Composition for protecting micro-ecological balance of skin and preparation method thereof

Abstract

The invention provides a composition for protecting micro-ecological balance of skin and a preparation method of the composition. Comprising the following components in percentage by weight: 84.12%-92.47% of a solvent, 5%-10% of a bifidus yeast fermentation product lysate, 0.1%-0.5% of tetrahydromethylpyrimidine carboxylic acid, 0.5%-1% of panthenol, 0.5%-1% of lactobacillus / soybean milk fermentation product filtrate, 0.5%-1% of arginine / lysine polypeptide, 0.5%-1% of 1, 2-hexanediol, 0.1%-0.5% of a pH regulator, 0.03%-0.08% of a chelating agent and 0.3%-0.8% of an antioxidant. According to the scheme, probiotic strains which are carefully screened are selected as powerful cell protection factors, an ideal environment for skin flora survival is kept, and the skin health level is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of skin microecological balance, and particularly relates to a composition for protecting skin microecological balance and a preparation method thereof. Background Art

[0002] Skin microecology refers to the ecosystem composed of various microorganisms such as bacteria, fungi, viruses, mites and arthropods, as well as tissues, cells, various secretions, and microenvironments on the surface of the skin. As the largest protective organ of the body, the skin has many parts. The microbiome is an important factor in the skin barrier. The flora can give the skin strong protection, repair and resistance. However, once the flora loses balance, it will cause various skin pathological conditions, such as damaged skin barrier, water-oil imbalance, seborrheic dermatitis, etc.

[0003] With the development of modern society, research and development teams such as L'Oreal and Lancome have begun to explore the connection between skin microecology, skin barrier function and immune response, and the application of probiotics in skin care products has been vigorously promoted. People are constantly affected by endogenous factors (age, diet, work and rest, etc.) and exogenous factors (temperature and humidity, ultraviolet intensity, use of cosmetics, etc.), which leads to the destruction of skin microecology. A healthy microecology can maintain the normal pH level of the skin, regulate the body's immunity, improve cell metabolism, soothe sensitive skin, promote skin repair and inhibit excessive oil secretion. Probiotic technology allows the active substances to be retained to the maximum extent, making it safer and more natural, and therefore favored by consumers. Summary of the invention

[0004] The purpose of the embodiments of this specification is to provide a composition for protecting the microecological balance of the skin and a preparation method thereof.

[0005] To solve the above technical problems, the embodiments of the present application are implemented in the following ways:

[0006] In a first aspect, the present application provides a composition for protecting the microecological balance of the skin, comprising, by weight percentage:

[0007] 84.12%-92.47% solvent, 5-10% bifida yeast fermentation product lysate, 0.1-0.5% ectoine, 0.5-1% panthenol, 0.5-1% lactobacillus / soy milk fermentation product filtrate, 0.5-1% arginine / lysine polypeptide, 0.5-1% 1,2-hexanediol, 0.1-0.5% pH adjuster, 0.03-0.08% chelating agent, 0.3-0.8% antioxidant.

[0008] In one embodiment, the antioxidant is p-hydroxyacetophenone.

[0009] In one embodiment, the chelating agent is disodium EDTA.

[0010] In one embodiment, the pH adjusting agent is arginine.

[0011] In a second aspect, the present application provides a method for preparing a composition for protecting skin microecological balance as in the first aspect, the method comprising:

[0012] Weigh the raw materials according to the weight ratio; the raw materials are divided into phase A raw materials and phase B raw materials, the phase A raw materials are solvents and high temperature resistant raw materials, and the phase B raw materials are active raw materials that are easily volatile or easily inactivated;

[0013] Add the raw materials of phase A into the emulsifying kettle, stir and heat to 70-75°C, and keep stirring at this temperature for 30 minutes until it is completely dissolved;

[0014] When the phase A raw material in the emulsification kettle is stirred and cooled to 40-45°C, the phase B raw material is added to the emulsification kettle and stirred evenly for 10-15 minutes to obtain a composition;

[0015] The composition is filtered to obtain a composition for protecting the microecological balance of the skin.

[0016] In one embodiment, the raw materials of phase A include a solvent, a chelating agent, and an antioxidant.

[0017] In one embodiment, the phase B raw materials include bifida fermentation product lysate, ectoine, panthenol, lactobacillus / soy milk fermentation product filtrate, arginine / lysine polypeptide, 1,2-hexanediol, and a pH adjuster.

[0018] In one embodiment, before adding the phase A raw material into the emulsification kettle, the emulsification kettle is sterilized.

[0019] It can be seen from the technical solutions provided in the embodiments of this specification that the composition for protecting the skin microecological balance provided by the solution is selected from carefully selected probiotic strains, which serve as powerful cell protection factors to maintain an ideal environment for the survival of skin flora and improve the health level of the skin.

[0020] The composition prepared in the present application has good formula stability. Through the synergistic effect between the various components, it is used to prepare skin care products with antioxidant, skin repair, acne improvement and other functions, and has significant development prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of this specification or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this specification. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0022] Figure 1 The present invention provides a schematic flow chart of a method for preparing a composition for protecting the microecological balance of the skin. DETAILED DESCRIPTION

[0023] In order to enable those skilled in the art to better understand the technical solutions in this specification, the technical solutions in the embodiments of this specification will be clearly and completely described below in conjunction with the drawings in the embodiments of this specification. Obviously, the described embodiments are only part of the embodiments of this specification, not all of the embodiments. Based on the embodiments in this specification, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of this specification.

[0024] In the following description, specific details such as specific system structures, technologies, etc. are provided for the purpose of illustration rather than limitation, so as to provide a thorough understanding of the embodiments of the present application. However, it should be clear to those skilled in the art that the present application may also be implemented in other embodiments without these specific details. In other cases, detailed descriptions of well-known systems, devices, circuits, and methods are omitted to prevent unnecessary details from obstructing the description of the present application.

[0025] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present application description without departing from the scope or spirit of the present application. Other embodiments derived from the present application description will be apparent to those skilled in the art. The present application description and examples are exemplary only.

[0026] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0027] The microecology on the surface of the skin is the first skin barrier between the skin and the external environment. The components of the composition for protecting the balance of skin microecology provided in this application are coordinated and matched, and the prebiotic system is used to synergize in multiple dimensions to strengthen the skin barrier, improve the skin's metabolic capacity, and soothe and repair the skin. Studies have shown that microorganisms play a key role in the pathogenesis of inflammatory skin.

[0028] The present invention is further described in detail below with reference to the accompanying drawings and embodiments.

[0029] Example 1

[0030] A method for preparing a composition for protecting the microecological balance of the skin, such as Figure 1 As shown, the following steps are included:

[0031] Step 1. Weigh the raw materials according to weight ratio: 91.65% solvent, 5% bifida yeast fermentation product lysate, 0.1% tetrahydromethylpyrimidine carboxylic acid, 0.5% panthenol, 0.5% lactobacillus / soy milk fermentation product filtrate, 1% arginine / lysine polypeptide, 0.5% 1,2-hexanediol, 0.2% pH adjuster, 0.05% chelating agent, and 0.5% antioxidant.

[0032] The above raw materials can be divided into phase A raw materials and phase B raw materials. Phase A raw materials are solvents and high temperature resistant raw materials, and phase B raw materials are active raw materials that are easily volatile or easily inactivated. Among them, phase A raw materials include solvents, chelating agents, and antioxidants. Phase B raw materials include bifida fermentation product lysate, tetrahydromethylpyrimidine carboxylic acid, panthenol, lactobacillus / soy milk fermentation product filtrate, arginine / lysine polypeptide, 1,2-hexanediol, and pH adjuster.

[0033] Specifically, the solvent may be water.

[0034] The above raw materials, including bifid yeast fermentation product lysate, ectoine, lactobacillus / soy milk fermentation product filtrate, arginine / lysine polypeptide and a composite composition thereof, are used as skin conditioners.

[0035] Among them, the bifid yeast fermentation product lysate is a kind of ingredient commonly used in skin care products, which is composed of bifidobacterium lysate obtained by biotechnology, including metabolites, cytoplasmic components, cell wall components and polysaccharide complexes. It has a variety of skin care effects, such as being able to strengthen the barrier function of the skin, enhance the resistance of the skin, and help resist the adverse effects of the external environment; it has a certain antioxidant effect, can reduce the damage of free radicals to the skin, and delay skin aging; it can also promote DNA repair and improve the self-repair ability of the skin. Because the bifid yeast fermentation product lysate is composed of bifidobacterium lysate obtained by biotechnology, including metabolites, cytoplasmic components, cell wall components and polysaccharide complexes, IL-10 plays a very important role in host defense and immune regulation. Immune homeostasis can prevent ultraviolet rays from causing immunosuppression, so the addition of bifid yeast fermentation product lysate in the composition provided by the present application can reduce the damage to the skin caused by external environmental factors such as ultraviolet rays, drying, pollution, etc., and reduce the stress response of the skin, thereby reducing the imbalance of microbial communities caused by environmental pressure.

[0036] Among them, tetrahydromethylpyrimidine carboxylic acid, selected from the skin microecological protection factors of probiotics specially bred by aerospace, can provide an ideal environment for the benign growth of skin microbial communities, protect cell activity, improve skin defense, and avoid various skin problems caused by imbalance due to external invasion. Because tetrahydromethylpyrimidine carboxylic acid is derived from the fermentation of probiotic strains, enzymes are produced to decompose free fatty acids and maintain skin acidity. It can maintain the types and richness of skin microorganisms, and the distribution of skin microorganisms at various biological classification levels of kingdom, phylum, class, order, family, genus and species can be determined by instrument detection, and the flora structure, diversity and gene function are analyzed. The composition provided by the present application can provide a suitable living environment for skin microorganisms by adding tetrahydromethylpyrimidine carboxylic acid, which helps to maintain the stability of the microbial community. In addition, tetrahydromethylpyrimidine carboxylic acid is derived from the fermentation of probiotic strains, and the culture medium is inoculated for fermentation and culture, and the increase in the number of viable bacteria is detected to promote growth, which can help the colonization and growth of microorganisms on the skin surface, and further promote the balance of skin microecology.

[0037] Among them, the lactobacillus / soy milk fermentation product filtrate is fermented from Japanese black soybean milk through 16 genera and a total of 35 lactobacilli, maximizing the metabolic activity of fermented organisms to obtain derivatives of natural metabolites with the highest content, which has the effect of inhibiting antibody immunoglobulin, can improve skin repair ability, and slow down skin sensitivity. Since the lactobacillus / soy milk fermentation product filtrate is obtained from fermented soy milk, it is beneficial to the growth of lactobacillus and bifidobacterium after sterilization, and lactobacillus has anti-inflammatory activity, which can inhibit the damage to health and skin caused by aflatoxin. Therefore, the composition provided by the present application has certain anti-inflammatory properties by adding lactobacillus / soy milk fermentation product filtrate, which can inhibit the release of inflammatory factors, prevent inflammation from damaging the skin microecology, and maintain the balance of skin microorganisms.

[0038] The above three prebiotic ingredients can be combined to regulate IgE immune protein, reduce skin over-immune response, and reduce skin redness in experimental tests. Therefore, the composition of the present application can also increase the skin barrier function and prevent the invasion of harmful microorganisms by adding three prebiotics.

[0039] Among them, arginine / lysine peptides can quickly activate epidermal cells, replenish nutrients for the skin, stimulate fibroblasts in the dermis, accelerate the synthesis of collagen, resist the invasion of streptococci, repair the barrier, protect the balance of the bacterial flora, and have a variety of skin care and beauty effects.

[0040] Panthenol and 1,2-hexanediol act as humectants.

[0041] Among them, panthenol is a deep penetrating moisturizer that can stimulate the growth of epidermal cells, is easily absorbed and penetrates into the stratum corneum of the skin, and has a powerful effect in repairing the skin barrier. In addition, it is widely used because it also has a certain effect of promoting epithelial absorption, that is, healing wound skin tissue.

[0042] The chelating agent is disodium EDTA, which is an important chelating agent that can chelate metal ions in the solution.

[0043] The antioxidant is p-hydroxyacetophenone, which can be combined with 1,2-hexanediol as a humectant to form a preservative-free system.

[0044] The pH adjuster is arginine.

[0045] Step 2: After sterilizing the emulsifying kettle, add the raw material of phase A into the emulsifying kettle, stir and heat to 70-75°C, and keep stirring at this temperature for 30 minutes until it is completely dissolved.

[0046] Step 3, when the phase A raw material in the emulsification kettle is stirred and cooled to 40-45°C, the phase B raw material is added to the emulsification kettle and stirred evenly for 10-15 minutes to obtain a composition;

[0047] Step 4: Filter the composition to obtain a composition for protecting the microecological balance of the skin.

[0048] Example 2

[0049] A method for preparing a composition for protecting skin microecological balance comprises the following steps:

[0050] Step 1. Weigh the raw materials according to weight proportion: 88.75% solvent, 7.5% bifida fermentation product lysate, 0.25% tetrahydromethylpyrimidine carboxylic acid, 0.5% panthenol, 0.75% lactobacillus / soy milk fermentation product filtrate, 1% arginine / lysine polypeptide, 0.5% 1,2-hexanediol, 0.2% pH adjuster, 0.05% chelating agent, and 0.5% antioxidant.

[0051] Step 2 to step 4 are the same as those in Example 1.

[0052] Example 3

[0053] A method for preparing a composition for protecting skin microecological balance comprises the following steps:

[0054] Step 1. Weigh the raw materials according to weight ratio: 85.75% solvent, 10% bifida yeast fermentation product lysate, 0.5% tetrahydromethylpyrimidine carboxylic acid, 0.5% panthenol, 1% lactobacillus / soy milk fermentation product filtrate, 1% arginine / lysine polypeptide, 0.5% 1,2-hexanediol, 0.2% pH adjuster, 0.05% chelating agent, and 0.5% antioxidant.

[0055] Step 2 to step 4 are the same as those in Example 1.

[0056] Example 4

[0057] A method for preparing a composition for protecting skin microecological balance comprises the following steps:

[0058] Step 1. Weigh the raw materials according to weight proportion: 84.12% solvent, 10% bifida yeast fermentation product lysate, 0.5% tetrahydromethylpyrimidine carboxylic acid, 1% panthenol, 1% lactobacillus / soy milk fermentation product filtrate, 1% arginine / lysine polypeptide, 1% 1,2-hexanediol, 0.5% pH adjuster, 0.08% chelating agent, and 0.8% antioxidant.

[0059] Step 2 to step 4 are the same as those in Example 1.

[0060] Example 5

[0061] A method for preparing a composition for protecting skin microecological balance comprises the following steps:

[0062] Step 1. Weigh the raw materials according to weight proportion: 92.47% solvent, 5% bifida yeast fermentation product lysate, 0.1% tetrahydromethylpyrimidine carboxylic acid, 0.5% panthenol, 0.5% lactobacillus / soy milk fermentation product filtrate, 0.5% arginine / lysine polypeptide, 0.5% 1,2-hexanediol, 0.1% pH adjuster, 0.03% chelating agent, and 0.3% antioxidant.

[0063] Step 2 to step 4 are the same as those in Example 1.

[0064] Example 6

[0065] A method for preparing a composition for protecting skin microecological balance comprises the following steps:

[0066] Step 1. Weigh the raw materials according to weight proportion: 88.75% solvent, 7% bifida yeast fermentation product lysate, 0.25% tetrahydromethylpyrimidine carboxylic acid, 0.75% panthenol, 0.75% lactobacillus / soy milk fermentation product filtrate, 1% arginine / lysine polypeptide, 0.75% 1,2-hexanediol, 0.2% pH adjuster, 0.05% chelating agent, and 0.5% antioxidant.

[0067] Step 2 to step 4 are the same as those in Example 1.

[0068] In order to verify the effect of the present application, the following experiments and tests were conducted to illustrate that the control group in the following experiments and tests was an aqueous product without adding the composition, and the proportions of the remaining components were consistent with those in Example 1.

[0069] 1. Reduce sebum secretion

[0070] Experimental protocol: Sebum measurement is based on the internationally recognized Method (Sebumeter 815, Courage + Khazaka GmbH). According to this method, sebum on the skin is collected in a 64 mm 2 The transparency is measured by inserting the measuring head into the hole of the device. The transmittance indicates the amount of sebum on the surface of the measured area. The microprocessor calculates the result and displays it in μg sebum / cm 2 on your skin.

[0071] Compared with the control group, the combination group had more significant effects, among which Example 3 had the best effect, with the content of skin sebum significantly reduced by 24.6%.

[0072]

[0073] 2. Skin barrier repair

[0074] Test method: 10 subjects aged 30-65 years old, with facial skin meeting TEWL>15g / h / m 2 , the moisture content of the stratum corneum is less than 50 (Corneometer Unit, CU), the facial skin is red, and the facial skin satisfies the lactic acid sting test. The composition provided by the present application is applied to the face for 14 consecutive days, once in the morning and once in the evening. The facial images are taken with VISIA at D0 (before applying the composition) and D14 (14 days after applying the composition), and the area ratio of the red area in the red area is measured.

[0075] By comparing the before and after application of the composition, it was found that the composition provided by the present application had a significant effect in reducing the area ratio of skin redness. Among them, the best effect was Example 3, where the area ratio of skin redness decreased by 4.79%.

[0076]

[0077] 3. Improve skin moisture content

[0078] After the subjects used the composition provided by the present application for 14 days, the moisture content of the subjects' stratum corneum was significantly increased compared with before use. Among them, the moisture content of the stratum corneum of the skin increased the most in Example 3, which increased by 65.27%, which was a very significant difference. It can be shown that the composition provided by the present application can effectively moisturize, maintain and improve skin moisture for a long time.

[0079]

[0080] In summary, the composition for protecting the skin microecological balance provided in the present application plays an active and important role in maintaining the skin microecological balance and keeping the skin healthy through the combined action of multiple mechanisms.

[0081] The present application provides a composition for protecting the balance of skin microecology, which can provide a suitable living environment for skin microorganisms and help maintain the stability of the microbial community; it can reduce the damage to the skin caused by external environmental factors such as ultraviolet rays, dryness, pollution, etc., reduce the stress response of the skin, and thus reduce the imbalance of the microbial community caused by environmental pressure; it also has certain anti-inflammatory properties, which can inhibit the release of inflammatory factors, prevent inflammation from damaging the skin microecology, and maintain the balance of skin microorganisms. In addition, it can enhance the skin barrier function, prevent the invasion of harmful microorganisms, and also help the colonization and growth of beneficial microorganisms on the skin surface, further promoting the balance of skin microecology.

[0082] It should be noted that the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, commodity or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, commodity or device. In the absence of further restrictions, the elements defined by the sentence "comprises a ..." do not exclude the existence of other identical elements in the process, method, commodity or device including the elements.

[0083] Each embodiment in this specification is described in a progressive manner, and the same or similar parts between the embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the system embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiment.

Claims

1. A composition for protecting the microecological balance of the skin, characterized in that: In terms of weight percentage, it includes: 84.12%-92.47% solvent, 5-10% bifida yeast fermentation product lysate, 0.1-0.5% ectoine, 0.5-1% panthenol, 0.5-1% lactobacillus / soy milk fermentation product filtrate, 0.5-1% arginine / lysine polypeptide, 0.5-1% 1,2-hexanediol, 0.1-0.5% pH adjuster, 0.03-0.08% chelating agent, 0.3-0.8% antioxidant.

2. The composition for protecting the skin microecological balance according to claim 1, characterized in that: The antioxidant is p-hydroxyacetophenone.

3. The composition for protecting the skin microecological balance according to claim 1, characterized in that: The chelating agent is disodium EDTA.

4. The composition for protecting the skin microecological balance according to claim 1, characterized in that: The pH regulator is arginine.

5. A method for preparing a composition for protecting skin microecological balance as claimed in any one of claims 1 to 4, characterized in that: The method comprises: Weigh the raw materials according to the weight ratio; the raw materials are divided into phase A raw materials and phase B raw materials, the phase A raw materials are solvents and high temperature resistant raw materials, and the phase B raw materials are active raw materials that are easily volatile or easily inactivated; Add the phase A raw material into the emulsifying kettle, stir and heat to 70-75°C, and keep stirring at this temperature for 30 minutes until it is completely dissolved; When the phase A raw material in the emulsification kettle is stirred and cooled to 40-45° C., the phase B raw material is added to the emulsification kettle and stirred evenly for 10-15 minutes to obtain a composition; The composition is filtered to obtain a composition for protecting the microecological balance of the skin.

6. The preparation method according to claim 5, characterized in that: The phase A raw materials include solvent, chelating agent and antioxidant.

7. The preparation method according to claim 5, characterized in that: The phase B raw materials include bifid yeast fermentation product lysate, ectoine, panthenol, lactobacillus / soy milk fermentation product filtrate, arginine / lysine polypeptide, 1,2-hexanediol, and a pH regulator.

8. The preparation method according to claim 5, characterized in that: Before adding the phase A raw materials into the emulsification kettle, the emulsification kettle is sterilized.

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