Soothing and repairing composition for oil-sensitive skin and application thereof

Abstract

The invention provides a soothing and repairing composition for oil-sensitive skin and application of the soothing and repairing composition, and belongs to the technical field of cosmetics. According to the soothing and repairing composition, the hydrolyzed yeast protein, the aesculus chinensis extract, the marine oligosaccharide, the Australian tealeaf extract and the phellinus igniarius extract are compounded to form the soothing and repairing composition, and the soothing and repairing composition reduces skin discomfort by maintaining the steady state of an enzyme network of epidermis, reducing oxidase and increasing enzymes and defensive enzymes for promoting Newcastle metabolism; the effects of repairing, moisturizing, controlling oil and relieving are achieved, the skin injury of oily sensitive skin is gently repaired, and the health state of the skin is recovered.

Description

Technical Field

[0001] The present disclosure relates to the technical field of cosmetics, and in particular to a soothing and repairing composition for oily and sensitive skin and application thereof. Background Art

[0002] Oily sensitive skin is a type of skin that is both greasy and sensitive, characterized by excessive oil on the skin surface, which sometimes leads to a dull or uneven skin tone, and may be accompanied by blackheads and enlarged pores, making it prone to acne. Despite the presence of oil on the surface, oily sensitive skin may lack moisture inside, causing dryness and peeling in certain areas of the skin (such as the cheeks and nasolabial folds). Oily sensitive skin usually has skin barrier damage. This type of skin is extremely sensitive to external stimuli (such as climate change, fragrances, alcohol and other ingredients), and is prone to discomfort such as redness, stinging, itching or burning. Modern research has found that impaired stratum corneum function, especially the imbalance of stratum corneum lipids and water and protease imbalance, is one of the root causes of oily sensitive skin.

[0003] The epidermal enzyme network mainly includes a variety of enzymes involved in skin metabolism and barrier function, such as lipase, protease, glycosidase and oxidoreductase. These enzymes are responsible for the formation and renewal of the stratum corneum, regulating the balance of skin moisture and oil, and protecting the skin from external damage. Lipases promote the integrity of the skin barrier by breaking down lipids, while proteases help remove old dead skin cells and keep the skin smooth and breathable. The epidermal enzyme network is particularly important in the renewal and repair process of the skin. Normal enzyme activity can help regulate the metabolism of sebum, promote the shedding of dead skin cells, and maintain the health of the skin. If the function of the enzyme is affected, it may lead to the accumulation of the stratum corneum of the skin, increase the secretion of oil, and form a vicious circle.

[0004] In the existing technology, the care for oily skin mainly focuses on oil control, anti-inflammation, etc., and does not take care of oily skin from the aspect of epidermal enzyme network homeostasis. Most of the powerful oil control and anti-inflammatory agents have the problem of high irritation under high efficacy. Summary of the invention

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a soothing and repairing composition for oily-sensitive skin and its application.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In the first aspect, a soothing and repairing composition for oily and sensitive skin is provided, comprising the following components in parts by weight: 1-5 parts of hydrolyzed yeast protein, 1-3 parts of horse chestnut extract, 0.5-2 parts of marine oligosaccharides, 1-3 parts of broom leaf Australian tea extract, and 1-3 parts of medicinal pore fungus extract.

[0007] In some embodiments, the following components are included in parts by weight: 2-3 parts of hydrolyzed yeast protein, 1.5-2 parts of horse chestnut extract, 1-1.5 parts of marine oligosaccharides, 1.5-2 parts of broom leaf Australian tea extract, and 1-3 parts of medicinal pore fungus extract.

[0008] In some embodiments, the preparation method of the broom leaf Australian tea leaf extract is: adding the broom leaf Australian tea leaf to a low eutectic solvent, extracting at a temperature of 50-80°C for 2-4 hours, separating the solid and the liquid, and the obtained liquid is the broom leaf Australian tea leaf extract, wherein the hydrogen bond acceptor of the low eutectic solvent is glycerol glucoside, and the hydrogen bond donor is bosine or allantoin.

[0009] In some embodiments, the mass ratio of the hydrogen bond donor to the hydrogen bond acceptor is 1:1-1:2.

[0010] In some embodiments, the mass ratio of the Australian tea leaves to the deep eutectic solvent is 1:5-10.

[0011] In a second aspect, a use of the soothing and repairing composition in the preparation of cosmetics is provided.

[0012] In a third aspect, a cosmetic is provided, the cosmetic comprising the soothing and repairing composition, wherein the mass percentage of the soothing and repairing composition in the cosmetic is 0.5-10%.

[0013] In some embodiments, the cosmetic is at least one of lotion, emulsion, cream, mask, essence, and spray.

[0014] In some embodiments, the cosmetic is a facial cream, and the facial cream includes the following components in percentage by weight: 0.5-10% soothing and repairing composition, 0.05-0.3% thickener, 0.5-1% moisturizer, 0.5-5% emulsifier, 0.5-3% preservative, 0.01-0.3% pH adjuster, and the remainder is water.

[0015] In some embodiments, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0016] And / or, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, 1,3-propylene glycol, glycerol, budding septin polysaccharide, and ceramide;

[0017] And / or, the pH regulator includes at least one of arginine, tromethamine, and EDTA-disodium;

[0018] and / or, the emulsifier comprises at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane / polysiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0019] And / or, the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention compounds hydrolyzed yeast protein, horse chestnut extract, marine oligosaccharides, broom leaf Australian tea extract and medicinal pore fungus extract to form a soothing and repairing composition, which reduces skin discomfort by maintaining the homeostasis of the epidermal enzyme network, reducing oxidase, and increasing enzymes that promote new city metabolism and defense enzymes, thereby achieving the effects of repairing, moisturizing, controlling oil and soothing, gently repairing skin damage of oily sensitive skin, and restoring the skin to a healthy state. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 These are the oil control, soothing and repairing effects on the human body of Application Example 1 and Comparative Application Example. DETAILED DESCRIPTION

[0022] In order to facilitate understanding of the present disclosure, the present disclosure will be described more fully below. However, the present disclosure can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present disclosure more thorough and comprehensive.

[0023] As used herein:

[0024] "Prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0025] The conjunction "consisting of excludes any unspecified element, step, or component. If used in a claim, this phrase renders the claim closed-ended so that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the body of a claim rather than immediately following the subject matter, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.

[0026] When amount, concentration or other value or parameter is represented by range, preferred range or a series of upper preferred value and lower preferred value, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1-5", described range should be interpreted as including range "1-4", "1-3", "1-2", "1-2 and 4-5", "1-3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.

[0027] In these examples, parts and percentages are by mass unless otherwise indicated.

[0028] "Parts by mass" refers to the basic unit of measurement for expressing the mass ratio of multiple components. 1 part can represent any unit mass, such as 1g or 2.689g. If we say that the mass of component A is a parts and the mass of component B is b parts, it means the ratio of the mass of component A to the mass of component B is a:b. Alternatively, it means that the mass of component A is aK and the mass of component B is bK (K is an arbitrary number, indicating a multiple factor). It should not be misunderstood that, unlike the mass parts, the sum of the mass of all components is not limited to 100 parts.

[0029] “And / or” is used to indicate that one or both of the stated situations may occur, for example, A and / or B includes (A and B) and (A or B).

[0030] In a first aspect, a soothing and repairing composition for oily sensitive skin is provided, comprising the following components in parts by weight: 1-5 parts of hydrolyzed yeast protein, 1-3 parts of horse chestnut extract, 0.5-2 parts of marine oligosaccharides, 1-3 parts of broom leaf Australian tea extract, and 1-3 parts of medicinal pore fungus extract.

[0031] In some embodiments, the following components are included in parts by weight: 2-3 parts of hydrolyzed yeast protein, 1.5-2 parts of horse chestnut extract, 1-1.5 parts of marine oligosaccharides, 1.5-2 parts of broom leaf Australian tea extract, and 1-3 parts of medicinal pore fungus extract.

[0032] For example, in different embodiments, the weight portion of the hydrolyzed yeast protein can be, but is not limited to, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts; preferably 2-3 parts;

[0033] For example, in different embodiments, the weight portion of the horse chestnut extract may be, but is not limited to, 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, 3 parts; preferably 1.5-2 parts;

[0034] For example, in different embodiments, the weight portion of the marine oligosaccharide may be, but is not limited to, 0.5, 0.7, 0.9, 1.1, 1.3, 1.5, 1.7, 1.9, or 2; preferably 1.5-2;

[0035] For example, in different embodiments, the weight portion of the broom leaf Australian tea extract can be, but is not limited to, 1 part, 1.3 parts, 1.5 parts, 1.7 parts, 1.9 parts, 2.1 parts, 2.3 parts, 2.5 parts, 2.7 parts, 3 parts; preferably 1.5-2 parts;

[0036] For example, in different embodiments, the weight proportion of the medicinal pore fungus extract may be, but is not limited to, 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, or 3 parts.

[0037] The present invention compounds hydrolyzed yeast protein, horse chestnut extract, marine oligosaccharide, broom leaf Australian tea extract and medicinal pore fungus extract to form a soothing and repairing composition; in the first aspect, the soothing and repairing composition can increase cutinase and lipase, reduce hyaluronidase to regulate the skin barrier function, ensure the formation and renewal of the stratum corneum, thereby preventing water loss and the invasion of external harmful substances; at the same time, the water and oil balance of the skin is maintained, and the absorption of nutrients is related to the discharge of waste. In the second aspect, the soothing and repairing composition of the present invention can also promote antioxidant enzymes, such as superoxide dismutase (SOD), inhibit matrix metalloproteinases (MMPs), thereby reducing skin inflammatory reactions, restoring the function of skin immune regulation, regulating intercellular signal transmission and activating immune cells, and promoting the skin damage repair process. In short, the composition of the present invention not only ensures the physiological function of the skin by maintaining the homeostasis of the skin enzyme network, but also helps to soothe and repair damaged tissues, reduce excessive oil secreted by oily skin, and maintain skin health.

[0038] Specifically, the hydrolyzed yeast protein refers to yeast protein treated by a hydrolysis process, usually a mixture of small molecule peptides and amino acids. Hydrolyzed yeast protein rich in amino acids supports and regulates sebum secretion, helps maintain the epidermal enzyme network of oily sensitive skin, and prevents pore blockage and inflammation. It enhances the skin barrier function, reduces redness and itching caused by external stimuli, and stimulates the activity of certain enzymes, promotes keratin health, and reduces the occurrence of acne and blackheads. Its antioxidant properties can also reduce oxidative stress, improve the overall condition of oily sensitive skin and maintain health.

[0039] Horse chestnut extract is rich in saponin compounds, which have the effect of oil control and grease removal, and is particularly beneficial for oily and sensitive skin. It relieves skin erythema, edema and allergies through anti-inflammatory effects, and promotes metabolism and enhances skin elasticity. In addition, horse chestnut extract can inhibit the activity of enzymes that damage vascular structure, improve microcirculation, reduce red blood streaks and eye puffiness, and provide comprehensive skin care effects for oily and sensitive skin.

[0040] Marine oligosaccharides form a protective film through its rich hydroxyl structure, effectively locking in moisture and providing long-lasting moisturizing. It has good antioxidant effects, can neutralize free radicals, slow down skin aging, and enhance skin immunity. In addition, marine oligosaccharides can soothe the skin, reduce problem reactions, and promote skin cell regeneration, improve elasticity, and reduce fine lines. By inhibiting the production of melanin, marine oligosaccharides also have excellent whitening effects, ensuring the overall health of oily and sensitive skin in terms of moisturizing, whitening, and repairing.

[0041] Broom Leaf Australian Tea Extract has significant antioxidant, anti-inflammatory and moisturizing effects, suitable for oily and sensitive skin. Its rich antioxidants can neutralize free radicals, repair damaged skin and slow down the aging process. At the same time, the natural anti-inflammatory properties can effectively relieve skin inflammation caused by oxidation and UV rays, and reduce sensitive symptoms. In addition, the extract can enhance the skin's ability to retain moisture, promote even distribution of moisture, and improve skin softness and elasticity.

[0042] The medicinal pore fungus extract regulates the epidermal enzyme network through multiple mechanisms. Its triterpenoid bioactive ingredients can promote the expression and activity of antioxidant enzymes and key metabolic enzymes, and improve skin metabolism. At the same time, the extract can regulate the enzyme activity related to water-oil balance, has good oil control ability, enhances the repair ability of the skin barrier, and maintains water-lipid balance. In addition, its anti-inflammatory effect helps to inhibit the activity of inflammation-related enzymes, thereby maintaining skin health and vitality.

[0043] In some embodiments, the preparation method of the broom leaf Australian tea leaf extract is: adding the broom leaf Australian tea leaf to a low eutectic solvent, extracting at a temperature of 50-80°C for 2-4 hours, separating the solid and the liquid, and the obtained liquid is the broom leaf Australian tea leaf extract, wherein the hydrogen bond acceptor of the low eutectic solvent is glycerol glucoside, and the hydrogen bond donor is bosine or allantoin.

[0044] The present invention uses glycerol glucoside as a hydrogen bond acceptor and bosine or allantoin as a hydrogen bond donor to form a low eutectic solvent. The obtained low eutectic solvent is used to extract broom leaf Australian tea. On the one hand, it can effectively increase the content of active ingredients in the broom leaf Australian tea extract, thereby improving the repair, moisturizing, oil control and soothing effects of the soothing repair composition; on the other hand, the hydrogen bond donor can improve the biocompatibility of the soothing repair composition and improve the anti-aging effect of the soothing repair composition; the hydrogen bond acceptor can improve the antioxidant and moisturizing effects of the soothing repair composition and maintain the water-oil balance of the skin.

[0045] For example, in different embodiments, the extraction temperature may be, but is not limited to, 50°C, 52°C, 54°C, 56°C, 58°C, 60°C, 62°C, 64°C, 66°C, 68°C, 70°C, 72°C, 74°C, 76°C, 78°C, 80°C;

[0046] For example, in different embodiments, the extraction time can be, but is not limited to, 2 h, 2.5 h, 3 h, 3.5 h, or 4 h.

[0047] In some embodiments, the mass ratio of the hydrogen bond donor to the hydrogen bond acceptor is 1:1-1:2, for example, but not limited to 1:1, 1:1.2, 1:1.4, 1:1.6, 1:1.8, 1:2.

[0048] In some embodiments, the mass ratio of the Australian tea leaves to the deep eutectic solvent is 1:5-10, for example, but not limited to 1:5, 1:6, 1:7, 1:8, 1:9, 1:10.

[0049] The present disclosure study found that within the above parameter range, the content of effective active ingredients in the Australian tea leaf extract can be further increased, thereby improving the repairing, moisturizing, oil-controlling and soothing effects of the soothing repair composition.

[0050] Specifically, in order to further improve the effective active ingredient content and extraction efficiency of the Australian Broom Leaf Tea extract, the average particle size of the Australian Broom Leaf Tea is less than 80 meshes.

[0051] Specifically, the preparation method of the broom leaf Australian tea powder with an average particle size of less than 80 meshes is: wash the broom leaf Australian tea with water, freeze-dry it at -80°C for 1 hour, grind it, and pass it through an 80-mesh sieve to obtain the powder.

[0052] Specifically, there is no limitation on the method of solid-liquid separation, as long as the solid and liquid can be separated, such as centrifugation, filtration, suction filtration, etc. The present invention prefers suction filtration as the method of solid-liquid separation.

[0053] Specifically, the preparation method of the low eutectic solvent is not specifically limited in the present invention. The low eutectic solvent can be prepared by known methods in the art. For example, the hydrogen bond donor and the hydrogen bond acceptor are stirred at a temperature of 50-80° C. to obtain a low eutectic solvent. The stirring time is not specifically limited. It is sufficient to determine that the melting point of the low eutectic solvent is lower than the melting point of the hydrogen bond donor or the hydrogen bond acceptor. For example, the stirring time can be 1h-3h.

[0054] In the present invention, hydrolyzed yeast protein, horse chestnut extract, marine oligosaccharide and medicinal porphyra extract can be prepared according to methods known in the art, or obtained through commercial channels.

[0055] In a second aspect, a use of the soothing and repairing composition in the preparation of cosmetics is provided.

[0056] In a third aspect, a cosmetic is provided, comprising the soothing and repairing composition, wherein the mass percentage of the soothing and repairing composition in the cosmetic is 0.5-10%, for example, it may be but not limited to 0.5%, 0.7%, 1%, 1.2%, 1.5%, 1.7%, 2%, 2.3%, 2.5%, 2.8%, 3%, 3.2%, 3.5%, 3.7%, 4%, 4.3%, 4.5%, 4.8%, 5%, 6%, 7%, 8%, 9%, 10%.

[0057] In addition to the above-mentioned extracts, the composition used according to the invention generally comprises a physiologically acceptable and preferably cosmetically acceptable medium, meaning a medium suitable for contact with human skin without toxicity, incompatibility, instability or allergic reaction, in particular without causing discomfort (redness, tightness, stinging). Advantageously, the cosmetic can be in the form of a powder, emulsion, microemulsion, nanoemulsion, suspension, solution, lotion, cream, aqueous or hydroalcoholic gel, foam, solution or dispersion for aerosol or lipid vesicle dispersion. In the case of an emulsion, it can be a water-in-oil or oil-in-water emulsion.

[0058] In some embodiments, the cosmetic is at least one of lotion, emulsion, cream, mask, essence, and spray.

[0059] In some embodiments, the cosmetic is a facial cream, and the facial cream includes the following components in percentage by weight: 0.5-10% soothing and repairing composition, 0.05-0.3% thickener, 0.5-1% moisturizer, 0.5-5% emulsifier, 0.5-3% preservative, 0.01-0.3% pH adjuster, and the remainder is water.

[0060] For example, in different embodiments, the mass percentage of the soothing and repairing composition can be, but is not limited to, 0.5%, 0.7%, 1%, 1.2%, 1.5%, 1.7%, 2%, 2.3%, 2.5%, 2.8%, 3%, 3.2%, 3.5%, 3.7%, 4%, 4.3%, 4.5%, 4.8%, 5%, 6%, 7%, 8%, 9%, 10%;

[0061] For example, in different embodiments, the mass percentage of the thickener may be, but is not limited to, 0.05%, 0.07%, 0.1%, 0.12%, 0.15%, 0.2%, 0.23%, 0.25%, 0.28%, 0.3%;

[0062] For example, in different embodiments, the mass percentage of the moisturizing agent may be, but is not limited to, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%;

[0063] For example, in different embodiments, the mass percentage of the emulsifier may be, but is not limited to, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%;

[0064] For example, in different embodiments, the mass percentage of the preservative may be, but is not limited to, 0.5%, 0.6%, 0.8%, 1%, 1.5%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%;

[0065] For example, in different embodiments, the mass percentage of the pH adjuster may be, but is not limited to, 0.05%, 0.07%, 0.1%, 0.15%, 0.2%, 0.25%, or 0.3%.

[0066] In some embodiments, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0067] In some embodiments, the moisturizer includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, 1,3-propylene glycol, glycerol, budding septin polysaccharide, and ceramide;

[0068] In some embodiments, the pH adjuster includes at least one of arginine, tromethamine, and EDTA-disodium;

[0069] In some embodiments, the emulsifier includes at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane / polysilicone, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0070] In some embodiments, the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, and phenoxyethanol.

[0071] The cosmetics of the present invention can be prepared by any suitable method known in the art. For example, the cosmetics can be prepared using equipment such as a dissolving tank, an emulsifying pot, a disperser, and a delivery pump commonly used in the cosmetics field according to processes known in the art. For example, a water-soluble substance can be first put into an aqueous phase dissolving pot, and an oil-soluble substance (such as a sunscreen, grease) can be put into an oil phase dissolving pot, and the temperature of the two pots can be heated to about 80°C, respectively. For raw materials that are easy to agglomerate, they can be pre-dispersed with a disperser; such as powder-containing substances (such as zinc oxide and titanium dioxide), they can be dispersed with a suitable solvent and a dispersant and homogenized for 5-10 minutes, and the oil phase is added and homogenized again for 5-10 minutes; after homogenization is completed, the oil phase and the water phase are delivered to the emulsifying pot for homogenization and emulsification for about 5-15 minutes; after emulsification is completed, the temperature of the material body is reduced to room temperature, and optionally, flavors, preservatives, etc. are added, and the pH of the product is adjusted as needed; after the relevant test indicators are qualified, the material can be filled and discharged.

[0072] The above preparation process is merely exemplary, and those skilled in the art can increase, decrease or adjust it according to the dosage form requirements to prepare various dosage forms such as spray, emulsion, gel / jelly, cream, cushion / foundation, etc.

[0073] In order to further illustrate the present invention, the soothing and repairing composition for oily-sensitive skin and its application provided by the present invention are described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0074] The raw materials used in the embodiments and comparative examples are described below, but are not limited to these materials:

[0075] Broom leaf Australian tea extract-1 is homemade, and its preparation method is as follows:

[0076] Stirring bosacein and glycerol glucoside at a molar ratio of 1:1.5 at 60°C for 2 hours to obtain a first solvent, adding ultrapure water, stirring evenly, and obtaining a low eutectic solvent, wherein the mass of the first solvent is 50% of the mass of the low eutectic solvent;

[0077] Adding broom leaf Australian tea powder with an average particle size of less than 80 meshes into a low eutectic solvent, extracting at a temperature of 70°C for 3 hours, filtering, and obtaining a filtrate as broom leaf Australian tea extract-1, wherein the mass ratio of broom leaf Australian tea powder to the low eutectic solvent is 1:8.

[0078] Broom leaf Australian tea extract-2 is homemade, and its preparation method is as follows:

[0079] Stirring bosaicin and glycerol glucoside in a molar ratio of 1:1 at a temperature of 50° C. for 3 hours to obtain a first solvent, adding ultrapure water, and stirring evenly to obtain a low eutectic solvent, wherein the mass of the first solvent is 50% of the mass of the low eutectic solvent;

[0080] Adding broom leaf Australian tea powder with an average particle size of less than 80 meshes into a low eutectic solvent, extracting at a temperature of 50°C for 3 hours, filtering, and obtaining a filtrate as broom leaf Australian tea extract-2, wherein the mass ratio of broom leaf Australian tea powder to the low eutectic solvent is 1:10.

[0081] Broom leaf Australian tea extract-3 is homemade, and its preparation method is as follows:

[0082] Stirring bosacein and glycerol glucoside in a molar ratio of 1:2 at 80° C. for 1 hour to obtain a first solvent, adding ultrapure water, and stirring evenly to obtain a low eutectic solvent, wherein the mass of the first solvent is 50% of the mass of the low eutectic solvent;

[0083] Adding broom leaf Australian tea powder with an average particle size of less than 80 meshes into a low eutectic solvent, extracting at a temperature of 80°C for 3 hours, filtering, and obtaining a filtrate as broom leaf Australian tea extract-3, wherein the mass ratio of broom leaf Australian tea powder to the low eutectic solvent is 1:5.

[0084] Broom Leaf Australian Tea Extract-4 is homemade, and its preparation method differs from that of Broom Leaf Australian Tea Extract-1 only in that allantoin is used instead of benzoin.

[0085] Broomrape Australia Tea Extract-5 is homemade, and its preparation method differs from Broomrape Australia Tea Extract-1 only in that betaine is used instead of benzoin.

[0086] Broomrape Leaf Australian Tea Extract-6 is homemade, and its preparation method differs from that of Broomrape Leaf Australian Tea Extract-1 only in that glycerol is used instead of glyceryl glucoside.

[0087] Broom leaf Australian tea leaf extract-7 is homemade, and its preparation method is different from that of broom leaf Australian tea leaf extract-1 only in that ethanol is used to replace the low eutectic solvent.

[0088] Broom leaf Australian tea leaf extract-8 is homemade, and its preparation method is different from that of broom leaf Australian tea leaf extract-1 only in that water is used to replace the low eutectic solvent.

[0089] Examples and Comparative Examples

[0090] The present invention provides a repair composition according to the embodiment and comparative example. The components of the repair composition are shown in Table 1 and Table 2.

[0091] The preparation method of the repair composition of the embodiment and comparative example is:

[0092] Weigh the components according to the weight parts in Table 1 and Table 2, and mix the components evenly to obtain the soothing and repairing composition.

[0093] Table 1

[0094]

[0095]

[0096] Table 2

[0097]

[0098] Application examples 1-15, comparative application examples 1-10 and blank application examples

[0099] The application examples, comparative application examples and blank application examples of the present invention provide a facial cream, the components (mass percentage) of the facial cream are shown in Table 3; wherein the soothing repairing compositions used in application examples 1-12 are respectively the soothing repairing compositions prepared in embodiments 1-12, and the soothing repairing compositions used in comparative application examples 1-10 are respectively the soothing repairing compositions prepared in comparative examples 1-10; the soothing repairing compositions used in application examples 13-15 are the soothing repairing compositions prepared in embodiment 1;

[0100] The preparation methods of the facial creams of the application examples, comparative application examples and blank application examples are as follows:

[0101] (1) Mixing a humectant, a thickener and water, heating to 85° C., homogenizing at a speed of 1200 rpm for 4 minutes, and keeping the mixture warm for later use to obtain a prefabricated component A;

[0102] (2) mixing the emulsifier, heating to 85° C., homogenizing at 1200 rpm for 4 min, and keeping warm for later use to obtain prefabricated component B;

[0103] (3) mixing the preservatives and heating to 60° C. to melt to obtain a prefabricated C component;

[0104] (4) The preformed component A is heated to 80°C, and the preformed component B is added at a speed of 250 rpm, and the mixture is stirred and mixed evenly. Then, the temperature is lowered to 60°C, and the preformed component C is added at a speed of 250 rpm and stirred and mixed. Then, the temperature is lowered to below 40°C, and the soothing repair composition is added and stirring is continued for 5 minutes. Finally, a pH adjuster is added to adjust the pH to 6.0, and stirring is stopped. The material is discharged to obtain a facial cream.

[0105] Table 3

[0106]

[0107] Effect Example 1

[0108] This effect example explores the effect of the soothing and repairing compositions prepared in Examples 1-12 and Comparative Examples 1-10 on the homeostasis of epidermal enzymes.

[0109] 1. The effects of the soothing and repairing compositions prepared in Examples 1-12 and Comparative Examples 1-10 on superoxide dismutase were tested by in vitro tests.

[0110] Superoxide dismutase (SOD) is one of the main antioxidant enzymes and has multiple benefits for the skin. It can effectively remove superoxide anions from the body, reduce oxidative stress, slow down skin aging, and improve cell health. By protecting cell membranes and proteins, SOD helps maintain the structure and function of the skin. At the same time, it also plays an important role in maintaining the epidermal enzyme network, promoting the activity of other antioxidant enzymes, improving the metabolism of the skin, enhancing the skin barrier function, and helping the skin stay young and healthy. The activity of the SOD enzyme can be tested in vitro using a photoreduction reaction. The darker the blue color of the reaction solution, the lower the activity of the SOD enzyme produced by the composition, and the lower the antioxidant capacity. Conversely, the higher the antioxidant capacity, the activity and antioxidant capacity of the SOD enzyme of the composition can be calculated based on this.

[0111] The experimental materials are as follows:

[0112] 130mmol / L methionine (Met) solution: weigh 1.9389g Met and dissolve it in phosphate buffer to make up to 100ml; store in a refrigerator at 4℃ for 1 to 2 days;

[0113] 750μmol / L nitroblue tetrazolium (NBT) solution: weigh 0.06133g NBT and dissolve it in phosphate buffer to 100ml, store in dark place; store in a refrigerator at 4℃ for 2-3 days;

[0114] 20μmol / L riboflavin solution: weigh 0.0075g riboflavin, dissolve it in phosphate buffer to make up to 1000mL, prepare and use immediately, and store in dark place;

[0115] 100μmol / L EDTA-2Na solution: weigh 0.0372g EDTA-2Na and dilute to 1000mL with distilled water;

[0116] Sample solution: Add sterile PBS buffer to the soothing repair composition obtained in each example and comparative example in Table 1 until the total mass of the sample solution is 100 parts by weight. Taking Example 1 as an example, 90.7 parts by weight of sterile PBS buffer is added to the soothing repair composition of Example 1 to obtain the sample solution of Example 1.

[0117] The test steps are as follows:

[0118] Take 5 clean and dried transparent EP tubes from each group, number them, add various reagents and enzyme solution according to Table 4, and the total volume of the reaction system is 3mL; after vortex mixing, each tube is reacted for 10min at different temperatures (20℃, 25℃, 35℃, 40℃) and 4000-4500lx sunlight (the light conditions of each tube are required to be consistent, and the reaction time is adjusted appropriately according to the reaction color of the control tube under light and the level of enzyme activity), and tube No. 5 is kept at the same temperature but protected from light; after the reaction is completed, cover it with a black cloth, terminate the reaction and use tube No. 5 as a blank to adjust to zero, measure the absorbance of the reaction solution in tubes 1-4 at 560nm, record the measurement data, and repeat each experiment 3 times.

[0119] Table 4 Sample tube configuration table

[0120]

[0121]

[0122] Calculation formula:

[0123] The ability of the sample to inhibit superoxide anions is represented by SOD-like enzyme activity. It is known that the SOD activity unit is represented by 50% inhibition of NBT photochemical reduction as one enzyme activity unit. The SOD activity of the soothing repair composition obtained in each embodiment and comparative example is calculated according to Formula 1:

[0124] Total SOD activity (U / mL) = (A0-As) × V / 50% × A0 × Vs (Formula 1),

[0125] The total SOD activity is expressed as enzyme units per mL of sample (U / mL).

[0126] In formula 1, A0: absorbance of control tube under light; As: absorbance of sample measuring tube; V: total volume of sample extract (mL); Vs: volume of sample extract taken during measurement (mL). Because this study detects the SOD enzyme activity per mL of the composition rather than inferring the SOD enzyme activity of the raw material through the extract, V and Vs can offset each other, and the final calculation formula is: SOD total activity (U / mL) = (A0-As) / 50%×A0, and the test results are shown in Table 5.

[0127] 2. Cell experiments were used to test the effects of the soothing and repairing compositions prepared in Examples 1-12 and Comparative Examples 1-10 on KLK5 protein.

[0128] KLK5 protein is an important keratinase that has significant benefits in promoting the metabolism of keratinocytes. It promotes the removal of dead skin cells by regulating the exfoliation and renewal process of the stratum corneum, and helps to generate new keratinocytes, thereby keeping the skin smooth and soft. In addition, KLK5 is also involved in regulating the skin barrier function and enhancing the skin's defense capabilities by promoting metabolism. Oily and sensitive skin requires proper keratin exfoliation, which can promote skin metabolism and keep the skin healthy. By measuring the ability of the composition to promote KLK5 protein, the effect of the composition on maintaining the homeostasis of the epidermal enzyme network can be determined.

[0129] The test steps are as follows:

[0130] Human keratinocytes (HaCaT cells) were revived and cultured with MEM containing 10% FBS as a culture medium. The soothing composition examples / comparative examples were diluted with cell MEM culture medium to prepare a working solution. The sample group discarded the cell culture medium, added different soothing composition examples / comparative examples to treat HaCaT cells for 48 hours, and the cells without soothing composition and UV irradiation were used as blank controls. Different soothing repair compositions were configured as sample solutions and co-cultured with HaCaT cells, and the cells were harvested and the concentration of KLK5 protein in HaCaT cells was detected by ELISA kit.

[0131] The calculation formula is: KLK5 protein promotion rate (%) = (sample group content - blank group content) / blank group content × 100%. The test results are shown in Table 5.

[0132] 3. Cell experiments were used to test the effects of the soothing and repairing compositions prepared in Examples 1-12 and Comparative Examples 1-10 on matrix metalloproteinase MMP-9.

[0133] MMP-9 (matrix metalloproteinase-9) plays an important role in skin damage, and its main function is to decompose collagen and other extracellular matrix components. Skin damage or external stimulation can lead to excessive MMP-9 activity, which in turn leads to the destruction of skin structure, damages elasticity and firmness, and accelerates the aging process. In addition, MMP-9 can also destroy the epidermal enzyme network, interfere with the normal function of the enzyme, cause damage to the skin barrier and aggravate inflammation, weaken skin resistance, and cause sensitivity and other problems. MMP-9 enzyme is an indispensable component of the epidermal enzyme network. By combining the ability to inhibit matrix metalloproteinase MMP-9, the effect of the composition on maintaining the homeostasis of the epidermal enzyme network can be determined.

[0134] The test steps are as follows:

[0135] Human keratinocytes (HaCaT cells) were revived and cultured in MEM containing 10% FBS. 2 , 320nm irradiation simulates skin damage. The soothing composition examples / comparative examples were diluted with cell MEM culture medium to prepare a working solution. The cell culture solution was discarded from the sample group, and different soothing composition examples / comparative examples were added to treat HaCaT cells for 48 hours, and the cells without soothing composition and UV irradiation were used as the blank control group. Cells that were simply irradiated with UV rays but without the addition of soothing repair compositions were used as the model group. Different soothing repair compositions were configured into sample solutions and co-cultured with HaCaT cells, and then the cells were harvested and the concentration of MMP-9 protease in HaCaT cells was detected by ELISA kit.

[0136] The calculation formula is: MMP-9 protease inhibition rate (%) = (model group content - sample group content) / model group content × 100%. The test results are shown in Table 5.

[0137] Table 5

[0138]

[0139]

[0140] From the experimental data in Table 5, it can be seen that using the soothing repair composition of the present invention, the total SOD activity reaches more than 69U / mL, the KLK5 promotion rate reaches more than 37%, and the MMP-9 inhibition rate reaches more than 57%, which indicates that the soothing repair composition of the present invention can promote superoxide dismutase and KLK5 protein, inhibit matrix metalloproteinase-9, and effectively maintain the homeostasis of the epidermal enzyme network.

[0141] From comparative examples 1-5, it can be seen that when the soothing repair composition comprises the following components in parts by weight: 2-3 parts of hydrolyzed yeast protein, 1.5-2 parts of horse chestnut extract, 1-1.5 parts of marine oligosaccharides, 1.5-2 parts of broom leaf Australian tea extract, and 1-3 parts of medicinal pore fungus extract, the obtained soothing repair composition is used, and the total SOD activity reaches more than 80U / mL, the KLK5 promotion rate reaches more than 42%, and the MMP-9 inhibition rate reaches more than 63%; it indicates that the soothing repair composition within this range has a better promoting effect on superoxide dismutase and KLK5 protein, and a better inhibitory effect on matrix metalloproteinase-9, which is beneficial to maintaining the homeostasis of the epidermal enzyme network.

[0142] By comparing Example 1 with Examples 6-12, it can be seen that the broom leaf Australian tea extract is extracted using a low eutectic solvent composed of glycerol glucoside as a hydrogen bond acceptor and bosine or allantoin as a hydrogen bond donor. Using the soothing repair composition containing the broom leaf Australian tea extract, the total SOD activity reaches more than 102U / mL, the KLK5 promotion rate reaches more than 47%, and the MMP-9 inhibition rate reaches more than 72%; it indicates that the soothing repair composition within this range has a better promoting effect on superoxide dismutase and KLK5 protein, and a better inhibitory effect on matrix metalloproteinase-9, which is beneficial to maintaining the homeostasis of the epidermal enzyme network.

[0143] By comparing Example 1 and Comparative Examples 1-5, it can be seen that by using only one of hydrolyzed yeast protein, horse chestnut extract, marine oligosaccharides, broom leaf Australian tea extract and medicinal pore fungus extract, or using other analogs to replace one of hydrolyzed yeast protein, horse chestnut extract, marine oligosaccharides, broom leaf Australian tea extract and medicinal pore fungus extract, and using the obtained soothing repair composition, the total SOD activity is lower than 65U / mL, the KLK5 promotion rate is lower than 35%, and the MMP-9 inhibition rate is lower than 55%; this indicates that there is a synergistic effect between hydrolyzed yeast protein, horse chestnut extract, marine oligosaccharides, broom leaf Australian tea extract and medicinal pore fungus extract, which effectively improves the effect of the soothing repair composition in maintaining the homeostasis of the epidermal enzyme network.

[0144] Effect Example 2 Human Efficacy Test

[0145] This effect example tests the soothing, repairing, moisturizing and oil-control effects of the creams of Application Examples 1-15, Comparative Application Examples 1-10 and Blank Application Examples on oily sensitive skin.

[0146] 104 subjects aged 28-60 years old with oily skin (sebum content > 120ug / cm 2) Asian adult testers with sensitive skin (lactic acid positive) were randomly divided into 26 groups, 4 people in each group. The volunteers used the samples (creams prepared by application examples 1-15, comparative application examples 1-10 and blank application examples) on their entire faces once in the morning and evening every day, and visited the site to collect data on the 0th and 28th days of use;

[0147] After the visit, the volunteers washed their faces with facial cleanser and sat in an air-conditioned room at a temperature of 21±1℃ and a humidity of 50±10% for 2 hours. The researchers used the skin moisture loss test probe TewaMeter to measure the transepidermal water loss value TEWL of the zygomatic bone, the skin moisture content of the zygomatic bone using the skin moisture test probe Corneometer, and the sebum content of the forehead using the sebum test probe SebumeterSM 815. A 4-point self-assessment questionnaire was used to evaluate the volunteers' skin erythema, itching, tingling, burning and other discomforts (the higher the score, the stronger the discomfort, 0=none to 3=severe), and Visia CR was used to take facial images of the subjects.

[0148] The skin barrier repair ability is represented by the improvement of TEWL value, the moisturizing effect is represented by the improvement of skin moisture content, the oil control effect is represented by the improvement of skin sebum content, and the soothing effect is represented by the improvement of skin discomfort self-assessment score. The formula is as follows:

[0149] TEWL value improvement rate = (T0 sample group - T28 sample group) / T0 sample group * 100%;

[0150] Skin moisture content improvement rate = (T28 sample group - T0 sample group) / T0 sample group * 100%;

[0151] Sebum improvement rate = (T0 sample group - T28 sample group) / T0 sample group * 100%;

[0152] Skin discomfort improvement rate = (T0 sample group - T28 sample group) / T0 sample group * 100;

[0153] The test results are shown in Table 6 and Figure 1 shown.

[0154] Table 6

[0155]

[0156]

[0157] From the experimental data in Table 6, it can be seen that when the facial cream containing the soothing repairing composition of the present invention is used, the TEWL improvement rate reaches more than 14%, the sebum content improvement rate reaches more than 26%, the moisture content improvement rate reaches more than 37%, and the discomfort improvement rate reaches more than 46%, which indicates that the soothing repairing composition of the present invention has better repairing, moisturizing, oil control and soothing effects.

[0158] From comparative examples 1-5, it can be seen that when the soothing repairing composition comprises the following components in parts by weight: 2-3 parts of hydrolyzed yeast protein, 1.5-2 parts of horse chestnut extract, 1-1.5 parts of marine oligosaccharides, 1.5-2 parts of broom leaf Australian tea extract, and 1-3 parts of medicinal pore fungus extract, the facial cream containing the repairing composition has a TEWL improvement rate of more than 18%, a sebum content improvement rate of more than 41%, a moisture content improvement rate of more than 49%, and a discomfort improvement rate of more than 60%, indicating that the soothing repairing composition of the present invention has better repairing, moisturizing, oil control and soothing effects.

[0159] By comparing Example 1 with Examples 6-12, it can be seen that by using a low eutectic solvent composed of a hydrogen bond acceptor of glycerol glucoside and a hydrogen bond donor of bosine or allantoin to extract the broom leaf Australian tea extract, the facial cream containing the repair composition has a TEWL improvement rate of more than 18%, a sebum content improvement rate of more than 42%, a moisture content improvement rate of more than 50%, and a discomfort improvement rate of more than 62%, indicating that the soothing repair composition of the present invention has better repairing, moisturizing, oil control and soothing effects.

[0160] By comparing Example 1 and Comparative Examples 1-5, it can be seen that by using only one of hydrolyzed yeast protein, horse chestnut extract, marine oligosaccharides, broom leaf Australian tea extract and medicinal pore fungus extract, or using other analogs to replace one of hydrolyzed yeast protein, horse chestnut extract, marine oligosaccharides, broom leaf Australian tea extract and medicinal pore fungus extract, the face cream containing the repair composition has a TEWL improvement rate of less than 13%, a sebum content improvement rate of less than 19%, a moisture content improvement rate of less than 31%, and a discomfort improvement rate of less than 43%; it indicates that there is a synergistic effect between hydrolyzed yeast protein, horse chestnut extract, marine oligosaccharides, broom leaf Australian tea extract and medicinal pore fungus extract, which effectively improves the repair, moisturizing, oil control and soothing effects of the soothing repair composition.

[0161] Finally, it should be noted that the above embodiments are used to illustrate the technical solution of the present disclosure rather than to limit the protection scope of the present disclosure. Although the present disclosure has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present disclosure can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present disclosure.

Claims

1. A soothing and repairing composition for oily and sensitive skin, characterized in that: The invention comprises the following components in parts by weight: 1-5 parts of hydrolyzed yeast protein, 1-3 parts of horse chestnut extract, 0.5-2 parts of marine oligosaccharide, 1-3 parts of broom leaf Australian tea extract and 1-3 parts of medicinal pore fungus extract.

2. The soothing and repairing composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 2-3 parts of hydrolyzed yeast protein, 1.5-2 parts of horse chestnut extract, 1-1.5 parts of marine oligosaccharide, 1.5-2 parts of broom leaf Australian tea extract and 1-3 parts of medicinal pore fungus extract.

3. The soothing and repairing composition according to claim 1, characterized in that: The preparation method of the broom leaf Australian tea leaf extract comprises: adding the broom leaf Australian tea leaf into a low eutectic solvent, extracting at a temperature of 50-80° C. for 2-4 hours, and performing solid-liquid separation to obtain a liquid as the broom leaf Australian tea leaf extract, wherein the hydrogen bond acceptor of the low eutectic solvent is glycerol glucoside, and the hydrogen bond donor is phosphene or allantoin.

4. The soothing and repairing composition according to claim 3, characterized in that: The mass ratio of the hydrogen bond donor to the hydrogen bond acceptor is 1:1-1:

2.

5. The soothing and repairing composition according to claim 3, characterized in that: The mass ratio of the Australian tea leaves to the deep eutectic solvent is 1:5-10.

6. Use of the soothing and repairing composition according to any one of claims 1 to 5 in the preparation of cosmetics.

7. A cosmetic, characterized in that: The cosmetic comprises the soothing and repairing composition according to any one of claims 1 to 5, and the mass percentage of the soothing and repairing composition in the cosmetic is 0.5-10%.

8. The cosmetic according to claim 8, characterized in that The cosmetic is at least one of lotion, emulsion, cream, mask, essence and spray.

9. The cosmetic according to claim 8, characterized in that The cosmetic is a facial cream, and the facial cream comprises the following components in percentage by mass: 0.5-10% of a soothing and repairing composition, 0.05-0.3% of a thickener, 0.5-1% of a moisturizer, 0.5-5% of an emulsifier, 0.5-3% of a preservative, 0.01-0.3% of a pH regulator, and the remainder being water.

10. The cosmetic according to claim 9, characterized in that The thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol; And / or, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, 1,3-propylene glycol, glycerol, budding septin polysaccharide, and ceramide; And / or, the pH regulator includes at least one of arginine, tromethamine, and EDTA-disodium; and / or, the emulsifier comprises at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane / polysiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate; And / or, the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer.

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