Production process for ultrasonic extraction of dihydroquercetin

Abstract

The invention discloses a production process for extracting dihydroquercetin by ultrasonic waves, which comprises the following steps: step 1, freezing: taking larch as a raw material, crushing the larch into 30-50 meshes, performing freezing and ice crystallization in an environment of-8 DEG C to-20 DEG C, and then dispersing the larch in an aqueous solution of 0-4 DEG C to obtain a feed liquid; 2, wall breaking: mechanically grinding the obtained feed liquid, uniformly mixing, and then carrying out instant ultrahigh pressure wall breaking by using a high-pressure homogenizer, so as to obtain a cell wall breaking material; according to the method, raw materials are soaked with 98% ethyl alcohol and then stirred at a high speed, plant cells of larch become soft and thin, and cell walls of larch generate stronger collapse and maximum rupture in a frequency conversion and cosine combined ultrasonic extraction mode, so that the yield of larch is increased, and the yield of larch is increased. The method ensures that the dihydroquercetin can be rapidly fused into an ethanol solution from plant cells, the yield of dihydroquercetin extraction is improved to the maximum extent, and the extraction rate of dihydroquercetin is 90% or above.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of dihydroquercetin, and in particular to a production process for ultrasonic extraction of dihydroquercetin. Background Art

[0002] Dihydroquercetin belongs to flavonoids and is named vitamin P. It is a strong antioxidant that can prevent the activity of active oxygen (free radicals) in the human body, has the effects of anti-radiation, detoxification, and antibody countercurrent, and can inhibit the pathological changes of peracid oxidized lipid compounds and cell membranes. With the aging of society and the improvement of living standards, the incidence and mortality of cardiovascular and cerebrovascular diseases are increasing. Studies in recent years have shown that dihydroquercetin can be used for oxidative stress diseases (cardiovascular disease, lung disease, etc.). In addition, dihydroquercetin can also be used as a food antioxidant. It can be added to vegetable oils, animal oils, dry milk powder, and fat-containing pastries to extend the shelf life. Therefore, the extraction and purification of dihydroquercetin are also attracting more and more attention.

[0003] The efficiency of dihydroquercetin extracted by traditional methods is not very optimistic. There are problems of serious waste of raw materials and high cost. At the same time, since dihydroquercetin exists in larch cells, the extraction of dihydroquercetin generally relies on traditional crushing methods, such as grinding method, which has serious loss of liquid. Moreover, due to mechanical heat generation, even with cooling equipment, there will still be a large loss of active ingredients in larch cells, and it will also destroy the biological macromolecules in the cells, resulting in a very low extraction rate. Summary of the invention

[0004] In view of the defects of the prior art, the purpose of the present invention is to provide a production process for ultrasonic extraction of dihydroquercetin to solve the problem of serious waste of raw materials in the existing traditional crushing method for extracting dihydroquercetin proposed in the above background technology. Since dihydroquercetin exists in larch cells, the heat generated during mechanical crushing will cause a large loss of active ingredients in the larch cells and will also destroy the biological macromolecules in the cells, resulting in an extremely low extraction rate.

[0005] The present invention solves the technical problem by adopting the following technical solution:

[0006] The present invention provides a production process for ultrasonic extraction of dihydroquercetin, comprising the following steps:

[0007] Step 1, freezing: using larch as raw material, crushing it into 30-50 meshes, then freezing and ice crystallizing it in an environment of -8°C to -20°C, and then dispersing it in an aqueous solution at 0°C to 4°C to obtain a feed liquid;

[0008] Step 2, cell wall breaking: the obtained liquid material is mechanically ground and mixed evenly, and then a high-pressure homogenizer is used to instantly break the cell wall to obtain a cell wall breaking material;

[0009] Step 3, ultrasonic extraction: add the cell wall material into 98% ethanol solution, soak for 2-3 hours, and stir at high speed to form a homogenate, use variable frequency ultrasound and cosine ultrasound alternately, extract under ultrasonic vibration conditions to obtain an extract;

[0010] Step 4: hydrolysis adsorption: add hydrochloric acid to the extract, heat and reflux for 2-3 hours, adsorb on a macroporous resin column and collect the eluate;

[0011] Step 5, concentration: distilling the eluate to obtain a concentrate, adding 98% ethanol to the concentrate for crystallization to obtain a crude dihydroquercetin product;

[0012] Step 6: The obtained crude dihydroquercetin is crystallized multiple times to obtain dihydroquercetin.

[0013] Preferably, the ultrasonic extraction in step three is assisted by stirring, the stirring temperature is 40-50° C., the stirring speed is 900-1000 r / min, and the stirring time is not less than 50 min.

[0014] Preferably, in the step three, the ultrasonic extraction is performed by using variable frequency ultrasonic treatment for 5 minutes and then using cosine ultrasonic treatment for 7 minutes, alternating for 3-4 times.

[0015] Preferably, in the step three ultrasonic extraction, the power of the ultrasonic wave is 90w-150w, and the ultrasonic wave frequency is 15-30KHz.

[0016] Preferably, in the ultrasonic extraction in step three, the power of the ultrasonic wave is 100w, and the ultrasonic wave frequency is 20KHz.

[0017] Preferably, the crude dihydroquercetin in step six is ​​first placed in liquid carbon dioxide before recrystallization, and carbon dioxide is circulated at an extraction temperature of 8-25° C. to extract and separate the crude dihydroquercetin.

[0018] Preferably, the moisture content of the larch is 15-20%, and the aqueous solution is 2-3 times of the total mass.

[0019] Preferably, the aqueous solution is a mixed solution of edible alcohol and water, and the mass ratio of the edible alcohol to water is 2:8-4:6.

[0020] Preferably, the mechanical milling is one of a grinding wheel mill, a colloid mill or an oscillating mill, and the milling mixing time is 40-60 minutes.

[0021] Preferably, when the high-pressure homogenizer performs instantaneous ultra-high pressure wall breaking, the wall breaking pressure is 80 MPa-120 MPa, and the number of wall breaking times is 2-3 times.

[0022] The ultrasonic extraction dihydroquercetin production process of the present invention comprises the following steps: freezing larch plant cells to make the cells ice-crystallized. The low temperature at this time causes the water in the larch plant cells to freeze, forming reverse expansion, and making the cell walls brittle (the lower the freezing temperature, the better the effect, and the more brittle the cell walls). Then, the villi on the surface of the cell walls are removed by mechanical grinding and mixing, so that the cell walls are completely exposed. Finally, ultrasonic technology and an ultra-high pressure homogenizer are used to break the ice-crystallized larch plant cells into nano-scale particles, thereby better completing the cell wall breaking and improving the output rate of dihydroquercetin.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The ultrasonic extraction production process of dihydroquercetin of the present invention comprises the following steps: soaking ground larch raw materials in 98% ethanol, and then stirring at a high speed to soften and thin the plant cells of the larch; and combining the process with an ultrasonic extraction method combining frequency conversion and cosine, which not only increases the vibration amplitude and the mass transfer surface area, but also reduces the dead angle caused by standing waves, so that the cell wall of the larch is more strongly collapsed and ruptured to the maximum extent, ensuring that the dihydroquercetin can be quickly integrated into the ethanol solution from the plant cells, maximally improving the yield of the dihydroquercetin extraction, and making the extraction rate of the dihydroquercetin above 90%. DETAILED DESCRIPTION

[0025] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] The present invention of this embodiment provides a production process for ultrasonic extraction of dihydroquercetin, comprising the following steps:

[0027] Step 1, freezing: using larch as raw material, crushing it into 30-50 meshes, then freezing and ice crystallizing it in an environment of -8°C to -20°C, and then dispersing it in an aqueous solution at 0°C to 4°C to obtain a feed liquid;

[0028] Step 2, cell wall breaking: the obtained liquid material is mechanically ground and mixed evenly, and then a high-pressure homogenizer is used to instantly break the cell wall to obtain a cell wall breaking material;

[0029] Step 3, ultrasonic extraction: add the cell wall material into 98% ethanol solution, soak for 2-3 hours, and stir at high speed to form a homogenate, use variable frequency ultrasound and cosine ultrasound alternately, extract under ultrasonic vibration conditions to obtain an extract;

[0030] Step 4: hydrolysis adsorption: add hydrochloric acid to the extract, heat and reflux for 2-3 hours, adsorb on a macroporous resin column and collect the eluate;

[0031] Step 5, concentration: distilling the eluate to obtain a concentrate, adding 98% ethanol to the concentrate for crystallization to obtain a crude dihydroquercetin product;

[0032] Step 6: The obtained crude dihydroquercetin is crystallized multiple times to obtain dihydroquercetin.

[0033] In step 3 of this embodiment, the ultrasonic extraction is assisted by stirring, the stirring temperature is 40-50° C., the stirring speed is 900-1000 r / min, and the stirring time is not less than 50 min.

[0034] In step 3 of the present embodiment, during ultrasonic extraction, variable frequency ultrasonic treatment is performed for 5 minutes, followed by cosine ultrasonic treatment for 7 minutes, and the process is repeated 3-4 times.

[0035] In step 3 of the present embodiment, the power of the ultrasonic wave in the ultrasonic extraction is 90w-150w, and the ultrasonic wave frequency is 15-30KHz.

[0036] In step 3 of the present embodiment, the power of the ultrasonic wave in the ultrasonic extraction is 100W, and the ultrasonic wave frequency is 20KHz.

[0037] Before recrystallization, the crude dihydroquercetin in step 6 of this embodiment is first placed in liquid carbon dioxide, and carbon dioxide is circulated at an extraction temperature of 8-25° C. to extract and separate the crude dihydroquercetin.

[0038] The moisture content of the larch in this embodiment is 15-20%, and the aqueous solution is 2-3 times of the total mass.

[0039] The aqueous solution of the present embodiment is a mixed solution of edible alcohol and water, and the mass ratio of edible alcohol to water is 2:8-4:6.

[0040] The mechanical grinding in this embodiment is one of grinding wheel grinding, colloid grinding or oscillating grinding, and the grinding and mixing time is 40-60 minutes.

[0041] When the high-pressure homogenizer of this embodiment performs instantaneous ultra-high pressure wall breaking, the wall breaking pressure is 80 MPa-120 MPa, and the number of wall breaking times is 2-3 times.

[0042] Example 1.

[0043] The present invention of this embodiment provides a production process for ultrasonic extraction of dihydroquercetin, comprising the following steps:

[0044] Step 1, freezing: using larch as raw material, crushing it into 30 meshes, then freezing and ice crystallizing it at -8°C, and then dispersing it in a 0°C aqueous solution to obtain a feed liquid;

[0045] Step 2, cell wall breaking: the obtained liquid material is mechanically ground and mixed evenly, and then a high-pressure homogenizer is used to instantly break the cell wall to obtain a cell wall breaking material;

[0046] Step 3, ultrasonic extraction: add the cell wall material into 98% ethanol solution, soak for 2 hours, and stir at high speed to form a homogenate, use variable frequency ultrasound and cosine ultrasound alternately, extract under ultrasonic vibration conditions to obtain an extract;

[0047] Step 4: hydrolysis adsorption: add hydrochloric acid to the extract, heat and reflux for 2 hours, adsorb on a macroporous resin column and collect the eluate;

[0048] Step 5, concentration: distilling the eluate to obtain a concentrate, adding 98% ethanol to the concentrate for crystallization to obtain a crude dihydroquercetin product;

[0049] Step 6: The obtained crude dihydroquercetin is crystallized multiple times to obtain dihydroquercetin.

[0050] In step 3 of this embodiment, the ultrasonic extraction is assisted by stirring, the stirring temperature is 40° C., the stirring speed is 900 r / min, and the stirring time is not less than 50 min.

[0051] In step 3 of the present embodiment, ultrasonic extraction is performed by using variable frequency ultrasonic treatment for 5 minutes, and then using cosine ultrasonic treatment for 7 minutes, alternating for 3 times.

[0052] In step 3 of the present embodiment, the power of the ultrasonic wave in the ultrasonic extraction is 90W, and the ultrasonic wave frequency is 15KHz.

[0053] Before recrystallization, the crude dihydroquercetin in step 6 of this embodiment is first placed in liquid carbon dioxide, and carbon dioxide is circulated at an extraction temperature of 8° C. to extract and separate the crude dihydroquercetin.

[0054] The moisture content of larch in this embodiment is 15%, and the aqueous solution is twice the total mass.

[0055] The aqueous solution in this embodiment is a mixed solution of edible alcohol and water, and the mass ratio of edible alcohol to water is 2:8.

[0056] The mechanical grinding in this embodiment is one of grinding wheel grinding, colloid grinding or oscillating grinding, and the grinding and mixing time is 40 minutes.

[0057] When the high-pressure homogenizer of this embodiment performs instantaneous ultra-high pressure wall breaking, the wall breaking pressure is 80mpampa and the number of wall breaking times is 2 times.

[0058] Example 2.

[0059] The present invention of this embodiment provides a production process for ultrasonic extraction of dihydroquercetin, comprising the following steps:

[0060] Step 1, freezing: using larch as raw material, crushing it into 40 meshes, then freezing and ice crystallizing it at -15°C, and then dispersing it in a 2°C aqueous solution to obtain a feed liquid;

[0061] Step 2, cell wall breaking: the obtained liquid material is mechanically ground and mixed evenly, and then a high-pressure homogenizer is used to instantly break the cell wall to obtain a cell wall breaking material;

[0062] Step 3, ultrasonic extraction: add the cell wall-breaking material into 98% ethanol solution, soak for 2.5 hours, and stir at high speed to form a homogenate, use variable frequency ultrasound and cosine ultrasound alternately, extract under ultrasonic vibration conditions to obtain an extract;

[0063] Step 4: hydrolysis adsorption: add hydrochloric acid to the extract, heat and reflux for 2.5 hours, adsorb on a macroporous resin column and collect the eluate;

[0064] Step 5, concentration: distilling the eluate to obtain a concentrate, adding 98% ethanol to the concentrate for crystallization to obtain a crude dihydroquercetin product;

[0065] Step 6: The obtained crude dihydroquercetin is crystallized multiple times to obtain dihydroquercetin.

[0066] In step 3 of this embodiment, the ultrasonic extraction is assisted by stirring, the stirring temperature is 45° C., the stirring speed is 950 r / min, and the stirring time is not less than 50 min.

[0067] In step 3 of the present embodiment, during ultrasonic extraction, variable frequency ultrasonic treatment is performed for 5 minutes, followed by cosine ultrasonic treatment for 7 minutes, and the process is repeated three times alternately.

[0068] In step 3 of the present embodiment, the power of the ultrasonic wave in the ultrasonic extraction is 100W, and the ultrasonic wave frequency is 20KHz.

[0069] Before recrystallization, the crude dihydroquercetin in step 6 of this embodiment is first placed in liquid carbon dioxide, and carbon dioxide is circulated at an extraction temperature of 20° C. to extract and separate the crude dihydroquercetin.

[0070] The moisture content of the larch in this embodiment is 18%, and the aqueous solution is 2.5 times of the total mass.

[0071] The aqueous solution in this embodiment is a mixed solution of edible alcohol and water, and the mass ratio of edible alcohol to water is 5:5.

[0072] The mechanical grinding in this embodiment is one of grinding wheel grinding, colloid grinding or oscillating grinding, and the grinding and mixing time is 50 minutes.

[0073] When the high-pressure homogenizer of this embodiment performs instantaneous ultra-high pressure wall breaking, the wall breaking pressure is 100 MPa and the number of wall breaking times is 3 times.

[0074] Example 3.

[0075] The present invention of this embodiment provides a production process for ultrasonic extraction of dihydroquercetin, comprising the following steps:

[0076] Step 1, freezing: using larch as a raw material, crushing it into 50 meshes, then freezing and ice crystallizing it at -20°C, and then dispersing it in a 4°C aqueous solution to obtain a feed liquid;

[0077] Step 2, cell wall breaking: the obtained liquid material is mechanically ground and mixed evenly, and then a high-pressure homogenizer is used to instantly break the cell wall to obtain a cell wall breaking material;

[0078] Step 3, ultrasonic extraction: add the cell wall material into 98% ethanol solution, soak for 3 hours, and stir at high speed to form a homogenate, use variable frequency ultrasound and cosine ultrasound alternately, extract under ultrasonic vibration conditions to obtain an extract;

[0079] Step 4: hydrolysis adsorption: add hydrochloric acid to the extract, heat and reflux for 3 hours, adsorb on a macroporous resin column and collect the eluate;

[0080] Step 5, concentration: distilling the eluate to obtain a concentrate, adding 98% ethanol to the concentrate for crystallization to obtain a crude dihydroquercetin product;

[0081] Step 6: The obtained crude dihydroquercetin is crystallized multiple times to obtain dihydroquercetin.

[0082] In step 3 of this embodiment, the ultrasonic extraction is assisted by stirring, the stirring temperature is 50° C., the stirring speed is 1000 r / min, and the stirring time is 100 min.

[0083] In step 3 of the present embodiment, during ultrasonic extraction, variable frequency ultrasonic treatment is performed for 5 minutes, followed by cosine ultrasonic treatment for 7 minutes, and the process is repeated four times alternately.

[0084] In step 3 of the present embodiment, the power of the ultrasonic wave in the ultrasonic extraction is 150W, and the ultrasonic wave frequency is 30KHz.

[0085] Before recrystallization, the crude dihydroquercetin in step 6 of this embodiment is first placed in liquid carbon dioxide, and carbon dioxide is circulated at an extraction temperature of 25° C. to extract and separate the crude dihydroquercetin.

[0086] The moisture content of larch in this embodiment is 20%, and the aqueous solution is 3 times of the total mass.

[0087] The aqueous solution in this embodiment is a mixed solution of edible alcohol and water, and the mass ratio of edible alcohol to water is 4:6.

[0088] The mechanical grinding in this embodiment is one of grinding wheel grinding, colloid grinding or oscillating grinding, and the grinding and mixing time is 60 minutes.

[0089] When the high-pressure homogenizer of this embodiment performs instantaneous ultra-high pressure wall breaking, the wall breaking pressure is 120 MPa and the number of wall breaking times is 3 times.

[0090] Comparative Example 1.

[0091] The difference from Example 3 is that a common method is used to extract dihydroquercetin.

[0092] Comparative Example 2.

[0093] The difference from Example 3 is that the freezing and crushing method is not used to extract dihydroquercetin.

[0094] Comparative Example 3.

[0095] The difference from Example 3 is that the alternating method of variable frequency ultrasound and cosine ultrasound is not used to extract dihydroquercetin.

[0096] The products of Examples 1-3 and Comparative Examples 1-3 were subjected to performance tests:

[0097] Dihydroquercetin was extracted using larch as raw material and divided into 6 groups, each group corresponding to Examples 1-3 and Comparative Examples 1-3 and numbered A / B / C / D / E / F. Finally, the dihydroquercetin in groups A / B / C / D / E / F was tested, and the test results are shown in the following table.

[0098] Extraction rate % Pure rate A 96％ 96％ B 94％ 96％ C 93％ 95％ D 80％ 91％ E 85％ 92％ F 85％ 92％

[0099] It can be seen from Examples 1-3 that the cell wall can be completely exposed by the freezing and crushing method, and the ground larch raw material is soaked in 98% ethanol to soften and thin the larch plant cells, and combined with the ultrasonic extraction method combined with variable frequency plus cosine, the yield of dihydroquercetin extraction is maximized, so that the extraction rate of dihydroquercetin is above 90%.

[0100] It can be seen from Examples 1-3 and Comparative Example 3 that the crushed larch raw material is soaked in 98% ethanol and then stirred at high speed to soften and thin the larch plant cells, and combined with the ultrasonic extraction method combined with variable frequency plus cosine, not only the vibration amplitude and mass transfer surface area are increased, but also the dead angle caused by standing waves is reduced, so that the larch cell wall collapses more strongly and breaks to the maximum extent, ensuring that dihydroquercetin can be quickly integrated into the ethanol solution from the plant cells, thereby maximizing the yield of dihydroquercetin extraction.

[0101] It can be seen from Comparative Examples 1-3 that the traditional mechanical cell wall breaking destroys the active ingredients of dihydroquercetin and it is difficult to break the larch plant cells into nano-scale particles, resulting in a low extraction rate of dihydroquercetin. The extraction rate of ultrasonic extraction alone is also poor. Therefore, while ensuring the purity rate, the extraction rate of the extraction process of the present invention is much higher than that of the existing extraction method.

[0102] The innovation of the present invention is:

[0103] The invention freezes the plant cells of larch to make the cells ice-crystallized. The low temperature at this time will freeze the water in the plant cells of larch to form reverse expansion, so that the cell wall is brittle (the lower the freezing temperature, the better the effect and the brittler the cell wall). Then, the villi on the surface of the cell wall are removed by mechanical grinding and mixing to make the cell wall completely exposed. Finally, ultrasonic technology and ultra-high pressure homogenizer are used to break the ice-crystallized larch plant cells into nano-scale particles, so as to better complete the cell wall breaking and improve the output rate of dihydroquercetin. The crushed pine raw materials are soaked in 98% ethanol and then stirred at high speed to soften and thin the larch plant cells. Combined with the ultrasonic extraction method of variable frequency plus cosine, it not only increases the vibration amplitude and mass transfer surface area, but also reduces the dead corners caused by standing waves, causing the larch cell wall to collapse more strongly and rupture to the maximum extent, ensuring that dihydroquercetin can be quickly integrated into the ethanol solution from the plant cells, maximizing the extraction yield of dihydroquercetin, and making the extraction rate of dihydroquercetin above 90%.

[0104] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0105] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A production process for extracting dihydroquercetin by ultrasonic wave, characterized in that: The following steps are involved: Step 1, freezing: using larch as raw material, crushing it into 30-50 meshes, then freezing and ice crystallizing it in an environment of -8°C to -20°C, and then dispersing it in an aqueous solution at 0°C to 4°C to obtain a feed liquid; Step 2, cell wall breaking: the obtained liquid material is mechanically ground and mixed evenly, and then a high-pressure homogenizer is used to instantly break the cell wall to obtain a cell wall breaking material; Step 3, ultrasonic extraction: add the cell wall material into 98% ethanol solution, soak for 2-3 hours, and stir at high speed to form a homogenate, use variable frequency ultrasound and cosine ultrasound alternately, extract under ultrasonic vibration conditions to obtain an extract; Step 4: hydrolysis adsorption: add hydrochloric acid to the extract, heat and reflux for 2-3 hours, adsorb on a macroporous resin column and collect the eluate; Step 5, concentration: distilling the eluate to obtain a concentrate, adding 98% ethanol to the concentrate for crystallization to obtain a crude dihydroquercetin product; Step 6: The obtained crude dihydroquercetin is crystallized multiple times to obtain dihydroquercetin.

2. The production process for ultrasonic extraction of dihydroquercetin according to claim 1, characterized in that: The ultrasonic extraction in step 3 is assisted by stirring, the stirring temperature is 40-50° C., the stirring speed is 900-1000 r / min, and the stirring time is not less than 50 min.

3. The production process of ultrasonic extraction of dihydroquercetin according to claim 2, characterized in that: In the step 3, during the ultrasonic extraction, variable frequency ultrasonic treatment is performed for 5 minutes, and then cosine ultrasonic treatment is performed for 7 minutes, and the process is repeated 3-4 times.

4. The production process for ultrasonic extraction of dihydroquercetin according to claim 3, characterized in that: In the step 3, the power of the ultrasonic wave in the ultrasonic extraction is 90w-150w, and the ultrasonic wave frequency is 15-30KHz.

5. The production process of ultrasonic extraction of dihydroquercetin according to claim 4, characterized in that: The power of the ultrasonic wave in the ultrasonic extraction of step 3 is 100W, and the ultrasonic wave frequency is 20KHz.

6. A production process for ultrasonic extraction of dihydroquercetin according to any one of claims 1 to 5, characterized in that: The crude dihydroquercetin in step six is ​​first placed in liquid carbon dioxide before recrystallization, and carbon dioxide is circulated at an extraction temperature of 8-25° C. to extract and separate the crude dihydroquercetin.

7. The production process of ultrasonic extraction of dihydroquercetin according to claim 1, characterized in that: The moisture content of the larch is 15-20%, and the aqueous solution is 2-3 times of the total mass.

8. The production process for ultrasonic extraction of dihydroquercetin according to claim 7, characterized in that: The aqueous solution is a mixed solution of edible alcohol and water, and the mass ratio of the edible alcohol to water is 2:8-4:

6.

9. The production process for ultrasonic extraction of dihydroquercetin according to claim 8, characterized in that: The mechanical milling is one of a grinding wheel mill, a colloid mill or an oscillating mill, and the milling mixing time is 40-60 minutes.

10. The production process of ultrasonic extraction of dihydroquercetin according to claim 9, characterized in that: When the high-pressure homogenizer performs instantaneous ultra-high pressure wall breaking, the wall breaking pressure is 80 MPa-120 MPa, and the number of wall breaking times is 2-3 times.