Grafting seedling raising method capable of improving disease resistance of tomatoes

Abstract

The invention provides a grafting seedling raising method capable of improving disease resistance of tomatoes. The grafting seedling raising method comprises the following steps of tree tomato and tomato seedling raising, seedling stage management, grafting and grafting later-period management. According to the method, woody tomatoes are selected and have the advantages of being high in growth speed and good in disease resistance, and the disease resistance and yield of plants can be greatly improved when the woody tomatoes serve as rootstocks; in the growth process of tomatoes, the morbidity is high, especially bacterial wilt and root-knot nematode are serious, and when the morbidity is serious, no harvest is caused, and the fundamental problem can be solved through grafting. The tree tomato seeds are large in quantity, easy to breed and high in budding rate; by using the special scion soaking solution, the wound healing speed of grafting is greatly increased, and the healing effect is good. The grafting seedling raising method is simple and easy to implement, convenient to manage, low in cost, high in yield and suitable for large-scale popularization, the quality and yield of tomatoes can be greatly improved, and remarkable economic benefits and ecological benefits are achieved.

Description

Technical Field

[0001] The invention relates to the technical field of crop cultivation, and in particular to a grafting seedling raising method capable of improving disease resistance of tomatoes. Background Art

[0002] Tree tomato (Cyphomandra betacea Sendt.) is a small tree or sometimes shrub of the genus Cyphomandra in the Solanaceae family. It is also known as the sour tomato and woody tomato. It is native to South America and has been introduced to tropical and subtropical regions of the world. It is cultivated in Yunnan and southern Tibet in my country. Tree tomato has a well-developed root system and is not demanding on soil, making it a good rootstock variety.

[0003] Tomato is a plant of the Solanaceae family, an annual or limited perennial herb. The fruit is rich in vitamins, carotene, protein, sugar, organic acid, calcium, minerals and other substances. The content of vitamin C and carotene is extremely high, which is beneficial to control heart disease and coronary artery sclerosis, lower cholesterol, and has multiple effects such as anti-oxidation and anti-aging. Tomato is one of the vegetable crops with the largest cultivation area and consumption in my country.

[0004] In recent years, with the continuous increase in market demand and the rapid development of facility cultivation, the area of ​​tomato facility cultivation and open-field cultivation has continued to expand, and the phenomenon of repeated tomato planting has become more and more common. Unbalanced fertilizer application, soil secondary salinization and soil acidification caused by the closed environment of facility cultivation, continuous cropping obstacles, bacterial wilt, root-knot nematodes, blight and other diseases have become more and more serious, which seriously inhibits the growth and development of tomato crops and reduces tomato yield and quality.

[0005] Grafting is a cultivation technique commonly used in horticultural crops to cope with adverse environments. Through the grafting method, excellent rootstock varieties that can effectively improve crop resistance are selected, which has a significant role in promoting the development of my country's agriculture. The operation is simple and can significantly improve the resistance of tomatoes to adverse environments. There is huge room for application in my country's tomato industry. Choosing suitable rootstock grafting can not only improve the plant's ability to resist biotic and abiotic stresses; it can also greatly improve the yield and quality of tomato fruits.

[0006] However, the existing tomato grafting methods have low grafting efficiency and survival rate, poor quality of selected rootstocks, and low effect on the growth and disease resistance of grafted plants. They cannot meet the needs of sustainable development of large-scale tomato cultivation technology and need further improvement. Summary of the invention

[0007] In view of this, the purpose of the present invention is to provide a grafting seedling method that can improve the disease resistance of tomatoes. The use of tomato grafting technology, management system, and rootstocks with high resistance to a variety of soil-borne diseases and tomato varieties with good yield for grafting cultivation can effectively prevent the occurrence of soil-borne diseases. This method is simple and convenient, has stronger promotion, and significantly improves the disease resistance of tomatoes and the survival rate of tomatoes.

[0008] In order to solve the above technical problems, the present invention provides a technical solution: a grafting seedling raising method capable of improving the disease resistance of tomatoes, comprising the following steps: (1) Raising seedlings of tamarillo and tomatoes: Tamarillo is germinated 10-18 days earlier than tomatoes. The method of germination is as follows: select healthy and disease-free tamarillo and tomato seeds, soak them in warm water containing plant growth regulators for a predetermined period of time; sow the soaked seeds in a hole containing seedling medium, sow one seed per hole, then cover the medium, and then wet the covered medium with seedling culture solution, and keep the temperature at 20℃-25℃; (2) Seedling management: When the tree tomato and tomato seeds are 1-3 mm white, they are transferred to the seedbed for seedling maintenance, maintaining the temperature at 20℃-25℃ and the light intensity at 300-400μmol / m 2 ·s, light duration 10-12h / d; when the seedlings grow to 6-7cm, spray growth hormone once; (3) Grafting: When the tree tomato has 4-5 true leaves, select the tree tomato as the rootstock and the tomato as the scion. Select a scion seedling with a height of 8-12 cm. Make an oblique cut 2-3 cm below the cotyledons of the scion at an angle of 45-60°. Soak the cut scion in the soaking liquid for 1-1.5 hours. At the same time, make an oblique cut at the same position and angle below the cotyledons of the rootstock. Spray the soaking liquid after cutting. Place a grafting clip of the corresponding stem thickness. Place the scion inside the grafting clip. Align and press tightly so that the oblique cut surfaces of the rootstock and scion fit together. (4) Management after grafting: Use a spray bottle to spray clean water on the grafted seedlings to keep them warm and moisturized. Keep the temperature at 20℃-25℃ and the humidity above 95%. After the scion survives after 7 days, resume the daily management of the seedlings. After the wound is completely healed, remove the grafting clips and cut off the upper part of the rootstock.

[0009] Preferably, the tree tomato seeds are local varieties from Kunming, Yunnan.

[0010] Preferably, the tomato seeds are one of Omeira 1618, Zhefen 716, Zhefen 712, Benwei, Zheza 503, and Jiahong 10.

[0011] Preferably, in the method for accelerating germination and raising seedlings, the temperature of warm water immersion is controlled at 25-30° C., and the immersion time is 48-72 hours.

[0012] Preferably, in the method for accelerating germination and raising seedlings, the plant growth regulator is gibberellin or cytokinin, and its concentration is controlled at 0.2-0.5 mg / L.

[0013] Preferably, the seedling culture medium is composed of the following components by volume: 40% vermiculite, 10% perlite, 10% peat, 10% biochar, and 30% organic fertilizer.

[0014] Preferably, the seedling culture solution comprises rooting agent 1%-5%, zinc sulfate 1%-10%, calcium oxalate 5%-12%, potassium nitrate 1%-10%, and the rest is water in percentage by weight.

[0015] Preferably, the growth hormone is an aqueous solution of 24-epi-brassinolide, and the dosage is 2000-3000 times the dilution.

[0016] Preferably, the soaking solution includes 1%-10% by weight of N,N-methylenebisacrylamide, 0.1%-0.5% of sodium nitrophenolate, 1%-3% of chitosan, 0.1%-2% of indoleacetic acid, 0.1%-2% of gibberellin, 1%-5% of fungicide, and the rest is water.

[0017] Preferably, the fungicide is 50% wettable powder of carbendazim.

[0018] Compared with the prior art, the technical solution provided by the embodiments of the present application may include the following beneficial effects: As can be seen from the above embodiments, the tree tomato in the present invention uses wild tree tomato, which has the advantages of fast growth and good disease resistance. As a rootstock, it can greatly improve the disease resistance and yield of the plant; tree tomato has a large amount of seeds, is easy to reproduce, and has a high germination rate; during the growth process, the incidence of tomatoes is high, especially the occurrence of bacterial wilt and root nematodes is serious, which causes a total crop failure in severe cases. This fundamental problem can be solved by grafting, which can resist the invasion of harmful microorganisms, improve the ability of tomato plants to tolerate adversity, and improve tomato yield and quality; the scion is soaked in a special soaking liquid, which greatly accelerates the healing speed of the grafted wound, and the healing effect is good; the optimized grafting method, higher grafting survival rate, and longer harvest period can significantly promote the green, efficient and sustainable production of crops. A grafting seedling raising method that can improve the disease resistance of tomatoes proposed by the present invention is simple and easy to implement, low cost, high yield, suitable for large-scale promotion, can greatly improve the quality and yield of tomatoes, and has significant economic and ecological benefits.

[0019] It should be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and, together with the description, serve to explain the principles of the present application.

[0021] Figure 1 It is a crop grafting schematic diagram of a grafting seedling raising method capable of improving disease resistance of tomatoes provided in Example 1 of the present invention.

[0022] Figure 2 It is a schematic diagram of non-grafted tomato seedlings in the comparative experiment provided in Example 1 of the present invention. DETAILED DESCRIPTION

[0023] The following is a description of the implementation of the present invention by means of specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0024] Embodiment 1: refer to Figure 1 , Figure 1 Schematic diagram of crop grafting of a grafting seedling raising method for improving disease resistance of tomatoes provided in Example 1 of the present invention. This embodiment provides a grafting seedling raising method for improving disease resistance of tomatoes, comprising the following steps:

[0025] (1) Raising tree tomato and tomato seedlings: First prepare the seedling matrix and seedling culture medium. The seedling matrix is ​​composed of the following components by volume: 40% vermiculite, 10% perlite, 10% peat, 10% biochar, and 30% organic fertilizer. The seedling culture medium is a mixture of 1% rooting agent, 3% zinc sulfate, 5% calcium oxalate, and 3% potassium nitrate. Tree tomato seedlings are germinated 10 days earlier than tomatoes. By adopting different sowing times, the stem thickness of the rootstock and the scion can be best matched during grafting. The method of germination seedlings is as follows: select healthy and disease-free tree tomato and Omeira 1618 tomato seeds, soak them in 30℃ warm water containing 0.2mg / L gibberellin for 48 hours to improve the water absorption capacity and germination rate of the seeds; sow the soaked seeds in a seedling matrix tray, sow 1 seed per hole, then cover the matrix, and then wet the covered matrix with seedling culture medium, keeping the temperature at 20℃;

[0026] (2) Seedling management: When the tree tomato and tomato seeds are 1mm white, transfer them to the seedbed for seedling maintenance and keep the temperature at 20°C. The light intensity is 300μmol / m 2 ·s, photoperiod 12h / d; when the seedlings grow to 6cm, spray 2000 times diluted 24-epi-brassinolide aqueous solution once;

[0027] (3) Grafting: Prepare soaking solution before grafting. The soaking solution is a mixture of 3% N,N-methylenebisacrylamide, 0.1% sodium nitrophenolate, 1% chitosan, 0.1% indoleacetic acid, 0.5% gibberellin, 1% 50% wettable powder of polysaccharides, and the rest is water. When the tree tomato has 4 true leaves, select the tree tomato as the rootstock and the tomato as the scion. Select a scion seedling with a height of 12 cm. Make an oblique cut 3 cm below the cotyledons of the scion at an angle of 45°. Soak the cut scion in the soaking solution for 1.5 hours. At the same time, make an oblique cut at the same position and angle below the cotyledons of the rootstock. Spray the soaking solution on the cut, place a grafting clip of the corresponding stem thickness, place the scion inside the grafting clip, align and fit tightly so that the oblique surfaces of the rootstock and scion fit perfectly.

[0028] (4) Management after grafting: Use a spray bottle to spray a small amount of atomized water on the grafted seedlings to keep them warm and moisturized, keeping the temperature at 25°C and the humidity above 95%. After the scion survives after 7 days, resume the daily management of the seedlings. After the wound is completely healed, remove the grafting clips and cut off the upper part of the rootstock.

[0029] Embodiment 2: This embodiment provides a grafting seedling raising method for improving disease resistance of tomatoes, comprising the following steps: (1) Raising tree tomato and tomato seedlings: First prepare the seedling matrix and seedling culture medium. The seedling matrix is ​​composed of the following components by volume: 40% vermiculite, 10% perlite, 10% peat, 10% biochar, and 30% organic fertilizer. The seedling culture medium is composed of 3% rooting agent, 10% zinc sulfate, 10% calcium oxalate, and 7% potassium nitrate, mixed with water. Tree tomato seedlings are germinated 14 days earlier than tomatoes. By adopting different sowing times, the stem thickness of the rootstock and the scion can be best matched during grafting. The method of germination and seedling raising is as follows: select healthy and disease-free tree tomato and Zhefen 716 tomato seeds, soak them in 25℃ warm water containing 0.4mg / L gibberellin for 48 hours to improve the water absorption capacity and germination rate of the seeds; sow the soaked seeds in a seedling matrix tray, sow 1 seed per hole, then cover the matrix, and then wet the covered matrix with seedling culture medium, keeping the temperature at 25℃;

[0030] (2) Seedling management: When the tree tomato and tomato seeds are 2mm white, transfer them to the seedbed for seedling maintenance and keep the temperature at 25°C. The light intensity is 300μmol / m 2 ·s, photoperiod 10h / d; when the seedlings grow to 6cm, spray 2000 times diluted 24-epi-brassinolide aqueous solution once;

[0031] (3) Grafting: Prepare soaking solution before grafting. The soaking solution is a mixture of 5% N,N-methylenebisacrylamide, 0.3% sodium nitrophenolate, 2% chitosan, 1% indoleacetic acid, 1% gibberellin, 2% 50% wettable powder of polybacterium, and the rest is water. When the tree tomato has 5 true leaves, select the tree tomato as the rootstock and the tomato as the scion. Select a scion seedling with a height of 10 cm. Make an oblique cut 3 cm below the cotyledons of the scion at an angle of 60°. Soak the cut scion in the soaking solution for 1 hour. At the same time, make an oblique cut at the same position and angle below the cotyledons of the rootstock. Spray the soaking solution on the cut, place a grafting clip of the corresponding stem thickness, place the scion inside the grafting clip, align and fit tightly so that the oblique surfaces of the rootstock and scion fit perfectly.

[0032] (4) Management after grafting: Use a spray bottle to spray a small amount of atomized water on the grafted seedlings to keep them warm and moisturized, keeping the temperature at 22°C and the humidity above 95%. After the scion survives after 7 days, resume the daily management of the seedlings. After the wound is completely healed, remove the grafting clips and cut off the upper part of the rootstock.

[0033] Embodiment three: This embodiment provides a grafting seedling raising method for improving disease resistance of tomatoes, comprising the following steps: (1) Raising tree tomato and tomato seedlings: First prepare the seedling matrix and seedling culture medium. The seedling matrix is ​​composed of the following components by volume: 40% vermiculite, 10% perlite, 10% peat, 10% biochar, and 30% organic fertilizer. The seedling culture medium is made of 5% rooting agent, 5% zinc sulfate, 12% calcium oxalate, and 1% potassium nitrate, mixed with water. Tree tomato seedlings are germinated 18 days earlier than tomatoes. By adopting different sowing times, the stem diameter of the rootstock and the scion can be best matched during grafting. The method of germination and seedling raising is as follows: select healthy and disease-free tree tomato and Zhejiang 503 tomato seeds, soak them in 25-30℃ warm water containing 0.5mg / L cytokinin for 72 hours to improve the water absorption capacity and germination rate of the seeds; sow the soaked seeds in a seedling matrix tray, sow 1 seed per hole, then cover the matrix, and then wet the covered matrix with seedling culture medium, keeping the temperature at 20℃;

[0034] (2) Seedling management: When the tree tomato and tomato seeds are 3mm white, they are transferred to the seedbed for seedling maintenance and the temperature is maintained at 25°C. The light intensity is 250 μmol / m 2 ·s, light duration 10h / d; when the seedlings grow to 6cm, spray 3000 times diluted 24-epi-brassinolide aqueous solution once;

[0035] (3) Grafting: Prepare soaking solution before grafting. The soaking solution is a mixture of 5% N,N-methylenebisacrylamide, 0.5% sodium nitrophenolate, 3% chitosan, 2% indoleacetic acid, 0.1% gibberellin, 5% 50% wettable powder of polysaccharide, and the rest is water. When the tree tomato has 5 true leaves, select the tree tomato as the rootstock and the tomato as the scion. Select a scion seedling with a height of 8 cm. Make an oblique cut 2 cm below the cotyledons of the scion at an angle of 45°. Soak the cut scion in the soaking solution for 1.5 hours. At the same time, make an oblique cut at the same position and angle below the cotyledons of the rootstock. Spray the soaking solution on the cut, place a grafting clip of the corresponding stem thickness, place the scion inside the grafting clip, align and fit tightly so that the oblique surfaces of the rootstock and scion fit perfectly.

[0036] (4) Management after grafting: Use a spray bottle to spray a small amount of atomized water on the grafted seedlings to keep them warm and moisturized, keeping the temperature at 20°C and the humidity above 95%. After the scion survives after 7 days, resume the daily management of the seedlings. After the wound is completely healed, remove the grafting clips and cut off the upper part of the rootstock.

[0037] Embodiment 4: This embodiment provides a grafting seedling raising method for improving disease resistance of tomatoes, comprising the following steps: (1) Raising tree tomato and tomato seedlings: First prepare the seedling matrix and seedling culture medium. The seedling matrix is ​​composed of the following components by volume: 40% vermiculite, 10% perlite, 10% peat, 10% biochar, and 30% organic fertilizer. The seedling culture medium is a mixture of 5% rooting agent, 1% zinc sulfate, 12% calcium oxalate, and 1% potassium nitrate. Tree tomato seedlings are germinated 14 days earlier than tomatoes. By adopting different sowing times, the stem thickness of the rootstock and the scion can be best matched during grafting. The method of germination seedlings is as follows: select healthy, disease-free tree tomato and Jiahong 10 tomato seeds, soak them in 25-30℃ warm water containing 0.5mg / L cytokinin for 72 hours to improve the water absorption capacity and germination rate of the seeds; sow the soaked seeds in a seedling matrix tray, sow 1 seed per hole, then cover the matrix, and then wet the covered matrix with seedling culture medium, keeping the temperature at 25℃;

[0038] (2) Seedling management: When the tree tomato and tomato seeds are 3mm white, they are transferred to the seedbed for seedling maintenance, and the temperature is maintained at 25°C. The light intensity is 400μmol / m 2 ·s, light duration 12h / d; when the seedlings grow to 7cm, spray 3000 times diluted 24-epi-brassinolide aqueous solution once;

[0039] (3) Grafting: Prepare soaking solution before grafting. The soaking solution is a mixture of 1% N,N-methylenebisacrylamide, 0.5% sodium nitrophenolate, 3% chitosan, 2% indoleacetic acid, 0.1% gibberellin, 5% 50% wettable powder of polysaccharide, and the rest is water. When the tree tomato has 5 true leaves, select the tree tomato as the rootstock and the tomato as the scion. Select a scion seedling with a height of 8 cm. Make an oblique cut 2 cm below the cotyledons of the scion at an angle of 60°. Soak the cut scion in the soaking solution for 1 hour. At the same time, make an oblique cut at the same position and angle below the cotyledons of the rootstock. Spray the soaking solution on the cut, place a grafting clip of the corresponding stem thickness, place the scion inside the grafting clip, align and fit tightly so that the oblique surfaces of the rootstock and scion fit perfectly.

[0040] (4) Management after grafting: Use a spray bottle to spray a small amount of atomized water on the grafted seedlings to keep them warm and moisturized, keeping the temperature at 25°C and the humidity above 95%. After the scion survives after 7 days, resume the daily management of the seedlings. After the wound is completely healed, remove the grafting clips and cut off the upper part of the rootstock.

[0041] Comparative experiment: A comparative experiment was conducted with non-grafted tomatoes as the control group and Example 1 as the experimental group. Three weeks after grafting, the grafted seedlings of Example 1 and the non-grafted tomato seedlings were moved into a climate chamber laboratory, and 20 g of Pseudomonas solanacearum cultured with barley grains was mixed into the soil at the bottom of the transplanting hole. Six plants were planted in each group and repeated three times. The wilt condition and growth were investigated 30 days after planting, and the weight of a single fruit was calculated 60 days after planting. The results are shown in the following table.

[0042] project Incidence / % Bacterial wilt control effect / % Average single fruit weight / g <![CDATA[Sugar content / 。 Bx]]> Non-grafted tomatoes 60 - 141.18 4.6 Embodiment 1 2 98 154.47 4.9

[0043] from Figure 1 and Figure 2 It can be seen that the stem thickness of the grafted seedlings with tree tomato plants as rootstocks and tomatoes as scion is significantly thicker than that of the control group of non-grafted tomatoes. The developed root system and thick stems of the tree tomato rootstock can better provide nutrients and water for the tomato scion, thereby improving the survival rate of the grafted tomato seedlings.

[0044] As can be seen from the above table, the present invention uses tree tomato plants as rootstocks and tomatoes as scion varieties, which significantly reduces the incidence of bacterial wilt disease, thereby increasing the survival rate of grafted tomato seedlings, and at the same time increases the single fruit weight and sugar content of tomatoes.

[0045] Those skilled in the art will readily appreciate other embodiments of the present application after considering the description and practicing the contents disclosed herein. The present application is intended to cover any modification, use or adaptation of the present application, which follows the general principles of the present application and includes common knowledge or customary techniques in the art that are not disclosed in the present application. The description and examples are intended to be exemplary only, and the true scope and spirit of the present application are indicated by the claims.

[0046] It should be understood that the present application is not limited to what has been described above, and that various modifications and changes may be made without departing from its scope. The scope of the present application is limited only by the appended claims.

Claims

1. A grafting seedling raising method for improving disease resistance of tomatoes, characterized in that: The following steps are involved: (1) Raising seedlings of tamarillo and tomatoes: Tamarillo is germinated 10-18 days earlier than tomatoes. The method of germination is as follows: select healthy and disease-free tamarillo and tomato seeds, soak them in warm water containing plant growth regulators for a predetermined period of time; sow the soaked seeds in a hole containing seedling medium, sow one seed per hole, then cover the medium, and then wet the covered medium with seedling culture solution, and keep the temperature at 20℃-25℃; (2) Seedling management: When the tree tomato and tomato seeds are 1-3 mm white, they are transferred to the seedbed for seedling maintenance, maintaining the temperature at 20℃-25℃ and the light intensity at 300-400μmol / m 2 ·s, light duration 10-12h / d; when the seedlings grow to 6-7cm, spray growth hormone once; (3) Grafting: When the tree tomato has 4-5 true leaves, select the tree tomato as the rootstock and the tomato as the scion. Select a scion seedling with a height of 8-12 cm. Make an oblique cut 2-3 cm below the cotyledons of the scion at an angle of 45-60°. Soak the cut scion in the soaking liquid for 1-1.5 hours. At the same time, make an oblique cut at the same position and angle below the cotyledons of the rootstock. Spray the soaking liquid after cutting. Place a grafting clip of the corresponding stem thickness. Place the scion inside the grafting clip. Align and press tightly so that the oblique cut surfaces of the rootstock and scion fit together. (4) Management after grafting: Use a spray bottle to spray clean water on the grafted seedlings to keep them warm and moisturized. Keep the temperature at 20℃-25℃ and the humidity above 95%. After the scion survives after 7 days, resume the daily management of the seedlings. After the wound is completely healed, remove the grafting clips and cut off the upper part of the rootstock.

2. A grafting seedling raising method for improving disease resistance of tomatoes according to claim 1, characterized in that: The tree tomato seeds are a local variety in Kunming, Yunnan.

3. A grafting seedling raising method for improving disease resistance of tomatoes according to claim 1, characterized in that: The tomato seeds are one of Omeira 1618, Zhefen 716, Zhefen 712, Benwei, Zheza 503, and Jiahong 10.

4. A grafting seedling raising method for improving disease resistance of tomatoes according to claim 1, characterized in that: In the method for accelerating germination and raising seedlings, the temperature of warm water soaking is controlled at 25-30° C., and the soaking time is 48-72 hours.

5. The grafting seedling raising method for improving disease resistance of tomatoes according to claim 1, characterized in that: In the method for accelerating germination and raising seedlings, the plant growth regulator is gibberellin or cytokinin, and the concentration of the plant growth regulator is controlled at 0.2-0.5 mg / L.

6. The grafting seedling raising method for improving disease resistance of tomatoes according to claim 1, characterized in that: The seedling culture medium is composed of the following components by volume: 40% of vermiculite, 10% of perlite, 10% of peat, 10% of biochar and 30% of organic fertilizer.

7. A grafting seedling raising method for improving disease resistance of tomatoes according to claim 1, characterized in that: The seedling culture solution comprises rooting agent 1%-5%, zinc sulfate 1%-10%, calcium oxalate 5%-12%, potassium nitrate 1%-10%, and the rest is water in percentage by weight.

8. The grafting seedling raising method for improving disease resistance of tomatoes according to claim 1, characterized in that: The growth hormone is an aqueous solution of 24-epi-brassinolide, and the dosage is 2000-3000 times.

9. A grafting seedling raising method for improving disease resistance of tomatoes according to claim 1, characterized in that: The soaking liquid comprises 1%-10% by weight of N,N-methylenebisacrylamide, 0.1%-0.5% of sodium nitrophenolate, 1%-3% of chitosan, 0.1%-2% of indoleacetic acid, 0.1%-2% of gibberellin, 1%-5% of bactericide, and the rest is water.

10. A grafting seedling raising method for improving disease resistance of tomatoes according to claim 9, characterized in that: The fungicide is 50% wettable powder of carbendazim.

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