3 Test results and analysis By comparing peanut plants, it was found that the peanut plants treated with plant-derived small molecule peptides were thicker, the main stem height became shorter, and the side branches became shorter; the root system was larger in volume, and after separation, it was found that the main root was slightly shorter, but the lateral roots grew lushly.

[0029] In addition, by observing the slices of peanut root tip cells with different treatments, it was found that plant-derived small molecule peptides can accelerate root development. After treatment, the epidermal cells of the lateral roots showed a more irregular shape, and the arrangement of the epidermal cells became more compact; the number of vascular columns of the cells increased significantly after being treated with plant-derived small molecule peptides, and the development was more developed. Figure 1-Figure 3 .

[0030] Experiment 2, Field Experiment 1 1. Materials and Methods 1.1 Test materials This experiment was conducted in the experimental field of our school. The soil fertility is uniform and the irrigation and drainage conditions are good. The peanut variety used for the experiment is Haihua No. 1.

[0031] 1.2 Experimental design The experimental field adopted a randomized block arrangement design and set up two treatments, namely the control group and the experimental group.

[0032] The dosage of nitrogen, phosphorus and potassium in each treatment was the same, and peanut-specific compound fertilizer (15:15:15) was used at 500 kg / hm 2 . Fertilizer was applied as a base, and after spreading the fertilizer, the fertilizer was mixed with the soil by rotary tillage. Ridges were planted, with a ridge width of about 30 cm and a ridge width of about 30 cm. One row of peanuts was planted in each ridge, and 50 holes were set in each ridge. Two ridges were set for each treatment, and two seeds were planted in each hole. Thinning was performed 5 days after emergence, leaving one plant in each hole. After thinning, the test group was treated with root irrigation with the plant-derived small molecule peptide of the present invention (0.5 μmol / L, 10 mL / hole), and the control group was treated with root irrigation with distilled water. After 15 days of root irrigation, 6 plants were randomly selected for each treatment, the roots were washed, and the main stem height, side branch length, number of side branches, aboveground fresh weight, underground fresh weight, aboveground dry weight, and underground dry weight were measured.

[0033] 1.3 Test results and analysis By comparing the properties of peanuts, the main stem height and side branch length of the peanuts treated with plant-derived small molecule peptides were shorter than those of the control group; the weight of organic matter in the peanuts was measured after drying, and it was found that the dry weight and wet weight of the peanuts treated with plant-derived small molecule peptides were slightly higher than those of the control group. This result indicates that the plant-derived small molecule peptides of the present invention may have a certain effect on the synthesis or retention of organic matter in peanuts.

[0034] Table 1 Comparison of peanut plant height and other traits under different treatments

Claims

1. An artificially synthesized small molecule peptide, characterized in that: The small molecule peptide is a plant PIP family peptide; the amino acid sequence of the small molecule peptide is RFVKHSGP(OH)SPSGPGH.

2. Use of the artificially synthesized small molecule peptide as claimed in claim 1 in promoting the growth of plant roots.

3. The use according to claim 2, characterized in that The artificially synthesized small molecule peptide is used alone or after adding an auxiliary agent.

4. The use according to claim 3, characterized in that The auxiliary agents include but are not limited to plant hormones, minerals, and amino acids.

5. A plant growth regulator, characterized in that: The plant growth regulator contains the artificially synthesized small molecule peptide according to claim 1.

6. The plant growth regulator according to claim 5, characterized in that The plant growth regulator is used for regulating the growth of plant roots.

7. The plant growth regulator according to claim 6, characterized in that The plant growth regulator is used for promoting the growth of plant lateral roots.

8. The plant growth regulator according to claim 5, characterized in that The plant growth regulator is used for regulating the growth of plant stems and side branches.

9. A method for using the plant growth regulator according to any one of claims 5 to 8, characterized in that: The use method is to apply the plant growth regulator containing artificially synthesized small molecule peptides to the roots of plants.

10. The method of use according to claim 9, characterized in that: The plant growth regulator containing artificially synthesized small molecule peptide is applied during the plant seedling stage.

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