A nano foliar spraying agent for improving high temperature resistance of wheat and a preparation method and application thereof

Abstract

The application belongs to the technical field of agricultural production, and particularly relates to a nano foliar spraying agent for improving the high-temperature resistance of wheat, and a preparation method and application thereof. The main component of the nano foliar spraying agent is a wolfberry-glycyrrhiza-based carbon quantum dot MIX. The application synthesizes a nano foliar spraying agent through one-step hydrothermal synthesis by using wolfberry as a raw material, and the nano foliar spraying agent can be used after simple purification. The material used in the application is ordinary wolfberry, which is cheap and easy to obtain. The synthesis method is simple, and the equipment used is common. The nano foliar spraying agent obtained by the method can effectively improve the high-temperature resistance of wheat.

Description

Technical Field

[0001] This invention belongs to the field of agricultural production technology, specifically relating to a nano-foliar spray that improves the high-temperature resistance of wheat, its preparation method, and its application. Background Technology

[0002] Global warming has become a major challenge to global agricultural production, particularly in Xinjiang, where extreme heat poses a severe obstacle to local agricultural development. As a crucial food crop widely cultivated in the region, the growth of wheat is of paramount importance. During wheat cultivation, high temperatures slow down photosynthesis, leading to a reduction in photosynthetic products. More seriously, high temperatures during the grain-filling stage directly hinder grain development, severely impacting wheat yield. This not only reduces the economic benefits of agricultural production but also threatens food security.

[0003] Plant nanobiology, as an emerging interdisciplinary field, offers unique insights into enhancing crop heat tolerance. While various nanomaterials synthesized from industrially sourced raw materials have been developed to improve photosynthetic efficiency and heat resistance in crops at high temperatures, their preparation often involves rigorous purification and safety assessment processes. Therefore, utilizing traditional Chinese medicinal plants as raw materials to synthesize nanomaterials that can improve wheat's heat tolerance is of great significance for the development of sustainable agriculture.

[0004] Therefore, exploring and applying an environmentally friendly, simple, and efficient raw material to improve the heat resistance of wheat is the most important issue at present. Summary of the Invention

[0005] The purpose of this invention is to provide a nano-foliar spray agent for improving the heat resistance of wheat, its preparation method, and its application. The raw materials for the nano-foliar spray agent provided by this invention are not only derived from traditional Chinese medicinal plants, but also reduce reliance on traditional industrial raw materials. Furthermore, by preparing it into a nano-foliar spray agent, it significantly improves the heat resistance of wheat.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] On the one hand, the present invention provides a nano-foliar spray to improve the high-temperature resistance of wheat, wherein the nano-foliar spray is composed of wolfberry-licorice-based carbon quantum dot MIX.

[0008] On the other hand, the present invention also provides a method for preparing a nano-foliar spray agent, comprising the following steps:

[0009] The wolfberry and licorice were pulverized and placed in a solvent and stirred to obtain a mixture. This mixture was then placed in a reaction vessel and heated at 160-170℃. After heating, it was cooled to room temperature. The reaction product was then centrifuged at 3000-5000 rpm, and the supernatant was collected to obtain wolfberry-licorice-based carbon quantum dot MIX, which was stored at 3-5℃ for later use.

[0010] Preferably, the nano-foliar spray contains a surfactant, wherein the surfactant is Silwet L-77, and accounts for 0.05% of the volume of the nanomaterial solution.

[0011] Preferably, the nano-foliar spray agent is a nanomaterial solution with a concentration of 80-300 mg / L.

[0012] Preferably, the mass ratio of wolfberry to licorice is (1-3):1.

[0013] On the other hand, the present invention also provides an application of a nano-foliar spray in improving the heat resistance of wheat.

[0014] Preferably, the nano-foliar spray agent is applied to the leaf surface using a pipette when the wheat has reached the stage of one leaf and one heart development.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0016] This invention utilizes wolfberry as a raw material to synthesize a nano-foliar spray agent via a one-step hydrothermal synthesis method. After simple purification, it is ready for use. The material used in this invention is common wolfberry, which is inexpensive and readily available; the synthesis method is simple, and the equipment used is common; the synthesized nanomaterials can be used after simple centrifugation; the nanomaterial solution obtained by this method can effectively improve the high-temperature resistance of wheat. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 TEM image for Control and MIX.

[0019] Figure 2 The aboveground fresh weight of wheat after 3 days of high temperature stress.

[0020] Figure 3 The fresh weight of the underground part of wheat after 3 days of high temperature stress.

[0021] Figure 4 The SPAD value of wheat under three consecutive days of high-temperature stress.

[0022] The Control group solution was deionized water containing 0.05% Silwet L-77; the MIX group solution was a 150 mg / L mixed solution of nanomaterials containing 0.05% Silwet L-77. Detailed Implementation

[0023] The purpose of this invention is to provide a nano-foliar spray that improves the heat resistance of wheat, its preparation method, and its application.

[0024] On one hand, the present invention provides a nano-foliar spray for improving the heat resistance of wheat, wherein the nano-foliar spray is a nanomaterial solution with a concentration of 80-300 mg / L, preferably 150 mg / L.

[0025] On the other hand, the present invention provides a method for preparing a nano-foliar spray agent, comprising the following steps: crushing wolfberry and licorice and placing them in a solvent and stirring to obtain a mixture, then placing this mixture in a reaction vessel and heating it at 160-170°C, cooling it to room temperature after heating, then centrifuging the reaction product at 3000-5000 rpm, taking the supernatant to obtain wolfberry-licorice-based carbon quantum dot MIX, and storing it at 3-5°C for later use.

[0026] The mass ratio of wolfberry to licorice is (1-3):1, preferably 1:1; the mixture is heated at 160-170℃; the reaction product is centrifuged at 3000-5000rpm. Preferably, the heating temperature is 160℃ and the centrifugation is 3000rpm.

[0027] On the other hand, the present invention provides an application of a nano-foliar spray in improving the heat resistance of wheat.

[0028] To further illustrate the present invention, the technical solutions provided by the present invention will be described in detail below with reference to the accompanying drawings and embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0029] Unless otherwise specified, the production processes, experimental methods, or testing methods involved in the embodiments of this invention are all conventional methods in the prior art, and their names and / or abbreviations are all conventional names in the field, which are very clear and distinct in the relevant application areas. Those skilled in the art can understand the conventional process steps based on the names and apply the corresponding equipment, and implement them according to conventional conditions or the conditions recommended by the manufacturer.

[0030] The various instruments, equipment, raw materials or reagents used in the embodiments of this invention are not subject to any special restrictions on their source. They are all conventional products that can be purchased through regular commercial channels and can be prepared according to conventional methods known to those skilled in the art.

[0031] Example 1

[0032] Crush goji berries and licorice root, weigh out 200 mg of goji berry powder and 200 mg of licorice root powder, mix well, add 20 mL of distilled water, and place in a 50 mL polytetrafluoroethylene (PTFE) reactor. The reaction temperature is 160 °C, and the reaction time is 6 hours. After cooling, aliquot the mixture into 1.5 mL centrifuge tubes, centrifuge at 3000 rpm for 1 hour, and collect the supernatant to obtain a brown liquid with a sweet aroma, which is the goji berry-licorice root-based carbon quantum dot (MIX) LWCMs. The LWCMs are finally stored at 4 °C for later use.

[0033] Example 2

[0034] Example 2 is basically the same as Example 1, except that the mass of each of the wolfberry and licorice powder is 300 mg. The heating temperature is 170°C and the centrifugation speed is 5000 rpm.

[0035] Example 3

[0036] Example 3 is basically the same as Example 1, except that the mass of each of the wolfberry and licorice powder is 500 mg. The heating temperature is 160℃ and the centrifugation speed is 3000 rpm.

[0037] Example 4

[0038] Based on the LWCMs prepared in Example 1, the following operations were performed: Select plump, uniformly sized, and undamaged wheat seeds, disinfect their surface with 75% medical alcohol for 30 seconds, then rinse 4-6 times with deionized water. Allow the disinfected seeds to air dry at room temperature until ready for use. Sow 5 seeds into each seed germination bag with 15 mL of distilled water.

[0039] Example 5

[0040] Treatment was initiated when the wheat reached the one-leaf-one-heart stage. Two different treatment solutions were prepared: one was deionized water containing 0.05% Silwet L-77 as a control; the other was a 150 mg / L nanomaterial mixture containing 0.05% Silwet L-77 for the experiment. The tip of a 1000 μl pipette was then shortened by 0.3 cm to avoid mechanical damage to the leaves from the sharp tip, and the solution was evenly applied to the leaf surface using a 1 mL pipette. After application, excess solution was blotted off with a paper towel. The treated wheat and the control wheat were placed in a low-light environment for 3 hours to acclimatize before being placed in a 39°C incubator for high-temperature stress.

[0041] Example 6

[0042] To evaluate the effect of spraying a mixed solution containing 0.05% Silwet L-77 surfactant and 150 mg / L nanomaterials on the chlorophyll content of wheat seedlings under three consecutive days of high-temperature stress, the SPAD values ​​of wheat seedlings treated with the mixed solution of 150 mg / L nanomaterials containing 0.05% Silwet L-77 and deionized water containing 0.05% Silwet L-77 were measured on days 1, 2, and 3 after high-temperature stress. The SPAD value of the first true leaf was measured five times for each leaf, and the average value was taken. Figure 4 ).

[0043] Example 7

[0044] Three days after stress, the seedlings were removed, photographed, and weighed. Wheat seedlings treated with the nanomaterial solution were significantly larger than those treated with 0.05% Silwet L-77. Figure 1 As shown, and with a higher above-ground portion as Figure 2 As shown and the fresh weight of the underground part Figure 3 As shown.

[0045] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Application of a nano-leaf surface spraying agent in improving the high temperature resistance of wheat; The composition of the nano-leaf surface spraying agent is wolfberry-glycyrrhiza-based carbon quantum dots MIX; The preparation method of the nano-leaf surface spraying agent comprises the following steps: Wolfberry and glycyrrhiza are crushed and placed in a solvent for stirring to obtain a mixture, then the mixture is placed in a reaction kettle for heating treatment at 160-170°C, after the heating is completed, it is cooled to room temperature, then the reaction product is subjected to centrifugal treatment at 3000-5000 rpm, the supernatant is taken, and wolfberry-glycyrrhiza-based carbon quantum dots MIX is obtained, which is stored at 3-5°C for standby use.

2. Use according to claim 1, characterized in that, The nano-leaf surface spraying agent is sprayed when the wheat grows to the one-leaf-one-heart development period, and is applied to the leaf surface by using a pipette gun.

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