Eye care composition and eye care solution

Abstract

The invention discloses an eye care composition and an eye care solution, and belongs to the technical field of cosmetics, the eye care composition comprises hyaluronic acid substances, a Chinese herbaceous peony root extract and a calendula extract according to a mass ratio of 1: (0.5-8): (2-10); the sodium hyaluronate substances comprise a sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1: (0.25-4): (0.25-4): (0.5-1). The eye care solution containing the eye care composition has excellent moisturizing and repairing effects, can effectively solve the problems of eye dryness and eye acerbity, relieves eye fatigue, and comprehensively improves the eye condition.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an eye care composition and an eye care solution. Background Art

[0002] With the popularity of electronic devices in modern society, staring at screens for a long time has become a daily routine, leading to increasingly serious eye health problems such as eye fatigue, dryness, and decreased vision. Most of the eye care products on the market currently focus on a single ingredient or a simple combination, and have limited effects on alleviating complex eye problems. Therefore, it is urgent to develop an eye care solution that can comprehensively improve eye conditions and has multiple functions.

[0003] In view of this, this application is filed. Summary of the invention

[0004] The object of the present invention is to overcome the deficiencies of the prior art and provide an eye care composition and an eye care solution. The eye care solution comprising the eye care composition of the present invention has excellent moisturizing and repairing effects, can effectively improve the problem of dry eye skin, can effectively solve the problem of dry eyes and eye astringency, relieve eye fatigue, and comprehensively improve eye conditions.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] An eye care composition, comprising a hyaluronic acid substance, a peony root extract and a calendula extract in a mass ratio of 1: (0.5-8): (2-10);

[0007] The sodium hyaluronate-like substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:(0.25-4):(0.25-4):(0.5-1).

[0008] Peony root extract is rich in a variety of natural ingredients, such as flavonoids and polysaccharides, which help to enhance skin hydration and maintain skin moisture balance. It has excellent anti-inflammatory effects and can effectively relieve eye fatigue, redness, swelling, inflammation and other symptoms caused by allergies or environmental irritation. The antioxidant components of peony root extract can help the skin around the eyes resist free radical damage, reduce signs of aging, and protect the skin around the eyes from ultraviolet rays and environmental pollution. Peony root extract can relieve eye skin tightness and discomfort, promote eye skin comfort and repair, and is especially suitable for sensitive skin. At the same time, peony root extract can promote local blood circulation in the eyes and improve blood flow in the eyes.

[0009] The present invention creatively uses hyaluronic acid, peony root extract and calendula extract as eye care compositions, and produces synergistic effects in eye moisturizing and repairing through their unique mechanisms of action (such as moisturizing, anti-inflammatory, anti-oxidation and repair). It can provide immediate comfort and moisturizing effects, and can also repair and protect the eye skin for a long time, making it more moisturized and smooth, reducing eye problems such as fine lines, dark circles and eye bags, and improving the overall health of the eye skin.

[0010] As a preferred embodiment of the present invention, the hyaluronic acid substances, peony root extract and calendula extract are included in the mass ratio of 1: (2-5): (4-7), especially when the mass ratio of the three is controlled within this range, the compatibility is better, and a more ideal synergistic effect can be exerted, the moisturizing and repairing effect can be further improved, the problem of dry eye skin can be effectively improved, eye fatigue can be relieved, and the eye condition can be comprehensively improved.

[0011] As a preferred embodiment of the present invention, the sodium hyaluronate-based substance includes sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1: (0.25-2): (0.5-4.5): (0.25-2.5).

[0012] Sodium Hyaluronate: Sodium Hyaluronate can absorb and lock in moisture, enhance skin hydration, keep the skin soft and moisturized. By improving skin hydration, it helps reduce the appearance of fine lines and wrinkles, making the skin look fuller and smoother, while promoting skin repair, enhancing skin barrier function, and helping the skin return to a healthy state.

[0013] Sodium hyaluronate cross-linked polymer: It forms a three-dimensional network structure through cross-linking reaction, which can provide long-term moisturizing effect and slow down the loss of water. Its unique cross-linking structure and cross-linked sodium hyaluronate can form a thin film on the skin surface, improving the elasticity and firmness of the skin. It can fully fill the tiny texture defects of the skin and make the skin look smoother. It stays on the skin surface for a long time and has more excellent moisturizing effect and moisturizing stability.

[0014] Hydrolyzed Sodium Hyaluronate: Hydrolyzed Sodium Hyaluronate can penetrate into the skin layer more quickly, provide deep moisturizing effect, effectively improve skin hydration, reduce water evaporation, promote skin cell regeneration, and help repair damaged skin barrier.

[0015] Acetylated sodium hyaluronate: Acetylation treatment can improve the stability of sodium hyaluronate, prevent its degradation in the formula, and improve the stability of other ingredients. Acetylated sodium hyaluronate has excellent antioxidant effects, can slow down the aging process, and reduce the damage of free radicals to the skin. Its unique chemical structure makes it more effective in repairing damaged skin and protecting the skin barrier.

[0016] Among them, the hyaluronic acid substances composed of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate according to the above-mentioned specific ratio have excellent moisturizing and repairing effects. Sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate form a unique and stable four-dimensional hyaluronic acid network structure, which gives full play to their respective advantages, so that they can fully stay on the skin surface and penetrate into the deep layer of the skin more quickly, promote skin regeneration, prevent it from being oxidized, and provide comprehensive hydration for the skin. Hydrolyzed sodium hyaluronate penetrates into the deep layer of the skin to provide deep moisturizing, while cross-linked sodium hyaluronate forms a strong moisturizing barrier on the surface to reduce water loss. Acetylated sodium hyaluronate not only provides long-lasting moisturizing, but also has a certain antioxidant effect, which helps to repair the skin. The antioxidant effect provided by acetylated sodium hyaluronate combined with the moisturizing effect of sodium hyaluronate can slow down the skin aging process, reduce the formation of fine lines and wrinkles, and make the skin smoother and firmer; thereby exerting a more excellent moisturizing and repairing effect.

[0017] As a preferred embodiment of the present invention, the sodium hyaluronate-based substance includes sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:(0.5-1.5):(1.5-2.5):(1-2).

[0018] As a preferred embodiment of the present invention, the method for preparing the calendula extract comprises the following steps:

[0019] Adding marigold powder, cellulase and β-glucanase into water, performing enzymolysis and filtering;

[0020] The pH value of the filtrate is adjusted to 6.2-6.8, and the filtrate is passed through a macroporous adsorption resin at a flow rate of 1-1.5 BV / h, and the elution rate is 1-1.5 BV / h. First, 1-1.5 BV is washed with water, and then 1-2 BV is washed with a 75-85 wt% ethanol solution. The ethanol solution eluate is collected and dried to obtain a calendula extract.

[0021] The present invention creatively performs enzymatic hydrolysis on calendula powder and applies macroporous adsorption resin to obtain calendula extract with excellent moisturizing and repairing properties. The calendula extract can promote skin repair and regeneration, help improve the health of eye skin, reduce damage caused by ultraviolet rays, wind and sun exposure, etc., can effectively promote cell regeneration, and accelerate the repair process of damaged skin. The calendula extract can enhance the skin barrier function, reduce water loss, help keep the eye skin soft and moisturized, help improve the problems of dry eyes, fine lines, etc., keep the eye skin moist and smooth, and at the same time, the calendula extract helps prevent problems caused by eye skin infection and reduces inflammatory reactions caused by bacteria.

[0022] The marigold powder can be purchased from the market, or obtained by crushing marigold.

[0023] Wherein, the particle size of the marigold powder is 50 to 200 meshes.

[0024] As a preferred embodiment of the present invention, the mass ratio of the marigold pollen, cellulase, β-glucanase and water is 1: (0.02-0.04): (0.01-0.02): (2-20).

[0025] As a preferred embodiment of the present invention, the enzymolysis temperature is 48-55° C., and the enzymolysis time is 2-6 hours.

[0026] As a preferred embodiment of the present invention, the macroporous adsorption resin is at least one of AB-8 macroporous adsorption resin, D101 macroporous adsorption resin, and HPD826 macroporous adsorption resin.

[0027] The present invention also provides an eye care solution, comprising the following components in percentage by mass: 0.5-1.5% of an eye care composition, 0.001-0.005% of artificial bezoar, 0.005-0.02% of vitamin B substances, 0.01-0.06% of ginseng substances, 0.05-0.2% of menthone glycerol acetal, 0.001-0.3% of dipeptide-2, 0.1-0.4% of yeast polypeptides, 0.1-0.5% of safflower extract, 0.01-0.5% of lutein, 0.01-0.6% of wormwood oil, 0.2-0.8% of panthenol, 0.01-0.6% of tocopherol, 2-5% of polyols, 0.04-1% of Tween 20, and the balance of water;

[0028] The eye care composition is the eye care composition described above.

[0029] As a preferred embodiment of the present invention, the ginseng substance includes at least one of ginseng root water, ginseng extract, ginseng root extract, and hydrolyzed ginsenosides.

[0030] As a preferred embodiment of the present invention, the polyol includes at least one of glycerol, 1,2-propylene glycol, 1,3-propylene glycol, 1,3-butylene glycol, 1,4-butylene glycol, 1,2-pentanediol, and 1,5-pentanediol.

[0031] As a preferred embodiment of the present invention, the vitamin B substance includes at least one of biotin, folic acid, vitamin B12, vitamin B3, pantothenic acid, vitamin B6, vitamin B2, and vitamin B1.

[0032] The beneficial effect of the present invention is that the present invention uses hyaluronic acid substances, peony root extracts and calendula extracts as eye care compositions, and produces synergistic effects in eye moisturizing and repairing through their own unique mechanisms of action (such as moisturizing, anti-inflammatory, anti-oxidation and repair). It can provide immediate comfort and moisturizing effects, and can also repair and protect the eye skin for a long time, can effectively solve the problems of dry eyes and astringent eyes, relieve eye fatigue, comprehensively improve the eye condition, make it more moisturized and smooth, reduce eye problems such as fine lines, dark circles and eye bags, and improve the overall health of the eye skin. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application are described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

[0034] In the present application, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0035] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0036] In the present application, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0037] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0038] Unless otherwise specified, the components, raw materials or instruments used in the embodiments and comparative examples of the present invention are all commercially available raw materials or instruments, and the components and raw materials used in each parallel experiment are all of the same kind.

[0039] Wherein, the raw materials of the embodiments and comparative examples are from the following sources:

[0040] Peony root extract: sourced from Xi'an Qiancao Biotechnology Co., Ltd.

[0041] Hydrolyzed sodium hyaluronate: from Shandong Zhongshan Biotechnology Co., Ltd.

[0042] Acetylated sodium hyaluronate: from Shandong Zhongshan Biotechnology Co., Ltd.

[0043] Sodium hyaluronate: Sourced from Shandong Zhongshan Biotechnology Co., Ltd.

[0044] Sodium hyaluronate cross-linked polymer: from Shandong Liyang Biotechnology Co., Ltd.

[0045] Cellulase: Derived from McLean's reagent.

[0046] β-Glucanase: Derived from McLean's reagent.

[0047] AB-8 macroporous adsorption resin: from Langfang Miaoyang Chemical Co., Ltd.

[0048] Artificial bezoar: from Resentik Biotechnology.

[0049] Ginseng extract: from Langlin Biotechnology.

[0050] Dipeptide-2: Derived from polymer organisms.

[0051] Yeast polypeptides: from Jufeng Chemical.

[0052] Safflower extract: from Langlin Biotechnology.

[0053] Chuanxiong extract: from Langlin Biotechnology.

[0054] Salvia miltiorrhiza extract: from Langlin Biotechnology.

[0055] The remaining raw materials are of conventional cosmetic commercial grade.

[0056] The following examples are provided to facilitate understanding of the present invention. These examples are not provided to limit the scope of the claims.

[0057] Example 1

[0058] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0059] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:4:5.

[0060] The hyaluronic acid-based substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:0.5:1.5:1.

[0061] Wherein, the preparation method of the calendula extract comprises the following steps:

[0062] The marigolds are crushed into 100 meshes to obtain marigold powder, and the marigold powder, cellulase, and β-glucanase are added to water, and enzymolysis is performed at 50° C. for 4 hours, and filtering is performed; the mass ratio of the marigold powder, cellulase, β-glucanase, and water is 1:0.03:0.02:10;

[0063] The pH of the filtrate was adjusted to 6.6, and passed through AB-8 macroporous adsorption resin at a flow rate of 1 BV / h, the elution rate was 1 BV / h, 1.5 BV was first washed with water, and then 1.5 BV was washed with 80 wt% ethanol solution, the ethanol solution eluate was collected and dried to obtain a calendula extract.

[0064] Example 2

[0065] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0066] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:2:7.

[0067] The hyaluronic acid-based substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:0.5:1.5:1.

[0068] Wherein, the preparation method of the calendula extract comprises the following steps:

[0069] The marigolds are crushed into 100 meshes to obtain marigold powder, and the marigold powder, cellulase, and β-glucanase are added to water, and enzymolysis is performed at 50° C. for 4 hours, and filtering is performed; the mass ratio of the marigold powder, cellulase, β-glucanase, and water is 1:0.03:0.02:10;

[0070] The pH of the filtrate was adjusted to 6.6, and passed through AB-8 macroporous adsorption resin at a flow rate of 1 BV / h, the elution rate was 1 BV / h, 1.5 BV was first washed with water, and then 1.5 BV was washed with 80 wt% ethanol solution, the ethanol solution eluate was collected and dried to obtain a calendula extract.

[0071] Example 3

[0072] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0073] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:5:4.

[0074] The hyaluronic acid-based substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:0.5:1.5:1.

[0075] Wherein, the preparation method of the calendula extract comprises the following steps:

[0076] The marigolds are crushed into 100 meshes to obtain marigold powder, and the marigold powder, cellulase, and β-glucanase are added to water, and enzymolysis is performed at 50° C. for 4 hours, and filtering is performed; the mass ratio of the marigold powder, cellulase, β-glucanase, and water is 1:0.03:0.02:10;

[0077] The pH of the filtrate was adjusted to 6.6, and passed through AB-8 macroporous adsorption resin at a flow rate of 1 BV / h, the elution rate was 1 BV / h, 1.5 BV was first washed with water, and then 1.5 BV was washed with 80 wt% ethanol solution, the ethanol solution eluate was collected and dried to obtain a calendula extract.

[0078] Example 4

[0079] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0080] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:0.5:2.

[0081] The hyaluronic acid-based substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:0.5:1.5:1.

[0082] Wherein, the preparation method of the calendula extract comprises the following steps:

[0083] The marigolds are crushed into 100 meshes to obtain marigold powder, and the marigold powder, cellulase, and β-glucanase are added to water, and enzymolysis is performed at 50° C. for 4 hours, and filtering is performed; the mass ratio of the marigold powder, cellulase, β-glucanase, and water is 1:0.03:0.02:10;

[0084] The pH of the filtrate was adjusted to 6.6, and passed through AB-8 macroporous adsorption resin at a flow rate of 1 BV / h, the elution rate was 1 BV / h, 1.5 BV was first washed with water, and then 1.5 BV was washed with 80 wt% ethanol solution, the ethanol solution eluate was collected and dried to obtain a calendula extract.

[0085] Example 5

[0086] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0087] The eye care composition comprises hyaluronic acid substances, peony root extract and calendula officinalis extract in a mass ratio of 1:8:10.

[0088] The hyaluronic acid-based substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:0.5:1.5:1.

[0089] Wherein, the preparation method of the calendula extract comprises the following steps:

[0090] The marigolds are crushed into 100 meshes to obtain marigold powder, and the marigold powder, cellulase, and β-glucanase are added to water, and enzymolysis is performed at 50° C. for 4 hours, and filtering is performed; the mass ratio of the marigold powder, cellulase, β-glucanase, and water is 1:0.03:0.02:10;

[0091] The pH of the filtrate was adjusted to 6.6, and passed through AB-8 macroporous adsorption resin at a flow rate of 1 BV / h, the elution rate was 1 BV / h, 1.5 BV was first washed with water, and then 1.5 BV was washed with 80 wt% ethanol solution, the ethanol solution eluate was collected and dried to obtain a calendula extract.

[0092] Example 6

[0093] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0094] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:4:5.

[0095] The hyaluronic acid substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:1.5:2.5:2.

[0096] Wherein, the preparation method of the calendula extract comprises the following steps:

[0097] The marigolds are crushed into 100 meshes to obtain marigold powder, and the marigold powder, cellulase, and β-glucanase are added to water, and enzymolysis is performed at 50° C. for 4 hours, and filtering is performed; the mass ratio of the marigold powder, cellulase, β-glucanase, and water is 1:0.03:0.02:10;

[0098] The pH of the filtrate was adjusted to 6.6, and passed through AB-8 macroporous adsorption resin at a flow rate of 1 BV / h, the elution rate was 1 BV / h, 1.5 BV was first washed with water, and then 1.5 BV was washed with 80 wt% ethanol solution, the ethanol solution eluate was collected and dried to obtain a calendula extract.

[0099] Example 7

[0100] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0101] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:4:5.

[0102] The hyaluronic acid-based substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:0.25:0.5:0.25.

[0103] Wherein, the preparation method of the calendula extract comprises the following steps:

[0104] The marigolds are crushed into 100 meshes to obtain marigold powder, and the marigold powder, cellulase, and β-glucanase are added to water, and enzymolysis is performed at 50° C. for 4 hours, and filtering is performed; the mass ratio of the marigold powder, cellulase, β-glucanase, and water is 1:0.03:0.02:10;

[0105] The pH of the filtrate was adjusted to 6.6, and passed through AB-8 macroporous adsorption resin at a flow rate of 1 BV / h, the elution rate was 1 BV / h, 1.5 BV was first washed with water, and then 1.5 BV was washed with 80 wt% ethanol solution, the ethanol solution eluate was collected and dried to obtain a calendula extract.

[0106] Example 8

[0107] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0108] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:4:5.

[0109] The hyaluronic acid substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:2:4.5:2.5.

[0110] Wherein, the preparation method of the calendula extract comprises the following steps:

[0111] The marigolds are crushed into 100 meshes to obtain marigold powder, and the marigold powder, cellulase, and β-glucanase are added to water, and enzymolysis is performed at 50° C. for 4 hours, and filtering is performed; the mass ratio of the marigold powder, cellulase, β-glucanase, and water is 1:0.03:0.02:10;

[0112] The pH of the filtrate was adjusted to 6.6, and passed through AB-8 macroporous adsorption resin at a flow rate of 1 BV / h, the elution rate was 1 BV / h, 1.5 BV was first washed with water, and then 1.5 BV was washed with 80 wt% ethanol solution, the ethanol solution eluate was collected and dried to obtain a calendula extract.

[0113] Comparative Example 1

[0114] The difference between Comparative Example 1 and Example 1 is that the mass ratios of hyaluronic acid substances, peony root extract and calendula extract are different, and the others are the same.

[0115] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0116] The eye care composition comprises hyaluronic acid substances, peony root extract and calendula officinalis extract in a mass ratio of 1:0.2:0.8.

[0117] Comparative Example 2

[0118] The difference between Comparative Example 2 and Example 1 is that the mass ratios of hyaluronic acid substances, peony root extract and calendula extract are different, and the others are the same.

[0119] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0120] The eye care composition comprises hyaluronic acid substances, peony root extract and calendula officinalis extract in a mass ratio of 1:10:19.

[0121] Comparative Example 3

[0122] The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain hyaluronic acid substances, and the other aspects are the same.

[0123] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0124] The eye care composition comprises a peony root extract and a calendula extract in a mass ratio of 4:5.

[0125] Comparative Example 4

[0126] The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not contain peony root extract, and the other contents are the same.

[0127] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0128] The eye care composition comprises a hyaluronic acid substance and a marigold extract in a mass ratio of 1:5.

[0129] Comparative Example 5

[0130] The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain calendula extract, and the other contents are the same.

[0131] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0132] The eye care composition comprises a hyaluronic acid substance and a peony root extract in a mass ratio of 1:4.

[0133] Comparative Example 6

[0134] The difference between Comparative Example 6 and Example 1 is that the eye care composition is replaced by a hyaluronic acid substance in Comparative Example 6, and the other contents are the same.

[0135] An eye care solution comprises the following components in percentage by mass: 1.2% hyaluronic acid substances, 0.004% artificial bezoar, 0.01% folic acid, 0.05% ginseng extract, 0.1% menthone glycerol ketal, 0.2% dipeptide-2, 0.2% yeast polypeptides, 0.3% safflower extract, 0.4% lutein, 0.5% wormwood oil, 0.5% panthenol, 0.5% tocopherol, 4% glycerol, 0.1% Tween 20, and the balance water.

[0136] Comparative Example 7

[0137] The difference between Comparative Example 7 and Example 1 is that the eye care composition is replaced by peony root extract in Comparative Example 7, and the other contents are the same.

[0138] An eye care solution comprises the following components in percentage by mass: 1.2% peony root extract, 0.004% artificial bezoar, 0.01% folic acid, 0.05% ginseng extract, 0.1% menthone glycerol ketal, 0.2% dipeptide-2, 0.2% yeast polypeptides, 0.3% safflower extract, 0.4% lutein, 0.5% wormwood oil, 0.5% panthenol, 0.5% tocopherol, 4% glycerol, 0.1% Tween 20, and the balance water.

[0139] Comparative Example 8

[0140] The difference between Comparative Example 8 and Example 1 is that the eye care composition is replaced by the calendula extract in Comparative Example 8, and the other contents are the same.

[0141] An eye care solution comprises the following components in percentage by mass: 1.2% of calendula officinalis extract, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20 and the balance of water.

[0142] Comparative Example 9

[0143] The difference between Comparative Example 9 and Example 1 is that the hyaluronic acid substance in Comparative Example 9 is a single sodium hyaluronate cross-linked polymer.

[0144] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0145] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:4:5.

[0146] The hyaluronic acid substance is a sodium hyaluronate cross-linked polymer.

[0147] Comparative Example 10

[0148] The difference between Comparative Example 10 and Example 1 is that the hyaluronic acid substance in Comparative Example 10 is single hydrolyzed sodium hyaluronate.

[0149] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0150] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:4:5.

[0151] The hyaluronic acid substance is hydrolyzed sodium hyaluronate.

[0152] Comparative Example 11

[0153] The difference between Comparative Example 11 and Example 1 is that the hyaluronic acid substance in Comparative Example 11 is a single acetylated sodium hyaluronate.

[0154] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0155] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:4:5.

[0156] The hyaluronic acid substance is acetylated sodium hyaluronate.

[0157] Comparative Example 12

[0158] The difference between Comparative Example 12 and Example 1 is that the hyaluronic acid substance in Comparative Example 12 is single sodium hyaluronate.

[0159] An eye care solution comprises the following components in percentage by mass: 1.2% of an eye care composition, 0.004% of artificial bezoar, 0.01% of folic acid, 0.05% of ginseng extract, 0.1% of menthone glycerol ketal, 0.2% of dipeptide-2, 0.2% of yeast polypeptides, 0.3% of safflower extract, 0.4% of lutein, 0.5% of wormwood oil, 0.5% of panthenol, 0.5% of tocopherol, 4% of glycerol, 0.1% of Tween 20, and the balance of water.

[0160] The eye care composition comprises a hyaluronic acid substance, a peony root extract and a calendula officinalis extract in a mass ratio of 1:4:5.

[0161] The hyaluronic acid substance is sodium hyaluronate.

[0162] Comparative Example 13

[0163] The difference between Comparative Example 13 and Example 1 is that Comparative Example 13 uses an equal amount of Chuanxiong extract to replace the Calendula officinalis extract, and the other contents are the same.

[0164] Comparative Example 14

[0165] The difference between Comparative Example 14 and Example 1 is that Comparative Example 14 uses an equal amount of Salvia miltiorrhiza extract to replace Paeonia lactiflora root extract, and the rest are the same.

[0166] Test Case

[0167] Moisturizing and repairing efficacy test

[0168] Testing instruments: Corneometer CM 825 (CK electronic GmbH, Germany); Tewameter TM 300 (CK electronic GmbH, Germany).

[0169] Test subjects: 220 volunteers were selected and randomly divided into 22 groups, with 10 people in each group.

[0170] The criteria for inclusion of volunteers are: aged 20 to 28 years old, female; those who have not used hormone drugs and immunosuppressants in the past month; those who have not participated in other clinical trials at present or in the past 30 days; those who can cooperate well with the trial; those who can read and understand all the contents of the informed consent form and voluntarily sign the informed consent form; and those who agree not to use any cosmetics, drugs, and health products that may affect the results during the trial.

[0171] Exclusion criteria: those with open wounds or eroded surfaces; those with highly allergic constitutions; those with a history of cosmetic allergies; women who are pregnant, breastfeeding, or planning to become pregnant during the test period; those with severe heart, liver, and kidney damage and severe immunodeficiency; those with mental illness, severe endocrine diseases, and those taking oral contraceptives; those who have participated in drug clinical trials or other trials within 30 days, or those who have systemically used drugs that may affect the test results within the past week; those who have taken oral or external beauty products that may affect the test results within 2 weeks; those who are considered by the researchers to be unsuitable for participating in this test.

[0172] Suspension and exit criteria: Failure to apply the test sample according to the test requirements during the trial; suffering from serious illness or serious adverse reactions during use.

[0173] Test conditions: let the volunteers calm down in an environment with room temperature below 25℃±2℃ and relative humidity (RH) below 50%±2%. Expose the periocular area 5 minutes before the test, and measure the hydration degree of the periocular stratum corneum and transepidermal water loss (recorded as the initial value).

[0174] Test method: Apply 2 mL of the test sample to the tested area and massage until it is completely absorbed. Determine the moisture content of the stratum corneum at the same area 2 hours after using the test sample and the transepidermal moisture loss of the epidermis after use (recorded as the test value).

[0175] Skin hydration change rate = [(skin hydration test value - skin hydration initial value) / skin hydration initial value] * 100%.

[0176] TEWL change rate = [(TEWL test value - TEWL initial value) / TEWL initial value] * 100%.

[0177] Table 1

[0178]

[0179] As can be seen from Table 1, the eye care solution comprising the eye care composition of the present invention has excellent moisturizing and repairing effects, can effectively improve the problem of dry eye skin, relieve eye fatigue, and comprehensively improve eye conditions.

[0180] By comparing Example 1 with Comparative Examples 3 to 8 and Comparative Examples 13 and 14, it can be seen that the hyaluronic acid substances, peony root extracts and calendula extracts described in the present invention have significant synergistic effects. Under the synergistic effect of the three, the moisturizing and repairing effects are significantly improved.

[0181] By comparing Example 1 with Comparative Examples 9 to 12, it can be seen that the present invention adopts sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1: (0.25-4): (0.25-4): (0.5-1) as hyaluronic acid substances, which significantly improves the moisturizing and repairing effects.

[0182] By comparing Example 1 with Comparative Examples 1 to 2, it can be seen that the present invention significantly improves the moisturizing and repairing effects by controlling the mass ratio of hyaluronic acid substances, peony root extract and calendula extract to be 1: (0.5 to 8): (2 to 10).

[0183] It can be seen from the comparison of Examples 1 to 5 that the present invention further improves the moisturizing and repairing effects by controlling the mass ratio of hyaluronic acid substances, peony root extract and calendula extract to be 1: (2-5): (4-7).

[0184] From the comparison of Examples 1, 6 to 8, it can be seen that the present invention further improves the moisturizing and repairing effects by controlling the mass ratio of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate to sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate.

[0185] 2. Stability test

[0186] The eye care solutions of the embodiments and comparative examples were subjected to stability tests. The heat resistance test was conducted by placing the solution at 45°C for three months and observing its appearance, color and odor. The cold resistance test was conducted by placing the solution at -20°C for three months and observing its appearance, color and odor. The normal temperature test was conducted by placing the solution at 25°C for three months and observing its appearance, color and odor. The hot and cold cycle test was conducted by storing the solution at 45°C, room temperature and -20°C for 24 hours each, cycling the solution 10 times and observing its appearance, color and odor.

[0187] Table 2

[0188]

[0189]

[0190] It can be seen from Table 2 that the eye care solution comprising the eye care composition of the present invention has excellent stability.

[0191] 3. Irritation test

[0192] The test was carried out according to the method of SNT 2329-2009, and the results were evaluated by the stimulation scoring method.

[0193] The specific scores are shown in Table 3, and the evaluation results are shown in Table 4.

[0194] Table 3

[0195] Stimulus score Irritation classification IS<1 Non-irritating 1≤IS<5 Mild irritation 5≤IS<9 Moderate irritation IS ≥ 10 Strong irritant / corrosive

[0196] Table 4

[0197]

[0198]

[0199] It can be seen from Table 4 that the eye care solution of the present invention is mild and non-irritating and has good safety.

[0200] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An eye care composition, characterized in that The invention comprises hyaluronic acid, peony root extract and calendula officinalis extract in a mass ratio of 1:(0.5-8):(2-10); The sodium hyaluronate-like substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:(0.25-4):(0.25-4):(0.5-1).

2. The eye care composition according to claim 1, characterized in that The invention comprises hyaluronic acid substances, peony root extract and calendula officinalis extract in a mass ratio of 1:(2-5):(4-7).

3. The eye care composition according to claim 1, characterized in that The sodium hyaluronate-like substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1: (0.25-2): (0.5-4.5): (0.25-2.5).

4. The eye care composition according to claim 1, characterized in that The sodium hyaluronate-like substances include sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate in a mass ratio of 1:(0.5-1.5):(1.5-2.5):(1-2).

5. The eye care composition according to claim 1, characterized in that The preparation method of the calendula extract comprises the following steps: Adding marigold powder, cellulase and β-glucanase into water, performing enzymolysis and filtering; The pH value of the filtrate is adjusted to 6.2-6.8, and the filtrate is passed through a macroporous adsorption resin at a flow rate of 1-1.5 BV / h, and the elution rate is 1-1.5 BV / h. First, 1-1.5 BV is washed with water, and then 1-2 BV is washed with a 75-85 wt% ethanol solution. The ethanol solution eluate is collected and dried to obtain a calendula extract.

6. The eye care composition according to claim 5, characterized in that The mass ratio of the marigold pollen, cellulase, beta-glucanase and water is 1: (0.02-0.04): (0.01-0.02): (2-20).

7. The eye care composition according to claim 5, characterized in that The enzymolysis temperature is 48-55° C., and the enzymolysis time is 2-6 hours.

8. The eye care composition according to claim 5, characterized in that The macroporous adsorption resin is at least one of AB-8 macroporous adsorption resin, D101 macroporous adsorption resin, and HPD826 macroporous adsorption resin.

9. An eye care solution, characterized in that: The invention comprises the following components in percentage by mass: 0.5-1.5% of an eye care composition, 0.001-0.005% of artificial bezoar, 0.005-0.02% of vitamin B substances, 0.01-0.06% of ginseng substances, 0.05-0.2% of menthone glycerol acetal, 0.001-0.3% of dipeptide-2, 0.1-0.4% of yeast polypeptides, 0.1-0.5% of safflower extract, 0.01-0.5% of lutein, 0.01-0.6% of wormwood oil, 0.2-0.8% of panthenol, 0.01-0.6% of tocopherol, 2-5% of polyols, 0.04-1% of Tween 20, and the balance of water; The eye care composition is the eye care composition according to any one of claims 1 to 8.

10. The eye care solution according to claim 9, characterized in that: The ginseng substance comprises at least one of ginseng root water, ginseng extract, ginseng root extract, and hydrolyzed ginsenosides; and / or The polyol comprises at least one of glycerol, 1,2-propylene glycol, 1,3-propylene glycol, 1,3-butylene glycol, 1,4-butylene glycol, 1,2-pentanediol, and 1,5-pentanediol; and / or The vitamin B substances include at least one of biotin, folic acid, vitamin B12, vitamin B3, pantothenic acid, vitamin B6, vitamin B2, and vitamin B1.

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