Application of raptor1a gene in genetically engineering adjustment of plant insect resistance and raptor1a gene mutant and application
By knocking out the RAPTOR1A gene in tomatoes using CRISPR-Cas9 technology, the mutant Slraptor1a was obtained. This solved the problem of improving the resistance of tomatoes to tomato leafminer without affecting tomato growth and yield, and achieved the effect of significantly reducing the number of eggs laid by adult tomato leafminer and the feeding area of larvae.
CN119530289BActive Publication Date: 2026-06-09HEBEI UNIVERSITY
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HEBEI UNIVERSITY
- Filing Date
- 2024-12-12
- Publication Date
- 2026-06-09
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Figure CN119530289B_ABST
Abstract
This invention discloses the application of the RAPTOR1A gene in genetic engineering to adjust plant insect resistance, as well as RAPTOR1A gene mutants and their applications, belonging to the field of plant genetic engineering technology. This invention is the first to discover that knocking out the RAPTOR1A gene can improve plant insect resistance. A tomato RAPTOR1A (NCBI gene number: 101266988) mutant was obtained using CRISPR-Cas9 technology. Slraptor1a Knockout of RAPTOR1A, a key component of the TOR complex, did not significantly alter tomato plant height, single fruit weight, or yield per plant. However, the mutant exhibited enhanced resistance to pests. Specifically, the number of eggs laid by adult tomato leafminers on the mutant plants decreased significantly, larval development slowed, and the area of the leafminer feeding on the host tomato leaves was reduced. This provides a reference for subsequent breeding programs that improve insect resistance without affecting tomato yield and can be applied to the improvement of resistant tomato varieties.
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