A rice bud stage real-time monitoring low oxygen stress biomass inhibition flood tolerance identification box

Abstract

The present application relates to the technical field of rice flood tolerance identification, and discloses a rice bud stage real-time monitoring low oxygen stress biomass inhibition flood tolerance identification box, which comprises a test box, a support is fixedly installed in the middle of the test box, a float rod is movably installed at the top of the support, underwater rods are fixedly connected to the lower parts of the two ends of the float rod, float balls are fixedly installed at the end positions of the two ends of the float rod, and lifting grooves are formed in the upper side of the support. The underwater rods arranged at the bottom of the float rod can fix the water flooding depth of the flood tolerance identification test, avoid the absorption or natural evaporation of the culture solution to cause the liquid level to drop and affect the setting of the test water flooding depth, the support and the rotating shaft arranged in the test box can quickly obtain the whole-plant growth ratio of the rice on both sides of the float rod at this period, and the length of the lifting grooves formed in the support gradually decreases from front to back, so that the multiple sets of experimental data can be conveniently observed and recorded.

Description

Technical Field

[0001] This invention relates to the field of rice flood tolerance identification test technology, specifically a flood tolerance identification box for real-time monitoring of biomass inhibition under low oxygen stress during the rice budding stage. Background Technology

[0002] Rice is one of the world's most important food crops, with half of the world's population consuming it. As the world's population gradually increases and the area of ​​high-quality arable land decreases, food security is a perpetual challenge for breeders. Approximately 30% of the world's rice fields are located in low-altitude areas. Even though rice grows in paddy fields and can adapt well to partially submerged environments, waterlogging can still severely affect its growth, development, and yield. Moreover, modern high-yielding rice varieties cannot survive under extreme flooding conditions. Therefore, cultivating flood-resistant rice varieties suitable for growth in low-altitude areas is of great significance for ensuring food security.

[0003] However, in existing rice flood tolerance assessment experiments, the assessment equipment in the laboratories is simple, and the researchers need to replenish the water regularly during the cultivation process to maintain the flood conditions set for different experiments. The experimental errors caused by human factors are relatively large. When measuring the growth of rice seedlings under hypoxia stress, the roots of rice seedlings are very thin and easily broken. Taking the seedlings out to measure the growth has a significant impact on the root system. Especially for rice that has encountered flood hypoxia stress and whose growth condition is already poor, the rice seedlings are often discarded after measuring the growth to avoid the impact of measurement damage on the experimental results. It is not possible to achieve real-time, non-damaging detection of different growth stages of the same plant. Therefore, we need a flood tolerance assessment device that can monitor the inhibition of rice seedling biomass in real time. Summary of the Invention

[0004] This addresses the shortcomings of existing rice flood tolerance testing kits mentioned in the background section during use.

[0005] This invention provides the following technical solution: a flood tolerance identification box for real-time monitoring of biomass inhibition under low oxygen stress in rice during the germination stage, comprising a test box, a drain outlet on the side of the test box, a support fixedly installed in the middle of the test box, a float movably installed at the top of the support, underwater rods fixedly connected to the lower ends of the float, float balls fixedly installed at the ends of the float, a lifting groove opened on the upper side of the support, a rotating shaft movably sleeved in the middle of the lifting groove, a rotating groove opened in the middle of the float, a pointer fixedly connected to the front of the rotating shaft, and scale dials fixedly connected to both sides of the support near the bottom of the support;

[0006] The two ends of the float are symmetrically distributed, and a culture port is opened in the middle of both ends of the float and the underwater rod. A culture net is fixedly installed in the middle of the culture port.

[0007] The underwater rod and the float are movably connected by a screw, and the screw is marked with a scale.

[0008] Preferably, the length of the lifting slots opened on each group of the brackets decreases in a gradient from front to back.

[0009] The present invention has the following beneficial effects:

[0010] 1. This invention achieves a fixed flooding depth in the flood tolerance test by setting an underwater rod at the bottom of the float, avoiding the absorption of the culture medium or natural evaporation that would cause the liquid level to drop and affect the flooding depth. By setting a support and rotating shaft inside the test chamber, the ratio of the total plant growth of rice on both sides of the float at that stage can be quickly obtained. By setting the length of the lifting groove on the support to decrease gradually from front to back, it is easy to observe and record multiple sets of experimental data.

[0011] 2. This invention achieves a fixed flooding depth in the flood resistance test by setting an underwater rod at the bottom of the float, avoiding the absorption of the culture medium or natural evaporation that would cause the liquid level to drop and affect the setting of the flooding depth. The fixed underwater rod is replaced with a movable and adjustable distance between the bottom surfaces of the other floats, thereby achieving free control of the flooding depth in the flood resistance test.

[0012] 3. This invention sets up a support and a rotating shaft inside the test chamber to serve as the central fulcrum of the float. This allows the float to rotate around the rotating shaft under the support of the support after the culture medium is discharged from the test chamber and it is no longer subject to buoyancy. This allows for the rapid determination of the ratio of the total plant growth of rice on both sides of the float at that stage. This process does not damage the root system of the rice plant and does not affect its subsequent cultivation and testing.

[0013] 4. This invention sets the length of the lifting groove on the support to decrease gradually from front to back, and installs it on the front of the test chamber. This allows for observation of the different tilt angles of multiple sets of floats and the ratios indicated by the pointers when conducting rice flood tolerance tests on multiple sets of floats, making it easier to observe and record. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0015] Figure 2 This is a schematic diagram of the cross-sectional structure of the present invention;

[0016] Figure 3 This is a schematic diagram of the measurement state structure of the present invention.

[0017] In the diagram: 1. Test chamber; 12. Drain outlet; 2. Support; 3. Lifting trough; 4. Rotating shaft; 41. Pointer; 5. Float; 51. Culture port; 52. Culture net; 53. Rotating trough; 54. Underwater rod; 6. Dial. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] Please see Figure 1 A flood tolerance identification box for real-time monitoring of biomass inhibition under hypoxia stress in rice during germination includes a test chamber 1. A support 2 is fixedly installed in the middle of the test chamber 1. A float 5 is movably installed at the top of the support 2. Underwater rods 54 are fixedly connected to the lower ends of the float 5. The float 5 is symmetrically distributed on both sides. A culture port 51 is opened in the middle of both ends of the float 5 and the underwater rod 54. A cultivation net 52 is fixedly installed in the middle of the cultivation port 51. The cultivation net 52 can be freely removed from the cultivation port 51. Rice seeds are placed in the cultivation net 52 for experimental cultivation. The ends of the float 5 are... A float is fixedly installed to assist the float 5 in floating on the water surface in the test chamber 1. At this time, the underwater rod 54 is in the submerged position. The cultivation nets 52 on both sides of the float 5 are installed into the cultivation port 51 at the float 5 and the cultivation port 51 at the underwater rod 54, respectively, so that the seeds on both sides of the float 5 can be cultivated at different water submersion depths. This allows for comparison of the growth of the seeds under different water submersion conditions, monitoring the degree of biomass inhibition under hypoxia stress, and fixing the water submersion depth of the water submersion group to avoid the absorption of culture medium or natural evaporation causing the liquid level to drop and affecting the setting of the experimental water submersion depth.

[0020] Please see Figure 2-3The upper side of the support 2 is provided with a lifting groove 3, and a rotating shaft 4 is movably connected in the middle of the lifting groove 3. A rotating groove 53 is provided in the middle of the float 5. The two ends of the rotating shaft 4 are fixedly connected to the middle position of the rotating groove 53, so that the float 5 can move up and down along the lifting groove 3 with the rotating shaft 4. The side of the test chamber 1 is provided with a drain outlet 12. When the drain outlet 12 is opened to drain the culture medium in the test chamber 1, the liquid level in the test chamber 1 drops. The float 5 drops with the liquid level until the rotating shaft 4 moves to the bottom of the lifting groove 3 and stops falling. At this time, the float 5 is far away from the water surface and is no longer supported by buoyancy. It only tilts to the heavier side under the action of gravity. A pointer 41 is fixedly connected to the front of the rotating shaft 4. The two sides of the support 2 are fixedly connected near the bottom of the support 2. The test chamber has a dial 6. When the float 5 tilts due to gravity, it drives the pointer 41 to rotate. The front of the dial 6 is marked with a weight ratio scale, which can quickly determine the ratio of the total plant growth of rice on both sides of the float 5 at that stage. The length of the lifting grooves 3 on each set of supports 2 decreases in a gradient from front to back, which makes it easy to observe the different tilt angles of multiple sets of floats 5 and the ratio indicated by the pointer 41 from the front. After recording, the drain outlet 12 is closed and culture solution is injected into the test chamber 1 again, so that the float 5 rises with the liquid level due to buoyancy. This avoids the problem of plant root damage caused by taking it out to measure the growth during the rice seedling growth stage. It realizes the non-destructive measurement of the ratio of waterlogged and normal plant growth, without affecting its subsequent cultivation and testing.

[0021] In addition, the underwater rod 54 and the float 5 can be connected by a screw, and a scale is set on the screw to adjust the distance between the underwater rod 54 and the float 5. This allows the water depth of the rice planted in the cultivation net 52 on the underwater rod 54 to be freely adjusted, making it suitable for setting different experimental water depths.

[0022] The method of using (working principle) of this invention is as follows:

[0023] In use, adjust the height of the underwater rod 54 from the float 5 to match the test flooding depth. Install the cultivation net 52 into the cultivation ports 51 at both ends of the float 5 and the underwater rod 54, respectively. Place the experimental rice seeds on the cultivation nets 52 at both locations. Inject the culture solution into the test chamber 1. As the culture solution is injected, the float 5 rises under the action of the floats on both sides, causing the rotating shaft 4 to move upward along the lifting groove 3. During this process, the float 5 remains flush with the liquid surface, ensuring that one group of seeds at the float 5 on both sides of the support 2 and the underwater rod 54 is at the liquid level for normal growth, while the other group is at the flooding depth set below the liquid surface, ready for monitoring. When the ratio of the total growth of the two groups of rice seeds is measured, the culture solution in the test chamber 1 is discharged by opening the drain outlet 12. The liquid level in the test chamber 1 drops, and the float 5 drops with the liquid level until the rotating shaft 4 moves to the bottom of the lifting tank 3 and stops falling. At this time, the float 5 is far away from the water surface and is no longer supported by buoyancy. It only tilts to the heavier side under the action of gravity. At this time, the ratio indicated by the pointer 41 on the scale 6 is recorded. During the measurement, it is not necessary to pull the rice roots out of the cultivation net 52, thus avoiding damage to the rice root system during the measurement process. After injecting the culture solution into the test chamber 1 again, the experimental rice can continue to be cultivated, realizing real-time monitoring of the inhibition of plant biomass during the rice budding stage.

[0024] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0025] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A flood tolerance assessment chamber for real-time monitoring of biomass inhibition under hypoxia stress during rice budding stage, comprising a test chamber (1), characterized in that: The test chamber (1) has a drain outlet (12) on its side. A bracket (2) is fixedly installed in the middle of the test chamber (1). A float (5) is movably installed at the top of the bracket (2). Underwater rods (54) are fixedly connected to the bottom of both ends of the float (5). Float balls are fixedly installed at the ends of both ends of the float (5). A lifting groove (3) is opened on the upper side of the bracket (2). A rotating shaft (4) is movably sleeved in the middle of the lifting groove (3). A rotating groove (53) is opened in the middle of the float (5). A pointer (41) is fixedly connected to the front of the rotating shaft (4). A scale (6) is fixedly connected to both sides of the bracket (2) near the bottom of the bracket (2). The two ends of the float (5) are symmetrically distributed. Both the float (5) and the underwater rod (54) have a culture port (51) in the middle of their ends. A culture net (52) is fixedly installed in the middle of the culture port (51). The underwater rod (54) and the float (5) are connected by a screw, and a scale is set on the screw.

2. The flood tolerance identification box for real-time monitoring of biomass inhibition under hypoxia stress during rice budding stage according to claim 1, characterized in that: The length of the lifting groove (3) opened on each set of brackets (2) decreases in a gradient from front to back.

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