A probiotic-containing composition, its preparation method and applications

Abstract

The present invention belongs to the technical field of the preparation of probiotics and raw materials of medicine and food homology, and specifically relates to a probiotic-containing composition, a preparation method thereof, and an application. The raw materials of the probiotic-containing composition include: 3-20 parts of hawthorn, 0.1-2 parts of chicken gizzard-membrane, 0.3-3 parts of Poria cocos, 0.5-5 parts of malt, 0.2-2 parts of Lactobacillus rhamnosus GG, 0.2-2 parts of Bifidobacterium animalis subsp. lactis HN019, 0.02-1 part of Lactobacillus fermentum CECT5716, and 0.02-1 part of Lactobacillus rhamnosus HN001. The solid composition based on probiotics provided by the present invention has the effects of regulating gastrointestinal function, moistening the intestines and relieving constipation, and enhancing immunity. Moreover, it can form a delicate, particle-free and relatively uniform suspension by simply brewing with warm water. The composition is not easy to agglomerate during transportation and storage and can maintain a good viable count of bacteria, with good application value.

Description

Technical Field

[0001] The present invention belongs to the technical field of preparation of probiotics and raw materials homologous to medicine and food, and particularly relates to a composition containing probiotics, a preparation method thereof and an application thereof. Background Art

[0002] Bad eating habits and lifestyles, such as excessive stress, abuse of antibiotics, frequent drinking, smoking, frequent eating at night, and preference for unhealthy foods such as barbecue and hot pot, will all disrupt the balance of the intestinal flora, and thus have a profound and serious negative impact on intestinal health. For example, long-term drinking not only directly damages the liver, but may also lead to the occurrence of alcoholic liver, hepatitis and even cirrhosis. After the liver is damaged, the body's detoxification function and immunity will also be weakened, making people more susceptible to diseases such as inflammation.

[0003] Probiotics are active microorganisms that change the composition of the flora in a certain part of the host in the human body and are beneficial to the host. By regulating the mucosal and systemic immune functions of the host or by regulating the balance of the intestinal flora, they play a role in promoting nutrient absorption and maintaining intestinal health, thereby producing a single microorganism or a well-defined mixed microorganism that is beneficial to health. Probiotics change the interaction of microorganisms in the intestine through their adhesiveness in the intestine and competitive inhibition with harmful bacteria, improve digestion and absorption and the intestinal barrier function, and provide protection for the health of the body.

[0004] At present, probiotic preparations are generally made into powders and tablets, and are often taken together with food during use. However, there are currently technical problems such as large loss of the number of bacteria, inconvenient storage, and serious attenuation during transportation, resulting in a reduction in their health care activity. Summary of the Invention

[0005] Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a composition containing probiotics, a preparation method thereof and an application thereof. Specifically, the solid composition based on probiotics provided by the present invention has the effects of regulating gastrointestinal function, moistening the intestines and relieving constipation, and enhancing immunity. At the same time, the composition containing probiotics does not need to add auxiliary components such as foaming agents, and can form a delicate, particle-free and relatively uniform suspension only by flushing with warm water. Moreover, the solid composition is not easy to agglomerate during transportation and storage and can maintain a good number of viable bacteria. Based on the above research results, the present invention is completed.

[0006] Specifically, the technical solution of the present invention is as follows:

[0007] The first aspect of the present invention provides a composition containing probiotics, wherein the raw materials include 3-20 parts of hawthorn, 0.1-2 parts of chicken gizzard lining, 0.3-3 parts of poria, 0.5-5 parts of malt, 0.2-2 parts of Lactobacillus rhamnosus GG, 0.2-2 parts of Bifidobacterium animalis lactis subsp. HN019, 0.02-1 parts of Lactobacillus fermentum CECT5716, and 0.02-1 parts of Lactobacillus rhamnosus HN001.

[0008] The present invention achieves a synergistic effect between the probiotic supplement and the Chinese medicinal materials through the compatibility of probiotics, hawthorn, chicken gizzard lining, Poria cocos and malt, which can not only exert the pharmacological activity of a single probiotic, Chinese medicinal material, etc., but also exert the efficacy between the probiotics and the Chinese medicinal materials.

[0009] According to the present invention, preferably, the probiotics are live bacteria, and the total live bacteria count is not less than 10 billion cfu / g.

[0010] The second aspect of the present invention provides a method for preparing the composition containing probiotics according to the first aspect, comprising: crushing and mixing hawthorn, chicken gizzard lining, poria cocos and malt respectively to obtain a mixed raw material;

[0011] Add probiotics to the mixed raw materials, mix well and package.

[0012] Furthermore, the probiotic composition can be obtained by subpackaging. In a specific embodiment of the present invention, each bag of the probiotic composition contains 0.5-5 g, and further contains 1 g. The probiotic composition is a loose powder with a white main body and a red doped part.

[0013] The probiotics are in the form of solid powder.

[0014] The hawthorn and chicken gizzard lining are sieved with 80-100 meshes, the poria and malt are sieved with 120-150 meshes, and the solid probiotic powder is sieved with 50-70 meshes. The present invention has found through research that by controlling the dosage ratio and particle size of the above-mentioned medicinal and edible raw materials and probiotic powder, it is helpful to improve its storage stability, so that the solid composition is not easy to agglomerate, and it is also helpful to form a fine, non-granular, more evenly dispersed and better-tasting suspension when re-dissolving with water.

[0015] The third aspect of the present invention provides the use of the probiotic-containing composition described in the first aspect in the preparation of products for regulating gastrointestinal function, moistening the intestines and promoting bowel movements, and improving immunity.

[0016] Beneficial technical effects of one or more of the above technical solutions:

[0017] 1. In the probiotic-containing composition provided by the above technical solution, the probiotics and the raw materials of medicine and food homology are effectively compounded, which has the effects of relieving intestinal inflammation and regulating gastrointestinal function, can relieve constipation caused by gastrointestinal dysfunction, and at the same time, can also enhance the immune activity of the body and has the ability of immune regulation.

[0018] 2. The probiotic-containing composition provided by the above technical solution can regulate the stomach and intestines, moisten the intestines and relieve constipation through the combination of specific raw materials of medicine and food homology and probiotics. The viable count ratio of Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. lactis HN019, Lactobacillus fermentum CECT5716, and Lactobacillus rhamnosus HN001 is 50:50:1:1, which is beneficial to colonize in the intestine, inhibit harmful bacteria, promote the secretion of beneficial bacteria, and enhance intestinal immunity; it can also effectively regulate the activity of macrophages in the immune system, thereby significantly enhancing human immunity.

[0019] 3. In the probiotic-containing composition provided by the above technical solution, the types and contents of probiotics are moderate, which can achieve good regulation of gastrointestinal function and immune regulation effects, and can effectively moisten the intestines and relieve constipation.

[0020] 4. The probiotic-containing composition provided by the above technical solution can form a delicate, particle-free and uniformly dispersed suspension without adding foaming aids during the preparation process, and has a good taste.

[0021] 5. The probiotic-containing composition provided by the above technical solution is not easy to agglomerate during transportation and storage, can be stored for a long time, and has good market application value.

[0022] 6. The probiotic-containing composition provided by the above technical solution can regulate the intestinal flora and can be widely applied to functional health foods such as gastrointestinal function regulation, immune regulation, and intestinal moistening and defecation. Detailed Embodiments

[0023] It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs.

[0024] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0025] A typical embodiment of the present invention provides a probiotic-containing composition, which comprises raw materials in the following parts by mass: 3-20 parts of hawthorn, 0.1-2 parts of chicken gizzard-membrane, 0.3-3 parts of poria cocos, 0.5-5 parts of malt, 0.2-2 parts of Lactobacillus rhamnosus GG, 0.2-2 parts of Bifidobacterium animalis subsp. lactis HN019, 0.02-1 part of Lactobacillus fermentum CECT5716, and 0.02-1 part of Lactobacillus rhamnosus HN001.

[0026] Through the compatibility among probiotics, hawthorn, chicken gizzard-membrane, poria cocos, and malt, the present invention plays a synergistic role between probiotic supplements and traditional Chinese medicines, which can not only exert the pharmacological activities of single probiotics, traditional Chinese medicines, etc., but also exert the pharmacodynamic effects between probiotics and traditional Chinese medicines.

[0027] Hawthorn can invigorate the spleen and promote digestion, promote blood circulation to remove blood stasis, reduce turbidity and lower lipids, and regulate the intestinal flora; chicken gizzard-membrane can strengthen the spleen and stomach, promote digestion and resolve phlegm, stimulate the gastric mucosa, promote gastric juice secretion, and aid digestion; poria cocos has the effects of promoting diuresis to eliminate dampness, tonifying the middle-jiao and strengthening the spleen, promoting gastrointestinal juice secretion, antibacterial, anti-ulcer, and enhancing immunity, etc.; malt has the effects of promoting qi circulation to relieve food stagnation, and invigorating the spleen and promoting appetite.

[0028] Lactobacillus rhamnosus GG can improve immune function, regulate intestinal health, protect the gastric mucosa, promote microecological balance, etc., resist the invasion of intestinal pathogens, and has strong biosafety and probiotic characteristics.

[0029] Bifidobacterium animalis subsp. lactis HN019 can regulate the immune system, maintain mucosal homeostasis, have good impedance effects on viruses, fungi, and bacteria, and significantly enhance human immunity.

[0030] Lactobacillus fermentum CECT5716 can prevent and treat diarrhea, restore the normal structure of the digestive tract, promote intestinal mucus secretion, improve the intestinal resistance to exogenous substances, prevent pathogens from invading through damaged cells and mucosa, promote the proliferation of macrophages and lymphocytes, induce mucosal immunity, have good probiotic characteristics, inhibit the formation of intestinal endotoxin, and have a significant effect on intestinal immune regulation.

[0031] Lactobacillus rhamnosus HN001 can improve the body's immunity, reduce the contents of cholesterol and triglycerides, increase the ratio of Bifidobacterium and Lactobacillus in the intestines of patients with cholelithiasis, reduce pathogenic bacteria, regulate the types and proportions of beneficial intestinal bacteria, and play an important role in maintaining the balance between oxidation and antioxidant in the body and delaying the aging of the body;

[0032] According to the present invention, preferably, the raw materials include 10 parts of hawthorn, 1 part of chicken gizzard-membrane, 2 parts of poria cocos, 3 parts of malt, 0.5 part of Lactobacillus rhamnosus GG, 0.5 part of Bifidobacterium animalis subsp. lactis HN019, 0.05 part of Lactobacillus fermentum CECT5716, and 0.05 part of Lactobacillus rhamnosus HN001.

[0033] According to the present invention, preferably, all probiotics are live bacteria, wherein the ratio of the live bacteria count of Lactobacillus rhamnosus GG, Bifidobacterium animalis lactis subsp. HN019, Lactobacillus fermentum CECT5716 and Lactobacillus rhamnosus HN001 is 50:50:0.5-5:0.5-5 (preferably 50:50:1:1). The live bacteria count of the total probiotics is not less than 10 billion cfu / g, further 10-20 billion cfu / g.

[0034] In another specific embodiment of the present invention, a method for preparing the probiotic-containing composition is provided, comprising: crushing and mixing hawthorn, chicken gizzard lining, poria cocos and malt respectively to obtain a mixed raw material;

[0035] Add probiotics to the mixed raw materials, mix well and package.

[0036] In another embodiment of the present invention, the probiotic composition can be obtained by subpackaging. In one embodiment of the present invention, each bag of the probiotic composition is 0.5-5g, further 1g. The probiotic composition is mainly white loose powder mixed with red powder particles.

[0037] The probiotics are a mixed bacterial agent of the above-mentioned Lactobacillus rhamnosus GG, Bifidobacterium animalis subspecies lactis HN019, Lactobacillus fermentum CECT5716 and Lactobacillus rhamnosus HN001, and can be a solid powder. The sieve mesh number of the solid probiotic powder is 50-70 meshes.

[0038] The hawthorn and chicken's gizzard lining are sieved with a mesh number of 80-100, the tuckahoe and malt are sieved with a mesh number of 120-150, and the solid probiotic powder is sieved with a mesh number of 50-70. The present invention has found through research that by controlling the dosage ratio and particle size of the above-mentioned medicinal and edible raw materials and probiotic powder, it is helpful to improve its storage stability, so that the solid composition is not easy to stick together, and it is also helpful to form a fine, non-granular, more evenly dispersed and better-tasting suspension when re-dissolving with water.

[0039] The inventors found in the study that the solid composition obtained by using the medicinal and edible raw materials and probiotics of the present invention together with foaming agents, sweeteners, acidity regulators, etc. is easy to agglomerate, and the product quality and taste are poor. Therefore, the above technical effects can be achieved through the mutual cooperation between the medicinal and edible raw materials and probiotics. At the same time, through the compatibility between hawthorn, chicken gizzard, Poria cocos, and malt in the raw materials, the dissolution of each effective ingredient is promoted, and the fermentation process of specific probiotics is coordinated, which is conducive to the colonization of probiotics in the intestines, and plays a certain degree of gastrointestinal conditioning, promoting intestinal peristalsis, moistening the intestines and laxative, and regulating immunity.

[0040] In another specific embodiment of the present invention, there is provided an application of the described probiotic-containing composition in the preparation of products for regulating gastrointestinal function, moistening the intestines and relieving constipation, and enhancing immunity.

[0041] The product can be a food or a medicine. The food can be a health food, specifically a solid beverage, and no specific limitation is made here.

[0042] The present invention will be further explained and illustrated below through examples, but it does not constitute a limitation to the present invention. It should be understood that these examples are only used to illustrate the present invention and not to limit the scope of the present invention. All raw material components in the present invention are obtained through commercial channels.

[0043] Example 1

[0044] A probiotic-containing composition, the raw materials are: 10 parts of hawthorn, 1 part of chicken gizzard-membrane, 2 parts of poria cocos, 3 parts of malt, 0.5 part of Lactobacillus rhamnosus GG, 0.5 part of Bifidobacterium animalis subsp. lactis HN019, 0.05 part of Lactobacillus fermentum CECT5716, 0.05 part of Lactobacillus rhamnosus HN001; wherein, the viable count ratio of Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. lactis HN019, Lactobacillus fermentum CECT5716 to Lactobacillus rhamnosus HN001 is 50:50:1:1; the total viable count of probiotics ≥ 10 billion cfu / g.

[0045] The preparation method includes: respectively crushing and mixing hawthorn, chicken gizzard-membrane, poria cocos and malt to obtain a mixed raw material; the sieving mesh number for crushing hawthorn and chicken gizzard-membrane is 90 meshes, and the sieving mesh number for crushing poria cocos and malt is 120 meshes.

[0046] Adding probiotics to the mixed raw material, mixing and packaging. Each bag of the probiotic-containing composition is 1 g. The probiotics are a mixed solid powder bacterium agent of the above four probiotics, and the sieving mesh number for crushing the solid probiotic powder is 60 meshes.

[0047] Example 2

[0048] A probiotic-containing composition, the raw materials are: 10 parts of hawthorn, 0.5 part of chicken gizzard-membrane, 3 parts of poria cocos, 2 parts of malt, 0.5 part of Lactobacillus rhamnosus GG, 0.5 part of Bifidobacterium animalis subsp. lactis HN019, 0.05 part of Lactobacillus fermentum CECT5716, 0.05 part of Lactobacillus rhamnosus HN001; wherein, the viable count ratio of Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. lactis HN019, Lactobacillus fermentum CECT5716 to Lactobacillus rhamnosus HN001 is 50:50:1:1; the total viable count of probiotics ≥ 10 billion cfu / g.

[0049] Preparation method, comprising: crushing and mixing hawthorn, chicken gizzard, poria cocos, and malt respectively to obtain a mixed raw material; the hawthorn and chicken gizzard are crushed and sieved through 80 meshes, and the poria cocos and malt are crushed and sieved through 140 meshes.

[0050] Add probiotics to the mixed raw material, mix well and divide into portions. Each bag of the composition containing probiotics is 1 g. The probiotics are a mixed solid powder bacterium agent of the above four probiotics, and the crushed and sieved mesh number of the solid probiotic powder is 50 meshes.

[0051] Example 3

[0052] A composition containing probiotics, with raw materials: 18 parts of hawthorn, 2 parts of chicken gizzard, 1 part of poria cocos, 5 parts of malt, 0.5 part of Lactobacillus rhamnosus GG, 0.5 part of Bifidobacterium animalis subsp. lactis HN019, 0.05 part of Lactobacillus fermentum CECT5716, 0.05 part of Lactobacillus rhamnosus HN001; wherein, the viable count ratio of Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. lactis HN019, Lactobacillus fermentum CECT5716 to Lactobacillus rhamnosus HN001 is 50:50:1:1; the total viable count of probiotics ≥ 10 billion cfu / g.

[0053] Preparation method, comprising: crushing and mixing hawthorn, chicken gizzard, poria cocos, and malt respectively to obtain a mixed raw material; the hawthorn and chicken gizzard are crushed and sieved through 100 meshes, and the poria cocos and malt are crushed and sieved through 150 meshes.

[0054] Add probiotics to the mixed raw material, mix well and divide into portions. Each bag of the composition containing probiotics is 1 g. The probiotics are a mixed solid powder bacterium agent of the above four probiotics, and the crushed and sieved mesh number of the solid probiotic powder is 70 meshes.

[0055] Comparative Example 1

[0056] The preparation method is the same as that of Example 1, the only difference being that the sieving mesh number of each raw material in Example 1 is 100 meshes.

[0057] Comparative Example 2

[0058] The preparation method is the same as that of Example 1, the only difference being that the hawthorn and chicken gizzard are crushed and sieved through 120 meshes, and the sieving mesh number of the poria cocos and malt is 90 meshes.

[0059] Comparative Example 3

[0060] The preparation method is the same as that of Example 1, the only difference being that the sieving mesh number of the solid probiotic powder is 90 meshes.

[0061] Comparative Example 4

[0062] The preparation method was the same as that of Example 1, except that malt was replaced with the same amount of coix seed.

[0063] Comparative Example 5

[0064] The preparation method was the same as that of Example 1, except that hawthorn was replaced with the same amount of dried tangerine peel.

[0065] Effect verification

[0066] (1) Verification of storage stability

[0067] The samples prepared in the above examples and comparative examples were subjected to an accelerated test (temperature 35 °C, relative humidity 75 ± 5%), and the results are recorded in Table 1.

[0068] Table 1 Accelerated test results

[0069]

[0070] As can be seen from Table 1 above, the raw material selection and particle size have a great influence on the storage stability of the final solid composition, and the occurrence of caking has a chain reaction on the viable bacteria count, that is, the more serious the caking phenomenon, the greater the decline in the viable bacteria count.

[0071] (2) Evaluation of improving immune function

[0072] Thirty Kunming mice (15 males and 15 females, weighing 18 - 22 g) were divided into a control group, a model group, and an experimental group, with 10 mice randomly assigned to each group. Among them, the control group and the model group were given 0.5 mL of sterilized water by gavage once a day; the experimental group was given a product containing probiotics (50 mg dissolved in 0.5 mL of sterilized water, Example 1) solution by gavage once a day for 14 consecutive days. At the same time, the model group and the experimental group were intraperitoneally injected with cyclophosphamide (30 mg / kg, bw) on the first, third, and fifth days respectively, for a total of three times, and the control group was injected with the same volume of physiological saline. After 14 consecutive days of intervention, the mice in each group were fasted but allowed to drink water for 16 hours. The thymus and spleen were dissected, and the organ index was calculated. The organ index = [average organ weight (mg) / average body weight (g)]. The specific results are shown in Table 2 below.

[0073] Table 2 Effect of the composition on improving immune function

[0074]

[0075] As can be seen from Table 2, compared with the model group, the thymus index and spleen index of the experimental group were significantly increased, and even equal to or slightly higher than those of the control group, indicating that the above probiotic-containing composition can enhance the body immunity of mice.

[0076] (3) Evaluation of laxative function

[0077] Thirty Kunming mice (half male and half female, weighing 18 - 22 g) were divided into a control group, a model group, and an experimental group, with 10 mice randomly assigned to each group. The control group and the model group were given 0.5 mL of sterilized water by gavage once a day; the experimental group was given a product containing probiotics (50 mg dissolved in 0.5 mL of sterilized water, Example 1) solution by gavage once a day. After 14 consecutive days of intervention, the mice in each group were fasted but allowed to drink water for 16 hours.

[0078] The model group and the experimental group were given compound diphenoxylate (5 mg / kg, bw) by gavage, and the control group was given the same volume of distilled water by gavage. After 30 min, each group of mice was given gum arabic ink (containing 10% activated carbon powder and 10% activated gum arabic) by gavage. After 30 min, 5 mice were randomly selected from each group, decapitated and sacrificed, and the intestine from the pylorus to the cecum was quickly removed. The length of the intestinal tube was measured as the total length of the small intestine L0 (cm), and the length from the pylorus to the ink front was measured as the ink propulsion length L1 (cm). The calculation formula for the ink propulsion rate of the small intestine is as follows: Ink propulsion rate = L1 / L0 × 100%. The remaining 5 mice in each group started from the gavage of ink, and the time of the first black stool excretion, the number of black stools excreted in 6 h, and the total mass of the excreted feces were recorded. The specific results are shown in Table 3.

[0079] Table 3 Laxative function of the composition

[0080]

[0081] From the above results, it can be seen that the probiotic-containing composition prepared by the present invention can significantly increase the intestinal ink propulsion rate, significantly shorten the time of the first black stool excretion, and significantly increase the number of black stools excreted in 6 h and the weight of black stools excreted in 6 h. It shows that the composition of this application has a good effect of promoting intestinal peristalsis and moistening the intestines and relieving constipation.

[0082] (4) Verification in a small-scale population

[0083] Twenty participants who worked in a sedentary environment and self-reported constipation were selected. They took the probiotic-containing composition (Example 1) orally once a day, 2 g each time, and it was dissolved by adding 100 ml of warm water (35 °C). When re-dissolved with water, a suspension with uniform dispersion, fineness, no particle feeling, and good taste could be formed. After taking it for 1 month, a feedback survey was conducted on each participant, and the evaluation criteria were as follows:

[0084] Marked effect: Constipation was significantly improved, the interval time and stool quality were close to normal, or the stool was slightly dry and the defecation interval time was within 72 hours, and most of the other symptoms disappeared;

[0085] Effective: The defecation interval time was shortened by 1 day, or the dryness of the stool quality was improved, and all other symptoms improved;

[0086] Ineffective: Constipation and other symptoms were not improved.

[0087] After statistics, the number of people with significant effects in the feedback is 10, and the number of effective people is 6. That is, the total number of people with significant effects and effective is 16, accounting for 80%. It shows that the solid composition of the present invention has the effects of promoting intestinal peristalsis and moistening the intestines and relieving constipation.

[0088] Matters not covered by the present invention are well-known technologies.

[0089] The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose is to enable those skilled in the art to understand the content of the present invention and implement it accordingly, and cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims

1. Use of a probiotic-containing composition in the preparation of a product for promoting bowel movement and enhancing immunity; The above-mentioned use realizes the improvement of immunity by increasing the thymus index and spleen index; The composition is composed of raw materials in the following parts by mass: 3 - 20 parts of hawthorn, 0.1 - 2 parts of chicken gizzard-membrane, 0.3 - 3 parts of poria cocos, 0.5 - 5 parts of malt, 0.2 - 2 parts of Lactobacillus rhamnosus GG, 0.2 - 2 parts of Bifidobacterium animalis subsp. lactis HN019, 0.02 - 1 part of Lactobacillus fermentum CECT5716, 0.02 - 1 part of Lactobacillus rhamnosus HN001; The viable count ratio of Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. lactis HN019, Lactobacillus fermentum CECT5716 to Lactobacillus rhamnosus HN001 is 50:50:0.5 - 5:0.5 - 5; The total viable count of probiotics is 10 - 20 billion cfu / g; The preparation method of the probiotic-containing composition comprises the following steps: crushing and mixing hawthorn, chicken gizzard-membrane, poria cocos and malt respectively to obtain a mixed raw material; adding probiotics to the mixed raw material, mixing and packaging; The probiotics are a mixed solid powder of Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. lactis HN019, Lactobacillus fermentum CECT5716 and Lactobacillus rhamnosus HN001, and the sieve mesh number of the solid probiotic powder is 50 - 70 meshes; The sieve mesh number of hawthorn and chicken gizzard-membrane is 80 - 100 meshes; The sieve mesh number of poria cocos and malt is 120 - 150 meshes.

2. The application according to claim 1, characterized in that, The composition is composed of the following raw materials in parts by mass: 10 parts of hawthorn, 1 part of chicken gizzard-membrane, 2 parts of poria cocos, 3 parts of malt, 0.5 part of Lactobacillus rhamnosus GG, 0.5 part of Bifidobacterium animalis subsp. lactis HN019, 0.05 part of Lactobacillus fermentum CECT5716, 0.05 part of Lactobacillus rhamnosus HN001.

3. The application according to claim 1, wherein The above-mentioned product is a food or a drug.

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