All-in-one machine for selling and automatically detecting nucleic acid molecules

Abstract

The invention provides a vending and nucleic acid molecule automatic detection all-in-one machine, and belongs to the technical field of nucleic acid self-service detection, and the vending and nucleic acid molecule automatic detection all-in-one machine comprises a product vending mechanism, an operation panel and a nucleic acid detection mechanism cabinet body, the nucleic acid detection mechanism comprises a detection box, an isothermal fluorescence detector is arranged in the detection box, and a garbage can is arranged below the detection box. A customer can operate the all-in-one machine to purchase nucleic acid detection kits or drugs or other health-related products at any time according to requirements, and after the customer detects nucleic acid samples by himself, sample single tubes or sample connection tubes are put into an isothermal fluorescence detector for automatic detection, so that the detection efficiency is improved. A detection result is checked by a customer on an operation panel or is sent to a mobile phone terminal or a computer terminal or other communication terminals, so that the convenience of nucleic acid detection and the judgment accuracy of the detection result are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid self-service detection, and in particular to an all-in-one machine for selling and automatically detecting nucleic acid molecules. Background Art

[0002] Nucleic acid testing is a precise technology for detecting DNA or RNA, and is widely used in medical diagnosis, disease prevention, drug development and other fields. However, due to the high technicality, difficulty, complex equipment, high requirements for operators and high requirements for the testing environment of nucleic acid molecule testing, nucleic acid testing is not convenient to carry out at present. Nucleic acid testing can basically only be carried out in hospitals or third-party testing platforms or research laboratories, and cannot be widely promoted to various pharmacies and drugstores for nucleic acid testing. Customers can purchase nucleic acid testing kits on their own only to facilitate sampling at home. The samples after sampling still need to be sent to the hospital for laboratory testing, especially during the high incidence of respiratory infectious diseases. Nucleic acid testing will occupy a large amount of medical resources in the hospital.

[0003] However, since not all pharmacies and drugstores sell nucleic acid testing kits, and the public still needs to go to the hospital to obtain test results after nucleic acid sampling, the public's desire to purchase nucleic acid testing kits is low, the purchasing and testing process is inconvenient, and diseases cannot be detected in time, which is not conducive to the prevention, diagnosis and treatment of diseases, especially respiratory infectious diseases that need to be detected and diagnosed quickly. Failure to detect diseases in a timely and accurate manner will lead to misjudgment and delay of the disease, affecting the public and customers' timely medical treatment, damaging health and even endangering life. Summary of the invention

[0004] In view of the above problems in the prior art, the present invention provides an all-in-one machine for selling and automatically detecting nucleic acid molecules.

[0005] In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention is as follows:

[0006] Provided is an all-in-one machine for selling and automatically detecting nucleic acid molecules, including a cabinet body, in which a product selling mechanism is arranged, and an operation panel and a nucleic acid detecting mechanism are arranged on one side of the product selling mechanism. The product selling mechanism includes a cabinet for arranging and storing medicines and medical supplies such as test kits, cooling drugs, masks, etc.

[0007] The product selling mechanism includes a cabinet for storing test kits, wherein a plurality of rows of shelves are arranged in parallel in the cabinet, and a plurality of cargo slots are arranged in parallel on each row of shelves. A delivery channel is arranged on one side of the shelf in the cabinet, and the cargo slot is open at one end close to the delivery channel. A pickup warehouse connected to the bottom of the delivery channel is arranged on the cabinet below the cabinet, and a pickup component for taking the test kits in the cargo slots and delivering them to the pickup warehouse is arranged in the cabinet;

[0008] The nucleic acid detection mechanism includes a detection box, a detection window connected to the detection box is opened on the cabinet, an isothermal fluorescence detector for detecting single sample tubes or sample linked tubes is arranged in the detection box, a trash can is arranged below the detection box in the cabinet, and a cleaning component for cleaning the sample linked tubes on the isothermal fluorescence detector is arranged in the detection box.

[0009] Furthermore, the picking component includes a first picking bracket installed in the cabinet in a vertically lifting manner, a second picking bracket is arranged on the first picking bracket for horizontal movement along the arrangement direction of the cargo slots, a transfer slot is arranged on the second picking bracket, and a pushing component for taking out the test kit from the cargo slot is arranged on the second picking bracket;

[0010] The cargo pushing assembly includes a spiral rod rotatably arranged in the cargo trough, a storage cavity for placing the test kit is formed between adjacent spirals on the spiral rod, the cargo trough is extended at one end of the spiral rod close to the cargo delivery channel and a driving disk is fixedly arranged, a third cargo pickup bracket is arranged on the second cargo pickup bracket, a first driving member for driving the third cargo pickup bracket to move axially parallel to the spiral rod is arranged on the second cargo pickup bracket, a socket for axially cooperating with the driving disk is arranged on the third cargo pickup bracket, a second driving member for driving the socket to rotate is arranged on the third cargo pickup bracket, a transfer slot is fixedly arranged on the third cargo pickup bracket and is located below the socket, and a first opening and closing assembly is arranged at the bottom of the transfer slot.

[0011] Furthermore, the first opening and closing component includes a bottom plate, the bottom of the transfer slot is open, the bottom plate is hingedly arranged at the bottom of the transfer slot, the transfer slot is provided with a third driving member for driving the bottom plate to rotate, and a cargo inlet is opened at the top of the cargo bin, which moves to the top of the cargo inlet through the transfer slot and opens the bottom plate to deliver the test kit into the cargo inlet.

[0012] Further, the cleaning assembly includes a pneumatic clamp, a first cleaning bracket is driven and slidably arranged on the inner top wall of the detection box, a second cleaning bracket is vertically driven and lifted on the first cleaning bracket, the pneumatic clamp is arranged on the second cleaning bracket, a waste discharge channel is vertically arranged on one side of the detection box, a waste discharge window for the first cleaning bracket to enter / exit the waste discharge channel is opened on the side wall of the detection box, the bottom end of the waste discharge channel is open and faces the trash can, and the top of the trash can is open;

[0013] An isolation door for opening and closing the waste discharge window is rotatably arranged in the waste discharge window, and a second opening and closing component for driving the isolation door to rotate is arranged in the detection box.

[0014] Furthermore, the second opening and closing assembly includes a torsion spring, an electromagnet, a sealing strip and a push rod. The torsion spring is arranged on the rotating shaft of the isolation door. The torsion spring is used to drive the isolation door to rotate to close the waste discharge window. The push rod is symmetrically fixed on the outside of the first cleaning bracket. The waste discharge window is located on the side wall of the detection box away from the shelf. A roller for rolling contact with the isolation door is arranged on the end of the push rod away from the shelf. The sealing strip is arranged on the edge of the isolation door for contacting the detection box and the edges of the two isolation doors close to each other. The electromagnet is used to magnetically adsorb the isolation door to seal and fit the detection box.

[0015] Furthermore, a refrigeration system is arranged in the cabinet, and the refrigeration system comprises a compressor, a condenser, an expansion valve and an evaporator which are connected in a sequential cycle, and the evaporator is arranged in the cabinet;

[0016] A preheating system is arranged in the detection box, and the preheating system includes an air inlet fan, a first air inlet pipe and a first electric heating wire. The air inlet of the air inlet fan is connected to a second air inlet pipe connected to the outside of the cabinet. One end of the first air inlet pipe is connected to the air outlet of the air inlet fan, and the other end is connected to the inside of the detection box. A plurality of air outlet holes are opened in the detection box, and the air outlet is connected to an air outlet pipe connected to the outside of the cabinet. The first electric heating wire is arranged at one end of the first air inlet pipe close to the detection box.

[0017] A temperature insulation board is arranged between the cabinet and the detection box.

[0018] Furthermore, the first air inlet pipe passes through the condenser, and part of the heat emitted by the condenser is taken away by the first air inlet pipe. Electronic valves are provided on the second air inlet pipe and the air outlet pipe. A circulation pipe is connected to the side of the second air inlet pipe located near the detection box at the electronic valve. The end of the circulation pipe away from the air outlet pipe is connected to the side of the electronic valve on the first air inlet pipe near the air inlet fan.

[0019] Furthermore, an end of the second air inlet pipe away from the air inlet fan is connected to an air filter.

[0020] Furthermore, a disinfection component is arranged in the detection box, and the disinfection component includes a negative ion generator and a plurality of nozzles. The negative ion generator is arranged in the first air inlet pipe, and the nozzle is arranged on the inner wall of the detection box. The nozzle is connected to a pipe, and the pipe is connected to an external disinfection water supply system.

[0021] Furthermore, the isothermal fluorescence detector is provided with a tube rack for placing sample tubes, the tube rack includes a fixed frame and a movable frame, a second heating wire is arranged in the fixed frame, the movable frame is vertically slidably arranged directly above the fixed frame, and the movable frame and the fixed frame are coaxially provided with sockets for inserting the sample tubes, and a fourth driving member for driving the movable frame to vertically rise and fall is arranged in the isothermal fluorescence detector.

[0022] The beneficial effects of the present invention are as follows: 1. In addition to nucleic acid detection kits, masks, antipyretics, cold medicines and other medicines and sanitary products can also be placed in the product sales mechanism. Customers can operate the all-in-one machine at any time according to their needs, and choose to purchase nucleic acid detection kits or medicines from the operation panel. The second picking bracket in the cabinet moves to the exit end of the cargo slot where the goods to be shipped are located, and the goods are pushed out of the cargo slot and fall into the transfer slot by the pushing component. The second picking bracket then moves to the bottom of the shipping channel, and the first opening and closing component at the bottom of the transfer slot opens, and the goods fall into the picking bin below for customers to take by themselves, which improves the convenience of purchasing medical and sanitary products such as nucleic acid detection kits;

[0023] 2. When testing the nucleic acid sample tube, select the type of nucleic acid to be tested from the operation panel, put the sample tube into the isothermal fluorescence detector in the test box, and after the isothermal fluorescence detector performs the test by itself, the control system will feed back the test results on the operation panel for the customer to view, or send the results to the customer's communication terminal via network transmission, so as to improve the accuracy of the test result judgment;

[0024] 3. The integrated machine for selling and automatically detecting nucleic acid molecules occupies a small area and has low cost. It can be deployed in large numbers in urban communities, residential areas and streets. It has the functions of automatic selling of medicines and sanitary products and automatic detection of sample tubes for nucleic acid testing. It provides great convenience for urban residents to purchase medicines in a timely manner throughout the day and accurately and automatically detect nucleic acid results, greatly reducing the burden on the medical system of urban hospitals. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the overall structure of the integrated machine for selling and automatically detecting nucleic acid molecules according to the embodiment of the present application;

[0026] Figure 2 This is a schematic diagram of the internal structure of the integrated machine for selling and automatically detecting nucleic acid molecules according to the embodiment of the present application;

[0027] Figure 3 This is a schematic diagram of the internal structure of a cabinet according to an embodiment of the present application;

[0028] Figure 4 for Figure 3 A magnified schematic diagram of part A;

[0029] Figure 5 A partial cross-sectional schematic diagram of a nucleic acid detection mechanism according to an embodiment of the present application;

[0030] Figure 6 for Figure 5 A magnified schematic diagram of part B;

[0031] Figure 7 This is a schematic diagram of the structure of the refrigeration system and preheating system in the cabinet according to an embodiment of the present application.

[0032] Among them, 1, cabinet; 11, picking warehouse; 13, operation panel; 2, cabinet; 21, shelf; 22, cargo slot; 23, shipping channel; 3, detection box; 31, isothermal fluorescence detector; 32, trash can; 41, first picking bracket; 42, second picking bracket; 43, transfer slot; 431, bottom plate; 432, third driving member; 44, spiral rod; 45, driving disk; 451, connecting slot; 46, third picking bracket; 461, first driving member; 47, socket; 471, second driving member; 51, pneumatic gripper; 5 2. First cleaning bracket; 53. Second cleaning bracket; 54. Waste discharge channel; 55. Waste discharge window; 56. Isolation door; 57. Sealing strip; 58. Push rod; 581. Roller; 61. Compressor; 62. Condenser; 63. Expansion valve; 64. Evaporator; 65. Insulation board; 71. Air inlet fan; 72. First air inlet pipe; 73. First electric heating wire; 74. Second air inlet pipe; 75. Air outlet pipe; 76. Circulation pipe; 77. Air filter; 78. Negative ion generator; 79. Nozzle; 81. Fixed bracket; 82. Movable bracket. DETAILED DESCRIPTION

[0033] In order to better understand the above technical solution, the above technical solution will be described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0034] The embodiment of the present invention provides a machine for selling and automatically detecting nucleic acid molecules, referring to Figure 1 and Figure 2 , including a cabinet 1, the interior of the cabinet 1 is hollow, and a product selling mechanism is provided on one side of the cabinet 1. The product selling mechanism includes a cabinet for arranging and storing products, and the products include medicines and medical supplies such as test kits, antipyretics, masks, etc. An operation panel 13 and a nucleic acid detection mechanism are provided on the other side of the product selling mechanism. The product selling mechanism is used to automatically sell common medicines and medical supplies such as test kits, masks, antipyretics, cold medicines, gauze, etc. The nucleic acid detection mechanism is used to automatically detect the sample single tube or sample joint tube of the nucleic acid reagent put in by the customer. The following description is based on the sample joint tube. A control system is provided in the cabinet 1, and the control system is electrically connected to each actuator in the cabinet 1 and the operation panel 13. The operation panel 13 can be a touch screen or a combination of buttons and a display screen. Through the operation panel 13, operations such as operation selection, viewing related information of the test kit, operation payment, operation of nucleic acid detection, and viewing of test reports can be performed.

[0035] Reference Figure 3 and Figure 4The product selling mechanism includes a cabinet 2 for storing test kits. The side of the cabinet 2 on the same side as the operation panel 13 can be designed as a double-layer glass or plastic transparent window, so that customers can observe the distribution of different test kits in the cabinet 2 and the inventory of different types of test kits through the window. Multiple rows of shelves 21 are arranged in parallel in the cabinet 2, and multiple cargo slots 22 are arranged in parallel on each row of the shelves 21. Multiple boxes of test kits are arranged in each cargo slot 22. A shipping channel 23 is provided on one side of the shelf 21 in the cabinet 1, and each cargo slot 22 is opened at the end near the shipping channel 23. A pickup warehouse 11 is provided below the cabinet 2 in the cabinet 1. The top of the pickup warehouse 11 is open, and the pickup warehouse 11 is connected to the bottom of the shipping channel 23. A pickup port is provided on the outer wall of the cabinet 1 below the cabinet 2. Customers can reach into the pickup warehouse 11 at the pickup port outside the cabinet 1 to take out the test kits that fall into the pickup warehouse 11. The flap plate can be rotated freely at the pickup port, and the pickup port can be closed by rotating the flap plate freely to prevent debris from entering the pickup bin 11. Customers can select the goods and quantity to be purchased from the operation panel 13, and the product sales mechanism will automatically deliver the corresponding quantity of goods to the pickup bin 11, which is convenient for customers to shop.

[0036] The cabinet is provided with a pickup assembly for picking up the test kits in the cargo slot 22 and delivering them to the pickup bin 11. In the embodiment of the present application, the pickup assembly includes a first pickup bracket 41, a second pickup bracket 42, a transfer slot 43 and a pushing assembly. A first track is vertically installed in the cabinet 1, and the first pickup bracket 41 is slidably installed on the first track. A first linear slide is provided on the first track, and the first linear slide is used to drive the first pickup bracket 41 to slide vertically. A second track is installed on the first pickup bracket 41 parallel to the arrangement direction of the cargo slot 22, and the second pickup bracket 42 is slidably installed on the second track. A second linear slide is provided on the second pickup bracket 42, and the second linear slide is used to drive the second pickup bracket 42 to slide horizontally. The transfer slot 43 is arranged on the second picking bracket 42. When the first picking bracket 41 and the second picking bracket 42 are slid, the transfer slot 43 is moved to the lower part of the opening of the cargo slot 22 where the reagent kit needs to be taken out, and the reagent kit in the cargo slot 22 is pushed to the exit of the cargo slot 22 by the pushing component, and the reagent kit is pushed into the transfer slot 43 by pushing a reagent kit thickness each time. The transfer slot 43 moves down to the bottom of the delivery channel 23 near the picking bin 11. The bottom of the transfer slot 43 is open and is designed with a first opening and closing component. When the first opening and closing component is actuated, the reagent kit in the transfer slot 43 can fall into the picking bin 11, which reduces the falling height of the reagent kit, protects the reagent kit, reduces the reagent bubble phenomenon, and avoids the reagent kit from being stuck in the delivery channel 23.

[0037] In the embodiment of the present application, the push assembly includes a screw rod 44 rotatably mounted in the cargo slot 22, the screw rod 44 is spirally arranged, and a storage cavity for placing the test kit is formed between adjacent spirals on the screw rod 44. The end of the screw rod 44 close to the delivery channel 23 extends out of the cargo slot 22, and a driving disk 45 is fixedly installed, the driving disk 45 is coaxial with the screw rod 44, and a plurality of connecting grooves 451 are opened on the driving disk 45. A third cargo pickup bracket 46 is provided on the side of the second cargo pickup house close to the shelf 21, the second cargo pickup bracket 42 is provided with a first driving member 461 for driving the third cargo pickup bracket 46 to move parallel to the axial direction of the screw rod, and the third cargo pickup bracket 46 is provided with a plug-in seat 47, and a connecting block adapted to the connecting groove 451 on the driving disk 45 is fixed on the plug-in seat 47. When the third cargo pickup bracket 46 moves toward the shelf 21, the connecting block on the plug-in seat 47 can be plugged and matched with the connecting groove 451 on the driving disk 45 on the shelf 21 that is directly opposite to it. The third picking bracket 46 is provided with a second driving member 471 for driving the plug-in socket 47 to rotate. The second driving member 471 can drive the spiral rod 44 in the corresponding cargo slot 22 to rotate, thereby pushing the reagent kit in the cargo slot 22 to move. After the reagent kit is pushed out of the cargo slot 22 with the spiral rod 44, the reagent kit loses the support of the cargo slot 22 and falls into the transfer slot 43 below. In the embodiment of the present application, the transfer slot 43 is fixedly mounted on the third picking bracket 46 and is located below the plug-in socket 47. The transfer slot 43 moves with the third picking bracket 46 to approach the shelf 21, so that the transfer slot 43 can extend into the bottom of the cargo slot 22 to accurately receive the reagent kit, and when the transfer slot 43 moves with the second picking bracket 42, the transfer slot 43 moves away from the shelf 21 and does not interfere with the shelf 21.

[0038] Through the push assembly in the embodiment of the present application, the plug-in socket 47 can be driven to rotate only by a second driving member 471, so as to specifically control the rotation of the spiral rod 44 in any cargo slot 22. Compared with the traditional vending machine in which each cargo slot 22 is designed with an electric pusher, the installation space and manufacturing cost are saved. Moreover, by placing the reagent kit between every two spirals of the spiral rod 44, the spirals of the spiral rod 44 are limited on both sides of the reagent kit, so that the reagent kit can be prevented from tipping over. The pushing force is strong, and the reagent kits are arranged neatly. When the shelf 21 is arranged horizontally, the reagent kits near the exit of the cargo slot 22 can also be stably placed to prevent the reagent kits from tipping over at the exit of the cargo slot 22.

[0039] In the embodiment of the present application, the first driving member 461 can be an electric push rod 58, which is fixed on the second picking bracket 42, and the output end of the electric push rod 58 is fixedly connected to the third picking bracket 46. The second driving member 471 can be a servo motor, and the output shaft of the servo motor is coaxially fixedly connected to the plug socket 47. In order to make the plug socket 47 and the drive disk 45 match smoothly, further, a chamfered surface can be designed for the end of the connecting block, and a guide surface can be designed at the opening edge of the connecting groove 451, so that when the plug socket 47 moves closer to the drive disk 45, the two can be plugged smoothly.

[0040] The first opening and closing component includes a bottom plate 431, the bottom of the transfer slot 43 is open, the bottom plate 431 is hingedly arranged at the bottom of the transfer slot 43, and the transfer slot 43 is provided with a third driving member 432 for driving the bottom plate 431 to rotate. A cargo inlet is opened at the top of the cargo pickup bin 11, and the transfer slot 43 is moved to the top of the cargo inlet and the bottom plate 431 is opened to deliver the test kit into the cargo inlet.

[0041] Reference Figure 5 and Figure 6 , the nucleic acid detection mechanism on the cabinet 1 includes a detection box 3, a detection window connected to the detection box 3 is opened on the cabinet 1, an isothermal fluorescence detector 31 for detecting sample tubes is arranged in the detection box 3, a trash can 32 is arranged below the detection box 3 in the cabinet 1, and a cleaning component for cleaning the sample tubes on the isothermal fluorescence detector 31 is arranged in the detection box 3. A detection cabinet door can be set on the cabinet 1, and the detection cabinet door is used to close the detection window. The detection cabinet door is opened when the nucleic acid test is performed by operating the operation panel 13, and the structure such as the sensor determines whether the detection cabinet door is closed. After the customer puts the sample tube into the isothermal fluorescence detector 31, the cabinet door is closed, and the detection is controlled to start from the operation panel 13. After the detection is completed, the sample tube on the isothermal fluorescence detector 31 is automatically cleaned by the cleaning component. The outer wall of the cabinet 1 can be wiped and cleaned regularly by the equipment maintenance personnel, and can also be cleaned by the replenishment personnel while replenishing the product sales mechanism.

[0042] Specifically, the cleaning assembly includes a pneumatic clamp 51, a third track and a third linear slide rail matched therewith are installed on the inner top wall of the detection box 3, a first cleaning bracket 52 is driven and slidably installed on the third track, a fourth track and a fourth linear slide rail matched therewith are vertically provided on the first bracket, a second cleaning bracket 53 is driven and slidably installed on the first track, and the pneumatic clamp 51 is fixedly installed on the second cleaning bracket 53. A vertical waste discharge channel 54 is provided on the side of the detection box 3 away from the cabinet 2, and a waste discharge window 55 is provided on the side wall of the detection box 3 away from the cabinet 2 to connect the detection box 3 with the waste discharge channel 54. The top of the trash bin 32 is open, and the bottom of the waste discharge channel 54 is open and faces the trash bin 32. Through the action of the first cleaning bracket 52, the second cleaning bracket 53 and the pneumatic clamp 51, the sample tube after detection can be clamped from the isothermal fluorescence detector 31. Then, by opening the waste discharge window 55, the first cleaning bracket 52 and the second cleaning bracket 53 and the pneumatic clamp 51 thereon can be moved in / out of the waste discharge window 55, so that the sample tube is moved into the waste discharge channel 54 to be loosened, and the sample tube is thrown into the trash bin 32. A waste discharge cabinet door can be set on the outer wall of the cabinet 1, and the waste discharge cabinet door is locked by a cam lock. After the staff opens the waste discharge cabinet door, the trash bin 32 in the cabinet 1 can be taken out for cleaning.

[0043] In the embodiment of the present application, an isolation door 56 for opening and closing the waste discharge window 55 is rotatably provided in the waste discharge window 55, and a second opening and closing assembly for driving the isolation door 56 to rotate is provided in the detection box 3. The second opening and closing assembly may specifically include a torsion spring (not shown in the figure), an electromagnet (not shown in the figure), a sealing strip 57 and a push rod 58. The isolation door 56 is rotatably connected to the outer wall of the detection box 3 through a rotating shaft. The torsion spring is provided on the rotating shaft of the isolation door 56. Under the action of the elastic force of the torsion spring, the isolation door 56 is driven to rotate to close the waste discharge window 55. The push rod 58 is symmetrically fixed on both sides of the first cleaning bracket 52. During the movement of the first cleaning bracket 52, the push rod 58 contacts the isolation door 56 and pushes the isolation door 56 to overcome the elastic force of the torsion spring and open. When the first cleaning bracket 52 moves back into the detection box, the isolation door 56 rotates under the action of the torsion spring to close the waste discharge window 55.

[0044] Furthermore, sealing strips 57 are provided on the edges of the isolation door 56 that are used to contact the detection box 3 and on the edges where the two isolation doors 56 are close to each other. An electromagnet is installed on the detection box 3 to adsorb the isolation door 56 in the state of closing the waste discharge window 55, so that the isolation door 56 can maintain the state of pressure-sealing the waste discharge window 55, thereby completely sealing the waste discharge window 55 through the two isolation doors 56.

[0045] Furthermore, a roller 581 is rotatably installed at one end of the push rod 58 away from the cabinet 2. When the push rod 58 pushes open the isolation door 56, the roller 581 on the push rod 58 rolls in contact with the isolation door 56 to reduce friction and enable the isolation door 56 to open smoothly.

[0046] Reference Figure 7 In order to better preserve the test kit in the cabinet 2, a refrigeration system is provided in the cabinet 1. The refrigeration system includes a compressor 61, a condenser 62, an expansion valve 63 and an evaporator 64 which are connected in a sequential cycle. The evaporator 64 is located in the cabinet 2. Through the operation of the refrigeration system, the temperature in the cabinet 2 can be reduced to create a low-temperature environment, which can better preserve the nucleic acid detection reagents.

[0047] During the operation of the detection box 3, in order to make the environment in the detection box 3 suitable, a preheating system is provided in the detection box 3, and the preheating system includes an air inlet fan 71, a first air inlet pipe 72 and a first electric heating wire 73. The air inlet of the air inlet fan 71 is connected to the second air inlet pipe 74, and the second air inlet pipe 74 is connected to the outside. One end of the first air inlet pipe 72 is connected to the air outlet of the air inlet fan 71, and the other end is connected to the inside of the detection box 3. A plurality of air outlet holes are provided in the detection box 3, and the air outlet is connected to the air outlet pipe 75, and the other end of the air outlet pipe 75 is connected to the outside of the cabinet 1. The first electric heating wire 73 is provided at one end of the first air inlet pipe 72 close to the detection box 3. When preheating the environment in the detection box 3, the first electric heating wire 73 and the air inlet fan 71 are started to continuously supply air to the detection box 3. After being heated by the first electric heating wire 73, the air supply temperature is increased, thereby heating the environment in the detection box 3. In order to prevent the temperature of the detection box 3 from affecting the preservation of the samples in the cabinet 2 during the preheating process, a temperature insulation board 65 is provided in the cabinet 1 between the cabinet 2 and the detection box 3 .

[0048] Furthermore, in order to reduce the energy loss of the all-in-one machine, in the embodiment of the present application, the first air inlet duct 72 passes through the condenser 62, and the first air inlet duct 72 is tightly fitted with part of the heat sink in the condenser 62. The first air inlet duct 72 adopts a metal copper tube, and utilizes the heat dissipated when the condenser 62 is working to heat the low-temperature air entering the first air inlet duct 72, thereby improving the preheating efficiency of the detection box 3, and when the preheating system stops running, the condenser 62 can still work normally.

[0049] Furthermore, the second air inlet pipe 74 and the air outlet pipe 75 are both provided with electronic valves, and the second air inlet pipe 74 is connected to a circulation pipe 76 on the side of the electronic valve close to the detection box 3, and the circulation pipe is provided with an electronic valve, and the end of the circulation pipe 76 away from the air outlet pipe 75 is connected to the side of the electronic valve on the first air inlet pipe 72 close to the air inlet fan 71. After the electronic valves on the second air inlet pipe 74 and the air outlet pipe 75 are closed, the second air inlet pipe 74, the first air inlet pipe 72, the detection box 3 and the air outlet pipe 75 in the preheating system form a circulation loop, so that the hot air circulates and heats continuously, which greatly improves the preheating efficiency of the detection box 3.

[0050] In order to prevent impurities from being contained in the hot air introduced into the detection box 3 during the preheating process, in the embodiment of the present application, an air filter 77 is connected to the end of the second air inlet duct 74 away from the air inlet fan 71, which can filter impurities in the air entering the detection box 3 from the second air inlet duct 74.

[0051] Before and after the nucleic acid molecule detection in the detection box 3, it is also necessary to disinfect the environment inside the detection box 3 to eliminate the influence of miscellaneous bacteria on the detection results. A disinfection component is provided in the detection box 3, and the disinfection component includes a negative ion generator 78 and a plurality of nozzles 79. The negative ion generator 78 is provided in the first air inlet pipe 72. When the detection box 3 is preheated, the negative ion generator 78 is started, and the detection box 3 is preheated while the detection box 3 is preliminarily disinfected and sterilized, which is suitable for rapid disinfection before the nucleic acid molecule detection operation. The nozzle 79 is provided on the inner wall of the detection box 3, and a pipe is connected to the nozzle 79. The pipe is externally connected to a disinfection water supply system. The disinfection water supply system includes at least a water tank containing disinfection water and a water pump for pumping disinfection water. By spraying disinfection water on the inner wall of the detection box 3, the interior of the detection box 3 can be fully disinfected, which is suitable for deep disinfection after the nucleic acid molecule detection operation.

[0052] In the embodiment of the present application, a tube rack for placing sample tubes is provided on the isothermal fluorescence detector 31, and the tube rack includes a fixed frame 81 and a movable frame 82. The movable frame 82 is vertically slidably arranged just above the fixed frame 81. Both the movable frame 82 and the fixed frame 81 are coaxially provided with jacks for inserting the sample tubes. A fourth driving member for driving the movable frame 82 to vertically rise and fall is provided in the isothermal fluorescence detector 31, and the fourth driving member can be a cylinder or an electric push rod 58. When the customer performs nucleic acid testing, the sample tube is plugged into the movable frame 82, and the fourth driving member is actuated to lower the movable frame 82, so that the sample tube is inserted into the fixed frame 81 for testing the sample tube. After the test is completed, the fourth driving member drives the movable frame 82 to rise, so that the pneumatic clamp 51 can accurately clamp the sample tube. Furthermore, a second heating wire is installed in the fixed frame 81. After the detection cabinet door is closed, the second heating wire starts to heat the sample to improve the uniformity of the temperature in the sample and avoid the influence of the edge effect on the detection. The heating temperature and heating time of the second heating wire are controlled by the control system. After the heating is completed, the light source in the isothermal fluorescence detector 31 emits laser to irradiate the sample tube, and collects the fluorescence in the sample tube for analysis and reading to obtain the detection result. The control system stores the detection result for the customer to query and print on the operation panel 13 or sends the detection result to the customer's communication terminal in the form of network communication. After the customer starts the nucleic acid detection operation from the operation panel 13, the sample tube is placed in the detection box 3. The detection process of the sample in the sample tube and the subsequent sample tube cleaning process are automatically performed by the all-in-one machine. The customer only needs to obtain the detection result from the operation panel 13, which greatly reduces the customer's operation process and improves the customer's use experience.

[0053] It should be understood by those skilled in the art that, although preferred embodiments of the present invention have been described, additional changes and modifications may be made to these embodiments once those skilled in the art are aware of the basic inventive concepts. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention. Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A machine for selling and automatically detecting nucleic acid molecules, characterized in that: It comprises a cabinet (1), wherein a product selling mechanism is provided in the cabinet (1), and an operation panel (13) and a nucleic acid detection mechanism are provided on one side of the product selling mechanism; The product selling mechanism comprises a cabinet (2) for storing test kits, wherein a plurality of rows of shelves (21) are arranged in parallel in the cabinet (2), and a plurality of cargo slots (22) are arranged in parallel on each row of the shelves (21); a delivery channel (23) is arranged on one side of the shelves (21) in the cabinet (1), and one end of the cargo slot (22) close to the delivery channel (23) is open; a cargo pickup warehouse (11) connected to the bottom of the delivery channel (23) is arranged on the cabinet (1) below the cabinet (2); and a cargo pickup component for taking the test kits in the cargo slot (22) and delivering them to the cargo pickup warehouse (11) is arranged in the cabinet (2); The nucleic acid detection mechanism comprises a detection box (3); a detection window connected to the detection box (3) is provided on the cabinet (1); an isothermal fluorescence detector (31) for detecting a single sample tube or a linked sample tube is arranged in the detection box (3); a trash bin (32) is arranged below the detection box (3) in the cabinet (1); and a cleaning component for cleaning the linked sample tubes on the isothermal fluorescence detector (31) is arranged in the detection box (3).

2. The integrated machine for selling and automatically detecting nucleic acid molecules according to claim 1, characterized in that: The picking component comprises a first picking bracket (41) vertically lifted and installed in the cabinet (1); a second picking bracket (42) is horizontally movable on the first picking bracket (41) along the arrangement direction of the cargo slot (22); a transfer slot (43) is arranged on the second picking bracket (42); and a pushing component for taking out the reagent kit from the cargo slot (22) is arranged on the second picking bracket (42); The pushing assembly comprises a spiral rod (44) rotatably arranged in the cargo groove (22), a storage cavity for placing the test kit is formed between adjacent spirals on the spiral rod (44), one end of the spiral rod (44) close to the delivery channel (23) extends out of the cargo groove (22) and is fixedly provided with a driving disk (45), a third cargo picking bracket (46) is arranged on the second cargo picking bracket (42), and a device for driving the third cargo picking bracket (46) to move parallel to the spiral rod (44) is arranged on the second cargo picking bracket (42). A first driving member (461) for axially moving the rotating rod (44), a plug-in socket (47) for axially cooperating with the driving disk (45) is provided on the third picking-up bracket (46), a second driving member (471) for driving the plug-in socket (47) to rotate is provided on the third picking-up bracket (46), the transfer slot (43) is fixedly arranged on the third picking-up bracket (46) and is located below the plug-in socket (47), and a first opening and closing component is provided at the bottom of the transfer slot (43).

3. The integrated machine for selling and automatically detecting nucleic acid molecules according to claim 2, characterized in that: The first opening and closing component includes a bottom plate (431), the bottom of the transfer slot (43) is open, the bottom plate (431) is hingedly arranged at the bottom of the transfer slot (43), and the transfer slot (43) is provided with a third driving member (432) for driving the bottom plate (431) to rotate. The top of the picking warehouse (11) is provided with a cargo inlet, and the transfer slot (43) is moved to the top of the cargo inlet and the bottom plate (431) is opened to deliver the test kit into the cargo inlet.

4. The integrated machine for selling and automatically detecting nucleic acid molecules according to claim 1, characterized in that: The cleaning assembly comprises a pneumatic clamp (51), a first cleaning bracket (52) is driven and slidably arranged on the inner top wall of the detection box (3), a second cleaning bracket (53) is vertically driven and lifted on the first cleaning bracket (52), the pneumatic clamp (51) is arranged on the second cleaning bracket (53), a waste discharge channel (54) is vertically arranged on one side of the detection box (3), a waste discharge window (55) is provided on the side wall of the detection box (3) for the first cleaning bracket (52) to enter / exit the waste discharge channel (54), the bottom end of the waste discharge channel (54) is open and faces the garbage bin (32), and the top of the garbage bin (32) is open; An isolation door (56) for opening and closing the waste discharge window (55) is rotatably provided in the waste discharge window (55), and a second opening and closing component for driving the isolation door (56) to rotate is provided in the detection box (3).

5. The integrated machine for selling and automatically detecting nucleic acid molecules according to claim 4, characterized in that: The second opening and closing assembly comprises a torsion spring, an electromagnet, a sealing strip (57) and a push rod (58); the torsion spring is arranged on the rotating shaft of the isolation door (56); the torsion spring is used to drive the isolation door (56) to rotate until the waste discharge window (55) is closed; the push rod (58) is symmetrically fixedly arranged on the outer side of the first cleaning bracket (52); the waste discharge window (55) is located on the side wall of the detection box (3) away from the shelf (21); a roller (581) for rolling contact with the isolation door (56) is arranged at one end of the push rod (58) away from the shelf (21); the sealing strip (57) is arranged on the isolation door (56) for contacting the edge of the detection box (3) and the edges of the two isolation doors (56) close to each other; the electromagnet is used to magnetically adsorb the isolation door (56) to seal and fit the detection box (3).

6. The integrated machine for selling and automatically detecting nucleic acid molecules according to claim 1, characterized in that: A refrigeration system is arranged in the cabinet (2), the refrigeration system comprising a compressor (61), a condenser (62), an expansion valve (63) and an evaporator (64) which are connected in a sequential cycle, and the evaporator (64) is arranged in the cabinet (2); The detection box (3) is provided with a preheating system, the preheating system comprising an air inlet fan (71), a first air inlet pipe (72) and a first electric heating wire (73); the air inlet of the air inlet fan (71) is connected to a second air inlet pipe (74) connected to the outside of the cabinet (1); one end of the first air inlet pipe (72) is connected to the air outlet of the air inlet fan (71) and the other end is connected to the inside of the detection box (3); a plurality of air outlet holes are provided in the detection box (3); the air outlet is connected to an air outlet pipe (75) connected to the outside of the cabinet (1); the first electric heating wire (73) is arranged at one end of the first air inlet pipe (72) close to the detection box (3); A temperature insulation board (65) is provided between the cabinet (2) and the detection box (3).

7. The integrated machine for selling and automatically detecting nucleic acid molecules according to claim 6, characterized in that: The first air inlet pipe (72) passes through the condenser (62), and takes away part of the heat emitted by the condenser (62) through the first air inlet pipe (72); the second air inlet pipe (74) and the air outlet pipe (75) are both provided with electronic valves; a circulation pipe (76) is connected to the side of the second air inlet pipe (74) located near the electronic valve of the detection box (3); and an end of the circulation pipe (76) away from the air outlet pipe (75) is connected to the side of the electronic valve of the first air inlet pipe (72) near the air inlet fan (71).

8. The integrated machine for selling and automatically detecting nucleic acid molecules according to claim 7, characterized in that: An end of the second air inlet pipe (74) away from the air inlet fan (71) is connected to an air filter (77).

9. The integrated machine for selling and automatically detecting nucleic acid molecules according to claim 8, characterized in that: A disinfection component is arranged in the detection box (3), and the disinfection component includes a negative ion generator (78) and a plurality of nozzles (79). The negative ion generator (78) is arranged in the first air inlet pipe (72), and the nozzle (79) is arranged on the inner wall of the detection box (3). The nozzle (79) is connected to a pipeline, and the pipeline is externally connected to a disinfection water supply system.

10. The integrated machine for selling and automatically detecting nucleic acid molecules according to any one of claims 1 to 9, characterized in that: The isothermal fluorescence detector (31) is provided with a tube rack for placing sample tubes, the tube rack comprises a fixed frame (81) and a movable frame (82), a second heating wire is arranged in the fixed frame (81), the movable frame (82) is vertically slidably arranged just above the fixed frame (81), the movable frame (82) and the fixed frame (81) are coaxially provided with jacks for inserting the sample tubes, and a fourth driving member for driving the movable frame (82) to vertically rise and fall is arranged in the isothermal fluorescence detector (31).

Images