A cell culture device for regenerative medicine

Abstract

The application discloses a cell culture device for regenerative medicine, which comprises a culture box, a partition plate, heat-conducting particles, a culture bottle and a temperature controller, the culture box is internally provided with multiple layers of the partition plates, the partition plate is provided with a groove, a plurality of the heat-conducting particles are arranged in the groove, the heat-conducting particles are used for covering the outer periphery of the culture bottle, the partition plate is provided with the temperature controller, the bottom of the partition plate is provided with an air outlet, the air outlet is communicated with the air inlet end of a first pump body through a first pipeline, and the air outlet end of the first pump body is communicated into the culture box. The application is characterized by providing the cell culture device for regenerative medicine, which can accurately control the temperature of cell culture.

Description

Technical Field

[0001] This invention relates to the field of cell culture technology, and in particular to a cell culture device for regenerative medicine. Background Technology

[0002] With the rapid development of stem cell technology, gene editing technology, and tissue engineering technology, cell culture in regenerative medicine has become a key method for disease treatment, tissue repair, and organ regeneration. In the cell culture process of regenerative medicine, in addition to specific culture media, growth factors, and cytokines, precise control of the culture environment is equally crucial. This includes, but is not limited to, strict regulation of parameters such as temperature, humidity, and oxygen concentration to ensure that cells can grow and differentiate normally under optimal conditions.

[0003] However, existing temperature control methods still have many shortcomings in controlling cell culture temperature. Direct heating typically heats the bottom of the cell culture flask and then conducts heat through the inside of the flask. This method often leads to uneven temperature distribution inside the flask, affecting cell growth. While air heating can directly conduct heat to the sides of the flask, airflow is easily blocked, especially when multiple flasks are placed in the incubator, making temperature differences between different areas more pronounced and difficult to control precisely. Therefore, existing temperature control methods have certain limitations in regenerative medicine cell culture and cannot meet the demand for high-precision temperature control. Summary of the Invention

[0004] In view of the above-mentioned prior art, the present invention provides a cell culture device for regenerative medicine that can more precisely control the temperature of cell culture.

[0005] To achieve the above objectives, the technical solution of this invention is implemented as follows:

[0006] A cell culture device for regenerative medicine includes an incubator, partitions, heat-conducting particles, culture flasks, and a temperature controller. The incubator has multiple partitions, each with a groove containing a plurality of heat-conducting particles that cover the outer periphery of the culture flasks. The partitions are equipped with the temperature controller, and the bottom of each partition has an air outlet. The air outlet is connected to the air inlet of a first pump body via a first pipe, and the air outlet of the first pump body is connected to the incubator.

[0007] Furthermore, the temperature controller includes a heating wire, a controller, and a temperature sensor. The temperature sensor is located within the groove, and the heat-conducting particles cover the temperature sensor. The heating wire is located at the bottom of the partition, and the heating wire and the temperature sensor are signal-connected to the controller.

[0008] Furthermore, the partition is provided with a through hole, and the through hole is provided with an air guide tube. The air guide tube connects the upper and lower sides of the partition, and the top of the air guide tube is flush with the top of the partition.

[0009] Furthermore, a light guide tube is provided in the groove, the light guide tube has a light outlet, the light guide tube is connected to the light cover, and an ultraviolet lamp is provided in the light cover.

[0010] Furthermore, a shielding cover is provided above the partition, and a reflective layer is provided below the shielding cover.

[0011] Furthermore, the incubator is equipped with a second air pump, the air inlet of which is connected to the outside, and the air outlet of which is connected in sequence to a filter and an air sterilization device, the sterilization device being in communication with the inner cavity of the incubator.

[0012] Furthermore, the bottom of the partition is provided with a horizontal plate, the horizontal plate is provided with a plurality of first through holes, the inner diameter of the first through holes is smaller than the diameter of the heat-conducting particles, and the air outlet is located below the horizontal plate.

[0013] Furthermore, the horizontal plate is made of a transparent material, the bottom of the partition is provided with a reflective coating, and an ultraviolet lamp is provided below the horizontal plate.

[0014] Furthermore, one side of the first through hole is perpendicular to the top surface of the horizontal plate, and the other side is smoothly transitioned to the top surface of the horizontal plate. The horizontal plate is slidably connected to the partition plate. The partition plate is provided with a first spring connecting the horizontal plate. The partition plate is provided with a guide block. The guide block is elastic, and the outlet is directly facing the guide block.

[0015] Furthermore, a vertical plate is provided on one side of the partition, the vertical plate is slidably connected to the partition, the partition is provided with a second spring connected to the vertical plate, the vertical plate is provided with a second through hole, the side of the second through hole facing one side is perpendicular to the surface of the vertical plate, the side of the second through hole facing upwards smoothly transitions to the surface of the vertical plate, the vertical plate is rotatably connected to one end of the connecting rod, and the horizontal plate is rotatably connected to the other end of the connecting rod.

[0016] The beneficial effects of this invention are as follows: Heat conduction is achieved by covering the outer periphery of the culture flask with heat-conducting particles, which do not evaporate, thus maintaining the humidity within the incubator and promoting cell culture. A temperature controller heats the heat-conducting particles in the partition, and the heat is then conducted to the culture flask through these particles, achieving effective temperature control and promoting cell growth and differentiation. When the first pump operates, it drives air out of the outlet, promoting heat exchange between the heat-conducting particles and ensuring uniform temperature of the particles within the groove. Simultaneously, air circulates within the incubator, avoiding differences in air quality between different areas, reducing dead zones in airflow, and ensuring the stability of the incubator environment. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of a cell culture device for regenerative medicine in Embodiment 1 of this application;

[0018] Figure 2 This is a schematic diagram of the structure of a cell culture device for regenerative medicine in Embodiment 2 of this application;

[0019] Figure 3 This is a schematic diagram of the connection structure between the horizontal plate and the vertical plate in the embodiment of this application;

[0020] Explanation of icon numbers:

[0021] 1. Incubator; 2. Partition; 3. Heat-conducting particles; 4. Culture flask; 5. Heating wire; 6. Temperature sensor; 7. Air outlet; 8. First pipe; 9. First pump body; 10. Air guide tube; 11. Light guide tube; 12. Light outlet; 13. Light cover; 14. Ultraviolet lamp; 15. Shielding cover; 16. Second air pump; 17. Filter; 18. Air sterilization device; 19. Horizontal plate; 20. First through hole; 21. First spring; 22. Flow guide block; 23. Vertical plate; 24. Second spring; 25. Second through hole; 26. Connecting rod; 27. Groove. Detailed Implementation

[0022] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the invention.

[0023] It should also be noted that when an element is referred to as being "fixed to" another element, it can be directly attached to the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "inner," "outer," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0024] Example 1

[0025] Please refer to the attached document. Figure 1 This application provides a cell culture device for regenerative medicine, including an incubator 1, partitions 2, heat-conducting particles 3, culture flasks 4, and a temperature controller. The incubator 1 contains multiple layers of partitions 2, each partition 2 having a groove 27. A plurality of heat-conducting particles 3 are disposed within the grooves 27, covering the outer periphery of the culture flasks 4. The partitions 2 are equipped with the temperature controller. An air outlet 7 is located at the bottom of each partition 2, connected to the air inlet of a first pump body 9 via a first pipe 8. The air outlet of the first pump body 9 is connected to the incubator 1. During culture, cells are placed into the culture flasks 4, which are then placed into the grooves 27 of the partitions 2. The heat-conducting particles 3 cover the culture flasks 4, providing excellent thermal conductivity and transferring heat into the culture flasks 4. The heat-conducting particles 3 do not evaporate during heat conduction, thus maintaining the humidity within the incubator 1 and effectively controlling the humidity of the culture environment, facilitating cell culture. In cell culture, a temperature controller is used to heat the metal particles in the partition 2, which then conduct heat to the culture flask 4, effectively controlling the temperature of the culture flask 4 and promoting cell growth and differentiation. When the first pump 9 operates, it drives air out of the outlet 7. The air passes through the gaps in the heat-conducting particles 3 and exits from above. The airflow promotes heat exchange between the heat-conducting particles 3, thus ensuring a uniform temperature of the heat-conducting particles 3 within the groove 27. Simultaneously, under the action of the air pump, the air circulates within the incubator 1, promoting a uniform air temperature in contact with the culture flask 4, preventing differences in air quality in different areas, reducing dead zones in airflow within the incubator 1, and ensuring that the environment within the incubator 1 remains within a stable range.

[0026] Specifically, the temperature controller includes a heating wire 5, a controller, and a temperature sensor 6. The temperature sensor is located within the groove 27, and the heat-conducting particles 3 cover the temperature sensor 6. The heating wire 5 is located at the bottom of the partition 2. The heating wire 5 and the temperature sensor 6 are connected to the controller. The controller controls the heating wire 5 to heat the cells, and the temperature sensor 6 monitors the temperature within the groove 27 in real time, thereby precisely controlling the temperature of the culture flask 4, which is beneficial for cell growth and differentiation.

[0027] Specifically, the partition 2 has a through hole, through which an air guide pipe 10 is installed. The air guide pipe 10 connects the upper and lower sides of the partition 2, and the top of the air guide pipe 10 is flush with the top of the partition 2. The air guide pipe 10 connects the upper and lower sides of the partition 2, allowing direct airflow between the upper and lower parts of the partition 2. When the incubator 1 is turned on, raising or lowering the partition 2 allows air to circulate between the upper and lower parts of the partition 2, preventing a large amount of outside air from entering the incubator 1 due to the up-and-down movement of the partition 2, thus reducing the possibility of environmental contamination in the incubator 1. The top of the air guide pipe 10 is parallel to the partition 2, preventing the heat-conducting particles 3 in the groove 27 from falling into the air guide pipe 10.

[0028] Optionally, a light guide tube 11 is provided within the groove 27, and the light guide tube 11 has a light outlet 12. The light guide tube 11 is connected to a light cover 13, and an ultraviolet lamp 14 is provided inside the light cover 13. When the ultraviolet lamp 14 is working, it emits ultraviolet light, which propagates along the light guide tube 11 and then exits from the light outlet 12 to irradiate the heat-conducting particles 3. Under the irradiation of ultraviolet light, bacteria on the heat-conducting particles 3 can be inactivated, reducing contamination in the incubator 1.

[0029] Specifically, a shielding cover 15 is provided above the partition 2, and a reflective layer is provided below the shielding cover 15. After light overflows from the surface of the heat-conducting particles 3, it is reflected back again after passing through the shielding cover 15, thereby improving the sterilization effect of the ultraviolet lamp 14. Preferably, the surface of the heat-conducting particles 3 has a reflective layer, which can reflect ultraviolet light and improve the sterilization effect of ultraviolet light.

[0030] Specifically, the incubator 1 is equipped with a second air pump 16. The air inlet of the second air pump 16 is connected to the outside, and the air outlet of the second air pump 16 is connected in sequence to a filter 17 and an air sterilization device 18. The sterilization device is connected to the inner cavity of the incubator 1. The second air pump 16 draws outside air into the incubator 1. After the door of the incubator 1 is opened, the continuous injection of air into the incubator 1 maintains a slightly higher air pressure inside the incubator 1 than atmospheric pressure, thus preventing outside air from entering the incubator 1 through the opening and preventing contamination. The air drawn by the second air pump 16 is filtered by the filter 17 and then sterilized by the air sterilization device 18 before entering the incubator 1, ensuring the cleanliness of the air entering the incubator 1.

[0031] Example 2

[0032] Please refer to the attached document. Figures 2-3The difference between this embodiment and Embodiment 1 is that the bottom of the partition 2 is provided with a horizontal plate 19, and the horizontal plate 19 is provided with a plurality of first through holes 20. The inner diameter of the first through holes 20 is smaller than the diameter of the heat-conducting particles 3, and the air outlet 7 is located below the horizontal plate 19. The heat-conducting particles 3 cannot pass through the first through holes 20. When the gas entering the area below the horizontal plate 19 from the air outlet 7 flows upward, it needs to pass through the first through holes 20 of the horizontal plate 19. The horizontal plate 19 promotes the uniform flow of air at the bottom, which can improve the uniformity of airflow and reduce the airflow difference between different areas.

[0033] Specifically, the horizontal plate 19 is made of a transparent material, the bottom of the partition 2 is provided with a reflective coating, and an ultraviolet lamp 14 is provided below the horizontal plate 19. The transparent horizontal plate 19 allows light to pass through directly, and the ultraviolet lamp 14 emits ultraviolet light to sterilize the heat-conducting particles 3, thereby eliminating bacteria on the heat-conducting particles 3 and maintaining a stable environment inside the incubator 1.

[0034] Specifically, one side of the first through hole 20 is perpendicular to the top surface of the horizontal plate 19, while the other side smoothly transitions to the top surface of the horizontal plate 19. The horizontal plate 19 is slidably connected to the partition plate 2. The partition plate 2 is provided with a first spring 21 connecting the horizontal plate 19 and a guide block 22. The guide block 22 is elastic, and the outlet faces the guide block 22. When the airflow blows onto the guide block 22, the guide block 22 vibrates under the action of the airflow. This vibration is transmitted to the horizontal plate 19, causing the horizontal plate 19 to vibrate. The reciprocating vibration of the horizontal plate 19 in the horizontal direction generates a thrust on the heat-conducting particles 3. Due to the different frictional forces on the heat-conducting particles 3 on both sides of the first through hole 20, the heat-conducting particles 3 tend to move to the other side during the reciprocating vibration. During continuous vibration, the heat-conducting particles 3 at the bottom will change, allowing different heat-conducting particles 3 to be exposed to ultraviolet radiation. Meanwhile, as the heat-conducting particles 3 move, different sides of the heat-conducting particles 3 will also face the ultraviolet lamp 14, making the heat-conducting particles 3 more evenly exposed to ultraviolet light.

[0035] Specifically, a vertical plate 23 is provided on one side of the partition 2, and the vertical plate 23 is slidably connected to the partition 2. The partition 2 is provided with a second spring 24 connected to the vertical plate 23. The vertical plate 23 is provided with a second through hole 25. The side of the second through hole 25 facing one side is perpendicular to the surface of the vertical plate 23, and the side of the second through hole 25 facing upwards smoothly transitions to the surface of the vertical plate 23. The vertical plate 23 is rotatably connected to one end of the connecting rod 26, and the horizontal plate 19 is rotatably connected to the other end of the connecting rod 26. When the horizontal plate 19 vibrates, it drives the vertical plate 23 to move through the connecting rod 26, causing the vertical plate 23 to vibrate in the vertical direction. The reciprocating vibration of the vertical plate 23 in the vertical direction will generate a thrust on the heat-conducting particles 3. Due to the different frictional forces on both sides of the second through hole 25 against the heat-conducting particles 3, the heat-conducting particles 3 have an upward tendency during the reciprocating vibration. During continuous vibration, the heat-conducting particles 3 located on the side will change, and the heat-conducting particles 3 on the side will move upwards. The horizontal plate 19 pushes the bottom heat-conducting particles 3 towards the side closer to the vertical plate 23, and the vertical plate 23 then pushes the bottom heat-conducting particles 3 upward, thereby forming an effective flow of the heat-conducting particles 3, which allows the heat-conducting particles 3 to come into contact with ultraviolet light for sterilization.

[0036] The above are merely specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. The scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A cell culture device for regenerative medicine, characterized in that, The system includes an incubator, partitions, heat-conducting particles, culture flasks, and a temperature controller. The incubator contains multiple layers of partitions, each with grooves containing a plurality of heat-conducting particles that cover the outer periphery of the culture flasks. The partitions also include the temperature controller. Each partition has an air outlet at its bottom, connected to the air inlet of a first pump via a first pipe. The air outlet of the first pump is connected to the interior of the incubator. When the first pump operates, it drives air out through the air outlet, which then passes through the gaps in the heat-conducting particles and exits from above. A horizontal plate is located at the bottom of the partitions, allowing water to pass through. The flat plate has several first through holes, the inner diameter of which is smaller than the diameter of the heat-conducting particles. The air outlet is located below the horizontal plate. The horizontal plate is made of transparent material, and the bottom of the partition has a reflective coating. An ultraviolet lamp is located below the horizontal plate. One side of the first through hole is perpendicular to the top surface of the horizontal plate, and the other side is smoothly transitioned to the top surface of the horizontal plate. The horizontal plate and the partition are slidably connected. The partition has a first spring connecting the horizontal plate and a flow guide block. The flow guide block is elastic, and the air outlet is directly opposite the flow guide block.

2. The cell culture device for regenerative medicine according to claim 1, characterized in that, The temperature controller includes a heating wire, a controller, and a temperature sensor. The temperature sensor is located in the groove, and the heat-conducting particles cover the temperature sensor. The heating wire is located at the bottom of the partition, and the heating wire and the temperature sensor are signal-connected to the controller.

3. The cell culture device for regenerative medicine according to claim 1, characterized in that, The partition is provided with a through hole, and the through hole is provided with an air guide tube. The air guide tube connects the upper and lower sides of the partition, and the top of the air guide tube is flush with the top of the partition.

4. A cell culture device for regenerative medicine according to claim 1, characterized in that, The groove is provided with a light guide tube, the light guide tube is provided with a light outlet, the light guide tube is connected to the light cover, and the light cover is provided with an ultraviolet lamp.

5. A cell culture device for regenerative medicine according to claim 4, characterized in that, The partition is provided with a shielding cover above it and a reflective layer below it.

6. A cell culture device for regenerative medicine according to claim 1, characterized in that, The incubator is equipped with a second air pump. The air inlet of the second air pump is connected to the outside, and the air outlet of the second air pump is connected in sequence to a filter and an air sterilization device. The sterilization device is connected to the inner cavity of the incubator.

7. A cell culture device for regenerative medicine according to claim 6, characterized in that, A vertical plate is provided on one side of the partition, and the vertical plate is slidably connected to the partition. The partition is provided with a second spring connected to the vertical plate. The vertical plate is provided with a second through hole. The side of the second through hole facing one side is perpendicular to the surface of the vertical plate, and the side of the second through hole facing upward smoothly transitions to the surface of the vertical plate. The vertical plate is rotatably connected to one end of the connecting rod, and the horizontal plate is rotatably connected to the other end of the connecting rod.

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