Processing method and application of hair beautifying fish oil with high palatability for pet dogs and cats

Abstract

The invention relates to the technical field of fish oil processing for pet food. The processing method comprises the following steps: adding evening primrose oil and borage seed oil into fish oil, carrying out instantaneous high-temperature heating, cooling, adding a rosemary extract, uniformly mixing, adding a filter aid, stirring, winterizing, and filtering to obtain the fish oil with low fishy odor. The fish oil prepared by the method disclosed by the invention can effectively reduce the perception of people on fishy smell and peculiar smell of the fish oil, improve the intake willingness of dogs and cats on the fish oil, effectively improve the luster and smoothness of hairs of the dogs and cats, and play a role in beautifying hairs.

Description

Technical Field

[0001] The invention relates to the technical field of fish oil processing for pet food, and in particular to a fish oil processing method for reducing fishy odor and improving the palatability of pet dogs and cats, and an application of the fish oil in beautifying the fur of dogs and cats. Background Art

[0002] Pet hair-beautifying fish oil usually uses oil extracted from deep-sea fish as the main ingredient, and produces fish oil that has the effects of improving the hair quality, gloss, smoothness and reducing hair loss of pet dogs and cats. Pet hair-beautifying fish oil is rich in ω-3 unsaturated fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), the total content of which is usually greater than 3%. It is directly licked by pet dogs and cats or mixed into dog food or cat food to feed pet dogs and cats. However, the current fish oil raw materials contain organic amines such as dimethylamine and trimethylamine produced by the decomposition of trimethylamine oxide, so they have the disadvantages of strong fishy smell, obvious odor and strong irritation, which affects consumers' willingness to purchase fish oil products. At the same time, the fishy smell of fish oil will reduce the willingness and intake rate of dogs and cats who are highly sensitive to odors to eat fish oil and fish oil products.

[0003] It has been reported that the odor of the environment or fish processing products can be reduced by adding related components of evening primrose or rosemary. For example, rosemary essential oil is added to the deodorant spray to reduce the odor of odorous substances in the environment (a preparation method and application of a natural plant composite bio-enzyme deodorant spray, CN202310407358.5), and evening primrose water extract is used to marinate silver carp to reduce the fishy smell (a heart-strengthening and blood-activating aloe vera fish fillet and its preparation method, CN201510793154.5). The components and functional substances of extracts from different parts of plants vary greatly. Evening primrose seed oil and borage seed oil are functional oils extracted from evening primrose and borage seeds, similar to rosemary stem and leaf alcohol extracts, with a light fragrant grass smell. Although rosemary essential oil and evening primrose water extract have been reported to reduce the odor of some substances, there have been no reports on adding evening primrose seed oil, borage seed oil and rosemary stem and leaf alcohol extracts to fish oil processing to reduce people's perception of the fishy odor of fish oil and improve the palatability and willingness to eat fish oil in dogs and cats. Summary of the invention

[0004] The invention aims to provide a fish oil processing method which can reduce fishy smell and improve the palatability of pet dogs and cats, and the application of the fish oil in beautifying the fur of dogs and cats.

[0005] In a first aspect of the present invention, there is provided a method for processing fish oil for reducing fishy odor and improving palatability for dogs and cats, which is carried out according to the following steps:

[0006] Take evening primrose seed oil and borage seed oil and add them to salmon oil at 0.5% to 2.5% (volume ratio), respectively, and stir and mix for 0.5 to 1 hour. Introduce the mixed fish oil into a heating tube for instantaneous high-temperature heating, control the flow rate so that the time for the mixed fish oil to flow through the heating section is 10 to 20 seconds, and the heating temperature is 90 to 95°C. After the mixed fish oil is cooled, add rosemary extract at 0.5 to 1g / L, and stir and mix for 0.5 to 1h. Add activated white clay, stir and mix again, let it stand at a low temperature of 4 to 10°C for winterization, and then filter to obtain fish oil with low fishy odor.

[0007] The preparation method of the rosemary extract comprises the following steps: extracting the dried rosemary stem and leaf product twice with 60% to 80% ethanol, combining the extracts, concentrating under reduced pressure, and freeze-drying the extracts to obtain the rosemary extract.

[0008] The second aspect of the present invention provides the use of the fish oil prepared by the above method in beautifying the fur of pet dogs and cats.

[0009] Furthermore, the pets include but are not limited to dogs and cats, and can also be applied to other pets suitable for eating fish oil, such as foxes and chinchillas.

[0010] Furthermore, the hair beautifying effect includes but is not limited to improving the gloss and smoothness of hair.

[0011] Furthermore, the application method is to let the fish oil be licked directly by dogs and cats or to mix it into the pet staple food (or snacks) and feed it to pet dogs and cats.

[0012] The present invention has the following beneficial effects:

[0013] (1) The fishy smell mainly comes from the dimethylamine and trimethylamine produced after the decomposition of trimethylamine oxide in fish oil. The aldehyde substances (such as heptanal, benzoinaldehyde, etc.) contained in the borage seed oil and evening primrose seed oil used in the present invention can react with dimethylamine by nucleophilic addition, thereby reducing the content of dimethylamine and reducing the fishy smell. Rosemary extract contains a large amount of phenolic acid substances (such as rosmarinic acid, carnosic acid, rosmarinic phenol, etc.), which can neutralize the trimethylamine in fish oil and reduce the fishy smell. Therefore, when borage seed oil, evening primrose seed oil and rosemary extract are used in combination, they can effectively react with dimethylamine in fish oil and neutralize trimethylamine at the same time. High-temperature instantaneous heating helps to accelerate the nucleophilic addition reaction of aldehydes and dimethylamine in borage seed oil and evening primrose seed oil, and accelerate the neutralization reaction of phenolic acid substances and trimethylamine in rosemary extract, thereby improving the efficiency of removing dimethylamine and trimethylamine in fish oil. In addition, the winterization process combined with the addition of activated clay as a filter aid can further absorb the fishy odor in the fish oil. The fish oil prepared by the process of the present invention can effectively reduce people's perception of the fishy odor of the fish oil.

[0014] (2) In the present invention, instantaneous heating is adopted, which can effectively control the processing time and reduce the damage of high temperature to unsaturated fatty acids EPA and DHA. After winterization, saturated fatty acids in fish oil are crystallized at low temperature, which are filtered and removed after plate and frame filter pressing. The relative content of unsaturated fatty acids such as EPA and DHA in fish oil is improved. After winterization, the content of EPA and DHA in fish oil is increased by more than 4.5%. At the same time, borage seed oil and evening primrose seed oil are rich in γ-linolenic acid, have good antioxidant and anti-inflammatory effects, can not only promote the growth of dog and cat hair, but also slow down the hair loss and depilation problem caused by oxidative stress in dogs and cats, and play a role in strengthening and beautifying hair.

[0015] The fish oil processed by the method of the present invention has low fishy odor, and the fishy odor sensory score of the fish oil by humans is only 1.42-1.67 points, which is significantly reduced by 43.39%-67.43% compared with the control; the palatability score and willingness to eat rate of the fish oil by cats reach 4.57-4.67 points and 90%-95% respectively, which are 1.25-1.95 times and 1.38-9.50 times of the control respectively; the palatability score and willingness to eat rate of the fish oil by dogs reach 4.78-4.89 points and 95%-100% respectively, which are 1.19-1.59 times and 1.19-2.22 times of the control respectively. At the same time, the fish oil has the effect of improving the gloss and smoothness of dog and cat hair. The gloss score and smoothness score of cat hair reached 4.37-4.61 points and 4.39-4.55 points, respectively, which were 1.14-1.73 times and 1.17-1.64 times that of the control; the gloss score and smoothness score of dog hair reached 4.29-4.53 points and 4.19-4.48 points, respectively, which were 1.09-1.77 times and 1.10-1.58 times that of the control. DETAILED DESCRIPTION

[0016] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those of ordinary skill in the art. The present invention will be further described in detail below in conjunction with specific embodiments and with reference to data. It should be understood that these embodiments are only further explanations of the present invention and cannot be construed as limiting the scope of protection of the present invention. Technical engineers in this field may make some non-essential improvements and adjustments to the present invention based on the content of the above invention.

[0017] In the following examples, various processes and methods not described in detail are conventional methods known in the art. The sources, trade names and components of the reagents used are indicated when they first appear, and the same reagents used thereafter are the same as those indicated for the first time unless otherwise specified.

[0018] The salmon oil, borage seed oil, evening primrose seed oil and dried rosemary stem and leaf products used in the present embodiment and the comparative example are all commercially available.

[0019] The sensory evaluation method of fishy odor in this embodiment and control example is as follows:

[0020] 20mL of fish oil sample was placed in a centrifuge tube, and the tester smelled the sample at 5cm from the tube mouth for sensory evaluation. The higher the degree of fishy odor, the higher the score. The scoring method is: 1 point, no obvious fishy odor; 2 points, slight fishy odor, acceptable; 3 points, obvious fishy odor, tolerable; 4 points, heavy fishy odor, unbearable; 5 points, strong fishy odor, unbearable.

[0021] The palatability evaluation method for dogs and cats in this embodiment and control example is as follows:

[0022] Twenty healthy dogs and cats that were eating normally and not in estrus were arranged to participate in the test. 5 mL of fish oil sample was added to the test bowl and placed in front of the dogs and cats that had been fasting for more than 10 hours. After guiding the dogs and cats and making sure they paid attention to the fish oil sample, the reactions of the dogs and cats to the fish oil were observed within 10 minutes, and the palatability score and willingness to eat rate were calculated.

[0023] Palatability scoring method: 1 point, obvious retreat after sniffing, no food intake, and retching; 2 points, no food intake after sniffing, but turning around or burying the food; 3 points, licking the nose after sniffing, no food intake or only one food intake; 4 points, willingness to eat, eating 2 or more times; 5 points, active eating multiple times, and eating more than half of the food intake.

[0024] Willingness to eat rate = number of dogs (cats) willing to eat (palatability score ≥ 4 points) / total number of dogs (cats) participating in the test.

[0025] The glossiness scores of dog and cat hair in this embodiment and control example are as follows:

[0026] After establishing familiarity with the dog or cat, the hair gloss of the head, back, sides, and tail is scored under the same light source, brightness, angle, and distance. The scoring method is: 1 point, dull and lusterless; 2 points, a small amount of glossy reflection; 3 points, some glossy reflection; 4 points, obvious reflection, soft gloss; 5 points, obvious reflection, bright gloss. The average of the gloss scores of the hair in each part is taken as the hair gloss score of the dog or cat.

[0027] The smoothness scores of dog and cat hair in this embodiment and control example are as follows:

[0028] After establishing familiarity with the dog or cat, the tester washes his hands, disinfects them and dries them. He then presses the hair on the head, back, sides and tail, touches it and scores the smoothness respectively. The scoring method is as follows: 1 point, rough touch, reverse hair, pricking and stinging; 2 points, rough touch, a small amount of reverse hair, a small amount of pricking without stinging; 3 points, the hair is not rough, there is no obvious reverse hair, and it is not stinging; 4 points, the hair is relatively smooth, there is no reverse hair, it is not stinging, and there are a few knots when combing with a flat ruler comb; 5 points, the hair is very smooth, there is no reverse hair, it is not stinging, and there are no knots when combing with a flat ruler comb. The average of the hair smoothness scores of each part is taken as the hair smoothness score of the dog or cat.

[0029] In the present embodiment and control examples, the dog and cat feeding test animals are grouped and tested as follows:

[0030] On the first day of the dog and cat feeding test, palatability scores and willingness to eat tests were performed, and dog and cat hair gloss scores and smoothness scores were performed after 14 days of feeding. Each fish oil sample prepared in the control example and the embodiment was added to the dog and cat diet for supplementary feeding at 2% (mass ratio), and the initial hair gloss scores and hair smoothness scores of the dogs and cats participating in the test were less than 2 points. In the cat feeding test, 16 Abyssinian, Ragdoll, American Shorthair, British Shorthair and Maine Coon cats were selected and evenly divided into 8 groups according to body weight, with 10 cats in each group (each group containing 2 cats of the above 5 types). In the dog feeding test, 16 Pomeranians, Chihuahuas, Teddy, Schnauzers and Bichon Frise dogs were selected and evenly divided into 8 groups according to body weight, with 10 cats in each group (each group containing 2 dogs of the above 5 types).

[0031] Comparative Example 1

[0032] Unprocessed salmon oil was subjected to sensory evaluation for fishy odor in humans and palatability test in dogs and cats. The results showed that humans gave the fish oil a sensory score of 4.31 for fishy odor; the cat palatability score was 2.43, with a willingness to consume rate of only 10%; the dog palatability score was 3.08, with a willingness to consume rate of 45%.

[0033] After 14 days of continuous supplementation, the cat's fur gloss score was 2.73 points, and the smoothness score was 2.86 points; the dog's fur gloss score was 2.56 points, and the smoothness score was 2.83 points.

[0034] Comparative Example 2

[0035] Evening primrose seed oil and borage seed oil were added to salmon oil at 2.5% (volume ratio) respectively, and stirred for 1 hour. Rosemary extract was then added at 1 g / L, and stirred for another 1 hour to obtain a fish oil sample.

[0036] The preparation method of the rosemary extract comprises the following steps: crushing a dried product of rosemary stems and leaves, dissolving 100 g of the powder in 1000 mL of an ethanol solution (with anhydrous ethanol having a volume fraction of 60%), stirring and extracting at 50° C. for 1 h, filtering and retaining the filtrate, extracting the residue again under the same conditions, combining the two extraction filtrates, concentrating under reduced pressure, and freeze-drying the mixture, grinding the powder and passing it through an 80-mesh sieve to obtain the rosemary extract.

[0037] Fish oil samples were taken for human sensory evaluation of the fishy and odor and for palatability tests on dogs and cats. The results showed that humans gave the fishy and odor sensory evaluation of 4.22 points; the cat palatability score was 2.83 points, with a willingness to eat rate of only 20%; the dog palatability score was 3.44 points, with a willingness to eat rate of 60%.

[0038] After 14 days of continuous supplementation, the cat's hair gloss score was 3.15 points, and the smoothness score was 3.24 points; the dog's hair gloss score was 2.82 points, and the smoothness score was 2.99 points.

[0039] Comparative Example 3

[0040] The salmon oil was introduced into the heating tube for instantaneous heating, and the flow rate was controlled so that the mixed fish oil flowed through the heating section for 10 seconds, and the heating temperature was 95°C. After the fish oil was cooled, activated white clay was added at a rate of 50 g / L, stirred and mixed for 10 minutes, and then left to stand at 4°C for 24 hours for winterization. The fish oil was filtered through a plate and frame filter press to obtain a fish oil sample.

[0041] Fish oil samples were taken for human sensory evaluation of the fishy and odor and for palatability tests on dogs and cats. The results showed that humans gave the fishy and odor sensory evaluation of 4.36 points; cats gave the palatability evaluation of 2.39 points, with a willingness to consume rate of only 10%; and dogs gave the palatability evaluation of 3.21 points, with a willingness to consume rate of 55%.

[0042] After 14 days of continuous supplementation, the cat's fur gloss score was 2.67 points, and the smoothness score was 2.78 points; the dog's fur gloss score was 2.73 points, and the smoothness score was 3.08 points.

[0043] Comparative Example 4

[0044] Take evening primrose seed oil and add it to salmon oil at 5% (volume ratio), and stir and mix for 1 hour. Introduce the mixed fish oil into a heating tube for instantaneous heating, control the flow rate so that the mixed fish oil flows through the heating section for 10 seconds, and the heating temperature is 95°C. After the mixed fish oil is cooled, add activated white clay at 50g / L, stir and mix for 10 minutes, stand at 4°C for 24 hours for winterization, and filter the fish oil through a plate and frame filter press to obtain a fish oil sample.

[0045] Fish oil samples were taken for human fishy odor evaluation and dog and cat palatability test. The results showed that humans gave the fishy odor sensory score of 2.95 points; cat palatability score was 3.48 points, with a willingness to consume rate of 55%; dog palatability score was 3.97 points, with a willingness to consume rate of 75%.

[0046] After 14 days of continuous supplementation, the cat's hair gloss score was 3.51 points, and the smoothness score was 3.65 points; the dog's hair gloss score was 3.44 points, and the smoothness score was 3.62 points.

[0047] Comparative Example 5

[0048] Borage seed oil was added to salmon oil at 5% (volume ratio) and stirred for 1 hour. The mixed fish oil was introduced into a heating tube for instantaneous heating, and the flow rate was controlled so that the mixed fish oil flowed through the heating section for 10 seconds at a heating temperature of 95°C. After the mixed fish oil was cooled, activated white clay was added at 50 g / L, stirred for 10 minutes, and then winterized at 4°C for 24 hours. The fish oil was filtered through a plate and frame filter press to obtain a fish oil sample.

[0049] Fish oil samples were taken for human sensory evaluation of the fishy and odor and for palatability tests on dogs and cats. The results showed that humans gave the fishy and odor sensory score of 3.24 points; cats' palatability score was 3.67 points, with a willingness to consume rate of 65%; and dogs' palatability score was 4.03 points, with a willingness to consume rate of 80%.

[0050] After 14 days of continuous supplementation, the cat's hair gloss score was 3.35 points, and the smoothness score was 3.52 points; the dog's hair gloss score was 3.72 points, and the smoothness score was 3.54 points.

[0051] Comparative Example 6

[0052] The salmon oil was introduced into the heating tube for instantaneous heating, and the flow rate was controlled so that the mixed fish oil flowed through the heating section for 10 seconds at a heating temperature of 95°C. After the fish oil was cooled, rosemary extract was added at 1 g / L and stirred for 1 hour. Activated white clay was added at 50 g / L, and stirred and mixed again for 10 minutes, and then left to stand at 4°C for 24 hours for winterization treatment. The fish oil was filtered through a plate and frame filter press to obtain a fish oil sample.

[0053] The preparation method of the rosemary extract comprises the following steps: crushing a dried product of rosemary stems and leaves, dissolving 100 g of the powder in 1000 mL of an ethanol solution (with anhydrous ethanol having a volume fraction of 60%), stirring and extracting at 50° C. for 1 h, filtering and retaining the filtrate, extracting the residue again under the same conditions, combining the two extraction filtrates, concentrating under reduced pressure, and freeze-drying the mixture, grinding the powder and passing it through an 80-mesh sieve to obtain the rosemary extract.

[0054] Fish oil samples were taken for human sensory evaluation of the fishy and odor and for palatability tests on dogs and cats. The results showed that humans gave the fishy and odor sensory score of 3.45 points; cats' palatability score was 3.34 points, with a willingness to eat rate of 60%; and dogs' palatability score was 3.82 points, with a willingness to eat rate of 75%.

[0055] After 14 days of continuous supplementation, the cat's fur gloss score was 3.83 points, and the smoothness score was 3.74 points; the dog's fur gloss score was 3.95 points, and the smoothness score was 3.81 points.

[0056] Example 1

[0057] Take evening primrose seed oil and borage seed oil and add them to salmon oil at 2.5% (volume ratio) respectively, and stir and mix for 1 hour. Introduce the mixed fish oil into the heating tube for instantaneous heating, control the flow rate so that the mixed fish oil flows through the heating section for 10 seconds, and the heating temperature is 95°C. After the mixed fish oil is cooled, add rosemary extract at 1g / L and stir and mix for 1h. Add activated white clay at 50g / L, stir and mix again for 10min, stand at 4°C for 24h for winterization, filter the fish oil through a plate and frame filter press, and obtain fish oil with low fishy odor.

[0058] The preparation method of the rosemary extract comprises the following steps: crushing a dried product of rosemary stems and leaves, dissolving 100 g of the powder in 1000 mL of an ethanol solution (with anhydrous ethanol having a volume fraction of 60%), stirring and extracting at 50° C. for 1 h, filtering and retaining the filtrate, extracting the residue again under the same conditions, combining the two extraction filtrates, concentrating under reduced pressure, and freeze-drying the mixture, grinding the powder and passing it through an 80-mesh sieve to obtain the rosemary extract.

[0059] Fish oil samples were taken for human sensory evaluation of the fishy and odor and for palatability tests on dogs and cats. The results showed that the human sensory score for the fishy and odor of the fish oil was 1.67 points; the cat palatability score was 4.57 points, and the willingness to eat rate was 90%; the dog palatability score was 4.89 points, and the willingness to eat rate was 100%.

[0060] After 14 days of continuous supplementation, the cat's fur gloss score was 4.37 points, and the smoothness score was 4.46 points; the dog's fur gloss score was 4.41 points, and the smoothness score was 4.148 points.

[0061] Example 2

[0062] Take evening primrose seed oil and borage seed oil and add them to salmon oil at 0.5% (volume ratio) respectively, and stir and mix for 0.5h. Introduce the mixed fish oil into a heating tube for instantaneous heating, control the flow rate so that the mixed fish oil flows through the heating section for 20s, and the heating temperature is 90°C. After the mixed fish oil is cooled, add rosemary extract at 0.5g / L and stir and mix for 0.5h. Add activated white clay at 50g / L, stir and mix again for 10min, let it stand at 4°C for 24h for winterization, and filter the fish oil through a plate and frame filter press to obtain fish oil with low fishy odor.

[0063] The preparation method of the rosemary extract comprises the following steps: crushing a dried product of rosemary stems and leaves, dissolving 100 g of the powder in 1000 mL of an ethanol solution (with an anhydrous ethanol volume fraction of 80%), stirring and extracting at 50° C. for 1 h, filtering and retaining the filtrate, extracting the residue again under the same conditions, combining the two extraction filtrates, concentrating under reduced pressure, and freeze-drying the mixture, grinding the powder and passing it through an 80-mesh sieve to obtain the rosemary extract.

[0064] Fish oil samples were taken for human sensory evaluation of the fishy and odor and for palatability tests on dogs and cats. The results showed that the human sensory score for the fishy and odor of the fish oil was 1.48 points; the cat palatability score was 4.67 points, and the willingness to eat rate was 95%; the dog palatability score was 4.85 points, and the willingness to eat rate was 95%.

[0065] After 14 days of continuous supplementation, the cat's fur gloss score was 4.52 points, and the smoothness score was 4.55 points; the dog's fur gloss score was 4.29 points, and the smoothness score was 4.33 points.

[0066] Example 3

[0067] Take evening primrose seed oil and borage seed oil and add them to salmon oil at 1.5% (volume ratio) respectively, and stir and mix for 0.75h. Introduce the mixed fish oil into a heating tube for instantaneous heating, control the flow rate so that the mixed fish oil flows through the heating section for 15s, and the heating temperature is 92.5°C. After the mixed fish oil is cooled, add rosemary extract at 0.75g / L and stir and mix for 0.75h. Add activated white clay at 50g / L, stir and mix again for 10min, let it stand at 7°C for 24h for winterization, and filter the fish oil through a plate and frame filter press to obtain fish oil with low fishy odor.

[0068] The preparation method of the rosemary extract comprises the following steps: crushing a dried product of rosemary stems and leaves, dissolving 100 g of the powder in 1000 mL of an ethanol solution (with anhydrous ethanol having a volume fraction of 70%), stirring and extracting at 50° C. for 1 h, filtering and retaining the filtrate, extracting the residue again under the same conditions, combining the two extraction filtrates, concentrating under reduced pressure, and freeze-drying the mixture, grinding the powder and passing it through an 80-mesh sieve to obtain the rosemary extract.

[0069] Fish oil samples were taken for human sensory evaluation of the fishy and odor and for palatability tests on dogs and cats. The results showed that the human sensory score for the fishy and odor of the fish oil was 1.42 points; the cat palatability score was 4.62 points, and the willingness to eat rate was 95%; the dog palatability score was 4.78 points, and the willingness to eat rate was 100%.

[0070] After 14 days of continuous supplementation, the cat's fur gloss score was 4.61 points, and the smoothness score was 4.39 points; the dog's fur gloss score was 4.53 points, and the smoothness score was 4.19 points.

Claims

1. A method for processing highly palatable pet dog and cat hair-beautifying fish oil, characterized in that Follow these steps: Take evening primrose oil and borage seed oil at 0.5% to 2.5% (volume ratio) and add them to salmon oil, stirring and mixing for 0.5 to 1 hour. Introduce the mixed fish oil into a heating tube for instantaneous high-temperature heating, and control the flow rate so that the mixed fish oil flows through the heating section for 10 to 20 seconds, and the heating temperature is 90 to 95°C. After the mixed fish oil is cooled, add rosemary extract at 0.5 to 1g / L, stirring and mixing for 0.5 to 1h. Add activated white clay, stir and mix again, and then winterize (stand at a low temperature of 4 to 10°C), and then filter to obtain fish oil with low fishy odor.

2. The rosemary extract according to claim 1, characterized in that The preparation method is prepared according to the following steps: using 60% to 80% ethanol to extract the dried rosemary stem and leaf product twice, combining the extracts to carry out reduced pressure concentration, and then freeze-drying to obtain the rosemary extract.

3. Use of the low-smell fish oil according to claim 1 in beautifying the fur of pet dogs and cats.