A hair repair composition, its preparation method and application

Abstract

This invention discloses a hair repair composition, its preparation method, and its application, relating to the field of daily chemical products technology. The hair repair composition provided by this invention comprises 0.1–0.3 parts of *Dendrobium officinale* extract, 0.15–0.4 parts of *Tremella fuciformis* extract, 10–60 parts of *Galactomyces* fermentation product filtrate, and 39.3–89.75 parts of *Bifida fragrans* fermentation product filtrate. Utilizing the synergistic effect among the four components, a polysaccharide biofilm is formed on the hair surface, preventing moisture loss from the hair cuticle and absorbing moisture from the air or solvents, thus closing the hair cuticles. Simultaneously, the active substances abundant in the four components can penetrate deep into the hair shaft to nourish it. Therefore, the hair repair composition provided by this invention can improve hair shine, reduce frizz, and reduce the effort required to comb dry and wet hair.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products technology, specifically to a hair repair composition, its preparation method, and its application. Background Technology

[0002] Hair consists of three parts: the hair root, the hair follicle, and the hair shaft. The hair shaft, or hair strand, comprises three parts: the cuticle, the cortex, and the medulla. The cuticle is the outermost layer of the hair fiber, composed of flattened cells arranged in a unidirectional, fish-scale-like structure called "hair scales." The hair scales of the cuticle play a vital protective role. Once the hair structure is damaged, the cuticle is the most vulnerable and the first part to be affected. Studies have shown that the hair scales of healthy hair are tightly and neatly arranged, while in hair that has been permed once, some hair scales are rolled up and "open"; in hair that has been permed two or three times, most of the hair scales are rolled up and fall off, indicating severe damage to the hair cuticle and a decline in the integrity of the hair scale structure.

[0003] Numerous factors can cause damage to hair structure. In addition to perming as mentioned earlier, internal and external factors such as stress, air pollution, and microorganisms can also affect the integrity of hair structure. Damage to hair structure can involve morphological changes on the hair surface (such as curling, split ends, etc.), changes in color and shine, and even changes in hair combability. These changes are all caused by severe damage to the cuticle layer, resulting in the "opening" or shedding of the tightly packed, one-way hair cuticles.

[0004] Damage to hair structure can cause many problems, such as difficulty in combing hair and affecting one's appearance. Therefore, providing a hair repair composition that can preserve the integrity of the hair cuticle structure and thus improve hair shine and combability has become one of the urgent problems to be solved. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a hair repair composition that utilizes the synergistic effect of four components—Dendrobium officinale extract, Tremella fuciformis extract, Galactomyces ferment filtrate, and Bifida ferment filtrate—to repair damaged hair cuticles and improve hair shine and combability.

[0006] Another object of the present invention is to provide a method for preparing a hair repair composition.

[0007] Another object of the present invention is to provide a hair care product.

[0008] The above-mentioned objective of this invention is achieved through the following technical solution:

[0009] A hair repair composition comprising the following components in parts by weight:

[0010] 0.1–0.3 parts Dendrobium officinale extract, 0.15–0.4 parts Tremella fuciformis extract, 10–60 parts filtrate of galactosomal yeast fermentation product, and 39.3–89.75 parts filtrate of Bifida ferment lysate fermentation product.

[0011] The hair repair composition provided by this invention includes Dendrobium officinale extract, which is rich in Dendrobium officinale polysaccharides and flavonoids. Dendrobium officinale polysaccharides have excellent moisturizing effects, while flavonoids have excellent antioxidant effects. Applying Dendrobium officinale extract to the hair surface can improve frizz, enhance hair combability, and repair hair cuticles. Tremella fuciformis Berk extract is rich in active substances such as Tremella fuciformis polysaccharides. Tremella fuciformis polysaccharides are rich in polar groups such as hydroxyl and carboxyl groups, which can bind a large amount of water, resulting in a final product that exhibits excellent hair repair effects. The filtrate of Galactomyces fermentation product is obtained from the fermentation of Mycosaccharomyces cerevisiae and is rich in vitamins, minerals, amino acids, organic acids, enzymes, and yeast peptides, possessing excellent moisturizing effects. Bifida Ferment Lysate is a fermentation product filtrate obtained after processing Bifidobacterium fermentation. It is rich in vitamins, minerals, amino acids, polysaccharides, peptides, and other active small molecules, thus exhibiting certain moisturizing, antioxidant, and anti-aging effects. It can also promote the repair of damaged DNA. These advantages allow Bifida Ferment Lysate to be used in skincare products to improve skin texture. Based on the above-mentioned functions of Bifida Ferment Lysate, this invention incorporates it into hair repair compositions to achieve better hair repair effects in the final product.

[0012] After mixing the above four components to form the hair repair composition of this invention, the Dendrobium officinale polysaccharide in the Dendrobium officinale extract and the Tremella fuciformis polysaccharide in the Tremella fuciformis extract can form a cross-network structure through hydrogen bonding and electrostatic interactions, forming a bio-polysaccharide film on the surface of the hair. This not only acts as a sealant to prevent moisture loss from the hair cuticle, but also absorbs moisture from the air or solvent through the hydroxyl and carboxyl groups of the polysaccharides, closing the hair cuticle, reducing frizz, and making the hair soft and smooth. At the same time, the four components in the composition of this invention are rich in amino acids, polypeptides, vitamins, and other active substances. These active substances can penetrate the cuticle layer and reach deep into the hair cortex, effectively replenishing the nutrients needed by damaged hair and nourishing the hair from the inside. In addition, Dendrobium officinale extract is rich in flavonoids, which can reduce oxidative damage to hair, reduce the breakage of disulfide bonds, degradation of amino acids and oxidation of lipids in the epidermis, and reduce problems such as roughness, decreased toughness and reduced mechanical properties of hair. In summary, the composition formed by combining Dendrobium officinale extract, Tremella fuciformis extract, galactosomal yeast fermentation product filtrate and Bifida ferment filtrate of this invention can synergistically repair hair from the inside out.

[0013] Preferably, the Dendrobium officinale extract is prepared by enzymatic extraction of Dendrobium officinale using immobilized enzymes;

[0014] The tremella extract was prepared by fermenting and extracting tremella with Candida utilis.

[0015] The Tremella fuciformis extract in the composition provided by the present invention is preferably prepared by fermentation extraction using Candida utilis, and the Tremella fuciformis polysaccharide in the obtained extract has an ultra-low molecular weight. Tremella fuciformis extracts prepared by other methods have higher molecular weights of Tremella fuciformis polysaccharides, resulting in a decreased hair repair effect. At the same time, the Dendrobium officinale extract used in the present invention is also preferably obtained by enzymatic hydrolysis using immobilized enzymes, thereby obtaining Dendrobium officinale polysaccharides and flavonoids in the obtained extract with even lower molecular weights.

[0016] Preferably, the hair repair composition comprises the following components in parts by weight:

[0017] 0.13–0.16 parts of Dendrobium officinale extract, 0.25–0.3 parts of Tremella fuciformis extract, 28–43 parts of filtrate of fermentation product of Galactomyces-like bacteria, and 56–72 parts of filtrate of fermentation product of Bifida ferment lysate.

[0018] The inventors' experiments have confirmed that when the proportions of each component are within the above-mentioned range, the resulting composition has a superior hair repair effect.

[0019] Preferably, the immobilized enzyme comprises an immobilized enzyme carrier and an enzyme loaded on the immobilized enzyme carrier, wherein the enzyme comprises at least one of Aspergillus niger acid β-mannanase, cellulase, a product enzyme of Bacillus noubii anaerobicus, and a neutral protease.

[0020] Preferably, the preparation method of the Dendrobium officinale extract includes the following steps:

[0021] Dendrobium officinale stem powder was dispersed in water to obtain a solution, which was then heated at 60-80℃ for water extraction. After water extraction, immobilized enzymes were added to enzymatically hydrolyze the Dendrobium officinale to obtain Dendrobium officinale extract.

[0022] More preferably, the mass ratio of the Dendrobium officinale stem powder to water is 1:(20-40).

[0023] The inventors' experiments have confirmed that when the mass ratio of Dendrobium officinale stem powder to water is within the above-mentioned range, the resulting composition has a superior hair repair effect.

[0024] In a specific embodiment of the present invention, the pH of the solution in the water extract is 6.0-6.8.

[0025] In a specific embodiment of the present invention, the heating treatment time in the water extraction is 20-40 minutes.

[0026] In a specific embodiment of the present invention, the method for preparing the immobilized enzyme is carried out in accordance with Chinese Patent CN117106764A.

[0027] In a specific embodiment of the present invention, the amount of the immobilized enzyme added is 3-8% of the mass of Dendrobium officinale stem powder.

[0028] In a specific embodiment of the present invention, the enzymatic hydrolysis temperature is 60-70°C and the enzymatic hydrolysis time is 80-160 min.

[0029] In a specific embodiment of the present invention, the enzymatic hydrolysis is further followed by filtration and sterilization steps.

[0030] In a specific embodiment of the present invention, the sterilization temperature is 80-95°C and the sterilization time is 15-30 minutes.

[0031] Preferably, the tremella extract uses a bacterial concentration of 10... 7 -10 9 The *C. utilis* seed culture was fermented and extracted from *Tremella fuciformis* to obtain the *Tremella fuciformis* seed culture. The mass ratio of the *C. utilis* seed culture to *Tremella fuciformis* was (2-5):(0.5-1.8).

[0032] In a specific embodiment of the present invention, the fermentation and extraction of Tremella fuciformis using Candida utilis involves inoculating the seed culture of Candida utilis into the Tremella fuciformis fermentation medium for fermentation, thereby obtaining the Tremella fuciformis extract.

[0033] More specifically, the fermentation temperature is 28-33℃, and the fermentation time is 40-60h.

[0034] More specifically, the fermentation is carried out under stirring conditions at a speed of 100-200 rpm.

[0035] More specifically, the tremella culture medium comprises 0.5-1.8% tremella, 1-4% carbon source, 0.3-1.2% nitrogen source and 0.35-0.8% inorganic salts, with the balance being water.

[0036] More specifically, the tremella includes at least one of Tongjiang tremella, Gutian tremella, Guizhou tremella, and Yunnan tremella; the carbon source includes at least one of fructose syrup, dextrin, soybean starch, potato starch, and molasses; the nitrogen source includes at least one of soybean peptone, yeast extract, cottonseed meal, and tryptone; and the inorganic salt includes sulfate and / or phosphate.

[0037] More specifically, the fermentation extraction process also includes sterilization and filtration steps.

[0038] Preferably, the sterilization temperature is 80-95℃ and the sterilization time is 15-30 minutes.

[0039] This invention also protects a method for preparing a hair repair composition, comprising the following steps:

[0040] A hair repair composition can be obtained by mixing Dendrobium officinale extract, Tremella fuciformis extract, galactosomal yeast fermentation product filtrate, and Bifida ferment lysate fermentation product filtrate.

[0041] The present invention also protects a hair care product comprising the above-described hair repair composition.

[0042] The hair care product described in this invention is any formulation conventionally prepared in the field of hair care products, and can be formulated into at least one of the following: solution, suspension, emulsion, paste, gel, cream, water, powder, soap, surfactant-containing detergent, oil, powder cream, and spray.

[0043] In a specific embodiment of the present invention, the hair care product of the present invention includes at least one of the following: hair conditioner, hair nourishing cream, hair massage cream, hair essence, cleansing cream, cleansing foam, cleansing water, hair moisturizing cream, shampoo, and conditioner.

[0044] In a specific embodiment of the present invention, the hair repair composition accounts for 0.1-100% of the weight of the hair care product.

[0045] Compared with the prior art, the present invention has the following beneficial effects:

[0046] The hair repair composition provided by this invention can form a biopolysaccharide film on the hair surface, exhibiting excellent moisture absorption and retention effects and repairing the hair cuticle structure. Using the composition provided by this invention to repair hair can increase hair shine by more than 3%, reduce combing effort in dry and wet hair by 3.79% and 29.57% respectively, and significantly improve hair smoothness. Attached Figure Description

[0047] Figure 1 Scanning electron microscope image of the hair strands in the blank control group during the hair cuticle repair test.

[0048] Figure 2 Scanning electron microscope (SEM) images of hair strands from the test group in Example 1 during the hair cuticle repair test.

[0049] Figure 3 Scanning electron microscope (SEM) images of hair strands from the test group in Example 2 during the hair cuticle repair test.

[0050] Figure 4 Scanning electron microscope (SEM) images of hair strands from the test group in Example 3 during the hair cuticle repair test.

[0051] Figure 5 Scanning electron microscope (SEM) images of hair strands from test group 4 in Example 4 during the hair cuticle repair test. Detailed Implementation

[0052] The present invention will be further described below with reference to specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise stated, all percentages and inoculation amounts mentioned in the present invention are mass percentages. Unless otherwise stated, the raw materials and reagents used in the embodiments of the present invention are conventionally purchased raw materials and reagents.

[0053] The raw material information used in each embodiment and comparative example is as follows:

[0054] Galactomyces fermentation product filtrate: purchased from Guangzhou Giant Microbial Technology Co., Ltd., product number GalaFF.

[0055] Bifida ferment filtrate: purchased from Perfect (Guangdong) Daily Necessities Co., Ltd., material code C00817.

[0056] Dendrobium officinale extract: prepared using the following method:

[0057] Dendrobium officinale stem powder was dispersed in 30 times its weight of water to obtain a solution. The pH of the solution was adjusted to 6.5 with 1 wt% hydrochloric acid solution, and the solution was heated at 70°C for 30 min to obtain a suspension. Immobilized enzyme at 5% by weight of Dendrobium officinale stem powder was added to the suspension, and enzymatic hydrolysis was performed at 65°C for 120 min. The solution was filtered through a diatomaceous earth filter, and the filtrate was sterilized by incubating at 90°C for 20 min to obtain Dendrobium officinale extract. The preparation method of the immobilized enzyme was carried out according to Example 1 in Chinese Patent CN117106764A.

[0058] Tremella fuciformis extract: prepared using the following method:

[0059] The bacterial concentration was 10. 8 CFU / mL *Candida utilis* seed culture was inoculated into *Tremella fuciformis* fermentation medium at a 3% inoculum. Fermentation was carried out at 30℃ and a shaking speed of 150 rpm for 50 h to prepare crude fermentation broth. The broth was sterilized by incubation at 90℃ for 20 min, cooled, and filtered through a microfiltration membrane to obtain *Tremella fuciformis* extract. The formulation of the *Tremella fuciformis* fermentation medium was: 1.2% *Tremella fuciformis* powder, 2% fructose syrup, 0.8% soybean peptone, 0.3% magnesium sulfate, 0.15% potassium dihydrogen phosphate, 0.1% dipotassium hydrogen phosphate, and the remainder water.

[0060] Candida utilis: Candida utilis GDMCC2.148, purchased from Guangdong Provincial Microbial Culture Collection Center.

[0061] The Ganoderma lucidum extract, brown algae extract, yeast fermentation product extract, and lactic acid bacteria fermentation extract used in this invention were all purchased from Dongguan Juwei New Materials Technology Co., Ltd.

[0062] Examples 1-6 and Comparative Examples 1-11

[0063] This embodiment and the comparative example provide a series of hair repair compositions with different component mass parts, as shown in Table 1 below:

[0064] Table 1. Mass fractions of each component in Examples 1-6 and Comparative Examples 1-11

[0065]

[0066]

[0067] Comparative Example 12

[0068] A hair repair composition, wherein the only difference from Example 2 is:

[0069] In the preparation method of Dendrobium officinale extract, enzymatic hydrolysis is carried out using 3% (by weight) cellulase and 2% (by weight) pectinase of Dendrobium officinale stem powder.

[0070] Comparative Example 13

[0071] A hair repair composition, wherein the only difference from Example 2 is:

[0072] The extract of Dendrobium officinale was prepared by fermentation extraction of Dendrobium officinale using Candida utilis. The specific preparation method includes the following steps:

[0073] The bacterial concentration was 10. 8 CFU / mL Candida utilis seed culture was inoculated into Dendrobium officinale fermentation medium at a 3% inoculation rate. Fermentation was carried out at 30℃ and the shaking speed of the fermentation flask was 150 rpm for 50 h to prepare crude fermentation broth. After incubation at 90℃ for 20 min, and cooling, Dendrobium officinale extract was obtained by microfiltration.

[0074] The formula for the Dendrobium officinale fermentation medium is as follows: 1.2% Dendrobium officinale stem powder, 2% fructose syrup, 0.8% soybean peptone, 0.3% magnesium sulfate, 0.15% potassium dihydrogen phosphate, 0.1% dipotassium hydrogen phosphate, and the balance being water.

[0075] Comparative Example 14

[0076] A hair repair composition, wherein the only difference from Example 2 is:

[0077] The tremella extract was prepared by fermenting tremella with Saccharomyces cerevisiae. The specific fermentation and extraction method was consistent with that in Example 2, which used Candida utilis for fermentation and extraction. The only difference was that the Candida utilis seed culture was replaced with the Saccharomyces cerevisiae seed culture (concentration of 10) during fermentation. 8 (CFU / mL).

[0078] The Saccharomyces cerevisiae used in this comparative example is Saccharomyces cerevisiae BNCC336054, purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0079] Comparative Example 15

[0080] A hair repair composition, wherein the only difference from Example 2 is:

[0081] The process of obtaining Tremella fuciformis extract includes the following steps:

[0082] Tongjiang white fungus powder was dispersed in 30 times its weight of water to obtain a solution. The pH of the solution was adjusted to 6.5 with 1 wt% hydrochloric acid solution, and the solution was heated at 70°C for 30 min to obtain a suspension. Immobilized enzyme of 5% by weight of Tongjiang white fungus powder was added to the suspension, and enzymatic hydrolysis was carried out at 65°C for 120 min. The solution was then filtered through a diatomaceous earth filter, and the filtrate was sterilized by incubation at 90°C for 20 min to obtain white fungus extract. The preparation method of the immobilized enzyme was carried out according to Example 1 in Chinese Patent CN117106764A.

[0083] Comparative Example 16

[0084] A hair repair composition, wherein the only difference from Example 2 is:

[0085] Replace the Dendrobium officinale extract with an equivalent amount of Ganoderma lucidum extract.

[0086] Comparative Example 17

[0087] A hair repair composition, wherein the only difference from Example 2 is:

[0088] Replace the tremella extract with an equal amount of brown algae extract.

[0089] Comparative Example 18

[0090] A hair repair composition, wherein the only difference from Example 2 is:

[0091] Replace the filtrate of galactosomal yeast fermentation product with an equal mass of yeast fermentation product extract.

[0092] Comparative Example 19

[0093] A hair repair composition, wherein the only difference from Example 2 is:

[0094] Replace the Bifida ferment filtrate with an equal mass of lactic acid bacteria ferment extract.

[0095] Performance testing

[0096] The compositions obtained in Examples 1-6 and Comparative Examples 1-19 were tested to investigate their stability, hair repair effects, and other properties.

[0097] I. Stability Testing

[0098] Test samples: hair repair compositions of Examples 1-6 and Comparative Examples 1-19.

[0099] Test procedure: Take 15 mL of the test sample and place it in a colorless transparent PE sample bottle. Seal the sample and place it at 25°C for 3 weeks. Test the conductivity, pH and viscosity of the sample at week 0 and week 3, respectively.

[0100] Testing instruments:

[0101] Conductivity: Conductivity meter, Leici DDBJ-350.

[0102] pH: pH meter, SevenCompact S210-S.

[0103] Viscosity: Rotational viscometer, IKA ROTAVISC ME-VI S000.

[0104] The test results are shown in Table 2 below.

[0105] Table 2. Stability data of the compositions obtained in the examples and comparative examples

[0106]

[0107]

[0108]

[0109] Test results show that the hair repair compositions of Examples 1-6 of this invention have good stability. After being placed at room temperature for 3 weeks, their conductivity, pH, and viscosity did not change significantly, thus demonstrating their potential for large-scale commercial production. In contrast, some comparative examples of hair repair compositions showed significant changes in conductivity, pH, and viscosity after being placed at room temperature for 3 weeks, indicating poor stability. Specifically, the hair compositions of Comparative Examples 1 and 2 lacked one of the following extracts: Dendrobium officinale extract and Tremella fuciformis extract. Comparative Examples 16 and 17 replaced Dendrobium officinale extract and Tremella fuciformis extract with similar plant extracts, respectively. The resulting hair compositions showed significant changes in conductivity, pH, and viscosity, indicating that Dendrobium officinale extract and Tremella fuciformis extract play important roles in maintaining the stability of the system.

[0110] II. Hair Shine Test

[0111] Test samples: hair repair compositions of Examples 1-6 and Comparative Examples 1-19.

[0112] Test steps:

[0113] (1) Hair strand pretreatment: Prepare 75 damaged hair strands and randomly divide them into 25 groups of 3 hair strands each. Before testing, clean the hair strands with a 3% SDS aqueous solution and let them air dry at room temperature.

[0114] (2) Sample use: Apply 1.5 mL of test sample gently and evenly to the upper, middle and lower sections of the hair strand, let it stand to dry, then wash the hair strand with deionized water, and let it dry before testing.

[0115] (3) Glossiness Test: After the hair strand pretreatment is completed and the sample is used, the hair strand is fixed with a test clip, and the glossiness of the hair strand surface is tested through a skin glossiness probe. The relevant data are recorded and the change rate of hair glossiness before and after using the test sample is calculated. Each sample is tested in parallel 3 times, and the average value of the test results is taken. The testing instrument is a skin glossiness probe Glossymeter GL200.

[0116] Hair gloss change rate (%) = (hair strand gloss after using test sample - hair strand gloss after pretreatment) / hair strand gloss after pretreatment × 100%.

[0117] When the rate of change in hair shine is positive, it indicates that the test sample has the effect of brightening the hair.

[0118] The test results are shown in Table 3 below:

[0119] Table 3. Gloss test data of the examples and comparative examples

[0120]

[0121]

[0122] Test results show that the hair repair composition of this invention has a good effect on brightening hair shine. After using the hair repair composition of this invention, the shine of the hair increased by more than 3%, with a maximum of 34.59%. Among them, the shine change rate of Example 2 is much higher than that of Comparative Examples 1-19, indicating that only the Dendrobium officinale extract and Tremella fuciformis extract obtained by the extraction method of this invention can have a synergistic effect with the filtrate of Galactomyces-like Ferment and Bifida Ferment. The absence of any component (Comparative Examples 1-5) will lead to a decrease in the composition's ability to improve hair shine. Using other extraction methods to prepare the two plant extracts of this invention (Comparative Examples 12-15) will also lead to a decrease in the synergistic effect. Replacing any of the four components with other components with similar functions (Comparative Examples 16-19) will also lead to a loss of the synergistic effect. At the same time, the comparison of the data of the examples with Comparative Examples 6-11 shows that the mass ratio of each component in the composition provided by this invention should be within an appropriate range to ensure the maximum synergistic effect.

[0123] III. Dry Hair Combing Performance Test

[0124] Test samples: hair repair compositions of Examples 1-6 and Comparative Examples 1-19.

[0125] Test steps:

[0126] (1) Hair strand pretreatment: 78 damaged hair strands were prepared and randomly divided into 26 groups of 3 hair strands each. Before testing, the hair strands were cleaned with a 3% SDS aqueous solution and air-dried at room temperature for later use.

[0127] (2) Sample application: Apply 1.5 mL of test sample gently and evenly to the upper, middle and lower sections of the hair strand, rinse with tap water while smoothing the hair, rinse thoroughly, and air dry at room temperature before testing. The blank control group does not apply the sample, but washes the hair strand with tap water along with the sample group, and air dry before testing.

[0128] (3) Dry hair combing ability test: After the hair strand pretreatment is completed and the sample is used, the hair strand is fixed with a test clip and the combing ability test is carried out. The test is repeated 5 times. Each test sample is tested in parallel with 3 hair strands. The relevant data are recorded and the rate of change of combing ability of the hair strand before and after using the test sample is calculated. The average value of the test results is taken. The test instrument is MTT175 hair tester (Tress Volume Accessory).

[0129] Change rate of dry hair combing effort (%) = (combing effort of hair strand after using test sample - combing effort of hair strand after pretreatment) / combing effort of hair strand after pretreatment × 100.

[0130] The smaller the combing effort of the hair strand, the easier the hair strand is to comb. That is, when the change rate of dry hair combing effort is negative, it indicates that the test sample has the effect of smoothing hair strands.

[0131] IV. Wet Hair Combing Performance Test

[0132] Test samples: hair repair compositions of Examples 1-6 and Comparative Examples 1-19.

[0133] Test steps:

[0134] (1) Hair strand pretreatment: 78 damaged hair strands were prepared and randomly divided into 26 groups of 3 hair strands each. Before testing, the hair strands were cleaned with a 3% SDS aqueous solution and air-dried at room temperature for later use.

[0135] (2) Sample application: Gently and evenly apply 1.5 mL of test sample to the upper, middle and lower sections of the hair strand, rinse with tap water while smoothing the hair, and rinse thoroughly. The blank control group does not apply the sample, and washes the hair strand with tap water together with the sample group, and then dries before testing.

[0136] (3) Wet hair combing ability test: After the hair bundle pretreatment, the hair bundle was soaked in clean water, combed, gently kneaded, and then rinsed. It was then clipped onto the test clip for a wet hair combing ability test, repeated 5 times, and the wet hair combing ability before use was recorded. After use, the sample was rinsed clean with water, then clipped onto the test clip for a wet hair combing ability test, repeated 5 times, and the wet hair combing ability after use was recorded. Each test sample was tested in parallel with 3 hair bundles. For each group, relevant data were recorded, and the rate of change in combing ability of the hair bundle before and after using the test sample was calculated. The average value of the test results was taken. The testing instrument was an MTT175 hair tester (Tress Volume Accessory).

[0137] Change rate of wet hair combing effort (%) = (combing effort of hair strand after using test sample - combing effort of hair strand after pretreatment) / combing effort of hair strand after pretreatment × 100.

[0138] The smaller the combing effort of the hair strand, the easier the hair strand is to comb. That is, when the change rate of the combing effort of wet hair is negative, it indicates that the test sample has the effect of smoothing hair strands.

[0139] The results of the above tests on the combability of dry and wet hair are shown in Table 4 below:

[0140] Table 4. Test data on combability of dry and wet hair in the examples and comparative examples

[0141]

[0142]

[0143] Hair smoothness is a commonly used parameter for evaluating the effectiveness of hair products. When hair is damaged by physical or chemical treatments, the loss of liposomes and the absence or lifting of hair cuticles lead to a rougher hair surface, increasing friction between hair strands and reducing smoothness. Hair smoothness can be quantified by measuring the combing force or work done when a comb passes through the hair in both wet and dry states; this is commonly referred to as dry combing and wet combing.

[0144] Test results show that, compared with the blank group, the hair strands were repaired after using the hair repair composition of the present invention. Furthermore, the change rate of hair combing ability was lower than that of the blank control group. The change rates of dry hair combing ability and wet hair combing ability in all embodiments were negative, indicating that the hair became easier to comb after using the samples of Examples 1-6.

[0145] V. Cuticle Repair Test

[0146] Test samples: hair repair compositions of Examples 1-4.

[0147] Test Procedure: Prepare 5 damaged hair bundles. Before testing, clean the hair bundles with a 3% SDS aqueous solution and air dry at room temperature. Immerse the hair bundles in the test sample and deionized water (blank control group) respectively for 10 minutes. After immersion, remove the hair bundles and air dry at room temperature, then photograph them using a scanning electron microscope. Test results are as follows: Figures 1 to 5 As shown. Figure 1 As shown, when hair is damaged, the hair cuticles on its surface exhibit problems such as lifting, breakage, and wrinkling. After using the hair repair composition of the embodiments of the present invention, the lifting of the hair cuticles was improved, and the hair cuticles closed, indicating that the hair repair composition of the present invention has the effect of repairing the integrity of the hair cuticle structure. (Comparison) Figure 2-5 It is evident that the hair repair composition of Example 2 has the best effect on repairing hair cuticles.

[0148] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A hair repair composition, characterized in that, It consists of the following components, calculated in parts by mass: 0.1-0.3 parts Dendrobium officinale extract, 0.15-0.4 parts Tremella fuciformis extract, 10-60 parts filtrate of Galactomyces fermentation product, and 39.3-89.75 parts filtrate of Bifida ferment lysate; The Dendrobium officinale extract was prepared by enzymatic hydrolysis of Dendrobium officinale using immobilized enzymes; the Tremella fuciformis extract was prepared by fermentation extraction of Tremella fuciformis using Candida utilis.

2. The hair repair composition as described in claim 1, characterized in that, It consists of the following components, calculated in parts by mass: 0.13-0.16 parts Dendrobium officinale extract, 0.25-0.3 parts Tremella fuciformis extract, 28-43 parts filtrate of galactosomal yeast fermentation product, and 56-72 parts filtrate of Bifida ferment lysate fermentation product.

3. The hair repair composition as described in claim 1, characterized in that, The immobilized enzyme includes an immobilized enzyme carrier and an enzyme loaded on the immobilized enzyme carrier, wherein the enzyme includes at least one of Aspergillus niger acid β-mannanase, cellulase, a product enzyme of Bacillus noubii anaerobicus, and a neutral protease.

4. The hair repair composition as described in claim 1, characterized in that, The preparation method of the Dendrobium officinale extract includes the following steps: Dendrobium officinale stem powder was dispersed in water to obtain a solution, which was then heated at 60-80°C for water extraction. After water extraction, the immobilized enzyme was added to enzymatically hydrolyze the Dendrobium officinale to obtain the Dendrobium officinale extract.

5. The hair repair composition as described in claim 4, characterized in that, The mass ratio of the Dendrobium officinale stem powder to water is 1:(20-40).

6. The hair repair composition as described in claim 5, characterized in that, The amount of the immobilized enzyme added is 3-8% of the mass of the Dendrobium officinale stem powder.

7. The hair repair composition as claimed in claim 1, characterized in that, The tremella extract was prepared with a bacterial concentration of 10. 7 -10 9 The *C. utilis* seed culture was fermented and extracted from *Tremella fuciformis* to obtain the *Tremella fuciformis* seed culture. The mass ratio of the *C. utilis* seed culture to *Tremella fuciformis* was (2-5):(0.5-1.8).

8. A method for preparing the hair repair composition according to any one of claims 1 to 7, comprising the following steps: A hair repair composition can be obtained by mixing Dendrobium officinale extract, Tremella fuciformis extract, galactosomal yeast fermentation product filtrate, and Bifida ferment lysate fermentation product filtrate.

9. A hair care product, characterized in that, Includes the hair repair composition according to any one of claims 1 to 7.

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