Composition with blood sugar reducing function and preparation method thereof

Abstract

The invention belongs to the technical field of hypoglycemic health-care products, and particularly relates to a composition with a hypoglycemic function and a preparation method thereof. The composition with the function of reducing blood sugar comprises the following components in parts by weight: 5-20 parts of an oriental cherry extract, 128-175 parts of a cinnamon extract, 1-5 parts of a bitter gourd extract, 60-90 parts of a mulberry leaf extract and 5-20 parts of chromium-enriched yeast. The composition with the hypoglycemic function can be used for preparing hypoglycemic related functional food or health care products, the components of the composition formula are basically extracted from natural products, the raw materials or raw medicinal materials are basically edible food, and the composition has the advantages of being convenient and easy to obtain, free of toxic and side effects, safe and reliable. Through alpha-glucosidase inhibition rate and alpha-amylase inhibition rate model experiments, the effect of the composition with the blood sugar reducing function on the aspect of reducing blood sugar is verified, and the blood sugar control effect of the composition is superior to that of positive control acarbose.

Description

Technical Field

[0001] The invention belongs to the technical field of blood sugar lowering health care products, and specifically relates to a blood sugar lowering composition and a preparation method thereof. Background Art

[0002] Diabetes is a metabolic disorder syndrome associated with abnormal insulin production and action in the body, especially with hyperglycemia as its main feature. Its triggering factors may be genetics, immune dysfunction, microbial infection, free radical toxins, diet and lifestyle habits.

[0003] Diabetes is divided into type Ⅰ and type Ⅱ diabetes. Type Ⅰ diabetes is related to genetics and has a low incidence rate. Type Ⅱ diabetes is the main type of diabetes patients at present, which is manifested by insulin resistance, impaired pancreatic β-cell function and chronic inflammatory response caused by oxidative stress. Currently, Western medicine is mainly used for the treatment of diabetes, such as insulin injection; insulin secretagogues sulfonylureas and glinides; biguanides; α-glucosidase inhibitor acarbose; insulin sensitizers such as pioglitazone, etc. Although the above-mentioned Western medicines have good targeting effects and can quickly and efficiently lower blood sugar, they are long-term dependent and their liver and kidney toxicity cannot be underestimated. For example, sulfonylurea drugs may cause leukopenia; biguanide hypoglycemic drugs are more harmful to the stomach and intestines; α-glucosidase inhibitors will produce gastrointestinal reactions, and the side effects are more obvious when taking large doses or taking them for a long time, and long-term use will cause drug resistance, affecting the efficacy.

[0004] Diabetes is a chronic disease with a long and complex course, so it is difficult to cure. It is called "Xiaoke" in traditional Chinese medicine. Traditional Chinese medicine has a certain effect on the treatment of diabetes and has the characteristics of mild medication, long duration and few side effects. Modern research shows that the active ingredients contained in some Chinese herbal medicines have the effect of lowering blood sugar, among which polysaccharides and polypeptides are the most. In recent years, the resources of "medicine and food are of the same origin" have been continuously expanded. Among them, some Chinese medicinal materials, new food raw materials and compound preparations composed of them play an important role in the prevention and treatment of diabetes. Therefore, the research and development of Chinese herbal medicines for the treatment of diabetes has become the key to the development of new drugs and is of great significance.

[0005] In the prior art, patent CN 108066437 B uses mulberry leaves, emblica, hawthorn, purslane, cinnamon, and bitter melon to form a Chinese medicine composition, and all raw materials are extracted with simple water to obtain the product. The simple water extract contains a large amount of non-functional impurities and a high yield of finished products, resulting in a large daily dosage of the finished product. Moreover, the product is in the form of tablets, which requires the addition of a large amount of preparation excipients such as compressible starch and maltodextrin, which is an additional burden for diabetics. Patent CN103463207 A uses cinnamon, psyllium, mulberry leaves, and bitter melon as a formula combination for the prevention and treatment of spleen and kidney yang deficiency type diabetes. The patent screens the formula for diabetes from the perspective of diabetes in traditional Chinese medicine, and defines the standards for four raw materials: cinnamon polyphenols ≥ 10%, psyllium polysaccharides ≥ 10%, mulberry leaf DNJ content ≥ 1%, and bitter melon total saponins ≥ 10%. CN102727586 A patent invented a composition for preventing and treating diabetes, the formula includes three ingredients: cinnamon, mulberry leaves, and bitter melon, and the distribution is mainly used for spleen and kidney yang deficiency type diabetes. CN 117919305 A patent developed a Chinese medicine hypoglycemic formula made of astragalus, bitter melon, mulberry leaves, cornus officinalis, kudzu root, ginseng, and chromium-rich yeast. The raw materials are extracted and concentrated by supercritical wall-breaking extraction technology, and the finished products have three dosage forms: capsules, granules, and tablets. Although its product formula includes bitter melon, mulberry leaves, and chromium-rich yeast, it does not clearly define the functional ingredients for hypoglycemic bitter melon polypeptides in bitter melon and 1-deoxynojirimycin in mulberry leaves. The product dosage is converted according to the medicinal materials, and the actual effect will be affected by the quality of different batches of medicinal materials and cannot be quantified. CN104383259 A patent invented a product formula that can improve the fasting and postprandial blood sugar lowering function of the human body, including bitter melon, buckwheat, mulberry leaves, cornus officinalis, polygonatum, chromium-rich yeast, and starch. The raw materials used in this patent are all corresponding extracts, but there is no specific product information such as raw material specifications, and the quality stability of the raw materials cannot be guaranteed. Summary of the invention

[0006] The purpose of the present invention is to provide a composition with hypoglycemic function and a preparation method thereof. The composition with hypoglycemic function of the present invention can be used for daily use by diabetic patients to achieve the effect of lowering blood sugar and can reduce the toxic side effects of long-term use of chemical drugs on the human body.

[0007] The implementation process of the present invention is as follows:

[0008] A composition with hypoglycemic function, comprising the following components in parts by weight:

[0009] 5 to 20 parts by weight of cherry blossom extract,

[0010] 128-175 parts by weight of cinnamon extract,

[0011] 1 to 5 parts by weight of bitter melon extract,

[0012] 60-90 parts by weight of mulberry leaf extract,

[0013] 5 to 20 parts by weight of chromium-enriched yeast.

[0014] The above-mentioned composition with hypoglycemic function preferably comprises the following components in parts by weight: 10 parts by weight of cherry blossom extract, 150 parts by weight of cinnamon extract, 5 parts by weight of bitter melon extract, 75 parts by weight of mulberry leaf extract, and 10 parts by weight of chromium-enriched yeast.

[0015] The preparation method of the above-mentioned composition having the function of lowering blood sugar comprises the following steps:

[0016] (1) Weigh 5 to 20 parts by weight of cherry blossom extract, 128 to 175 parts by weight of cinnamon extract, 1 to 5 parts by weight of bitter melon extract, 60 to 90 parts by weight of mulberry leaf extract, and 5 to 20 parts by weight of chromium-enriched yeast respectively, and then put them into a mixer for mixing;

[0017] (2) The mixed materials are crushed by a crusher, and the crushed materials are sieved through a sieve;

[0018] (3) The sieved materials are mixed for a second time using a mixer, the materials obtained after the second mixing are collected, and the materials are dry-pressed and granulated using a granulator, and the granules obtained by dry-pressing and granulating are collected and packaged to obtain the product.

[0019] Furthermore, the mixing time in step (1) is 40 to 50 minutes; the crushing time in step (2) is 5 to 10 minutes; the mesh size of the sieve is 60 to 70 meshes; the secondary mixing time in step (3) is 40 to 50 minutes, and the dry pressing pressure is 8 to 12 kN / cm.

[0020] Furthermore, the preparation process of the cherry blossom extract is that after the cherry blossoms are picked, they are soaked and extracted in 45% to 55% ethanol by mass at a temperature of 55 to 65°C for 2 to 3 hours, the extract is initially filtered using a ceramic membrane and then separated and concentrated using a nanofiltration membrane, the concentrated solution is collected and diluted with water, the diluted solution is adsorbed using a macroporous resin, washed with water, and analyzed with 75% to 85% ethanol by mass, the analyzed solution is collected, replenished with water, and concentrated, and then spray-dried and collected to obtain a dry powdered cherry blossom extract, wherein the total flavonoid content of cherry blossoms is ≥30%.

[0021] Furthermore, the parameters of the ceramic membrane are a ceramic membrane with a pore size of 8 to 10 μm, the parameters of the nanofiltration membrane are a nanofiltration membrane with a pore size of 1 to 4 nm, the parameters of the macroporous resin are D101 macroporous resin, the parameters of the spray drying are an inlet air temperature of 180 to 220°C, an outlet air temperature of 100 to 140°C, and the mesh size of the sieving screen is 60 to 70 meshes.

[0022] Furthermore, the preparation process of the cinnamon extract is as follows: the cinnamon medicinal material is coarsely crushed, and then refluxed and extracted 1 to 5 times with 75% to 85% ethanol by mass, the extract is collected and filtered, the filtrate is collected and concentrated after adding water to obtain a concentrated solution, after instantaneous high-temperature sterilization, the concentrated solution is spray-dried, the obtained spray-dried powder is collected, and after crushing and sieving, a dry powder cinnamon extract is obtained, wherein the cinnamon polyphenol content is ≥30%.

[0023] Furthermore, the temperature of the instantaneous high temperature sterilization is 120-150°C, the parameters of the spray drying are an inlet air temperature of 180-220°C, an outlet air temperature of 100-140°C, and the mesh number of the sieving screen is 60-70 meshes.

[0024] Furthermore, the preparation process of the bitter melon extract is as follows: the bitter melon is chopped, water is added for reflux extraction 1 to 5 times, the extract solution is filtered, the filtrate is cooled to room temperature and then enzymatically hydrolyzed with a protease, the enzymatically hydrolyzed liquid is boiled and inactivated, the enzymatically hydrolyzed liquid is concentrated, and then sterilized at high temperature for an instant, and then the sterilized liquid is spray-dried, the spray-dried powder is collected, and after crushing and sieving, a dry powder bitter melon extract is obtained, wherein the bitter melon polypeptide content is ≥60%.

[0025] Furthermore, the specific process and parameters involved in the protease hydrolysis are adding 0.5% by mass of protease to the extract cooled to 30-40°C, maintaining the temperature at 45-55°C and stirring the hydrolysis for 20-40 minutes, the temperature of the instantaneous high temperature sterilization is 120-150°C, the parameters of the spray drying are an inlet air temperature of 180-220°C, an outlet air temperature of 100-140°C, and the mesh size of the sieving screen is 60-70 meshes.

[0026] The composition with hypoglycemic function of the present invention has been verified by in vitro α-glucosidase inhibition rate and α-amylase inhibition rate experiments. The hypoglycemic effect of the product is better than that of the standard substance acarbose at the same concentration. The blood sugar lowering function of the composition of the present invention is the result of the comprehensive action of multiple pathways, and its main mechanism of action is related to repairing islet cells, promoting islet cells to secrete insulin, and improving the transport capacity of glucose transporters. In the formula ratio of the composition with hypoglycemic function of the present invention, cinnamon extract is used in the largest amount, accounting for up to about 60%. The main mechanism of action of its functional ingredient cinnamon polyphenol is to protect islet cells and stimulate them to secrete insulin, thereby increasing the insulin content in serum and promoting the metabolism of glucose in food digested and absorbed by the gastrointestinal tract in the body to achieve the purpose of lowering blood sugar; mulberry leaf extract and bitter melon extract together account for about 32% of the formula, and their functional ingredients are "1-deoxynojirimycin" and "bitter melon polypeptide". These two ingredients have the function of repairing damaged islet cells and promoting islet cells to secrete insulin. Its function complements cinnamon polyphenols, repairing and protecting islet cells, and restoring the function of human body's own islet cells from the source. It also improves insulin resistance, allowing cinnamon polyphenols to promote insulin secretion from pancreatic cells to fully exert its blood sugar regulation function and improve glucose metabolism; chromium-rich yeast accounts for about 4% of this formula, but the organic chromium in this raw material is an important factor in activating the activity of pancreatic cells, which can promote the secretion of insulin by pancreatic cells and increase the activity of hexokinase and malate dehydrogenase, thereby inhibiting the conversion of sugar into fat and direct metabolism out of the body. Its function is to extend the secretion of insulin by pancreatic cells repaired and protected by cinnamon extract, mulberry leaf extract and bitter melon extract; cherry blossom extract accounts for about 4% of the product formula, and cherry blossom total flavonoids have good anti-inflammatory and oxidative stress damage relief functions. Diabetic patients are usually accompanied by diabetic complications, among which inflammatory response is the most common one. The introduction of cherry blossom total flavonoids in the product makes up for this deficiency, not only solving the blood sugar problem from the root, but also improving complications such as inflammation, reflecting the synergistic combination of the formula, which is far superior to other similar products that only control blood sugar.

[0027] Positive effects of the present invention:

[0028] (1) The blood sugar lowering composition of the present invention is based on the traditional Chinese medicine treatment concept for diabetes (Xiaoke) and combined with modern research on the pathogenesis of diabetes, and is designed based on multi-mechanism and multi-target combination to achieve the blood sugar lowering effect. The composition formula of the present invention achieves both symptomatic and root treatment, has the effects of reducing the body's absorption of sugar in food, improving blood sugar conversion, promoting glycogen synthesis, and improving other physiological inflammation problems of diabetic patients. It can effectively deal with the daily blood sugar problems of type 2 diabetes patients, control the rise of blood sugar after meals, and can directly replace chemical drugs for the treatment of diabetes.

[0029] (2) The composition with hypoglycemic function of the present invention can be used to prepare functional foods or health products related to hypoglycemia. The ingredients of the composition are basically extracted from natural products, and the raw materials or original medicinal materials are basically edible foods. It is convenient, easy to obtain, non-toxic, safe and reliable. Among them, mulberry leaf extract has passed the national new edible raw material evaluation and can be eaten as ordinary food; cinnamon and bitter melon are daily edible vegetables and spices; the source of cherry blossoms is a new resource food Guanshan cherry blossoms, which are developed through proprietary processes, and the product is guaranteed in terms of safety.

[0030] (3) The present invention verifies the efficacy of the composition in lowering blood sugar through α-glucosidase inhibition rate and α-amylase inhibition rate model experiments, and its blood sugar control effect is better than the positive control acarbose. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is the experimental result graph of α-glucosidase inhibition rate of samples 1-10;

[0032] Figure 2 This is a graph showing the experimental results of α-amylase inhibition rate for samples 1-10. DETAILED DESCRIPTION

[0033] The present invention will be further described below with reference to the embodiments.

[0034] The composition with hypoglycemic function of the present invention was subjected to an in vitro hypoglycemic activity experiment to examine the effectiveness and safety of its hypoglycemic function. The basic method currently used for in vitro hypoglycemic effect research is to examine the ability to inhibit the activity of key diabetes enzymes (α-glucosidase, α-amylase). Through the α-glucosidase inhibition rate and α-amylase inhibition rate experiments of the composition with hypoglycemic function of the present invention, it is proved that the composition of the present invention has good in vitro hypoglycemic activity, and the inhibition rate of α-glucosidase and α-amylase are better than the positive control acarbose, and the inhibition rate of α-glucosidase and α-amylase are higher than that of a single raw material or a combination of 2 to 4 raw materials.

[0035] A composition with hypoglycemic function comprises the following components in parts by weight: 5-20 parts by weight of cherry blossom extract, 128-175 parts by weight of cinnamon extract, 1-5 parts by weight of bitter melon extract, 60-90 parts by weight of mulberry leaf extract and 5-20 parts by weight of chromium-enriched yeast.

[0036] The preparation method of the above-mentioned composition having the function of lowering blood sugar comprises the following steps:

[0037] (1) Weigh 5 to 20 parts by weight of cherry blossom extract, 128 to 175 parts by weight of cinnamon extract, 1 to 5 parts by weight of bitter melon extract, 60 to 90 parts by weight of mulberry leaf extract, and 5 to 20 parts by weight of chromium-enriched yeast respectively, and then put them into a mixer and mix them for 40 to 50 minutes;

[0038] (2) The mixed materials are crushed using a crusher for 5 to 10 minutes, and the crushed materials are sieved through a 60 to 70 mesh screen;

[0039] (3) The sieved material is mixed for a second time in a mixer for 40 to 50 minutes, the material obtained after the second mixing is collected, and dry-pressed and granulated by a granulator at a pressure of 8 to 12 kN / cm. The granules obtained by dry-pressing and granulating are collected and packaged to obtain the product.

[0040] The preparation process of the cherry blossom extract is as follows: after the cherry blossoms are picked, they are soaked and extracted at a temperature of 55 to 65°C for 2 to 3 hours in 45% to 55% ethanol by mass, the extract is initially filtered using a ceramic membrane and then separated and concentrated using a nanofiltration membrane, the concentrated solution is collected and diluted with water (the amount of water added here is not limited, it is only used for dilution, and the subsequent adsorption, water washing and analysis operations can be carried out smoothly), the diluted feed liquid is adsorbed and washed with water using a macroporous resin (the amount of water for washing here is not limited, and water can be added repeatedly), and analyzed with 75% to 85% ethanol by mass, the analyzed solution is collected, replenished with water and concentrated (the amount of water added here is not limited, it is mainly to increase the amount of liquid, facilitate concentration, and enable smooth spray drying), spray dried, collected the obtained spray dry powder, crushed and sieved to obtain a dry powder cherry blossom extract, wherein the total flavonoid content of cherry blossoms is ≥30%. The parameters of the ceramic membrane are a ceramic membrane with a pore size of 8 to 10 μm, the parameters of the nanofiltration membrane are a nanofiltration membrane with a pore size of 1 to 4 nm, the parameters of the macroporous resin are D101 macroporous resin, the parameters of the spray drying are an inlet air temperature of 180 to 220° C., an outlet air temperature of 100 to 140° C., and the mesh size of the sieving screen is 60 to 70 meshes.

[0041] The preparation process of the cinnamon extract is that the cinnamon medicinal material is roughly crushed, and then refluxed and extracted with 75% to 85% ethanol by mass for 1 to 5 times, the extract is collected and filtered, and the filtrate is collected and concentrated after adding water (the amount of water added here is not limited, mainly to increase the amount of liquid, facilitate concentration, and enable smooth spray drying), to obtain a concentrated solution, after instantaneous high-temperature sterilization, the concentrated solution is spray-dried, the obtained spray-dried powder is collected, and after crushing and sieving, a dry powder cinnamon extract is obtained, wherein the cinnamon polyphenol content is ≥30%. The temperature of the instantaneous high-temperature sterilization is 120 to 150° C., the parameters of the spray drying are an inlet air temperature of 180 to 220° C., an outlet air temperature of 100 to 140° C., and the mesh number of the sieve is 60 to 70 meshes.

[0042] The preparation process of the bitter melon extract is that the bitter melon is chopped, water is added and refluxed for 1 to 5 times (the amount of water added is not limited here, as long as reflux can be achieved), the extract is filtered, the filtrate is cooled to room temperature and then enzymolyzed with protease, the feed liquid is boiled and inactivated after enzymolysis, the enzymolyzed liquid is concentrated, and then sterilized at instantaneous high temperature, the sterilized liquid is spray-dried, the spray-dried powder is collected, and the dry powder bitter melon extract is obtained after crushing and sieving, wherein the bitter melon polypeptide content is ≥60%. The specific process and parameters involved in the protease enzymolysis are adding 0.5% protease by mass to the extract cooled to 30 to 40°C, maintaining the temperature at 45 to 55°C and stirring the enzymolysis for 20 to 40 minutes, the temperature of the instantaneous high temperature sterilization is 120 to 150°C, the parameters of the spray drying are the inlet air temperature of 180 to 220°C, the outlet air temperature of 100 to 140°C, and the mesh number of the sieved screen is 60 to 70 meshes.

[0043] The mulberry leaf extract is a dry powder of mulberry leaf extract obtained after mulberry leaves are crushed, extracted with water, ultrafiltered, nanofiltered, concentrated and dried. The content of its index component 1-deoxynojirimycin is ≥1%. The mulberry leaf extract is purchased from Shanghai Haoyun Biotechnology Co., Ltd., and the product batch number is: 20240101.

[0044] The chromium-rich yeast is a yeast dry powder that is enriched and cultured using raw materials rich in chromium and dried, and its organic chromium content is ≥2000 mg / kg. The chromium-rich yeast is purchased from Zhejiang Shenyou Biotechnology Co., Ltd., and the product batch number is: SY33.802-20220917.

[0045] Example 1 Preparation of cherry blossom extract

[0046] The preparation process of the cherry blossom extract described in this embodiment is to soak and extract the cherry blossoms with 50% ethanol by mass at 60°C for 2.5 hours after the cherry blossoms are picked, and then the extract is filtered using a ceramic membrane and then separated and concentrated using a nanofiltration membrane, and the concentrated solution is collected and diluted with water, and the diluted feed liquid is adsorbed by a macroporous resin, washed with water, and analyzed with 80% ethanol by mass, and the analyzed solution is collected and concentrated by water, and then spray-dried and collected to obtain a dry powder cherry blossom extract after crushing and sieving, wherein the total flavonoid content of cherry blossoms is ≥30%. The parameters of the ceramic membrane are a ceramic membrane with a pore size of 9μm, the parameters of the nanofiltration membrane are a nanofiltration membrane with a pore size of 2nm, the parameters of the macroporous resin are D101 macroporous resin, the parameters of the spray drying are an inlet air temperature of 200°C, an outlet air temperature of 120°C, and the mesh number of the sieved screen is 60 meshes.

[0047] Example 2 Preparation of cherry blossom extract

[0048] The preparation process of the cherry blossom extract described in this embodiment is to soak and extract the cherry blossoms with 55% ethanol by mass at 55°C for 3 hours after picking, and then use a ceramic membrane to filter the extract and then use a nanofiltration membrane to separate and concentrate it, collect the concentrated solution and dilute it with water, use a macroporous resin to adsorb the diluted solution, wash it with water, and analyze it with 85% ethanol by mass, collect the analyzed solution, replenish water and concentrate it, spray dry it, collect the spray dry powder, and crush and sieve it to obtain a dry powder cherry blossom extract, wherein the total flavonoid content of cherry blossoms is ≥30%. The parameters of the ceramic membrane are 8μm ceramic membrane with pore size, the parameters of the nanofiltration membrane are 1nm nanofiltration membrane with pore size, the parameters of the macroporous resin are D101 macroporous resin, the parameters of the spray drying are 180°C inlet air temperature, 100°C outlet air temperature, and the mesh number of the sieved screen is 60 meshes.

[0049] Example 3 Preparation of cherry blossom extract

[0050] The preparation process of the cherry blossom extract described in this embodiment is to soak and extract the cherry blossoms with 45% ethanol by mass at 65°C for 2 hours after the cherry blossoms are picked, and then the extract is filtered using a ceramic membrane and then separated and concentrated using a nanofiltration membrane, and the concentrated solution is collected and diluted with water, and the diluted feed liquid is adsorbed by a macroporous resin, washed with water, and analyzed with 75% ethanol by mass, and the analyzed solution is collected and concentrated by water, and then spray-dried and collected to obtain a dry powder cherry blossom extract after crushing and sieving, wherein the total flavonoid content of cherry blossoms is ≥30%. The parameters of the ceramic membrane are a ceramic membrane with a pore size of 10μm, the parameters of the nanofiltration membrane are a nanofiltration membrane with a pore size of 4nm, the parameters of the macroporous resin are D101 macroporous resin, the parameters of the spray drying are an inlet air temperature of 220°C, an outlet air temperature of 140°C, and the mesh number of the sieved screen is 70 meshes.

[0051] Example 4 Preparation of cinnamon extract

[0052] The preparation process of the cinnamon extract described in this embodiment is to grind the cinnamon medicinal material into pieces, extract it twice with 80% ethanol by mass, collect the extract and filter it, collect the filtrate and add water to concentrate it to obtain a concentrated solution, sterilize it at instantaneous high temperature, spray dry the concentrated solution, collect the spray dry powder, grind and sieve it to obtain a dry powdered cinnamon extract, wherein the cinnamon polyphenol content is ≥30%. The temperature of the instantaneous high temperature sterilization is 130°C, the parameters of the spray drying are an inlet air temperature of 200°C, an outlet air temperature of 130°C, and the mesh number of the sieve is 60 meshes.

[0053] Example 5 Preparation of cinnamon extract

[0054] The preparation process of the cinnamon extract described in this embodiment is to grind the cinnamon medicinal material into pieces, extract it once with 85% ethanol by mass, collect the extract and filter it, collect the filtrate and add water to concentrate it to obtain a concentrated solution, sterilize it at instantaneous high temperature, spray dry the concentrated solution, collect the spray dry powder, grind and sieve it to obtain a dry powdered cinnamon extract, wherein the cinnamon polyphenol content is ≥30%. The temperature of the instantaneous high temperature sterilization is 120°C, the parameters of the spray drying are an inlet air temperature of 180°C, an outlet air temperature of 100°C, and the mesh number of the sieve is 60 meshes.

[0055] Example 6 Preparation of cinnamon extract

[0056] The preparation process of the cinnamon extract described in this embodiment is to grind the cinnamon medicinal material into pieces, extract it with 75% ethanol by mass for 5 times, collect the extract and filter it, collect the filtrate and add water to concentrate it to obtain a concentrated solution, sterilize it at instantaneous high temperature, spray dry the concentrated solution, collect the spray dry powder, grind and sieve it to obtain a dry powdered cinnamon extract, wherein the cinnamon polyphenol content is ≥30%. The temperature of the instantaneous high temperature sterilization is 150°C, the parameters of the spray drying are an inlet air temperature of 220°C, an outlet air temperature of 140°C, and the mesh number of the sieve is 70 meshes.

[0057] Example 7 Preparation of Momordica charantia Extract

[0058] The preparation process of the bitter melon extract described in this embodiment is that the bitter melon is chopped, water is added to reflux and extracted three times, the extract is filtered, the filtrate is cooled to room temperature and then enzymatically hydrolyzed with protease, the feed liquid is boiled and inactivated after enzymatic hydrolysis, the enzymatic hydrolyzate is concentrated, and then sterilized at instantaneous high temperature, and then the sterilized liquid is spray-dried, the spray-dried powder is collected, and the dry powder bitter melon extract is obtained after crushing and sieving, wherein the bitter melon polypeptide content is ≥60%. The specific process and parameters involved in the protease hydrolysis are adding 0.5% protease by mass to the extract cooled to 35°C, stirring the enzymatic hydrolysis for 30 minutes at a temperature of 50°C, the temperature of the instantaneous high temperature sterilization is 130°C, the parameters of the spray drying are the inlet air temperature of 200°C, the outlet air temperature of 130°C, and the mesh number of the sieved screen is 60 meshes.

[0059] Example 8 Preparation of Momordica charantia Extract

[0060] The preparation process of the bitter melon extract described in this embodiment is that the bitter melon is chopped, water is added to reflux and extracted once, the extract is filtered, the filtrate is cooled to room temperature and then enzymatically hydrolyzed with protease, the enzymatically hydrolyzed liquid is boiled and inactivated, the enzymatically hydrolyzed liquid is concentrated, and then sterilized at a high temperature for an instant, and then the sterilized liquid is spray-dried, the spray-dried powder is collected, and the dry powder bitter melon extract is obtained after crushing and sieving, wherein the bitter melon polypeptide content is ≥60%. The specific process and parameters involved in the protease hydrolysis are to add 0.5% protease by mass to the extract cooled to 30°C, stir the enzymatic hydrolysis at 45°C for 40 minutes, the temperature of the instantaneous high temperature sterilization is 120°C, the parameters of the spray drying are the inlet air temperature of 180°C, the outlet air temperature of 100°C, and the mesh number of the sieved screen is 70 meshes.

[0061] Example 9 Preparation of Momordica charantia Extract

[0062] The preparation process of the bitter melon extract described in this embodiment is that the bitter melon is chopped, water is added to reflux and extracted 5 times, the extract is filtered, the filtrate is cooled to room temperature and then enzymatically hydrolyzed with protease, the feed liquid is boiled and inactivated after enzymatic hydrolysis, the enzymatic hydrolyzate is concentrated, and then sterilized at instantaneous high temperature, and then the sterilized liquid is spray-dried, the spray-dried powder is collected, and the dry powder bitter melon extract is obtained after crushing and sieving, wherein the bitter melon polypeptide content is ≥60%. The specific process and parameters involved in the egg protease enzymatic hydrolysis are adding 0.5% protease by mass to the extract cooled to 40°C, maintaining the temperature at 55°C and stirring the enzymatic hydrolysis for 20 minutes, the temperature of the instantaneous high temperature sterilization is 150°C, the parameters of the spray drying are the inlet air temperature of 220°C, the outlet air temperature of 140°C, and the mesh number of the sieved screen is 60 meshes.

[0063] Example 10

[0064] A composition with hypoglycemic function comprises the following components in parts by weight: 10 parts by weight of cherry blossom extract, 150 parts by weight of cinnamon extract, 5 parts by weight of bitter melon extract, 75 parts by weight of mulberry leaf extract, and 10 parts by weight of chromium-enriched yeast. The cherry blossom extract is prepared in Example 1, the cinnamon extract is prepared in Example 4, and the bitter melon extract is prepared in Example 7.

[0065] The preparation method of the above-mentioned composition having the function of lowering blood sugar comprises the following steps:

[0066] (1) Weigh 10 parts by weight of cherry blossom extract, 150 parts by weight of cinnamon extract, 5 parts by weight of bitter melon extract, 75 parts by weight of mulberry leaf extract, and 10 parts by weight of chromium-enriched yeast respectively, and then put them into a mixer and mix them for 45 minutes;

[0067] (2) The mixed materials were crushed using a crusher for 7 minutes, and the crushed materials were sieved through a 60-mesh screen;

[0068] (3) The sieved material is mixed for a second time using a mixer for 45 minutes, the material obtained after the second mixing is collected, and dry-pressed and granulated by a granulator at a pressure of 10 kN / cm. The granules obtained by dry-pressing and granulating are collected and packaged to obtain the product.

[0069] Embodiment 11

[0070] A composition with hypoglycemic function comprises the following components in parts by weight: 20 parts by weight of cherry blossom extract, 175 parts by weight of cinnamon extract, 5 parts by weight of bitter melon extract, 90 parts by weight of mulberry leaf extract, and 20 parts by weight of chromium-enriched yeast. The cherry blossom extract is prepared in Example 2, the cinnamon extract is prepared in Example 5, and the bitter melon extract is prepared in Example 8.

[0071] The preparation method of the above-mentioned composition having the function of lowering blood sugar comprises the following steps:

[0072] (1) Weigh 20 parts by weight of cherry blossom extract, 175 parts by weight of cinnamon extract, 5 parts by weight of bitter melon extract, 90 parts by weight of mulberry leaf extract, and 20 parts by weight of chromium-enriched yeast respectively, and put them into a mixer and mix them for 50 minutes;

[0073] (2) The mixed materials were crushed using a crusher for 10 minutes, and the crushed materials were sieved through a 70-mesh screen;

[0074] (3) The sieved material is mixed for a second time using a mixer for 50 minutes, the material obtained after the second mixing is collected, and dry-pressed and granulated by a granulator at a pressure of 12 kN / cm. The granules obtained by dry-pressing and granulating are collected and packaged to obtain the product.

[0075] Example 12

[0076] A composition with hypoglycemic function comprises the following components in parts by weight: 5 parts by weight of cherry blossom extract, 128 parts by weight of cinnamon extract, 1 part by weight of bitter melon extract, 60 parts by weight of mulberry leaf extract, and 5 parts by weight of chromium-enriched yeast. The cherry blossom extract is prepared in Example 1, the cinnamon extract is prepared in Example 4, and the bitter melon extract is prepared in Example 7.

[0077] The preparation method of the above-mentioned composition having the function of lowering blood sugar comprises the following steps:

[0078] (1) Weigh 5 parts by weight of cherry blossom extract, 128 parts by weight of cinnamon extract, 1 part by weight of bitter melon extract, 60 parts by weight of mulberry leaf extract, and 5 parts by weight of chromium-enriched yeast respectively, and then put them into a mixer and mix them for 50 minutes;

[0079] (2) The mixed materials were crushed using a crusher for 5 minutes, and the crushed materials were sieved through a 60-mesh screen;

[0080] (3) The sieved material is mixed for a second time using a mixer for 40 minutes, the material obtained after the second mixing is collected, and dry-pressed and granulated by a granulator at a pressure of 8 kN / cm. The granules obtained by dry-pressing and granulating are collected and packaged to obtain the product.

[0081] Performance Testing:

[0082] (i) Prepare samples 1-10 according to the parameters in Table 1.

[0083] The source of the cherry blossom extract was prepared in Example 1, the source of the cinnamon extract was prepared in Example 4, the source of the bitter melon extract was prepared in Example 7, and the mulberry leaf extract and chromium-enriched yeast were purchased from outside. Sample 10 is a sample of the composition obtained in Example 10, and the preparation method of samples 5-9 is the same as that of Example 10.

[0084] Table 1 Composition parameters of samples 1-10

[0085]

[0086]

[0087] (II) α-glucosidase inhibition test of samples 1-10

[0088] 2.1 Reagent configuration:

[0089] α-Glucosidase solution: 1 mol / mL;

[0090] pNPG solution (4-nitrophenyl-α-D-furanoglucoside): 5 mmol / mL (PBS solution at pH 6.87);

[0091] Sodium carbonate solution: 0.1 mol / mL;

[0092] PBS solution: 0.2 mol / mL (pH 6.8);

[0093] Acarbose solution: 0.2mg / ml.

[0094] 2.2 Detection method:

[0095] Experimental group: Take 250 μL of the test sample aqueous solution, add 250 μL of pNPG solution and 250 μL of PBS solution, mix well and place at 37°C for 10 min; add 250 μL of α-glucosidase solution, mix well and place at 37°C for 10 min; add 500 μL of sodium carbonate solution, mix well and measure its absorbance at a wavelength of 405 nm or 450 nm.

[0096] Control group: Take 250 μL of aqueous solution, add 250 μL of pNPG solution and 250 μL of PBS solution, mix well and place at 37°C for 10 min; add 250 μL of α-glucosidase solution, mix well and place at 37°C for 10 min; add 500 μL of sodium carbonate solution, mix well and measure its absorbance at 405 nm or 450 nm.

[0097] Background group: add 250 μL pNPG solution and 250 μL PBS solution to 250 μL of the test sample aqueous solution, mix well and place at 37°C for 20 min; add 500 μL sodium carbonate solution, mix well and measure the absorbance at 405 nm or 450 nm.

[0098] 2.3 Calculation formula:

[0099] Clearance = 1-(A1-A2) / A0*100%

[0100] Where: A0 represents the absorbance of the control group; A1 represents the absorbance of the experimental group; A2 represents the absorbance of the background group.

[0101] 2.4 α-Glucosidase inhibition rate experimental results

[0102] from Figure 1 It can be seen from the experimental results in Table 2 that the values ​​of the α-glucosidase inhibition rate of samples 7-10 are all higher than the positive control acarbose. Among them, sample 10 (the finished product of the composition) has the highest α-glucosidase inhibition rate, which is better than any other group of samples, especially in the case of high concentration, and its α-glucosidase inhibition rate shows a significant advantage, which is much higher than other raw material combinations, showing the superiority of the composition of the present invention in blood sugar control.

[0103] The α-glucosidase inhibition rates of samples 1-6 were lower than those of acarbose. Samples 1-5 were all single-ingredient or dual-ingredient products, and sample 6 contained three raw materials: cherry blossom, mulberry leaf, and chromium-rich yeast. The α-glucosidase inhibition rates of these samples were lower than those of the positive control acarbose, possibly due to the limitations of the single-ingredient or dual-ingredient ingredients on the blood sugar control function or mechanism.

[0104] Table 2 α-glucosidase inhibition rate (%) of samples 1-10 Experimental results

[0105]

[0106] (III) α-amylase inhibition test of samples 1-10

[0107] 3.1 Reagent configuration:

[0108] α-Amylase solution: 7 mol / mL (prepared with PBS solution);

[0109] PBS solution: 0.2 mol / mL (pH 6.8);

[0110] DNS reagent: Dissolve 91g of potassium sodium tartrate in 250ml of distilled water, heat (not exceeding 50°C) to dissolve, add 3.15g of 3,5-dinitrosalicylic acid, 10.5g of sodium hydroxide, 2.5g of phenol, and 2.5g of anhydrous sodium sulfite in sequence, stir and dissolve, cool and then dilute to 1000mL;

[0111] 5% soluble starch solution: Dissolve 5g soluble starch in 100ml distilled water, boil until clear, and cool to room temperature for later use;

[0112] Acarbose solution: 0.2mg / ml.

[0113] 3.2 Detection method:

[0114] Experimental group: Take 50 μL of the sample solution and 50 μL of α-amylase solution and add them into a 5 mL centrifuge tube. After reacting at 37 °C for 10 min, add 500 μL of 5% starch solution and react at 37 °C for 10 min. Finally, add 500 μL of DNS reagent and terminate the reaction in a boiling water bath for 5 min. After cooling, dilute to 25 mL and measure the absorbance at 540 nm.

[0115] Control group: Take 50μL PBS solution and 50μL α-amylase solution and add them into a 5mL centrifuge tube. After reacting at 37℃ for 10min, add 500μL 5% starch solution and react at 37℃ for 10min. Finally, add 500μL DNS reagent and terminate the reaction in a boiling water bath for 5min. After cooling, dilute to 25mL and measure the absorbance at 540nm.

[0116] Background group: Take 50 μL of the sample solution to be tested and add it to a 5 mL centrifuge tube. After reacting at 37 °C for 10 min, add 500 μL of 5% starch solution and react at 37 °C for 10 min. Finally, add 500 μL of DNS reagent and boil in water for 5 min to terminate the reaction. After cooling, dilute to 25 mL and measure the absorbance at 540 nm.

[0117] 3.3 Calculation formula:

[0118] Clearance = 1-(A1-A2) / A0*100%

[0119] Where: A0 represents the absorbance of the control group; A1 represents the absorbance of the experimental group; A2 represents the absorbance of the background group.

[0120] 3.4 Experimental Results

[0121] from Figure 2 It can be seen from the experimental data in Table 3 that the results of the α-amylase inhibition rate experiment are basically consistent with the α-glucosidase inhibition rate experiment. Although some samples show different inhibition rate effects at different concentrations, the basic trends are consistent. The values ​​of samples 7, 8, 9, and 10 are all higher than the positive control acarbose. Among them, sample 10 (the finished product of the composition) has the highest α-amylase inhibition rate value, which is better than any other group of samples, especially in the case of high concentrations. It shows a significant advantage in the α-amylase inhibition rate, which is much higher than other raw material combinations, showing the superiority of the finished product formula of the composition in blood sugar control.

[0122] The α-amylase inhibition rates of samples 1-6 were all lower than those of acarbose. Samples 1-5 were all single-ingredient or dual-ingredient products, and sample 6 contained three raw materials: cherry blossoms, mulberry leaves, and chromium-rich yeast. The α-amylase inhibition rates of these samples were lower than those of the positive control acarbose, possibly due to the limitations of the single-ingredient or dual-ingredient ingredients on the blood sugar control function or mechanism.

[0123] Table 3 α-amylase inhibition rate (%) of samples 1-10 Experimental results

[0124]

[0125] The blood sugar lowering composition of the present invention is only presented in the form of granules, and the dosage form can also be changed according to actual needs to be prepared into tablets, capsules and other finished preparations. The quality standards of raw materials and the proportion of the formula are basically consistent with the current ones.

[0126] The above contents are further detailed descriptions of the present invention in combination with specific preferred embodiments, and the specific implementation of the present invention cannot be limited to these descriptions. For ordinary technicians in the field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the protection scope of the present invention.

Claims

1. A composition having a blood sugar lowering function, characterized in that: The composition comprises the following components in parts by weight: 5 to 20 parts by weight of cherry blossom extract, 128-175 parts by weight of cinnamon extract, 1 to 5 parts by weight of bitter melon extract, 60-90 parts by weight of mulberry leaf extract, 5 to 20 parts by weight of chromium-enriched yeast.

2. The composition having hypoglycemic function according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 10 parts by weight of cherry blossom extract, 150 parts by weight of cinnamon bark extract, 5 parts by weight of bitter melon extract, 75 parts by weight of mulberry leaf extract and 10 parts by weight of chromium-enriched yeast.

3. The method for preparing the composition with hypoglycemic function according to claim 1, characterized in that: The steps include: (1) Weigh 5 to 20 parts by weight of cherry blossom extract, 128 to 175 parts by weight of cinnamon extract, 1 to 5 parts by weight of bitter melon extract, 60 to 90 parts by weight of mulberry leaf extract, and 5 to 20 parts by weight of chromium-enriched yeast respectively, and then put them into a mixer for mixing; (2) The mixed materials are crushed by a crusher, and the crushed materials are sieved through a sieve; (3) The sieved materials are mixed for a second time using a mixer, the materials obtained after the second mixing are collected, and the materials are dry-pressed and granulated using a granulator, and the granules obtained by dry-pressing and granulating are collected and packaged to obtain the product.

4. The method for preparing the composition having hypoglycemic function according to claim 3, characterized in that: The mixing time in step (1) is 40 to 50 minutes; the crushing time in step (2) is 5 to 10 minutes; the mesh size of the sieve is 60 to 70 meshes; the secondary mixing time in step (3) is 40 to 50 minutes, and the dry pressing pressure is 8 to 12 kN / cm.

5. The method for preparing the composition having hypoglycemic function according to claim 3, characterized in that: The preparation process of the cherry blossom extract is as follows: after cherry blossoms are picked, they are soaked and extracted in 45% to 55% ethanol at a temperature of 55 to 65° C. for 2 to 3 hours, the extract is initially filtered using a ceramic membrane and then separated and concentrated using a nanofiltration membrane, the concentrated solution is collected and diluted with water, the diluted solution is adsorbed using a macroporous resin, washed with water, and analyzed with 75% to 85% ethanol by mass, the analyzed solution is collected, replenished with water, concentrated, spray-dried, collected, and crushed and sieved to obtain the cherry blossom extract.

6. The method for preparing the composition having hypoglycemic function according to claim 5, characterized in that: The parameters of the ceramic membrane are a ceramic membrane with a pore size of 8 to 10 μm, the parameters of the nanofiltration membrane are a nanofiltration membrane with a pore size of 1 to 4 nm, the parameters of the macroporous resin are D101 macroporous resin, the parameters of the spray drying are an inlet air temperature of 180 to 220° C., an outlet air temperature of 100 to 140° C., and the mesh size of the sieving screen is 60 to 70 meshes.

7. The method for preparing the composition having hypoglycemic function according to claim 3, characterized in that: The preparation process of the cinnamon extract is as follows: the cinnamon medicinal material is roughly crushed, and then refluxed and extracted 1 to 5 times with 75% to 85% ethanol by mass, the extract is collected and filtered, the filtrate is collected and concentrated after adding water to obtain a concentrated solution, after instant high-temperature sterilization, the concentrated solution is spray-dried, the obtained spray-dried powder is collected, and the cinnamon extract is obtained after crushing and sieving.

8. The method for preparing the composition having hypoglycemic function according to claim 7, characterized in that: The temperature of the instantaneous high temperature sterilization is 120-150° C., the parameters of the spray drying are an inlet air temperature of 180-220° C., an outlet air temperature of 100-140° C., and the mesh number of the sieving screen is 60-70 meshes.

9. The method for preparing the composition having hypoglycemic function according to claim 3, characterized in that: The preparation process of the bitter melon extract is as follows: the bitter melon is chopped, water is added for reflux extraction for 1 to 5 times, the extract solution is filtered, the filtrate is cooled to room temperature and then enzymatically hydrolyzed with a protease, the enzymatically hydrolyzed liquid is boiled and inactivated, the enzymatically hydrolyzed liquid is concentrated, sterilized at high temperature for an instant, the sterilized liquid is spray-dried, the obtained spray-dried powder is collected, and the bitter melon extract is obtained after being crushed and sieved.

10. The method for preparing the composition having hypoglycemic function according to claim 9, characterized in that: The specific process and parameters involved in the protease hydrolysis are adding 0.5% by mass of protease to the extract cooled to 30-40°C, maintaining the temperature at 45-55°C and stirring the hydrolysis for 20-40 minutes, the temperature of the instantaneous high temperature sterilization is 120-150°C, the parameters of the spray drying are an inlet air temperature of 180-220°C, an outlet air temperature of 100-140°C, and the mesh number of the sieving screen is 60-70 meshes.

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