A fluorescence imaging method and device based on light source intensity adjustment and a storage medium

Abstract

The application provides a fluorescence imaging method and device based on light source intensity adjustment and a storage medium, the method comprising: in a PDD fluorescence mode, when a blue light source is turned on, after the blue light is reflected by an observed tissue, the spectrum of green fluorescence bands generated by the excitation of riboflavin is filtered through a trap wave filter; in a photodynamic white light image mode, the red, green and blue light emitted by the red, green and blue light sources all passes through a light compensation module for pre-set light power adjustment, so that the light reflected by the observed tissue and then passing through the trap wave filter is white light, the light compensation module controls the light power of the red light and the blue light output to be consistent, and the green light output power is higher than the light power of the red light or the blue light. Through the combination of the trap wave filter and the light compensation module, the problem that the green fluorescence generated by the excitation of riboflavin affects the contrast of PDD fluorescence imaging is solved, and the color restoration of traditional white light imaging can be ensured.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of photodynamic diagnosis, and in particular to a fluorescence imaging method and device based on light source intensity adjustment and a storage medium. BACKGROUND

[0002] Photo dynamic diagnosis (PDD) is a new type of diagnostic technique, which allows photosensitizing substances to accumulate in diseased tissues and emit fluorescence when excited by light, thereby determining the presence or absence of disease and diseased tissues.

[0003] This technique is widely used in the field of medical diagnosis. For example, in the diagnosis of tumors, photosensitive drugs are selectively enriched at cancer cells; then the cancer tissue is irradiated with excitation light, causing the enriched photosensitive drugs to produce fluorescence; by analyzing the intensity and distribution of the fluorescence, the location and condition of the cancer can be determined. For another example, in the process of bladder PDD imaging, blue-violet light is used as excitation light to irradiate photosensitizing agents in the tissue, exciting red fluorescence and imaging it.

[0004] However, in the process of bladder PDD imaging, the metabolite of urine, riboflavin, will be excited by excitation light to emit green fluorescence, thereby interfering with the contrast of PDD fluorescence. The existing method is to change the excitation light wavelength, such as using 500nm as the photosensitizer excitation wavelength, to reduce the excitation efficiency of riboflavin. However, changing the excitation wavelength also causes the fluorescence excitation efficiency of photosensitizers to decrease, and the fluorescence brightness of PDD imaging to decrease. At the same time, it causes more loss of visible light imaging band, which is not conducive to traditional white light imaging. SUMMARY

[0005] The present application provides a fluorescence imaging method based on light source intensity adjustment, which combines a trap wave filter and a light compensation module to solve the problem of green fluorescence caused by riboflavin being excited affecting the contrast of PDD fluorescence imaging during the PDD fluorescence imaging process, while also ensuring the color restoration of traditional white light imaging.

[0006] Therefore, the application provides a fluorescence imaging method based on light source intensity adjustment, which is applied to a light intensity adjusted fluorescence imaging device, the light intensity adjusted fluorescence imaging device includes two modes of a photodynamic diagnosis (PDD) fluorescence mode and a photodynamic white light image mode, and the method includes the following steps.

[0007] With reference to the first aspect, in a possible implementation manner, the red, green and blue light emitted by the red, green and blue light sources is adjusted by the light compensation module according to the pre-set light power ratio, and the ratio of the red, green and blue light in the pre-set light power ratio is related to the transmittance of the trap wave filter.

[0008] With reference to the first aspect, in a possible implementation manner, the ratio of the red, green and blue light in the pre-set light power ratio is related to the transmittance of the trap wave filter, and specifically, if it is determined that the transmittance ratio of the trap wave filter to the red, green and blue light is a:b:a, the output power of the red light spectrum, the green light spectrum and the blue light spectrum is controlled by the light compensation module to be b:a:b, and a and b are positive numbers.

[0009] With reference to the first aspect, in a possible implementation manner, the red, green and blue light emitted by the red, green and blue light sources enters the light sensing element with the light power of P

[0010]

[0011] wherein, P r is the light power of the red light entering the light sensing element, P g is the light power of the green light entering the light sensing element, P b is the light power of the blue light entering the light sensing element, λ is the wavelength of light, L r (λ) is the output power of the red light spectrum, L g (λ) is the output power of the green light spectrum, and L b (λ) is the output power of the blue light spectrum.

[0012] With reference to the first aspect, in a possible implementation manner, when in the light dynamic white light image mode, the light compensation module controls output powers of red, green and blue light so that the difference percentage of the red, green and blue light powers entering the photosensitive element and the average power of the three color lights is less than or equal to a preset difference percentage threshold.

[0013] In a possible implementation manner, the average power of the red, green and blue light is

[0014]

[0015] The difference percentage of the red light power and the average power of the three color lights is

[0016]

[0017] The difference percentage of the green light power and the average power of the three color lights is

[0018]

[0019] The difference percentage of the blue light power and the average power of the three color lights is

[0020]

[0021] wherein, P r is the red light power, P g is the green light power, P b is the blue light power, ΔP r is the difference of the red light power and the average power of the three color lights, ΔP g is the difference of the green light power and the average power of the three color lights, and ΔP b is the difference of the blue light power and the average power of the three color lights, a is the preset difference percentage threshold, and 0 < a < 1.

[0022] ​The second aspect of the application provides a fluorescence imaging device based on light source intensity adjustment, characterized in that the device comprises a blue light source 1, a green light source 2, a red light source 3, a light compensation module 4, an imaging lens group 5, a trap wave filter 6, a photosensitive element 7, and an image processor 8; in a photodynamic diagnosis PDD fluorescence mode, the blue light source 1 is turned on, and the green light source 2 and the red light source 3 are turned off; after the blue light emitted by the blue light source 1 irradiates the observed tissue, the imaging lens group 5 is used to collect the spectrum of the PDD fluorescence generated by the excited observed tissue and the green fluorescence generated by the riboflavin; the trap wave filter 6 is used to filter the riboflavin fluorescence, and the PDD fluorescence transmits through the trap wave filter 6 to the photosensitive element 7; the photosensitive element 7 is used to convert the optical signal into an electrical signal and transmit it to the image processor 8 for processing, so as to output a PDD fluorescence image; in a photodynamic white light image mode, the blue light source 1, the green light source 2, and the red light source 3 are all turned on under the control of the light compensation module 4, and the red, green, and blue light emitted by the blue light source 1, the green light source 2, and the red light source 3 all pass through the light compensation module 4 for pre-set light power adjustment; after the three-color light is reflected by the observed tissue, the reflected light is collected by the imaging lens group 5, and then enters the photosensitive element 7 after being filtered by the trap wave filter 6; the photosensitive element 7 is used to convert the optical signal into an electrical signal and transmit it to the image processor 8 for processing, so as to output a white light image; the light compensation module 4 controls the light power output by the blue light source 1 and the red light source 3 to be consistent, and the output power of the green light source 2 is higher than that of the blue light source 1 or the red light source 3.

[0023] In combination with the second aspect, in a possible implementation manner, the light compensation module 4 adjusts the light output of the red, green, and blue three-color light according to a pre-set light power ratio, wherein the ratio of the red, green, and blue three colors of the pre-set light power ratio is related to the transmittance of the trap wave filter 6.

[0024] In combination with the second aspect, in a possible implementation manner, if it is determined that the transmittance ratio of the red, green, and blue three-color light of the trap wave filter 6 is a:b:a, then the output power of the red light spectrum, the green light spectrum, and the blue light spectrum is controlled by the light compensation module 4 to be b:a:b, and a and b are positive numbers.

[0025] In combination with the second aspect, in a possible implementation manner, 10. The fluorescence imaging device based on light source intensity adjustment according to claim 9 is characterized in that,

[0026] The light power of the red, green, and blue light emitted by the red, green, and blue light sources entering the photosensitive element 7 is respectively:

[0027]

[0028] wherein Pr P red is the light power of red light entering the photosensitive element 7, λ is the wavelength of light, L g P green is the light power of green light entering the photosensitive element 7, λ is the wavelength of light, L b P blue is the light power of blue light entering the photosensitive element 7, λ is the wavelength of light, L r P red (λ) is the output power of the red light spectrum, L g P green (λ) is the output power of the green light spectrum, L b P blue (λ) is the output power of the blue light spectrum.

[0029] With reference to the second aspect, in a possible implementation manner, when in the light-driven white light image mode, the light compensation module 4 controls the output powers of red, green and blue light so that the difference percentage of the red, green and blue light powers entering the photosensitive element 7 and the average power of the three colors of light is less than or equal to a preset difference percentage threshold.

[0030] With reference to the second aspect, in a possible implementation manner, the average power of the red, green and blue light is

[0031]

[0032] The difference percentage of the red light power and the average power of the three colors of light is

[0033]

[0034] The difference percentage of the green light power and the average power of the three colors of light is

[0035]

[0036] The difference percentage of the blue light power and the average power of the three colors of light is

[0037]

[0038] P red is the red light power, P green is the green light power, P blue is the blue light power, ΔP red is the difference between the red light power and the average power of the three colors of light, ΔP green is the difference between the green light power and the average power of the three colors of light, ΔP blue is the difference between the blue light power and the average power of the three colors of light, and a is the preset difference percentage threshold, 0 < a < 1. r P red is the red light power, P green is the green light power, P blue is the blue light power, ΔP red is the difference between the red light power and the average power of the three colors of light, ΔP green is the difference between the green light power and the average power of the three colors of light, ΔP blue is the difference between the blue light power and the average power of the three colors of light, and a is the preset difference percentage threshold, 0 < a < 1. g P red is the red light power, P green is the green light power, P blue is the blue light power, ΔP red is the difference between the red light power and the average power of the three colors of light, ΔP green is the difference between the green light power and the average power of the three colors of light, ΔP blue is the difference between the blue light power and the average power of the three colors of light, and a is the preset difference percentage threshold, 0 < a < 1. b P red is the red light power, P green is the green light power, P blue is the blue light power, ΔP red is the difference between the red light power and the average power of the three colors of light, ΔP green is the difference between the green light power and the average power of the three colors of light, ΔP blue is the difference between the blue light power and the average power of the three colors of light, and a is the preset difference percentage threshold, 0 < a < 1. r P red is the red light power, P green is the green light power, P blue is the blue light power, ΔP red is the difference between the red light power and the average power of the three colors of light, ΔP green is the difference between the green light power and the average power of the three colors of light, ΔP blue is the difference between the blue light power and the average power of the three colors of light, and a is the preset difference percentage threshold, 0 < a < 1. g P red is the red light power, P green is the green light power, P blue is the blue light power, ΔP red is the difference between the red light power and the average power of the three colors of light, ΔP green is the difference between the green light power and the average power of the three colors of light, ΔP blue is the difference between the blue light power and the average power of the three colors of light, and a is the preset difference percentage threshold, 0 < a < 1. b P red is the red light power, P green is the green light power, P blue is the blue light power, ΔP red is the difference between the red light power and the average power of the three colors of light, ΔP green is the difference between the green light power and the average power of the three colors of light, ΔP blue is the difference between the blue light power and the average power of the three colors of light, and a is the preset difference percentage threshold, 0 < a < 1.

[0039] ​The third aspect of the present application provides a fluorescence imaging device based on light source intensity adjustment, characterized in that the device comprises a memory, a processor, and a computer program stored in the memory and executable on the processor, and the processor implements the fluorescence imaging method based on light source intensity adjustment as described in any one of the possible implementation manners of the first aspect to the first aspect of the present application.

[0040] The fourth aspect of the present application provides a computer readable storage medium, characterized in that the computer readable storage medium stores a computer program, and the computer program is executed by a processor to implement the fluorescence imaging method based on light source intensity adjustment as described in any one of the possible implementation manners of the first aspect to the first aspect of the present application.

[0041] The present application provides a fluorescence imaging method based on light source intensity adjustment, which is applied to a light intensity adjustment fluorescence imaging device, the light intensity adjustment fluorescence imaging device comprises two modes of a photodynamic diagnosis (PDD) fluorescence mode and a photodynamic white light image mode, and the method comprises the following steps: in the PDD fluorescence mode, when the blue light source is turned on, the green fluorescence band spectrum generated by the excitation of the luciferin is filtered through the trap filter after the blue light is reflected by the observed tissue, so as to reduce the interference of the green fluorescence on the PDD fluorescence contrast; in the photodynamic white light image mode, the red, green and blue light sources are turned on at the same time, wherein the red, green and blue light emitted by the red, green and blue light sources is adjusted in light power according to the pre-set light power through the light compensation module, so that the light reflected by the observed tissue and then passing through the trap filter is white light, the light compensation module controls the light power of the red light and the blue light output to be consistent, and the green light output power is higher than the light power of the red light or the blue light. By combining the trap filter and the light compensation module, the problem that the green fluorescence generated by the excitation of the luciferin affects the contrast of the PDD fluorescence imaging in the PDD fluorescence imaging process is solved, and the color restoration of the traditional white light imaging is also ensured. BRIEF DESCRIPTION OF DRAWINGS

[0042] Figure 1 It is a flowchart of a fluorescence imaging method based on light source intensity adjustment in an embodiment of the present application;

[0043] Figure 2 It is a structural schematic diagram of a fluorescence imaging device based on light source intensity adjustment in an embodiment of the present application;

[0044] Figure 3 It is a structural schematic diagram of a fluorescence imaging device based on light source intensity adjustment in an embodiment of the present application. DETAILED DESCRIPTION

[0045] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present application, but not all the embodiments of the present application. Based on the embodiments of the present application, all the other embodiments obtained by those skilled in the art without creative work fall within the scope of the present application.

[0046] The term "and / or" appearing in the present application can be a description of the association relationship of the associated objects, which means that there can be three relationships, for example, A and / or B can mean that there are three cases of A alone, A and B together, and B alone. In addition, the character " / " in the present application generally represents that the front and rear associated objects are in an "or" relationship.

[0047] The terms "first", "second", and the like in the specification and claims of the present application and the above-described drawings are used to distinguish similar objects, and do not necessarily have to describe a specific order or sequence. It should be understood that the data thus used can be interchanged under appropriate circumstances, so that the embodiments described herein can be implemented in an order other than that illustrated or described herein. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion, for example, a process, method, system, product, or device including a series of steps or modules does not have to be limited to only those steps or modules clearly listed, but can include other steps or modules not clearly listed or inherent to these processes, methods, products, or devices.

[0048] During the process of bladder PDD imaging, the metabolite riboflavin of urine will be excited by excitation light, and green fluorescence will be emitted, thereby interfering with the contrast of PDD fluorescence. The existing method is to change the excitation light band, such as using 500nm as the photosensitizer excitation wavelength, to reduce the excitation efficiency of riboflavin. But changing the excitation wavelength will also cause the fluorescence excitation efficiency of the photosensitizer to decrease, and the fluorescence brightness of PDD imaging to decrease. At the same time, it causes more loss of visible light imaging band, which is not conducive to traditional white light imaging.

[0049] In view of this, referring to Figure 1 , the present application provides a fluorescence imaging method based on light source intensity adjustment, which comprises:

[0050] 101, determining the working mode.

[0051] The fluorescence imaging method based on light source intensity adjustment provided by the present application is applied to a fluorescence imaging device for light intensity adjustment, which is provided with a trap wave filter and a light compensation module.

[0052] Specifically, referring to Figure 2The application also provides a fluorescence imaging device based on light source intensity adjustment, which comprises a blue light source 1, a green light source 2, a red light source 3, a light compensation module 4, an imaging lens group 5, a trap wave filter 6, a photosensitive element 7 and an image processor 8.

[0053] The device has two modes of PDD fluorescence mode and photodynamic white light image mode. The device can also have other modes in the future, which are not limited here.

[0054] 102. In the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observed tissue, and then the green fluorescence spectrum generated by the excitation of the riboflavin is filtered by the trap wave filter to reduce the interference of the green fluorescence on the PDD fluorescence contrast.

[0055] When it is determined that the working mode is the PDD fluorescence mode, the blue light source 1 is turned on, and the green light source 2 and the red light source 3 are turned off. After the blue light emitted by the blue light source 1 irradiates the observed tissue, the imaging lens group 5 is used to collect the PDD fluorescence generated by the excitation in the observed tissue and the green fluorescence spectrum generated by the riboflavin. The trap wave filter 6 is used to filter the riboflavin fluorescence. The PDD fluorescence transmits through the trap wave filter 6 to the photosensitive element 7. The photosensitive element 7 is used to convert the optical signal into an electrical signal and transmit it to the image processor 8 for processing to output a PDD fluorescence image.

[0056] In this way, the green fluorescence spectrum generated by the excitation of the riboflavin is filtered by the trap wave filter 6 to reduce the interference of the green fluorescence on the PDD fluorescence contrast.

[0057] 103. In the photodynamic white light image mode, the red, green and blue light sources are turned on at the same time. The red, green and blue light emitted by the red, green and blue light sources is adjusted by the light compensation module according to the pre-set light power so that the light reflected by the observed tissue and then passing through the trap wave filter is white light. The light compensation module controls the light power of the red light and the blue light to be consistent, and the output power of the green light is higher than that of the red light or the blue light.

[0058] In the photodynamic white light image mode, the blue light source 1, the green light source 2 and the red light source 3 are turned on under the control of the light compensation module 4. The red, green and blue light emitted by the blue light source 1, the green light source 2 and the red light source 3 is adjusted by the light compensation module 4 according to the pre-set light power. The reflected light of the three-color light reflected by the observed tissue is collected by the imaging lens group 5, and then enters the photosensitive element 7 after being filtered by the trap wave filter 6. The photosensitive element 7 is used to convert the optical signal into an electrical signal and transmit it to the image processor 8 for processing to output a white light image.

[0059] The light compensation module 4 controls the light power outputted by the blue light source 1 and the red light source 3 to be consistent, and the green light source 2 outputs power higher than the light power of the blue light source 1 or the red light source 3.

[0060] It should be noted that the light compensation module 4 adjusts the light output ratio of red, green and blue light according to a pre-set light power ratio, wherein the pre-set light power ratio of red, green and blue is related to the transmittance of the notch filter 6.

[0061] Further, if it is determined that the transmittance ratio of red, green and blue light of the notch filter 6 is a:b:a, then the light compensation module 4 controls the output power ratio of red, green and blue light spectrum to be b:a:b, and a and b are positive numbers. For example, if it is determined that the transmittance ratio of red, green and blue light of the notch filter 6 is 1:0.4:1, then it can be determined that the light compensation module 4 controls the output power ratio of red, green and blue light spectrum to be 0.4:1:0.4. This is only an example and is not a limitation of the present application.

[0062] It should be noted that the light power of red, green and blue light emitted by the red, green and blue light sources entering the light sensing element 7 is respectively:

[0063]

[0064] Wherein, P r is the light power of red light entering the light sensing element 7, P g is the light power of green light entering the light sensing element 7, and P b is the light power of blue light entering the light sensing element 7, λ is the wavelength of light, L r (λ) is the output power of red light spectrum, L g (λ) is the output power of green light spectrum, and L b (λ) is the output power of blue light spectrum.

[0065] It can be understood that in the photodynamic white light image mode, the light compensation module 4 controls the output power of red, green and blue light so that the difference percentage of the red, green and blue light power entering the light sensing element 7 and the average power of the three colors is less than or equal to a preset difference percentage threshold. The preset difference percentage threshold can be set according to the actual situation, and for example, it can be set to 10%. This is only an example and is not a limitation of the present application.

[0066] Further, the average power of red, green and blue light is:

[0067]

[0068] ​The difference percentage of the red light power and the average power of the three colors is:

[0069]

[0070] The difference percentage of the green light power and the average power of the three colors is:

[0071]

[0072] The difference percentage of the blue light power and the average power of the three colors is:

[0073]

[0074] wherein, P r is the red light power, P g is the green light power, P b is the blue light power, ΔP r is the difference of the red light power and the average power of the three colors, ΔP g is the difference of the green light power and the average power of the three colors, and ΔP b is the difference of the blue light power and the average power of the three colors, and a is a preset difference percentage threshold, 0

[0075] The application also provides a fluorescence imaging device based on light source intensity adjustment, specifically referring to Figure 2 The device comprises a blue light source 1, a green light source 2, a red light source 3, a light compensation module 4, an imaging lens group 5, a trap wave filter 6, a photosensitive element 7, and an image processor 8.

[0076] In the photodynamic diagnosis (PDD) fluorescence mode, when the blue light source 1 is turned on and the green light source 2 and the red light source 3 are turned off, after the blue light emitted by the blue light source 1 irradiates the observed tissue, the imaging lens group 5 is used to collect the PDD fluorescence generated by the excited observed tissue and the green fluorescence waveband generated by the luciferin, the trap wave filter 6 is used to filter the luciferin fluorescence, the PDD fluorescence transmits through the trap wave filter 6 to the photosensitive element 7, the photosensitive element 7 is used to convert the optical signal into an electrical signal and transmit it to the image processor 8 for processing, so as to output a PDD fluorescence image.

[0077] In the photodynamic white light image mode, the blue light source 1, the green light source 2 and the red light source 3 are all turned on under the control of the light compensation module 4, and the red, green and blue light emitted by the blue light source 1, the green light source 2 and the red light source 3 all pass through the light compensation module 4 for pre-set light power adjustment, the three-color light passes through the observation tissue and is reflected, the reflected light is collected by the imaging lens group 5, and then enters the photosensitive element 7 after being filtered by the wave-trap filter 6. The photosensitive element 7 is used for converting the optical signal into an electrical signal and transmitting to the image processor 8 for processing, and outputting a white light image. The light compensation module 4 controls the light power output by the blue light source 1 and the red light source 3 to be consistent, and the output power of the green light source 2 is higher than that of the blue light source 1 or the red light source 3.

[0078] It should be noted that the light compensation module 4 adjusts the light output of the red, green and blue three-color light according to the pre-set light power ratio, wherein the ratio of the pre-set light power ratio of the red, green and blue three colors is related to the transmittance of the wave-trap filter 6.

[0079] Further, if it is determined that the transmittance ratio of the wave-trap filter 6 for the red, green and blue three-color light is a:b:a, then the output power of the red, green and blue light spectrum is controlled by the light compensation module 4 to be b:a:b, and a and b are both positive numbers.

[0080] The light power of the red, green and blue light emitted by the red, green and blue light source entering the photosensitive element 7 is respectively:

[0081]

[0082] Wherein, P r is the light power of the red light entering the photosensitive element 7, P g is the light power of the green light entering the photosensitive element 7, and P b is the light power of the blue light entering the photosensitive element 7, λ is the wavelength of light, L r (λ) is the output power of the red light spectrum, L g (λ) is the output power of the green light spectrum, and L b (λ) is the output power of the blue light spectrum.

[0083] It should be noted that in the photodynamic white light image mode, the light compensation module 4 controls the output power of the red, green and blue three-color light, so that the difference percentage of the red, green and blue three-color light power entering the photosensitive element 7 and the average power of the three-color light is less than or equal to the pre-set difference percentage threshold.

[0084] The average power of the red, green and blue three-color light is:

[0085]

[0086] ​The difference percentage of the red light power and the average power of the three colors is:

[0087]

[0088] The difference percentage of the green light power and the average power of the three colors is:

[0089]

[0090] The difference percentage of the blue light power and the average power of the three colors is:

[0091]

[0092] wherein, P r is the red light power, P g is the green light power, P b is the blue light power, ΔP r is the difference of the red light power and the average power of the three colors, ΔP g is the difference of the green light power and the average power of the three colors, and ΔP b is the difference of the blue light power and the average power of the three colors, and a is a preset power difference percentage threshold, 0 < a < 1.

[0093] The application provides a fluorescence imaging method and device based on light source intensity adjustment and a storage medium. The method is applied to a light intensity adjusted fluorescence imaging device. The light intensity adjusted fluorescence imaging device includes two modes of a photodynamic diagnosis (PDD) fluorescence mode and a photodynamic white light image mode. The method includes the following steps. In the PDD fluorescence mode, when the blue light source is turned on, the green fluorescence band spectrum generated by the excitation of the luciferin is filtered through a trap filter after the blue light is reflected by the observed tissue, so as to reduce the interference of the green fluorescence on the contrast of the PDD fluorescence. In the photodynamic white light image mode, the red, green and blue light sources are turned on at the same time. The red, green and blue light emitted by the red, green and blue light sources is adjusted in light power according to a preset light power through a light compensation module, so that the light reflected by the observed tissue and then passing through the trap filter is white light. The light compensation module controls the light power of the red light and the blue light to be consistent, and the output power of the green light is higher than the light power of the red light or the blue light. Through the combination of the trap filter and the light compensation module, the problem that the green fluorescence generated by the excitation of the luciferin affects the contrast of the PDD fluorescence imaging in the PDD fluorescence imaging process is solved, and the color restoration of the traditional white light imaging is also ensured.

[0094] Figure 3 A structure schematic diagram of the fluorescence imaging device 20 based on light source intensity adjustment provided by the application is shown in FIG. 1. Figure 3 As shown in FIG. 1, the fluorescence imaging device 20 based on light source intensity adjustment of the embodiment includes at least one processor 201Figure 3 The apparatus 20 for fluorescence imaging based on light source intensity adjustment can comprise, but not limited to, a processor 201, a memory 202. Those skilled in the art can understand that,

[0095] The apparatus 20 for fluorescence imaging based on light source intensity adjustment can comprise, but not limited to, a processor 201, a memory 202. Those skilled in the art can understand that, Figure 3 The apparatus 20 for fluorescence imaging based on light source intensity adjustment is only an example and does not constitute a limitation on the apparatus 20 for fluorescence imaging based on light source intensity adjustment, and can comprise more or less components than those shown, or combine certain components, or different components, for example, it can also comprise input and output devices, network access devices, etc.

[0096] The processor 201 can be a central processing unit (CPU), and can also be other general-purpose processors, a digital signal processor (DSP), an application specific integrated circuit (ASIC), a field-programmable gate array (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor can be a microprocessor or the processor can also be any conventional processor.

[0097] The memory 202 may, in some embodiments, be an internal storage unit of the light source intensity adjustment based fluorescence imaging device 20, such as a hard disk or a memory of the light source intensity adjustment based fluorescence imaging device 20. The light source intensity adjustment based fluorescence imaging device 20 may, in other embodiments, also be an external storage device of the light source intensity adjustment based fluorescence imaging device 20, such as a plug-in hard disk, a smart media card (SMC), a secure digital (SD) card, a flash card, etc. equipped on the light source intensity adjustment based fluorescence imaging device 20. Further, the memory 202 may, in addition, include both the internal storage unit and the external storage device of the light source intensity adjustment based fluorescence imaging device 20. The memory 202 is used to store operation devices, application programs, boot loaders, data, and other programs, such as program codes of the computer programs, etc. The memory 202 may, in addition, be used to temporarily store data that has been output or is to be output.

[0098] Those skilled in the art can clearly understand that, for the convenience and brevity of description, the specific working processes of the above-described system, device and unit can refer to the corresponding processes in the foregoing method embodiments, which will not be described herein.

[0099] The integrated unit, if realized in the form of a software function unit and sold or used as an independent product, can be stored in a computer readable storage medium. Based on such understanding, the technical solutions of the present application essentially or the part that contributes to the prior art or the whole or part of the technical solutions can be embodied in the form of a software product. The computer software product is stored in a storage medium, and includes a plurality of instructions for causing a computer device (which can be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the methods described in the various embodiments of the present application. The foregoing storage medium includes: a U disk, a mobile hard disk, a read-only memory (ROM), a random access memory (RAM), a magnetic disk or an optical disk, and various media that can store program codes.

[0100] In the embodiments provided in the present application, it should be understood that the disclosed methods can be implemented in other ways without exceeding the spirit and scope of the present application. The current embodiments are only exemplary and should not be used as limitations. The specific contents given should not limit the purpose of the present application. For example, some features can be omitted or not executed.

[0101] The technical means disclosed in the application scheme is not limited to the technical means disclosed in the above-mentioned embodiments, and also includes the technical scheme composed of any combination of the above technical features. It should be pointed out that, for ordinary skilled persons in the art, without departing from the principles of the application, some improvements and refinements can be made, which are also considered as the protection scope of the application.

[0102] The above describes in detail the method, device and storage medium for fluorescence imaging based on light source intensity adjustment provided by the embodiments of the application. The principles and implementation manners of the application are described by applying specific examples. The above embodiment description is only used to help understand the method and core idea of the application. Meanwhile, for ordinary skilled persons in the art, according to the idea of the application, the specific implementation manners and application ranges can be changed. In summary, the content of the specification should not be understood as a limitation of the application. Although the application is described in detail with reference to the foregoing embodiments, ordinary skilled persons in the art should understand that they can modify the technical schemes recorded in the foregoing embodiments or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical scheme deviate from the spirit and scope of the technical schemes of the embodiments of the application.

Claims

1. A method for fluorescence imaging based on light source intensity adjustment, characterized in that, The method is applied to a light source intensity adjustment based fluorescence imaging device, the light source intensity adjustment based fluorescence imaging device includes two modes of a photodynamic diagnosis PDD fluorescence mode and a photodynamic white light image mode, and the method includes the following steps: In the PDD fluorescence mode, when the blue light source is turned on, the green fluorescence spectrum generated by the excitation of the riboflavin filtered by the notch filter is filtered after the blue light is reflected by the observed tissue, so as to reduce the interference of the green fluorescence on the PDD fluorescence contrast; In the photodynamic white light image mode, the red, green and blue light sources are turned on at the same time, wherein the red, green and blue light emitted by the red, green and blue light sources is adjusted by the light compensation module according to the pre-set light power, so that the light reflected by the observed tissue and then passing through the notch filter is white light, and the light compensation module controls the light power of the red light and the blue light to be consistent, and the output power of the green light is higher than the light power of the red light or the blue light; The red, green and blue light emitted by the red, green and blue light sources is adjusted by the light compensation module according to the pre-set light power, which specifically includes the following steps: The light compensation module adjusts the light output proportion of the red, green and blue light according to the pre-set light power proportion, wherein the proportion of the red, green and blue light of the pre-set light power proportion is related to the transmittance of the notch filter; The proportion of the red, green and blue light of the pre-set light power proportion is related to the transmittance of the notch filter, which specifically includes the following steps: If it is determined that the transmittance proportion of the red, green and blue light of the notch filter is a:b:a, then the output power ratio of the red light spectrum, the green light spectrum and the blue light spectrum is controlled by the light compensation module to be b:a:b, and a and b are positive numbers.

2. The light source intensity adjustment based fluorescence imaging method according to claim 1, wherein In the photodynamic white light image mode, the light compensation module controls the output power of the red, green and blue light, so that the difference percentage of the red, green and blue light power entering the photosensitive element and the average power of the three colors is less than or equal to the preset difference percentage threshold.

3. The light source intensity adjustment based fluorescence imaging method according to claim 2, wherein Average power of red, green, blue light Is: ； The difference percentage of the red light power and the average power of the three colors is: ； The difference percentage of the green light power and the average power of the three colors is: ； The difference percentage of the blue light power and the average power of the three colors is: ； wherein, is a light power of red light entering the photosensitive element, is a light power of green light entering the photosensitive element, is a light power of blue light entering the photosensitive element, is a difference between the red light power and an average power of the three colors of light, is a difference between the green light power and the average power of the three colors of light, is a difference between the blue light power and the average power of the three colors of light, and m is a preset difference percentage threshold value, .

4. A fluorescence imaging apparatus based on light source intensity adjustment, characterized by, The device includes a blue light source (1), a green light source (2), a red light source (3), a light compensation module (4), an imaging lens group (5), a notch filter (6), a photosensitive element (7), an image processor (8), In the photodynamic diagnosis PDD fluorescence mode, when the blue light source (1) is turned on and the green light source (2) and the red light source (3) are turned off, after the blue light emitted by the blue light source (1) irradiates the observed tissue, the imaging lens group (5) is used to collect the PDD fluorescence generated by the excited observation tissue and the green fluorescence waveband generated by the riboflavin, the notch filter (6) is used to filter the riboflavin fluorescence, the PDD fluorescence transmits through the notch filter (6) to the photosensitive element (7), and the photosensitive element (7) is used to convert the optical signal into an electrical signal and transmit the electrical signal to the image processor (8) for processing to output a PDD fluorescence image; In the photodynamic white light imaging mode, the blue light source (1), the green light source (2), and the red light source (3) are all turned on under the control of the light compensation module (4), and the red, green, and blue light emitted by the blue light source (1), the green light source (2), and the red light source (3) all pass through the light compensation module (4) for pre-set light power adjustment, the reflected light of the three-color light after being reflected by the observed tissue is collected by the imaging lens group (5), and then enters the photosensitive element (7) after being filtered by the notch filter (6), the photosensitive element (7) is used to convert the optical signal into an electrical signal and transmit the electrical signal to the image processor (8) for processing to output a white light image, and the light compensation module (4) controls the light power output by the blue light source (1) and the red light source (3) to be consistent, and the output power of the green light source (2) is higher than that of the blue light source (1) or the red light source (3). The light compensation module (4) adjusts the light output of the red, green, and blue three-color light according to the pre-set light power ratio, wherein the pre-set light power ratio of the red, green, and blue three colors is related to the transmittance of the notch filter (6). If it is determined that the transmittance ratio of the red, green, and blue three-color light of the notch filter (6) is a:b:a, then the output power of the red, green, and blue light spectrum is controlled by the light compensation module (4) to be b:a:b, and a and b are positive numbers.

5. The fluorescence imaging device based on light source intensity adjustment according to claim 4, wherein in the photodynamic white light imaging mode, the light compensation module (4) controls the output power of the red, green, and blue three-color light so that the difference percentage of the red, green, and blue three-color light power entering the photosensitive element (7) and the average power of the three-color light is less than or equal to a preset difference percentage threshold.

6. The fluorescence imaging device based on light source intensity adjustment according to claim 5, wherein the difference percentage of the red light power and the average power of the three-color light is: the difference percentage of the green light power and the average power of the three-color light is: Average power of red, green, blue light Is: ； the difference percentage of the blue light power and the average power of the three-color light is: ； The device comprises a memory, a processor, and a computer program stored in the memory and executable on the processor, and the processor implements the fluorescence imaging method based on light source intensity adjustment according to any one of claims 1 to 3 when executing the computer program. ； ​ ； wherein, is a light power of red light entering the photosensitive element, is a light power of green light entering the photosensitive element, is a light power of blue light entering the photosensitive element, is a difference between the red light power and an average power of the three colors of light, is a difference between the green light power and the average power of the three colors of light, is a difference between the blue light power and the average power of the three colors of light, and m is a preset difference percentage threshold value, .

7. A fluorescence imaging apparatus based on light source intensity adjustment, characterized by, ​ 8. A computer-readable storage medium, characterized in that, The computer readable storage medium stores a computer program, and the computer program is executed by the processor to implement the fluorescence imaging method based on light source intensity adjustment according to any one of claims 1 to 3.

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