Pesticide composition containing pydiflumetofen and metalaxyl-M and application thereof

Abstract

The invention belongs to the technical field of pesticide disinfestation and sterilization, and discloses a pesticide composition containing pydiflumetofen and metalaxyl-M and application thereof, the pesticide composition comprises a bactericide and an insecticide, the bactericide is pydiflumetofen and metalaxyl-M, and the insecticide is fluchlorantraniliprole. According to the pesticide composition disclosed by the invention, the compounds with different action mechanisms are reasonably compounded, and the compounded components have an obvious synergistic effect, so that plant diseases and insect pests can be effectively prevented and treated, the medication frequency and dosage are reduced, the agricultural production cost is reduced, the service life of a single agent is prolonged, and the resistance development of germs and pests is delayed.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide insecticide and sterilization, and discloses a pesticide composition containing pentothiopyrad and metalaxyl-M and application thereof. Background Art

[0002] Pydiflumetofen is a new pyridine amide fungicide, which is a broad-spectrum and highly effective inhibitor of pathogen respiration. Its mechanism of action is to inhibit the function of mitochondria by interfering with the tricarboxylic acid cycle on complex II of the respiratory electron transport chain, preventing them from producing energy, inhibiting the growth of pathogens, and ultimately leading to their death.

[0003] Metalaxy-M, also known as high-efficiency metalaxyl, is an amide fungicide and the world's first commercialized fungicide with stereo-optical activity. It can be used for seed, soil treatment and stem and leaf treatment. Metalaxy-M is a ribosomal RNA synthesis inhibitor and is systemic.

[0004] Fluchlordiniliprole is a new type of diamide insecticide with a paraformylaminobenzamide structure. Fluchlordiniliprole is mainly a stomach poison and has a good preventive effect on a variety of Lepidoptera, Thysanoptera pests and some piercing-sucking pests.

[0005] The most economical and effective method for preventing and treating plant diseases and insect pests at seedling stage at present is to use seed dressing agent to treat seeds. Seed dressing agent is made by processing pesticide original medicine (insecticide, fungicide, etc.), fertilizer, growth regulator, film-forming agent and supporting auxiliary agent through specific process flow, and can be directly or after dilution coated on seed surface to form a pesticide preparation with a protective layer film of certain strength and permeability. It has obvious effects of killing underground pests, preventing and treating seed bacteria and seedling diseases of the invention content, promoting healthy growth and development of seedlings, improving crop quality, increasing seed germination rate, reducing seed usage, and increasing yield. Therefore, the applicant compounded the fungicide fluopicolide, metalaxyl-M and insecticide fluopicolide with different action mechanisms in a reasonable mass ratio and then carried out indoor and field efficacy tests, clarified the control effect of the pesticide composition on seedling diseases and insect pests, and the impact of safety in use, and aimed to provide scientific guidance for the prevention and control of various plant seedling diseases and insect pests and the promotion and use of seed dressing agent. Summary of the invention

[0006] In order to solve the above problems existing in the prior art, the present invention provides a pesticide composition containing fluopicolide and metalaxyl-M and its application. The pesticide composition has obvious synergistic effect on various plant diseases and insect pests, expands the insecticidal and fungicidal spectrum, and can prevent diseases and insect pests at the same time by applying the pesticide once, effectively reducing the amount of pesticide used and the number of applications, thereby alleviating environmental pressure and reducing agricultural production costs.

[0007] In order to achieve the above-mentioned object, the present invention adopts the following technical scheme: a pesticide composition containing fluopicolide and metalaxyl-M, wherein the pesticide composition comprises a fungicide and an insecticide, wherein the fungicide is fluopicolide and metalaxyl-M, and the insecticide is flufenac.

[0008] Furthermore, the mass ratio of fluopicolide to metalaxyl-M in the fungicide is 7:1 to 1:7, or any value within the above numerical range.

[0009] Further, the mass ratio of fluopicolide to metalaxyl-M in the fungicide is 7:1, 6:1, 2:1, 5:3, 3:2, 1:1, 3:4, 1:2, 1:3, 1:5, 1:7, or any value between the above values;

[0010] Furthermore, the mass ratio of fluopicolide to metalaxyl-M in the fungicide is 6:1 to 1:5, or any value within the above numerical range;

[0011] Further, the mass ratio of fluopicolide to metalaxyl-M in the fungicide is 6:1, 2:1, 5:3, 3:2, 1:1, 3:4, 1:2, 1:3, 1:5, or any value between the above values;

[0012] Furthermore, the mass ratios of fluopicolide and metalaxyl-M in the fungicide are 5:3 and 3:2;

[0013] Furthermore, the mass ratio of the fungicide to the insecticide is 4:5 to 1:20, or any value within the above numerical range;

[0014] Furthermore, the mass ratio of the fungicide to the insecticide is 4:5 to 1:8, or any value within the above numerical range;

[0015] Furthermore, the mass ratio of the fungicide to the insecticide is 8:15 to 1:7, or any value within the above numerical range;

[0016] Furthermore, the mass ratio of the bactericide to the pesticide is 8:15, 2:5, 1:3, 8:25, 4:15, 1:4, 8:35, 1:5, 1:6, 1:7, or any value between the above values;

[0017] Further, the mass ratio of fluopicolide to metalaxyl-M to flufenac is 3:2:15, 3:2:20, 3:2:25, 3:2:30, 3:2:35, 5:3:15, 5:3:20, 5:3:25, 5:3:30, 5:3:35, or any value between the above values;

[0018] Furthermore, the mass ratio of fluopicolide to metalaxyl-M to flufenac is 3:2:25, 3:2:30, 5:3:25, 5:3:30, or any value between the above values;

[0019] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the total content of the fungicide in the pesticide composition is 0.5% to 50%, preferably 1% to 10%, and more preferably 2% to 8%.

[0020] Furthermore, based on 100 wt% of the total weight of the pesticide composition, the content of the insecticide in the pesticide composition is 5% to 50%, preferably 10% to 40%, and more preferably 15% to 35%.

[0021] Furthermore, the pesticide composition also includes an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a warning color, a film-forming agent, a synergist and a carrier.

[0022] Further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0023] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0024] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0025] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0026] Disintegrants: The disintegrants are selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0027] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0028] The defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0029] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0030] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0031] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0032] The warning color is selected from any one or more of the adjusted colors of blue, green, red, and purple; and / or

[0033] The film-forming agent is selected from one or more of sodium carboxymethyl starch, cellulose derivatives (sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol), and polyacrylic acid; and / or

[0034] Furthermore, the film-forming agent is selected from seed coating film-forming agent 851, seed coating film-forming agent 805, etc.; and / or

[0035] The synergist is selected from synergist phosphite, synergist ether; and / or

[0036] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0037] Furthermore, the pesticide composition is in the form of any one of a seed treatment suspension, a microcapsule suspension, and a dry seed dressing;

[0038] Furthermore, the pesticide composition is in the form of a seed treatment suspension.

[0039] The present invention also discloses the use of the above pesticide composition in preventing and controlling plant diseases and / or insect pests, wherein the plants include corn, wheat or peanuts;

[0040] The plant diseases are corn stem rot, wheat root rot, wheat stem rot, and peanut root rot;

[0041] The insect pests are white grubs, cutworms, wireworms, fall armyworms and corn borers.

[0042] Corn is one of the main food crops and is of irreplaceable importance in terms of food, feed and industrial use. Corn stalk rot, also known as corn stalk rot and corn bacterial wilt, is a typical soil-borne complex pathogenic disease, with pathogens mainly pathogenic fungi. Corn stalk rot is a disease caused by the single or combined action of multiple pathogenic bacteria. It mainly harms the roots and stem base of corn and can occur throughout the entire growth period of corn. After the disease occurs, the leaves will show three symptoms of green withering, yellowing, and green and yellow withering due to different rates of onset, so it is also called bacterial wilt. In severe cases, it can cause the corn to die completely. According to the different types of pathogens, corn stalk rot can be divided into 8 types, namely Gibberella stalk rot, Fusarium stalk rot, Pythium stalk rot, anthracnose stalk rot, charcoal stalk rot, Botryodiplodia stalk rot, Diplodia stalk rot and bacterial stalk rot. The pathogens of corn stalk rot are complex, and there are many types of pathogens. Different climate and soil factors will lead to significantly different compositions of pathogens, and the infection ability of different dominant pathogens is also significantly different.

[0043] Peanut root rot is a fungal soil-borne disease that harms peanuts and is a worldwide disease. It occurs in various parts of my country, causing serious economic losses and seriously restricting the development of my country's peanut industry. Peanut root rot is mostly caused by infection with Fusarium fungi such as Fusarium solani and Fusarium oxysporum. Root rot can occur throughout the growth period of peanuts, mainly harming the roots and vascular bundles of peanuts, causing the roots to turn brown and rot, followed by the vascular bundles turning brown and rotting, causing poor plant growth, and then causing the whole plant to die. Late infection can harm the pods and cause fruit rot, resulting in the death of most or even all plants, resulting in missing seedlings and broken ridges. The incidence rate is generally 5% to 10%, and in severe cases it can be as high as 30% or more, causing serious economic losses and seriously restricting the development of my country's peanut industry.

[0044] Wheat is one of the main food crops in my country and occupies a major position in food production safety. Therefore, implementing wheat disease prevention and control has become the top priority for my country's food security.

[0045] Wheat root rot is a disease caused by the combined action of multiple fungi. The main pathogens are Cochliobolus graminearum, Fusarium, Helminthosporium, and Helminthosporium. Root rot is widely distributed and occurs in all wheat-growing countries. It mainly occurs in the northern region of China and has been expanding in recent years. It has also been found in the wheat-growing areas of Jiangsu, Zhejiang, Guangdong, and Fujian. The pathogenic bacteria of root rot have a wide host range and can infect wheat and other grass crops and dozens of grass weeds. Root rot not only affects yield, but also reduces the quality and commercial value of wheat.

[0046] Wheat stem rot is an important fungal disease worldwide caused by multiple pathogens such as Fusarium graminearum and Fusarium pseudograminearum. Wheat stem rot is one of the most harmful diseases of wheat, and the most seriously affected part is the wheat root. If wheat seeds are infected by the bacteria before germination, brown spots can be found on the seedling sheath. In severe cases, the seeds will rot or even die directly, eventually leading to a decrease in the emergence rate of wheat fields. The bacteria infect wheat seedlings from the emergence to the greening period. For plants with mild disease, there are no obvious symptoms of disease on the exposed part of the ground, black spots will appear on the coleoptile and the underground stem, and the roots will rot; for plants with more severe disease, the leaves of the seedlings exposed to the outside will turn yellow, the growth and development will be slow, and the plants will be short; in severe cases, the wheat seedlings will turn yellow and eventually die; in mature stage, the disease will occur, and severe plants will produce dead white ears and thin grains.

[0047] Crops are harmed by various diseases and pests from growth to harvest. There are many kinds of underground pests, which are serious, hidden, have complex occurrence patterns, and are difficult to prevent and control. They include white grubs, wireworms, and cutworms, which often cause damage after crop sowing and in the seedling stage, eating crop seeds and rhizomes, causing difficulties in seedling emergence, resulting in plant death, missing seedlings and broken ridges, affecting plant growth, causing production reduction, and causing irreparable losses to agricultural production.

[0048] In order to obtain the desired insecticidal and fungicidal effects, the dosage of the pesticide composition varies depending on various factors, such as the crop to be protected, the type of harmful organisms, the degree of infection, climatic conditions, application site, etc.

[0049] The beneficial effects of the present invention are as follows:

[0050] 1) The pesticide composition of the present invention is compounded with compounds with different action mechanisms in a reasonable ratio, and exhibits obvious synergistic effects on a variety of plant diseases and insect pests;

[0051] 2) The pesticide composition of the present invention can prevent a variety of plant diseases and insect pests, significantly expanding the insecticidal and fungicidal spectrum;

[0052] 3) The pesticide composition of the present invention has a long lasting effect, which effectively reduces the amount of pesticide used and the number of times of pesticide application, thereby alleviating environmental pressure and reducing agricultural production costs. DETAILED DESCRIPTION

[0053] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0054] Preparation method of seed treatment suspension: according to the formula ratio, the active ingredients, auxiliary agents and water are mixed and stirred evenly through high shear, and then sanded by a sander for 2.5 hours to make the average particle size reach 1-5 microns. Finally, the thickener, preservative and film-forming agent are added and continued to be sheared and stirred evenly to obtain the seed treatment suspension.

[0055] Preparation Example:

[0056] Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%)

[0057] Formula composition: 3% fluazifop-butyl, 2% metalaxyl-M, 25% flufenac, 3% tristyrylphenol polyoxyethylene ether phosphate, 3% fatty alcohol polyoxyethylene ether, 3% fatty alcohol ethylene oxide-propylene oxide copolymer, 2% polycarboxylic acid sodium salt, 1% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 5% rose red pigment, 5% propylene glycol, 0.5% silicone defoamer, 0.5% kason, and deionized water to make up the balance.

[0058] Preparation Example 2: 35% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+30%)

[0059] Formula composition: 3% fluazifop-butyl, 2% metalaxyl-M, 30% flufenac, 2% glycerol fatty acid ester polyoxyethylene ether, 1% sodium polycarboxylate, 3% tristyrylphenol polyoxyethylene ether polyoxypropylene ether, 2% fatty alcohol polyoxyethylene ether sodium sulfate, 1% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 5% rose red pigment, 5% propylene glycol, 0.5% silicone defoamer, 0.2% sodium p-hydroxybenzoate, and deionized water to make up the balance.

[0060] Preparation Example 3: 33% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+25%)

[0061] Formula composition: 5% fluazifop-butyl, 3% metalaxyl-M, 25% flufenac, 1% alkyl aromatic polyoxyethylene ether polyoxypropylene ether, 2% fatty alcohol polyoxyethylene ether phosphate, 2% sodium lignin sulfonate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0062] Preparation Example 4: 38% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+30%)

[0063] Formula composition: 5% fluazifop-butyl, 3% metalaxyl-M, 30% flufenac, 2% polyoxyethylene sorbitan monooleate, 5% EO / PO block copolymer, 1% isomeric tridecyl alcohol polyoxyethylene ether, 2% fatty alcohol polyoxyethylene ether phosphate, 1% polyacrylic acid, 0.25% xanthan gum, 6% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0064] Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%)

[0065] Formula composition: 3% fluopicolide, 2% metalaxyl, 4% Guerbet alcohol polyoxyethylene ether, 3% EO / PO block copolymer, 3% castor oil polyoxyethylene ether, 2% naphthalenesulfonate formaldehyde condensate, 1% polyethylene glycol, 0.25% xanthan gum, 4% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 0.2% kason, and deionized water to make up the balance.

[0066] Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%)

[0067] Formula composition: 3% fluopicolide, 25% flufenac, 3% castor oil polyoxyethylene ether, 3% styrene phenol polyoxyethylene ether sulfate, 0.5% sodium lignin sulfonate, 1.5% polyacrylic acid, 0.25% xanthan gum, 5% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0068] Comparative Example 3: 27% metalaxyl-M·flufenac suspension seed coating agent (2%+25%)

[0069] Formula composition: 2% metalaxyl, 25% fluchlorfenapyr, 3% isotridecyl alcohol polyoxyethylene ether, 4% alkylphenol formaldehyde resin polyoxyethylene ether, 1% styrenephenol polyoxyethylene ether phosphate, 2% sodium alginate, 2% sodium lauryl sulfate, 1% sodium carboxymethyl starch, 0.1% xanthan gum, 5% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0070] Comparative Example 4: 33% fluopicolide·flufenac suspension seed coating agent (3%+30%)

[0071] Formula composition: 3% fluopicolide, 30% flufenac, 3% EO / PO block copolymer, 2% fatty alcohol polyoxyethylene ether sulfate, 3% Guerbet alcohol polyoxyethylene ether, 1% polyacrylic acid, 0.2% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance;

[0072] Comparative Example 5: 32% metalaxyl-M·flufenac suspension seed coating agent (2%+30%)

[0073] Formula composition: 2% metalaxyl, 30% flufenac, 3% sorbitan polyoxyethylene ether, 2% alkylphenol formaldehyde resin polyoxyethylene ether, 1% sodium lignin sulfonate, 2% alkylphenol polyoxyethylene ether phosphate, 3% sodium lignin sulfonate, 2% polyvinyl alcohol, 0.2% xanthan gum, 5% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 2% kason, and deionized water to make up the balance.

[0074] Comparative Example 6: 8% fluazifop-butyl·metalaxyl-M suspension seed coating agent (5%+3%)

[0075] Formula composition: 5% fluazifop-butyl, 3% metalaxyl-M, 3% phenethylphenol polyoxyethylene polyoxypropylene ether, 3% alkylphenol polyoxyethylene ether phosphate, 1% naphthalenesulfonate formaldehyde condensate, 1% polyacrylic acid, 0.3% xanthan gum, 5% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0076] Comparative Example 7: 28% metalaxyl-M·flufenac suspension seed coating agent (3%+25%)

[0077] Formula composition: 3% metalaxyl, 25% flufenac, 3% fatty alcohol polyoxyethylene ether, 3% polyoxyethylene sorbitan monooleate, 1% polycarboxylic acid sodium salt, 1% sodium carboxymethyl starch, 0.25% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium sorbate, and deionized water to make up the balance.

[0078] Comparative Example 8: 30% fluopicolide·flufenac suspension seed coating agent (5%+25%)

[0079] Formula composition: 5% fluopicolide, 25% flufenac, 3% phenethylphenol polyoxyethylene polyoxypropylene ether, 2% alkylphenol polyoxyethylene ether phosphate, 1^% arylphenol polyoxyethylene ether phosphate, 2% sodium lignin sulfonate, 1% sodium benzoate, 0.2% xanthan gum, 5% rose red pigment, 5% propylene glycol, 0.25% magnesium aluminum silicate, 0.5% silicone defoamer, 1% polyacrylic acid, and deionized water to make up the balance.

[0080] Comparative Example 9: 35% fluopicolide·flufenac suspension seed coating agent (5%+30%)

[0081] Formula composition: 5% fluopicolide, 30% flufenac, 5% fatty amine polyoxyethylene ether, 3% alkylphenol polyoxyethylene ether phosphate, 3% sodium lignin sulfonate, 2% sodium benzoate, 0.2% xanthan gum, 6% rose red pigment, 5% propylene glycol, 0.5% magnesium aluminum silicate, 1% silicone defoamer, 1% polyacrylic acid, and deionized water to make up the balance.

[0082] Comparative Example 10: 33% metalaxyl-flufenacil suspension seed coating agent (3% + 30%)

[0083] Formula composition: 3% metalaxyl, 30% flufenac, 4% sorbitan polyoxyethylene ether, 5% alkylphenol formaldehyde resin polyoxyethylene ether, 2% fatty alcohol polyoxyethylene ether phosphate, 2% sodium lauryl sulfate, 2% sodium carboxymethyl starch, 0.25% xanthan gum, 5% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0084] Example 1: Indoor activity determination test of pathogens

[0085] The test was conducted in accordance with NY / T 1156.2-2006 “Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Pathogenic Fungal Mycelial Growth”.

[0086] Test targets: Fusarium pesudograminearum, Fusarium oxysporum, Bipolaris sorokiniana, Pythium aphanidermatum.

[0087] Instruments and equipment: electronic balance (sensitivity 0.1 mg), biological incubator, culture dish, pipette, inoculator, hole puncher, caliper, clean bench, alcohol lamp, etc.

[0088] Test agents: Fluoxetine hydroxylamide technical, metalaxyl-M technical, and flufenac technical.

[0089] Preparation of drug mother solution: After dissolving the above raw drugs in appropriate solvents to prepare high-concentration mother solutions, prepare fluopicolide and metalaxyl-M into a mixed solution according to the ratio designed in the experiment, and then dilute the above single agents and mixed agents into 5 series of mass concentrations with 0.1% Tween 80 aqueous solution.

[0090] On this basis, the optimal ratio of fluazifop-butyl and metalaxyl-M was screened out, and the above optimal ratio mixture was used as a fungicide combination, and was ternary compounded with the insecticide fluazifop-butyl according to different mass ratios.

[0091] Test repetition: 4 culture dishes for each concentration of the test agent, 1 culture dish for each repetition, for a total of 4 repetitions, and a 0.1% Tween 80 aqueous solution without the agent was used as a blank control.

[0092] Drug treatment: Under aseptic operation conditions, add the pre-melted sterilized culture medium to the sterile conical flask according to the test treatment, and quantitatively draw the drug solution from low concentration to high concentration, add it to the above conical flask respectively, and shake it thoroughly. Then pour an equal amount into 4 culture media with a diameter of 9 cm to make drug-containing plates of corresponding concentrations. Set the treatment without drug as a blank control, and repeat each treatment 4 times.

[0093] Inoculation: Cut the bacterial cake from the edge of the colony with a sterile puncher under sterile conditions. Use an inoculator to inoculate the bacterial cake in the center of the drug-containing plate with the mycelium side facing up. Cover the plate with a cap and place it in a constant temperature incubator at (25±1)℃ for culture.

[0094] Investigation: Investigate the growth of pathogenic fungi based on the growth of colonies in the blank control culture dish. Use a caliper to measure the diameter of the colonies in centimeters. Use the cross method to measure the diameter of each colony vertically once and take the average value.

[0095] Data statistics and analysis: According to the survey results, the mycelial growth inhibition rate of each treatment concentration on the target bacteria was calculated. The unit is percentage (%) and the calculation result is rounded to two decimal places.

[0096] D=D1-D2

[0097] Where:

[0098] D——colony growth diameter;

[0099] D1——colony diameter;

[0100] D2——diameter of mushroom cake.

[0101]

[0102] Where:

[0103] I——hyphae growth inhibition rate;

[0104] D0——blank control colony growth diameter;

[0105] D T ——The growth diameter of the colony after treatment with chemicals.

[0106] Analyze using statistical analysis system to find regression equation, EC 50 Value, to evaluate the activity of the test agent on the biological test material.

[0107] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0108] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0109]

[0110] Where:

[0111] ATI——Actual Toxicity Index of Mixture;

[0112] S——EC of standard medicine 50 , the unit is milligrams per liter (mg / L);

[0113] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0114] TTI=TI A ×P A +TI B ×P B

[0115] Where:

[0116] TTI – Theoretical Toxicity Index of Mixtures;

[0117] TI A ——A: toxicity index of the agent;

[0118] P A ——The percentage of agent A in the mixture, in percentage (%);

[0119] TI B ——Toxicity index of agent B;

[0120] P B ——The percentage of agent B in the mixture, in percentage (%).

[0121]

[0122] Where:

[0123] CTC – Co-toxicity coefficient;

[0124] ATI——Actual Toxicity Index of Mixture;

[0125] TTI - Theoretical Toxicity Index of Mixture.

[0126] The test results are shown in the following table:

[0127] Table 1 Results of indoor combined toxicity test of different ratios of fluopicolide + metalaxyl-M against Fusarium graminearum

[0128] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin (A) y=4.9007+1.4317x 1.1731 / Metalaxyl(B) / / / A:B(7:1) y=5.0199+1.4114x 0.9681 121.175 A:B(6:1) y=5.0533+1.4103x 0.9166 127.984 A:B(2:1) y=5.1049+1.5753x 0.8578 136.757 A:B(5:3) y=5.1328+1.3791x 0.8011 146.436 A:B(3:2) y=5.2282+1.5175x 0.7073 165.856 A:B(1:1) y=5.2085+1.6771x 0.7511 156.184 A:B(3:4) y=5.1394+1.3967x 0.7947 147.615 A:B(1:2) y=5.1154+1.4136x 0.8286 141.576 A:B(1:3) y=5.0912+1.3564x 0.8566 136.948 A:B(1:5) y=5.0366+1.4508x 0.9436 124.322 A:B(1:7) y=5.0045+1.4481x 0.9929 118.149

[0129] Note: Metalaxyl-M had no significant inhibitory activity against F. graminearum at the designed highest concentration of 200 mg / L, and the EC could not be calculated. 50 and virulence regression equations.

[0130] Table 2 Results of the indoor combined toxicity test of the mixture of fluopicolide + metalaxyl-M and its mixture with fluopicolide against Fusarium graminearum

[0131] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:Metalaxyl-M (A:B=3:2) y=5.2282+1.5175x 0.7073 / Flufenac (C) / / / A:B:C(3:2:10) y=5.3207+1.4656x 0.6042 117.064 A:B:C(3:2:15) y=5.3668+1.5655x 0.5830 121.321 A:B:C(3:2:20) y=5.3904+1.5909x 0.5684 124.437 A:B:C(3:2:25) y=5.4187+1.4595x 0.5165 136.941 A:B:C(3:2:30) y=5.4003+1.4682x 0.5338 132.503 A:B:C(3:2:35) y=5.3833+1.4887x 0.5527 127.972 A:B:C(3:2:40) y=5.3687+1.4838x 0.5643 125.341

[0132] Note: Flufenac has no significant inhibitory activity against F. graminearum at the designed maximum concentration of 200 mg / L, and the EC cannot be calculated. 50 and virulence regression equations.

[0133] The test results in Table 1 and Table 2 show that fluopicolide has a good control effect on Fusarium graminearum. The activity is better when the ratio of fluopicolide: metalaxyl-M is 3:2, and its co-toxicity coefficient is 165.856, with a significant synergistic effect. The mixture of "fluopicolide + metalaxyl-M" (ratio of 3:2) and flufenac in different ratios showed an additive or synergistic effect on Fusarium graminearum, among which the mass ratio of "fluopicolide + metalaxyl-M" mixture to flufenac is (3:2):(15-40), that is, 1:5-1:20, which shows a synergistic effect. The synergistic effect is most significant when the mass ratio of fluopicolide + metalaxyl-M + flufenac is 3:2:25.

[0134] Table 3 Results of indoor combined toxicity test of different ratios of fluopicolide + metalaxyl against Fusarium oxysporum

[0135] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin (A) y=5.8034+1.4375x 0.2761 / Metalaxyl(B) / / / A:B(7:1) y=5.8745+1.5093x 0.2634 104.822 A:B(6:1) y=5.9570+1.4656x 0.2224 124.146 A:B(2:1) y=6.0022+1.4563x 0.2051 134.617 A:B(5:3) y=6.1624+1.5652x 0.1808 152.710 A:B(3:2) y=6.1134+1.5124x 0.1836 150.381 A:B(1:1) y=6.1612+1.6013x 0.1883 146.628 A:B(3:4) y=6.0406+1.4663x 0.1951 141.517 A:B(1:2) y=6.0325+1.4814x 0.2009 137.432 A:B(1:3) y=5.9666+1.4327x 0.2115 130.544 A:B(1:5) y=5.8566+1.2936x 0.2177 126.826 A:B(1:7) y=5.9245+1.4452x 0.2292 120.462

[0136] Note: Metalaxyl-M had no significant inhibitory activity against Fusarium oxysporum at the designed highest concentration of 200 mg / L, and the EC could not be calculated. 50 and virulence regression equations.

[0137] Table 4 Results of the indoor combined toxicity test of the mixture of fluopicolide + metalaxyl-M and its mixture with fluopicolide against Fusarium oxysporum

[0138] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluopicolide:Metalaxyl-M (A:B=5:3) y=6.1624+1.5652x 0.1808 / Flufenac (C) / / / A:B:C(5:3:10) y=6.0689+1.4061x 0.1737 104.088 A:B:C(5:3:15) y=6.1709+1.4002x 0.1458 124.005 A:B:C(5:3:20) y=6.2819+1.5106x 0.1417 127.594 A:B:C(5:3:25) y=6.2649+1.478x 0.1394 129.699 A:B:C(5:3:30) y=6.1999+1.426x 0.1441 125.468 A:B:C(5:3:35) y=6.2287+1.4722x 0.1464 123.497 A:B:C(5:3:40) y=6.1821+1.457x 0.1544 117.098

[0139] Note: Flufenac has no significant inhibitory activity against Fusarium oxysporum at the designed maximum concentration of 200 mg / L, and the EC cannot be calculated. 50 and virulence regression equations.

[0140] The test results in Table 3 and Table 4 show that fluopicolide has a good control effect on Fusarium oxysporum. The activity is better when the ratio of fluopicolide: metalaxyl-M is 5:3, and its co-toxicity coefficient is 152.710, with a significant synergistic effect. The mixed solution of "fluopicolide + metalaxyl-M" (ratio of 5:3) and flufenac in different ratios showed an additive or synergistic effect on Fusarium oxysporum, among which the mass ratio of "fluopicolide + metalaxyl-M" mixture to flufenac is (5:3): (15-35) showing a synergistic effect, and the synergistic effect is most significant when the mass ratio of fluopicolide + metalaxyl-M + flufenac is 5:3:25.

[0141] Table 5 Results of indoor combined toxicity test of different ratios of fluopicolide + metalaxyl-M against Helicoverpa spp.

[0142] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin (A) y=5.7701+1.6296x 0.3368 / Metalaxyl(B) y=4.1626+1.5677x 3.4214 / A:B(7:1) y=5.8446+1.6189x 0.3008 126.189 A:B(6:1) y=5.8827+1.6077x 0.2825 136.846 A:B(2:1) y=5.8079+1.7349x 0.3422 140.707 A:B(5:3) y=5.7545+1.5327x 0.3219 158.070 A:B(3:2) y=5.7906+1.5704x 0.3138 167.866 A:B(1:1) y=5.6011+1.4414x 0.3828 160.197 A:B(3:4) y=5.5776+1.6743x 0.4519 153.726 A:B(1:2) y=5.3773+1.5431x 0.5695 148.235 A:B(1:3) y=5.1714+1.4749x 0.7652 135.919 A:B(1:5) y=4.9467+1.4913x 1.0858 124.723 A:B(1:7) y=4.8023+1.6127x 1.3262 120.283

[0143] Table 6 Results of the indoor combined toxicity test of the mixture of fluopicolide + metalaxyl-M and its mixture with fluopicolide against Helminthosporium spp.

[0144] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:Metalaxyl-M (A:B=3:2) y=6.1586+1.3487x 0.1384 / Flufenac (C) / / / A:B:C(3:2:10) y=6.3331+1.5533x 0.1386 99.856 A:B:C(3:2:15) y=6.3485+1.5264x 0.1308 105.810 A:B:C(3:2:20) y=6.378+1.4483x 0.1118 123.792 A:B:C(3:2:25) y=6.5319+1.5549x 0.1035 133.720 A:B:C(3:2:30) y=6.5825+1.6011x 0.1027 134.761 A:B:C(3:2:35) y=6.5741+1.6438x 0.1103 125.476 A:B:C(3:2:40) y=6.3685+1.4831x 0.1195 115.816

[0145] Note: Flufenac has no significant inhibitory activity against Helicoverpa serrata at the designed maximum concentration of 200 mg / L, and the EC cannot be calculated. 50 and virulence regression equations.

[0146] The test results in Tables 5 and 6 show that both fluopicolide and metalaxyl-M have good control effects on Helicoverpa serrata. The activity of fluopicolide: metalaxyl-M is better when the ratio is 3:2, and its co-toxicity coefficient is 167.866, with significant synergistic effect. The mixture of "fluopicolide + metalaxyl-M" (ratio of 3:2) and different ratios of flufenacet showed additive or synergistic effects on Helicoverpa serrata, among which the mass ratio of "fluopicolide + metalaxyl-M" mixture to flufenacet is (3:2):(20-35), which shows synergistic effect. The synergistic effect is most significant when the mass ratio of fluopicolide + metalaxyl-M + flufenacet is 3:2:30.

[0147] Table 7 Results of indoor combined toxicity test of different ratios of fluopicolide + metalaxyl-M against Pythium aphanidermum

[0148]

[0149]

[0150] Table 8 Results of the indoor combined toxicity test of the mixture of fluopicolide + metalaxyl-M and its mixture with fluopicolide against Pythium aphanidermum

[0151] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:Metalaxyl-M (A:B=3:2) y=4.0677+1.4254x 4.5088 / Flufenac (C) / / / A:B:C(3:2:10) y=3.9826+1.6999x 3.9677 113.638 A:B:C(3:2:15) y=4.1651+1.4719x 3.6918 122.130 A:B:C(3:2:20) y=4.0912+1.6519x 3.5496 127.023 A:B:C(3:2:25) y=4.088+1.7011x 3.4366 131.199 A:B:C(3:2:30) y=4.0873+1.5595x 3.8480 117.173 A:B:C(3:2:35) y=3.9518+1.6385x 4.3624 103.356 A:B:C(3:2:40) y=3.8545+1.6127x 5.1318 87.860

[0152] Note: Flufenac has no significant inhibitory activity against Pythium aphanidermatum at the designed maximum concentration of 200 mg / L, and EC cannot be calculated 50 and virulence regression equations.

[0153] The test results in Tables 7 and 8 show that both fluopicolide and metalaxyl-M have good control effects on Pythium aphanidermatum. The activity of fluopicolide: metalaxyl-M is better when the ratio is 3:2, and its co-toxicity coefficient is 176.980, and the synergistic effect is significant. The mixture of "fluopicolide + metalaxyl-M" (ratio of 3:2) and flufenac in different ratios showed additive or synergistic effects on Pythium aphanidermatum, among which the mass ratio of "fluopicolide + metalaxyl-M" mixture to flufenac is (3:2): (15-25), which shows a synergistic effect. The synergistic effect is most significant when the mass ratio of fluopicolide + metalaxyl-M + flufenacmatum is 3:2:30.

[0154] Example 2: Indoor toxicity test of underground pests

[0155] Test basis: The test refers to NY / T 1154.7-2006 "Guidelines for Indoor Bioassay Tests of Pesticides Insecticides Part 7: Determination of Combined Action of Mixtures".

[0156] Experimental agents: fluazifop-butyl technical, metalaxyl-M technical, flufenac technical.

[0157] Drug preparation: The test drug is prepared into a certain mass of mother solution with a suitable solvent, and the mother solution is diluted in equal proportions into a series of mass concentrations with 0.1% TritonX-80 aqueous solution.

[0158] Test targets: Cutworm (3rd instar larvae), Copper Green Beetle (2nd instar larvae), Wireworm (2nd instar larvae).

[0159] 1. Method for determining the toxicity of small cutworms: Pick the 3rd instar larvae of small cutworms with uniform ages and consistent physiological states and put them into a steel mesh cover. After soaking in the above-mentioned liquid medicine for 10 seconds, take them out and put them on filter paper to dry. Use filter paper to absorb the excess liquid medicine. Repeat 4 times for each treatment, immerse 20 insects in each repetition, and set the treatment without pesticide as a blank control. Transfer the treated test insects to a glass tube containing fresh cabbage as food, cover the tube mouth with a damp black cloth, and place it at 25±1℃ and relative humidity of 65%±5% for breeding and observation. Investigate the death of the test insects 3 days after treatment. The criterion for judging the death of the test insects is that the insect body shrinks significantly or cannot crawl normally after acupuncture.

[0160] 2. Method for determining the toxicity of A. cooperi: Place the 2nd instar larvae of A. cooperi that are healthy, lively and in the same physiological state into an insect immersion device, immerse them in the drug solution for 30 seconds, take them out and place them on filter paper to crawl, wait for them to dry on their own, transfer them into a sterilized finger-shaped tube, and use newly germinated wheat seeds as food. The treated test insects are placed in an artificial intelligence insect breeding room under the conditions of a temperature of (25±1)℃, a relative humidity of 70%±10%, and a photoperiod of 16h:8h. Set up 4 replicates for each concentration, with 20 larvae in each replicate. Check the death of the test insects after 4 days, touch the back of the larvae with tweezers, and the insect body is obviously unable to curl up and crawl, which is dead.

[0161] 3. Method for determining the toxicity of golden needle worm: Place the test worm in a funnel that has been padded with filter paper in advance, use a pipette to transfer 3 mL of the diluted drug solution to the funnel, let the test worm remain in the drug solution for 30 seconds, then use filter paper to absorb the excess drug solution, place the test worm in a sterilized glass insect tube with a diameter of 1.8 cm and a height of 8 cm, and add an appropriate amount of wet soil (water content is about 18%). Use newly germinated wheat seeds to feed the test worms soaked in the drug, and plug the insect tube to prevent the test worms from escaping. Set up 4 replicates for each concentration, with 20 larvae in each replicate. Rearing conditions: temperature (20±1)℃, relative humidity of 50%~60%, no light. Check the death of the test worms 3 days after treatment. The worm body is obviously shrunk or cannot crawl normally when touched with a pen tip, which is considered dead.

[0162] According to the survey data of the above tests, the adjusted mortality rate of each treatment is calculated according to the following formula, and the calculation results are retained to two decimal places:

[0163]

[0164] Where:

[0165] P——mortality rate, in percentage (%);

[0166] K——indicates the number of dead insects, in units of heads;

[0167] N——represents the total number of insects treated, in heads.

[0168]

[0169] Where:

[0170] P1——adjusted mortality rate, in percentage (%);

[0171] P t ——Treatment mortality rate, expressed as percentage (%);

[0172] P0——blank control mortality rate, in percentage (%).

[0173] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction is performed according to the above formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0174] Analyze with statistical analysis system to find out the toxicity regression equation, LC 50 Value, to evaluate the activity of the test agent on the biological test material.

[0175] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0176]

[0177] Where:

[0178] ATI——Actual Toxicity Index of Mixture;

[0179] S——LC of standard reagent 50 , the unit is milligrams per liter (mg / L);

[0180] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0181] TTI=TI A ×P A +TI B ×P B

[0182] Where:

[0183] TTI – Theoretical Toxicity Index of Mixtures;

[0184] TI A ——A: toxicity index of the agent;

[0185] P A ——The percentage of agent A in the mixture, in percentage (%);

[0186] TI B ——Toxicity index of agent B;

[0187] P B ——The percentage of agent B in the mixture, in percentage (%).

[0188]

[0189] Where:

[0190] CTC – Co-toxicity coefficient;

[0191] ATI——Actual Toxicity Index of Mixture;

[0192] TTI - Theoretical Toxicity Index of Mixture.

[0193] The test results are shown in the following table:

[0194] Table 9 Results of the indoor combined toxicity test of the mixture of fluopicolide + metalaxyl-M and its mixture with fluopicolide against A. corydalis

[0195]

[0196]

[0197] Note: The mixture of fluopicolide + metalaxyl-M had no significant insecticidal activity against A. corydalis at the designed maximum concentration of 200 mg / L, and the EC could not be calculated. 50 and virulence regression equations.

[0198] The test results show (see Table 9) that the mixture of fluopicolide and metalaxyl-M has no obvious indoor activity against the copper green beetle, and flufenac has good activity against the copper green beetle; the mixture of fluopicolide and metalaxyl-M is formulated with flufenac to form a compound agent, which can significantly increase the toxicity of flufenac and enhance the control effect on target pests.

[0199] Table 10 Results of indoor combined toxicity test of the mixture of “flufenacil + metalaxyl-M” and its mixture with flufenacil against cutworms

[0200] Test drug Virulence regression equation <![CDATA[LC 50 (mg.L -1 )]]> Co-toxicity coefficient Fluoxathiapiprolin:Metalaxyl-M (A:B=3:2) / / / Fluoxathiapiprolin:Metalaxyl-M (A:B=2:2) / / / Flufenac (C) y=3.9969+1.4226x 5.0714 / A:B:C(3:2:10) y=3.8062+1.7094x 4.9931 101.568 A:B:C(3:2:15) y=3.8255+1.8183x 4.4254 114.598 A:B:C(3:2:20) y=4.1439+1.4122x 4.0384 125.579 A:B:C(3:2:25) y=4.0567+1.6646x 3.6870 137.548 A:B:C(3:2:30) y=3.9726+1.7181x 3.9626 127.982 A:B:C(3:2:35) y=3.9722+1.6762x 4.1035 123.587 A:B:C(3:2:40) y=3.8399+1.8047x 4.3935 115.430 A:B:C(5:3:10) y=3.7588+1.7668x 5.0412 100.599 A:B:C(5:3:15) y=3.9304+1.7357x 4.1327 122.714 A:B:C(5:3:20) y=4.0852+1.5193x 4.0007 126.763 A:B:C(5:3:25) y=4.0443+1.6250x 3.8737 130.919 A:B:C(5:3:30) y=3.9848+1.7555x 3.7870 133.916 A:B:C(5:3:35) y=4.0244+1.5597x 4.2218 120.124 A:B:C(5:3:40) y=3.8621+1.6927x 4.7016 107.865

[0201] Note: The mixture of fluopicolide + metalaxyl-M had no significant insecticidal activity against cutworms at the designed maximum concentration of 200 mg / L, and the EC 50 and virulence regression equations.

[0202] The test results show (see Table 10) that the mixture of fluopicolide and metalaxyl-M has no obvious indoor activity against small cutworms, while flufenac has good activity against small cutworms; the mixture of fluopicolide and metalaxyl-M and flufenac can significantly increase the toxicity of flufenac and enhance the control effect against small cutworms.

[0203] Table 11 Results of the indoor combined toxicity test of the mixture of fluopicolide + metalaxyl-M and its mixture with fluopicolide against wireworms

[0204]

[0205]

[0206] Note: The mixture of fluopicolide + metalaxyl-M had no significant insecticidal activity against wireworms at the designed maximum concentration of 200 mg / L, and the EC 50 and virulence regression equations.

[0207] The test results show (see Table 11) that the mixture of fluopicolide and metalaxyl-M has no obvious indoor activity against wireworms, while flufenac has good activity against wireworms. The mixture of fluopicolide and metalaxyl-M and flufenac can significantly increase the toxicity of flufenac and enhance the control effect against wireworms.

[0208] Example 3: Field efficacy test for controlling corn stalk rot and underground pests

[0209] The test site is a corn field in Gu Lao Village, Wucheng Town, Wuyang County, Henan Province. The test site is sandy loam with good fertility. The farming conditions of all test plots are relatively uniform, the irrigation conditions are good, and the previous crop is wheat.

[0210] Test targets: underground pests (mainly small cutworms), corn stalk rot (the pathogens were isolated and identified as Pythium aphanidermatum and Fusarium moniliforme).

[0211] Experimental crops: corn (Yinhai 568).

[0212] Experimental design: The experiment has 11 treatments, 4 replications, randomized block arrangement, and a plot area of ​​20m 2 The treatments and dosage of active ingredients are shown in the table below. The water treatment was used as a blank control, and the interval between cells was 1m as a protective row. Protective rows were set up around the test site. Seed dressing was carried out one day before sowing. All the treatments were mixed with an appropriate amount of water, and then sown after drying.

[0213] Table 12 Treatments and dosage of active ingredients

[0214]

[0215]

[0216] Seedling emergence rate investigation: select a small piece of land in the experimental field, select 200 corn seeds for each treatment after coating and sow them on the land, investigate the emergence time and emergence rate of each treatment (seedling emergence stage: when the first green leaf emerges from the coleoptile and the corn seedlings are 2 cm tall, they are considered to have emerged, and when the height of the whole field is more than 60%, it is the emergence stage).

[0217]

[0218] Survey on underground pest damage: Survey once half a month after seedlings are planted, randomly sample 5 points in each plot, survey 20 plants at each point, a total of 100 plants, investigate corn damage, calculate the rate of damaged plants and control effectiveness. Survey insect population density, adopt soil excavation sampling survey, sample 5 points in a "Z" shape in each plot, each point is 50cm×50cm, the excavation depth is 30cm, and the insect population per unit area is recorded in detail.

[0219]

[0220] Survey on the occurrence of stem base rot: During the adult plant stage (after the milky stage), 5 points were randomly selected in each plot, 20 corn plants were marked at each point, totaling 100 plants, to investigate the occurrence of stem base rot. The disease was graded according to the 7-level grading standard for stem base rot, and the disease index and prevention and control effect were calculated.

[0221] Corn stalk rot classification standards:

[0222] Level 0: The whole plant grows normally, with symptoms of green withering and yellowing on the middle and lower leaves, normal growth of the stem base and normal growth of the fruit clusters.

[0223] Level 1: All leaves of the plant show symptoms of wilt, the stem base grows normally, and the fruit clusters grow normally.

[0224] Level 3: All leaves of the plant show typical wilt symptoms, the base of the stem changes color and appears slightly waterlogged, and the fruit clusters are basically normal.

[0225] Level 5: All leaves of the plant show typical wilt symptoms, the base of the stem becomes noticeably soft but does not fall over, the fruit clusters droop, and the grains are not full.

[0226] Level 7: The whole plant is dead and fallen over, the vascular bundles at the base of the stem are ruptured, and the grains are shriveled.

[0227]

[0228]

[0229] Yield measurement during harvest: After the corn is fully mature, samples are taken from each plot for yield measurement, the yield of each plot is recorded, and the yield per hectare is converted.

[0230]

[0231] The results of the field efficacy test are as follows:

[0232] Table 13 Effects of different seed coating treatments on corn germination rate

[0233] deal with Seedling rate (%) Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 95.25 Preparation Example 2: 35% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+30%) 93.88 Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%) 81.75 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 91.88 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 84.63 Comparative Example 4: 33% fluopicolide·flufenac suspension seed coating agent (3%+30%) 86.63 Comparative Example 5: 32% metalaxyl-M·flufenac suspension seed coating agent (2%+30%) 92.50 Comparative Example 11: 200 g / L fluopicolide suspension seed coating agent 78.25 Comparative Example 12: 35% Metalaxyl-M suspension seed coating agent 76.13 Comparative Example 13: 22% flufenac suspension seed coating agent 79.50 Blank control 73.75

[0234] As can be seen from Table 13, the best emergence rate was 95.25% when treated with 30% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+25%), followed by 93.88% when treated with 35% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+30%), and the blank CK had a poor emergence rate of 73.75%. The use of the pesticide composition of the present invention can effectively prevent corn worms from being eaten by underground pests, prevent the invasion of pathogens, and improve the seed emergence rate.

[0235] Table 14 Control effect of different seed coating agents on underground corn pests

[0236] deal with Damaged plant rate (%) Control effect (%) Insect repellent effect (%) Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 3.00 91.31 87.14 Preparation Example 2: 35% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+30%) 3.50 89.85 84.56 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 9.75 71.69 66.23 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 9.00 73.75 66.56 Comparative Example 4: 33% fluopicolide·flufenac suspension seed coating agent (3%+30%) 7.00 79.60 73.08 Comparative Example 5: 32% metalaxyl-M·flufenac suspension seed coating agent (2%+30%) 7.50 78.10 71.57 Comparative Example 13: 22% flufenac suspension seed coating agent 10.50 69.48 64.11 Blank control 34.50 / /

[0237] From the test results in the above table, it can be seen that the application of fluopicolide + metalaxyl + flufenac seed dressing agent in effective amounts to corn seeds has a plant control effect of 91.31% and 89.85% respectively; the insect control effect is 87.14% and 84.56% respectively.

[0238] Table 15 The control effect of different seed coating agents on corn stalk rot

[0239] deal with Disease rate (%) Disease index Disease control effect (%) Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 8.25 1.89 91.36 Preparation Example 2: 35% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+30%) 8.75 2.46 88.77 Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%) 14.00 4.36 80.15 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 16.25 5.25 76.06 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 19.25 6.68 69.55 Comparative Example 4: 33% fluopicolide·flufenac suspension seed coating agent (3%+30%) 18.00 5.50 74.94 Comparative Example 5: 32% metalaxyl-M·flufenac suspension seed coating agent (2%+30%) 19.75 7.32 66.73 Comparative Example 11: 200 g / L fluopicolide suspension seed coating agent 17.25 5.68 74.35 Comparative Example 12: 35% Metalaxyl-M suspension seed coating agent 24.50 8.14 63.10 Blank control 40.75 22.04 -

[0240] The control effects of different seed coating agents on corn stalk rot are shown in the table above. In the milky stage of corn, the stalk rot diseased plant rates treated with 30% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+25%) and 35% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+30%) were 8.25% and 8.75% respectively, and the diseased plant rate of the blank control was 40.75%. The suspension seed coating agent of the present invention has an excellent control effect on corn stalk base rot.

[0241] Table 16 Effects of different seed coating agents on corn yield

[0242] deal with <![CDATA[Yield (kg / hm 2 )]]> Yield increase rate (%) Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 6115.28 17.66 Preparation Example 2: 35% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+30%) 6078.97 16.96 Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%) 5456.40 4.98 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 5542.47 6.64 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 5531.38 6.42 Comparative Example 4: 33% fluopicolide·flufenac suspension seed coating agent (3%+30%) 5778.84 11.18 Comparative Example 5: 32% metalaxyl-M·flufenac suspension seed coating agent (2%+30%) 5722.98 10.11 Comparative Example 11: 200 g / L fluopicolide suspension seed coating agent 5426.49 4.40 Comparative Example 12: 35% Metalaxyl-M suspension seed coating agent 5359.89 3.12 Comparative Example 13: 22% flufenac suspension seed coating agent 5374.87 3.41 Blank control 5197.60 /

[0243] Compared with the blank control, various seed coating treatments increased corn grain yield, with the increase rate ranging from 3.12% to 17.66%. Among them, the corn yield treated with 30% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+25%) and 35% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+30%) was the highest.

[0244] Example 4: Field efficacy test on wheat root rot and wireworm

[0245] The test site was conducted in the wheat fields of Beigaomen Village, Dongwang Township, Quyang County, Baoding City, Hebei Province. The test plots were flat, with medium to high fertility, and the soil was carbonate brown soil. The management level of all test plots was consistent, and root rot and wireworms occurred all year round in the test plots, meeting the conditions required for the test.

[0246] Experimental crops: wheat (Shixin 828).

[0247] Test targets: wheat root rot (Bipolaris sorokiniana), wireworm (Pleonomuscanaliculatus).

[0248] Experimental design: There are 11 treatments in the experiment, each treatment is repeated 4 times, and all experimental plots are arranged in random blocks. The treatments and active ingredient dosages are shown in the table below.

[0249] Table 17 Treatments and dosage of active ingredients

[0250] Serial number deal with Dosage of active ingredient (g / 100kg seeds) 1 Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 150 2 Preparation Example 2: 35% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+30%) 150 3 Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%) 150 4 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 150 5 Comparative Example 3: 27% metalaxyl-M·flufenac suspension seed coating agent (2%+25%) 150 6 Comparative Example 4: 33% fluopicolide·flufenac suspension seed coating agent (3%+30%) 150 7 Comparative Example 5: 32% metalaxyl-M·flufenac suspension seed coating agent (2%+30%) 150 8 Comparative Example 11: 200 g / L fluopicolide suspension seed coating agent 15 9 Comparative Example 12: 35% Metalaxyl-M suspension seed coating agent 18 10 Comparative Example 13: 22% flufenac suspension seed coating agent 250 11 Blank control /

[0251] Test method: Seeds will be coated and treated once on October 3, 2023, sown on October 5, germination rate survey conducted on October 20, pest survey conducted on November 12, disease survey conducted on May 4, 2024, actual yield measured on June 16, and the control effect calculated.

[0252] Determination of wheat germination rate: 200 seeds were sown in a fixed area in each plot. After all the wheat seedlings had emerged, the number of seedlings was recorded and the germination rate was calculated.

[0253] Wheat root rot prevention and control survey: Sampling survey was conducted at 5 points in each plot, 20 plants were surveyed at each point, and the number of diseased plants at each level was recorded.

[0254] Root rot disease classification is as follows.

[0255] Level 0: There are no lesions at the base of the plant stem and the main root.

[0256] Level 1: There are a few spots at the stem base and taproot.

[0257] Level 3: There are more lesions at the stem base and main root, and the lesion area accounts for 25% to 50% of the total area of ​​the stem base and roots.

[0258] Level 5: There are more and larger lesions at the stem base and main root, and the lesion area accounts for 50% to 75% of the total area of ​​the stem base and roots.

[0259] Level 7: The spots on the stem base or main root are connected, forming a phenomenon surrounding the stem, but the root system is not dead.

[0260] Level 9: Root necrosis and above-ground parts of diseased plants wilt or die.

[0261] The calculation method for the root rot efficacy is as follows:

[0262]

[0263] Wireworm control survey: Sampling was conducted at 5 points in a "Z" shape in each plot, with each point having a ridge length of 1m. The total number of plants in the survey point and the number of plants damaged by wireworms were investigated.

[0264] Method for calculating the efficacy of Wireworm:

[0265]

[0266] Actual yield measurement: Actual yield measurement is carried out during the wheat harvest period.

[0267]

[0268] The results of the field test are shown in the following table:

[0269] Table 18 Effects of different seed coating treatments on wheat germination rate

[0270] deal with Seedling rate (%) Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 91.00 Preparation Example 2: 35% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+30%) 89.25 Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%) 73.25 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 79.75 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 77.75 Comparative Example 4: 33% fluopicolide·flufenac suspension seed coating agent (3%+30%) 82.38 Comparative Example 5: 32% metalaxyl-M·flufenac suspension seed coating agent (2%+30%) 79.38 Comparative Example 11: 200 g / L fluopicolide suspension seed coating agent 78.25 Comparative Example 12: 35% Metalaxyl-M suspension seed coating agent 75.38 Comparative Example 13: 22% flufenac suspension seed coating agent 74.38 Blank control 73.00

[0271] As can be seen from Table 18, each treatment has a significant difference in the emergence of small seedlings. The germination rate of 91.00% treated with 30% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+25%) was the best, followed by 89.25% treated with 35% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+30%), and the blank CK had a poor germination rate of 73.00%.

[0272] Table 19 Effects of different seed coating agents on the prevention and control of wheat root rot

[0273]

[0274]

[0275] The control effect of each test agent on wheat root rot was 61.39% to 89.07%. The combination of fluopicolide + metalaxyl-M + flufenac can significantly improve the control effect on wheat root rot.

[0276] Table 20 The control effect of different seed dressing agents on wireworms

[0277] deal with Damaged plant rate (%) Control effect (%) Insect repellent effect (%) Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 4.00 86.01 90.04 Preparation Example 2: 35% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+30%) 4.75 83.33 85.51 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 8.00 72.03 75.34 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 8.25 71.10 73.30 Comparative Example 4: 33% fluopicolide·flufenac suspension seed coating agent (3%+30%) 6.75 76.36 77.13 Comparative Example 5: 32% metalaxyl-M·flufenac suspension seed coating agent (2%+30%) 7.00 75.40 79.03 Comparative Example 13: 22% flufenac suspension seed coating agent 7.50 73.67 75.40 Blank control 28.50 / /

[0278] From the test results in the above table, it can be seen that the application of fluopicolide + metalaxyl + flufenac seed dressing agent to wheat seeds according to the effective amount set in the test has a plant control effect of 86.01% and 83.33% respectively; the insect control effect is 90.04% and 85.51% respectively.

[0279] Table 21 Effects of different seed coating treatments on wheat yield

[0280] deal with <![CDATA[Yield (kg / hm 2 )]]> Yield increase rate (%) Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 7533.36 10.03 Preparation Example 2: 35% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+30%) 7470.55 9.11 Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%) 7330.89 7.07 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 7183.04 4.91 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 7167.09 4.68 Comparative Example 4: 33% fluopicolide·flufenac suspension seed coating agent (3%+30%) 7368.63 7.62 Comparative Example 5: 32% metalaxyl-M·flufenac suspension seed coating agent (2%+30%) 7260.67 6.04 Comparative Example 11: 200 g / L fluopicolide suspension seed coating agent 7276.25 6.27 Comparative Example 12: 35% Metalaxyl-M suspension seed coating agent 6989.97 2.09 Comparative Example 13: 22% flufenac suspension seed coating agent 7116.41 3.94 Blank control 6846.95 /

[0281] Compared with the blank control, the actual yield increase rates of each test agent treatment were 2.09% to 10.03%. Among them, the actual yield increase rates of 30% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+25%) and 35% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+30%) were 10.03% and 9.11% respectively compared with the blank control, which had a certain yield-increasing effect on wheat production.

[0282] Example 5: Field efficacy test on wheat stem rot

[0283] The test site is located in the wheat field of Liuxian Village, Taozhuang Village, Baodian Town, Ningjin County, Dezhou City, Shandong Province. The previous crop was corn, and wheat was planted all year round. In recent years, the wheat stem rot has been serious in this plot, and the diseased plants are evenly distributed. The soil of the test site is sandy loam with high nutrient content, flat terrain, basically complete farmland water conservancy facilities, and good irrigation and drainage ditch conditions.

[0284] Wheat variety tested: Jimai 22.

[0285] Experimental target: The strains isolated and identified indoors were mainly Fusarium graminearum and Pseudocerillus graminearum.

[0286] Application time and method: Wheat is coated according to the designed dosage of the experiment one day before sowing. The pesticide is diluted with water, then sprayed on the wheat seeds and mixed well, then dried and sown. The wheat sowing time is October 23, 2023.

[0287] Experimental design: There are 11 treatments in total, each with 4 replicates, and each plot is 50m 2 All experimental plots were arranged in random blocks. The treatments and dosages of active ingredients are shown in the table below.

[0288] Table 22 Treatments and dosage of active ingredients

[0289] Serial number deal with Dosage of active ingredient (g / 100kg seeds) 1 Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 150 2 Preparation Example 3: 33% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+25%) 150 3 Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%) 150 4 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 150 5 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 150 6 Comparative Example 6: 8% fluazifop-butyl·metalaxyl-M suspension seed coating agent (5%+3%) 150 7 Comparative Example 7: 28% metalaxyl-M·flufenac suspension seed coating agent (3%+25%) 150 8 Comparative Example 8: 30% fluopicolide·flufenac suspension seed coating agent (5%+25%) 15 9 Comparative Example 11: 200 g / L fluopicolide suspension seed coating agent 18 10 Comparative Example 12: 35% Metalaxyl-M suspension seed coating agent 250 11 Blank control /

[0290] Test survey method:

[0291] Conduct safety investigations during the wheat greening period, observe plant height, leaf color, etc., and check the effects of seed dressing agents on the growth and development of wheat seedlings.

[0292] The survey was conducted during the milky stage of wheat. A 5-point sampling method was adopted in each plot, with 20 plants surveyed at each point, totaling 100 plants. The number of diseased plants at each level was investigated, and the disease index and prevention and control effect were calculated.

[0293] The condition is graded according to the following criteria:

[0294] Level 0: No browning symptoms on the whole plant;

[0295] Level 1: The roots are browning;

[0296] Level 3: The first node of the aboveground part turns brown and rots;

[0297] Level 5: The second node of the aboveground part turns brown and rots;

[0298] Level 7: The lesions extend beyond the second stem node, but there is no white spike;

[0299] Level 9: The lesions extend beyond the second stem node and there are white spikes;

[0300] Disease index and prevention and treatment effect calculation formula:

[0301]

[0302]

[0303] The results of the field test are shown in the following table:

[0304] Table 23 The control effect of different seed coating agents on wheat stem rot

[0305] deal with Disease index Control effect (%) Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 1.78 90.59 Preparation Example 3: 33% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+25%) 2.64 86.07 Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%) 3.89 79.50 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 5.03 73.25 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 8.14 56.99 Comparative Example 6: 8% fluazifop-butyl·metalaxyl-M suspension seed coating agent (5%+3%) 4.33 77.03 Comparative Example 7: 28% metalaxyl-M·flufenac suspension seed coating agent (3%+25%) 8.11 57.02 Comparative Example 8: 30% fluopicolide·flufenac suspension seed coating agent (5%+25%) 5.17 72.77 Comparative Example 11: 200 g / L fluopicolide suspension seed coating agent 5.28 72.20 Comparative Example 12: 35% Metalaxyl-M suspension seed coating agent 8.89 53.06 Blank control 18.97 -

[0306] During the milky stage of wheat, the disease index of the treatment with 30% fluopicolide·metalaxyl·flufenac suspension seed dressing agent (3%+2%+25%) was 1.78%, and the prevention efficiency was 90.59%, which was the best prevention efficiency; the disease index of the treatment with 33% fluopicolide·metalaxyl·flufenac suspension seed dressing agent (5%+3%+25%) was 2.64%, and the prevention efficiency was 86.07%, which was the second best prevention efficiency.

[0307] Example 6: Field efficacy test on peanut root rot and white grubs

[0308] Test location: The test was conducted in the peanut field of Nansongjia Village, Nanshu Town, Laixi City, Qingdao City, Shandong Province. The previous crop of the test field was corn. The test field has flat terrain and medium to high soil fertility. Wheat, corn and peanuts are planted in rotation all year round. Peanut root rot occurs moderately and white grubs occur heavily.

[0309] Experimental crop: peanut (Huayu No. 22).

[0310] Test targets: Peanut root rot, white grubs.

[0311] Experimental design: The experimental facility has 15 treatments, each treatment is replicated 4 times, arranged in random blocks, and the plot area is 25m 2 . One day before sowing peanuts, accurately absorb the pesticide according to the experimental design dosage, add a small amount of water, shake well, then put the seeds into a plastic bag, shake and mix until mixed well, and dry. The treatments and active ingredient dosages are shown in the following table.

[0312] Table 24 Treatments and dosage of active ingredients

[0313]

[0314]

[0315] Test survey method:

[0316] Peanut root rot survey: A survey was conducted once during the peanut harvest period. Five random sampling points were selected in each plot, with 4 plants selected at each point. The number of plants affected by peanut root rot and the disease index of each treatment were investigated.

[0317] Peanut root rot classification standards:

[0318] Grade 0, no lesions at the stem base and taproot;

[0319] Level 1, a few lesions on the stem base and taproot;

[0320] Level 3: There are many lesions on the stem base and main root, and the lesion area accounts for 25% to 50% of the total area of ​​the stem and root;

[0321] Level 5: There are many and larger lesions on the stem base and main root, and the lesion area accounts for 50% to 75% of the total area of ​​the stem and root;

[0322] Level 7: The lesions on the stem base and main root are connected, forming a ring stem phenomenon, but the root system is not dead;

[0323] Level 9: root necrosis and aboveground part of the plant wilts or dies.

[0324]

[0325] Survey of peanut field grubs. Survey once during the peanut harvest period (August 30). During the harvest, a "Z"-shaped five-point sampling method was used in each plot, with 4 mounds taken at each point and a soil digging depth of 30 cm. The number of surviving grubs and the number of worm-eaten fruits in the soil samples were investigated, and the pest control effect and pod control effect were calculated.

[0326] Grading standards for insect-infested fruits:

[0327] Level 0: Pods are intact and not damaged;

[0328] Level 1: There are signs of damage on the pod skin;

[0329] Level 2: The pods have small holes, but the kernels are intact and the yield is not affected;

[0330] Level 3: There are large holes in the pods and 1 / 2 of the kernels are damaged, affecting the yield;

[0331] Level 4: Pods and kernels are damaged > 1 / 2.

[0332]

[0333]

[0334] Pod yield in each plot: All pods in each plot are harvested and dried to calculate the yield.

[0335]

[0336] The results of the field efficacy test are as follows:

[0337] Table 24 The control effect of different seed coating agents on peanut root rot

[0338] deal with Disease index Control effect (%) Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 2.25 83.11 Preparation Example 3: 33% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+25%) 1.86 86.48 Preparation Example 4: 38% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+30%) 1.97 85.60 Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%) 3.31 76.39 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 3.69 72.32 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 5.81 57.87 Comparative Example 6: 8% fluazifop-butyl·metalaxyl-M suspension seed coating agent (5%+3%) 2.97 78.88 Comparative Example 7: 28% metalaxyl-M·flufenac suspension seed coating agent (3%+25%) 6.00 56.27 Comparative Example 8: 30% fluopicolide·flufenac suspension seed coating agent (5%+25%) 3.97 71.60 Comparative Example 9: 35% fluopicolide·flufenac suspension seed coating agent (5%+30%) 4.44 67.70 Comparative Example 10: 33% metalaxyl-flufenacil suspension seed coating agent (3% + 30%) 5.89 57.46 Comparative Example 11: 200 g / L fluopicolide suspension seed coating agent 4.25 69.08 Comparative Example 12: 35% Metalaxyl-M suspension seed coating agent 6.33 54.07 Blank control 13.92 -

[0339] When investigating the occurrence of root and stem rot during the peanut harvest period, it was found that root rot occurred in all treatment areas. Among them, the treatment with 33% fluopicolide·metalaxyl·flufenac suspension seed coating agent (5%+3%+25%) had the lowest disease index and the best prevention effect, with a prevention effect of 86.48% against root and stem rot; followed by the treatment with 38% fluopicolide·metalaxyl·flufenac suspension seed coating agent (5%+3%+30%) and the treatment with 30% fluopicolide·metalaxyl·flufenac suspension seed coating agent (3%+2%+25%), with prevention effects of 85.60% and 83.11% against root and stem rot, respectively.

[0340] Table 25 The control effect of different seed coating agents on peanut grubs

[0341]

[0342]

[0343] Before harvest, the control effect of each treatment on underground pests of peanuts was investigated. The underground pests were mainly white grubs, and no wireworms were found. The results showed that the best peanut fruit preservation effect was achieved by 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) and 33% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+25%), which were 89.18% and 86.55% respectively, significantly higher than other treatments. The best pest control effect was achieved by 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) and 33% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+25%), which were 87.96% and 85.40% respectively.

[0344] Table 26 Effects of different seed coating treatments on peanut yield

[0345] deal with <![CDATA[Yield (kg / hm 2 )]]> Yield increase rate (%) Preparation Example 1: 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) 3605.37 33.15 Preparation Example 3: 33% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+25%) 3542.20 30.81 Preparation Example 4: 38% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+30%) 3483.36 28.64 Comparative Example 1: 5% fluazifop-butyl·metalaxyl-M suspension seed coating agent (3%+2%) 3212.50 18.64 Comparative Example 2: 28% fluopicolide·flufenac suspension seed coating agent (3%+25%) 3342.22 23.43 Comparative Example 3: 27% metalaxyl-flufenacil suspension seed coating agent (2% + 25%) 3131.23 15.64 Comparative Example 6: 8% fluazifop-butyl·metalaxyl-M suspension seed coating agent (5%+3%) 3232.12 19.36 Comparative Example 7: 28% metalaxyl-M·flufenac suspension seed coating agent (3%+25%) 3109.29 14.83 Comparative Example 8: 30% fluopicolide·flufenac suspension seed coating agent (5%+25%) 3380.38 24.84 Comparative Example 9: 35% fluopicolide·flufenac suspension seed coating agent (5%+30%) 3358.97 24.05 Comparative Example 10: 33% metalaxyl-flufenacil suspension seed coating agent (3% + 30%) 3098.29 14.42 Comparative Example 11: 200 g / L fluopicolide suspension seed coating agent 3073.26 13.50 Comparative Example 12: 35% Metalaxyl-M suspension seed coating agent 2826.48 4.38 Comparative Example 13: 22% flufenac suspension seed coating agent 2936.23 8.44 Blank control 2707.81 /

[0346] The yield statistics of peanut pods after harvesting and drying showed that the average yield of each treatment was 2707.81-3605.37 kg / hm 2 The yield of the group treated with 30% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (3%+2%+25%) was the highest at 3605.37 kg / hm 2 , the yield increased by 897.56kg / hm compared with the blank control 2 , with an increase of 33.15%; the group treated with 33% fluopicolide·metalaxyl-M·flufenac suspension seed coating agent (5%+3%+25%) was second, with a yield of 3542.20kg / hm 2 , an increase of 30.81% compared with the blank control.

[0347] Overall, the seed coating treatment with a combination of fluazifop-butyl + metalaxyl + fluazifop-butyl has a good control effect on peanut root rot and peanut grubs, and can comprehensively prevent and control peanut soil-borne diseases and underground pests.

[0348] Through indoor toxicity determination and field tests, the reasonable compounding of fluopicolide + metalaxyl + flufenicillin of the present invention shows good control effect on peanut, corn, wheat soil-borne diseases and underground pests. The compounded composition or its preparation obtained by the present invention has significant control effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging drug retention. It also includes a seed treatment suspension of active ingredients fluopicolide + metalaxyl + flufenicillin, which makes the seed coating film fast and not threshed, and is safe for crop seeds and has good tolerance. In addition, no compound agent was found to cause phytotoxicity to crops in the test, indicating that when the bactericidal synergistic synergy of the obtained pesticide composition or preparation is improved, the production cost and use cost can be reduced, and it is safe for crops.

[0349] Although the present invention has been described in detail above with general instructions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition containing pentothiopyrad and metalaxyl-M, characterized in that: The pesticide composition comprises a fungicide and an insecticide, wherein the fungicide is fluopicolide and metalaxyl-M, and the insecticide is fluopicolide.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of pentocyclamide to metalaxyl-M in the fungicide is 7:1 to 1:

7.

3. The pesticide composition according to claim 2, characterized in that The mass ratio of fluopicolide to metalaxyl-M in the fungicide is 6:1 to 1:

5.

4. The pesticide composition according to claim 1, characterized in that The mass ratio of the bactericide to the insecticide is 4:5 to 1:

20.

5. The pesticide composition according to claim 4, characterized in that The mass ratio of the bactericide to the insecticide is 8:15 to 1:

7.

6. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition being 100 wt%, the total content of the fungicide in the pesticide composition is 0.5% to 50%, preferably 1% to 10%, and more preferably 2% to 8%.

7. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition being 100 wt%, the content of the insecticide in the pesticide composition is 5% to 50%, preferably 10% to 40%, and more preferably 15% to 35%.

8. The pesticide composition according to claim 1, characterized in that The pesticide composition also includes an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a warning color, a film former, a synergist and a carrier.

9. The pesticide composition according to claim 7, characterized in that: The formulation of the pesticide composition is a seed treatment suspension, a microcapsule suspension or a dry seed dressing; preferably, the formulation of the pesticide composition is a seed treatment suspension.

10. Use of the pesticide composition according to any one of claims 1 to 9 in preventing and controlling plant diseases and / or insect pests, characterized in that: The plants include corn, wheat or peanuts; The plant diseases are corn stem rot, wheat root rot, wheat stem rot, and peanut root rot; The insect pests are white grubs, cutworms, wireworms, fall armyworms and corn borers.

Images